A freezing module for pretreatment of paraffin-embedded pathological tissue sections and a manufacturing method thereof

Abstract

The present invention discloses a freezing module for pretreatment of paraffin-embedded pathological tissue sections, comprising an outer square tube, a transverse square tube arranged at both ends of the inner side of the outer square tube, and a plurality of longitudinal square tubes arranged between the two transverse square tubes, wherein a structural plate is arranged on the side of one transverse square tube away from the longitudinal square tube, a decorative plate is arranged on the side of the other transverse square tube away from the longitudinal square tube, a through hole is arranged at the position of the transverse square tube corresponding to the longitudinal square tube, two clearance holes are arranged on the opposite sides of the transverse square tube and the structural plate, and valves are installed in the two clearance holes of the transverse square tube; the transverse square tube and the longitudinal square tube are filled with refrigerant. After the refrigerant of the present invention is frozen, the low temperature is transmitted to the outside through the aluminum alloy square tube, the temperature transmission is uniform, and the freezing effect is good; the surface structure of the freezing module is flat, the contact temperature difference of each part of the pathological tissue is small, and the pathological tissue pretreatment effect is good; through the layer-by-layer surrounding protection of the aluminum alloy square tube, the melting speed of the refrigerant after freezing is slow, and it is more convenient to use.

Description

Technical Field

[0001] The invention relates to the technical field of pathological sections, in particular to a freezing module for pre-processing paraffin-embedded pathological tissue sections and a manufacturing method thereof. Background Art

[0002] In medical examinations, generally speaking, the results obtained through auxiliary examinations such as X-rays, CT, and MRI before surgery are usually unable to determine the nature of the tissue 100%. Only after major resection or pathological examination of local tissue can its nature be determined. Pigmented nevus, lipomas, hemangiomas, malignant tumors and other tissues removed during surgery need further pathological examination to determine whether the preoperative diagnosis is accurate and to guide further treatment measures. During the medical examination, some tissues removed during surgery that need to be determined need to undergo pathological examination. Since the tissue needs to be embedded in paraffin before it can be sliced ​​and tested, the tissue section is also called paraffin section, and the pathology examination report is also called paraffin report. In fact, the full name is paraffin section pathology report, that is, the pathology result.

[0003] In the process of pathological tissue preparation, pathological paraffin tissue sections need to be frozen before slicing to improve the hardness of the pathological tissue and ensure the quality of the slices. In addition, the requirements for the slicing blade are relatively high. After slicing several times, the microstructure of the blade will be damaged, and it will be impossible to cut out smooth pathological tissue sections, thus affecting the detection and use. After the pathological tissue is frozen, the number of times the blade can be used can also be increased.

[0004] In the prior art, pathological tissue sections require the use of cold table products for freezing. However, in reality, the promotion and use of cold tables for pre-processing pathological tissue sections has not been very effective. On the one hand, the cold table uses semiconductor refrigeration, and the product price is relatively high. Many medical institutions are not willing to purchase and use cold tables to freeze pathological tissue sections. On the other hand, the cold table is more troublesome to use. It needs to be pre-set in advance before each use. The pre-set time is between 30 minutes and several hours. The pre-set time is relatively long. If a doctor encounters a situation where pathological sections need to be taken as soon as he or she gets to work, he or she cannot use it in time. In addition, the cold table has high energy consumption and severe frosting, which is inconvenient.

[0005] Therefore, a method that is not very standard but relatively easy to use has been derived, that is, using ice cubes instead of cold plates to freeze pathological tissues, and many doctors prefer this method. Ice cubes are disposable and relatively convenient, but they need to be frozen and made one day in advance. If you forget to make ice cubes, there will be no ice cubes available for pretreatment of pathological tissue sections; the surface of the ice cubes is not flat enough, the contact temperature difference is large, and the freezing effect of pathological tissues is not good; the temperature of the ice cubes is not low enough, and the pretreatment time of pathological tissue freezing is long; ice cubes are easy to melt, and the sustainable use time is short. They are easy to melt and produce water, which has the risk of contaminating tissues, making them inconvenient to use and having shortcomings. Summary of the invention

[0006] In view of the shortcomings of the prior art, the present invention provides a freezing module for pretreatment of paraffin-embedded pathological tissue sections and a manufacturing method thereof, which are used to solve the problem of inconvenience in using a cold stage and ice cubes to pretreatment paraffin-embedded pathological tissues in the prior art.

