Method for promoting spawning of queen Italian bees by feeding juvenile hormone to queen Italian bees

Abstract

According to the method for promoting spawning by feeding the Italian bee queen with juvenile hormone, the Italian bee queen is quantitatively fed with a JHIII sugar aqueous solution with the concentration of 500 micrograms / milliliter, each queen bee is fed with 20 microliters of JHIII sugar aqueous solution, that is, the effective mass of JHIII fed to each queen bee is 10 micrograms, and the result shows that after the method is adopted, the content of the JHIII sugar aqueous solution in the Italian bee queen is increased, and the content of the JHIII sugar aqueous solution in the Italian bee queen is increased. The egg laying quantity and quality of the Italian bee queen bee are remarkably improved, and meanwhile the Italian bee queen bee can still keep a good physiological state.

Description

Technical Field

[0001] The invention relates to the technical field of bee breeding, in particular to a method for promoting egg laying by feeding Italian honey bee queen bee with juvenile hormone. Background Art

[0002] In traditional beekeeping, the queen bee's egg-laying ability directly affects the reproductive efficiency of the bee colony and the economic benefits of beekeeping. Due to factors such as environmental changes, improper feeding and management, and queen bee health, the queen bee's egg-laying capacity often fluctuates. In order to improve the number and quality of queen bee eggs, researchers have tried a variety of methods, including optimizing feed formulas and adjusting environmental conditions. However, these methods have limited effects and often rely on long-term management of the environment and feed. Therefore, how to improve egg-laying efficiency by regulating the queen bee's physiological state has become an urgent problem to be solved in the field of beekeeping.

[0003] Juvenile hormone, as a hormone that promotes insect growth and development, has been used in breeding research of various insects. In bee breeding, appropriate juvenile hormone can promote the development of queen bee ovaries and improve its egg-laying ability. The prior art has not been able to fully utilize juvenile hormone to achieve efficient egg-laying of honey bee queens. Therefore, the present invention provides a new technology for promoting egg-laying by feeding Italian honey bee queens with juvenile hormone. Summary of the invention

[0004] The purpose of the present invention is to provide a method for promoting egg laying of an Italian honey bee queen by feeding it with juvenile hormone. The method can significantly improve the egg laying efficiency of the queen bee, increase the reproductive capacity of the bee colony, and thus improve the production efficiency of bee breeding.

[0005] To achieve the above object, the present invention adopts the following technical solutions.

[0006] A method for promoting egg laying by feeding Italian honey bee queen bee with juvenile hormone, wherein the juvenile hormone is added to queen bee feed to prepare a feed solution and then the solution is fed to the queen bee.

[0007] The juvenile hormone is R-JHⅢ and S-JHⅢ.

[0008] The juvenile hormone is R-JHⅢ.

[0009] The juvenile hormone concentration of the feed solution is 500 μg / ml.

[0010] The feeding amount in step (2) is 20 μL.

[0011] The specific operation of the feeding is as follows: the queen bee is taken out from the beehive and is attached to a clean culture dish with double-sided tape, the prepared feed solution containing juvenile hormone is fed to the queen bee with a pipette, and the queen bee is returned to the original beehive after eating.

[0012] The feeding is performed by starving the queen bee before feeding.

[0013] The method provided by the present invention for promoting egg laying by feeding juvenile hormone to the Italian honey bee queen has the following advantages:

[0014] 1. Significantly improve egg-laying efficiency: By regulating the hormone level in the queen bee's body, the queen bee's egg-laying ability can be significantly improved, thereby promoting the rapid reproduction of the bee colony.

[0015] 2. Improve production efficiency: enhance the reproductive capacity of bee colonies and improve the honey production and reproduction efficiency of bee breeding.

[0016] 3. Reduce environmental dependence: Compared with traditional environmental regulation methods, the method of the present invention relies more on the regulation of endogenous hormones, reducing the impact of the external environment.

[0017] 4. Strong adaptability: This method is suitable for Italian bee breeding under different environmental conditions and has strong universality. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 This is a diagram of the spawning area marking method described in the present invention.

[0019] Figure 2 This is a feeding schematic diagram of the present invention.

[0020] Figure 3 To compare the egg production of queen bees fed different feeds. DETAILED DESCRIPTION

[0021] The present invention is further described in detail below with reference to the embodiments and drawings.

[0022] Example

[0023] In an experimental bee colony, Italian honey bee queens were selected and the following steps were followed: Juvenile hormone solution was prepared: Juvenile hormones R-JHⅢ and S-JHⅢ were selected and dissolved in a 50% sugar solution at a concentration of 500μg / ml. The bee colony was divided into 4 groups for feeding, among which the CK group was fed with a 50% sugar solution; the SCK group was fed with a 50% sugar solution containing 2% acetonitrile solution; the R-JHⅢ group was fed with a 50% sugar solution containing 500μg / ml R-JHⅢ; and the S-JHⅢ group was fed with a 50% sugar solution containing 500μg / ml S-JHⅢ.

[0024] Mark the beehive: Prepare 12 beehives and mark them, No. 1, No. 2, ... No. 12, place 4 honeycombs in each beehive, mark each honeycomb, for example: No. 1 honeycomb: 1-1, 1-2, 1-3... Finally, add up the number of eggs laid in each honeycomb as the total number of eggs laid in the beehive. The control group CK is No. 1-3, the solvent control group SCK is No. 4-6, R-JHⅢ is No. 7-9, and S-JHⅢ is No. 10-12.

