Preparation method of sambucus williamsii extract for cigarettes, sambucus williamsii extract for cigarettes and application

Abstract

The invention discloses a preparation method of a sambucus williamsii extract for cigarettes. The preparation method comprises the following steps: S1, crushing, S2, dissolving, S3, enzymolysis, S4, reflux extraction, and S5, reduced pressure distillation and concentration. The method has the advantages that the raw materials are treated by adopting an enzymolysis technology, so that the content of plant cells is easy to flow out, and the extraction efficiency can be improved; based on the characteristics of sambucus williamsii polyphenol, the sambucus williamsii polyphenol also plays a certain role in reducing free radicals in smoke and reducing harm to a human body; maillard reaction is also involved in the extraction process, most of heterocyclic compounds generated in the reaction process are also important aroma components in tobacco, and when a proper amount of the obtained product is added into cigarettes, the effects of coordinating cigarette aroma, improving smoke concentration, making smoke delicate and soft, reducing irritation and improving aroma quality are achieved.

Description

Technical Field

[0001] The invention relates to the technical field of tobacco additives, and in particular to a preparation method of an elderberry extract for tobacco, the elderberry extract for tobacco and applications thereof. Background Art

[0002] Elderberry (Sambucus nigra L.) is the fruit of the elderberry plant of the genus Sambucus in the family Caprifoliaceae. The whole plant can be used as medicine, which has the effects of dispelling wind and dampness, promoting blood circulation, and stopping bleeding. The main production areas of elderberry are Jiangsu, Hubei, Zhejiang, Fujian, Hunan and other places. The earliest record of elderberry was found in "Tang Materia Medica" and "Lu Canyan Materia Medica". Elderberry has attracted people's attention in ancient times. Its medicinal effects are recorded in classics such as "Compendium of Materia Medica" and "Sui Xi Ju Diet". Elderberry has very high nutritional value. Traditional Chinese medicine believes that elderberry is cool in nature and bitter in taste. It has the effects of clearing heat and calming the mind, treating constipation, nourishing the heart and brain, lowering blood pressure, retaining fluid and stopping sweating, and stopping bleeding and consolidating essence.

[0003] Cigarette harm reduction and tar reduction is a major trend in the development of China's tobacco industry and the main task of the development of China's tobacco industry. The work of reducing harm and reducing tar in cigarettes cannot be separated from the promotion of scientific and technological innovation, which is the core support for the solid and steady progress of the work of reducing harm and reducing tar in cigarettes. Similarly, for cigarette industrial enterprises, doing a good job in scientific and technological innovation in reducing harm and reducing tar in cigarettes will also help independent brands to establish new competitive advantages by virtue of their leading advantages in reducing harm and reducing tar in cigarettes. Studies have shown that the elderberry polyphenols contained in elderberry can be added to tobacco and filter rods to effectively eliminate free radicals in cigarette smoke, thereby reducing the harm to the human body of smokers.

[0004] In recent years, there have been many reports on the preparation of tobacco flavors from extracts of natural products using enzymatic hydrolysis technology and Maillard reaction. These technologies can not only improve the quality of smoke, but also increase the utilization rate of raw materials. The Maillard reaction products contain a large number of aroma components that are coordinated with tobacco, and have the advantages of strong natural feeling and full aroma. They have achieved good application results in improving the quality of tobacco products. Therefore, the Maillard reaction has become an important way to prepare tobacco flavors.

[0005] At present, elderberry extracts are widely used in cosmetics. They have the effects of moisturizing the skin and beautifying. The elderberry glycosides and mucilage have bactericidal, anti-inflammatory and antipruritic functions. They can also be used in shampoo and hair care daily necessities. At the same time, they are also used in the pharmaceutical industry because of their pharmacological effects. At present, there are few studies on the application of elderberry extracts in traditional cigarette additives. Summary of the invention

[0006] The technical problem to be solved by the present invention is to provide a method for preparing an elderberry extract for cigarettes, wherein the elderberry extract is obtained by crushing elderberry raw materials, enzymolysis, reflux extraction (Maillard reaction), and vacuum distillation concentration. The present invention prepares the elderberry extract by an enzyme treatment method, and also spontaneously performs Maillard reaction through natural plant raw materials, so that the prepared elderberry extract is more suitable for traditional cigarettes. When added to cigarettes, it has the effects of coordinating the smoke aroma, increasing smoke concentration, making smoke delicate and soft, reducing stimulation and improving aroma quality.