[0007] In order to solve the above technical problems, the present invention provides the following technical solutions:

[0008] A freezing module for pretreatment of paraffin-embedded pathological tissue sections and a manufacturing method thereof, comprising an outer square tube, transverse square tubes arranged at both ends of the inner side of the outer square tube, and a plurality of longitudinal square tubes arranged between the two transverse square tubes, wherein a structural plate is arranged on the side of one of the transverse square tubes away from the longitudinal square tube, and a decorative plate is arranged on the side of the other transverse square tube away from the longitudinal square tube, a through hole is arranged at a position of the transverse square tube corresponding to the longitudinal square tube, two clearance holes are arranged on the opposite sides of the transverse square tube and the structural plate, and valves are installed in the two clearance holes of the transverse square tube;

[0009] Sealant is applied between the horizontal square tube and the vertical square tube, between the outer square tube and the horizontal square tube and the vertical square tube, and sealant and structural adhesive are also applied between the structural plate and the decorative plate and the horizontal square tube and the outer square tube;

[0010] The transverse square tube and the longitudinal square tube are filled with refrigerant.

[0011] Preferably, the transverse square tube, the longitudinal square tube and the outer casing square tube are all aluminum alloy square tubes, and the overall thickness and wall thickness of the transverse square tube and the longitudinal square tube are equal.

[0012] Preferably, the inner and outer walls of the horizontal square tube, the inner and outer walls of the vertical square tube, and the inner and outer walls of the outer square tube are all provided with an anti-corrosion coating.

[0013] Preferably, a partition plate is provided between the horizontal square tube and the vertical square tube, and through holes are also provided at corresponding positions on the partition plate, and the sealant between the horizontal square tube and the vertical square tube is coated on the surface of the partition plate.

[0014] Preferably, the partition plate and the structural plate are both plastic plates, the decorative plate is a glass plate, and the decorative plate is flush with one end of the outer square tube.

[0015] Preferably, sealing rings are installed between the inner and outer walls of the horizontal square tube and the valve nozzle, and a sealing cover is also installed outside the valve nozzle, and the end surface of the sealing cover does not exceed the end surface of the outer square tube.

[0016] Preferably, the structural adhesive is coated on the outside of the sealant.

[0017] Preferably, the refrigerant is brine.

[0018] A method for manufacturing a freezing module for pre-processing paraffin-embedded pathological tissue sections comprises the following steps:

[0019] S1: Select aluminum alloy square tubes of appropriate size, cut them into fixed lengths according to the lengths of outer square tube 1, horizontal square tube 2 and vertical square tube 3;

[0020] S2: Use ultrasonic testing equipment to ensure that the cut-off spare aluminum alloy square tube has no cracks;

[0021] S3: applying an anti-corrosion coating 10 on the inner and outer walls of the aluminum alloy square tube cut to a fixed length by electrophoresis or brushing, and waiting for solidification;

[0022] S4: drilling holes on the horizontal square tube 2 according to the positions of the clearance hole 6 and the through hole 13;

[0023] S5: Install the valve 7 at the position of the clearance hole 6 on the horizontal square tube 2;

[0024] S6: Apply sealant 8 to the opposite surfaces of the horizontal square tube 2 and the partition plate 11 and the opposite surfaces of the partition plate 11 and the longitudinal square tube 3, adhere and fix the horizontal square tube 2, the longitudinal square tube 3 and the partition plate 11 to each other according to the distribution positions, and wait for solidification;

[0025] S7: Apply sealant 8 to the upper and lower surfaces of the horizontal square tube 2 and the vertical square tube 3 and both ends of the horizontal square tube 2, adhere and fix them in the outer square tube 1, and wait for solidification;

[0026] S8: Apply sealant 8 between the horizontal square tube 2 and the outer square tube 1 and wait for solidification;

[0027] S9: Apply structural adhesive 9 to the outside of the sealant 8 between the horizontal square tube 2 and the outer square tube 1, the inner wall of the outer square tube 1, and the opposite surfaces of the horizontal square tube 2, the structural plate 4 and the decorative plate 5, wait for solidification, and fix by gluing;

[0028] S10: Fill compressed air with a pressure greater than a standard atmospheric pressure through any one of the air valves 7, place the glued and fixed freezing module into clean water, and test the air tightness to ensure that there is no air leakage;

[0029] S11: Release the excess air through any valve 7, and inject a fixed amount of saline refrigerant through a needle-free syringe, and put it into a refrigerator for freezing before use.

