Method for promoting rapid germination of nuts by enriching functional active components

Abstract

The invention discloses a method for promoting rapid germination of nuts by enriching functional active components, and belongs to the technical field of functional component enrichment. The method comprises the following steps: soaking nuts in a mixed solution of methyl jasmonate and salicylic acid; performing ultrasonic treatment on the soaked nuts; sprouting the nuts subjected to ultrasonic treatment, and radiating the sprouted nuts by using a UV-B lamp; spraying the nuts with a spraying solution during germination; therefore, rapid germination of nuts and enrichment of functional active components are realized. The method is easy to operate and free of environmental pollution, functional active ingredients such as gamma-aminobutyric acid, folic acid, melatonin and gamma-tocopherol can be enriched in the nut buds, a new enrichment method is developed for enrichment of the functional active ingredients, and the method has important application value and prospects in the fields of nut germination and functional active ingredient enrichment.

Description

Technical Field

[0001] The invention belongs to the technical field of functional component enrichment, and particularly relates to a method for enriching functional active components to promote rapid germination of nuts. Background Art

[0002] Nuts are rich in nutrients, such as hazelnuts, walnuts, almonds, macadamia nuts, pecans, etc., containing unsaturated fatty acids, fiber, vitamins, folic acid, tocopherol, thiamine, minerals such as magnesium, copper, potassium and selenium, carotenoids, antioxidants and phytosterol compounds and other beneficial ingredients. Intake of nuts is beneficial to health and can prevent and treat some risk factors associated with chronic diseases, such as changes in blood sugar and lipid metabolism, oxidative stress and inflammation.

[0003] γ-aminobutyric acid (GABA), also known as 4-aminobutyric acid, is widely present in organisms, including plants, animals and microorganisms. GABA in animals is mainly present in nerve tissues and is the most important inhibitory neurotransmitter in the central nervous system of mammals. It mainly exists in brain tissues in the form of homocarnosine. 30% of the neurons in the human brain contain GABA, which affects almost all neural activities of the body.

[0004] Folic acid, also known as vitamin B9, is a water-soluble vitamin. Chemically, it is composed of pteridine, para-aminobenzoic acid, and glutamic acid. Folic acid has extremely important functions in the human body. It is mainly involved in the synthesis of DNA and RNA, and plays a key role in cell division and growth. This makes it particularly important for pregnant women because it can prevent fetal neural tube defects such as spina bifida and congenital diseases such as anencephaly. In addition, folic acid acts as a coenzyme in the metabolism of homocysteine ​​to methionine, which helps to lower homocysteine ​​levels in the blood and reduce the risk of cardiovascular disease. The human body cannot synthesize folic acid on its own and must obtain it from food or supplements.

[0005] Melatonin is an amine hormone secreted by the pineal gland in the human brain, and some are also produced from the retina, gastrointestinal tract and other parts. Melatonin can improve sleep, shorten the time of waking up before bedtime and the time of falling asleep, and improve sleep quality. Melatonin also has antioxidant properties. It can remove free radicals in the body, such as hydroxyl free radicals and hydrogen peroxide, which helps protect cells from oxidative damage and plays a protective role on various cells such as nerve cells. In addition, melatonin may also have a certain effect in regulating immune function and cardiovascular function. The biological functions of melatonin, especially its health function as a dietary supplement, have been widely studied at home and abroad, showing that it has many physiological functions such as promoting sleep, adjusting jet lag, anti-aging, regulating immunity, and anti-tumor.

[0006] γ-Tocopherol is a form of vitamin E, and its main function is antioxidant. It can remove free radicals in the body, such as peroxyl radicals, hydroxyl radicals, etc., thereby protecting the lipid membranes, DNA and proteins of cells from oxidative damage caused by free radicals. Especially in the cell membrane, γ-tocopherol can terminate the free radical chain reaction by providing hydrogen atoms. In addition, γ-tocopherol may also have positive significance in preventing cardiovascular diseases. It can reduce the oxidation of low-density lipoprotein cholesterol, and oxidized low-density lipoprotein cholesterol is a risk factor for atherosclerosis. At the same time, some studies have also found that it may play a role in anti-inflammatory and anti-cancer aspects.