[0007] In order to solve the above technical problems, the technical solution of the present invention is:

[0008] A method for preparing an elderberry extract for tobacco comprises the following steps:

[0009] S1, crushing step: crushing the elderberry raw material and passing it through a 20-mesh sieve to obtain elderberry raw material powder;

[0010] S2, dissolving step: adding water to dissolve the elderberry raw material powder to obtain solution A;

[0011] S3, enzymatic hydrolysis step: dissolving cellulase, pectinase and hydrolase in water to prepare an enzymatic hydrolysis solution, adding the enzymatic hydrolysis solution to solution A and reacting at 35°C to 50°C for 3 to 5 hours;

[0012] S4, reflux extraction step: heating the enzymatic hydrolysis product at 110° C. to 125° C. for reflux extraction for 1.5 to 3 hours, filtering to obtain filtrate B, adding 50% to 70% ethanol solution to the filter residue, reflux extraction at 60° C. to 70° C. for 1.5 to 3 hours to obtain filtrate C, and combining filtrate B with filtrate C to obtain elderberry extract;

[0013] S5, a vacuum distillation and concentration step: taking the elderberry extract and performing vacuum distillation and concentration to obtain the desired elderberry extract for tobacco.

[0014] As a preferred embodiment of the method for preparing the elderberry extract for tobacco of the present invention, the mass ratio of the coarse powder to water in step S2 is 1:(5-10).

[0015] As a preferred embodiment of the method for preparing the elderberry extract for tobacco of the present invention, the mass ratio of the filter residue to water in step S4 is 1:(5-10).

[0016] As a preferred embodiment of the method for preparing the elderberry extract for tobacco of the present invention, the mass ratio of cellulase, pectinase, hydrolase and water in the enzymatic hydrolysis solution of step S3 is (1-3):(1-3):(1-6):10.

[0017] As a preferred embodiment of the method for preparing the elderberry extract for tobacco of the present invention, the hydrolase is prepared by compounding amylase, papain, pepsin and neutral protease in a mass ratio of (1-3):(1-3):(1-3):(1-3).

[0018] As a preferred embodiment of the method for preparing the elderberry extract for tobacco of the present invention, the amount of the enzymatic hydrolysis solution added is 0.1% to 0.3% of the mass of solution A.

[0019] As a preferred embodiment of the method for preparing the elderberry extract for tobacco of the present invention, the Maillard reaction is carried out while heating and refluxing the extract in step S4, and the reaction conditions are 110° C. to 125° C. for 1.5 to 3 hours, and the enzyme is inactivated in step S4.

[0020] As a preferred embodiment of the method for preparing the elderberry extract for tobacco of the present invention, the pressure of the reduced pressure distillation concentration in step S5 is -0.05MPa to -0.1MPa, and the temperature is 60°C to 70°C.

[0021] A tobacco elderberry extract is obtained by the preparation method.

[0022] An application of the elderberry extract for tobacco obtained by the preparation method, wherein the elderberry extract for tobacco is applied to a traditional tobacco leaf group formula as an additive, and the added amount is 0.1-0.5% of the leaf group mass.

[0023] Beneficial effects of the present invention:

[0024] The invention adopts enzymatic hydrolysis technology to process raw materials, which is beneficial to the outflow of plant cell contents and can improve the extraction efficiency; based on the characteristics of elderberry polyphenol, it also has a certain effect on reducing free radicals in smoke and reducing harm to human body; the extraction process also involves Maillard reaction, and most of the heterocyclic compounds generated in the reaction process are also important aroma components in tobacco. The obtained product is added to cigarettes in an appropriate amount, which has the effects of coordinating the aroma of tobacco, increasing the smoke concentration, making the smoke delicate and soft, reducing stimulation and improving the aroma quality. DETAILED DESCRIPTION

[0025] The specific embodiments of the present invention are further described below. It should be noted that the description of these embodiments is used to help understand the present invention, but does not constitute a limitation of the present invention. In addition, the technical features involved in the various embodiments of the present invention described below can be combined with each other as long as they do not conflict with each other.

