Preparation method of damp-clearing poria cocos composite nutritional blend oil

Abstract

The invention relates to the technical field of compound nutritional blend oil, in particular to a preparation method of damp-clearing poria cocos compound nutritional blend oil. S1.1, weighing 25 to 30 parts by weight of the modified poria cocos compound, 20 to 25 parts by weight of rhizoma atractylodis macrocephalae, 10 to 15 parts by weight of rhizoma alismatis, 100 to 105 parts by weight of walnut oil, 150 to 155 parts by weight of soybean oil, 200 to 205 parts by weight of linseed oil, 10 to 20 parts by weight of a sodium hydroxide solution and 30 to 40 parts by weight of an ethyl acetate solvent; S1.1, preparing a rhizoma atractylodis macrocephalae and rhizoma alismatis dampness-clearing extract; and S1.2, mixing the rhizoma atractylodis macrocephalae and rhizoma alismatis dampness-clearing extract with the modified poria cocos compound, and adding the mixture into the blend oil to obtain the dampness-clearing poria cocos compound nutritional blend oil. Myristic acid and oleic acid are adopted for esterification modification of pachymaran, hydrophobic groups are introduced, dispersibility and solubility are improved, chitosan is used for crosslinking modification, and stability of pachymaran is enhanced.

Description

Technical Field

[0001] The invention relates to the technical field of composite nutritional blended oil, in particular to a preparation method of dampness-removing Poria composite nutritional blended oil. Background Art

[0002] Poria cocos, as a traditional Chinese medicinal material with a long history, is widely praised for its unique medicinal value. It is mild in nature, sweet and light in taste, and enters the heart, lung, spleen, and kidney meridians. It has the effects of promoting diuresis and eliminating dampness, strengthening the spleen and calming the mind. It is often used to treat symptoms such as edema, oliguria, phlegm and fluid, dizziness and palpitations, spleen deficiency and poor appetite, loose stools and diarrhea, as well as restlessness, palpitations and insomnia. It has a wide range of applications and is deeply loved by doctors and the public.

[0003] Although Poria cocos is highly respected in the field of traditional Chinese medicine for its excellent effects of promoting diuresis and dispelling dampness, strengthening the spleen and calming the mind, the traditional application of Poria cocos, such as directly using it as medicine or making tea or decoction, often has low absorption efficiency, a monotonous taste, and is difficult to integrate with modern people's lifestyles, resulting in low solubility, stability and utilization of its active ingredients. In view of this, we proposed a preparation method for a dehumidifying Poria cocos composite nutritious blending oil. Summary of the invention

[0004] The object of the present invention is to provide a method for preparing a dehumidifying Poria cocos composite nutrient blended oil to solve the problems raised in the above-mentioned background technology.

[0005] To achieve the above object, the present invention provides a method for preparing a dehumidifying Poria cocos composite nutritional blending oil, S1.1, weighing the following raw materials in parts by weight: 25-30 parts by weight of a modified Poria cocos complex, 20-25 parts by weight of Atractylodes macrocephala, 10-15 parts by weight of Rhizoma Alismatis, 100-105 parts by weight of walnut oil, 150-155 parts by weight of soybean oil, 200-205 parts by weight of linseed oil, 10-20 parts by weight of a sodium hydroxide solution, and 30-40 parts by weight of an ethyl acetate solvent; The modified Poria cocos complex is prepared by esterification of Poria cocos polysaccharide extract with oleic acid and myristic acid in a mass ratio of 1:1 to 3:3 to 5, and then cross-linking with chitosan. S1.2. Grind Atractylodes macrocephala and Rhizoma Alismatis into fine powder, add sodium hydroxide solution with a concentration of 0.05-0.1% to moisten for 3-4 hours, then add ethyl acetate solvent to the mixed powder, stir and mix evenly, place in an ultrasonic cleaner, extract components under the action of ultrasound, after the extraction is completed, filter the extract with a Buchner funnel to remove solid residues, then transfer the filtered extract to a rotary evaporator for evaporation, adjust the vacuum pump to a vacuum degree of 100-200 mbar, and evaporate the solvent in a water bath at 50-60°C until the solvent is completely evaporated to obtain Atractylodes macrocephala and Rhizoma Alismatis dehumidifying extracts; S1.3. Fully mix the dehumidifying extracts of Atractylodes macrocephala and Alisma orientalis with the modified Poria cocos complex, and fully mix the walnut oil, soybean oil and linseed oil and heat them to 40-50°C. Then divide the mixed extract into 5 equal parts and add them to the mixed oil in sequence. Before adding the next part of the extract each time, ensure that the previous part of the extract has been completely dispersed, and use an electric stirrer to stir continuously at a stirring rate of 500-800r / min and a stirring time of 10-20min to obtain a dehumidifying Poria cocos composite nutritious blending oil.

[0006] Atractylodes is a commonly used Chinese medicinal material, mainly derived from the dried rhizomes of perennial herbs. It has a variety of biologically active ingredients. For example, atractylodes lactone is one of the most famous active ingredients in Atractylodes, and is believed to have a variety of pharmacological effects, including strengthening the spleen, drying dampness, and promoting diuresis. Atractylodes also contains volatile oils, whose main components include atractylodesone and atractylodes alcohol, which are aromatic and help improve the function of the digestive system, thereby helping to remove dampness. Atractylodes lactone, due to its molecular structure, usually has a low solubility in water and a high solubility in organic solvents. Volatile oils are also easily soluble in organic solvents due to their hydrophobicity. Therefore, using ethyl acetate as a solvent for extraction can more effectively extract the dehumidifying components in Atractylodes, thereby improving the extraction efficiency. In addition, due to the hydrophobicity of these components, they can be directly dissolved in the blending oil when added to the blending oil without the need for special modification, which is convenient for production and utilization.

[0007] Alisma is a commonly used Chinese medicine, also known as Water Alisma, Narcissus and Water Fragrance. It is mainly used for diuresis, dehumidification and swelling. It contains a variety of ingredients, among which the ingredients related to the dehumidifying effect mainly include triterpenoids, volatile oils, phytosterols, fatty acids, etc. These ingredients are also hydrophobic, so ethyl acetate is used as solvent for extraction. At the same time, they also have good solubility in blended oil, so they can be directly added to blended oil to improve the dehumidifying functional characteristics of blended oil.

[0008] Walnut oil is a high-quality edible oil extracted from walnut kernels. It is rich in unsaturated fatty acids, vitamins and minerals. The rich unsaturated fatty acids in walnut oil can help regulate the body's cholesterol content, increase high-density lipoprotein cholesterol, lower triglycerides in the blood, soften blood vessels, and have a certain preventive effect on coronary heart disease and kidney disease caused by arteriosclerosis. In addition, squalene, flavonoids and polyphenol compounds in walnut oil can promote blood circulation, activate body functional cells, enhance vasodilation, improve vascular microcirculation, and protect cells from free radical damage. At the same time, the linolenic acid in walnut oil can also effectively supplement the elements needed by the body and provide nutrition for intellectual development and nervous system development. Therefore, walnut oil is a very healthy edible oil, and moderate intake can bring many benefits to the body.

[0009] Soybean oil is a vegetable oil extracted from soybean seeds. Due to its wide range of uses and economy, it is one of the most commonly used cooking oils worldwide. Soybean oil is rich in fatty acids, vitamins and other ingredients. Unsaturated fatty acids help maintain the health of the cardiovascular system, regulate blood lipid levels, and reduce the risk of cardiovascular disease. Vitamin E has strong antioxidant capacity and helps protect cells from free radical damage. Soybean oil also has the effect of anthelmintic and laxative, and can treat intestinal obstruction and constipation. Therefore, it is an important component in the preparation of blended oil.

