Application of D-ribose in improving salt stress resistance of plants

Abstract

The invention belongs to the technical field of biological breeding, and particularly relates to application of D-ribose to improvement of salt stress resistance of plants. The invention provides application of D-ribose in improving salt stress resistance of plants. As an important plant metabolite, D-ribose plays a key role in the growth and development stage of plants. Under the salt stress condition, by applying the D-ribose, growth of main roots, plant heights and leaves of plants can be promoted, the inhibition effect of salt stress on crop growth is relieved, and the D-ribose has a protection effect on later flowering of the plants and generation of seeds. The application method is simple, and the use effect is remarkable.

Description

Technical Field

[0001] The invention belongs to the technical field of biological breeding, and particularly relates to the application of D-ribose in improving plant resistance to salt stress. Background Art

[0002] Excessive soil salinity poses a serious threat to plant growth and food production, and has a far-reaching impact on my country's agricultural production and sustainable agricultural development. First, a high-salt environment disrupts the normal metabolism of plants and reduces their ability to absorb water, which in turn causes leaf stomata to close and bud growth to be blocked. In addition, excessive salt will inhibit the synthesis of chlorophyll and the activity of enzymes in the photosynthetic apparatus, especially the formation of chlorophyll, causing plant leaves to appear pale and yellow, seriously affecting the synthesis of carbohydrates in photosynthesis, and thus hindering the healthy growth and development of plants. At the same time, high salt also weakens the respiration of plants, increases respiratory consumption in the body, and reduces the net photosynthetic rate, which is further detrimental to plant growth.

[0003] Secondly, excessive salt can also cause ion toxicity, among which K + The intracellular accumulation of Zn2+, Mn2+ and Mo2+ ions will damage the cell structure, including damage to the membrane structure and organelles, affect the absorption of beneficial nutrients, change the photosynthesis process, accelerate leaf senescence, and inhibit enzyme activity. With the increasing severity of soil salinity problems, the damage it has caused to the social economy and agricultural ecosystems has continued to deepen, and has become a major obstacle to the development of my country's agriculture. Therefore, how to effectively alleviate the salt stress problem faced by plants has become an important issue that needs to be solved urgently.

[0004] At all stages of growth and development, plants continuously interact with the environment, and during this process, many primary and secondary metabolites are produced in plants. These metabolites show specific or non-specific expression patterns in different developmental periods and tissue parts of plants, and serve as key factors in responding to various biotic and abiotic stresses. Salt stress, as a significant abiotic stress, has a negative effect on plant growth and can trigger dynamic adjustments in metabolite levels in plants. These adjustments involve a variety of metabolites, such as basic metabolites such as amino acids and carbohydrates, as well as secondary metabolites such as phenolic acids, flavonoids, terpenes, steroids and alkaloids. Studies have shown that these metabolites play a central role in the mechanism of plant adaptation to biotic and abiotic stresses. In particular, sucrose, as a key metabolite, plays an important role in the process of plant adaptation to salt stress. For example, under salt stress, the sucrose synthesis activity and sucrose content of sweet sorghum increased significantly, which may mean that sucrose is involved in the plant's response mechanism to salt stress. This phenomenon reveals that changes in metabolite synthesis and content in plants are adaptive responses of plants to adverse growth conditions. Therefore, exploring and utilizing these metabolites that change significantly under salt stress provides new perspectives and approaches for developing solutions for plant salt tolerance.

[0005] When soil salt accumulates significantly, there will be excessive accumulation of reactive oxygen species in plants, triggering oxidative stress reactions. To resist the damage of excessive reactive oxygen species, plants need to activate a series of antioxidant defense mechanisms to maintain redox balance in cells. When exploring how to increase plant yield and improve their growth conditions under salt stress, it is crucial to explore which metabolites can protect plants from salt stress. The metabolites that are specifically expressed by plants under salt stress may be the factors that play a key role in adversity. Therefore, under salt stress conditions, the exogenous application of these specific metabolites provides a new strategy for plants to cope with salt stress. The rational use of these functional metabolites is expected to open up new ways to improve plant growth and development under salt stress conditions. However, the current research on metabolites in plant salt stress response is still insufficient, and the functions of many potential metabolites in alleviating plant stress growth still need to be further explored. Summary of the invention

[0006] The purpose of the present invention is to provide the application of D-ribose in improving the resistance of plants to salt stress, and the application prospect is broad.

