Whitening and freckle removing formula as well as preparation process and application thereof

Abstract

The invention discloses a whitening and freckle-removing formula as well as a preparation method and application thereof, and belongs to the technical field of traditional Chinese medicines. The formula comprises the following components in percentage by mass: 1.200% of asiaticoside, 0.008%-0.16% of a radix paeoniae alba extract, 0.064%-0.128% of a radix salviae miltiorrhizae extract and 0.016%-0.032% of a honeysuckle flower extract. The asiaticoside, the radix salviae miltiorrhizae extract, the radix paeoniae alba extract and the honeysuckle flower extract are used as traditional Chinese medicine addition components, the proper addition amount of carbomer 940, glycerol and sodium hyaluronate is obtained by utilizing a single-factor experiment, and the matrix proportion of the mask is determined by combining an orthogonal experiment; the addition amount of the traditional Chinese medicine components is determined according to the tyrosinase activity inhibition ratio, and the mask formula is optimized. The'Qing, grass, silver and snow 'mask has good whitening and freckle removing effects, the tyrosinase activity inhibition rate is at least 85.12%, the DPPH free radical scavenging rate is at least 89.15%, and the moisturizing rate is at least 47.93%.

Description

Technical Field

[0001] The invention relates to a whitening and freckle-removing formula and a preparation method and application thereof, in particular to a "Qinghe Yinxue" whitening and freckle-removing formula and a preparation process thereof and application in a facial mask, belonging to the technical field of traditional Chinese medicines. Background Art

[0002] Facial masks are one of the most commonly used cosmetics in daily life. They are widely loved by consumers because of their convenience and good efficacy. In particular, the market demand for facial masks with whitening and freckle removal effects has remained high. With the improvement of the quality of life, people pay more and more attention to skin care and have higher requirements for skin care products. The facial masks with whitening and freckle removal effects on the market mainly come from some chemical agents with whitening and freckle removal effects. Although chemical agents have a certain effect on whitening and freckle removal, they are low in safety, highly irritating, and even potentially harmful to the human body. In comparison, traditional Chinese medicine whitening has the advantages of a long history, high efficiency, and safety. Combining ancient books and using modern science and technology, a new formula of Chinese herbal mask with high efficiency and safety for whitening and freckle removal is developed to meet market demand and has distinct advantages and great development prospects in the field of cosmetics research and development.

[0003] The present invention selects extracts of Centella asiatica, white peony root, salvia miltiorrhiza and honeysuckle as traditional Chinese medicine ingredients, and develops a traditional Chinese medicine mask with certain whitening, freckle removal and moisturizing effects on the basis of traditional mask ingredients, and conducts an efficacy evaluation on the mask. Summary of the invention

[0004] The purpose of the present invention is to provide a whitening and freckle-removing formula to solve the problems in the prior art, and the formula has excellent whitening and freckle-removing effects.

[0005] Another object of the present invention is to provide a preparation process of a whitening and freckle-removing formula, and the white peony root extract obtained by the process has a more excellent whitening and freckle-removing effect.

[0006] Another object of the present invention is to provide a whitening and freckle-removing formula for use in a facial mask.

[0007] Another object of the present invention is to provide a whitening and freckle-removing facial mask, which has a tyrosinase activity inhibition rate of at least 85.12%, a DPPH free radical scavenging rate of at least 89.15%, and a moisturizing rate of at least 47.93%.

[0008] Another object of the present invention is to provide a preparation process of a whitening and freckle-removing facial mask, wherein the facial mask obtained by the process has stable properties, a pH value of 6.37 that meets the standard, and is non-irritating.

[0009] In order to solve the above technical problems, the technical solution adopted by the present invention is:

[0010] A whitening and freckle-removing formula comprises the following components in percentage by mass: 1.200% of asiatica glycoside, 0.008%-0.16% of white peony root extract, 0.064%-0.128% of salvia miltiorrhiza extract and 0.016%-0.032% of honeysuckle extract.

[0011] A preparation process of a whitening and freckle-removing formula comprises the following steps:

[0012] S01, taking dried root and dried flower of white peony root in a mass ratio of (2-4):1, crushing and sieving, placing in a round-bottom flask, adding 12-15 times amount of water, heating and boiling for at least 3 times, each time for 1.5-2 hours; filtering, combining the filtrate, concentrating the filtrate, and making up the volume with distilled water to 1-2 times the total weight of the dried root and dried flower of white peony root, letting it stand, and taking the supernatant;

[0013] S02, adding granular activated carbon equivalent to 3-5% of the weight of the supernatant to the supernatant, stirring and adsorbing at 50-70°C, 200-500r / min for 1-2h, then standing at room temperature, and then standing at 0-4°C overnight, taking the supernatant, concentrating and drying to obtain white peony root extract;

[0014] S03, take 1.200% of Centella asiatica glycoside, 0.008%-0.16% of white peony extract, 0.064%-0.128% of Salvia miltiorrhiza extract and 0.016%-0.032% of honeysuckle extract, and mix them to obtain a whitening and freckle-removing formula.

[0015] In S01, the sieving is through a 50-100 mesh sieve.

[0016] In S02, the particle size of the granular activated carbon is greater than 0.175 mm.

[0017] The invention discloses an application of a whitening and freckle-removing formula in a facial mask, wherein the facial mask is a whitening and freckle-removing facial mask.

[0018] A whitening and freckle removing facial mask, the formula of which comprises the following components in percentage by mass: 1.2%-1.8% of carbomer 940, 4%-8% of glycerol, 0.100%-0.200% of sodium alginate, 0.6%-1% of sodium hyaluronate, 4%-5% of arbutin, 4%-5% of niacinamide, 3%-4% of D-sorbitol, 1%-2% of panthenol, 1%-2% of betaine, 0.500%-1.500% of 1,2-hexanediol, p-Hydroxyacetophenone 0.500%-1.500%, citric acid 0.120%-0.150%, triethanolamine 0.120%-0.150%, asiatica glycoside 1.200%, white peony root extract 0.008%-0.16%, salvia miltiorrhiza extract 0.064%-0.128%, honeysuckle extract 0.016%-0.032%, tea tree essential oil 0.300%-0.500% and the balance purified water.

