Byproduct composition for promoting plant growth and reducing weeds and preparation method thereof

Abstract

The invention discloses a byproduct composition for promoting plant growth and reducing weeds and a preparation method thereof. The composition is prepared from the following raw materials in percentage by mass: 20 to 30 percent of camellia oleifera shells, 10 to 20 percent of camellia oleifera leaves, 10 to 30 percent of tea bran, 1 to 10 percent of pine byproducts, 1 to 10 percent of eucalyptus branches and leaves, 0.1 to 10 percent of eucalyptus oil and 0.1 to 10 percent of zymophyte combination. The preparation method comprises the following steps: crushing camellia oleifera shells, camellia oleifera leaves, tea bran, eucalyptus branches and leaves and pine byproducts, and mixing the raw materials with water to obtain a mixed solution; heating the mixed solution, and adding a zymophyte combination for mixed fermentation; and filtering the fermented mixed solution, adjusting the pH value, and adding eucalyptus oil to obtain the fertilizer. The raw materials are agricultural and forestry residues which are simple, convenient, easy to obtain, simple to operate and low in price, and the effects of improving soil, improving crop quality and reducing environmental pollution can be achieved when the fertilizer is applied to forest land.

Description

Technical Field

[0001] The invention belongs to the technical field of agricultural and forestry ecology, and particularly relates to a byproduct composition for promoting plant growth and reducing weeds and a preparation method thereof. Background Art

[0002] Agricultural and forestry residues are an important member of waste, an important biomass resource, and an important renewable resource. The energy conversion and utilization of agricultural and forestry residues is one of the research hotspots in the field of renewable energy. my country has rich solid biomass resources, with 150 million tons of by-products such as forest logging and wood processing each year. The energy potential of agricultural and forestry residues in my country is equivalent to 350 million tons of standard coal, which is approximately equivalent to 1 / 6 of the total energy consumption in my country. However, in my country, a large part of agricultural and forestry residues are directly burned or discarded, seriously polluting the environment. Camellia oleifera shells are a by-product of camellia oleifera fruit used to process tea oil. The annual production of camellia oleifera shells in China is about 1×10 10 kg. Camellia oleifera shells are rich in active substances such as lignin, pentosan, saponin and tannin, which can be used as bioenergy or applied in food and industrial fields. The organic matter content is about twice that of chicken manure. Camellia oleifera bran, as a by-product of tea oil processing, contains 12%-18% of plant-derived pesticide saponin, which has a good stomach poison and contact killing effect on pests. Tea bran can be mixed with compost and manure as base fertilizer, or used as topdressing or made into tea bran water for root irrigation of fruit trees and vegetables. As an organic fertilizer, the nutrients contained in tea bran, such as nitrogen, are mainly in the form of protein, and phosphorus is mainly in the form of lecithin. Therefore, it is easier for plants to absorb and utilize it after fermentation and decomposition. In addition, tea bran has a low nitrogen content and a high oil content. It can generate a lot of heat during fermentation, and it will cause root burn if it is not decomposed. Studies have shown that oil tea leaves contain a variety of active ingredients, such as polysaccharides, tea polyphenols, flavonoids and other antioxidants, which can not only promote plant growth, but also provide carbon sources for the fermentation process.

[0003] The whole pine tree is a treasure. Its trunk is a widely used wood and a high-quality papermaking raw material. The resin of the pine tree can be used to make rosin and turpentine. In the past, pine needles were basically left to rot or burn during the felling process, which not only wasted forest resources, but also easily caused forest fires, soil acidification, and pests and diseases. Although there is rosin in the bark of the pine tree, collecting rosin will come at a heavy cost with little benefit. A pine tree can only be harvested once a year. The pine tree with bark ring cut cannot be made into timber and will die within 8-10 years due to lack of nutrient sources. Therefore, destructive harvesting can only cause resource depletion and waste. The collection of pine needles is non-destructive collection (only 1 / 3-1 / 4 is collected once a season, or collected in pieces), which has no effect on the growth of the pine tree and can turn waste into treasure. Pine needles are rich in amino acids, a variety of fat-soluble and water-soluble vitamins, more than 40 kinds of macroelements and a variety of trace elements and crude fiber, and can be used as feed additives and organic fertilizers. Pine bark is often used as waste after collecting rosin. In recent years, pine bark has been successfully used as filter material in biofilters. Because pine bark has high organic matter content, high porosity, wide total interception area, good adsorption effect, stable pH value, long service life and other characteristics, it has good effects on waste gas filtration, water interception and carrying microbial populations in biofilters. The natural characteristics of pine bark - high organic content; suitable chemical composition, stable pH and low EC content, high N, P, K, Ca, Mg; stable physical properties, uniform and slow decomposition, achieving long-term fertilization; good air permeability and air and water control. This makes pine bark widely used in flower cultivation, crop planting and other fields. Therefore, using pine by-products as organic fertilizers has a promising market.

[0004] Eucalyptus planting in my country accounts for nearly 2.5% of the total forest area in the country, and is mainly used for papermaking, making artificial boards and extracting essential oils. Eucalyptus leaves contain a large amount of volatile secondary metabolites, such as eucalyptol, α-pinene, eucalyptol, limonene, ρ-paracymene, citronellal and eucalyptol, which have good insecticide, antibacterial, anti-inflammatory, analgesic, antioxidant and other biological activities, and have great economic value. However, in recent years, ecological problems such as soil degradation, loss of understory biodiversity, and loss of ecosystem functions caused by eucalyptus planting have gradually become social concerns. Note and academic debate hot issues, eucalyptus and eucalyptus volatile oil also have strong allelopathic effects on surrounding crops, mainly through volatilization and litter leaching solutions, especially leaf litter leaching solutions can produce a large number of allelopathic substances such as phenolic acid, esters, alkanes, etc., change soil pH, and significantly inhibit seed germination and plant growth. Therefore, how to collect eucalyptus leaves and deeply value-added processing and utilization, combined with other agricultural and forestry residues can not only save costs, but also reduce weeds by waste utilization, and promote the growth of eucalyptus and other crops is the main goal of the present invention. And every year in the process of shaping and pruning, low-yield forest transformation, etc., a large number of oil tea leaves are used as waste, causing great waste. At present, the processed oil tea shells and oil tea leaves are often not effectively utilized and are discarded or burned, which not only wastes resources, but also causes certain environmental pollution. The present invention uses agricultural and forestry residues such as oil tea shells, tea bran, oil tea leaves, eucalyptus, pine by-products, etc. as fertilizer raw materials, which can effectively improve soil fertility, and are cheap and easy to obtain, so that they can become fertilizers for flowers and seedlings.