[0026] Example 1

[0027] A method for preparing an elderberry extract for tobacco comprises the following steps:

[0028] S1, crushing step: crushing the elderberry raw material and passing it through a 20-mesh sieve to obtain elderberry raw material powder;

[0029] S2, dissolving step: adding 5 times the weight of water to the elderberry raw material powder to dissolve it to obtain solution A;

[0030] S3, enzymatic hydrolysis step: cellulase, pectinase, hydrolase and water are mixed in a mass ratio of 1:1:3:10 to prepare an enzymatic solution, wherein the hydrolase is prepared by compounding amylase, papain, pepsin and neutral protease in a mass ratio of 2:1:1:2, and the enzymatic solution is added to solution A and reacted at 45°C for 3 hours, and the amount of the enzymatic solution added is 0.1% of the mass of solution A;

[0031] S4, reflux extraction step: heating the enzymatic hydrolysis product at 115° C. for reflux extraction for 2 h, filtering to obtain filtrate B, adding 5 times the mass of 50% ethanol solution to the filter residue, reflux extraction at 65° C. for 3 h to obtain filtrate C, and combining filtrate B with filtrate C to obtain elderberry extract;

[0032] S5, vacuum distillation and concentration step: the elderberry extract is subjected to vacuum distillation and concentration at a pressure of -0.1 MPa and a temperature of 60° C. to obtain the desired elderberry extract D1 for tobacco use.

[0033] Example 2

[0034] A method for preparing an elderberry extract for tobacco comprises the following steps:

[0035] S1, crushing step: crushing the elderberry raw material and passing it through a 20-mesh sieve to obtain elderberry raw material powder;

[0036] S2, dissolving step: adding 10 times the weight of water to the elderberry raw material powder to dissolve it to obtain solution A;

[0037] S3, enzymatic hydrolysis step: cellulase, pectinase, hydrolase and water are mixed in a mass ratio of 1:1:3:10 to prepare an enzymatic solution, wherein the hydrolase is prepared by compounding amylase, papain, pepsin and neutral protease in a mass ratio of 2:1:1:2, and the enzymatic solution is added to solution A and reacted at 50° C. for 5 hours, wherein the amount of the enzymatic solution added is 0.3% of the mass of solution A;

[0038] S4, reflux extraction step: heating the enzymatic hydrolysis product at 125° C. for reflux extraction for 3 h, filtering to obtain filtrate B, adding 10 times the mass of 50% ethanol solution to the filter residue, reflux extraction at 70° C. for 3 h to obtain filtrate C, and combining filtrate B with filtrate C to obtain elderberry extract;

[0039] S5, vacuum distillation and concentration step: the elderberry extract is subjected to vacuum distillation and concentration at a pressure of -0.1 MPa and a temperature of 60° C. to obtain the desired elderberry extract D2 for tobacco use.

[0040] Comparative Example 1

[0041] No enzyme treatment was performed, no step S3 was performed, and the other steps were the same as in Example 1 to obtain the desired elderberry extract D3 for tobacco.

[0042] Comparative Example 2

[0043] In step S3, no hydrolase is added, and the other steps are the same as in Example 1, to obtain the desired elderberry extract D4 for tobacco.

[0044] The elderberry extracts prepared in the above examples and comparative examples were used to conduct sensory quality evaluation experiments:

[0045] The elderberry extracts D1, D2, D3, and D4 obtained above were added to the prepared tobacco leaf group according to the conventional adding process at an amount of 0.1% of the dry weight of the tobacco shreds by the mass of the extracts, and then a group of blank controls without additions were set up to make cigarettes, and then 10 professional sensory evaluators were randomly selected to conduct sensory quality evaluation on the cigarettes obtained in each example. Among them, the sensory quality of the cigarettes of the present invention refers to the cigarette sensory quality evaluation standard in "GB 5606.42005 Cigarettes Part 4: Sensory Technical Requirements", and the professional sensory evaluators scored the sensory quality of each example cigarette, and then took the average value as the final score. The sensory quality evaluation score results of each example cigarette are shown in Table 1:

[0046]

[0047] Table 1

[0048] The sensory evaluation results of the above-mentioned example cigarettes were compared and smoked as shown in Table 2:

[0049]

[0050] Table 2

[0051] From the data in Table 1, it can be seen that the elderberry extract for tobacco prepared by the preparation method of the present invention has a significant improvement in sensory quality compared with the unadded leaf group. By comparing with Comparative Example 1 which has not been treated with enzymes, it can be seen that the elderberry extract obtained by enzyme treatment has significant improvements in cigarette aroma and coordination. Comparative Example 2 shows that the examples in which the Maillard reaction is performed have a certain effect on improving the aroma quality of cigarettes.

