Microcapsule bead embedded with multi-element active ingredients and having anti-oxidation effect, preparation method and application

Abstract

The invention belongs to the technical field of peptide, microorganism and food processing, and discloses a micro-capsule bead embedded with multi-element active ingredients and having an anti-oxidation effect, a preparation method and application. The microcapsule bead comprises the following raw materials in parts by weight: 1-10 parts of collagen peptide, 1-5 parts of sodium alginate, 1-10 parts of complex gum, 1-2 parts of gelatin, 1-5 parts of tea polyphenol, 1-10 parts of lycium barbarum polysaccharide, 1-5 parts of bifidobacterium lactis, 1-5 parts of glycerol and purified water. According to the invention, a water-in-oil type three-layer embedding sustained-release system is constructed, and the activities of the probiotics and the collagen peptide are successfully and properly protected in the processes of processing, storage and gastrointestinal tract transmission, so that the probiotics and the collagen peptide can reach an action site in a complete and active state.

Description

Technical Field

[0001] The present invention belongs to the technical field of peptide, microorganism and food processing, and in particular to a microcapsule bead which embeds multiple active ingredients and has antioxidant effect, and a preparation method and application thereof. Background Art

[0002] Probiotics refer to living microorganisms that, when ingested in sufficient quantities, produce health benefits to the host, including improving the structure of intestinal flora, enhancing intestinal barrier function, regulating immune responses, and promoting digestion and absorption of nutrients. They are added as food additives to various functional foods, such as probiotic beverages, yogurt, milk powder, dietary supplements, etc., to increase the health value of the products. These products are designed to improve consumers' intestinal health and overall health through the intake of probiotics. Probiotics can produce a variety of antioxidant enzymes, such as superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px), which can directly act on free radicals such as superoxide anion radicals and hydrogen peroxide (H2O2), converting them into harmless water and oxygen, thereby reducing the accumulation of free radicals in the body and reducing the damage to cells caused by oxidative stress. For example, Bifidobacterium lactis secretes SOD during its growth and reproduction in the intestine, effectively removing superoxide anion radicals from the surrounding environment.

[0003] Collagen peptides are small molecule peptides obtained after collagen has been treated by enzymatic or acid hydrolysis. They are usually composed of 2 to 10 amino acids, and their relative molecular mass is generally between 1000 and 10000 Daltons. It is a hydrolyzate of collagen, and has better solubility, absorbability and biological activity while retaining some of the characteristics of collagen. The amino acid composition of collagen peptides is similar to that of collagen, and is rich in glycine, proline, hydroxyproline, etc. However, due to its small molecule, it is easier to be digested and absorbed by the human body, and can quickly pass through the intestinal wall into the blood circulation and be absorbed and utilized by cells. Collagen peptides contain a variety of antioxidant amino acid residues, such as glycine, proline, hydroxyproline, etc. These amino acid residues can provide hydrogen atoms to combine with free radicals, thereby converting free radicals into stable molecules and reducing the damage of free radicals to cells. For example, in the skin, collagen peptides can remove free radicals generated by ultraviolet radiation, such as hydroxyl radicals, superoxide anion radicals, etc., thereby slowing down the oxidative aging process of the skin. Collagen peptides are widely used in the food industry. For example, collagen peptides can be added to functional drinks such as sports drinks and energy drinks to supplement consumers with collagen consumed by exercise or daily activities, and at the same time help to restore and repair muscles after exercise; as one of the main ingredients of beauty capsules, they can be taken orally to supplement collagen for the human body, increase skin elasticity and luster, reduce wrinkles, and have obvious beauty effects; they can also be added to yogurt, which will not only not affect the taste and flavor of yogurt, but also improve the nutritional value of yogurt. The probiotics in yogurt work synergistically with collagen peptides, which helps to promote intestinal absorption, enhance immunity, and have beauty effects.