[0019] A preparation process of a whitening and freckle-removing facial mask comprises the following steps:

[0020] Step 1: Prepare a sample with a facial mask liquid content of 30 g per portion, first prepare a 5% carbomer solution in advance, and allow it to swell fully to obtain a carbomer solution;

[0021] Step 2: dissolve appropriate amounts of Centella asiatica, Salvia miltiorrhiza extract, White Peony Root extract and Honeysuckle Flos extract in 10 mL of water, heat in a 50-60° C. water bath to dissolve, add the pre-prepared carbomer solution, glycerol and sodium alginate and stir evenly, which is recorded as Solution A;

[0022] Step 3, then add arbutin, D-sorbitol, panthenol, niacinamide, and p-hydroxyacetophenone into 10 mL of water, heat in a water bath at 50-60° C., and stir with a homogenizer to completely dissolve, which is recorded as Solution B;

[0023] Step 4, add sodium hyaluronate to 5 mL of water, heat in a 50-60° C. water bath with stirring until dissolved, and dropwise add 1,2-hexanediol and triethanolamine, which is referred to as Solution C;

[0024] Step 5: Add liquid C and liquid B to liquid A, then add panthenol, betaine and citric acid, heat in a water bath at 50-70°C, stir evenly again with a homogenizer, and then put into an ultrasonic instrument for defoaming, observe the clarity of the solution to determine whether the substance is completely dissolved and mixed evenly. If the solution is turbid, stir again with a homogenizer and ultrasonically defoam until the mask liquid is clear and free of impurities. After the prepared mask liquid cools down, add tea tree essential oil for fragrance and homogenize for 5-10 minutes.

[0025] Step six, soak the mask cloth in the mask liquid and package it.

[0026] The preparation method of white peony root and white peony flower in a mass ratio of (2-4):1 are taken, crushed and sieved, placed in a round-bottom flask, and 12-15 times the amount of water are added for heating and decoction for at least 3 times, each time for 1.5-2 hours; filtered, combined the filtrate, concentrated the filtrate, and then made up to 1-2 times the total weight of the white peony root and the white peony flower with distilled water, allowed to stand, and supernatant liquid was taken; granular activated carbon equivalent to 3-5% of the weight of the supernatant liquid was added to the supernatant liquid, stirred and adsorbed at 50-70°C, 200-500r / min for 1-2 hours, and then allowed to stand at room temperature, and then allowed to stand at 0-4°C overnight, and the supernatant liquid was taken, and concentrated and dried to obtain the white peony root and white peony flower extract.

[0027] In step 5, the stirring speed of the homogenizer is 100-200 r / min.

[0028] A facial mask obtained by a preparation process for a whitening and freckle-removing facial mask has a tyrosinase activity inhibition rate of at least 85.12%, a DPPH free radical scavenging rate of at least 89.15%, and a moisturizing rate of at least 47.93%.

[0029] The present invention has the following beneficial effects:

[0030] The present invention is about the preparation and efficacy evaluation of the "Qinghe Yinxue" whitening and freckle-removing facial mask. Centella asiatica, salvia miltiorrhiza extract, white peony extract and honeysuckle extract are used as Chinese medicine additives, and the appropriate addition amounts of carbomer 940, glycerin and sodium hyaluronate are obtained by single factor experiment, and the mask matrix ratio is determined by orthogonal experiment; and the addition amount of Chinese medicine ingredients is determined according to the inhibition rate of tyrosinase activity to optimize the mask formula. Results: Through orthogonal experiment, it was found that carbomer 940 was 1.8%, glycerol was 6.0%, and sodium hyaluronate was 1.0%; through the determination of tyrosinase inhibition rate, it was found that asiaticoside was 1.200%, white peony root was 0.160%, salvia miltiorrhiza was 0.064%, and honeysuckle was 0.032%; the added amounts of other ingredients were: sodium alginate (0.100%), arbutin (4.000%), niacinamide (4.000%), sorbitol (3.000%), panthenol (1.000%), betaine (1.000%), 1,2-hexanediol (0.500%), p-hydroxyacetophenone (0.500%), citric acid (0.120%), triethanolamine (0.120%), and purified water (75.404%). The "Qinghe Yinxue" mask has stable properties, a pH value of 6.37 that meets the standard, is non-irritating, has a DPPH free radical scavenging rate of 89.15%, has anti-aging ability, and a moisturizing rate of 47.93%. Conclusion: The "Qinghe Yinxue" mask has a good whitening and freckle removal effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 This is a comparison chart of tyrosinase inhibition rates;

[0032] Figure 2 This is a comparison chart of mice used in skin irritation experiments. DETAILED DESCRIPTION

[0033] The present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments. The following embodiments are only used to illustrate the present invention and are not intended to limit the scope of the present invention.

[0034] Example 1

[0035] 1. Reagents and Instruments

[0036] Glycerin (Xilong Scientific Co., Ltd., batch number: B2302242); Carbomer 940 (Domezi Biotechnology Co., Ltd., batch number: 20230220); Sodium alginate (Sinopharm Chemical Reagent Co., Ltd., batch number: 2020819); Citric acid (Sinopharm Chemical Reagent Co., Ltd., batch number: 20220929); Triethanolamine (Sinopharm Chemical Reagent Co., Ltd., batch number: 20220617); D-sorbitol (Sinopharm Chemical Reagent Co., Ltd., batch number: 20220901); Sodium hyaluronate (Guanying Biotechnology Co., Ltd., batch number: 20231019); Arbutin (Guanying Biotechnology Co., Ltd., batch number: 20231103); Niacinamide (Xi'an Yunhe Biotechnology Co., Ltd., batch number: YHSW211207-1); Betaine (Xi'an Zelang Biotechnology Co., Ltd., batch number: ZLSW211118-1), panthenol (Xi'an Zelang Biotechnology Co., Ltd., batch number: ZLSW211223-3); Centella asiatica glycoside (Shaanxi Angxi Biotechnology Co., Ltd., batch number: 20240128); white peony root extract (Xi'an Chenguang Biotechnology Co., Ltd., batch number: 20240112), Salvia miltiorrhiza extract (Xi'an Chenguang Biotechnology Co., Ltd., batch number: 20240108); honeysuckle extract (purchased from Shaanxi Zhiqi Biotechnology Co., Ltd., batch number: 221122-7); 1,2-hexanediol (Qingdao Yousu Chemical Technology Co., Ltd.); DPPH solution (Feijing Biotechnology Co., Ltd.); L-dopa (Guier Biotechnology Co., Ltd., batch number: 20240210), tyrosinase, PBS buffer (Guier Biotechnology Co., Ltd.).