[0005] Environmental pollution and weed resistance caused by long-term use of chemical herbicides are becoming increasingly prominent. Studies have found that plant essential oils contain a large amount of phenols, terpenes and organic acids, which have significant plant allelopathic effects. The use of plant essential oils to control weeds by allelopathic effects on plants has broad prospects. Eucalyptus oil contains 1,8-cineole, which has a strong allelopathic effect. Red eucalyptus essential oil has an inhibitory effect on the seed germination and growth of green spike amaranth and purslane. Lemon eucalyptus essential oil has a significant allelopathic effect on Xanthium sibiricum and wild oats, inhibiting seed germination and seedling growth. Eucalyptus regia essential oil has a significant inhibitory effect on ryegrass seed germination and seedling growth, and has an obvious dose effect. When the allelopathic substance reaches a certain concentration, it will produce an allelopathic effect on the plant. Therefore, the ratio of the present invention should not only promote plant growth, but also have a certain allelopathic effect to inhibit weeds. Camellia oleifera is a cross-pollinating plant that relies on insects such as bumblebees for pollination. Applying herbicides in the Camellia oleifera forest will not only kill the weeds, but also affect the development and behavior of the pollinating insects, which will directly affect the fruit setting and yield of Camellia oleifera. In addition, studies have shown that the application of glyphosate has a significant inhibitory effect on soil microorganisms, destroys the soil structure, and easily affects the growth environment of Camellia oleifera trees, thereby affecting the growth of Camellia oleifera trees. Therefore, try not to apply herbicides such as glyphosate.

[0006] Camellia oleifera shells are rich in cellulose and lignin. How to quickly ferment camellia oleifera shells is the key to processing and utilizing camellia oleifera shells. Studies have shown that camellia oleifera shells can be composted by inoculating composite microbial flora. Microbial fermentation can promote the decomposition of agricultural and forestry residues such as camellia oleifera shells, and produce a variety of metabolites beneficial to plants, such as auxin, gibberellin, vitamins, amino acids, etc., which can stimulate root growth, increase absorption area, and effectively regulate vegetative growth and reproductive growth, promote flower bud differentiation, and provide sufficient nutrient supply. There is no prior art that uses camellia oleifera shells, oil tea leaves, tea bran, eucalyptus branches and leaves, pine by-products, eucalyptus extracts and microorganisms to prepare a composition. Reasonable proportions and probiotic fermentation can better reduce the allelopathic effect of eucalyptus leaves, promote the growth of eucalyptus and other plants, and are used to prevent and control plant pests, improve soil, improve crop quality, and improve crop stress resistance, thereby achieving cost-saving, efficiency-enhancing, comprehensive utilization, and green environmental protection. Summary of the invention

[0007] In view of the above problems, the present invention provides a byproduct composition for promoting plant growth and reducing weeds and a preparation method thereof. The composition is prepared by using oil tea shells, oil tea leaves, tea bran, eucalyptus branches and leaves, pine byproducts, eucalyptus oil and microorganisms. The present invention can better promote the growth of eucalyptus and other plants, promote fruit setting, and effectively inhibit weeds. It is used to reduce plant diseases and insect pests, improve soil, improve crop quality, and enhance crop stress resistance, thereby achieving the effects of cost saving, efficiency improvement, comprehensive utilization, and green environmental protection.

[0008] In order to achieve the above technical objectives, the present invention adopts the following technical solutions:

[0009] A byproduct composition for promoting plant growth and reducing weeds comprises the following raw materials in percentage by mass: 20-30% of camellia oleifera shells, 10-20% of camellia oleifera leaves, 10-30% of tea bran, 1-10% of eucalyptus branches and leaves, 1-10% of pine byproducts, 0.1-10% of eucalyptus oil and 0.1-10% of fermentation bacteria.

[0010] Preferably, the by-product composition for promoting plant growth and reducing weeds is characterized by comprising the following raw materials in percentage by mass: 30% of camellia oleifera shells, 20% of camellia oil leaves, 30% of tea bran, 5% of eucalyptus branches and leaves, 5% of pine by-products, 5% of eucalyptus oil, and 5% of fermentation bacteria.

[0011] The raw materials of oil tea shell, oil tea leaves, tea bran and pine byproducts are reasonably matched to promote the growth of eucalyptus. The average height of eucalyptus treated by the present invention is 2.34m higher than that treated by commercial compound fertilizer. The incidence of pests and diseases is reduced by more than 18% compared with commercial fertilizers. The present invention has a significant effect on promoting the fruit setting rate of oil tea compared with commercial fertilizers.

[0012] In a preferred case, the proportion of eucalyptus branches and leaves added is 1%-10%, and the proportion of eucalyptus oil added is 0.1%-10%. Experiments show that a reasonable ratio can reduce weeds and plant diseases, with significant effects, and the allelopathic effect on plants is not obvious. Eucalyptus oil has antibacterial effect. When added in small amounts, while improving the hydrophobicity of plant leaf surfaces, eucalyptus oil can also form a synergistic effect with tea bran, thereby forming a composite of organic weed control and insecticide repellent, which is more than 15% higher than single 1,8-cineole weed control.

[0013] Preferably, the fermentation bacteria include one or more of Enterococcus faecalis, Bacillus subtilis, Trametes versicolor, and Trametes rigidus, which significantly reduces the fermentation time of Camellia oleifera shells. The material is heated to 30-60°C, which significantly improves the fermentation effect.

[0014] Preferably, the pine by-products include one or more of pine needles and pine bark.

[0015] Preferably, the eucalyptus oil is a volatile oil extracted from eucalyptus branches and leaves, regardless of species or extraction method.

[0016] The present invention also provides a method for preparing a byproduct composition for promoting plant growth and reducing weeds, comprising the following steps:

[0017] (1) grinding tea oil shells, tea leaves, tea bran, eucalyptus branches and leaves, and pine by-products to a size of 60 mesh or more, and mixing the above raw materials with water in a certain proportion to obtain a mixed solution;

[0018] (2) heating the mixed solution to 30-60° C., leaving it at room temperature, and then adding fermentation bacteria to ferment the mixed solution to obtain a fermented mixed solution;

[0019] (3) filtering the mixed solution after fermentation and adjusting the pH value to 5-8 with a pH regulator;

[0020] (4) Adding the fermentation filtrate to eucalyptus oil and mixing them together, a composition can be obtained.

[0021] Preferably, the ratio of the raw materials to water in step (1) is 1:10-300.

[0022] Preferably, the mixed solution is heated in step (2) by mixing the materials uniformly while heating the materials to 30-60°C.

[0023] Preferably, the fermentation conditions in step (2) are: aerobic fermentation at 25-35° C. for 30-45 days.

[0024] Preferably, the pH adjuster in step (3) comprises one or more of sodium hydroxide and acetic acid.