[0010] Flaxseed oil is a vegetable oil extracted from flax seeds. It is famous for its high content of α-linolenic acid and has many benefits to the human body. The content of α-linolenic acid in flaxseed oil accounts for about 39% to 60% of the total fatty acids. It is an essential fatty acid for the human body and can be converted into eicosapentaenoic acid and docosahexaenoic acid in the human body. It has anti-tumor, anti-thrombotic, lipid-lowering and brain cell nourishing effects. It helps to regulate blood lipid levels, lower blood cholesterol, maintain heart health, reduce the risk of cardiovascular disease, and relieve inflammation-related diseases. Therefore, incorporating it into your daily diet in moderation can bring many benefits to your health.

[0011] Preferably, in S1.2, the Atractylodes macrocephala and Alismatis rhizome powders are mixed with ethyl acetate in a mass ratio of 1:10 to 12.

[0012] Preferably, in S1.2, the ultrasonic frequency is set to 35-40 kHz and the extraction time is 30-50 min.

[0013] Preferably, the steps for preparing the modified Poria cocos complex are as follows: S2.1, weigh 100 parts by weight of Poria cocos and grind it into fine powder with a high-speed grinder, add water for extraction, extract at 80°C-100°C for 1-2h, filter the extract, remove solid residue, transfer the filtrate to a rotary evaporator for concentration, evaporate the water in the filtrate at 50-60°C, and stir the filtrate continuously until the solvent is completely evaporated to obtain Poria cocos polysaccharide extract; S2.2, heat myristic acid and oleic acid to 60-65 ° C to make them molten, then add the Poria polysaccharide extract, continue stirring, and add sulfuric acid as a catalyst, continue stirring to make it evenly dispersed, place the reaction mixture in a constant temperature water bath, set the temperature to 60-80 ° C, and continue the reaction for 6-8 hours. After the reaction is completed, turn off the heating source, let the mixture cool naturally to room temperature, add sodium hydroxide solution dropwise to the reaction mixture to adjust the pH, then add the neutralized mixture to a separatory funnel, add anhydrous ethanol extraction solvent, shake and let stand for 1-2 hours to separate, collect the upper organic phase, repeat the extraction process 2-3 times until the extraction is complete, and obtain an esterified Poria polysaccharide solution; S2.3. Weigh 5 parts by weight of chitosan and dissolve it in 100 mL of dilute acetic acid solution, and add the chitosan solution drop by drop into the esterified Poria polysaccharide solution while stirring continuously. Adjust the pH to 5-6 with dilute acetic acid solution, and place the reaction system in a constant temperature water bath at 30-40°C for 2-3 hours. Transfer the reaction mixture to a centrifuge tube, centrifuge it at 3000-5000 rpm / min for 10-15 minutes, collect the precipitate at the bottom, wash the precipitate with deionized water, and dry it in a vacuum drying oven at 40-50°C to obtain a modified Poria complex.

[0014] Poria is a traditional Chinese medicine with the effects of removing dampness from phlegm, promoting diuresis and reducing swelling, strengthening the spleen and replenishing qi, and calming the mind. The main dehumidifying effect in Poria is Poria polysaccharide, which is mainly composed of β-(1→3) linked glucose units. It is easily soluble in water, but insoluble in organic solvents such as ethanol, acetone and ether. In addition to its dehumidifying effect, Poria polysaccharide can also enhance the cytotoxic effect of macrophages and T lymphocytes, thereby activating the body's immune surveillance system to fight tumors. It also has the ability to scavenge free radicals, which helps with anti-oxidation and anti-aging. Therefore, it is a very important component. Extracting it and dissolving it in blending oil can effectively improve the nutritional content and dehumidifying function of blending oil, bringing many benefits to the body.

[0015] Myristic acid is a saturated straight-chain fatty acid containing 14 carbon atoms. It is widely present in nature, especially in vegetable oils and animal fats. Myristic acid is used in the food industry, cosmetics and personal care products. In food, it is used as a flavoring agent and thickener, and in cosmetics and personal care products, it is used as an emulsifier or stabilizer. Myristic acid can undergo esterification reaction with the hydroxyl group (-OH) on the surface of Poria cocos polysaccharide to introduce hydrophobic fatty acid chains, thereby increasing the solubility of Poria cocos polysaccharide in the oil phase.

[0016] Oleic acid is a monounsaturated fatty acid that is widely found in animal and vegetable oils, especially in olive oil, rapeseed oil and peanut oil. Its structural characteristic is that it contains a double bond located at the ninth position of the carbon chain. Oleic acid can undergo esterification reaction with the hydroxyl groups on the surface of Poria cocos polysaccharide to introduce hydrophobic fatty acid chains, thereby increasing the solubility of Poria cocos polysaccharide in the oil phase.

[0017] Chitosan is a natural polysaccharide obtained by deacetylation of chitin. It has good biocompatibility, biodegradability and antibacterial properties. Therefore, it is widely used in many fields such as food, medicine, cosmetics and environmental engineering. Chitosan can form a chitosan complex with esterified Poria cocos polysaccharide, introducing hydrophobic groups into chitosan, improving its solubility in oil and enhancing the stability of the Poria cocos polysaccharide structure.

[0018] Through the chemical esterification reaction between Poria polysaccharide and myristic acid and oleic acid in the presence of a catalyst, the carboxyl group (-COOH) of myristic acid or oleic acid reacts with the hydroxyl group (-OH) in Poria polysaccharide to form an ester bond (-COO-), while releasing water molecules (H2O). The introduction of hydrophobic groups increases the hydrophobicity of Poria polysaccharide. In addition, since chitosan molecules contain a large number of amino groups (-NH2) and hydroxyl groups (-OH), chitosan can form hydrogen bonds with the unreacted hydroxyl groups on Poria polysaccharide for cross-linking to form a modified Poria complex. In addition, since the ester group is introduced, the substance is more evenly dispersed in oils and fats.

[0019] Preferably, in S2.1, the high-speed pulverizer is set to have a rotation speed of 10000-20000 r / min, and a pulverizing time of 1-5 min.

[0020] Preferably, in S2.1, the Poria cocos powder and water are mixed in a mass ratio of 1:8 to 10.

[0021] Preferably, in S2.2, the mass fraction of the catalyst sulfuric acid is 96-98%, and the added amount is 1%-5% of the total mass of the Poria polysaccharide extract and myristic acid.

[0022] Preferably, in S2.2, the mass fraction of the sodium hydroxide solution is 5%-10%, and the pH value is adjusted to 6-7.

[0023] Preferably, in S2.3, the mass fraction of the dilute acetic acid solution is 1-5%.

[0024] Preferably, in S2.3, the mass ratio of the esterified Pachymaran solution to the chitosan solution is 1:0.5-1.5.

[0025] Compared with the prior art, the present invention has the following beneficial effects: In the preparation method of the dehumidifying Poria cocos composite nutritional blended oil, myristic acid and oleic acid are used to firstly esterify Poria cocos polysaccharide, and then chitosan is used to cross-link to prepare a modified Poria cocos complex, and a hydrophobic group is introduced through the esterification reaction, so that the modified Poria cocos polysaccharide can be better dispersed and dissolved in the blended oil, and the addition of chitosan further enhances the stability of Poria cocos polysaccharide in the blended oil. This is because chitosan itself has certain antibacterial and antioxidant properties, and thus can effectively protect the dehumidifying components in Poria cocos from oxidation, thereby allowing the modified Poria cocos complex to remain stable for a long time in the blended oil, thereby extending the shelf life of the blended oil. DETAILED DESCRIPTION