[0037] ME104E electronic analytical balance (Mettler-Toledo Instrument Co., Ltd.), FSH-2A adjustable high-speed homogenizer (Shanghai Jinyi Instrument Technology Co., Ltd.), electric constant temperature blast drying oven DHG-9140A (Shanghai Jinghong Experimental Equipment Co., Ltd.), electronic balance (Tianjin Tianma Hengji Instrument Co., Ltd.), HH-S2 digital display constant temperature water bath (Jiangsu Jinyi Instrument Technology Co., Ltd.), KH-250B ultrasonic cleaner (Kunshan Hechuang Ultrasonic Instrument Co., Ltd.), ELISA reader (Tecan Austria GmbH), PHS-25 pH meter (Shanghai Yidian Scientific Instrument Co., Ltd.), high-speed desktop centrifuge TGL-16C (Shanghai Anting Scientific Instrument Factory), UV-visible spectrophotometer (Beijing Puxi General Instrument Co., Ltd.), and smart skin detector (Hefei Huya Technology Co., Ltd.).

[0038] 2. Experiments and Results

[0039] 2.1 Mask formula and preparation process

[0040] 2.1.1 Basic formula

[0041] The patch mask is mainly composed of mask liquid and mask cloth. The composition of the mask liquid includes solvent, moisturizer, emollient, functional active ingredients, rheology regulator, preservative, flavor, etc. Combined with the preliminary experiment, Carbomer 940 was preliminarily selected as the gel matrix of the mask; glycerin, D-sorbitol, sodium hyaluronate, and betaine were used as moisturizers; arbutin, niacinamide, Centella asiatica glycoside, Salvia miltiorrhiza extract, White Peony extract, and Honeysuckle extract were used as the main functional ingredients. The primary formula of the "Qinghe Yinxue" whitening and anti-freckle mask is shown in Table 1.

[0042] A preparation process of a whitening and freckle-removing formula, the preparation method of white peony root extract comprises the following steps:

[0043] S01, take dried root and dried flower of white peony root in a mass ratio of 3:1, crush them and pass through a 50-mesh sieve, place them in a round-bottom flask, add 12 times of water, heat and boil for 3 times, each time for 1.5 hours; filter, combine the filtrate, concentrate the filtrate, dilute with distilled water to 1 times the total weight of the dried root and dried flower of white peony root, let it stand, and take the supernatant;

[0044] S02, add granular activated carbon (particle size greater than 0.175mm) equivalent to 4% of the weight of the supernatant to the supernatant, stir and adsorb at 50°C, 200r / min for 1h, then let it stand at room temperature and then at 0°C overnight, take the supernatant, concentrate and dry to obtain the white peony extract.

[0045] Table 1 Primary formula of facial mask

[0046]

[0047]

[0048] 2.1.2 Preparation process

[0049] Prepare the sample with a mask liquid content of 30g per portion. First, prepare a 5% carbomer solution in advance to fully swell it, dissolve an appropriate amount of Centella asiatica, Salvia miltiorrhiza extract, White Peony extract and Honeysuckle extract in 10mL of water and heat in a 60°C water bath to dissolve, add the prepared carbomer solution, glycerol and sodium alginate and stir evenly, record as A liquid. Secondly, add arbutin, D-sorbitol, panthenol, niacinamide and p-hydroxyacetophenone to 10mL of water, heat in a 50°C water bath and stir with a homogenizer to completely dissolve it, record as B liquid. Add sodium hyaluronate to 5mL of water, heat in a 50°C water bath and stir until dissolved, add 1,2-hexanediol and triethanolamine dropwise, record as C liquid.

[0050] Add liquid C and liquid B to liquid A, and then add panthenol, betaine and citric acid. Heat in a 50°C water bath, stir again with a homogenizer, and put into an ultrasonic instrument for defoaming to observe the clarity of the solution to determine whether the substances are completely dissolved and mixed evenly. If the solution is turbid, stir again with a homogenizer and defoam ultrasonically until the mask liquid is clear and free of impurities. After the prepared mask liquid cools down, add tea tree essence to add fragrance, and homogenize for 5 minutes.

[0051] 2.2 Experimental study on facial mask formula

[0052] 2.2.1 Single-factor experimental design

[0053] 2.2.1.1 Selection of evaluation indicators

[0054] According to the requirements of the finished mask products, the most suitable addition amounts of Carbomer 940, glycerin and sodium hyaluronate were determined by single-factor experiments. Viscosity, uniformity and solubility were used as scoring criteria to select the appropriate research scope. The specific evaluation criteria are shown in Table 2.

[0055] Table 2 Evaluation criteria for single factor evaluation

[0056]

[0057] 2.2.1.2 Study on the amount of Carbomer 940 added to facial mask liquid

[0058] Under the condition that the addition amount of glycerol is 2% and the addition amount of sodium hyaluronate is 0.2% unchanged, Carbomer 940 is added in amounts of 0.8%, 1.2%, 1.5%, 1.8% and 2%, respectively, and mixed with 25 mL of water to prepare a mask matrix.

[0059] When the content of Carbomer 940 is less than 1%, the mask liquid becomes fluid with poor adhesion and is not easy to fit on the face; when the content is 2%, the mask liquid becomes a gel-like solid with poor ductility, which is not suitable for making a patch mask and has a thick and heavy texture that is not easily absorbed. After adding other ingredients, it is not easily dissolved and becomes flocculated, making the mask liquid turbid and having a poor sensory experience. Combined with the above evaluation criteria, the orthogonal experiment investigation level was selected.

[0060] 2.2.1.3 Study on the amount of glycerol added in facial mask liquid

[0061] Under the condition that the addition amount of Carbomer 940 is 0.8% and the addition amount of sodium hyaluronate is 0.2% unchanged, glycerin is added in amounts of 2.0%, 4.0%, 6.0%, 8.0% and 10.0%, respectively, and mixed with 25 mL of water to prepare a mask matrix.

[0062] When more glycerin is added, the product will be oily, thick and heavy when applied on the skin, and will not be easily absorbed; when less glycerin is added, the moisturizing effect is not obvious and does not meet the requirements of a facial mask. Combined with the above evaluation criteria, the orthogonal experiment investigation level was selected.