[0030] Compared with the prior art, the present invention has the following beneficial effects:

[0031] (1) The present invention fills the refrigerant inside the refrigeration module made of aluminum alloy square tube, which is simple to manufacture and has relatively low manufacturing and use costs;

[0032] (2) After the refrigerant freezes, it transfers low temperature to the outside through the aluminum alloy square tube, the temperature is evenly transferred, and the freezing effect is good;

[0033] (3) The surface structure of the freezing module is flat, the contact temperature difference between different parts of the pathological tissue is small, and the pretreatment effect of the pathological tissue is good;

[0034] (4) The refrigerant is surrounded and protected by layers of aluminum alloy square tubes. The refrigerant melts slowly after freezing, and the refrigeration time can be long.

[0035] (5) The freezing module made of metal aluminum alloy square tube and the saline refrigerant have a cooling effect after freezing. Compared with the direct ice cooling temperature, it is lower and can effectively shorten the pretreatment time of pathological tissue;

[0036] (6) The refrigerant melts inside the freezing module and does not come into contact with the outside. There is no water spill after melting, the thickness of frost is small, and there is no risk of contaminating pathological tissues.

[0037] (7) The freezing module is a rectangular parallelepiped structure with a standard shape. Multiple freezing modules can be modularly spliced ​​and used in combination. Multiple pathological tissues can be pretreated at the same time, which is very practical.

[0038] (8) A valve is provided on the refrigeration module. The refrigerant inside the refrigeration module is not fully filled. The refrigerant can be regularly replenished and exhausted to prevent bulging, and the quality is guaranteed;

[0039] (9) The freezing module can be used for long-term freezing. There is no need to temporarily prepare a freezing module during pathological tissue pretreatment, which is convenient to use. BRIEF DESCRIPTION OF THE DRAWINGS

[0040] Figure 1 It is a front view of the present invention;

[0041] Figure 2 It is a rear view of the present invention;

[0042] Figure 3 A top view of the present invention;

[0043] Figure 4 A top view of the internal structure of the outer sleeve of the present invention;

[0044] Figure 5 It is a top view of a cross-section at a corresponding position of the through hole of the present invention.

[0045] In the figure: 1. outer square tube; 2. horizontal square tube; 3. longitudinal square tube; 4. structural plate; 5. decorative plate; 6. clearance hole; 7. valve; 8. sealant; 9. structural adhesive; 10. anti-corrosion coating; 11. partition plate; 12. sealing cover; 13. through hole. DETAILED DESCRIPTION

[0046] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0047] like Figure 1-5 As shown, the present invention provides a technical solution: a freezing module for pretreatment of paraffin-embedded pathological tissue sections and a manufacturing method, comprising an outer square tube 1, transverse square tubes 2 arranged at both ends of the inner side of the outer square tube 1, and a plurality of longitudinal square tubes 3 arranged between the two transverse square tubes 2, the outer square tube 1 has an outer size of 80*40*18mm, the transverse square tube 2, the longitudinal square tube 3 and the outer square tube 1 are all aluminum alloy square tubes, and the overall thickness and wall thickness of the transverse square tube 2 and the longitudinal square tube 3 are equal, which is conducive to uniformly transmitting low-temperature energy to the outside;

[0048] The horizontal square tube 2 and the vertical square tube 3 are filled with a refrigerant, which is salt water, which is green and environmentally friendly. The salt water refrigerant in the horizontal square tube 2 and the vertical square tube 3 is not fully filled, and 310ml of salt water with a concentration of 5%-6% is filled. The inner and outer walls of the horizontal square tube 2, the inner and outer walls of the vertical square tube 3, and the inner and outer walls of the outer square tube 1 are all provided with an anti-corrosion coating 10 to prevent corrosion caused by internal salt water and external condensed water;

[0049] A partition plate 11 is also provided between the horizontal square tube 2 and the vertical square tube 3. The partition plate 11 can reduce the speed of internal energy transmission to the outside and prolong the freezing time. A through hole 13 is also provided at a corresponding position on the partition plate 11. The sealant 8 between the horizontal square tube 2 and the vertical square tube 3 is coated on the surface of the partition plate 11.