[0007] In order to achieve the above object, the technical solution adopted by the present invention is:

[0008] Application of D-ribose in improving plant resistance to salt stress.

[0009] Furthermore, the concentration of the salt stress is 50-200 mmol / L.

[0010] Furthermore, the specific method of the application is:

[0011] D-ribose is applied to the seeds or seedlings of the plant.

[0012] Furthermore, the administration concentration of the D-ribose is 0.5-1.5 mmol / L.

[0013] Furthermore, the administration concentration of the D-ribose is 1 mmol / L.

[0014] Furthermore, the specific method of applying D-ribose to plant seeds is: sterilizing the plant seeds and then sowing them in a culture medium containing D-ribose for culturing.

[0015] Furthermore, the specific method of applying D-ribose to plant seedlings is: adding D-ribose to water or culture medium, and then spraying the leaves of the plant seedlings.

[0016] Furthermore, the plant is Arabidopsis, cotton, corn, wheat, or soybean.

[0017] Furthermore, the frequency of spraying is once every 5 days, and the amount of spraying is based on completely wetting all leaves.

[0018] Furthermore, the D-ribose can promote the growth of the main root, plant height and leaves of the plant, and effectively alleviate the inhibitory effect of salt stress on plant growth and development.

[0019] The beneficial technical effects of the present invention are:

[0020] The present invention provides an application of D-ribose in improving plant resistance to salt stress. As a plant metabolite, D-ribose can play an important role in the growth and development of plants. Under salt stress conditions, the application of D-ribose can greatly promote the growth of crop taproots, plant height and leaves, effectively reduce the negative impact of salt stress, and ensure the smooth progress of later flowering and seed production. The application method is simple and the use effect is significant. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 This is the phenotype of Arabidopsis plants after culturing for 7 days using the three culture media in Example 1;

[0022] Figure 2 is the phenotype diagram of each group of Arabidopsis plants after being cultured for different time periods in Example 2; wherein Figure 2 A is the phenotype diagram of each group of Arabidopsis plants after 10 days of cultivation in Example 2, Figure 2 B is the phenotype of each group of Arabidopsis plants after 60 days of culture in Example 2;

[0023] Figure 3is the phenotype diagram of each group of cotton plants after 14 days of cultivation in Example 3;

[0024] Figure 4 This is the phenotype diagram of each group of corn plants after 14 days of cultivation in Example 4. DETAILED DESCRIPTION

[0025] The following is a further detailed description of the present invention in conjunction with specific preferred embodiments, and it cannot be determined that the specific implementation of the present invention is limited to these descriptions. For those of ordinary skill in the technical field to which the present invention belongs, without departing from the concept of the present invention, several simple deductions or replacements can also be made, all of which should be deemed to belong to the protection scope of the present invention. The specific conditions not indicated in the embodiments are carried out according to conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used, unless otherwise specified, are conventional products obtained through commercial channels.

[0026] Example 1

[0027] Soak wild-type (WT) Arabidopsis seeds in 75% alcohol for 5 minutes, then move to 50% Dettol and soak for 10 minutes, then wash the seeds 5 times with sterile water. The sterilized Arabidopsis seeds are spread in a culture dish containing three solid culture media, culture medium ①: 1 / 2MS basal culture medium, the pH of the culture medium is 5.7; culture medium ②: 1 / 2MS basal culture medium, pH 5.7, also includes: 100mmol / L NaCl; culture medium ③: 1 / 2MS basal culture medium, pH 5.7, also includes: 100mmol / L NaCl, 1mmol / L D-ribose. Seal the culture dish with sealing film to maintain sterility and appropriate humidity. Place the culture dish in a dark place at 4°C for vernalization for 3 days. After the vernalization treatment, move the culture dish to the Arabidopsis growth room, place it vertically, and culture it under long-day conditions at 22°C (16h light / 8h dark).