[0063] 2.2.1.4 Study on the amount of sodium hyaluronate added to facial mask liquid

[0064] Under the condition that the addition amount of glycerol is 2% and the addition amount of carbomer 940 is 0.80%, sodium hyaluronate is added in amounts of 0.2%, 0.4%, 0.6%, 0.8% and 1.0%, respectively, and mixed with 25 mL of water to prepare a mask matrix.

[0065] When the sodium hyaluronate content is greater than 1%, the sodium hyaluronate solubility is poor, and there are a large number of lumps in the mask liquid, which is turbid and too viscous. Combined with the above evaluation criteria, the orthogonal experimental investigation level is selected.

[0066] 2.2.2 Orthogonal experiment to optimize mask formula

[0067] According to the single factor experiment, the appropriate amount of Carbomer 940 added is 1.2%-1.8%. If the content is too low, the mask liquid texture will become fluid, and if the content is too high, it is not suitable for making a patch mask; the amount of glycerol added should be controlled at 4.0%-8.0%. When the content is high, the skin feel is poor, and if the content is too low, the effect is not obvious; the amount of sodium hyaluronate added is 0.6%-1.0%. When the content is high, the solubility is poor and affects the texture of the mask, and if the content is too low, the effect is not obvious. Based on this, L9 was conducted. (33) The orthogonal experiment was used to optimize the mask liquid formula. The liquid carrying rate was used as the evaluation standard for the preparation of the mask liquid matrix. The points were assigned according to Table 3. The specific orthogonal experimental design ratio is shown in Table 4.

[0068] Liquid carrying rate experiment: 1 Put the mask base cloth into a clean beaker, add 25g of mask sample, and after it is fully soaked, take out the mask cloth, hang it in the air for 2 minutes, and then record the remaining mask sample mass M in the beaker. 2 , the mass of the mask sample on the mask base fabric is M 3 =25-M 2 .

[0069] Liquid rate = M 3 / M 1 ×100%

[0070] Table 3 Liquid Carrying Rate Scoring Criteria

[0071]

[0072] Note: It is calculated that the liquid carrying rate of commercially available facial masks is 1550%, and this is used as the standard for scoring.

[0073] Table 4 Orthogonal experimental factors and levels

[0074]

[0075] According to the data in Table 5, the influence of the three factors on the mask liquid matrix from high to low are carbomer 940, glycerin, and sodium hyaluronate, and the optimal levels of the three factors are A3, B2, and C3, respectively. Therefore, the final selection of carbomer 940 added at 1.8%, glycerin added at 6.0%, and sodium hyaluronate added at 1% was the optimal combination for preparing the mask liquid matrix.

[0076] Table 5 Orthogonal experiment results

[0077]

[0078]

[0079] 2.2.3 Amount of main Chinese herbal ingredients added to facial masks

[0080] Through single-factor experiments and orthogonal experiments, the amount of ingredients other than Chinese medicine ingredients to be added was preliminarily determined. Since the addition of Chinese medicine does not affect the formation of the mask, but only affects the efficacy of the mask, and the main effect of the "Qinghe Yinxue" mask is whitening and removing spots, melanin is the main factor affecting skin color and skin scars, and tyrosinase is the key enzyme in the melanin synthesis pathway, so the optimal formula of Chinese medicine effective ingredients was selected through experiments on tyrosinase inhibition rate.

[0081] Since other Chinese herbal ingredients have colors, when too much Chinese herbal ingredients are added, the color of the mask liquid will change greatly, affecting the appearance of the product. Therefore, the amount of Chinese herbal extract added in a 30g mask liquid is preliminarily determined through sensory evaluation: 20mg or 40mg of white peony extract, 16mg or 32mg of salvia miltiorrhiza extract, and 4mg or 8mg of honeysuckle extract. However, asiatica glycoside has no effect on the color and molding of the mask liquid. The maximum solubility of asiatica glycoside in the mask liquid is 15mg / mL. Therefore, the amount of asiatica glycoside added in a 30g mask liquid is determined to be 300mg. Mask liquid samples were prepared according to the amount of Chinese herbal extract added in Table 6 for experiments.

[0082] Table 6 Addition amount of Chinese herbal medicine extract

[0083]

[0084] Determination method: The tyrosinase inhibition rate of the sample was determined by the DOPA rate oxidation method. Phosphate buffer (PBS), 1 mg / mL L-DOPA and mask solution were precisely added to the 96-well plate according to Table 7 using a pipette. After being placed in a 37°C shaking incubator for 10 minutes, 0.1 mg / ml tyrosinase solution was added. After being protected from light for 10 minutes, the sample was placed in an ELISA reader, and the absorbance was measured at 475 nm, and the inhibition rate of the sample on tyrosinase was calculated. Each group was measured three times in parallel, and the average value was taken.

[0085]

[0086] Where, OD 1 is the absorbance value of the experimental group; OD 2 is the absorbance value of the experimental control group; OD 3 is the absorbance value of the blank group; OD 4 is the absorbance value of the blank control group.

[0087] Table 7 Reaction solution composition

[0088]

[0089] In Table 7, group 1 is the experimental group, group 2 is the experimental control group, group 3 is the blank group, and group 4 is the blank control group.

[0090] Table 8 Tyrosinase inhibition rate experimental results

[0091]

[0092] like Figure 1 As shown in the figure, the optimal formula of adding Chinese herbal medicine ingredients was obtained by comparing single variables. When white peony extract was used as a variable, group A had a high inhibition rate when compared with group B, and the amount of white peony added was 40 mg; when salvia miltiorrhiza extract was used as a variable, group A had a high inhibition rate when compared with group C, and the amount of salvia miltiorrhiza added was 16 mg; when honeysuckle extract was used as a variable, group B had a high inhibition rate when compared with group D, and the amount of honeysuckle added was 8 mg. Therefore, the amount of Chinese herbal medicine ingredients added was: white peony: 40 mg, salvia miltiorrhiza 16 mg, honeysuckle 8 mg, and asiaticoside 300 mg.

[0093] 3. Product efficacy and performance evaluation

[0094] 3.1 Stability

[0095] 3.1.1 Centrifugation experiment

[0096] At room temperature, take 3 portions of 5g of mask liquid in parallel, put them into 10mL centrifuge tubes, centrifuge at 2500r / min for 10min and 30min respectively, and then take them out to observe whether the mask samples have water precipitation and stratification. Results: The mask samples did not have water precipitation and stratification at 10min and 30min, indicating that the mask has good stability.

