Growth regulator for regulating fruit setting rate of fruit trees and preparation method thereof

Abstract

The invention relates to a growth regulator for regulating the fruit setting rate of fruit trees and a preparation method thereof, and belongs to the technical field of plant growth regulators. The growth regulator disclosed by the invention is mainly prepared from the following components in parts by weight: 0.5 to 0.8 part of a microbial fermentation product, 1 to 1.5 parts of boric acid, 3 to 3.5 parts of monopotassium phosphate, 3 to 5 parts of sodium alginate and 0.1 to 0.15 part of trimethylglycine. By using the growth regulator provided by the invention, the flower and fruit dropping rate of fruit trees can be reduced, the fruit setting rate and yield of the fruit trees can be effectively improved, and the fruit quality can also be improved.

Description

Technical Field

[0001] The invention belongs to the technical field of plant growth regulators and relates to a growth regulator for regulating the fruit setting rate of fruit trees and a preparation method thereof. Background Art

[0002] The natural fruit setting rate is also called the natural fruiting rate, which is the percentage of the actual fruit number of fruit trees in the natural state to the total number of flowers. For farmers, a low fruit setting rate directly affects the economic benefits of planting fruit trees. The main reasons for the fruit setting rate are as follows: 1. The soil is compacted, the root growth is hindered, the soil has not been plowed for many years, the organic matter content is low, which is not conducive to the expansion of the root system, resulting in poor root absorption capacity, insufficient utilization of nutrients in the soil, and inhibition of fruit setting; 2. Lack of fertilizer and water. Due to the strong adaptability of some fruit trees and extensive management, the trees are mostly left to grow, resulting in poor nutritional conditions for the trees, affecting fruit setting; 3. Nutritional imbalance. Some fruit trees bloom late, generally in parallel with the growth of new leaves, resulting in a large amount of nutrients used for branch and leaf construction, and relatively less nutrients for flower and fruit growth, resulting in serious flower and fruit falling; 4. High temperature causes closed flowers. Generally, when the temperature is too high, flowers are prone to closed flowers. If the flower blooming period coincides with the high temperature season, it is not conducive to pollination and fertilization, and finally the flowers and fruits fall off; 5. Low temperature causes scorched flowers. If the humidity is low and the temperature is high during the flowering period, scorched flowers will quickly appear, resulting in flowers but no fruits. In addition, pests and diseases, improper fertilization, and improper tree control can also cause varying degrees of flower and fruit drop. Summary of the invention

[0003] The main purpose of the present invention is to provide a growth regulator, which can significantly increase the fruit setting rate of fruit trees and improve the yield of fruit trees.

[0004] The present invention adopts the following technical solutions to achieve the above purpose: A growth regulator for regulating the fruit setting rate of fruit trees, mainly prepared from the following ingredients: 0.5-0.8 parts by weight of microbial fermentation product, 1-1.5 parts by weight of boric acid, 3-3.5 parts by weight of potassium dihydrogen phosphate, 3-5 parts by weight of sodium alginate, and 0.1-0.15 parts by weight of trimethylglycine.

[0005] Furthermore, the method for preparing the microbial fermentation product comprises the following steps: Step 1, inoculating the seed liquid of Gibberella fujikura and the seed liquid of Fusarium pyrifos into a fermentation medium containing defatted soybean meal, modified starch, sucrose, magnesium sulfate heptahydrate, zinc chloride, and potassium dihydrogen phosphate, and culturing in a shaking flask at 25° C. and 150 r / min for 24 hours, adding sunflower oil, stigmasterol, and citric acid to the culture medium, heating to 30° C. and continuing to culturing in the shaking flask for 48 hours, centrifuging after the culturing is completed, and using the supernatant I and the precipitate for later use; Step 2, adding water to the filter residue obtained in step 1 at a solid-liquid ratio of 1:8 kg / L, then adding peptone and L-tryptophan, mixing evenly, inoculating Bacillus Velez seed liquid and Bacillus megaterium seed liquid, culturing in a shaking flask at 30° C. and 150 r / min for 36 hours, centrifuging after the cultivation is completed, and using supernatant II for standby use; Step 3, combining the supernatant I and supernatant II obtained above, concentrating and then drying to obtain the microbial fermentation product.