[0007] The sprouting of nuts can promote positive changes in the nutrients inside the nuts, thereby increasing the content of some ingredients. For example, the content of ingredients such as vitamin C is relatively scarce in unsprouted nuts, but it will increase significantly after sprouting. At the same time, the bioavailability of minerals is also improved. Sprouting can also enhance the activity of enzymes in nuts. These enzymes play a key role in the human digestion process. They help break down the components in nuts that are originally difficult to digest by the human body, thereby reducing the digestive burden of the gastrointestinal tract to a certain extent. Some nuts may generate new antioxidants after sprouting. These newly produced antioxidants can participate in the free radical scavenging reaction in the body, help to improve the body's antioxidant capacity, and thus play a potential positive role in maintaining the normal physiological function of cells and preventing chronic diseases.

[0008] Since the seed coat of nuts is thick, it acts as a physical barrier to prevent water and oxygen from being absorbed into the seeds, both of which are necessary for germination. Therefore, the germination time of nuts is usually longer, which makes the nuts prone to rot and the loss of beneficial ingredients. Moreover, in the current nut category, there is also a lack of a method to increase the content of functional active ingredients in nuts (such as gamma-aminobutyric acid, folic acid, melatonin, gamma-tocopherol, etc.). Summary of the invention

[0009] The invention soaks nuts in a mixed solution of methyl jasmonate and salicylic acid, and after soaking, ultrasonic treatment is performed on the nuts to change the dense structure of the nut surface, activate the activity of the nuts, and promote the early germination of the nuts; activates enzymes related to the synthesis of functional components, and simultaneously uses a mixed solution of methyl jasmonate, salicylic acid and NaCl to spray the nuts for germination, and during the germination process, UV-B technology is used to treat the nuts, so as to significantly promote the enrichment of functional active components in nut buds.

[0010] The technical solution of the present invention is as follows:

[0011] A method for enriching functional active ingredients and promoting rapid germination of nuts comprises the following steps:

[0012] The nuts are soaked in a mixed solution of methyl jasmonate and salicylic acid; the soaked nuts are subjected to ultrasonic treatment; the nuts after ultrasonic treatment are germinated and irradiated with UV-B lamps; and the nuts are sprayed with a spray solution during the germination period, so as to achieve rapid germination of the nuts and enrichment of functional active ingredients.

[0013] In the above method, in the mixed solution of methyl jasmonate and salicylic acid, the concentration of methyl jasmonate is selected from 10 to 15 μM, preferably 10 μM; the concentration of salicylic acid is selected from 30 to 50 μM, preferably 50 μM.

[0014] In the above method, the soaking conditions are as follows: soaking temperature is 25 to 30° C., and soaking time is 12 to 24 hours.

[0015] In the above method, the ultrasonic treatment conditions are selected from: ultrasonic power of 200-250W, ultrasonic time of 10-50min; preferably: ultrasonic power of 240W, ultrasonic time of 30min.

[0016] In the above method, the germination temperature is selected from 25 to 30° C.; and the germination time is selected from 8 to 12 days.

[0017] In the above method, the radiation conditions of the UV-B lamp are selected from: wavelength of 313nm, radiation distance of 50cm, radiation intensity of 8μW·cm -2 The radiation time is 1 to 2 hours per day.

[0018] In the above method, the spray solution is a mixed solution of methyl jasmonate, salicylic acid and NaCl; the concentration of methyl jasmonate is selected from 10 to 15 μM, preferably 10 μM; the concentration of salicylic acid is selected from 30 to 50 μM, preferably 50 μM; the concentration of NaCl is selected from 20 to 50 mM, preferably 40 mM.

[0019] In the above method, the spraying frequency is selected from: spraying once every 3 to 5 hours, and the spraying solution is replaced every 24 hours.

[0020] In the present invention, the functional active ingredients are selected from one or more of γ-aminobutyric acid, folic acid, melatonin and γ-tocopherol.

[0021] The invention provides nut sprouts rich in functional active ingredients obtained by the method.

[0022] The beneficial effects of the present invention are:

[0023] During the germination process of nuts, the combination of UV-B irradiation and NaCl solution treatment can effectively alleviate the corruption of nuts during the germination process. At the same time, its stress effect on the germinated nuts can promote the enrichment of functional active ingredients in nuts.

[0024] Soaking nuts in methyl jasmonate solution or salicylic acid solution before they germinate can promote early germination of the nuts, while reducing the stress effects of UV-B and NaCl stress on the germinating nuts during the germination process.

