Paeonia suffruticosa-containing double-resistance composition as well as preparation method and application thereof

Abstract

The invention relates to the technical field of cosmetics, and aims to provide a preparation method of a peony-containing double-resistant composition, which utilizes a compound enzyme technology to decompose components such as cellulose, hemicelluloses and pectin in cell walls and intercellular substances of moutan barks and destroy cell wall structures through the synergistic effect of cellulase, hemicelluloses and pectinase so as to improve the anti-inflammatory effect of the peony barks. And paeonol in cells can be released more easily, so that the extraction efficiency is improved, and the extraction time is shortened.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and more specifically, to a double-antibody composition containing peony, and a preparation method and application thereof. Background Art

[0002] Paeonia tannin is a phenolic compound extracted and separated from the root bark of peony, which has strong antioxidant capacity. Phenolic compounds can remove free radicals by providing hydrogen atoms. Free radicals are one of the important factors leading to cellular oxidative damage and aging. When free radicals attack cells, paeonia tannin can react with them to prevent free radicals from damaging biological macromolecules (such as DNA, proteins and lipids) in the cells. In addition, peony extracts also contain flavonoids. Flavonoids also have antioxidant properties. They can chelate metal ions, reduce the free radicals catalyzed by metal ions, and can remove various active oxygen free radicals, such as superoxide anion free radicals, hydroxyl free radicals, etc. through their own redox reactions.

[0003] In terms of skin, peony ol can protect the collagen and elastic fibers of the skin. With the increase of age and the influence of external environmental factors (such as ultraviolet radiation), the collagen and elastic fibers in the skin will gradually be destroyed, leading to sagging skin and wrinkles. The antioxidant components in peony extract can prevent free radicals from degrading collagen and elastic fibers, maintaining the elasticity and firmness of the skin, thus playing an anti-aging role.

[0004] At present, the methods for extracting peony ol from peony root bark mainly include solvent extraction, ultrasound-assisted extraction and microwave-assisted extraction. Traditional solvent extraction methods, such as ethanol reflux extraction, often have the problem of low extraction efficiency. Although ultrasound-assisted extraction can improve the extraction efficiency to a certain extent, it may damage the structure of the active ingredients of the extract; high-intensity ultrasound may cause some heat-sensitive antioxidant components to degrade or change their structure, thereby reducing their antioxidant activity. Microwave-assisted extraction may have the problem of uneven heating, resulting in unstable extraction effect. Summary of the invention

[0005] In view of the shortcomings of the prior art, the purpose of the present invention is to provide a method for preparing a dual-antibody composition containing peony, which is used to solve the technical problems of long extraction time and low yield of the existing extraction method.

[0006] To achieve the above object, the present invention adopts the following technical solution:

[0007] A method for preparing a dual-antibody composition containing peony comprises the following steps:

[0008] (1) adding the crushed Paeonia suffruticosa root powder into a buffer solution to prepare a suspension;

[0009] (2) adding the composite enzyme preparation to the suspension, stirring thoroughly, and performing enzymolysis reaction at 40-50° C. for 1-3 hours to obtain an enzymolysis solution;

[0010] (3) adding ethanol to the enzymatic hydrolyzate, mixing well, extracting in a reflux device for 1-2 hours, then cooling to room temperature, removing the residue, and recovering the filtrate;

[0011] (4) subjecting the filtrate to vacuum distillation, collecting the distillate, crystallizing and filtering to obtain a crystal, and drying to obtain the product.

[0012] Preferably, in step (1), the buffer solution is an acetic acid-sodium acetate buffer solution, and its pH value is 4.5-5.5.

[0013] Preferably, in step (1), the solid-liquid ratio of Paeonia suffruticosa powder to buffer solution is 1 g:(10-20 ml).

[0014] Preferably, in step (2), the composite enzyme preparation consists of cellulase, hemicellulase and pectinase in a weight ratio of 3:1.5:1.

[0015] Preferably, in step (2), the added amount of the complex enzyme preparation is 2-5% of the weight of the peony bark powder.

[0016] Preferably, in step (3), the material-liquid ratio of the enzymatic hydrolysate to ethanol is 1 ml:(8-12 ml).

[0017] Preferably, in step (3), the concentration of ethanol is 60-80%.

[0018] Another object of the present invention is to provide a dual-antibody composition containing peony, which is prepared by the aforementioned preparation method.

[0019] Another object of the present invention is to provide an application of a dual-antibody composition containing peony, by adding the aforementioned dual-antibody composition containing peony to cosmetics to prepare cosmetics with antioxidant and anti-aging effects.

[0020] Preferably, the cosmetic is in the form of an aqueous solution, emulsion, paste, powder, oil or mask.