[0025] In the above preparation method, heating and fermentation with several microorganisms are used to rapidly ferment the agricultural and forestry residues. Preferably, a suitable proportion of eucalyptus oil can be added to the fermentation filtrate obtained in step (4) to further improve the comprehensive performance of weed control and insect repellent.

[0026] Compared with existing agricultural technologies, the present invention has shown significant advantages and technological innovations in many aspects. Specifically, it is reflected in the following aspects:

[0027] (1) The present invention ingeniously makes comprehensive and efficient use of a series of agricultural and forestry residues that were originally regarded as wastes, including tea oil shells, tea leaves, tea bran residues generated during tea oil processing, by-products of pine felling or pruning, and eucalyptus branches and leaves. This not only greatly expands the value-added processing channels of agricultural and forestry resources such as tea oil, pine, and eucalyptus, but also realizes the recycling and sustainable development of resources, marking a significant progress in the field of resource utilization of agricultural and forestry wastes.

[0028] (2) These carefully selected ingredients, such as tea oleifera shells, which are rich in natural organic matter, tea leaves, which contain trace elements that promote plant growth, tea bran, which can effectively improve soil structure due to its unique structure, and pine by-products and eucalyptus branches and leaves, which are rich in bioactive substances, all show extremely high stability. After scientific proportioning, the formula has a very reasonable nutritional structure and can accurately meet the various needs of plants during growth, effectively promote the healthy growth of plants, enhance the resistance of plants to stress, and especially perform well in promoting fruit setting, significantly improving the yield and quality of crops.

[0029] (3) The present invention utilizes byproducts of tea oil processing, such as tea shells, tea leaves, and tea bran, combined with natural resources such as eucalyptus branches and leaves, pine byproducts, and eucalyptus oil, a substance with unique biological activity, and a specific microbial community to prepare a high-performance composition. This innovative combination not only deepens the resource utilization of waste, but also greatly enhances the practical value of ecological agriculture.

[0030] (4) In terms of accelerating the fermentation of camellia oleifera shells, the present invention effectively shortens the traditional fermentation cycle through the synergistic effect of microorganisms, improves the efficiency and quality of converting camellia oleifera shells into organic fertilizers, and thus accelerates the accumulation of soil organic matter. In addition, the composition can significantly promote the growth of eucalyptus and other plants, and by optimizing the soil microenvironment and enhancing the root absorption capacity, the plants are more robust and the leaves are more luxuriant. In terms of promoting fruit setting, its unique biologically active ingredients can regulate the balance of plant hormones, increase the fruiting rate, and bring direct economic benefits to agricultural production.

[0031] (5) The probiotic combination introduced in the present invention, through its unique biological activity, not only greatly shortens the fermentation time of agricultural and forestry residues and improves the processing efficiency, but also can effectively inhibit the growth of weeds, reduce the occurrence of pests and diseases, and create a more favorable ecological environment for the growth of crops. The application of this innovative technology not only reduces the use of chemical pesticides in agricultural production, but also promotes ecological balance, which is an important step in the green transformation of modern agriculture.

[0032] (6) The composition of the present invention can also effectively inhibit the growth of weeds and reduce the dependence on chemical herbicides, which not only reduces agricultural production costs, but also protects the ecological environment and maintains biodiversity. At the same time, the natural ingredients in the composition have an inhibitory effect on a variety of plant diseases and insect pests, reducing the use of pesticides, providing a safer growth environment for crops, and realizing a green prevention and control strategy of controlling diseases and insect pests from the source.

[0033] (7) In terms of soil improvement, the present invention improves the quality of crops by increasing soil microbial activity, improving soil structure, and enhancing soil water and fertilizer retention capacity, such as increasing the sweetness, color, and nutritional value of fruits, making agricultural products more in line with the market demand for high-quality food. In addition, the composition can also significantly improve the stress resistance of crops, including drought resistance, cold resistance, disease resistance, etc., enhance the adaptability of crops to extreme climatic conditions, and ensure the stability of agricultural production.

[0034] (8) From the production process point of view, the entire production process of the present invention abandons the use of harmful chemicals, the whole process is simple, no pungent odor is generated, and it is friendly to operators. At the same time, it also avoids environmental pollution and truly realizes green production. Its cost is relatively low and it is easy to mass produce, which provides the possibility of promotion to the vast rural areas.

[0035] (9) The raw materials of the present invention are all derived from agricultural and forestry residues. These materials are easy to obtain and do not require additional mining or processing, which greatly reduces the production cost. The operation process is simple and clear, and does not require complex equipment or professional skills, making this technology easy to promote and apply in rural areas. More importantly, due to the low cost of raw materials, the price of the final product is more competitive, which is conducive to farmers' acceptance and widespread use.

[0036] (10) Applying the product of the present invention to forest land can not only significantly improve soil structure, increase soil fertility, and enhance the soil's ability to retain water and fertilizer, but also effectively improve the quality of crops, making agricultural products greener and healthier, and meeting the high requirements of modern consumers for food safety and quality. At the same time, this approach also significantly reduces environmental pollution caused by the burning or landfilling of agricultural and forestry wastes, making positive contributions to environmental protection and ecological restoration, and demonstrating the great potential of the present invention in promoting sustainable agricultural development and achieving a win-win situation in economic and ecological benefits.

[0037] (11) The present invention not only significantly improves the level of resource utilization of agricultural waste, but also provides strong technical support for the development of organic ecological agriculture and forestry. It not only conforms to the sustainable development concept currently advocated by the world, but also provides a practical solution for solving agricultural non-point source pollution and promoting agricultural green transformation. It has extremely high feasibility and huge application potential, marking an important step forward in my country's ecological agricultural technology and demonstrating significant scientific and technological progress and innovation capabilities. BRIEF DESCRIPTION OF THE DRAWINGS

[0038] Figure 1 This is a photo of crabgrass before being sprayed with the composition prepared in Example 2 of the present invention;

[0039] Figure 2 This is a photo of the effect of spraying crabgrass with the composition prepared in Example 2 of the present invention 7 days later;

[0040] Figure 3 This is a photo of Phyllanthus urinaria before spraying the composition prepared in Example 2 of the present invention;

[0041] Figure 4 This is a photo of the effect of spraying the composition prepared in Example 2 of the present invention on Phyllanthus urinaria 7 days later;

[0042] Figure 5 This is a photo of a control Camellia oleifera forest flowering period without using the composition prepared in Example 2 of the present invention;

[0043] Figure 6 This is a second photo of the flowering period of a control Camellia oleifera forest without using the composition prepared in Example 2 of the present invention;

[0044] Figure 7 This is a photo of the flowering period of the oil tea forest using the composition prepared in Example 2 of the present invention;

[0045] Figure 8 This is a photo of the oil-tea tree in the fruiting stage in a forest using the composition prepared in Example 2 of the present invention;

[0046] Fig. 9 This is a photo of a tea tree forest at the end of its fruiting period using the composition prepared in Example 2 of the present invention;

[0047] Fig.10 The photos show that the number of bacterial colonies in the embodiments of the present invention is greater than that in the comparative examples. DETAILED DESCRIPTION

[0048] In order to facilitate a better understanding of the present invention, the following examples are used to illustrate the present invention. These examples belong to the protection scope of the present invention, but do not limit the protection scope of the present invention.