[0025] Ultrasonic treatment of nut seeds changes the dense structure of the nut surface, activates the activity of the nuts, prompts them to germinate earlier, increases the germination speed, and also increases the germination rate of nuts; activating seeds before stress makes them more resistant to external stress during germination and maintain normal growth state, while activating enzymes related to the synthesis of functional ingredients, further increasing the content of functional active ingredients in nuts.

[0026] Treating nuts with methyl jasmonate or salicylic acid solution during germination significantly increased the biomass of the nuts and further increased the content of functional active ingredients in the nuts.

[0027] In summary, the method of the present invention is simple to operate, has no environmental pollution, and can enrich functional active ingredients such as γ-aminobutyric acid, folic acid, melatonin, and γ-tocopherol in nut buds, thereby opening up a new enrichment method for the enrichment of functional active ingredients. It has important application value and prospects in the field of nut germination and enrichment of functional active ingredients. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 The effects of different treatment conditions on the germination time of nuts; from left to right are Examples 1 to 9;

[0029] Figure 2 The effects of different treatment conditions on the bud length of each nut are shown; from left to right are Examples 1 to 9;

[0030] Figure 3 The effects of different treatment conditions on the GABA content in the nut sprouts; from left to right are the unsprouted nut group, Examples 1 to 9;

[0031] Figure 4 The effect of different treatment conditions on the content of functional active ingredients in each nut sprout; from left to right are the unsprouted nut group, Example 1 to Example 9. DETAILED DESCRIPTION

[0032] The nuts used in the following embodiments of the present invention include hazelnuts, walnuts, pecans, macadamia nuts, Australian almonds 25 / 27 varieties, and Australian almonds 27 / 30 varieties.

[0033] Other materials used in the present invention, if not otherwise stated, can be obtained through commercial channels. Other terms used in the present invention, unless otherwise specified, generally have the meanings commonly understood by those of ordinary skill in the art. The present invention is further described in detail below in conjunction with specific examples and with reference to data. The following examples are only for illustrating the present invention, and are not intended to limit the scope of the present invention in any way.

[0034] Example 1

[0035] The steps for germination of nuts and enrichment of functional active ingredients are as follows:

[0036] Select 20 different varieties of nuts with full grains, high germination rate and no damage. Soak the selected nuts in distilled water at 30°C for 24 hours. Place the soaked nuts in a tray for germination and irradiate with UV-B lamp (UV-B radiation conditions are as follows: wavelength 313nm, radiation distance 50cm, radiation intensity 8μW·cm -2 ), irradiated for 1 hour every day; during the germination period, the nut kernels were sprayed with distilled water every 3 hours, and the spray solution was changed every 24 hours. The germination period lasted for 10 days. The complete and non-rotten nut buds were selected and quickly frozen at -80°C for 5 hours, and then placed in a freeze dryer with a temperature set at -50°C and freeze-dried for 3 days; then ground with a freeze grinder to obtain nut powder. The nut powder was placed in a desiccator and stored for later use.

[0037] Example 2

[0038] The steps for germination of nuts and enrichment of functional active ingredients are as follows:

[0039] Select 20 different varieties of nuts with full grains, high germination rate and no damage. Soak the selected nuts in distilled water at 30°C for 24 hours. Ultrasonicate the soaked nuts (ultrasonic power of 240W, ultrasonic time of 30min), place the ultrasonic nuts in a tray for germination, and irradiate with UV-B lamp (UV-B radiation conditions are as follows: wavelength of 313nm, radiation distance of 50cm, radiation intensity of 8μW·cm -2 ), irradiated for 1 hour every day; during the germination period, the nut kernels were sprayed with distilled water every 3 hours, and the spray solution was changed every 24 hours. The germination period lasted for 10 days. The complete and non-rotten nut buds were selected and quickly frozen at -80°C for 5 hours, and then placed in a freeze dryer with a temperature set at -50°C and freeze-dried for 3 days; then ground with a freeze grinder to obtain nut powder. The nut powder was placed in a desiccator and stored for later use.