[0026] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0027] A preparation method of a dampness-removing Poria cocos composite nutrient blended oil of the present invention: S1.1, weigh the following raw materials in parts by weight: 25-30 parts by weight of modified Poria cocos complex, 20-25 parts by weight of Atractylodes macrocephala, 10-15 parts by weight of Rhizoma Alismatis, 100-105 parts by weight of walnut oil, 150-155 parts by weight of soybean oil, 200-205 parts by weight of linseed oil, 10-20 parts by weight of sodium hydroxide solution and 30-40 parts by weight of ethyl acetate solvent; The modified Poria cocos complex is prepared by esterification of Poria cocos polysaccharide extract with oleic acid and myristic acid in a mass ratio of 1:1 to 3:3 to 5, and then cross-linking with chitosan. S1.2. Grind Atractylodes macrocephala and Rhizoma Alismatis into fine powder, add sodium hydroxide solution with a concentration of 0.05-0.1% to moisten for 3-4 hours, then add ethyl acetate solvent to the mixed powder, stir and mix evenly, place in an ultrasonic cleaner, extract components under the action of ultrasound, after the extraction is completed, filter the extract with a Buchner funnel to remove solid residues, then transfer the filtered extract to a rotary evaporator for evaporation, adjust the vacuum pump to a vacuum degree of 100-200 mbar, and evaporate the solvent in a water bath at 50-60°C until the solvent is completely evaporated to obtain Atractylodes macrocephala and Rhizoma Alismatis dehumidifying extracts; S1.3. Fully mix the dehumidifying extracts of Atractylodes macrocephala and Alisma orientalis with the modified Poria cocos complex, and fully mix the walnut oil, soybean oil and linseed oil and heat them to 40-50°C. Then divide the mixed extract into 5 equal parts and add them to the mixed oil in sequence. Before adding the next part of the extract each time, ensure that the previous part of the extract has been completely dispersed, and use an electric stirrer to stir continuously at a stirring rate of 500-800r / min and a stirring time of 10-20min to obtain a dehumidifying Poria cocos composite nutritious blending oil.

[0028] Example 1 A method for preparing a dampness-removing tuckahoe composite nutrient blended oil comprises the following steps: S3.1. Weigh the following raw materials in parts by weight: 30 parts by weight of modified Poria complex, 20 parts by weight of Atractylodes macrocephala, 10 parts by weight of Alisma orientalis, 100 parts by weight of walnut oil, 150 parts by weight of soybean oil and 200 parts by weight of linseed oil, wherein the modified Poria complex is prepared by esterification reaction of Poria polysaccharide extract with oleic acid and myristic acid in a mass ratio of 1:1:3, and then cross-linking and modification with chitosan, the mass ratio of the esterified Poria polysaccharide solution to the chitosan solution is 1:0.5, the amount of catalyst sulfuric acid added is 1% of the total mass of Poria polysaccharide extract and myristic acid, and Atractylodes macrocephala and Alisma orientalis powder are mixed with ethyl acetate in a mass ratio of 1:10.

[0029] S3.2. Preparation of modified Poria cocos complex: weigh 100 parts by weight of Poria cocos and grind it with a high-speed grinder at 10,000 r / min for 5 minutes to form powder. Add water for extraction at a mass ratio of Poria cocos powder to water of 1:8, extract at 80°C for 2 hours, filter the extract to remove solid residue, transfer the filtrate to a rotary evaporator for concentration, evaporate the water in the filtrate at 50°C, and stir the filtrate continuously until the solvent is completely evaporated to obtain Poria cocos polysaccharide extract; heat myristic acid and oleic acid to 60°C to make them molten, then add the Poria cocos polysaccharide extract, continue stirring, and add 98% by mass sulfuric acid as a catalyst, continue stirring to make it evenly dispersed, place the reaction mixture in a constant temperature water bath, set the temperature to 70°C, and continue the reaction for 6 hours. After the reaction is completed, turn off the heating source, let the mixture cool naturally to room temperature, and return to the reaction mixture. A 5% sodium hydroxide solution was added dropwise to the mixture to adjust the pH to 7, and then the neutralized mixture was added to a separatory funnel, anhydrous ethanol extraction solvent was added, and the mixture was shaken and allowed to stand for 1 hour to separate the layers, and the upper organic phase was collected. The extraction process was repeated 3 times until the extraction was complete to obtain an esterified Poria polysaccharide solution; 5 parts by weight of chitosan was weighed and dissolved in 100 mL of a 1% dilute acetic acid solution, and the chitosan solution was added dropwise to the esterified Poria polysaccharide solution, and the mixture was continuously stirred. The pH was adjusted to 6 with a dilute acetic acid solution, and the reaction system was placed in a constant temperature water bath at 40°C for 3 hours. The reaction mixture was transferred to a centrifuge tube, and centrifuged at 3000 rpm / min for 15 minutes using a centrifuge, the precipitate at the bottom was collected, and the precipitate was washed with deionized water, and dried in a vacuum drying oven at 40°C to obtain a modified Poria cocos complex.

[0030] S3.3. Preparation of a dehumidifying Poria composite nutrient blending oil: Grind Atractylodes macrocephala and Rhizoma Alismatis into fine powder, add 0.1% sodium hydroxide solution to moisten for 3 hours, then add ethyl acetate solvent to the mixed powder, stir and mix evenly, place in an ultrasonic cleaner, extract the components under the action of ultrasound, set the ultrasonic frequency to 35-40kHz, and the extraction time is 30-50min. After the extraction is completed, filter the extract with a Buchner funnel to remove solid residues, then transfer the filtered extract to a rotary evaporator for evaporation, and adjust the vacuum pump to 200mbar. The vacuum degree is set at 60°C, and the solvent is evaporated by heating in a water bath at 60°C until the solvent is completely evaporated to obtain the Atractylodes macrocephala and Alisma orientalis dehumidifying extract; the Atractylodes macrocephala and Alisma orientalis dehumidifying extract and the modified Poria cocos complex are fully and evenly mixed, and walnut oil, soybean oil and linseed oil are fully and evenly mixed and heated to 50°C, and then the mixed extract is divided into 5 equal portions, and added to the mixed oil in sequence, and before adding the latter portion of the extract each time, it is ensured that the previous portion of the extract has been completely dispersed, and an electric stirrer is used to stir continuously at a stirring rate of 500r / min and a stirring time of 20min to obtain a dehumidifying Poria cocos composite nutrient blending oil.

[0031] Example 2 A method for preparing a dampness-removing tuckahoe composite nutrient blended oil comprises the following steps: S3.1. Weigh the following raw materials in parts by weight: 30 parts by weight of modified Poria complex, 20 parts by weight of Atractylodes macrocephala, 10 parts by weight of Alisma orientalis, 100 parts by weight of walnut oil, 150 parts by weight of soybean oil and 200 parts by weight of linseed oil, wherein the modified Poria complex is prepared by esterification reaction of Poria polysaccharide extract with oleic acid and myristic acid in a mass ratio of 1:2:4, and then cross-linking and modification with chitosan, the mass ratio of the esterified Poria polysaccharide solution to the chitosan solution is 1:0.5, the amount of catalyst sulfuric acid added is 1% of the total mass of Poria polysaccharide extract and myristic acid, and Atractylodes macrocephala and Alisma orientalis powder are mixed with ethyl acetate in a mass ratio of 1:10.

