Plant nourishing and repairing composition and application thereof

Abstract

The invention discloses a plant nourishing and repairing composition and application thereof, the plant nourishing and repairing composition is composed of pomegranate seed oil, tomato fruit oil and safflower oleosome, and the mass ratio of the pomegranate seed oil to the tomato fruit oil to the safflower oleosome is 1: (0.25-3): (1-4). The plant nourishing and repairing composition provided by the invention has excellent anti-ultraviolet performance and synergistic anti-oxidation performance, meanwhile, the combing performance of hair is improved, and damaged hair is nourished and repaired. The addition of the safflower oleosome enables the vegetable oil to form oil drops with uniform particle sizes, so that the vegetable oil can be uniformly dispersed in the product, and the stability of the vegetable oil is improved. The composition can be applied to a cosmetic formula and has the effects of nourishing, repairing, resisting ultraviolet damage and resisting oxidation, meanwhile, the preparation method is simple and convenient, and the composition has wide market application prospects.

Description

Technical Field

[0001] The invention belongs to the technical field of cosmetics, and in particular relates to a plant nourishing and repairing composition and application thereof. Background Art

[0002] Hair is a fiber material with keratin as the main component, and also contains lipids, water, melanin, etc. It has a complex structure from microscopic to macroscopic. There are many chemical bonds and non-bonding forces inside the hair, such as peptide bonds, disulfide bonds, hydrogen bonds, salt bonds, van der Waals forces, etc., which directly determine the performance of the hair. Many factors in daily life can cause damage to the hair. For example, daily combing can cause a slight loss of lipid structure on the surface of the hair, causing the hair to become frizzy; hair styling processes such as dyeing and perming can break the chemical bonds in the hair and cause a large loss of lipid structure in the hair, causing the hair to become prone to splitting and breaking; environmental factors such as ultraviolet rays and environmental pollutants can cause the breakage of hair chemical bonds and lead to the degradation of components such as amino acids, lipid structures and pigments, making the hair fragile, rough and even faded.

[0003] For the repair of damaged hair, existing products mainly use synthetic ingredients such as cationic surfactants and silicone oil to replenish hair surface lipids or form a film on the hair surface to reduce the loss of hair lipids. There are relatively few products that can repair damaged hair while resisting UV damage and scavenging oxygen free radicals.

[0004] Vegetable oil is extracted from seeds, fruits, germs and other parts of plants through processes such as pressing or leaching. Its main components are fatty acid glycerides formed by different types of fatty acids, and it also contains active ingredients such as tocopherol, polyphenols, and phytosterols. However, directly adding vegetable oils to product formulas may cause problems such as easy oxidation of the oils and poor compatibility, thereby limiting their actual effectiveness. Summary of the invention

[0005] The purpose of this section is to summarize some aspects of embodiments of the present invention and to briefly introduce some preferred embodiments.

[0006] As one aspect of the present invention, the present invention provides a plant nourishing and repairing composition, wherein: the plant nourishing and repairing composition is composed of pomegranate seed oil, tomato fruit oil and safflower oleosomes, and the mass ratio of the pomegranate seed oil, tomato fruit oil and safflower oleosomes is 1: (0.25~3): (1~4).

[0007] As a preferred embodiment of the plant nourishing and repairing composition of the present invention, the mass ratio of pomegranate seed oil, tomato fruit oil and safflower oleosome is 1:(0.25-2):(2-3).

[0008] As a preferred embodiment of the plant nourishing and repairing composition of the present invention, the mass ratio of pomegranate seed oil, tomato fruit oil and safflower oleosome is 1:(0.25-1):(2-3).

[0009] As a preferred embodiment of the plant nourishing and repairing composition of the present invention: the preparation method of the plant nourishing and repairing composition includes first mixing pomegranate seed oil and tomato fruit oil evenly, and then adding safflower oleosomes to mix evenly; or first mixing pomegranate seed oil and safflower oleosomes evenly, and then adding tomato fruit oil to mix evenly.

[0010] Beneficial effects of the present invention: The plant nourishing and repairing composition provided by the present invention has excellent anti-ultraviolet performance and synergistic antioxidant performance, while improving the combing properties of hair and nourishing and repairing damaged hair. The addition of safflower oleosomes allows the vegetable oil to form oil droplets with uniform particle size, which helps to evenly disperse the vegetable oil in the product and improve the stability of the vegetable oil. The composition of the present invention can be applied to cosmetic formulas to play the role of nourishing and repairing, anti-ultraviolet damage and anti-oxidation. At the same time, the preparation method is simple and convenient, and has broad market application prospects. BRIEF DESCRIPTION OF THE DRAWINGS

[0011] In order to more clearly illustrate the technical solution of the embodiment of the present invention, the following briefly introduces the drawings required for describing the embodiment, wherein:

[0012] Figure 1 UV absorption curves of different samples.