[0040] Example 3

[0041] The steps for germination of nuts and enrichment of functional active ingredients are as follows:

[0042] Select 20 different varieties of nuts with full grains, high germination rate and no damage. Soak the selected nuts in a 10μM methyl jasmonate solution at 30°C for 24 hours. Place the soaked nuts in a tray for germination and irradiate with UV-B lamp (UV-B radiation conditions are as follows: wavelength 313nm, radiation distance 50cm, radiation intensity 8μW·cm -2 ), irradiated for 1 hour every day; during the germination period, the nut kernels were sprayed with 10 μM methyl jasmonate solution, sprayed every 3 hours, and the spray solution was changed every 24 hours, and the germination period lasted for 10 days. Select the complete and non-rotten nut buds and quickly freeze them at -80°C for 5 hours, then place them in a freeze dryer, set the temperature to -50°C, and freeze-dry for 3 days; then grind them with a freeze grinder to obtain nut powder. Place the nut powder in a desiccator and store it for later use.

[0043] Example 4

[0044] The steps for germination of nuts and enrichment of functional active ingredients are as follows:

[0045] Select 20 different varieties of nuts with full grains, high germination rate and no damage. Soak the selected nuts in a 50μM salicylic acid solution at 30°C for 24 hours. Place the soaked nuts in a tray for germination and irradiate with UV-B lamp (UV-B radiation conditions are as follows: wavelength 313nm, radiation distance 50cm, radiation intensity 8μW·cm -2 ), irradiated for 1 hour every day; during the germination period, the nut kernels were sprayed with 50μM salicylic acid solution, sprayed every 3 hours, and the spray solution was changed every 24 hours, and the germination period lasted for 10 days. The complete and non-rotten nut buds were selected and quickly frozen at -80℃ for 5 hours, then placed in a freeze dryer, set at -50℃, and freeze-dried for 3 days; then ground with a freeze grinder to obtain nut powder. The nut powder was placed in a desiccator and stored for later use.

[0046] Example 5

[0047] The steps for germination of nuts and enrichment of functional active ingredients are as follows:

[0048] Select 20 different varieties of nuts with full grains, high germination rate and no damage. Soak the selected nuts in distilled water at 30°C for 24 hours. Place the soaked nuts in a tray for germination and irradiate with UV-B lamp (UV-B radiation conditions are as follows: wavelength 313nm, radiation distance 50cm, radiation intensity 8μW·cm -2), irradiated for 1 hour every day; during the germination period, the nut kernels were sprayed with 40mM NaCl solution, sprayed every 3 hours, and the spray solution was changed every 24 hours, and the germination period lasted for 10 days. The complete and non-rotten nut buds were selected and quickly frozen at -80℃ for 5 hours, and then placed in a freeze dryer, set at -50℃, and freeze-dried for 3 days; then ground with a freeze grinder to obtain nut powder. The nut powder was placed in a desiccator and stored for later use.

[0049] Example 6

[0050] The steps for germination of nuts and enrichment of functional active ingredients are as follows:

[0051] Select 20 different varieties of nuts with full grains, high germination rate and no damage. Soak the selected nuts in a 10μM methyl jasmonate solution at 30°C for 24 hours. Place the soaked nuts in a tray for germination and irradiate with UV-B lamp (UV-B radiation conditions are as follows: wavelength 313nm, radiation distance 50cm, radiation intensity 8μW·cm -2 ), irradiated for 1 hour every day; during the germination period, the nut kernels were sprayed with a mixed solution of 10 μM methyl jasmonate + 40 mM NaCl, sprayed every 3 hours, and the spray solution was changed every 24 hours, and the germination lasted for 10 days. Select the complete and non-rotten nut buds and quickly freeze them at -80°C for 5 hours, then place them in a freeze dryer, set the temperature to -50°C, and freeze-dry for 3 days; then grind them with a freeze grinder to obtain nut powder. The nut powder was placed in a desiccator and stored for later use.

[0052] Example 7

[0053] The steps for germination of nuts and enrichment of functional active ingredients are as follows:

[0054] Select 20 different varieties of nuts with full grains, high germination rate and no damage. Soak the selected nuts in a 50μM salicylic acid solution at 30°C for 24 hours. Place the soaked nuts in a tray for germination and irradiate with UV-B lamp (UV-B radiation conditions are as follows: wavelength 313nm, radiation distance 50cm, radiation intensity 8μW·cm -2 ), irradiated for 1 hour every day; during the germination period, the nut kernels were sprayed with a mixed solution of 50μM salicylic acid + 40mM NaCl, sprayed every 3 hours, and the spray solution was changed every 24 hours, and the germination lasted for 10 days. The complete and non-rotten nut buds were selected and quickly frozen at -80℃ for 5 hours, then placed in a freeze dryer, set at -50℃, and freeze-dried for 3 days; then ground with a freeze grinder to obtain nut powder. The nut powder was placed in a desiccator and stored for later use.