[0032] S3.2. Preparation of modified Poria cocos complex: weigh 100 parts by weight of Poria cocos and grind it with a high-speed grinder at 10,000 r / min for 5 minutes to form powder. Add water for extraction at a mass ratio of Poria cocos powder to water of 1:8, extract at 80°C for 2 hours, filter the extract to remove solid residue, transfer the filtrate to a rotary evaporator for concentration, evaporate the water in the filtrate at 50°C, and stir the filtrate continuously until the solvent is completely evaporated to obtain Poria cocos polysaccharide extract; heat myristic acid and oleic acid to 60°C to make them molten, then add the Poria cocos polysaccharide extract, continue stirring, and add 98% by mass sulfuric acid as a catalyst, continue stirring to make it evenly dispersed, place the reaction mixture in a constant temperature water bath, set the temperature to 70°C, and continue the reaction for 6 hours. After the reaction is completed, turn off the heating source, let the mixture cool naturally to room temperature, and return to the reaction mixture. A 5% sodium hydroxide solution was added dropwise to the mixture to adjust the pH to 7, and then the neutralized mixture was added to a separatory funnel, anhydrous ethanol extraction solvent was added, and the mixture was shaken and allowed to stand for 1 hour to separate the layers, and the upper organic phase was collected. The extraction process was repeated 3 times until the extraction was complete to obtain an esterified Poria polysaccharide solution; 5 parts by weight of chitosan was weighed and dissolved in 100 mL of a 1% dilute acetic acid solution, and the chitosan solution was added dropwise to the esterified Poria polysaccharide solution, and the mixture was continuously stirred. The pH was adjusted to 6 with a dilute acetic acid solution, and the reaction system was placed in a constant temperature water bath at 40°C for 3 hours. The reaction mixture was transferred to a centrifuge tube, and centrifuged at 3000 rpm / min for 15 minutes using a centrifuge, the precipitate at the bottom was collected, and the precipitate was washed with deionized water, and dried in a vacuum drying oven at 40°C to obtain a modified Poria cocos complex.

[0033] S3.3. Preparation of a dehumidifying Poria composite nutrient blending oil: Grind Atractylodes macrocephala and Rhizoma Alismatis into fine powder, add 0.1% sodium hydroxide solution to moisten for 3 hours, then add ethyl acetate solvent to the mixed powder, stir and mix evenly, place in an ultrasonic cleaner, extract the components under the action of ultrasound, set the ultrasonic frequency to 35-40kHz, and the extraction time is 30-50min. After the extraction is completed, filter the extract with a Buchner funnel to remove solid residues, then transfer the filtered extract to a rotary evaporator for evaporation, and adjust the vacuum pump to 200mbar. The vacuum degree is set at 60°C, and the solvent is evaporated by heating in a water bath at 60°C until the solvent is completely evaporated to obtain the Atractylodes macrocephala and Alisma orientalis dehumidifying extract; the Atractylodes macrocephala and Alisma orientalis dehumidifying extract and the modified Poria cocos complex are fully and evenly mixed, and walnut oil, soybean oil and linseed oil are fully and evenly mixed and heated to 50°C, and then the mixed extract is divided into 5 equal portions, and added to the mixed oil in sequence, and before adding the latter portion of the extract each time, it is ensured that the previous portion of the extract has been completely dispersed, and an electric stirrer is used to stir continuously at a stirring rate of 500r / min and a stirring time of 20min to obtain a dehumidifying Poria cocos composite nutrient blending oil.

[0034] Example 3 A method for preparing a dampness-removing tuckahoe composite nutrient blended oil comprises the following steps: S3.1. Weigh the following raw materials in parts by weight: 30 parts by weight of modified Poria complex, 20 parts by weight of Atractylodes macrocephala, 10 parts by weight of Alisma orientalis, 100 parts by weight of walnut oil, 150 parts by weight of soybean oil and 200 parts by weight of linseed oil, wherein the modified Poria complex is prepared by esterification of Poria polysaccharide extract with oleic acid and myristic acid in a mass ratio of 1:3:5, and then cross-linking and modification with chitosan, the mass ratio of the esterified Poria polysaccharide solution to the chitosan solution is 1:0.5, the amount of catalyst sulfuric acid added is 1% of the total mass of Poria polysaccharide extract and myristic acid, and Atractylodes macrocephala and Alisma orientalis powder are mixed with ethyl acetate in a mass ratio of 1:10.

[0035] S3.2. Preparation of modified Poria cocos complex: weigh 100 parts by weight of Poria cocos and grind it with a high-speed grinder at 10,000 r / min for 5 minutes to form powder. Add water for extraction at a mass ratio of Poria cocos powder to water of 1:8, extract at 80°C for 2 hours, filter the extract to remove solid residue, transfer the filtrate to a rotary evaporator for concentration, evaporate the water in the filtrate at 50°C, and stir the filtrate continuously until the solvent is completely evaporated to obtain Poria cocos polysaccharide extract; heat myristic acid and oleic acid to 60°C to make them molten, then add the Poria cocos polysaccharide extract, continue stirring, and add 98% by mass sulfuric acid as a catalyst, continue stirring to make it evenly dispersed, place the reaction mixture in a constant temperature water bath, set the temperature to 70°C, and continue the reaction for 6 hours. After the reaction is completed, turn off the heating source, let the mixture cool naturally to room temperature, and return to the reaction mixture. A 5% sodium hydroxide solution was added dropwise to the mixture to adjust the pH to 7, and then the neutralized mixture was added to a separatory funnel, anhydrous ethanol extraction solvent was added, and the mixture was shaken and allowed to stand for 1 hour to separate the layers, and the upper organic phase was collected. The extraction process was repeated 3 times until the extraction was complete to obtain an esterified Poria polysaccharide solution; 5 parts by weight of chitosan was weighed and dissolved in 100 mL of a 1% dilute acetic acid solution, and the chitosan solution was added dropwise to the esterified Poria polysaccharide solution, and the mixture was continuously stirred. The pH was adjusted to 6 with a dilute acetic acid solution, and the reaction system was placed in a constant temperature water bath at 40°C for 3 hours. The reaction mixture was transferred to a centrifuge tube, and centrifuged at 3000 rpm / min for 15 minutes using a centrifuge, the precipitate at the bottom was collected, and the precipitate was washed with deionized water, and dried in a vacuum drying oven at 40°C to obtain a modified Poria cocos complex.

[0036] S3.3. Preparation of a dehumidifying Poria composite nutrient blending oil: Grind Atractylodes macrocephala and Rhizoma Alismatis into fine powder, add 0.1% sodium hydroxide solution to moisten for 3 hours, then add ethyl acetate solvent to the mixed powder, stir and mix evenly, place in an ultrasonic cleaner, extract the components under the action of ultrasound, set the ultrasonic frequency to 35-40kHz, and the extraction time is 30-50min. After the extraction is completed, filter the extract with a Buchner funnel to remove solid residues, then transfer the filtered extract to a rotary evaporator for evaporation, and adjust the vacuum pump to 200mbar. The vacuum degree is set at 60°C, and the solvent is evaporated by heating in a water bath at 60°C until the solvent is completely evaporated to obtain the Atractylodes macrocephala and Alisma orientalis dehumidifying extract; the Atractylodes macrocephala and Alisma orientalis dehumidifying extract and the modified Poria cocos complex are fully and evenly mixed, and walnut oil, soybean oil and linseed oil are fully and evenly mixed and heated to 50°C, and then the mixed extract is divided into 5 equal portions, and added to the mixed oil in sequence, and before adding the latter portion of the extract each time, it is ensured that the previous portion of the extract has been completely dispersed, and an electric stirrer is used to stir continuously at a stirring rate of 500r / min and a stirring time of 20min to obtain a dehumidifying Poria cocos composite nutrient blending oil.

[0037] Example 4 A method for preparing a dampness-removing tuckahoe composite nutrient blended oil comprises the following steps: S3.1. Weigh the following raw materials in parts by weight: 30 parts by weight of modified Poria complex, 20 parts by weight of Atractylodes macrocephala, 10 parts by weight of Alisma orientalis, 100 parts by weight of walnut oil, 150 parts by weight of soybean oil and 200 parts by weight of linseed oil, wherein the modified Poria complex is prepared by esterification reaction of Poria polysaccharide extract with oleic acid and myristic acid in a mass ratio of 1:3:5, and then cross-linking and modification with chitosan, the mass ratio of the esterified Poria polysaccharide solution to the chitosan solution is 1:1, the amount of catalyst sulfuric acid added is 1% of the total mass of Poria polysaccharide extract and myristic acid, and Atractylodes macrocephala and Alisma orientalis powder are mixed with ethyl acetate in a mass ratio of 1:10.