[0052] From the evaluation results in Table 2, it can be seen that the elderberry extract for tobacco prepared by the present invention has the effects of coordinating tobacco aroma, increasing smoke concentration, making smoke delicate and soft, reducing irritation and improving aroma quality. In summary, through the evaluation results of 10 professional sensory evaluation personnel, it can be seen that the elderberry extract prepared by the preparation method mentioned in the present invention is applied to cigarette additives, which has the effects of coordinating tobacco aroma, increasing smoke concentration, making smoke delicate and soft, reducing irritation and improving aroma quality.

[0053] The embodiments of the present invention are described in detail above, but the present invention is not limited to the described embodiments. For those skilled in the art, various changes, modifications, substitutions and variations of these embodiments are made without departing from the principles and spirit of the present invention, and still fall within the protection scope of the present invention.

Claims

1. A method for preparing an elderberry extract for tobacco, characterized in that: The following steps are involved: S1, crushing step: crushing the elderberry raw material and passing it through a 20-mesh sieve to obtain elderberry raw material powder; S2, dissolving step: adding water to dissolve the elderberry raw material powder to obtain solution A; S3, enzymatic hydrolysis step: dissolving cellulase, pectinase and hydrolase in water to prepare an enzymatic hydrolysis solution, adding the enzymatic hydrolysis solution to solution A and reacting at 35°C to 50°C for 3 to 5 hours; S4, reflux extraction step: heating the enzymatic hydrolysis product at 110° C. to 125° C. for reflux extraction for 1.5 to 3 hours, filtering to obtain filtrate B, adding 50% to 70% ethanol solution to the filter residue, reflux extraction at 60° C. to 70° C. for 1.5 to 3 hours to obtain filtrate C, and combining filtrate B with filtrate C to obtain elderberry extract; S5, a vacuum distillation and concentration step: taking the elderberry extract and performing vacuum distillation and concentration to obtain the desired elderberry extract for tobacco.

2. The method for preparing the elderberry extract for tobacco according to claim 1, characterized in that: The mass ratio of the coarse powder to water in step S2 is 1:(5-10).

3. The method for preparing the elderberry extract for tobacco according to claim 1, characterized in that: The mass ratio of the filter residue to water in step S4 is 1:(5-10).

4. The method for preparing the elderberry extract for tobacco according to claim 1, characterized in that: The mass ratio of cellulase, pectinase, hydrolase and water in the enzymatic solution of step S3 is (1-3):(1-3):(1-6):

10.

5. The method for preparing the elderberry extract for tobacco according to claim 4, characterized in that: The hydrolase is prepared by compounding amylase, papain, pepsin and neutral protease in a mass ratio of (1-3):(1-3):(1-3):(1-3).

6. The method for preparing the elderberry extract for tobacco according to claim 4, characterized in that: The amount of the enzymatic solution added is 0.1% to 0.3% of the mass of solution A.

7. The method for preparing the elderberry extract for tobacco according to claim 1, characterized in that: In the step S4, the Maillard reaction is carried out while heating and refluxing for extraction. The reaction conditions are 110° C. to 125° C. for 1.5 to 3 hours. At the same time, the enzyme is inactivated in step S4.

8. The method for preparing the elderberry extract for tobacco according to claim 1, characterized in that: The pressure of the reduced pressure distillation concentration in step S5 is -0.05MPa to -0.1MPa, and the temperature is 60°C to 70°C.

9. An elderberry extract for tobacco, characterized in that: An elderberry extract for tobacco obtained by the preparation method described in any one of claims 1 to 8.

10. An application of an elderberry extract for tobacco obtained by the preparation method of any one of claims 1 to 8, characterized in that: The elderberry extract for tobacco is applied to traditional tobacco leaf group formula and added in an amount of 0.1-0.5% of the leaf group mass.

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