[0050] The partition plate 11 and the structural plate 4 are both plastic plates, and the decorative plate 5 is a colored glass plate, which is beautiful and can be silk-screened with relevant markings and logos, and the decorative plate 5 is flush with one end of the outer square tube 1, and sealing rings are installed between the inner and outer walls of the horizontal square tube 2 and the valve 7, and a sealing cover 12 is also installed outside the valve 7, and the end face of the sealing cover 12 does not exceed the end face of the outer square tube 1;

[0051] A structural plate 4 is provided on one side of the transverse square tube 2 away from the longitudinal square tube 3, and a decorative plate 5 is provided on the other side of the transverse square tube 2 away from the longitudinal square tube 3. A through hole 13 is provided at the position of the transverse square tube 2 corresponding to the longitudinal square tube 3. Two clearance holes 6 are provided on the opposite surfaces of the transverse square tube 2 and the structural plate 4. Valve nozzles 7 are installed in the two clearance holes 6 of the transverse square tube 2.

[0052] Sealant 8 is coated between the transverse square tube 2 and the longitudinal square tube 3, and between the outer square tube 1 and the transverse square tube 2 and the longitudinal square tube 3. Sealant 8 and structural adhesive 9 are also coated between the structural plate 4 and the decorative plate 5 and the transverse square tube 2 and the outer square tube 1. The structural adhesive 9 is coated on the outside of the sealant 8, and the structure has good stability and is sealed layer by layer to prevent refrigerant from overflowing.

[0053] A method for manufacturing a freezing module for pre-processing paraffin-embedded pathological tissue sections comprises the following steps:

[0054] S1: Select aluminum alloy square tubes of appropriate size, cut them into fixed lengths according to the lengths of outer square tube 1, horizontal square tube 2 and vertical square tube 3;

[0055] S2: Use ultrasonic testing equipment to ensure that the cut-off spare aluminum alloy square tube has no cracks;

[0056] S3: applying an anti-corrosion coating 10 on the inner and outer walls of the aluminum alloy square tube cut to a fixed length by electrophoresis or brushing, and waiting for solidification;

[0057] S4: drilling holes on the horizontal square tube 2 according to the positions of the clearance hole 6 and the through hole 13;

[0058] S5: Install the valve 7 at the position of the clearance hole 6 on the horizontal square tube 2;

[0059] S6: Apply sealant 8 to the opposite surfaces of the horizontal square tube 2 and the partition plate 11 and the opposite surfaces of the partition plate 11 and the longitudinal square tube 3, adhere and fix the horizontal square tube 2, the longitudinal square tube 3 and the partition plate 11 to each other according to the distribution positions, and wait for solidification;

[0060] S7: Apply sealant 8 to the upper and lower surfaces of the horizontal square tube 2 and the vertical square tube 3 and both ends of the horizontal square tube 2, adhere and fix them in the outer square tube 1, and wait for solidification;

[0061] S8: Apply sealant 8 between the horizontal square tube 2 and the outer square tube 1 and wait for solidification;

[0062] S9: Apply structural adhesive 9 to the outside of the sealant 8 between the horizontal square tube 2 and the outer square tube 1, the inner wall of the outer square tube 1, and the opposite surfaces of the horizontal square tube 2, the structural plate 4 and the decorative plate 5, wait for solidification, and fix by gluing;

[0063] S10: Fill compressed air with a pressure greater than a standard atmospheric pressure through any one of the air valves 7, place the glued and fixed freezing module into clean water, and test the air tightness to ensure that there is no air leakage;

[0064] S11: Release the excess air through any valve 7, and inject a fixed amount of saline refrigerant through a needle-free syringe, and put it into a refrigerator for freezing before use.