[0097] 3.1.2 Cold and heat resistance test

[0098] Three mask samples were placed in 10ml centrifuge tubes, sealed with sealing film, and kept at high temperature (40±1)℃, low temperature (-8±2)℃ and room temperature for 24 hours. After 24 hours, the high temperature group and the low temperature group were taken out and compared with the room temperature group after returning to room temperature to observe whether there were significant differences. Results: The mask samples returned to room temperature were compared with the room temperature group. It was observed that there was no change in shape, appearance and color in the three groups, which shows that the mask has good cold and heat resistance.

[0099] 3.2 Determination of pH value

[0100] According to GB / T 13531.1-2008 "General Test Methods for Cosmetics", the pH value of the mask is measured, and the pH range is required to be 3.5-8.5. Test method: Weigh 1.0g of sample, add 20mL of laboratory water that has been boiled and cooled, heat to 40±1℃ and stir continuously, cool to room temperature and measure with a calibrated pH meter, and measure 3 groups in parallel to take the average value. The pH of the mask was measured to be 6.37, which meets the requirements.

[0101] 3.3 Moisture retention test

[0102] Take 9 medical tapes of equal size, place the medical tapes in a culture dish, evenly spread the sample on the surface of the medical tape to simulate the skin absorption state, measure the initial weight M0, place it in a 38°C oven for 1 hour, and measure the weight M1 after 1 hour (the weight of the culture dish is Mb).

[0103] Moisture retention rate (%) = (M1-Mb) / (M0-Mb) × 100%

[0104] After testing, the moisturizing rate of the mask is 47.93%, and the mask has good moisturizing properties.

[0105] 3.4 Determination of DPPH free radical scavenging ability

[0106] Add 1mL of the test solution and 1mL of DPPH reagent to the test tube, shake well and place in the dark for 30 minutes, measure the absorbance of each reaction solution at 517nm, and calculate the scavenging rate of each sample to be tested for DPPH free radicals. The calculation shows that the scavenging rate of the mask for DPPH free radicals reaches 89.15%, which has a certain anti-aging ability.

[0107] DPPH free radical scavenging rate = [1-(A1-A2) / A3] × 100%

[0108] (A1 is the absorbance of the mixture of each sample to be tested and DPPH reagent; A2 is the absorbance of the mixture of the sample to be tested and anhydrous ethanol; A3 is the absorbance of the mixture of DPPH reagent and distilled water).

[0109] 3.5 Skin irritation test

[0110] like Figure 2 As shown, 6 mice were taken, each with two mice in a group, and divided into a blank group, an experimental group, and a control group. After random grouping, all mice were depilated on the back, and then the skin was scratched. The skin on one side was not scratched, and the skin on the other side was scratched in the shape of a "-" to make the skin surface bleed slightly. The blank control group was coated with pure water, the experimental group was coated with a mask liquid, and the control group was coated with a commercially available mask (L'Oreal Revitalizing Hyaluronic Acid Mask). The mask liquid was applied at intervals of 30 minutes, 40 minutes, 60 minutes, 90 minutes, and 120 minutes, respectively, for a total of 5 times. Observe the skin reactions of the applied parts on both sides of the mice at 1h, 24h, 48h, 72h, and 96h after application to see whether erythema, edema, and ulceration occur, and determine the intensity of skin irritation.

[0111] After the experiment, it was found that all mice had no systemic poisoning and no death. The behavior, activity, excretion, diet and appearance of mice in each group were normal. No erythema, edema and ulceration were found on the intact skin and damaged skin of mice in the blank control group and the experimental group at 1h, 24h, 48h and 72h after applying the mask liquid. This means that the mask liquid is non-irritating to the skin and can be used safely.

[0112] 3.6 Population Trial Experiment

[0113] 3.6.1 Analysis of the immediate effects of facial masks

[0114] Take 30g of the prepared mask liquid, soak the mask cloth completely, and apply it on the face of the tester for 30 minutes. Use a skin tester to test the changes in various skin indicators before and after use at a fixed position on the face. From Table 9, it can be seen that the mask can instantly control oil, has obvious skin rejuvenation effects, and has good hydration and moisturizing effects.

[0115] Table 9 Immediate effect evaluation table

[0116]

[0117] 3.6.2 Trial Evaluation

[0118] 10 volunteers were selected to use the mask continuously for 3 days and then scored the mask according to the standards in Table 10. The evaluation indicators included mildness, comfort and absorbency for the final evaluation of the mask.

[0119] Table 10 Population trial scoring criteria

[0120]

[0121] After the trial, the volunteers scored above 4 points, indicating that the mask is gentle and comfortable to use without allergies, stinging or redness, has good absorbency, and fits well to the face. After use, the skin feels refreshed and non-sticky. After continuous use for a period of time, the skin is hydrated and delicate, indicating that the mask is effective.

[0122] 4. Conclusion

[0123] The optimal formula of the "Qinghe Yinxue" mask was finally determined through single-factor experiments, orthogonal experiments and the inhibition rate of tyrosinase, as shown in Table 11. The "Qinghe Yinxue" mask has a uniform texture, appropriate viscosity, and a liquid rate of more than 1000%. It can effectively inhibit the production of melanin, and is cold-resistant and heat-resistant, centrifugal and non-stratified. The pH is 6.37, which meets the standard, the moisturizing rate is 47.93%, and the DPPH free radical scavenging rate is 89.15%. It has a certain anti-aging ability, is safe and non-irritating, and is comfortable to use. It is a whitening and anti-freckle mask with good efficacy and performance.

[0124] Table 11 Optimal formula of “Qinghe Yinxue” facial mask

[0125]

[0126] Cosmetics usually achieve the effect of whitening and removing freckles by inhibiting the production of melanin. However, it is currently believed that melanin is produced under the action of tyrosinase, which is a key enzyme in the melanin synthesis pathway. Therefore, most whitening and freckle removal products are tyrosinase inhibitors. Centella asiatica can promote fibroblast proliferation and collagen synthesis, and has a good anti-acne and anti-inflammatory effect; Danshensu and protocatechuic aldehyde in Danshen extract can inhibit the activity of tyrosinase and play a whitening effect. Among them, Tanshinone ⅡA has a significant effect on treating acne and inhibiting scars; there are as many as 6 compounds in white peony extract that can inhibit tyrosinase. This shows that traditional Chinese medicine is widely used in whitening and freckle removal cosmetics.