[0021] Compared with the prior art, the present invention has the following beneficial effects: the present invention utilizes composite enzyme technology to decompose cellulose, hemicellulose, pectin and other components in the cell wall and intercellular matrix of peony bark through the synergistic action of cellulase, hemicellulose and pectinase, destroys the cell wall structure, and makes it easier to release the peony phenol in the cell, thereby improving the extraction efficiency and shortening the extraction time. DETAILED DESCRIPTION

[0022] Example 1

[0023] A method for preparing a dual-antibody composition containing peony comprises the following steps:

[0024] (1) selecting dry, mildew-free, high-quality peony bark, crushing it into a particle size of 40-60 mesh, and adding 100 g of the crushed peony bark powder into 1 L of acetic acid-sodium acetate buffer solution with a pH value of 4.5-5.5 to prepare a suspension;

[0025] (2) adding 2 g of a composite enzyme preparation composed of cellulase, hemicellulase and pectinase in a weight ratio of 3:1.5:1 to the suspension, stirring well and performing enzymolysis reaction at 45±2° C. for 2 hours, stirring regularly during the reaction to ensure that the enzyme is in full contact with the substrate, to obtain an enzymolysis solution;

[0026] (3) adding 12 L of 75 wt% ethanol to the enzymatic hydrolyzate, mixing well, and extracting in a reflux device for 1.5 hours, so that the ethanol is continuously circulated by reflux to fully extract paeonol from the peony bark residue; then cooling to room temperature, removing the residue, and recovering the filtrate;

[0027] (4) subjecting the filtrate to vacuum distillation, collecting the distillate, crystallizing and filtering to obtain a crystal, and drying to obtain the product.

[0028] Example 2

[0029] A method for preparing a dual-antibody composition containing peony comprises the following steps:

[0030] (1) selecting dry, mildew-free, high-quality peony bark, crushing it into a particle size of 40-60 mesh, and adding 100 g of the crushed peony bark powder into 2 L of acetic acid-sodium acetate buffer solution with a pH value of 4.5-5.5 to prepare a suspension;

[0031] (2) adding 5 g of a composite enzyme preparation composed of cellulase, hemicellulase and pectinase in a weight ratio of 3:1.5:1 to the suspension, stirring well and performing enzymolysis reaction at 45±2° C. for 2 hours, stirring regularly during the reaction to ensure that the enzyme and the substrate are in full contact, to obtain an enzymolysis solution;

[0032] (3) adding 16 L of 75 wt% ethanol to the enzymatic hydrolyzate, mixing well, and extracting in a reflux device for 1.5 hours, so that the ethanol is continuously circulated by reflux to fully extract paeonol from the peony bark residue; then cooling to room temperature, removing the residue, and recovering the filtrate;

[0033] (4) subjecting the filtrate to vacuum distillation, collecting the distillate, crystallizing and filtering to obtain a crystal, and drying to obtain the product.

[0034] Example 3

[0035] A method for preparing a dual-antibody composition containing peony comprises the following steps:

[0036] (1) selecting dry, mildew-free, high-quality peony bark, crushing it into a particle size of 40-60 mesh, and adding 100 g of the crushed peony bark powder into 1.5 L of acetic acid-sodium acetate buffer solution with a pH value of 4.5-5.5 to prepare a suspension;

[0037] (2) adding 2.5 g of a composite enzyme preparation composed of cellulase, hemicellulase and pectinase in a weight ratio of 3:1.5:1 to the suspension, stirring well and performing enzymolysis reaction at 45±2° C. for 2 hours, stirring regularly during the reaction to ensure that the enzyme is in full contact with the substrate, to obtain an enzymolysis solution;

[0038] (3) adding 15 L of 75 wt% ethanol to the enzymatic hydrolyzate, mixing well, and extracting in a reflux device for 1.5 hours, so that the ethanol is continuously circulated by reflux to fully extract paeonol from the peony bark residue; then cooling to room temperature, removing the residue, and recovering the filtrate;

[0039] (4) subjecting the filtrate to vacuum distillation, collecting the distillate, crystallizing and filtering to obtain a crystal, and drying to obtain the product.

[0040] Example 4

[0041] A method for preparing a dual-antibody composition containing peony comprises the following steps:

[0042] (1) selecting dry, mildew-free, high-quality peony bark, crushing it into a particle size of 40-60 mesh, and adding 100 g of the crushed peony bark powder into 1.5 L of acetic acid-sodium acetate buffer solution with a pH value of 4.5-5.5 to prepare a suspension;

[0043] (2) adding 3 g of a composite enzyme preparation composed of cellulase, hemicellulase and pectinase in a weight ratio of 3:1.5:1 to the suspension, stirring well and performing enzymolysis reaction at 45±2° C. for 2 hours, stirring regularly during the reaction to ensure that the enzyme and the substrate are in full contact, to obtain an enzymolysis solution;

[0044] (3) adding 15 L of 75 wt% ethanol to the enzymatic hydrolyzate, mixing well, and extracting in a reflux device for 1.5 hours, so that the ethanol is continuously circulated by reflux to fully extract paeonol from the peony bark residue; then cooling to room temperature, removing the residue, and recovering the filtrate;

[0045] (4) subjecting the filtrate to vacuum distillation, collecting the distillate, crystallizing and filtering to obtain a crystal, and drying to obtain the product.

[0046] Comparative Example 1

[0047] A method for preparing a dual-antibody composition containing peony, compared with Example 3, the only difference is that: the composite enzyme preparation in step (2) is composed of cellulase and hemicellulase in a weight ratio of 3:2.5.