[0055] Example 8

[0056] The steps for germination of nuts and enrichment of functional active ingredients are as follows:

[0057] Select 20 different varieties of nuts with full grains, high germination rate and no damage. Soak the selected nuts in distilled water at 30°C for 24 hours. Ultrasonicate the soaked nuts (ultrasonic power of 240W, ultrasonic time of 30min), place the ultrasonicated nuts in a tray for germination, and irradiate with UV-B lamp (UV-B radiation conditions are as follows: wavelength of 313nm, radiation distance of 50cm, radiation intensity of 8μW·cm -2 ), irradiated for 1 hour every day; during the germination period, the nut kernels were sprayed with 40mM NaCl solution, sprayed every 3 hours, and the spray solution was changed every 24 hours, and the germination period lasted for 10 days. The complete and non-rotten nut buds were selected and quickly frozen at -80℃ for 5 hours, and then placed in a freeze dryer, set at -50℃, and freeze-dried for 3 days; then ground with a freeze grinder to obtain nut powder. The nut powder was placed in a desiccator and stored for later use.

[0058] Example 9

[0059] The steps for germination of nuts and enrichment of functional active ingredients are as follows:

[0060] Select 20 different varieties of nuts with full grains, high germination rate and no damage. Soak the selected nuts in a mixed solution of 10μM methyl jasmonate + 50μM salicylic acid at 30°C for 24 hours. Ultrasonicate the soaked nuts (ultrasonic power of 240W, ultrasonic time of 30min), place the ultrasonicated nuts in a tray for germination, and irradiate with UV-B lamp (UV-B radiation conditions are as follows: wavelength of 313nm, radiation distance of 50cm, radiation intensity of 8μW·cm -2 ), irradiated for 1 hour every day; during the germination period, the nut kernels were sprayed with a mixed solution of 10μM methyl jasmonate + 50μM salicylic acid + 40mM NaCl, sprayed every 3 hours, and the spray solution was changed every 24 hours, and the germination lasted for 10 days. Select the complete and non-rotten nut buds and quickly freeze them at -80℃ for 5 hours, then place them in a freeze dryer, set the temperature to -50℃, and freeze-dry for 3 days; then grind them with a freeze grinder to obtain nut powder. Place the nut powder in a desiccator and store it for later use.

[0061] 1. Effects of different treatment conditions on the germination time of nuts

[0062] The results are as follows Figure 1 shown.

[0063] from Figure 1It can be seen that compared with nuts germinated with distilled water alone, the germination time of nuts treated with ultrasound or salicylic acid was significantly shortened, the germination time of nuts treated with NaCl-UV-B combined stress was significantly increased, and the germination time of nuts treated with methyl jasmonate did not change significantly. Compared with NaCl-UV-B combined stress alone, methyl jasmonate treatment on this basis had no significant effect on the germination time, while ultrasound or salicylic acid treatment on this basis could significantly shorten the germination time of nuts. Compared with ultrasound or salicylic acid combined with NaCl-UV-B stress, the germination time of nuts treated with salicylic acid or ultrasound and methyl jasmonate on this basis was further shortened.

[0064] This shows that soaking nuts in methyl jasmonate or salicylic acid solution and then subjecting them to ultrasonic treatment can change the dense structure of the nut surface, activate the activity of the nuts, and promote early germination of the nuts; activate the enzymes related to the synthesis of functional ingredients, and at the same time combine methyl jasmonate mixed with NaCl solution or salicylic acid mixed with NaCl solution to germinate the nuts, and treat the nuts with UV-B technology during the germination process, which can significantly promote the early germination of the nuts.

[0065] 2. Effects of different treatment conditions on the bud length of each nut

[0066] The results are as follows Figure 2 shown.