[0127] This embodiment determines the amount of Chinese medicine ingredients added by the experiment of tyrosinase inhibition rate, and combines sensory evaluation to obtain that when the content of Centella asiatica glycoside is 1.2% and the content of white peony extract is 0.16%, the inhibition rate of tyrosinase reaches 76.73%, which has a good resistance to the formation of melanin. Arbutin and nicotinamide can interfere with melanin production and treat melanin deposition in cosmetics to play a whitening and freckle removal effect. When the amount added is greater than 4%, after standing for 4 hours, it will saturate with other ingredients in the mask liquid to precipitate crystalline precipitation, making the mask liquid unstable. At the same time, the mask has a clearance rate of 89.15% for DPPH free radicals, indicating that the mask also has a certain anti-aging ability.

[0128] According to the orthogonal experiment, Carbomer 940 has the greatest impact on the formation of the mask. Carbomer 940 has good thickening, suspending and film-forming properties, which can improve the stability of the product, allowing the drug to stay on the skin surface for a long time, with good spreadability and no greasy feeling, promoting drug absorption, and no irritation, toxicity or allergic reaction to the skin. Therefore, Carbomer 940 was selected as a thickener and used in combination with glycerin and sodium hyaluronate to obtain a whitening and anti-freckle mask with stable properties and uniform and delicate texture.

[0129] This experiment combines ancient Chinese medicine classics with modern medical research to obtain a whitening and anti-freckle Chinese medicine mask with extracts of salvia miltiorrhiza, Centella asiatica, white peony root, and honeysuckle as the main ingredients, and uses orthogonal experiments and tyrosinase inhibition experiments to optimize the formula ratio. The whitening and anti-freckle Chinese medicine mask is cold-resistant and heat-resistant, has no stratification phenomenon during centrifugation, has a pH value of 6.37, a moisturizing rate of 47.93%, is non-irritating to the skin, and all indicators meet the QB / T2872-2017 standard. It has distinct advantages and great development prospects in the field of cosmetics research and development.

[0130] Example 2

[0131] The difference between this embodiment and embodiment 1 is only that:

[0132] A whitening and freckle-removing formula comprises the following components in percentage by mass: 1.200% of asiatica glycoside, 0.008% of white peony root extract, 0.128% of salvia miltiorrhiza extract and 0.016% of honeysuckle extract.

[0133] A preparation process of a whitening and freckle-removing formula comprises the following steps:

[0134] S01, take dried root and dried flower of white peony root in a mass ratio of 2:1, crush them and pass through a 100-mesh sieve, place them in a round-bottom flask, add 15 times the amount of water, heat and boil for 4 times, each time for 2 hours; filter, combine the filtrate, concentrate the filtrate, dilute it with distilled water to 2 times the total weight of the dried root and dried flower of white peony root, let it stand, and take the supernatant;

[0135] S02, adding granular activated carbon equivalent to 3% of the weight of the supernatant to the supernatant, stirring and adsorbing at 70°C, 500r / min for 2h, then standing at room temperature, and then standing at 4°C overnight, taking the supernatant, concentrating and drying to obtain white peony root extract;

[0136] S03, take 1.200% of Centella asiatica glycoside, 0.008% of white peony root extract, 0.128% of Salvia miltiorrhiza extract and 0.016% of honeysuckle extract, and mix them to obtain a whitening and freckle-removing formula.

[0137] The present embodiment discloses an application of a whitening and freckle-removing formula in a facial mask, and the facial mask is a whitening and freckle-removing facial mask.

[0138] A whitening and freckle-removing facial mask, the formula of which comprises the following components in percentage by mass: 1.2% of carbomer 940, 8% of glycerin, 0.200% of sodium alginate, 1% of sodium hyaluronate, 5% of arbutin, 5% of niacinamide, 4% of D-sorbitol, 2% of panthenol, 2% of betaine, 1.5% of 1,2-hexanediol, 1.5% of p-hydroxyacetophenone, 0.150% of citric acid, 0.150% of triethanolamine, 1.200% of asiatica glycoside, 0.008% of white peony root extract, 0.128% of salvia miltiorrhiza extract, 0.016% of honeysuckle extract, 0.300% of tea tree essential oil and the balance of purified water.

[0139] A preparation process of a whitening and freckle-removing facial mask comprises the following steps:

[0140] Step 1: Prepare a sample with a facial mask liquid content of 30 g per portion, first prepare a 5% carbomer solution in advance, and allow it to swell fully to obtain a carbomer solution;

[0141] Step 2, dissolve appropriate amounts of Centella asiatica glycoside, Salvia miltiorrhiza extract, White Peony Root extract and Honeysuckle Flos extract in 10 mL of water, heat in a 50° C. water bath to dissolve, add the pre-prepared carbomer solution, glycerol and sodium alginate and stir evenly, which is recorded as Solution A;

[0142] Step 3, then add arbutin, D-sorbitol, panthenol, niacinamide, and p-hydroxyacetophenone into 10 mL of water, heat in a 50° C. water bath, and stir with a homogenizer at 100 r / min to completely dissolve, which is recorded as Solution B;

[0143] Step 4, add sodium hyaluronate to 5 mL of water, heat in a 50°C water bath with stirring until dissolved, and dropwise add 1,2-hexanediol and triethanolamine, which is referred to as Solution C;

[0144] Step 5, add liquid C and liquid B to liquid A, then add panthenol, betaine and citric acid, heat in a 50°C water bath, stir again with a 100r / min homogenizer, and then put into an ultrasonic instrument for defoaming, observe the clarity of the solution to determine whether the substance is completely dissolved and mixed evenly, if the solution is turbid, stir again with a homogenizer and ultrasonic defoaming until the mask liquid is clear and free of impurities, add 0.300% tea tree essential oil to the prepared mask liquid after cooling, and homogenize for 10 minutes;

[0145] Step six, soak the mask cloth in the mask liquid and package it.

[0146] The facial mask obtained by the preparation process of a whitening and freckle-removing facial mask in this embodiment has a tyrosinase activity inhibition rate of 85.12%, a DPPH free radical scavenging rate of 89.79%, and a moisturizing rate of 49.21%.