[0049] In an embodiment of the present invention, a byproduct composition for promoting plant growth and reducing weeds includes the following raw materials in percentage by mass: 20-30% of camellia oleifera shells, 10-20% of oil tea leaves, 10-30% of tea bran, 1-10% of eucalyptus branches and leaves, 1-10% of pine byproducts, 0.1-10% of eucalyptus oil, and 0.1-10% of fermentation bacteria.

[0050] The fermentation bacteria include one or more of Enterococcus faecalis, Bacillus subtilis, Trametes versicolor, and Trametes rigidus, and contain an effective live cell count of 5×10 -9 -20×10 -9 cfu.

[0051] The pine by-products include one or more of pine needles and pine bark.

[0052] The eucalyptus oil is a volatile oil extracted from eucalyptus branches and leaves, and is not limited to its variety or extraction method.

[0053] The method for preparing the byproduct composition for promoting plant growth and reducing weeds comprises the following steps:

[0054] (1) Crush camellia oleifera shells, tea leaves, tea bran, eucalyptus branches and leaves, and pine by-products to a size of 60 mesh or more, and mix the above raw materials with water in a ratio of 1:10-300 to obtain a mixed solution;

[0055] (2) heating the mixed solution to 30-60° C., leaving it at room temperature, adding fermentation bacteria, and aerobically fermenting it at 25-35° C. for 30-45 days to obtain a fermented mixed solution;

[0056] (3) filtering the mixed solution after fermentation and adjusting the pH value to 5-8 with a pH regulator;

[0057] (4) Adding the fermentation filtrate to eucalyptus oil and mixing them together, a composition can be obtained.

[0058] The mixed solution heating in step (2) is to mix the materials uniformly while heating the materials to 30-60°C.

[0059] The pH adjusting agent in step (3) includes one or more of sodium hydroxide and acetic acid.

[0060] Technical principle of the present invention:

[0061] The present invention is directed to a byproduct composition that promotes plant growth and effectively reduces weeds. Its carefully designed formula contains a combination of natural resources and microorganisms, which is not only environmentally friendly and sustainable, but also has significant effects. The composition contains the following raw materials in mass percentage: 20-30% of camellia oleifera shells, 10-20% of oil tea leaves, 10-30% of tea bran, 1-10% of eucalyptus branches and leaves, 1-10% of pine byproducts, 0.1-10% of eucalyptus oil, and 0.1-10% of fermentation bacteria. Each raw material plays an indispensable role and works together to achieve the dual goals of plant growth and weed suppression:

[0062] (I) Specific role and synergistic mechanism of raw materials

[0063] 1. Specific role of raw materials

[0064] Camellia oleifera shell (20-30%): As the outer skin of the camellia oleifera fruit, the camellia oleifera shell is rich in lignin, cellulose and various trace elements. It can provide organic matter to the soil and improve the soil structure. At the same time, the nutrients released during its decomposition process are helpful for the development of plant roots.

[0065] Camellia oleifera (10-20%): Camellia oleifera is rich in amino acids, vitamins and alkaloids. These ingredients are slowly released in the soil, which can effectively promote plant nutrient absorption, enhance plant resistance and reduce the occurrence of diseases and pests.

[0066] Tea bran (10-30%): Tea bran is a by-product of camellia oil extraction. It contains a high amount of saponin and has a good natural herbicidal effect, which can inhibit the growth of weeds. At the same time, its rich organic matter and trace elements can promote soil microbial activity and improve soil fertility.

[0067] Eucalyptus branches and leaves (1-10%): Eucalyptus branches and leaves are rich in essential oils, which have antibacterial and insecticidal properties, and can further protect plants from diseases and pests. Their decomposition products can also regulate soil pH, creating a more suitable environment for plant growth.

[0068] Pine by-products (1-10%): including pine needles and pine bark, which are rich in rosinic acid, phenolic compounds and trace elements, which can effectively promote the formation of soil aggregate structure and improve the soil's ability to retain water and fertilizer. At the same time, the special substances in pine needles are effective in inhibiting the growth of certain weeds.

[0069] Eucalyptus oil (0.1-10%): Volatile oil extracted from eucalyptus branches and leaves, with a strong aromatic smell, can effectively inhibit the germination of weed seeds and reduce weed competition. At the same time, its natural antiseptic properties can also protect plant roots from diseases.

[0070] Fermentation bacteria (0.1-10%): including Enterococcus faecalis, Bacillus subtilis, Trametes versicolor, Trametes rigidus, etc. These microorganisms can effectively decompose the difficult-to-degrade substances in the raw materials under suitable conditions (such as temperature 25-35°C, aerobic fermentation for 30-45 days), release nutrients that are easily absorbed by plants, and the metabolites produced can further enhance the diversity of soil microorganisms, promote plant growth, and inhibit weeds.

[0071] 2. Synergy between raw materials

[0072] These raw materials achieve multiple synergistic effects through scientific proportioning and fermentation process. Camellia oleifera shells, tea leaves, tea bran, eucalyptus branches and leaves, and pine byproducts provide rich organic matter and trace elements, laying a solid foundation for plant growth; while the addition of eucalyptus oil strengthens the weed suppression effect and reduces nutrient competition. The introduction of fermentation bacteria not only accelerates the decomposition of organic matter, but also produces plant growth-promoting substances and biological pesticides through its metabolic activities, forming a natural plant protection barrier.

[0073] (II) Importance of controlling raw material dosage and optimizing process parameters

[0074] Precisely controlling the amount of each raw material is the key to ensuring the effectiveness of the composition. For example, using too high a ratio of tea leaves, eucalyptus branches and leaves, and tea bran for fermentation may cause the soil pH to drop, affecting the plant's absorption of nutrients; while an appropriate amount of eucalyptus oil can maximize its weed control effect without harming the plants. In addition, the temperature, time and oxygen conditions during the fermentation process must be strictly controlled to optimize microbial activity and ensure the efficiency and stability of the fermentation products. Through meticulous optimization of process parameters, the composition not only achieved the expected growth promotion and weed suppression effects, but also unexpectedly improved the health of the soil ecosystem, reduced the use of fertilizers and pesticides, and demonstrated excellent environmental benefits and agricultural sustainability.