[0028] Example 2

[0029] The WT Arabidopsis plants grown on the culture medium ① for 7 days were transferred to soil for 7 days. The WT Arabidopsis plants were then divided into groups and cultured as shown in Table 1, and each group was cultured for 60 days.

[0030] Table 1

[0031]

[0032]

[0033] Example 3

[0034] The cotton seeds of Zhongmian 619 were germinated, then planted in the soil, and watered normally until the cotton grew true leaves. After the cotton grew true leaves, the cotton plants were grouped and cultured as shown in Table 2, and each group was cultured for 14 days.

[0035] Table 2

[0036]

[0037] Example 4

[0038] The B104 corn seeds were germinated and then planted in the soil until the corn grew true leaves. After the corn grew true leaves, the corn plants were grouped and cultured as shown in Table 3, and each group was cultured for 14 days.

[0039] Table 3

[0040]

[0041] Experimental example

[0042] Experimental Example 1

[0043] The phenotypes of the Arabidopsis plants obtained after culturing in the three culture media for 7 days in Example 1 were photographed and observed, and the changes in their growth and development were analyzed. The results are shown in Figure 1 shown.

[0044] Figure 1 The following is a phenotype diagram of Arabidopsis plants after culturing for 7 days using the three culture media in Example 1. Figure 1 It can be seen that compared with medium ①, culturing Arabidopsis plants with medium ② will inhibit the growth of the main root of Arabidopsis. Compared with medium ②, culturing Arabidopsis plants with medium ③ can effectively alleviate the inhibitory effect of salt stress on the growth of the main root of Arabidopsis. The above results show that the addition of D-ribose can effectively alleviate the inhibitory effect of salt stress on the growth of the main root of Arabidopsis.

[0045] Experimental Example 2

[0046] The phenotypes of the Arabidopsis plants in each group after culturing for 10 days and 60 days in Example 2 were photographed and observed, and the changes in their growth and development were analyzed. The results are shown in Figure 2 shown.

[0047] Figure 2 2 is a phenotypic diagram of each group of Arabidopsis plants after being cultured for different time periods in Example 2. Figure 2 A is the phenotype of each group of Arabidopsis plants after 10 days of cultivation in Example 2. Figure 2A shows that compared with the Control group, the leaf growth of Arabidopsis plants was inhibited under salt stress, and as the salt stress increased, the inhibitory effect on the leaf growth of Arabidopsis plants also increased. Compared with various salt concentration stress groups, the addition of D-ribose effectively alleviated the inhibitory effect of salt stress on the growth of Arabidopsis leaves. Figure 2 B is the phenotype of each group of Arabidopsis plants after 60 days of cultivation in Example 2. Figure 2 B shows that compared with the Control group, under salt stress, the plant height and leaf growth of Arabidopsis thaliana grown for 60 days were inhibited, and flowering and seed growth were severely inhibited. And with the increase of salt stress, the inhibitory effect on the plant height and leaf growth of Arabidopsis thaliana also increased. Compared with various salt concentration stress groups, the group with added D-ribose effectively alleviated the inhibitory effect of salt stress on the whole plant growth of Arabidopsis thaliana in the later stage. The above results show that the addition of D-ribose can effectively alleviate the inhibitory effect of salt stress on the plant height and leaf growth of Arabidopsis thaliana, and has a protective effect on the flowering and seed production of Arabidopsis thaliana in the later stage.