[0147] Example 3

[0148] A whitening and freckle-removing formula comprises the following components in percentage by mass: 1.200% of asiatica glycoside, 0.14% of white peony root extract, 0.1% of salvia miltiorrhiza extract and 0.025% of honeysuckle extract.

[0149] A preparation process of a whitening and freckle-removing formula comprises the following steps:

[0150] S01, take dried root and dried flower of white peony root in a mass ratio of 4:1, crush them and pass through a 100-mesh sieve, place them in a round-bottom flask, add 14 times the amount of water, heat and boil for 3 times, each time for 1.8 hours; filter, combine the filtrate, concentrate the filtrate, dilute the volume with distilled water to 1.5 times the total weight of the dried root and dried flower of white peony root, let it stand, and take the supernatant;

[0151] S02, adding granular activated carbon equivalent to 5% of the weight of the supernatant to the supernatant, stirring and adsorbing at 70°C, 500r / min for 1.5h, then standing at room temperature, and then standing at 2°C overnight, taking the supernatant, concentrating and drying to obtain white peony root extract;

[0152] S03, take 1.200% of Centella asiatica glycoside, 0.14% of white peony root extract, 0.1% of Salvia miltiorrhiza extract and 0.025% of honeysuckle extract, and mix them to obtain a whitening and freckle-removing formula.

[0153] The invention discloses an application of a whitening and freckle-removing formula in a facial mask, wherein the facial mask is a whitening and freckle-removing facial mask.

[0154] A whitening and freckle-removing facial mask, the formula of which comprises the following components in percentage by mass: 1.6% carbomer 940, 6% glycerin, 0.100% sodium alginate, 0.8% sodium hyaluronate, 4.5% arbutin, 4.5% niacinamide, 3.5% D-sorbitol, 1.5% panthenol, 1.5% betaine, 1.0% 1,2-hexanediol, 1.0% p-hydroxyacetophenone, 0.130% citric acid, 0.130% triethanolamine, 1.200% asiatica glycoside, 0.14% white peony root extract, 0.1% salvia miltiorrhiza extract, 0.025% honeysuckle extract, 0.500% tea tree essential oil and the balance of purified water.

[0155] A preparation process of a whitening and freckle-removing facial mask comprises the following steps:

[0156] Step 1: Prepare a sample with a facial mask liquid content of 30 g per portion, first prepare a 5% carbomer solution in advance, and allow it to swell fully to obtain a carbomer solution;

[0157] Step 2: dissolve appropriate amounts of Centella asiatica glycoside, Salvia miltiorrhiza extract, White Peony Root extract and Honeysuckle Flos extract in 10 mL of water, heat in a 55° C. water bath to dissolve, add the pre-prepared carbomer solution, glycerol and sodium alginate and stir evenly, which is recorded as Solution A;

[0158] Step 3, then add arbutin, D-sorbitol, panthenol, niacinamide, and p-hydroxyacetophenone into 10 mL of water, heat in a 60° C. water bath, and stir with a homogenizer at 200 r / min to completely dissolve, which is recorded as Solution B;

[0159] Step 4, add sodium hyaluronate to 5 mL of water, heat in a 60°C water bath with stirring until dissolved, and dropwise add 1,2-hexanediol and triethanolamine, which is referred to as Solution C;

[0160] Step 5: Add liquid C and liquid B to liquid A, then add panthenol, betaine and citric acid, heat in a 70°C water bath, stir again with a 200r / min homogenizer, and then put into an ultrasonic instrument for defoaming, observe the clarity of the solution to determine whether the substance is completely dissolved and mixed evenly. If the solution is turbid, stir again with a homogenizer and ultrasonically defoam until the mask liquid is clear and free of impurities. After the prepared mask liquid cools down, add tea tree essential oil for fragrance and homogenize for 8 minutes;

[0161] Step six, soak the mask cloth in the mask liquid and package it.

[0162] The facial mask obtained by the preparation process of a whitening and freckle-removing facial mask in this embodiment has a tyrosinase activity inhibition rate of 89.45%, a DPPH free radical scavenging rate of 90.28%, and a moisturizing rate of 48.94%.

[0163] Comparative Example 1

[0164] The difference between this comparative example and Example 1 is only that:

[0165] The preparation process of white peony root extract uses only white peony root dried flowers as extraction raw materials.

[0166] Comparative Example 2

[0167] The difference between this comparative example and Example 1 is only that:

[0168] The preparation process of white peony root extract uses only the dried root of white peony root as the extraction raw material.

[0169] Comparative Example 3

[0170] The difference between this comparative example and Example 1 is only that:

[0171] The preparation process of white peony root extract is to use white peony root dried root and white peony flower dried flower in a mass ratio of 1:1 as extraction raw materials.

[0172] Comparative Example 4

[0173] The difference between this comparative example and Example 1 is only that:

[0174] The preparation process of white peony root extract is to use white peony root dried root and white peony flower dried flower in a mass ratio of 5:1 as extraction raw materials.

[0175] Comparative Example 5

[0176] The difference between this comparative example and Example 1 is only that:

[0177] The extraction process only uses S01. The supernatant obtained by S01 is allowed to stand at room temperature and then at 0°C overnight. The supernatant is taken and concentrated and dried to obtain the white peony root extract.

[0178] Comparative Example 6

[0179] The difference between this comparative example and Example 1 is that in S02, the supernatant is stirred and adsorbed only at room temperature, and is not stirred and adsorbed at high temperature.

[0180] Comparative Example 7

[0181] The only difference between this comparative example and Example 1 is that the formula uses commercially available white peony root extract, white peony root extract (Xi'an Chenguang Biotechnology Co., Ltd., batch number: 20240112).

[0182] The data of tyrosinase activity inhibition rate, DPPH free radical scavenging rate and moisturizing rate of the facial masks obtained using Comparative Examples 1 to 7 are shown in Table 12 below.

[0183] Table 12 Comparative Example Data Table

[0184]

[0185] It should be understood that in order to streamline the present disclosure and aid in understanding one or more of the various inventive aspects, in the above description of exemplary embodiments of the present invention, various features of the present invention are sometimes grouped together into a single embodiment, figure, or description thereof. However, this disclosed method should not be interpreted as reflecting the intention that the claimed invention requires more features than those expressly recited in each claim. Rather, as reflected in the claims, inventive aspects lie in less than all of the features of the previously disclosed embodiments. Therefore, the claims that follow the detailed description are hereby expressly incorporated into the detailed description, with each claim itself serving as a separate embodiment of the present invention.