[0065] The following is the test data. The E-type freezing module is a freezing module without a partition plate 11, and the F-type freezing module is a freezing module with a partition plate 11. The test is stopped when the surface temperature of the freezing module rises to 0°C.

[0066] Table 1 Surface temperature of the freezing module when the freezing time is 13 hours and the detection environment is 22.3℃

[0067]

[0068]

[0069]

[0070] When the freezing module is just taken out of the refrigerator, the cold energy inside the freezing module has not been completely transferred to the surface, and the surface temperature has not yet reached the lowest temperature; during the detection process, the cold energy inside the freezing module is gradually transferred outward, and the external temperature gradually increases due to the evaporation of surface condensed water and heat exchange with the ambient air; it is worth noting that there are nonlinear changes in the evaporation rate of condensed water on the surface of the freezing module and the heat exchange rate of the ambient air, which causes the detected temperature to fluctuate.

[0071] The lowest temperature detected by the E-type freezing module reached -13.1℃ at 7:19, and the surface temperature remained below 0℃ for 5 hours and 18 minutes; the lowest temperature detected by the F-type freezing module reached -12.6℃ at 7:29, and the surface temperature remained below 0℃ for 6 hours and 30 minutes. In summary, both models can maintain a temperature below 0℃ for at least half a day, which is enough for medical personnel to perform pathological tissue freezing pretreatment.

[0072] Working principle:

[0073] The inner and outer walls of the transverse square tube 2, the longitudinal square tube 3 and the outer square tube 1 are all provided with an anti-corrosion coating 10, which effectively prevents corrosion caused by the later brine refrigerant and external condensed water and protects the freezing module; the transverse square tube 2 and the longitudinal square tube 3 are fixed inside the outer square tube 1 by means of sealant 8, and a partition plate 11 is installed between the transverse square tube 2 and the longitudinal square tube 3 to delay the cold energy of the internal freezing refrigerant from being transferred outward and prolong the service life; on the basis of the sealant 8, the structural strength is increased by means of structural adhesive 9 to fix the structural plate 4 and the decorative plate 5; the brine refrigerant is injected into the transverse square tube 2 and the longitudinal square tube 3 through the valve 7 by means of a syringe, and the refrigerant is not fully filled to prevent the freezing module from being deformed due to the increase in the freezing volume of the refrigerant; the refrigerant is regularly replenished and the excess air is regularly discharged through the valve 7, which can effectively protect the freezing module from deformation and ensure normal freezing use; after freezing, the surface temperature of the freezing module can be kept below 0°C for up to half a day at room temperature, which is sufficient for the pretreatment of pathological tissue sections, and the structure is simple and easy to use.

[0074] It should be noted that, in this article, terms such as "comprises", "includes" or any other variations thereof are intended to cover non-exclusive inclusion, so that a process, method, article or apparatus that includes a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or apparatus.

[0075] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A freezing module for pretreatment of paraffin-embedded pathological tissue sections, characterized in that: The invention comprises an outer square tube (1), transverse square tubes (2) arranged at both ends of the inner side of the outer square tube (1), and a plurality of longitudinal square tubes (3) arranged between the two transverse square tubes (2), wherein a structural plate (4) is arranged on the side of one of the transverse square tubes (2) away from the longitudinal square tube (3), and a decorative plate (5) is arranged on the side of the other transverse square tube (2) away from the longitudinal square tube (3), a through hole (13) is arranged at a position of the transverse square tube (2) corresponding to the longitudinal square tube (3), two clearance holes (6) are arranged on the opposite surfaces of the transverse square tube (2) and the structural plate (4), and valves (7) are installed in the two clearance holes (6) of the transverse square tube (2); Sealant (8) is applied between the transverse square tube (2) and the longitudinal square tube (3), and between the outer square tube (1) and the transverse square tube (2) and the longitudinal square tube (3); sealant (8) and structural adhesive (9) are also applied between the structural plate (4) and the decorative plate (5) and the transverse square tube (2) and the outer square tube (1); The transverse square tube (2) and the longitudinal square tube (3) are filled with refrigerant.