[0048] Experimental Example 3

[0049] After 14 days of cultivation, the phenotypes of the cotton plants in each group of Example 3 were photographed and observed, and the changes in their growth and development were analyzed. The results are shown in Figure 3 shown.

[0050] Figure 3 The phenotypes of cotton plants in each group after 14 days of cultivation in Example 3 are shown in FIG. Figure 3 It can be seen that compared with the Control group, under salt stress, the plant height and leaf growth of cotton were inhibited, and with the increase of salt stress, the inhibitory effect on cotton plant height and leaf growth also increased. Compared with various salt concentration stress groups, the addition of D-ribose effectively alleviated the inhibitory effect of salt stress on cotton plant height and leaf growth. The above results show that the addition of D-ribose can effectively alleviate the inhibitory effect of salt stress on cotton leaf growth.

[0051] Experimental Example 4

[0052] After 14 days of cultivation, the phenotypes of the corn plants in each group of Example 4 were photographed and observed, and the changes in their growth and development were analyzed. The results are shown in Figure 4 shown.

[0053] Figure 4 The phenotypes of corn plants in each group after 14 days of cultivation in Example 4 are shown in FIG. Figure 4It can be seen that compared with the Control group, under salt stress, the plant height and leaf growth of corn were inhibited, and with the increase of salt stress, the inhibitory effect on the plant height and leaf growth of corn also increased. Compared with various salt concentration stress groups, the groups with the addition of D-ribose effectively alleviated the inhibitory effect of salt stress on the plant height and leaf growth of corn. The above results show that the addition of D-ribose can effectively alleviate the inhibitory effect of salt stress on the plant height and leaf growth of corn. In summary, salt stress seriously damages the normal growth and development of the main root, plant height and leaves of plants, inhibits the overall growth of plants, and further affects the later flowering and seed formation, and this inhibitory effect intensifies with the deepening of salt stress. The addition of D-ribose can actively promote the growth of the main root, plant height and leaves of crops, effectively reduce the negative effects of salt stress, and at the same time ensure the smooth progress of later flowering and seed production.

[0054] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit them. The basic principles and main features of the present invention have been described above with specific implementation schemes. On the basis of the present invention, some modifications or replacements may be made thereto, but these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of protection claimed by the present invention.

Claims

1. Application of D-ribose in improving plant resistance to salt stress.

2. The use of D-ribose in improving plant resistance to salt stress according to claim 1, characterized in that: The concentration of the salt stress is 50-200 mmol / L.

3. The use of D-ribose in improving plant resistance to salt stress according to claim 1, characterized in that: The specific application method is: D-ribose is applied to the seeds or seedlings of the plant.

4. The use of D-ribose in improving plant resistance to salt stress according to claim 3, characterized in that: The administration concentration of the D-ribose is 0.5-1.5 mmol / L.

5. The use of D-ribose in improving plant resistance to salt stress according to claim 4, characterized in that: The administration concentration of the D-ribose was 1 mmol / L.

6. The use of D-ribose in improving plant resistance to salt stress according to claim 3, characterized in that: The specific method of applying D-ribose to plant seeds is: sterilizing the plant seeds and then sowing them in a culture medium containing D-ribose for cultivation.

7. The use of D-ribose in improving plant resistance to salt stress according to claim 3, characterized in that: The specific method of applying D-ribose to plant seedlings is: adding D-ribose to water or culture medium, and then spraying the leaves of the plant seedlings.

8. The use of D-ribose in improving plant resistance to salt stress according to claim 7, characterized in that: The plants are Arabidopsis, cotton, corn, wheat and soybean.

9. The use of D-ribose in improving plant resistance to salt stress according to claim 7, characterized in that: The frequency of spraying is once every 5 days; the amount of spraying is based on completely wetting all leaves.

10. The use of D-ribose in improving plant resistance to salt stress according to claim 1, characterized in that: The D-ribose can promote the growth of the main root, plant height and leaves of the plant, and effectively alleviate the inhibitory effect of salt stress on the growth and development of the plant.

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