[0013] Figure 2 DPPH free radical scavenging ability of different vegetable oils.

[0014] Figure 3 Microscope images of different samples.

[0015] Figure 4 This is the test result of hair protein content. DETAILED DESCRIPTION

[0016] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the specific implementation methods of the present invention are described in detail below in conjunction with specific embodiments.

[0017] Pomegranate seed oil (CAS No.: 84961-57-9, EC No.: 284-646-0) and tomato fruit oil (EC No.: 290-375-9) are plant oil components derived from seeds or fruits. They contain saturated fatty acids such as hexadecanoic acid and octadecanoic acid, as well as unsaturated fatty acids such as oleic acid and linoleic acid. They also contain active ingredients such as tocopherol, polyphenols, and phytosterols. Both can be obtained through conventional commercial channels. The INCI name of pomegranate seed oil is pomegranate (PUNICA GRANATUM) extract or pomegranate (PUNICAGRANATUM) seed extract or pomegranate (PUNICA GRANATUM) seed oil. The INCI name of tomato fruit oil is tomato (SOLANUM LYCOPERSICUM) extract or tomato (SOLANUM LYCOPERSICUM) fruit extract or tomato (SOLANUM LYCOPERSICUM) fruit oil.

[0018] Safflower oleosomes are a mixture of lipid components isolated and extracted from safflower (Carthamus tinctorius L.) seeds, extracted by the method in reference (Karefyllakis D, Jan van der Goot A, Nikiforidis CV. The behavior of sunflower oleosomes at the interfaces. Soft Matter 2019; 15: 4639-4646.), or obtained through conventional commercial channels. The structure of safflower oleosomes includes an outer membrane composed of a single layer of phospholipids and oil body proteins and an inner core with triacylglycerol as the main component, which can be used as a natural emulsifier to stabilize lipid components. The INCl name of safflower oleosomes is safflower (CARTHAMUS TINCTORIUS) oleosomes.

[0019] Anti-ultraviolet properties of vegetable oil:

[0020] The vegetable oils to be tested were dissolved in ethanol and diluted to appropriate concentrations. Using ethanol as a blank, a UV-visible spectrophotometer was used to perform a spectral scan of the vegetable oil ethanol solution in the wavelength range of 280-400nm. At the same time, a typical sunscreen agent, ethylhexyl methoxycinnamate (OMC), was used as a control. During the experiment, it was found that the UV-visible absorption peaks of 0.01% OMC and 0.1% and 0.01% pomegranate seed oil had a large number of burrs and could not form a smooth curve. Therefore, these two samples were further diluted with ethanol to a solution with a mass fraction of 0.001% and then tested. The UV absorption curves of OMC and 9 kinds of vegetable oils are shown in Figure 2. Figure 1 shown.

[0021] The ultraviolet rays in sunlight are mainly composed of UVA (320-400nm) and UVB (280-320nm). Studies have shown that UVB causes the most serious damage to hair. It can cause the disulfide bonds in the hair to break and the tryptophan to degrade, resulting in poor appearance of hair, such as dryness, breakage, split ends, and frizziness. Figure 1 It can be seen that pomegranate seed oil has strong ultraviolet absorption in the wavelength range of 280-300nm, and the absorption peak appears near 285nm. Tomato fruit oil and palm fruit oil have certain ultraviolet absorption capacity in the wavelength range of 280-400nm. Rosehip oil and olive fruit oil only show ultraviolet absorption capacity in the wavelength range of 280-300nm; the ultraviolet absorption curves of the four vegetable oils, sea buckthorn fruit oil, white meadowfoam seed oil, sweet almond oil and milk thistle seed oil, have no obvious changes in the wavelength range of 280-400nm, and the overall absorption capacity remains at a weak level.

[0022] Antioxidant properties of vegetable oils:

[0023] 1,1-Diphenyl-2-trinitrophenylhydrazine (DPPH) is a dark purple crystal that appears dark purple in anhydrous ethanol solution. The DPPH free radical has a strong absorption peak at 517nm in the visible light region. When a free radical scavenger is present, the DPPH free radical pairs with the single electron it provides, causing the characteristic purple color to disappear and turn into colorless or light yellow. Since the degree of fading of the DPPH ethanol solution is linearly related to the number of electrons it receives, the absorbance value before and after the reaction is measured by a UV-visible spectrophotometer, thereby evaluating the antioxidant capacity of the sample.