[0067] from Figure 2It can be seen that compared with nuts germinated with distilled water alone, the sprout length of nuts treated with ultrasound or salicylic acid increased significantly (hazelnut 38.68% and 43.37%, walnut 22.51% and 24.72%, pecan 38.17% and 44.34%, macadamia 45.27% and 52.7%, Australian 25 / 27 variety almond 29.45% and 32.77%, Australian 27 / 30 variety almond 27.16% and 33.06%), and the sprout length of nuts treated with NaCl-UV-B combined stress decreased significantly (hazelnut 24.81%, walnut 23.43%, pecan 29.15%, macadamia 41.52%, Australian 25 / 27 variety almond 23.04%, Australian 27 / 30 variety almond 21.47%), while there was no significant change in the sprout length of nuts treated with methyl jasmonate. Compared with the combined stress of NaCl-UV-B alone, the use of methyl jasmonate on this basis had no significant effect on the sprout length, while the use of ultrasound or salicylic acid on this basis significantly increased the sprout length of nuts (hazelnut 61.69% and 52.44%, walnut 43.53% and 38.36%, pecan 58.2% and 55.76%, macadamia 129.78% and 110.65%, Australian 25 / 27 variety almond 34.57% and 38.88, Australian 27 / 30 variety almond 32.09% and 37.87%). Compared with ultrasound or salicylic acid combined with NaCl-UV-B stress, the bud length of nuts treated with salicylic acid or ultrasound and methyl jasmonate was significantly increased (hazelnut 45.24% and 36.93%, walnut 32.92% and 37.89%, pecan 56.59% and 59.04%, macadamia 92.64% and 109.08%, Australian 25 / 27 variety almond 58.65% and 53.73%, Australian 27 / 30 variety almond 55.28% and 48.76%).

[0068] This shows that soaking nuts in methyl jasmonate or salicylic acid solution and then subjecting the nuts to ultrasonic treatment can change the dense structure of the nut surface, activate the activity of the nuts, and promote early germination of the nuts; activate the enzymes related to the synthesis of functional ingredients, and at the same time combine methyl jasmonate mixed with NaCl solution or salicylic acid mixed with NaCl solution to germinate the nuts, and treat the nuts with UV-B technology during the germination process, which can significantly increase the sprout length of the germinated nuts.

[0069] 3. Effects of different treatment conditions on GABA content in nut sprouts

[0070] The results are as follows Figure 3 shown.

[0071] Depend on Figure 3It can be seen that compared with unsprouted nuts, the GABA content of sprouted nuts is significantly increased (hazelnuts 108.66%, walnuts 226.8%, pecans 77.22%, macadamia nuts 68.64%, Australian 25 / 27 variety almonds 373.67%, Australian 27 / 30 variety almonds 104.53%). Compared with nuts germinated with distilled water alone, the GABA content of nuts treated with ultrasound increased significantly (hazelnuts 12.1%, walnuts 48.03%, pecans 75.24%, macadamia nuts 24.74%, Australian 25 / 27 almonds 76.11%, Australian 27 / 30 almonds 100.31%). The GABA content of nuts treated with salicylic acid or methyl jasmonate also increased significantly (hazelnuts 22.56% and 20.19%, walnuts 64.44% and 55.43%, pecans 98.56% and 79 .24%, macadamia 39% and 37.74%, Australian 25 / 27 almonds 74.88% and 92.15%, Australian 27 / 30 almonds 123.34% and 130.41%), the GABA content of nuts treated with NaCl-UV-B combined was also significantly increased (hazelnuts 15.54%, walnuts 56.22%, pecans 85.81%, macadamia 30.9%, Australian 25 / 27 almonds 83.15%, Australian 27 / 30 almonds 157.36%). Compared with the germinated nuts under NaCl-UV-B combined stress, the GABA content in germinated hazelnuts treated with ultrasound or salicylic acid or methyl jasmonate was significantly increased (hazelnut 29.79%, 39.17% and 33.24%, walnut 26.99%, 20.87% and 25.12%, pecan 0.91%, 53.49% and 64.2%, macadamia 28.41%, 30.66% and 35.37%, Australian 25 / 27 variety almond 11.22%, 22.2% and 15.82%, Australian 27 / 30 variety almond 6.34%, 13.11% and 9.63%). Compared with ultrasound or salicylic acid or methyl jasmonate combined with NaCl-UV-B stress treatment, the GABA content of nuts in the ultrasound + salicylic acid + methyl jasmonate + NaCl-UV-B combined treatment group was further significantly increased.