[0025] Feeding the queen bee: take out the queen bee, record which box the queen bee comes from, put it back to the original hive after feeding it medicine, starve the queen bee after taking it out, then stick the queen bee on a clean culture dish with double-sided tape to fix it, then slowly press the pipette to feed 20μl of feed solution to the queen bee, wait for the queen bee to finish eating the feed solution and then put it back to the original hive.

[0026] Feeding frequency: In this experiment, the queen bee was fed with juvenile hormone sugar water solution only once.

[0027] Regularly record the number of eggs: The egg period of Italian honey bees is 3 days, and they will develop into larvae on the 4th day. After feeding the medicine on the same day, draw the area where the queen bee lays eggs (without counting the number of eggs). On the 4th day after the medicine, draw the area where the queen bee newly lays eggs and compare it with the last area (at this time, the eggs circled in the last area have reached the larval stage and developed into larvae, so the number of eggs counted on the 4th day are the newly laid eggs after the medicine is used, which is the first official count). Then the number of eggs laid by the queen bee in 3 days can be counted, and the number of eggs can be recorded regularly, with a frequency of counting every 3 days.

[0028] The experiment was divided into 4 times, and each beehive was in the same position each time, so that each beehive was tested in the same environment. After the first test was completed, the second test was carried out, and so on. Each test was separated by 3 days.

[0029] Health monitoring: Check the queen bee's health every three days to ensure that she has no health problems caused by excessive hormones; check the quality of the eggs every three days to see if the newly laid eggs can develop into larvae normally.

[0030] Statistics of egg laying of each group of Italian honey bee queens after feeding

[0031] Unit: Pellet

[0032]

[0033] The experimental results showed that there was no significant difference in the egg-laying amount between the solvent control group SCK and the control group CK, and the solvent had no effect on the queen bee's egg-laying. By feeding Italian honey bee queens with juvenile hormone, the number of eggs laid by the queen bee was significantly increased compared with the control group, and the queen bee was in good health, with no obvious side effects. The quality of each new egg was good, plump and shiny, and the new eggs could develop normally into healthy larvae. The results of the four experiments showed that the total egg-laying amount of the R-JHⅢ was increased by 1.4, 1.2, 0.7 and 1.0 times compared with the CK control group; the total egg-laying amount of the S-JHⅢ was increased by 1.4, 0.9, 0.1 and 0.2 times compared with the CK control group. The total egg-laying results of the four surveys showed that the total egg-laying amount of CK was 7301 eggs, the total egg-laying amount of SCK was 7848 eggs, the total egg-laying amount of R-JHⅢ was as high as 14822 eggs, and the total egg-laying amount of S-JHⅢ was 11340 eggs. The total egg-laying amount of R-JHⅢ was twice that of CK, and the total egg-laying amount of S-JHⅢ was 1.6 times that of CK. It can be seen that JHⅢ can significantly promote the queen bee's egg-laying, and R's performance is more efficient.

[0034] The results of the third survey found that the egg-laying amount of R-JHⅢ and S-JHⅢ increased less than that of the other three surveys. The possible reason for this is that we only put 4 honeycombs in each experimental beehive for them to lay eggs. Since the queen bee laid a huge number of eggs in the first and second surveys after the JH treatment, there were fewer holes available for the queen bee to lay eggs in the third survey, resulting in a lack of obvious increase in the queen bee's egg-laying amount. However, R-JHⅢ increased dramatically in the fourth survey, which also shows that its effect in promoting egg-laying is long-lasting.

[0035] The invention provides a method for feeding Italian honey bee queen bees with juvenile hormone to promote the laying of eggs, wherein the Italian honey bee queen bees are specifically fed: the queen bees are taken out and starved and fixed with double-sided adhesive, and accurately fed with a pipette; a specific juvenile hormone: JHⅢ; a specific juvenile hormone mass: 500 μg / ml JHⅢ sugar water solution is fed, each queen bee is fed 20 μl, that is, each queen bee is fed with a total effective mass of 10 μg of JHⅢ. The queen bees are fed once every 3 days.

[0036] The method can significantly increase the number and quality of eggs laid by queen bees, thereby promoting the reproduction of bee colonies and improving the production efficiency of bee breeding. Through the present invention, the problem of insufficient egg laying by queen bees in traditional bee breeding can be effectively solved, providing a new technical means for the bee breeding industry.

Claims

1. A method for promoting egg laying by feeding juvenile hormone to an Italian honey bee queen, characterized in that: The juvenile hormone is added to the queen bee feed to prepare a feed solution and then fed to the queen bee.

2. The method for promoting egg laying by feeding juvenile hormone to an Italian honey bee queen according to claim 1, characterized in that: The juvenile hormone is JHⅢ.

3. The method for promoting egg laying by feeding juvenile hormone to an Italian honey bee queen according to claim 1 or 2, characterized in that: The juvenile hormone is R-JHⅢ.

4. The method for promoting egg laying by feeding juvenile hormone to an Italian honey bee queen according to claim 3, characterized in that: The juvenile hormone concentration of the feed solution is 500 μg / ml.

5. The method for promoting egg laying by feeding juvenile hormone to an Italian honey bee queen according to claim 4, characterized in that: The feeding amount in step (2) is 20 μL.

6. The method for promoting egg laying by feeding juvenile hormone to an Italian honey bee queen according to claim 4, characterized in that: The specific operation of the feeding is as follows: the queen bee is taken out from the beehive and is attached to a clean culture dish with double-sided tape, the prepared feed solution containing juvenile hormone is fed to the queen bee with a pipette, and the queen bee is returned to the original beehive after eating.

7. The method for promoting egg laying by feeding juvenile hormone to an Italian honey bee queen according to claim 4, characterized in that: The feeding comprises starvation treatment of the queen bee before feeding.

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