[0075] In order to make the disclosure of the present invention more complete, it is described below through more specific embodiments.

[0076] Example 1

[0077] A byproduct composition for promoting plant growth and reducing weeds comprises the following raw materials in percentage by mass: 30% of camellia oleifera shells, 20% of camellia oleifera leaves, 10% of tea bran, 10% of eucalyptus branches and leaves, 10% of pine byproducts, 10% of eucalyptus oil and 10% of fermentation bacteria.

[0078] The fermentation bacteria are Bacillus subtilis and Enterococcus faecalis in a ratio of 1:1.

[0079] The pine tree by-product is pine needles.

[0080] The method for preparing the byproduct composition for promoting plant growth and reducing weeds comprises the following steps:

[0081] (1) Crush oil tea shells, oil tea leaves, tea bran, eucalyptus branches and leaves, and pine by-products into 60 meshes, and mix the raw materials with water in a ratio of 1:10 to obtain a mixed solution;

[0082] (2) heating the mixed solution to 60° C., leaving it at room temperature, adding Bacillus subtilis and Enterococcus faecalis, and aerobically fermenting it at 25° C. for 45 days;

[0083] (3) filtering the mixed solution after fermentation and adjusting the pH value to 5 with a pH regulator;

[0084] (4) Add the fermentation filtrate to eucalyptus oil and mix them to obtain a composition.

[0085] In step (2), the mixed solution is heated by mixing the materials while heating the materials to 60°C.

[0086] The pH regulator in step (3) is acetic acid.

[0087] Example 2

[0088] A camellia oil byproduct composition for promoting plant growth comprises the following raw materials in percentage by mass: 30% camellia oil shell, 20% camellia oil leaves, 30% tea bran, 5% eucalyptus branches and leaves, 5% pine byproducts, 5% eucalyptus oil and 5% fermentation bacteria.

[0089] The fermentation bacteria are Bacillus subtilis and Trametes versicolor in a ratio of 1:1.

[0090] The pine by-product is pine bark.

[0091] The method for preparing the byproduct composition for promoting plant growth and reducing weeds comprises the following steps:

[0092] (1) The preparation method is as follows: crushing tea husks, tea leaves, tea bran, eucalyptus branches and leaves, and pine byproduct branches and leaves into 60 meshes, and mixing the raw materials with water in a ratio of 1:100 to obtain a mixed solution;

[0093] (2) heating the mixed solution to 30° C., leaving it at room temperature, adding Bacillus subtilis and Trametes versicolor, and aerobically fermenting it at 30° C. for 35 days;

[0094] (3) filtering the mixed solution after fermentation and adjusting the pH value to 8 with a pH regulator;

[0095] (4) Add the fermentation filtrate to eucalyptus oil and mix them to obtain a composition.

[0096] In step (2), the mixed solution is heated by mixing the materials while heating the materials to 30°C.

[0097] The pH regulator in step (3) is sodium hydroxide.

[0098] Example 3

[0099] A tea oil byproduct composition for promoting plant growth and reducing weeds comprises the following raw materials in percentage by mass: 25% tea oil shell, 20% tea leaves, 30% tea bran, 10% eucalyptus branches and leaves, 9% pine byproducts, 5% eucalyptus oil and 1% fermentation bacteria.

[0100] The fermentation bacteria is Trametes rigidus.

[0101] The pine tree by-product is pine needles.

[0102] The method for preparing the byproduct composition for promoting plant growth and reducing weeds comprises the following steps:

[0103] (1) The preparation method thereof is as follows: crushing tea husks, tea leaves, tea bran, eucalyptus branches and leaves and pine by-products into 60 meshes, and mixing the raw materials with water in a ratio of 1:300 to obtain a mixed solution;

[0104] (2) heating the mixed solution to 45°C, allowing it to stand at room temperature, adding Trametes rigidus, and aerobically fermenting it at 35°C for 30 days;

[0105] (3) filtering the mixed solution after fermentation and adjusting the pH value to 6 with a pH regulator;

[0106] (4) Adding the fermentation filtrate to eucalyptus oil, a composition can be obtained.

[0107] The mixed solution heating in step (2) is to mix the materials uniformly while heating the materials to 45°C.

[0108] The pH regulator in step (3) is sodium hydroxide.

[0109] Example 4

[0110] A byproduct composition for promoting plant growth and reducing weeds comprises the following raw materials in percentage by mass: 30% of camellia oleifera shells, 15% of camellia oleifera leaves, 20% of tea bran, 10% of eucalyptus branches and leaves, 10% of pine byproducts, 10% of eucalyptus oil, and 5% of fermented bacteria.

[0111] The fermentation bacteria are composed of Trametes versicolor, Bacillus subtilis and Trametes rigidus in a ratio of 1:1:1.

[0112] The pine tree by-product is pine needles.

[0113] The method for preparing the byproduct composition for promoting plant growth and reducing weeds comprises the following steps:

[0114] (1) grinding tea oil shells, tea leaves, tea bran, eucalyptus branches and leaves, and pine by-products into 60 meshes, and mixing the raw materials with water in a ratio of 1:200 to obtain a mixed solution;

[0115] (2) heating the mixed solution to 50°C, leaving it at room temperature, adding Trametes versicolor, Bacillus subtilis, and Trametes rigidus, and fermenting it aerobically at 35°C for 3 days;

[0116] (3) filtering the mixed solution after fermentation and adjusting the pH value to 7 with a pH regulator;

[0117] (4) Add the fermentation filtrate to eucalyptus oil and mix them to obtain a composition.

[0118] In step (2), the mixed solution is heated by mixing the materials while heating the materials to 50°C.

[0119] The pH adjusting agent in step (3) is sodium hydroxide.

[0120] Comparative Example 1

[0121] It is basically the same as Example 2, except that the preparation raw materials do not contain camellia oleifera shells, tea leaves, tea bran, and pine by-products, and are replaced by peanut bran in the same proportion. The results show that the composition containing camellia oleifera shells, tea bran, and pine by-products can better promote plant growth, reduce pests and diseases, and improve stress resistance.

[0122] Comparative Example 2

[0123] It is basically the same as Example 2, except that the raw material does not contain eucalyptus oil, but is replaced by cinnamon oil or prickly ash oil in the same proportion. The results show that the composition containing eucalyptus oil can better reduce weeds and improve stress resistance.

[0124] Comparative Example 3

[0125] It is basically the same as Example 2, except that the fermentation bacteria are Bacillus licheniformis + Trametes rigidus. The results show that the combinations of Trametes rigidus + Bacillus subtilis, Trametes rigidus + Enterococcus faecalis, and Trametes rigidus + Trametes versicolor can better promote the fermentation of agricultural and forestry residues than the combination of Bacillus licheniformis + Trametes rigidus.