[0006] Furthermore, in the above step 1, the number of effective live bacteria in the seed solution of Gibberella fujikura is 40 million cfu / mL, and the number of effective live bacteria in the seed solution of Fusarium erythrorhizium is 20 million cfu / mL.

[0007] Furthermore, in the above step 1, the inoculation amount of the seed solution of Gibberella fujikura is 10%, and the inoculation amount of the seed solution of Fusarium erythrorhizium is 4%.

[0008] Furthermore, in the above step 1, the fermentation medium contains 5-6 g / L defatted soybean meal, 35-40 g / L modified starch, 8-10 g / L sucrose, 0.15-0.2 g / L magnesium sulfate heptahydrate, 0.08-0.1 g / L zinc chloride, and 1.5-2 g / L potassium dihydrogen phosphate.

[0009] Furthermore, in the above step 1, the amount of sunflower oil added is 3-5 g / L, the amount of stigmasterol added is 3-5 g / L, and the amount of citric acid added is 1-1.5 g / L.

[0010] Furthermore, in the above step 2, the amount of peptone added is 13-15 g / L, and the amount of L-tryptophan added is 0.3-0.5 g / L.

[0011] Furthermore, in the above step 2, the inoculation amount of the Bacillus velez seed solution is 8%, and the inoculation amount of the Bacillus megaterium seed solution is 4%.

[0012] Furthermore, in the above step 2, the effective viable bacteria count in the Bacillus velez seed solution is 100 million cfu / mL, and the effective viable bacteria count in the Bacillus megaterium seed solution is 50 million cfu / mL.

[0013] The present invention provides a method for preparing the modified starch in the above step 1, which mainly comprises the following steps: Step A, adding water to starch and mixing, heating and stirring until viscous, and then cooling to room temperature to obtain gelatinized starch; Step B, adding a complex enzyme aqueous solution containing β-amylase and maltogenic amylase to the gelatinized starch, heating to 40° C., and maintaining the temperature for enzymolysis for 40-60 minutes. After the enzymolysis is completed, the enzyme is inactivated and the enzymolysis solution is used for standby use; Step C, adding NaMoO to the enzymatic solution 4 ·2H2 O, after mixing, heat and stir until viscous state, cool, dry and crush to obtain modified starch.

[0014] Furthermore, in the above step B, the solid-liquid ratio of gelatinized starch to complex enzyme aqueous solution is 1:(0.8-1) kg / L.

[0015] Furthermore, the aqueous solution of the complex enzyme in the above step B contains 3-3.5 g / L of β-amylase and 2-3 g / L of maltogenic amylase.

[0016] Furthermore, in the above step C, NaMoO 4 ·2H 2 The amount of O added is 0.1-0.15% of the mass of gelatinized starch.

[0017] The present invention provides a method for preparing the growth regulator, comprising the following steps: Dissolve sodium alginate in water, add microbial fermentation products, mix well, dry and crush; then add boric acid, potassium dihydrogen phosphate and trimethylglycine and mix well to obtain a growth regulator.

[0018] The present invention has the following beneficial effects: Firstly, the present invention adopts a variety of microbial fermentation methods to obtain a fermentation product containing plant hormones such as gibberellins and indoleacetic acid, and then compound other ingredients to obtain a growth regulator, which can significantly increase the fruit setting rate of fruit trees and improve the fruit quality.