[0072] This shows that soaking nuts in methyl jasmonate or salicylic acid solution and then subjecting the nuts to ultrasonic treatment to change the dense structure of the nut surface, activate the activity of the nuts, and promote early germination of the nuts; activating enzymes related to the synthesis of functional ingredients, and at the same time combining methyl jasmonate mixed with NaCl solution or salicylic acid mixed with NaCl solution to germinate the nuts, and treating the nuts with UV-B technology during the germination process, can significantly increase the GABA content in the germinated nuts.

[0073] 4. Effects of different treatment conditions on the content of functional active ingredients in nut sprouts

[0074] The results are as follows Figure 4 shown.

[0075] Depend on Figure 4 It can be seen that compared with ungerminated nuts, germination treatment can increase the content of functional active ingredients in nuts, and the content of functional active ingredients in germinated nuts after treatment is further significantly increased, but the content of functional active ingredients in nuts treated with NaCl-UV-B combined stress is significantly reduced, and on this basis, the content of functional active ingredients in nuts treated with combined stress will be further increased.

[0076] from Figure 4 A can be seen that compared with ungerminated hazelnuts, the folic acid content of germinated hazelnuts increased by 80.91%, and the folic acid content of germinated hazelnuts treated with ultrasound or salicylic acid or methyl jasmonate or NaCl-UV-B combined stress increased by 47.95%, 55.61%, 61.72% and 67.66% compared with the hazelnuts germinated with distilled water alone. Compared with the germinated hazelnuts under NaCl-UV-B combined stress, the folic acid content of germinated hazelnuts treated with ultrasound or salicylic acid or methyl jasmonate on this basis increased by 14.98%, 29.34% and 21.08%. Compared with ultrasound or salicylic acid or methyl jasmonate combined with NaCl-UV-B stress treatment, the folic acid content of the ultrasound + salicylic acid + methyl jasmonate + NaCl-UV-B combined treatment group was further significantly increased.

[0077] from Figure 4 B shows that compared with ungerminated walnuts, the melatonin content of germinated walnuts increased by 44.64%, while the melatonin content of germinated walnuts treated with ultrasound or salicylic acid or methyl jasmonate or NaCl-UV-B combined stress increased by 23.63%, 33.17%, 29.54% and 35.61% compared with walnuts germinated with distilled water alone. Compared with germinated walnuts treated with NaCl-UV-B combined stress, the melatonin content of germinated walnuts treated with ultrasound or salicylic acid or methyl jasmonate increased by 12.38%, 18.9% and 17.08% on this basis. Compared with ultrasound or salicylic acid or methyl jasmonate combined with NaCl-UV-B stress treatment, the melatonin content of the ultrasound + salicylic acid + methyl jasmonate + NaCl-UV-B combined treatment group was further significantly increased.

[0078] from Figure 4C It can be seen that compared with the ungerminated pecans, the γ-tocopherol content of the germinated pecans increased by 42.68%, and the γ-tocopherol content of the germinated pecans treated with ultrasound or salicylic acid or methyl jasmonate or NaCl-UV-B combined stress increased by 23.59%, 25.11%, 27.42% and 27.56% compared with the pecans germinated with distilled water alone. Compared with the germinated pecans under NaCl-UV-B combined stress, the γ-tocopherol content of the germinated pecans treated with ultrasound or salicylic acid or methyl jasmonate on this basis increased by 17.14%, 11.09% and 14.99%. Compared with the ultrasound or salicylic acid or methyl jasmonate combined with NaCl-UV-B stress treatment, the γ-tocopherol content of the ultrasound + salicylic acid + methyl jasmonate + NaCl-UV-B combined treatment group was further significantly increased.

[0079] This shows that soaking nuts in methyl jasmonate or salicylic acid solution and then subjecting them to ultrasonic treatment can change the dense structure of the nut surface, activate the activity of the nuts, and promote early germination of the nuts; activate the enzymes related to the synthesis of functional ingredients, and at the same time combine methyl jasmonate mixed with NaCl solution or salicylic acid mixed with NaCl solution to germinate the nuts, and use UV-B technology to treat the nuts during the germination process, which can significantly increase the content of functional active ingredients in the germinated nuts.