[0126] Comparative Example 4

[0127] It is basically the same as Example 2, except that the temperature of the fermentation raw material mixture is 10°C, 20°C and 70°C. The results show that the material heating temperature at 30-60°C can better promote the fermentation of residues such as camellia oleifera shells.

[0128] Application Experimental Example 1: Effect of the Composition Invention on Promoting Eucalyptus Growth 1.1 Overview of the Experimental Site

[0129] The test site was selected in Zhailu Village, Xiyan Town, Shanglin County, Nanning City, Guangxi Province. The geographical location is 108°42′76″ east longitude and 23°58′19″ north latitude. It belongs to the southern subtropical monsoon climate, with mild light and climate, distinct four seasons, sufficient rainfall, long frost-free period, and significant monsoon climate. The annual average temperature is 21.1℃, the rainfall is abundant and unevenly distributed, and the annual average rainfall is 1714.8 mm. The terrain of the experimental site is mountainous with steep slopes. The soil is mainly acidic loam with average texture. The content of organic matter, available phosphorus, available potassium, and total nitrogen is average. The fertility of the site soil is at a medium to lower level.

[0130] 1.2 Test seedlings

[0131] The afforestation seedlings used in this experiment were Eucalyptus grandis Guanglin No. 9, provided by the Guangxi Zhuang Autonomous Region Forestry Science Research Institute. The eucalyptus seedlings in the test plot were Guanglin No. 9 tissue culture seedlings cultivated by the Guangxi Forestry Science Research Institute, and their size and growth conditions were basically the same (the average seedling height was 38.6 cm and the average ground diameter was 3.94 mm).

[0132] 1.3 Fertilizer application

[0133] No. 1: Commercially available compound fertilizer, compound fertilizer provided by Guangxi Supply and Marketing Modern Agricultural Service Co., Ltd. (total nutrients ≥ 35%, N-P2O5-K2O is 18-5-12)

[0134] No. 2: The byproduct composition for promoting plant growth and reducing weeds prepared in Example 2 of the present invention.

[0135] Control fertilizer: the composition prepared in Comparative Example 1.

[0136] 1.4 Test methods

[0137] 1.4.1 Experimental design

[0138] Compare No. 1, No. 2 and the control fertilizer. The experiment adopted a single-factor random block design test method and set up three groups of repetitions. 30 eucalyptus trees were planted in each experimental plot with a row spacing of 2×3m. An isolation belt was set up in each plot. The experiment was planted in early June 2020, and then topdressing was carried out every year until mid-May 2022, with a fertilizer application rate of 500g / plant. The eucalyptus trees were observed in mid-December 2022, and the tree height of the eucalyptus trees was measured with a measuring rod and the diameter at breast height of the eucalyptus trees was measured with a circumference ruler. The volume of a single tree was calculated using the formula specified by the Guangxi Zhuang Autonomous Region Forestry Survey and Design Institute. [1] .

[0139] Literature: [1] VAN SOEST PJ, ROBERTSON JB, LEWIS B A. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition [J]. Journal of Dairy Science, 1991, 74(10): 3583-3597.

[0140] 1.4.2 Data Processing

[0141] The data obtained were processed using Office Excel 2007. Combining the three test plots of eucalyptus diameter at breast height, wood volume, tree height, average growth such as average height and average diameter at breast height were analyzed.

[0142] Table 1 shows that under different ways of adding fungi, the average tree height, average breast diameter, average timber volume, crown width, preservation rate, and incidence of diseases and insect pests of eucalyptus have significant differences.

[0143] It can be found from the data in Table 1 that under different methods, the average tree height, average timber volume, average breast diameter, crown width, average timber volume, preservation rate, and incidence of pests and diseases of eucalyptus are significantly different from those of the control. Among them, under measure No. 2, the growth traits of eucalyptus are better, and the average tree height can reach 13.66m. At the same time, the incidence of pests and diseases is the composition of the present invention, which is 10.88%, indicating that the economic traits and growth traits of the eucalyptus treated in this way are the best.

[0144] Table 1 Effects of different fertilization treatments on eucalyptus growth

[0145]

[0146]

[0147] Application Experimental Example 2: Effect of the present invention on reducing weeds in oil-tea plantations

[0148] 1 Materials and methods

[0149] 1.1 Overview of the test site

[0150] The experimental site is located in the oil-tea high-yield demonstration base in Nalu Village, Youjiang District, Baise City, Guangxi. The experimental mountain has a steep terrain, and the content of organic matter, quick-acting phosphorus, quick-acting potassium, and total nitrogen is average. The fertility of the site soil is at a lower-medium level, and the soil is weakly acidic with a pH of 6.9.

[0151] 1.2 Experimental design

[0152] The experiment was conducted in four treatments according to the type and dosage of the applied agent, namely: Treatment A (Comparative Example 2), cinnamon oil replaced eucalyptus oil, and other preparation methods and the same composition of the present invention were 15 L / hm 2 ; Treatment B (Comparative Example 2), prickly ash oil replaced eucalyptus oil, other preparation methods and the same composition of the present invention 15L / hm 2 ; Treatment C, the composition prepared in Example 2 of the present invention 15L / hm 2 ; Treatment D, 1,8 cineole 15L / hm 2 ; Treatment F, clean water control (CK).

[0153] There were no replicates for each treatment. Each treatment area had 30 tea trees in one row, for a total of 3 rows, and single row and single irrigation. The tea tree forest was transplanted in late December 2018, and root irrigation was used once from April 2021. Observations were made on the 3rd, 7th, and 14th days, and the watering rate for each treatment was 300L / hm 2 , CK sprayed with an equal amount of water. The weed control effect was investigated in mid-April 2021, and the fruit setting rate was observed at the end of November. Observe the response of weeds to the composition: 7 days after application, investigate the number of weeds and calculate the control effect; 14 days after application, investigate the number of weeds and fresh weight, and calculate the control effect and fresh weight control effect respectively. Calculation formula: Control effect (fresh weight) = {[number of weeds in the water control area (fresh weight) - number of weeds in the preparation treatment area (fresh weight)] ÷ number of weeds in the water control area (fresh weight)} × 100%. The experimental data were statistically analyzed using DPS software.

[0154] 2 Results and analysis

[0155] 2.1 Security

[0156] According to observations during the test, the growth and development of tea oil plants in treatments A, B, and C were normal, with no signs of phytotoxicity. Three days after application, the weed leaves turned green and yellow, and seven days after application, the base of the weed stems rotted and turned brown, and the entire weed plant fell over (see Figure 1-4 ); 14 days after application, the whole weed plant died.