[0019] Secondly, in the process of preparing microbial fermentation products, the culture medium containing modified starch is first used to culture Gibberella fujikura and Fusarium spp., and then sunflower oil, stigmasterol and citric acid are used as supplements to induce and stimulate the microorganisms to produce metabolites such as gibberellins, and the fermentation liquid after culture contains various metabolites including gibberellins; based on the residue of the culture medium, Bacillus velez, Bacillus megaterium and the like are inoculated after adding materials such as L-tryptophan, and in the process of culture, metabolites including indoleacetic acid are produced during the proliferation and growth of Bacillus, and the finally prepared microbial fermentation products contain various microbial metabolites including gibberellins and indoleacetic acid, which can regulate fruit trees, effectively solve the problems of low fruit setting rate of fruit trees caused by incomplete development of flower buds and high temperature of greenhouse cultivation, significantly improve the fruit setting rate of fruit trees, and also improve the quality of fruits, with remarkable effects. DETAILED DESCRIPTION

[0020] The present invention is further explained below in conjunction with specific examples. It should be understood that these examples are only used to illustrate the present invention and are not used to limit the scope of the present invention. After reading the present invention, various equivalent modifications to the present invention by those skilled in the art all fall within the scope of protection of the claims of this application.

[0021] Example 1 1. Preparation of modified starch: Step A, adding water to starch and mixing, heating and stirring until viscous, and then cooling to room temperature to obtain gelatinized starch; Step B, taking 1 kg of gelatinized starch, adding 0.8 L of a complex enzyme aqueous solution containing 3.5 g / L of β-amylase and 2 g / L of maltogenic amylase, heating to 40° C., and keeping the temperature for enzymolysis for 60 min. After the enzymolysis is completed, the enzyme is inactivated and the enzymolysis solution is used for standby use; Step C, adding NaMoO to the enzymatic solution 4 ·2H 2 O 1g, mix well, heat and stir until viscous state, cool, dry and crush to obtain modified starch.

[0022] 2. Preparation of microbial fermentation products: Step 1, inoculating a seed solution of Fusarium fusogenum with an effective viable count of 40 million cfu / mL and a seed solution of Fusarium sphaeroides with an effective viable count of 20 million cfu / mL into a fermentation medium containing 6 g / L defatted soybean meal, 35 g / L modified starch, 10 g / L sucrose, 0.15 g / L magnesium sulfate heptahydrate, 0.1 g / L zinc chloride, and 1.5 g / L potassium dihydrogen phosphate at an inoculation rate of 10% and 8%, respectively, and culturing in a shake flask at 25° C. and 150 r / min for 24 hours, adding 5 g / L sunflower oil, 3 g / L stigmasterol, and 1.5 g / L citric acid to the culture medium, heating to 30° C. and continuing to shake the flask for 48 hours, centrifuging after the culturing is completed, and using the supernatant I and the precipitate for later use; Step 2, add water to the filter residue obtained in step 1 at a solid-liquid ratio of 1:8 kg / L, then add 15 g / L of peptone and 0.3 g / L of L-tryptophan, mix well, inoculate with 8% and 4% of the inoculation amount of Bacillus Velezii seed solution with an effective viable count of 100 million cfu / mL and Bacillus megaterium seed solution with an effective viable count of 50 million cfu / mL, respectively, and culture at 30° C. and 150 r / min for 36 hours. After the culture is completed, centrifuge and use the supernatant II for later use; Step 3, combining the supernatant I and supernatant II obtained above, concentrating and then drying to obtain the microbial fermentation product.

[0023] 3. Preparation of growth regulators: Dissolve 5 kg of sodium alginate in water, add 0.8 kg of microbial fermentation product, mix well, dry and crush; then add 1 kg of boric acid, 3.5 kg of potassium dihydrogen phosphate and 0.1 kg of trimethylglycine and mix well to obtain a growth regulator.

[0024] Example 2 1. Preparation of modified starch: Step A, adding water to starch and mixing, heating and stirring until viscous, and then cooling to room temperature to obtain gelatinized starch; Step B, taking 1 kg of gelatinized starch, adding 1 L of a complex enzyme aqueous solution containing 3 g / L of β-amylase and 3 g / L of maltogenic amylase, heating to 40°C, and keeping the temperature for enzymolysis for 40 minutes. After the enzymolysis is completed, the enzyme is inactivated and the enzymolysis solution is used for standby use; Step C, adding NaMoO to the enzymatic solution 4 ·2H 2 O 1.5g, mix well, heat and stir until viscous, cool, dry and crush to obtain modified starch.