[0038] S3.2. Preparation of modified Poria cocos complex: weigh 100 parts by weight of Poria cocos and grind it with a high-speed grinder at 10,000 r / min for 5 minutes to form powder. Add water for extraction at a mass ratio of Poria cocos powder to water of 1:8, extract at 80°C for 2 hours, filter the extract to remove solid residue, transfer the filtrate to a rotary evaporator for concentration, evaporate the water in the filtrate at 50°C, and stir the filtrate continuously until the solvent is completely evaporated to obtain Poria cocos polysaccharide extract; heat myristic acid and oleic acid to 60°C to make them molten, then add the Poria cocos polysaccharide extract, continue stirring, and add 98% by mass sulfuric acid as a catalyst, continue stirring to make it evenly dispersed, place the reaction mixture in a constant temperature water bath, set the temperature to 70°C, and continue the reaction for 6 hours. After the reaction is completed, turn off the heating source, let the mixture cool naturally to room temperature, and return to the reaction mixture. A 5% sodium hydroxide solution was added dropwise to the mixture to adjust the pH to 7, and then the neutralized mixture was added to a separatory funnel, anhydrous ethanol extraction solvent was added, and the mixture was shaken and allowed to stand for 1 hour to separate the layers, and the upper organic phase was collected. The extraction process was repeated 3 times until the extraction was complete to obtain an esterified Poria polysaccharide solution; 5 parts by weight of chitosan was weighed and dissolved in 100 mL of a 1% dilute acetic acid solution, and the chitosan solution was added dropwise to the esterified Poria polysaccharide solution, and the mixture was continuously stirred. The pH was adjusted to 6 with a dilute acetic acid solution, and the reaction system was placed in a constant temperature water bath at 40°C for 3 hours. The reaction mixture was transferred to a centrifuge tube, and centrifuged at 3000 rpm / min for 15 minutes using a centrifuge, the precipitate at the bottom was collected, and the precipitate was washed with deionized water, and dried in a vacuum drying oven at 40°C to obtain a modified Poria cocos complex.

[0039] S3.3. Preparation of a dehumidifying Poria composite nutrient blending oil: Grind Atractylodes macrocephala and Rhizoma Alismatis into fine powder, add 0.1% sodium hydroxide solution to moisten for 3 hours, then add ethyl acetate solvent to the mixed powder, stir and mix evenly, place in an ultrasonic cleaner, extract the components under the action of ultrasound, set the ultrasonic frequency to 35-40kHz, and the extraction time is 30-50min. After the extraction is completed, filter the extract with a Buchner funnel to remove solid residues, then transfer the filtered extract to a rotary evaporator for evaporation, and adjust the vacuum pump to 200mbar. The vacuum degree is set at 60°C, and the solvent is evaporated by heating in a water bath at 60°C until the solvent is completely evaporated to obtain the Atractylodes macrocephala and Alisma orientalis dehumidifying extract; the Atractylodes macrocephala and Alisma orientalis dehumidifying extract and the modified Poria cocos complex are fully and evenly mixed, and walnut oil, soybean oil and linseed oil are fully and evenly mixed and heated to 50°C, and then the mixed extract is divided into 5 equal portions, and added to the mixed oil in sequence, and before adding the latter portion of the extract each time, it is ensured that the previous portion of the extract has been completely dispersed, and an electric stirrer is used to stir continuously at a stirring rate of 500r / min and a stirring time of 20min to obtain a dehumidifying Poria cocos composite nutrient blending oil.

[0040] Example 5 A method for preparing a dampness-removing tuckahoe composite nutrient blended oil comprises the following steps: S3.1. Weigh the following raw materials in parts by weight: 30 parts by weight of modified Poria complex, 20 parts by weight of Atractylodes macrocephala, 10 parts by weight of Alisma orientalis, 100 parts by weight of walnut oil, 150 parts by weight of soybean oil and 200 parts by weight of linseed oil, wherein the modified Poria complex is prepared by esterification of Poria polysaccharide extract with oleic acid and myristic acid in a mass ratio of 1:3:5, and then cross-linking and modification with chitosan, the mass ratio of the esterified Poria polysaccharide solution to the chitosan solution is 1:1.5, the amount of catalyst sulfuric acid added is 1% of the total mass of Poria polysaccharide extract and myristic acid, and Atractylodes macrocephala and Alisma orientalis powder are mixed with ethyl acetate in a mass ratio of 1:10.

[0041] S3.2. Preparation of modified Poria cocos complex: weigh 100 parts by weight of Poria cocos and grind it with a high-speed grinder at 10,000 r / min for 5 minutes to form powder. Add water for extraction at a mass ratio of Poria cocos powder to water of 1:8, extract at 80°C for 2 hours, filter the extract to remove solid residue, transfer the filtrate to a rotary evaporator for concentration, evaporate the water in the filtrate at 50°C, and stir the filtrate continuously until the solvent is completely evaporated to obtain Poria cocos polysaccharide extract; heat myristic acid and oleic acid to 60°C to make them molten, then add the Poria cocos polysaccharide extract, continue stirring, and add 98% by mass sulfuric acid as a catalyst, continue stirring to make it evenly dispersed, place the reaction mixture in a constant temperature water bath, set the temperature to 70°C, and continue the reaction for 6 hours. After the reaction is completed, turn off the heating source, let the mixture cool naturally to room temperature, and return to the reaction mixture. A 5% sodium hydroxide solution was added dropwise to the mixture to adjust the pH to 7, and then the neutralized mixture was added to a separatory funnel, anhydrous ethanol extraction solvent was added, and the mixture was shaken and allowed to stand for 1 hour to separate the layers, and the upper organic phase was collected. The extraction process was repeated 3 times until the extraction was complete to obtain an esterified Poria polysaccharide solution; 5 parts by weight of chitosan was weighed and dissolved in 100 mL of a 1% dilute acetic acid solution, and the chitosan solution was added dropwise to the esterified Poria polysaccharide solution, and the mixture was continuously stirred. The pH was adjusted to 6 with a dilute acetic acid solution, and the reaction system was placed in a constant temperature water bath at 40°C for 3 hours. The reaction mixture was transferred to a centrifuge tube, and centrifuged at 3000 rpm / min for 15 minutes using a centrifuge, the precipitate at the bottom was collected, and the precipitate was washed with deionized water, and dried in a vacuum drying oven at 40°C to obtain a modified Poria cocos complex.

[0042] S3.3. Preparation of a dehumidifying Poria composite nutrient blending oil: Grind Atractylodes macrocephala and Rhizoma Alismatis into fine powder, add 0.1% sodium hydroxide solution to moisten for 3 hours, then add ethyl acetate solvent to the mixed powder, stir and mix evenly, place in an ultrasonic cleaner, extract the components under the action of ultrasound, set the ultrasonic frequency to 35-40kHz, and the extraction time is 30-50min. After the extraction is completed, filter the extract with a Buchner funnel to remove solid residues, then transfer the filtered extract to a rotary evaporator for evaporation, and adjust the vacuum pump to 200mbar. The vacuum degree is set at 60°C, and the solvent is evaporated by heating in a water bath at 60°C until the solvent is completely evaporated to obtain the Atractylodes macrocephala and Alisma orientalis dehumidifying extract; the Atractylodes macrocephala and Alisma orientalis dehumidifying extract and the modified Poria cocos complex are fully and evenly mixed, and walnut oil, soybean oil and linseed oil are fully and evenly mixed and heated to 50°C, and then the mixed extract is divided into 5 equal portions, and added to the mixed oil in sequence, and before adding the latter portion of the extract each time, it is ensured that the previous portion of the extract has been completely dispersed, and an electric stirrer is used to stir continuously at a stirring rate of 500r / min and a stirring time of 20min to obtain a dehumidifying Poria cocos composite nutrient blending oil.