2. A freezing module for pretreatment of paraffin-embedded pathological tissue sections according to claim 1, characterized in that: The transverse square tube (2), the longitudinal square tube (3) and the outer casing square tube (1) are all aluminum alloy square tubes, and the overall thickness and wall thickness of the transverse square tube (2) and the longitudinal square tube (3) are equal.

3. A freezing module for pretreatment of paraffin-embedded pathological tissue sections according to claim 2, characterized in that: The inner and outer walls of the transverse square tube (2), the inner and outer walls of the longitudinal square tube (3), and the inner and outer walls of the outer square tube (1) are all provided with an anti-corrosion coating (10).

4. A freezing module for pretreatment of paraffin-embedded pathological tissue sections according to claim 1, characterized in that: A partition plate (11) is also provided between the transverse square tube (2) and the longitudinal square tube (3), and a through hole (13) is also provided at a corresponding position on the partition plate (11), and a sealant (8) between the transverse square tube (2) and the longitudinal square tube (3) is coated on the surface of the partition plate (11).

5. A freezing module for pretreatment of paraffin-embedded pathological tissue sections according to claim 4, characterized in that: The partition plate (11) and the structural plate (4) are both plastic plates, the decorative plate (5) is a glass plate, and the decorative plate (5) is flush with one end of the outer square tube (1).

6. A freezing module for pretreatment of paraffin-embedded pathological tissue sections according to claim 1, characterized in that: Sealing rings are installed between the inner and outer walls of the transverse square tube (2) and the valve nozzle (7), and a sealing cover (12) is also installed outside the valve nozzle (7), and the end surface of the sealing cover (12) does not exceed the end surface of the outer square tube (1).

7. A freezing module for pretreatment of paraffin-embedded pathological tissue sections according to claim 1, characterized in that: The structural adhesive (9) is coated on the outside of the sealant (8).

8. A freezing module for pre-processing paraffin-embedded pathological tissue sections according to claim 1, characterized in that: The refrigerant is brine.

9. A method for manufacturing a freezing module for pretreatment of paraffin-embedded pathological tissue sections, characterized in that: The following steps are involved: S1: Select aluminum alloy square tubes of appropriate size, cut them into fixed lengths according to the lengths of outer square tube 1, horizontal square tube 2 and vertical square tube 3; S2: Use ultrasonic testing equipment to ensure that the cut-off spare aluminum alloy square tube has no cracks; S3: applying an anti-corrosion coating 10 on the inner and outer walls of the aluminum alloy square tube cut to a fixed length by electrophoresis or brushing, and waiting for solidification; S4: drilling holes on the horizontal square tube 2 according to the positions of the clearance hole 6 and the through hole 13; S5: Install the valve 7 at the position of the clearance hole 6 on the horizontal square tube 2; S6: Apply sealant 8 to the opposite surfaces of the horizontal square tube 2 and the partition plate 11 and the opposite surfaces of the partition plate 11 and the longitudinal square tube 3, adhere and fix the horizontal square tube 2, the longitudinal square tube 3 and the partition plate 11 to each other according to the distribution positions, and wait for solidification; S7: Apply sealant 8 to the upper and lower surfaces of the horizontal square tube 2 and the vertical square tube 3 and both ends of the horizontal square tube 2, adhere and fix them in the outer square tube 1, and wait for solidification; S8: Apply sealant 8 between the horizontal square tube 2 and the outer square tube 1 and wait for solidification; S9: Apply structural adhesive 9 to the outside of the sealant 8 between the horizontal square tube 2 and the outer square tube 1, the inner wall of the outer square tube 1, and the opposite surfaces of the horizontal square tube 2, the structural plate 4 and the decorative plate 5, wait for solidification, and fix by gluing; S10: Fill compressed air with a pressure greater than a standard atmospheric pressure through any one of the air valves 7, place the glued and fixed freezing module into clean water, and test the air tightness to ensure that there is no air leakage; S11: Release the excess air through any valve 7, and inject a fixed amount of saline refrigerant through a needle-free syringe, and put it into a refrigerator for freezing before use.

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