[0025] 2. Preparation of microbial fermentation products: Step 1, inoculating a Fujikura Gibberella seed solution with an effective viable count of 400 million cfu / mL and a Fusarium sphaeroides seed solution with an effective viable count of 200 million cfu / mL into a fermentation medium containing 5 g / L defatted soybean meal, 40 g / L modified starch, 8 g / L sucrose, 0.2 g / L magnesium sulfate heptahydrate, 0.08 g / L zinc chloride, and 2 g / L potassium dihydrogen phosphate at an inoculation rate of 10% and 8%, respectively, and culturing in a shake flask at 25° C. and 150 r / min for 24 hours, adding 3 g / L sunflower oil, 5 g / L stigmasterol, and 1 g / L citric acid to the culture medium, heating to 30° C. and continuing to shake the flask for 48 hours, centrifuging after the culturing is completed, and using the supernatant I and the precipitate for later use; Step 2, adding water to the filter residue obtained in step 1 at a solid-liquid ratio of 1:8 kg / L, then adding 13 g / L of peptone and 0.5 g / L of L-tryptophan, mixing evenly, inoculating with 8% and 4% of the inoculation amount of Bacillus Velezii seed solution with an effective viable count of 100 million cfu / mL and Bacillus megaterium seed solution with an effective viable count of 50 million cfu / mL, respectively, culturing in a flask at 30° C. and 150 r / min for 36 hours, centrifuging after the culturing is completed, and using the supernatant II for later use; Step 3, combining the supernatant I and supernatant II obtained above, concentrating and then drying to obtain the microbial fermentation product.

[0026] 3. Preparation of growth regulators: Dissolve 3 kg of sodium alginate in water, then add 0.5 kg of microbial fermentation product, mix well, dry and crush; then add 1.5 kg of boric acid, 3 kg of potassium dihydrogen phosphate and 0.15 kg of trimethylglycine and mix well to obtain a growth regulator.

[0027] Example 3 1. Preparation of modified starch: Step A, adding water to starch and mixing, heating and stirring until viscous, and then cooling to room temperature to obtain gelatinized starch; Step B, taking 1 kg of gelatinized starch, adding 1 L of a complex enzyme aqueous solution containing 3.3 g / L of β-amylase and 2.5 g / L of maltogenic amylase, heating to 40°C, and keeping the temperature for enzymolysis for 50 minutes. After the enzymolysis is completed, the enzyme is inactivated and the enzymolysis solution is used for standby use; Step C, adding NaMoO to the enzymatic solution 4 ·2H 2 O 1.3g, mix well, heat and stir until viscous, cool, dry and crush to obtain modified starch.

[0028] 2. Preparation of microbial fermentation products: Step 1, inoculating a Fujikura Gibberella seed solution with an effective viable count of 40 million cfu / mL and a Fusarium sphaeroides seed solution with an effective viable count of 20 million cfu / mL into a fermentation medium containing 5 g / L defatted soybean meal, 38 g / L modified starch, 9 g / L sucrose, 0.18 g / L magnesium sulfate heptahydrate, 0.09 g / L zinc chloride, and 1.8 g / L potassium dihydrogen phosphate at an inoculation rate of 10% and 8%, respectively, and culturing in a shake flask at 25° C. and 150 r / min for 24 hours, adding 4 g / L sunflower oil, 4 g / L stigmasterol, and 1.2 g / L citric acid to the culture medium, heating to 30° C. and continuing to shake the flask for 48 hours, centrifuging after the culture is completed, and using the supernatant I and the precipitate for later use; Step 2, add water to the filter residue obtained in step 1 at a solid-liquid ratio of 1:8 kg / L, then add 14 g / L of peptone and 0.4 g / L of L-tryptophan, mix well, inoculate with 8% and 4% of the inoculation amount of Bacillus Velezii seed solution with an effective viable count of 100 million cfu / mL and Bacillus megaterium seed solution with an effective viable count of 50 million cfu / mL, respectively, shake culture at 30° C. and 150 r / min for 36 hours, centrifuge after the culture is completed, and use the supernatant II for later use; Step 3, combining the supernatant I and supernatant II obtained above, concentrating and then drying to obtain the microbial fermentation product.