[0043] Example 6 A method for preparing a dampness-removing tuckahoe composite nutrient blended oil comprises the following steps: S3.1. Weigh the following raw materials in parts by weight: 25 parts by weight of modified Poria complex, 20 parts by weight of Atractylodes macrocephala, 10 parts by weight of Alisma orientalis, 100 parts by weight of walnut oil, 150 parts by weight of soybean oil and 200 parts by weight of linseed oil, wherein the modified Poria complex is prepared by esterification of Poria polysaccharide extract with oleic acid and myristic acid in a mass ratio of 1:3:5, and then cross-linking and modification with chitosan, the mass ratio of the esterified Poria polysaccharide solution to the chitosan solution is 1:1, the amount of catalyst sulfuric acid added is 1% of the total mass of Poria polysaccharide extract and myristic acid, and Atractylodes macrocephala and Alisma orientalis powder are mixed with ethyl acetate in a mass ratio of 1:10.

[0044] S3.2. Preparation of modified Poria cocos complex: weigh 100 parts by weight of Poria cocos and grind it with a high-speed grinder at 10,000 r / min for 5 minutes to form powder. Add water for extraction at a mass ratio of Poria cocos powder to water of 1:8, extract at 80°C for 2 hours, filter the extract to remove solid residue, transfer the filtrate to a rotary evaporator for concentration, evaporate the water in the filtrate at 50°C, and stir the filtrate continuously until the solvent is completely evaporated to obtain Poria cocos polysaccharide extract; heat myristic acid and oleic acid to 60°C to make them molten, then add the Poria cocos polysaccharide extract, continue stirring, and add 98% by mass sulfuric acid as a catalyst, continue stirring to make it evenly dispersed, place the reaction mixture in a constant temperature water bath, set the temperature to 70°C, and continue the reaction for 6 hours. After the reaction is completed, turn off the heating source, let the mixture cool naturally to room temperature, and return to the reaction mixture. A 5% sodium hydroxide solution was added dropwise to the mixture to adjust the pH to 7, and then the neutralized mixture was added to a separatory funnel, anhydrous ethanol extraction solvent was added, and the mixture was shaken and allowed to stand for 1 hour to separate the layers, and the upper organic phase was collected. The extraction process was repeated 3 times until the extraction was complete to obtain an esterified Poria polysaccharide solution; 5 parts by weight of chitosan was weighed and dissolved in 100 mL of a 1% dilute acetic acid solution, and the chitosan solution was added dropwise to the esterified Poria polysaccharide solution, and the mixture was continuously stirred. The pH was adjusted to 6 with a dilute acetic acid solution, and the reaction system was placed in a constant temperature water bath at 40°C for 3 hours. The reaction mixture was transferred to a centrifuge tube, and centrifuged at 3000 rpm / min for 15 minutes using a centrifuge, the precipitate at the bottom was collected, and the precipitate was washed with deionized water, and dried in a vacuum drying oven at 40°C to obtain a modified Poria cocos complex.

[0045] S3.3. Preparation of a dehumidifying Poria composite nutrient blending oil: Grind Atractylodes macrocephala and Rhizoma Alismatis into fine powder, add 0.1% sodium hydroxide solution to moisten for 3 hours, then add ethyl acetate solvent to the mixed powder, stir and mix evenly, place in an ultrasonic cleaner, extract the components under the action of ultrasound, set the ultrasonic frequency to 35-40kHz, and the extraction time is 30-50min. After the extraction is completed, filter the extract with a Buchner funnel to remove solid residues, then transfer the filtered extract to a rotary evaporator for evaporation, and adjust the vacuum pump to 200mbar. The vacuum degree is set at 60°C, and the solvent is evaporated by heating in a water bath at 60°C until the solvent is completely evaporated to obtain the Atractylodes macrocephala and Alisma orientalis dehumidifying extract; the Atractylodes macrocephala and Alisma orientalis dehumidifying extract and the modified Poria cocos complex are fully and evenly mixed, and walnut oil, soybean oil and linseed oil are fully and evenly mixed and heated to 50°C, and then the mixed extract is divided into 5 equal portions, and added to the mixed oil in sequence, and before adding the latter portion of the extract each time, it is ensured that the previous portion of the extract has been completely dispersed, and an electric stirrer is used to stir continuously at a stirring rate of 500r / min and a stirring time of 20min to obtain a dehumidifying Poria cocos composite nutrient blending oil.

[0046] Example 7 A method for preparing a dampness-removing tuckahoe composite nutrient blended oil comprises the following steps: S3.1. Weigh the following raw materials in parts by weight: 28 parts by weight of modified Poria complex, 20 parts by weight of Atractylodes macrocephala, 10 parts by weight of Alisma orientalis, 100 parts by weight of walnut oil, 150 parts by weight of soybean oil and 200 parts by weight of linseed oil, wherein the modified Poria complex is prepared by esterification of Poria polysaccharide extract with oleic acid and myristic acid in a mass ratio of 1:3:5, and then cross-linking and modification with chitosan, the mass ratio of the esterified Poria polysaccharide solution to the chitosan solution is 1:1, the amount of catalyst sulfuric acid added is 1% of the total mass of Poria polysaccharide extract and myristic acid, and Atractylodes macrocephala and Alisma orientalis powder are mixed with ethyl acetate in a mass ratio of 1:10.

[0047] S3.2. Preparation of modified Poria cocos complex: weigh 100 parts by weight of Poria cocos and grind it with a high-speed grinder at 10,000 r / min for 5 minutes to form powder. Add water for extraction at a mass ratio of Poria cocos powder to water of 1:8, extract at 80°C for 2 hours, filter the extract to remove solid residue, transfer the filtrate to a rotary evaporator for concentration, evaporate the water in the filtrate at 50°C, and stir the filtrate continuously until the solvent is completely evaporated to obtain Poria cocos polysaccharide extract; heat myristic acid and oleic acid to 60°C to make them molten, then add the Poria cocos polysaccharide extract, continue stirring, and add 98% by mass sulfuric acid as a catalyst, continue stirring to make it evenly dispersed, place the reaction mixture in a constant temperature water bath, set the temperature to 70°C, and continue the reaction for 6 hours. After the reaction is completed, turn off the heating source, let the mixture cool naturally to room temperature, and return to the reaction mixture. A 5% sodium hydroxide solution was added dropwise to the mixture to adjust the pH to 7, and then the neutralized mixture was added to a separatory funnel, anhydrous ethanol extraction solvent was added, and the mixture was shaken and allowed to stand for 1 hour to separate the layers, and the upper organic phase was collected. The extraction process was repeated 3 times until the extraction was complete to obtain an esterified Poria polysaccharide solution; 5 parts by weight of chitosan was weighed and dissolved in 100 mL of a 1% dilute acetic acid solution, and the chitosan solution was added dropwise to the esterified Poria polysaccharide solution, and the mixture was continuously stirred. The pH was adjusted to 6 with a dilute acetic acid solution, and the reaction system was placed in a constant temperature water bath at 40°C for 3 hours. The reaction mixture was transferred to a centrifuge tube, and centrifuged at 3000 rpm / min for 15 minutes using a centrifuge, the precipitate at the bottom was collected, and the precipitate was washed with deionized water, and dried in a vacuum drying oven at 40°C to obtain a modified Poria cocos complex.