[0186] Although the present invention has been described according to a limited number of embodiments, it will be apparent to those skilled in the art, with the benefit of the above description, that other embodiments may be envisioned within the scope of the invention thus described. In addition, it should be noted that the language used in this specification is selected primarily for readability and teaching purposes, rather than for explaining or defining the subject matter of the present invention. Therefore, many modifications and variations will be apparent to those skilled in the art without departing from the scope and spirit of the appended claims. The disclosure of the present invention is illustrative, not restrictive, with respect to the scope of the present invention, which is defined by the appended claims.

[0187] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A whitening and freckle-removing formula, characterized in that: The invention comprises the following components in percentage by mass: 1.200% of asiatica glycoside, 0.008%-0.16% of white peony root extract, 0.064%-0.128% of salvia miltiorrhiza root extract and 0.016%-0.032% of honeysuckle flower extract.

2. The preparation process of a whitening and freckle-removing formula according to claim 1, characterized in that: The following steps are involved: S01, taking dried root and dried flower of white peony root in a mass ratio of (2-4):1, crushing and sieving, placing in a round-bottom flask, adding 12-15 times amount of water, heating and boiling for at least 3 times, each time for 1.5-2 hours; filtering, combining the filtrate, concentrating the filtrate, and making up the volume with distilled water to 1-2 times the total weight of the dried root and dried flower of white peony root, letting it stand, and taking the supernatant; S02, adding granular activated carbon equivalent to 3-5% of the weight of the supernatant to the supernatant, stirring and adsorbing at 50-70°C, 200-500r / min for 1-2h, then standing at room temperature, and then standing at 0-4°C overnight, taking the supernatant, concentrating and drying to obtain white peony root extract; S03, take 1.200% of Centella asiatica glycoside, 0.008%-0.16% of white peony extract, 0.064%-0.128% of Salvia miltiorrhiza extract and 0.016%-0.032% of honeysuckle extract, and mix them to obtain a whitening and freckle-removing formula.

3. The preparation process of a whitening and freckle-removing formula according to claim 2, characterized in that: In S01, the sieving is through a 50-100 mesh sieve.

4. The preparation process of a whitening and freckle-removing formula according to claim 2, characterized in that: In S02, the particle size of the granular activated carbon is greater than 0.175 mm.

5. The use of a whitening and freckle-removing formula in a facial mask according to claim 1, characterized in that: The mask is a whitening and anti-freckle mask.

6. A whitening and freckle removing facial mask, characterized in that: The formula includes the following components in percentage by mass: carbomer 940 1.2%-1.8%, glycerin 4%-8%, sodium alginate 0.100%-0.200%, sodium hyaluronate 0.6%-1%, arbutin 4%-5%, niacinamide 4%-5%, D-sorbitol 3%-4%, panthenol 1%-2%, betaine 1%-2%, 1,2-hexanediol 0.500%-1.500%, p-hydroxyphenylethyl Ketone 0.500%-1.500%, citric acid 0.120%-0.150%, triethanolamine 0.120%-0.150%, asiatica glycoside 1.200%, white peony extract 0.008%-0.16%, salvia miltiorrhiza extract 0.064%-0.128%, honeysuckle extract 0.016%-0.032%, tea tree essential oil 0.300%-0.500% and the balance of purified water.

7. A preparation process of a whitening and freckle-removing facial mask, characterized in that: The following steps are involved: Step 1: Prepare a sample with a facial mask liquid content of 30 g per portion, first prepare a 5% carbomer solution in advance, and allow it to swell fully to obtain a carbomer solution; Step 2: dissolve appropriate amounts of Centella asiatica, Salvia miltiorrhiza extract, White Peony Root extract and Honeysuckle Flos extract in 10 mL of water, heat in a 50-60° C. water bath to dissolve, add the pre-prepared carbomer solution, glycerol and sodium alginate and stir evenly, which is recorded as Solution A; Step 3, then add arbutin, D-sorbitol, panthenol, niacinamide, and p-hydroxyacetophenone into 10 mL of water, heat in a water bath at 50-60° C., and stir with a homogenizer to completely dissolve, which is recorded as Solution B; Step 4, add sodium hyaluronate to 5 mL of water, heat in a 50-60° C. water bath with stirring until dissolved, and dropwise add 1,2-hexanediol and triethanolamine, which is referred to as Solution C; Step 5: Add liquid C and liquid B to liquid A, then add panthenol, betaine and citric acid, heat in a water bath at 50-70°C, stir evenly again with a homogenizer, and then put into an ultrasonic instrument for defoaming, observe the clarity of the solution to determine whether the substance is completely dissolved and mixed evenly. If the solution is turbid, stir again with a homogenizer and ultrasonically defoam until the mask liquid is clear and free of impurities. After the prepared mask liquid cools down, add tea tree essential oil for fragrance and homogenize for 5-10 minutes. Step six, soak the mask cloth in the mask liquid and package it.

8. The preparation process of a whitening and freckle-removing facial mask according to claim 7, characterized in that: The preparation method of white peony root and white peony flower in a mass ratio of (2-4):1 are taken, crushed and sieved, placed in a round-bottom flask, and 12-15 times the amount of water are added for heating and decoction for at least 3 times, each time for 1.5-2 hours; filtered, combined the filtrate, concentrated the filtrate, and then made up to 1-2 times the total weight of the white peony root and the white peony flower with distilled water, allowed to stand, and supernatant liquid was taken; granular activated carbon equivalent to 3-5% of the weight of the supernatant liquid was added to the supernatant liquid, stirred and adsorbed at 50-70°C, 200-500r / min for 1-2 hours, and then allowed to stand at room temperature, and then allowed to stand at 0-4°C overnight, and the supernatant liquid was taken, and concentrated and dried to obtain the white peony root and white peony flower extract.

9. The preparation process of a whitening and freckle-removing facial mask according to claim 7, characterized in that: In step 5, the stirring speed of the homogenizer is 100-200 r / min.

10. The facial mask obtained by the preparation process of a whitening and anti-freckle facial mask according to any one of claims 7 to 9, characterized in that: The mask had a tyrosinase activity inhibition rate of at least 85.12%, a DPPH free radical scavenging rate of at least 89.15%, and a moisturizing rate of at least 47.93%.

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