[0080] Based on the above implementation content, the present invention provides a method for enriching functional active ingredients to promote rapid germination of nuts, comprising the following steps:

[0081] (1) Material selection

[0082] Choose nuts with full grains, high germination rate and no damage as raw materials;

[0083] (2) Soaking treatment

[0084] Soak the selected nuts in a mixed solution of 10-15 μM methyl jasmonate + 30-50 μM salicylic acid at a temperature of 25-30°C for 12-24 hours;

[0085] (3) Ultrasonic treatment

[0086] The soaked nuts are subjected to ultrasonic treatment, with an ultrasonic power of 200 to 250 W and an ultrasonic time of 10 to 50 minutes;

[0087] (4) Germination

[0088] The ultrasonic nut kernels were placed in a tray for germination at a germination temperature of 25-30°C and irradiated with UV-B lamp (UV-B irradiation conditions were as follows: wavelength 313 nm, irradiation distance 50 cm, irradiation intensity 8 μW·cm -2), irradiate for 1 to 2 hours every day, and during the irradiation period, spray the nut seeds with a mixed solution of 10 to 15 μM methyl jasmonate + 30 to 50 μM salicylic acid + 20 to 50 mM NaCl, spray once every 3 to 5 hours, replace the spray solution every 24 hours, and germinate for 8 to 12 days; thereby achieving the enrichment of functional active ingredients in the nut buds;

[0089] (5) Preparation of nut powder

[0090] Select complete and non-rotten nut buds and quickly freeze them at -70 to -80°C for 5 to 10 hours, then place them in a freeze dryer, set the temperature at -50 to -90°C, and freeze dry them for 2 to 3 days; then grind them with a grinder to obtain nut powder;

[0091] (6) Place the nut powder in a dryer for subsequent experimental use.

[0092] The above is only a preferred embodiment of the present invention, and does not limit the present invention in other forms. Any technician familiar with the profession may use the above disclosed technical content to change or modify it into an equivalent embodiment with equivalent changes. However, any simple modification, equivalent change and modification made to the above embodiment according to the technical essence of the present invention without departing from the technical solution of the present invention still belongs to the protection scope of the technical solution of the present invention.

Claims

1. A method for enriching functional active ingredients to promote rapid germination of nuts, characterized in that: The steps include: The nuts are soaked in a mixed solution of methyl jasmonate and salicylic acid; the soaked nuts are subjected to ultrasonic treatment; the nuts after ultrasonic treatment are germinated and irradiated with UV-B lamps; and the nuts are sprayed with a spray solution during the germination period, so as to achieve rapid germination of the nuts and enrichment of functional active ingredients.

2. The method according to claim 1, characterized in that In the mixed solution of methyl jasmonate and salicylic acid, the concentration of methyl jasmonate is selected from 10 to 15 μM, and the concentration of salicylic acid is selected from 30 to 50 μM.

3. The method according to claim 1, characterized in that The soaking conditions are as follows: soaking temperature 25-30° C., soaking time 12-24 h.

4. The method according to claim 1, characterized in that: The ultrasonic treatment conditions are selected from: ultrasonic power of 200 to 250 W, and ultrasonic time of 10 to 50 min.

5. The method according to claim 1, characterized in that The germination temperature of the germination is selected from 25 to 30° C.; and the germination time is selected from 8 to 12 days.

6. The method according to claim 1, characterized in that The radiation conditions of the UV-B lamp are selected from: wavelength of 313nm, radiation distance of 50cm, radiation intensity of 8μW·cm -2 The radiation time is 1 to 2 hours per day.

7. The method according to claim 1, characterized in that The spray solution is a mixed solution of methyl jasmonate, salicylic acid and NaCl; the concentration of the methyl jasmonate is selected from 10 to 15 μM, the concentration of the salicylic acid is selected from 30 to 50 μM, and the concentration of the NaCl is selected from 20 to 50 mM.

8. The method according to claim 1, characterized in that The spraying frequency of the spraying is selected from: spraying once every 3 to 5 hours, and the spraying solution is replaced every 24 hours.

9. The method according to claim 1, characterized in that: The functional active ingredients are selected from one or more of γ-aminobutyric acid, folic acid, melatonin and γ-tocopherol.

10. Nut sprouts rich in functional active ingredients obtained by the method according to any one of claims 1 to 9.

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