[0048] S3.3. Preparation of a dehumidifying Poria composite nutrient blending oil: Grind Atractylodes macrocephala and Rhizoma Alismatis into fine powder, add 0.1% sodium hydroxide solution to moisten for 3 hours, then add ethyl acetate solvent to the mixed powder, stir and mix evenly, place in an ultrasonic cleaner, extract the components under the action of ultrasound, set the ultrasonic frequency to 35-40kHz, and the extraction time is 30-50min. After the extraction is completed, filter the extract with a Buchner funnel to remove solid residues, then transfer the filtered extract to a rotary evaporator for evaporation, and adjust the vacuum pump to 200mbar. The vacuum degree is set at 60°C, and the solvent is evaporated by heating in a water bath at 60°C until the solvent is completely evaporated to obtain the Atractylodes macrocephala and Alisma orientalis dehumidifying extract; the Atractylodes macrocephala and Alisma orientalis dehumidifying extract and the modified Poria cocos complex are fully and evenly mixed, and walnut oil, soybean oil and linseed oil are fully and evenly mixed and heated to 50°C, and then the mixed extract is divided into 5 equal portions, and added to the mixed oil in sequence, and before adding the latter portion of the extract each time, it is ensured that the previous portion of the extract has been completely dispersed, and an electric stirrer is used to stir continuously at a stirring rate of 500r / min and a stirring time of 20min to obtain a dehumidifying Poria cocos composite nutrient blending oil.

[0049] Comparative Example 1 The method of Example 4 was adopted, but myristic acid and oleic acid were not used to esterify Pachymaran.

[0050] Comparative Example 2 The method of Example 4 was adopted without using chitosan to modify Pachymaran.

[0051] Comparative Example 3 The method of Example 4 was adopted, and only oleic acid was used to modify Pachymaran.

[0052] Comparative Example 4 The method of Example 4 was adopted, and only myristic acid was used to modify Pachycocos polysaccharide.

[0053] The present invention discloses a method for preparing a dehumidifying Poria cocos composite nutritional blending oil prepared by using a modified Poria cocos complex, wherein the performance index inspection items and inspection standards of the method for preparing a dehumidifying Poria cocos composite nutritional blending oil are as follows: Peroxide value is an important indicator to measure the degree of oil oxidation. First, accurately weigh 5g of oil sample, put the oil sample into an iodine volumetric flask, add 30mL of a mixed solvent of chloroform and glacial acetic acid (volume ratio of 2:1), add saturated potassium iodide solution, shake gently to mix evenly, and place in the dark for 48h to promote the reaction. Prepare 0.01mol / L sodium thiosulfate standard solution as a titrant and dye it with starch indicator. Add sodium thiosulfate solution drop by drop into the solution containing the oil sample until the blue color disappears. At this time, record the volume of sodium thiosulfate solution consumed, and perform a blank test at the same time to correct the titration volume. Calculate the peroxide value of the oil sample based on the volume of sodium thiosulfate solution consumed and its concentration: POV=1000×C×(V−V0) / W, where C is the concentration of sodium thiosulfate standard solution (mol / L), V is the volume of sodium thiosulfate standard solution consumed by the sample (mL), V0 is the volume of sodium thiosulfate standard solution consumed by the blank (mL), and W is the mass of the sample (g).

[0054] When there is too much moisture, it will cause the burden on the liver to increase. Therefore, the liver function indicator ALT (alanine aminotransferase) is tested to assist in assessing the health of the liver. Blood samples are collected through the vein, placed in a test tube containing an anticoagulant, centrifuged, and the serum or plasma part is separated. An automatic biochemical analyzer is used to detect the above liver function indicators.

[0055] According to the above standards, the preparation method of a dehumidifying Poria composite nutrient blended oil prepared in the above Examples 1-5 and Comparative Examples 1-4 was tested, and the obtained data are shown in Table 1: Table 1 Performance data of Examples 1-5 and Comparative Examples 1-4 The above data fully show that compared with Comparative Examples 1-4, Examples 1-5 can fully show the effect of the modified Poria cocos complex on the method for preparing a dehumidifying Poria cocos composite nutritional blending oil in terms of dehumidification and stability.

[0056] Since the present invention adopts a modified Poria cocos complex to prepare a method for preparing a dehumidifying Poria cocos composite nutritional blending oil, the dehumidifying effect and stability of the dehumidifying Poria cocos composite nutritional blending oil are effectively improved by the modified Poria cocos complex, as follows: It can be seen from Examples 1-3 that with the continuous increase in the content of myristic acid and oleic acid, the dehumidifying effect and stability of a dehumidifying Poria composite nutritional blended oil gradually increase. This is because myristic acid and oleic acid are both long-chain fatty acids, and the hydrophobic groups of these fatty acids can be connected to the Poria polysaccharide molecules through esterification reactions. The esterified Poria polysaccharide can be better dispersed in oils and fats, thereby improving its solubility in the blended oil.

[0057] It can be seen from Examples 3-5 that with the continuous increase in chitosan content, the dehumidifying effect and stability of a dehumidifying Poria cocos composite nutritional blended oil first increase and then decrease. This is because chitosan is a natural cationic polysaccharide with good biocompatibility and film-forming properties. It forms a three-dimensional network structure by cross-linking with functional groups such as carboxyl or hydroxyl groups in Poria cocos polysaccharide, making it more stable in the blended oil. With the further increase in chitosan content, the particles of the complex become too large or aggregated, affecting its dispersibility in oils and fats. The bioavailability of the modified Poria cocos complex decreases, thereby affecting its absorption and utilization efficiency in the body, and ultimately leading to a weakened dehumidifying effect. At the same time, excessive chitosan will cause the complex to be more prone to coagulation or precipitation during storage, thereby reducing the overall stability.

[0058] It can be seen from Examples 4, 6 and 7 that with the increase in the content of the modified Poria cocos complex, the dehumidifying effect and stability of a dehumidifying Poria cocos composite nutritional blending oil gradually increase. This is because after Poria cocos polysaccharide is esterified, its solubility and absorption rate in the digestive tract are improved. The increase in the content of the modified Poria cocos complex means that more modified Poria cocos polysaccharide is involved in the digestion process, and can also better exert the dehumidifying effect.

[0059] According to the above test experiments, the preparation method of a dehumidifying Poria composite nutrient blended oil prepared according to Example 4 has the best performance, so Example 4 is taken as the best embodiment; By comparing Example 4 with Comparative Examples 1-4, it can be seen that: In Comparative Example 1, myristic acid and oleic acid are not used to esterify Poria polysaccharide, and the dehumidifying effect and stability of the preparation method of a dehumidifying Poria composite nutritional blended oil are poor. This is because myristic acid and oleic acid are both hydrophobic fatty acids, and the esterification reaction can connect hydrophobic groups to the surface of Poria polysaccharide, thereby improving its dispersibility and stability in oils and fats. Poria polysaccharide that has not been esterified has low hydrophobicity and poor dispersibility in oils and fats, and is easy to aggregate or precipitate, affecting its stability and bioavailability, and thus the dehumidifying effect and stability are deteriorated.

[0060] In Comparative Example 2, chitosan is not used to modify Poria cocos polysaccharide, and the dehumidifying effect and stability of the preparation method of a dehumidifying Poria cocos composite nutritional blended oil are poor. This is because myristic acid and oleic acid are both hydrophobic fatty acids, and the hydrophobic groups on the surface of Poria cocos polysaccharide can be connected through esterification reaction, thereby improving its dispersibility and stability in oils and fats. Poria cocos polysaccharide that has not been esterified has low hydrophobicity and poor dispersibility in oils and fats, and is easy to aggregate or precipitate, thereby affecting its stability and dehumidifying effect. In addition, unmodified Poria cocos polysaccharide may be more susceptible to external factors in the blended oil and undergo denaturation or degradation, thereby reducing the overall performance.