[0029] 3. Preparation of growth regulators: Dissolve 4 kg of sodium alginate in water, then add 0.6 kg of microbial fermentation product, mix well, dry and crush; then add 1.3 kg of boric acid, 3.3 kg of potassium dihydrogen phosphate and 0.13 kg of trimethylglycine and mix well to obtain a growth regulator.

[0030] Comparative Example 1 1. Preparation of microbial fermentation products: Step 1, inoculating a seed solution of Fusarium fujikura with an effective viable count of 400 million cfu / mL and a seed solution of Fusarium sphaeroides with an effective viable count of 200 million cfu / mL into a fermentation medium containing 5 g / L defatted soybean meal, 38 g / L starch, 9 g / L sucrose, 0.18 g / L magnesium sulfate heptahydrate, 0.09 g / L zinc chloride, and 1.8 g / L potassium dihydrogen phosphate at an inoculation rate of 10% and 8%, respectively, and culturing in a shake flask at 25° C. and 150 r / min for 48 hours. After the culturing is completed, centrifugation is performed, and the supernatant I and the precipitate are reserved; Step 2, add water to the filter residue obtained in step 1 at a solid-liquid ratio of 1:8 kg / L, then add 14 g / L of peptone and 0.4 g / L of L-tryptophan, mix well, inoculate with 8% and 4% of the inoculation amount of Bacillus Velezii seed solution with an effective viable count of 100 million cfu / mL and Bacillus megaterium seed solution with an effective viable count of 50 million cfu / mL, respectively, shake culture at 30° C. and 150 r / min for 36 hours, centrifuge after the culture is completed, and use the supernatant II for later use; Step 3, combining the supernatant I and supernatant II obtained above, concentrating and then drying to obtain the microbial fermentation product.

[0031] 3. Preparation of growth regulators: Dissolve 4 kg of sodium alginate in water, then add 0.6 kg of microbial fermentation product, mix well, dry and crush; then add 1.3 kg of boric acid, 3.3 kg of potassium dihydrogen phosphate and 0.13 kg of trimethylglycine and mix well to obtain a growth regulator.

[0032] Comparative Example 2 1. Preparation of modified starch: Step A, adding water to starch and mixing, heating and stirring until viscous, and then cooling to room temperature to obtain gelatinized starch; Step B, taking 1 kg of gelatinized starch, adding 1 L of a complex enzyme aqueous solution containing 3.3 g / L of β-amylase and 2.5 g / L of maltogenic amylase, heating to 40°C, and keeping the temperature for enzymolysis for 50 minutes. After the enzymolysis is completed, the enzyme is inactivated and the enzymolysis solution is used for standby use; Step C, heating and stirring until viscous state, cooling, drying and crushing to obtain modified starch.

[0033] 2. Preparation of microbial fermentation products: Step 1, inoculate the seed solution of Fusarium fusogenum with an effective viable count of 400 million cfu / mL and the seed solution of Fusarium sphaeroides with an effective viable count of 200 million cfu / mL into a fermentation medium containing 5 g / L defatted soybean meal, 38 g / L modified starch, 9 g / L sucrose, 0.18 g / L magnesium sulfate heptahydrate, 0.09 g / L zinc chloride, 1.8 g / L potassium dihydrogen phosphate, 4 g / L sunflower oil, 4 g / L stigmasterol, and 1.2 g / L citric acid at an inoculation rate of 10% and 8%, respectively. In the fermentation medium, shake the flask for 48 hours at 25°C and 150 r / min, then add 14 g / L peptone and 0.4 g / L L-tryptophan, mix well, inoculate 8% and 4% of the inoculation amount of Bacillus Velezii seed solution with an effective viable count of 100 million cfu / mL and Bacillus megaterium seed solution with an effective viable count of 50 million cfu / mL, respectively, shake the flask for 36 hours at 30°C and 150 r / min, centrifuge after the culture is completed, and use the supernatant for later use; Step 3, concentrating and drying the obtained supernatant to obtain the microbial fermentation product.