[0048] Comparative Example 2

[0049] A method for preparing a dual-antibody composition containing peony, compared with Example 3, the only difference is that the composite enzyme preparation in step (2) is composed of cellulase and pectinase in a weight ratio of 3:2.5.

[0050] Comparative Example 3

[0051] A method for preparing a dual-antibody composition containing peony, compared with Example 3, the only difference is that: the composite enzyme preparation in step (2) is composed of hemicellulase and pectinase in a weight ratio of 4.5:1.

[0052] Comparative Example 4

[0053] A method for preparing a dual-antibody composition containing peony, compared with Example 3, the only difference is that: the composite enzyme preparation in step (2) is composed of hemicellulase and pectinase in a weight ratio of 1.5:4.

[0054] Comparative Example 5

[0055] A method for preparing a dual-antibody composition containing peony, compared with Example 3, the only difference is that: the composite enzyme preparation in step (2) is composed of cellulase, hemicellulase and pectinase in a weight ratio of 3:1:1.

[0056] Comparative Example 6

[0057] A method for preparing a dual-antibody composition containing peony, compared with Example 3, the only difference is that the composite enzyme preparation in step (2) is composed of cellulase, hemicellulase and pectinase in a weight ratio of 3:2:1.

[0058] Comparative Example 7

[0059] A method for preparing a dual-antibody composition containing peony, which is different from Example 3 in that cellulase is used to replace the complex enzyme preparation in step (2).

[0060] Comparative Example 8

[0061] A method for preparing a dual-antibody composition containing peony, compared with Example 3, the only difference is: pure water is used to replace the acetic acid-sodium acetate buffer solution with a pH value of 4.5-5.5 in step (1).

[0062] The peony ol crystals collected in the above Examples 1-4 and Comparative Examples 1-8 were collected respectively, the weight of the peony ol crystals in each solution was weighed, and the content of peony ol was measured by UV-visible spectrophotometry. The results are shown in Table 1.

[0063] Table 1 Paeony phenol crystal weight and paeony phenol content

[0064]

[0065]

[0066] As can be seen from Table 1, the preparation method of the dual-antibody composition containing peony of the present invention decomposes the components such as cellulose, hemicellulose and pectin in the cell wall and intercellular matrix of peony bark through the synergistic action of cellulase, hemicellulase and pectinase, destroys the cell wall structure, and makes it easier to release the paeonol in the cell, thereby improving the extraction efficiency and shortening the extraction time.

[0067] The above is only a preferred embodiment of the present invention, and the protection scope of the present invention is not limited to the above embodiments. All technical solutions under the concept of the present invention belong to the protection scope of the present invention. It should be pointed out that for ordinary technicians in this technical field, some improvements and modifications without departing from the principle of the present invention should also be regarded as the protection scope of the present invention.

Claims

1. A method for preparing a dual-antibody composition containing peony, characterized in that: The following steps are included: (1) adding the crushed Paeonia suffruticosa root powder into a buffer solution to prepare a suspension; (2) adding the composite enzyme preparation to the suspension, stirring thoroughly, and performing enzymolysis reaction at 40-50° C. for 1-3 hours to obtain an enzymolysis solution; (3) adding ethanol to the enzymatic hydrolyzate, mixing well, extracting in a reflux device for 1-2 hours, then cooling to room temperature, removing the residue, and recovering the filtrate; (4) subjecting the filtrate to vacuum distillation, collecting the distillate, crystallizing and filtering to obtain a crystal, and drying to obtain the product.

2. The method for preparing the double-antibody composition containing peony according to claim 1, characterized in that: In step (1), the buffer solution is an acetic acid-sodium acetate buffer solution, and its pH value is 4.5-5.

5.

3. The method for preparing the double-antibody composition containing peony according to claim 2, characterized in that: In step (1), the solid-liquid ratio of peony bark powder to buffer solution is 1g:(10-20ml).

4. The method for preparing the double-antibody composition containing peony according to claim 1, characterized in that: In step (2), the composite enzyme preparation is composed of cellulase, hemicellulase and pectinase in a weight ratio of 3:1.5:

1.

5. The method for preparing the double-antibody composition containing peony according to claim 4, characterized in that: In step (2), the added amount of the complex enzyme preparation is 2-5% of the weight of the peony bark powder.

6. The method for preparing the double-antibody composition containing peony according to claim 1, characterized in that: In step (3), the material-liquid ratio of the enzymatic hydrolysate to ethanol is 1 ml:(8-12 ml).

7. The method for preparing the double-antibody composition containing peony according to claim 6, characterized in that: In step (3), the concentration of ethanol is 60-80%.

8. A dual-antibody composition containing peony, characterized in that: The composition is prepared by the method for preparing the double-antibody composition containing peony according to any one of claims 1 to 7.

9. An application of a dual-antibody composition containing peony, characterized in that: The dual-antibiotic composition containing peony as claimed in claim 8 is added to cosmetics to prepare cosmetics with antioxidant and anti-aging effects.

10. The use of the double-antibody composition containing peony according to claim 9, characterized in that: The dosage form of the cosmetic is water-based, emulsion, paste, powder or oily.

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