[0157] 2.2 Weed Suppression Effect

[0158] The results in Table 2 show that compared with treatment A and treatment B, the present invention has excellent weed control effect on weeds around oil tea, and is significantly more effective than treatment A and treatment B against common leaf urinary grass, crabgrass and goosegrass in the area. The 14d control effect of the present invention on crabgrass is 47% and 35.8% higher than that of treatment A and treatment B, respectively, the control effect on leaf urinary grass is 31.2% and 25.9% higher than that of treatment A and treatment B, respectively, and the control effect on goosegrass is 40.5% and 37.6% higher than that of treatment A and treatment B, respectively.

[0159] Table 2 Effect of different treatments on weed control

[0160]

[0161] The results in Table 3 show that compared with treatment A and treatment B, the present invention has excellent control effect on weeds around oil tea, and the fresh weight control effect can reach more than 50% 14 days after application. Treatment C has significant difference in fresh weight control effect on each weed compared with treatment A and treatment B, and the difference in fresh weight control effect of treatment C with treatment A and treatment B reaches a significant level.

[0162] Table 3 Fresh weight control effect of different treatments on weeds (14 days after application)

[0163] deal with Crabgrass Phyllanthus urinaria Goosegrass A 19.3％a 18.7％a 15.7％a B 25.5％a 26.7％a 23.5％a C 57.8％b 54.1％b 62.6％b D 44.2％b 41.8％b 47.9％b

[0164] Note: Different lowercase letters after the data in the same column in the table indicate significant differences among treatments.

[0165] Application Experimental Example 3: The Effect of the Present Invention on Promoting the Fruit Setting Rate and Fruit Retention Rate of Camellia oleifera 1 Materials and Methods

[0166] 1.1 Overview of the test site

[0167] The test site is located in the oil-tea high-yield demonstration base of Nalu Village, Youjiang District, Baise City, Guangxi. The test mountain is steep, with average content of organic matter, available phosphorus, available potassium and total nitrogen. The fertility of the site soil is below average, the soil is slightly acidic, and the pH is 6.9. It has a subtropical humid monsoon climate, with abundant light and heat, rain and heat in the same season, long summer and short winter, an average annual temperature of 22℃, no severe cold in winter, the average temperature in the coldest month of January is 13℃, and the average minimum temperature is 10℃.

[0168] 1.2 Test materials

[0169] 1.2.1 Test plants The test oil tea variety is Cenruan Oil Tea No. 2, which is widely planted locally. In the same tea forest of the oil tea demonstration base, oil tea trees with a tree age of 3 years, normal growth and development, and roughly the same growth potential, crown width, and tree shape were selected as test objects. The row spacing specification of oil tea planting was 3m×4m, and the management of the oil tea forest during the test was consistent with the local conventional level.

[0170] 1.2.2 Plant nutrients for testing

[0171] One kind of nutrient fertilizer was selected for the experiment. The nutrient fertilizer was humic acid water-soluble fertilizer produced by Shandong Zhengyuan Fertilizer Co., Ltd. The concentration was 1000 times diluted.

[0172] 1.3.1 Experimental Design A single-factor randomized block experiment was conducted with the above-mentioned nutrient fertilizer and the present invention as treatment objects, clear water as control (CK), positive source fertilizer as treatment A, the present invention (Example 2) as treatment B, and clear water control as treatment C. According to the experimental design, 30 plants were selected in each plot, and each treatment was repeated 3 times. The oil tea branches with the same growth potential and length in the middle of the plant were selected in the north-south direction as standard branches, and 2 standard branches were selected for each oil tea plant for determination of relevant indicators.

[0173] 1.3.2 Methods Root irrigation was used. Before root irrigation, the test plants and standard branches were numbered, marked, and labeled. In view of the characteristics of fruit drop during the young and middle fruit stages of Camellia oleifera fruit growth, root irrigation was carried out in early November 2020 (flowering period, see Figure 5-7 ), mid-April 2021 (young fruit stage) and late July 2021 (middle fruit stage, see Figure 8 Choose a sunny or cloudy day with no wind or light wind, and sprinkle 3L per plant between 9:00 and 16:00.

[0174] 1.3.3 Index determination: record the total number of flowering on standard branches before spraying in November 2020, record the number of young fruits in April 2021, record the number of medium-sized fruits in July 2021, record the number of harvested fruits in November 2021, and record the number of fruit at the end of the period. Fig. 9 . Calculate the fruit setting rate, mid-term fruit retention rate and final fruit retention rate.

[0175] Fruit setting rate = average number of young fruits per plant / average number of flowers per plant × 100%

[0176] Mid-term fruit retention rate = mid-term fruit number / young fruit number × 100%

[0177] The final fruit retention rate = the number of fruits collected / the number of young fruits × 100%

[0178] Single fruit seed yield = single fruit seed weight / single fruit weight × 100%

[0179] 1.4 Data Statistics and Analysis

[0180] The experimental data were statistically analyzed using IBM SPSS Statistics 26.0.

[0181] 2 Results and analysis

[0182] 2.1 Fruit setting rate and fruit retention rate of Camellia oleifera with different treatments

[0183] As shown in Table 4, there are certain differences in the fruit setting rate and fruit retention rate of Camellia oleifera among different treatments.

[0184] Table 4 Fruit setting rate and fruit retention rate of Camellia oleifera under different treatments

[0185]

[0186] 2.1.1 Fruit setting rate

[0187] Treatment C was 30.8%, and the treatment of the present invention was the highest, with a fruit setting rate of 45.2%, 15% higher than that of treatment C, with a significant difference (P < 0.05). The fruit setting rate of treatment A was 11.3% lower than that of the present invention, indicating that the composition of the present invention has a significant promoting effect on the fruit setting of oil tea.

[0188] 2.1.2 Medium-term fruit retention rate

[0189] The mid-term fruit retention rate of the present invention is the highest, which is 67.2%; the mid-term fruit retention rates of treatment A and treatment C are relatively low, and the mid-term fruit retention rate of the treatment of the present invention is significantly higher than that of treatment A and treatment C, and there is no significant difference between treatment A and treatment C. This shows that the composition of the present invention has a significant promoting effect on the mid-term fruit retention rate of oil tea.

[0190] 2.1.3 Fruit retention rate at the end of the season

[0191] The final fruit retention rate of the treatment of the present invention was the highest, at 58.8%, which was 7.1% higher than that of treatment A and 19% higher than that of treatment C, with significant differences (P<0.05), indicating that the composition of the present invention has a significant promoting effect on the final fruit retention rate of oil tea.

[0192] Application Experiment 4: Fermentation method rapidly promotes the decomposition of raw materials such as camellia oleifera shells and eucalyptus leaves

[0193] 1 Materials and Instruments

[0194] 1.1 Materials

[0195] Trametes trogii, Bacillus subtilis, Trametes versicolor, Enterococcus faecalis, and Aspergillus flavus were provided by the Institute of Soil and Fertilizer, Guangxi Academy of Forestry. Ammonia, urea, and tartaric acid were all of analytical grade.