[0034] 3. Preparation of growth regulators: Dissolve 4 kg of sodium alginate in water, add 0.6 kg of microbial fermentation product, mix well, dry and crush; then add 1.3 kg of boric acid and 3.3 kg of potassium dihydrogen phosphate and mix well to obtain a growth regulator.

[0035] Comparative Example 3 Dissolve 4 kg of sodium alginate in water, add 6 g of gibberellin and 10 g of indoleacetic acid, mix well, dry and crush; then add 1.3 kg of boric acid, 3.3 kg of potassium dihydrogen phosphate and 0.13 kg of trimethylglycine and mix well to obtain a growth regulator.

[0036] Performance Testing 1. Effect of the regulator of the present invention on the fruit setting rate of greenhouse blueberries 1. Experimental materials and methods The blueberry variety selected in this experiment is "Rika", which is about 5 years old. The experiment was carried out in a greenhouse in a blueberry planting base.

[0037] In this experiment, blueberry plants with basically the same growth, strong growth and no pests and diseases were selected as test plants. The blueberries were randomly divided into 8 groups, each with 10 plants, namely RK-KB group, RK-DZ group, RK-S1 group-RK-S3 group, and RK-D1 group-RK-D3 group. Each group was treated in the following way during the flowering period: RK-KB group: spray with clean water; RK-DZ group: spray commercially available 14-carboxybrassinosteroid aqueous solution (active ingredient content 0.0075%), diluted according to the dosage in the instructions for use; RK-S1 group: sprayed with the growth regulator of Example 1 (50 mg / L); RK-S2 group: sprayed with the growth regulator of Example 2 (50 mg / L); RK-S3 group: sprayed with the growth regulator of Example 3 (50 mg / L); RK-D1 group: sprayed with the growth regulator of comparative example 1 (50 mg / L); RK-D2 group: sprayed with the growth regulator of comparative example 2 (50 mg / L); RK-D3 group: sprayed with the growth regulator of comparative example 3 (50 mg / L); The spraying method was to spray the whole plant until the plant was completely moistened and dripping, and the rest was conventional field management. One branch was randomly selected from each group for marking, and the fruit setting rate and fruit appearance quality were measured.

[0038] Five blueberry plants were randomly selected from each group, and 10 fruits were randomly selected from each plant. The vertical and horizontal diameters of the fruits were measured with a vernier caliper, and the fruit shape index was calculated and the average value was taken. After the total weight was weighed on a balance, the average weight of a single fruit was calculated. The fruit hardness was measured with a fruit hardness meter and the average value was taken.

[0039] 2. Test results and analysis As shown in the results in Table 1 below, the use of the regulator provided by the present invention can significantly increase the blueberry fruit setting rate, improve the blueberry fruit shape index, the blueberry fruit single fruit weight is higher, the fruit hardness is larger, and the overall blueberry fruit yield and quality are better, and its effect is equivalent to or better than the traditional brassinolide regulator. The data of each group are compared using SPSS22.0 data processing software, and there is a significant difference between the blueberry data using the regulator of the present invention and the blueberry data using the regulators of Comparative Examples 1-Comparative Examples 3, which is statistically significant.

[0040] Table 1 Fruit setting rate and fruit quality of blueberry after different treatments 2. Effect of the Growth Regulator of the Present Invention on Fruit Setting Rate of Greenhouse Cherries 1. Experimental materials and methods This experiment was conducted in a cherry greenhouse in a village. The "red light" cherry trees with an age of 8 years were selected for this experiment. The cherry trees were randomly divided into 8 groups, with 5 trees in each group, namely HD-KB group, HD-DZ group, HD-S1 group to HD-S3 group, HD-D1 group to HD-D3 group. Each group was treated in the following manner during the blooming period and 10 days after the flowers fell: HD-KB group: spray with clean water; HD-DZ group: spray commercially available benzylamine gibberellic acid (total active ingredient content 3.6%), diluted according to the dosage in the instructions for use; HD-S1 group: sprayed with the growth regulator of Example 1 (100 mg / L); HD-S2 group: sprayed with the growth regulator of Example 2 (100 mg / L); HD-S3 group: sprayed with the growth regulator of Example 3 (100 mg / L); HD-D1 group: sprayed with the growth regulator of comparative example 1 (100 mg / L); HD-D2 group: sprayed with the growth regulator of comparative example 2 (100 mg / L); HD-D3 group: sprayed with the growth regulator of comparative example 3 (100 mg / L); The spraying method is to spray the whole plant until the plant is completely moistened and dripping, and the rest is conventional field management. Before the treatment of each group of cherries in the blooming period, three main branches with basically the same growth were selected for each plant, the number of flowers was recorded and marked, and the number of fruits was recorded after physiological fruit drop, and the fruit setting rate was calculated; 10 fruits were randomly selected from each main branch, and the average single fruit weight was calculated after weighing.