[0024] Dilute the sample to be tested with anhydrous ethanol to a suitable concentration, transfer 5 ml of sample solution with different mass concentrations and 0.03 mg / mL DPPH ethanol solution to a 15 ml centrifuge tube, mix well and store in the dark for 30 minutes, and measure the absorbance A at 517 nm. j ; In the same way, 5 ml of sample solution and anhydrous ethanol were transferred to a 15 ml centrifuge tube, mixed evenly and then the absorbance A0 at 517 nm was measured; then 5 ml of anhydrous ethanol and 0.03 mg / mL DPPH ethanol solution were transferred to a 15 ml centrifuge tube, mixed evenly and then the absorbance A0 at 517 nm was measured. i . Calculate the DPPH free radical scavenging rate according to the formula: scavenging rate = [1-(A j -A0) / A j ]×100%. CompuSyn software was used for data processing to calculate the half clearance IC50 values ​​of different samples. The IC50 values ​​of different vegetable oils are as follows: Figure 2 shown.

[0025] IC 50The smaller the value, the stronger the antioxidant capacity of the sample. Figure 2 The DPPH free radical scavenging ability of different vegetable oils is Figure 2 It can be seen that the antioxidant capacity of the six vegetable oils is ranked from high to low as pomegranate seed oil > tomato fruit oil > sea buckthorn fruit oil > olive fruit oil > sweet almond oil > meadowfoam seed oil.

[0026] Safflower oleosomes aid in stabilization test:

[0027] Different test conditioner formulas were prepared according to the formula in Table 1, wherein the composite vegetable oil (pomegranate seed oil: tomato fruit oil mass ratio = 3:1) in the composite vegetable oil group was added directly, and the composite vegetable oil in the composite vegetable oil + oleosome group was mixed evenly with safflower oleosomes in proportion (conventional stirring can be used) and then added.

[0028] Table 1 Test formula

[0029]

[0030] The sample to be tested was placed in a 40°C oven for 24 hours and then taken out. After returning to room temperature, the sample state was observed under a 400x microscope, and pictures were taken and compared.

[0031] Figure 3 Microscope images of different samples, from Figure 3 It can be seen that the oil droplets in the samples without safflower oleosomes are of different sizes and the distribution is also more chaotic, while the samples with safflower oleosomes show a more uniform dispersion of oil droplets. This shows that safflower oleosomes help promote the uniform dispersion of plant oil components in the product, thereby improving the stability of plant oil in the system.

[0032] Synergistic antioxidant test of the composition:

[0033] Based on the measured data of anti-ultraviolet and antioxidant properties of single vegetable oils, pomegranate seed oil and tomato fruit oil were selected as vegetable oils, and safflower oleosomes were added to stabilize these two lipid components.

[0034] First, pomegranate seed oil and safflower oleosomes were mixed in a mass ratio of 1:3 to form a pomegranate seed oil / safflower oleosome combination (conventional stirring and mixing can be performed), and then tomato fruit oil was added in a mass ratio of (1:0.25), (1:0.33), (1:0.5), (1:1.0), and (1:2.0) to pomegranate seed oil and mixed evenly (conventional stirring and mixing can be performed). The proportions of the ingredients in each experimental group are shown in Table 2.

[0035] Table 2 Mass ratio of the composition components in each experimental group

[0036]

[0037] Dilute the above experimental samples with absolute ethanol to 1.0 mg / mL, 5.0 mg / mL, and 10.0 mg / mL, then measure the DPPH free radical scavenging rate of the sample solutions at different mass concentrations, and use CompuSyn software to analyze and calculate the combination index CI value. A CI value > 1 indicates antagonism, a CI value = 1 indicates additivity, 0.1 < CI value < 0.3 indicates strong synergism, and 0.3 < CI value < 0.7 indicates relatively strong synergism. The DPPH free radical scavenging rates of different samples are shown in Table 3.

[0038] Table 3 DPPH free radical scavenging ability of different samples

[0039]

[0040]

[0041] As shown in Table 3, when pomegranate seed oil and tomato fruit oil are used in combination in the range of mass ratio (1:0.25) to (1:2), the CI values are all less than 0.7, showing a synergistic antioxidant effect.

[0042] Repair test of the composition:

[0043] The combing work refers to the work done during the process of combing hair, and the size of the combing work is closely related to the state of the hair. The cutin scales on the surface of damaged hair will become rough and翘起, resulting in an increase in the friction of the hair. Correspondingly, greater resistance needs to be overcome during combing, and the combing work is greater. By comparing the changes in the combing work of damaged hair before and after using the sample, the ability of the sample to improve the combability of hair can be evaluated, and further the repair effect on damaged hair can be evaluated.