[0196] 1.2 Instruments: TDL-5 high-speed centrifuge, 96-well polystyrene ELISA plate, ELISA detector (power wavexs), electric constant temperature incubator (model DC-1006).

[0197] 2 Experimental methods

[0198] 2.1 Culture of Trametes rigidus

[0199] Inoculate Trametes rigidus into PDA agar medium and culture at 30°C for 48 hours. The medium composition is 200g potato, 20g glucose, 1520g agar, 1000ml water, and the pH value is natural.

[0200] 2.2 Bacillus subtilis culture

[0201] The Bacillus preserved in the glycerol tube was inoculated into the nutrient agar medium and cultured at 30°C for 48 hours. The medium composition: 1L distilled water, 20g glucose, 15g peptone, 5g sodium chloride, 0.5g beef extract, 20g agar, pH 7.4.

[0202] 2.3 Trametes versicolor culture

[0203] The preserved lactic acid bacteria were inoculated into a special culture medium for lactic acid bacteria: Potato extract: 200.0 g of peeled potatoes were cut into small pieces, and 1000.0 mL of water was added and boiled for 30 min. The potato pieces were filtered out and the filtrate was filled to 1000.0 mL.

[0204] 2.4 Enterococcus faecalis culture

[0205] Inoculate in special culture medium: 15.0 g tryptone, 5.0 g soytone, 5.0 g NaCl, 15.0 g agar

[0206] Distilled water 1000.0 mL, pH 7.3.

[0207] 2.5 Aflatoxin culture

[0208] Inoculate in a special culture medium: crush some barley malt, add 4 times the weight of water to the malt, stir evenly, saccharify in a 55℃-60℃ incubator for 4 hours, take out and filter to get the wort, measure the volume and concentration (Bé) of the wort, and then divide it into sterilized triangular bottles. Sterilize at 121℃ for 15 minutes and set aside.

[0209] 2.6 Fermentation broth biomass determination

[0210] 50g of each of tea oil shell and eucalyptus leaves were mixed with deionized water at a ratio of 1:30 (m:V), and seed liquids of Trametes rigidus, Bacillus subtilis, Trametes versicolor, Enterococcus faecalis and CKI-CK5 were inoculated (according to 10% inoculation amount) and fermented at 35°C. The fermented liquid was taken at 15 days of fermentation and vibrated for 2 hours, and the supernatant was filtered with filter paper, and then dried at 100°C to constant weight and weighed. The decomposition effect of different strains and different fermentation times on the main components of the present invention was detected.

[0211] 3 Results and analysis

[0212] Table 5 Effects of different strains and material heating temperatures on residue fermentation

[0213]

[0214]

[0215]

[0216] 4 Conclusion and discussion

[0217] As shown in Table 5, the biomass of the bacteria obtained by fermentation with Trametes bisporus + Enterococcus faecalis increased significantly, especially on the 15th day of fermentation when the material heating temperature was 50°C, the biomass reached the maximum value, and the fermentation time was significantly shortened. The biomass of the bacteria obtained by fermenting tea oil shells with yeast and Trametes bisporus + yeast was less than that with Trametes bisporus + Bacillus subtilis, Trametes bisporus + Enterococcus faecalis, and Trametes bisporus + lactic acid bacteria, and the difference was significant (p < 0.01).

[0218] The invention adopts Trametes rigidus, Enterococcus faecalis, lactic acid bacteria and Bacillus subtilis to ferment the oil tea shells, which are agricultural and forestry residues, to increase the biomass, thereby shortening the fermentation time.

[0219] Using the embodiment of the present invention ( Fig.10 The number of bacteria marked in 20) is greater than that in the control group ( Figure 1 19).

[0220] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A byproduct composition for promoting plant growth and reducing weeds, characterized in that: The invention comprises the following raw materials in percentage by mass: 20-30% of camellia oleifera shell, 10-20% of oil tea leaves, 10-30% of tea bran, 1-10% of eucalyptus branches and leaves, 1-10% of pine byproducts, 0.1-10% of eucalyptus oil and 0.1-10% of fermentation bacteria.

2. The by-product composition for promoting plant growth and reducing weeds according to claim 1, characterized in that: The invention comprises the following raw materials in percentage by mass: 30% of oil tea shell, 20% of oil tea leaves, 30% of tea bran, 5% of eucalyptus branches and leaves, 5% of pine by-products, 5% of eucalyptus oil and 5% of fermentation bacteria.

3. The byproduct composition for promoting plant growth and reducing weeds according to claim 1 or 2, characterized in that: The fermentation bacteria include one or more of Enterococcus faecalis, Bacillus subtilis, Trametes versicolor, and Trametes rigidus, and contain an effective live cell count of 5×10 -9 -20×10 -9 cfu.

4. The byproduct composition for promoting plant growth and reducing weeds according to claim 1 or 2, characterized in that: The pine by-products include one or more of pine needles and pine bark.

5. The by-product composition for promoting plant growth and reducing weeds according to claim 1 or 2, characterized in that: The eucalyptus oil is a volatile oil extracted from eucalyptus branches and leaves, and is not limited to its variety or extraction method.

6. A method for preparing the by-product composition for promoting plant growth and reducing weeds according to any one of claims 1 to 5, characterized in that: The following steps are involved: (1) grinding tea oil shells, tea leaves, tea bran, eucalyptus branches and leaves, and pine by-products to a size of 60 mesh or more, and mixing the above raw materials with water in a certain proportion to obtain a mixed solution; (2) heating the mixed solution to 30-60° C., leaving it at room temperature, and then adding fermentation bacteria to ferment the mixed solution to obtain a fermented mixed solution; (3) filtering the mixed solution after fermentation and adjusting the pH value to 5-8 with a pH regulator; (4) Adding the fermentation filtrate to eucalyptus oil and mixing them together, a composition can be obtained.

7. The method for preparing the by-product composition for promoting plant growth and reducing weeds according to claim 6, characterized in that: The ratio of the raw materials to water in step (1) is 1:10-300.

8. The method for preparing the by-product composition for promoting plant growth and reducing weeds according to claim 6, characterized in that: The mixed solution heating in step (2) is to mix the materials uniformly while heating the materials to 30-60°C.

9. The method for preparing the by-product composition for promoting plant growth and reducing weeds according to claim 6, characterized in that: The fermentation conditions in step (2) are: aerobic fermentation at 25-35° C. for 30-45 days.

10. The method for preparing the by-product composition for promoting plant growth and reducing weeds according to claim 6, characterized in that: The pH adjusting agent in step (3) includes one or more of sodium hydroxide and acetic acid.

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