[0041] 2. Test results and analysis As can be seen from the results in Table 2 below, the regulator of the present invention can effectively improve the fruit setting rate of cherries and increase the single fruit weight, and the effect is better than using the regulators of Comparative Examples 1 to 3. After data statistics and comparison, there are significant differences.

[0042] Table 2 Fruit setting rate and single fruit weight of cherry after different treatments 3. Effect of the Growth Regulator of the Present Invention on Apple Fruit Setting Rate 1. Experimental materials and methods This experiment was conducted in an apple orchard in a village. The selected apple trees were 8-year-old "Guoguang" apple trees with good growth. The apple trees were randomly divided into 8 groups, with 2 trees in each group, namely GG-KB group, GG-DZ group, GG-S1 group to GG-S3 group, GG-D1 group to GG-D3 group. Each group was sprayed for the first time at the end of the flowering period (lightly shaking the branches and petals falling), and then sprayed for the second time after an interval of 10 days. The rest were conventional field management (fertilization, fruit thinning, etc.).

[0043] GG-KB group: spray with clean water; GG-DZ group: spray commercially available 14-carboxybrassinosteroid aqueous solution (active ingredient content 0.0075%), diluted according to the dosage in the instructions for use; Group GG-S1: sprayed with the growth regulator of Example 1 (150 mg / L); GG-S2 group: sprayed with the growth regulator of Example 2 (150 mg / L); GG-S3 group: sprayed with the growth regulator of Example 3 (150 mg / L); GG-D1 group: sprayed with the growth regulator of comparative example 1 (150 mg / L); GG-D2 group: sprayed with the growth regulator of comparative example 2 (150 mg / L); GG-D3 group: sprayed with the growth regulator of comparative example 3 (150 mg / L); Before spraying, four branches were randomly selected from the east, west, south and north directions of each apple tree in each group, and the number of inflorescences was investigated and marked. After physiological fruit drop, the number of fruit-bearing inflorescences on the marked branches was investigated and the inflorescence fruit setting rate was calculated.

[0044] Two fruits were randomly selected from each branch, their transverse diameter and longitudinal diameter were measured, and the fruit shape index and average single fruit weight were calculated.

[0045] 2. Test results and analysis As can be seen from the results in Table 3 below, the regulator of the present invention can effectively improve the fruit setting rate and fruit quality of apples, and the effect is better than the regulators obtained in Comparative Examples 1 to 3.

[0046] Table 3 Fruit setting rate of apples after different treatments From the above test results, it can be seen that the regulator provided by the present invention can significantly improve the fruit setting rate of blueberry, cherry, apple and other fruit trees. The preparation method of the regulator in Comparative Example 1-Comparative Example 2 is significantly different from that of the present invention, resulting in different components in the regulator and different regulatory effects on fruit trees. Although the fruit setting rate of fruit trees can be improved to a certain extent, the effect is not as good as the regulator of the present invention. Conventional plant hormones gibberellin and indoleacetic acid are added to the regulator in Comparative Example 3, and the effect is poor compared with the regulator of the present invention, indicating that the active ingredients in the microbial fermentation product in the present invention play an important role in regulating the fruit setting rate of fruit trees.