[0044] Prepare different samples according to the formula in Table 4, and the components in the table are calculated by mass percentage.

[0045] Table 4 Test formula

[0046]

[0047] Randomly take several hair bundles for damage treatment, then wash them clean with 1% sodium dodecyl sulfate aqueous solution and hang them to dry. Fix the dried hair bundles on the fixture of the combing instrument, and measure the combing work (denoted as M) of each hair bundle before using the sample. Then use the same method to treat the hair bundles with an equal amount of the sample. After the hair bundles are dried, measure the combing work (denoted as M2) after using the sample again. Each hair bundle is combed 5 times repeatedly, and 5 sets of parallel data are taken to calculate the average value. Analyze the test data by calculating the change rate ΔM of the combing work. The calculation formula of the change rate ΔM of the combing work is as follows:

[0048]

[0049] In the formula, ΔM represents the change rate of the combing power of the hair bundle before and after the use of the sample. The larger the ΔM value, the better the sample's ability to improve the combing property of the hair bundle, that is, the better the repair effect on damaged hair. The test results of the combing property of the samples in Table 4 are shown in Table 5.

[0050] Table 5 Combing test results

[0051]

[0052] The data in the table are all before and after use, * indicates p < 0.05, that is, the data has significant differences. As can be seen from Table 5, any one of the ingredients in the composition can reduce the combing work of hair when used alone, indicating that they all have a certain repair effect. However, after the three are compounded, the improvement effect on combing is significantly improved, indicating that the combination of pomegranate seed oil, tomato fruit oil, and safflower oleosomes has a synergistic effect in improving the repair effect.

[0053] UV resistance test of the composition:

[0054] Ultraviolet rays are a type of high-energy radiation with high energy. They can destroy the keratin in the hair, such as breaking peptide bonds and disulfide bonds, causing the hair to lose elasticity and toughness and become easy to break. Randomly select several healthy hair bundles, apply equal amounts of samples of Comparative Examples 1-4 or Examples 1-2 in Table 4 to each of them, and then dry them under constant temperature and humidity conditions. The dried hair bundles are placed in a xenon lamp simulation box to simulate sunlight exposure for 24 hours. After the simulated exposure, they are taken out and tested for hair protein content. Accurately measure a certain amount of hair and a certain amount of deionized water, soak the hair in deionized water for 8 hours, and then use the Coomassie Brilliant Blue method to determine the protein content in the extract. The higher the protein content, the better the sample's resistance to UV damage. The test results of hair protein content are as follows: Figure 4 shown.

[0055] Figure 4 The test results of hair protein content are as follows: Figure 4 It can be seen that pomegranate seed oil and tomato fruit oil can provide a certain protein protection effect when used alone. When pomegranate seed oil, tomato fruit oil and safflower oil are compounded, the protein protection effect is further enhanced, indicating that the combination of the three can synergistically enhance the anti-UV effect and can effectively reduce the problem of hair protein loss caused by ultraviolet radiation.

[0056] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the spirit and scope of the technical solutions of the present invention, which should all be included in the scope of the claims of the present invention.

Claims

1. A plant nourishing and repairing composition, characterized in that: The plant nourishing and repairing composition consists of pomegranate seed oil, tomato fruit oil and safflower oleosomes, and the mass ratio of the pomegranate seed oil, tomato fruit oil and safflower oleosomes is 1:(0.25-3):(1-4).

2. The plant nourishing and repairing composition according to claim 1, characterized in that: The mass ratio of pomegranate seed oil, tomato fruit oil and safflower oleosomes is 1:(0.25-2):(2-3).

3. The plant nourishing and repairing composition according to claim 1 or 2, characterized in that: The preparation method of the plant nourishing and repairing composition comprises the following steps: firstly mixing pomegranate seed oil and tomato fruit oil evenly, and then adding safflower oleosomes and mixing evenly; or firstly mixing pomegranate seed oil and safflower oleosomes evenly, and then adding tomato fruit oil and mixing evenly.

4. Use of the plant nourishing and repairing composition according to claim 1 in preparing cosmetics with hair repairing effect.

5. The use according to claim 4, characterized in that: The cosmetics include shampoo products, hair care products, and scalp care products.

6. Use of the plant nourishing and repairing composition according to claim 1 in preparing cosmetics for improving hair combing properties.

7. Use of the plant nourishing and repairing composition according to claim 1 in the preparation of antioxidant functional cosmetics.

8. Use of the plant nourishing and repairing composition according to claim 1 in the preparation of anti-ultraviolet functional cosmetics.

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