[0061] In comparative example 3, only oleic acid is used to modify Poria cocos polysaccharide. The dehumidifying effect and stability of the preparation method of a dehumidifying Poria cocos composite nutritional blended oil are poor. This is because oleic acid is an unsaturated fatty acid. Through esterification reaction, hydrophobic groups can be connected to the surface of Poria cocos polysaccharide, thereby improving its dispersibility and stability in oils and fats. When only oleic acid is used for modification, the dispersibility of Poria cocos polysaccharide in the blended oil is not as good as when myristic acid and oleic acid are used at the same time. Its hydrophobicity is relatively weak, which makes it insufficient for Poria cocos polysaccharide to form a sufficiently stable dispersion system in the blended oil, thereby deteriorating the dehumidifying effect and stability.

[0062] In comparative example 4, only myristic acid is used to modify Poria cocos polysaccharide, and the dehumidifying effect and stability of the preparation method of a dehumidifying Poria cocos composite nutritional blended oil are poor. This is because myristic acid and oleic acid are both fatty acids, containing a large number of carboxyl groups, which can undergo esterification reactions with the hydroxyl groups on Poria cocos polysaccharide, thereby connecting the hydrophobic groups on the surface of Poria cocos polysaccharide to improve its dispersibility and stability in oils and fats. When only myristic acid is used to modify Poria cocos polysaccharide, the substrate that can undergo esterification reaction is reduced, the substrate concentration becomes lower, and the collision probability between the reactants becomes smaller, resulting in poor reaction efficiency, less product, and reduced hydrophobicity, which in turn affects the dispersibility and stability of Poria cocos polysaccharide in the blended oil, and thus the dehumidifying effect and stability become poor.

[0063] In summary, Poria polysaccharide is first esterified with myristic acid and oleic acid to improve the hydrophobicity of Poria polysaccharide, and then cross-linked with chitosan to prepare a modified Poria complex, which enhances the stability of Poria polysaccharide in blended oil, making the dehumidifying Poria composite nutritional blended oil have the effect of dehumidifying and also have the advantage of a longer shelf life.

[0064] The above shows and describes the basic principles, main features and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited by the above embodiments, and the above embodiments and descriptions are only preferred examples of the present invention, and are not intended to limit the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, and these changes and improvements all fall within the scope of the present invention to be protected.

Claims

1. A method for preparing a dampness-removing tuckahoe composite nutrient blended oil, characterized in that: S1.1, weigh the following raw materials in parts by weight: 25-30 parts by weight of modified Poria cocos complex, 20-25 parts by weight of Atractylodes macrocephala, 10-15 parts by weight of Rhizoma Alismatis, 100-105 parts by weight of walnut oil, 150-155 parts by weight of soybean oil, 200-205 parts by weight of linseed oil, 10-20 parts by weight of sodium hydroxide solution and 30-40 parts by weight of ethyl acetate solvent; The modified Poria cocos complex is prepared by esterification of Poria cocos polysaccharide extract with oleic acid and myristic acid in a mass ratio of 1:1 to 3:3 to 5, and then cross-linking with chitosan. S1.

2. Grind Atractylodes macrocephala and Rhizoma Alismatis into fine powder, add sodium hydroxide solution with a concentration of 0.05-0.1% to moisten for 3-4 hours, then add ethyl acetate solvent to the mixed powder, stir and mix evenly, place in an ultrasonic cleaner, extract components under the action of ultrasound, after the extraction is completed, filter the extract with a Buchner funnel to remove solid residues, then transfer the filtered extract to a rotary evaporator for evaporation, adjust the vacuum pump to a vacuum degree of 100-200 mbar, and evaporate the solvent in a water bath at 50-60°C until the solvent is completely evaporated to obtain Atractylodes macrocephala and Rhizoma Alismatis dehumidifying extracts; S1.

3. Fully mix the dehumidifying extracts of Atractylodes macrocephala and Alisma orientalis with the modified Poria cocos complex, and fully mix the walnut oil, soybean oil and linseed oil and heat them to 40-50°C. Then divide the mixed extract into 5 equal parts and add them to the mixed oil in sequence. Before adding the next part of the extract each time, ensure that the previous part of the extract has been completely dispersed, and use an electric stirrer to stir continuously at a stirring rate of 500-800r / min and a stirring time of 10-20min to obtain a dehumidifying Poria cocos composite nutritious blending oil.

2. The method for preparing a dehumidifying Poria cocos composite nutrient blended oil according to claim 1, characterized in that: In the S1.2, the Atractylodes macrocephala and Alismatis rhizome powders are mixed with ethyl acetate in a mass ratio of 1:10 to 12.

3. The method for preparing a dehumidifying Poria cocos composite nutrient blended oil according to claim 1, characterized in that: In S1.2, the ultrasonic frequency is set to 35-40 kHz, and the extraction time is 30-50 min.

4. The method for preparing a dehumidifying Poria cocos composite nutrient blended oil according to claim 1, characterized in that: The steps for preparing the modified Poria cocos composite are specifically as follows: S2.1, weigh 100 parts by weight of Poria cocos and grind it into fine powder with a high-speed grinder, add water for extraction, extract at 80°C-100°C for 1-2h, filter the extract, remove solid residue, transfer the filtrate to a rotary evaporator for concentration, evaporate the water in the filtrate at 50-60°C, and stir the filtrate continuously until the solvent is completely evaporated to obtain Poria cocos polysaccharide extract; S2.2, heat myristic acid and oleic acid to 60-65 ° C to make them molten, then add the Poria polysaccharide extract, continue stirring, and add sulfuric acid as a catalyst, continue stirring to make it evenly dispersed, place the reaction mixture in a constant temperature water bath, set the temperature to 60-80 ° C, and continue the reaction for 6-8 hours. After the reaction is completed, turn off the heating source, let the mixture cool naturally to room temperature, add sodium hydroxide solution dropwise to the reaction mixture to adjust the pH, then add the neutralized mixture to a separatory funnel, add anhydrous ethanol extraction solvent, shake and let stand for 1-2 hours to separate, collect the upper organic phase, repeat the extraction process 2-3 times until the extraction is complete, and obtain an esterified Poria polysaccharide solution; S2.

3. Weigh 5 parts by weight of chitosan and dissolve it in 100 mL of dilute acetic acid solution, and add the chitosan solution drop by drop into the esterified Poria polysaccharide solution while stirring continuously. Adjust the pH to 5-6 with dilute acetic acid solution, and place the reaction system in a constant temperature water bath at 30-40°C for 2-3 hours. Transfer the reaction mixture to a centrifuge tube, centrifuge it at 3000-5000 rpm / min for 10-15 minutes, collect the precipitate at the bottom, wash the precipitate with deionized water, and dry it in a vacuum drying oven at 40-50°C to obtain a modified Poria complex.

5. The method for preparing a dehumidifying Poria cocos composite nutrient blended oil according to claim 4, characterized in that: In S2.1, the rotation speed of the high-speed pulverizer is set to 10000-20000 r / min, and the pulverizing time is 1-5 min.

6. The method for preparing the dampness-removing Poria cocos composite nutrient blended oil according to claim 4, characterized in that: In the above S2.1, the Poria cocos powder and water are mixed in a mass ratio of 1:8 to 10.

7. The method for preparing a dehumidifying Poria cocos composite nutrient blended oil according to claim 4, characterized in that: In S2.2, the mass fraction of the catalyst sulfuric acid is 96-98%, and the added amount is 1%-5% of the total mass of the Poria polysaccharide extract and myristic acid.

8. The method for preparing a dehumidifying Poria cocos composite nutrient blended oil according to claim 4, characterized in that: In S2.2, the mass fraction of the sodium hydroxide solution is 5%-10%, and the pH value is adjusted to 6-7.

9. The method for preparing a dehumidifying Poria cocos composite nutrient blended oil according to claim 4, characterized in that: In S2.3, the mass fraction of the dilute acetic acid solution is 1-5%.

10. The method for preparing a dehumidifying Poria cocos composite nutrient blended oil according to claim 4, characterized in that: In the S2.3, the mass ratio of the esterified pachymaran solution to the chitosan solution is 1:0.5-1.5.

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