Claims

1. A growth regulator for regulating the fruit setting rate of fruit trees, characterized in that: Prepared from the following ingredients: 0.5-0.8 parts by weight of microbial fermentation product, 1-1.5 parts by weight of boric acid, 3-3.5 parts by weight of potassium dihydrogen phosphate, 3-5 parts by weight of sodium alginate, and 0.1-0.15 parts by weight of trimethylglycine.

2. The growth regulator according to claim 1, characterized in that The method for preparing the microbial fermentation product comprises the following steps: Step 1, inoculating the seed liquid of Gibberella fujikura and the seed liquid of Fusarium pyrifos into a fermentation medium containing defatted soybean meal, modified starch, sucrose, magnesium sulfate heptahydrate, zinc chloride, and potassium dihydrogen phosphate, and culturing in a shaking flask at 25° C. and 150 r / min for 24 hours, adding sunflower oil, stigmasterol, and citric acid to the culture medium, heating the medium to 30° C. and continuing to culturing in the shaking flask for 48 hours, centrifuging after the culturing is completed, and using the supernatant I and the precipitate for later use; Step 2, adding water to the filter residue obtained in step 1 at a solid-liquid ratio of 1:8 kg / L, then adding peptone and L-tryptophan, mixing evenly, inoculating Bacillus Velez seed liquid and Bacillus megaterium seed liquid, culturing in a shaking flask at 30° C. and 150 r / min for 36 hours, centrifuging after the cultivation is completed, and using supernatant II for standby use; Step 3, combining the supernatant I and supernatant II obtained above, concentrating and then drying to obtain the microbial fermentation product.

3. The growth regulator according to claim 2, characterized in that In the step 1, the inoculation amount of the seed solution of Gibberella fujikura is 10%, and the inoculation amount of the seed solution of Fusarium erythrorhizium is 4%.

4. The growth regulator according to claim 2, characterized in that The fermentation medium in step 1 contains 5-6 g / L defatted soybean meal, 35-40 g / L modified starch, 8-10 g / L sucrose, 0.15-0.2 g / L magnesium sulfate heptahydrate, 0.08-0.1 g / L zinc chloride, and 1.5-2 g / L potassium dihydrogen phosphate.

5. The growth regulator according to claim 2, characterized in that In the step 1, the amount of sunflower oil added is 3-5 g / L, the amount of stigmasterol added is 3-5 g / L, and the amount of citric acid added is 1-1.5 g / L; in the step 2, the amount of peptone added is 13-15 g / L, and the amount of L-tryptophan added is 0.3-0.5 g / L.

6. The growth regulator according to claim 2, characterized in that In the step 2, the inoculation amount of the Bacillus Velez seed solution is 8%, and the inoculation amount of the Bacillus megaterium seed solution is 4%.

7. The growth regulator according to claim 2, characterized in that The preparation method of modified starch in step 1 comprises the following steps: Step A, adding water to starch and mixing, heating and stirring until viscous, and then cooling to room temperature to obtain gelatinized starch; Step B, adding a complex enzyme aqueous solution containing β-amylase and maltogenic amylase to the gelatinized starch, heating to 40° C., and maintaining the temperature for enzymolysis for 40-60 minutes. After the enzymolysis is completed, the enzyme is inactivated and the enzymolysis solution is used for standby use; Step C, adding NaMoO4·2H2O to the enzymatic hydrolyzate, mixing, heating and stirring until viscous state, cooling, drying and crushing to obtain modified starch.

8. The growth regulator according to claim 7, characterized in that The solid-liquid ratio of gelatinized starch to complex enzyme aqueous solution in step B is 1:(0.8-1) kg / L; the complex enzyme aqueous solution contains 3-3.5 g / L of β-amylase and 2-3 g / L of maltogenic amylase.

9. The growth regulator according to claim 7, characterized in that In step C, the amount of NaMoO4·2H2O added is 0.1-0.15% of the mass of gelatinized starch.

10. A method for preparing a growth regulator according to any one of claims 1 to 9, characterized in that: The following steps are involved: Dissolve sodium alginate in water, add microbial fermentation products, mix well, dry and crush; then add boric acid, potassium dihydrogen phosphate and trimethylglycine and mix well to obtain a growth regulator.

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