[0004] Lycium barbarum polysaccharide is a water-soluble polysaccharide extracted from wolfberry, which has a variety of important biological activities and health functions. Lycium barbarum polysaccharide has a strong antioxidant capacity and can remove free radicals in the body, such as hydroxyl free radicals, superoxide anion free radicals, etc., reduce the damage of free radicals to cells, and thus delay the aging process. As a natural antioxidant and immunomodulator, it is widely used in health foods, such as Lycium barbarum polysaccharide capsules, oral liquids, etc., for enhancing immunity, delaying aging, improving vision, etc.

[0005] Research on the effect of Lycium barbarum polysaccharides on the growth of probiotics in the intestine (Effects of Lycium barbarum polysaccharides on Escherichia coli, Bifidobacterium and Lactobacillus in the Intestinal Tract of H22 Hepatocellular Carcinoma Mice, Deng Xiangliang, Lin Qianqian, Luo Xia, et al., Food Science and Technology, 2019, 44(12): 247-252) has reported that Lycium barbarum polysaccharides may regulate intestinal Bifidobacterium indirectly. One of the theoretical bases supporting this speculation is that intestinal probiotics and harmful bacteria compete for space and nutrition in the intestinal mucosa, that is, the space-occupying effect; because Lycium barbarum polysaccharides inhibit the growth of Escherichia coli, they are beneficial to the growth of Bifidobacterium.

[0006] Tea polyphenols is a general term for a class of polyhydroxyphenol compounds contained in tea leaves. It has a rich chemical structure and a wide range of physiological activities. Tea polyphenols have strong antioxidant capacity, and its antioxidant activity is stronger than traditional antioxidants such as vitamin C and vitamin E. It can remove free radicals in the body, such as superoxide anion free radicals, hydroxyl free radicals, lipid peroxy free radicals, etc., reduce the damage of free radicals to cells, and thus delay the aging process. As a natural antioxidant, it is widely used in food preservation and processing, which can extend the shelf life of food and prevent food from oxidative deterioration. Tea polyphenols are relatively stable under acidic and neutral conditions, but are prone to oxidation reactions under alkaline conditions, resulting in changes in their structure and activity. In addition, tea polyphenols are sensitive to light, heat, oxygen, etc., and appropriate protective measures should be taken during processing and storage to prevent the degradation of its active ingredients.

[0007] Microcapsule technology protects the encapsulated substances from the influence of the external environment, such as oxidation, hydrolysis, photolysis, etc., by forming tiny capsule-like structures, thereby ensuring their effectiveness and stability. At the same time, microcapsule technology can also achieve controlled release and targeted delivery of the encapsulated substances, improving the use effect of the product. Through microcapsule technology, probiotics and collagen peptides can be encapsulated in microcapsules to cover up the bad smell of collagen peptides. At the same time, a sustained release effect can be achieved, so that probiotics and collagen peptides are protected from the influence of gastric acid and pepsin, directly enter the intestines and are absorbed, greatly improving the bioavailability of collagen peptides.

[0008] Probiotics help regulate the balance of intestinal flora and improve the intestinal microecological environment, thereby improving the intestinal absorption of nutrients. The absorption of collagen peptides in the intestine will also benefit from a good intestinal environment. The synergistic effect of the two helps to improve their respective efficacy. Products that combine probiotics and collagen peptides can not only provide collagen peptides to the skin to maintain skin elasticity and reduce wrinkles, but also regulate intestinal function through probiotics, promote nutrient absorption, improve the body's immunity, and achieve a health care effect of both internal and external cultivation. In terms of stability, probiotics need to maintain a certain activity during processing and storage, while collagen peptides have a certain stability. The two can be compatible with each other under appropriate formulas and process conditions and coexist in products; in terms of solubility, collagen peptides have good water solubility, and probiotics can also remain stable in an aqueous environment in the presence of a suitable carrier or protective agent, which allows them to be evenly mixed in products of different dosage forms such as liquids, gels or solids. This type of combination product is suitable for people who are concerned about skin health, such as female consumers, and for people who hope to improve their overall health by regulating intestinal flora, and has broad market prospects.

[0009] Single probiotic products commonly sold on the market mainly focus on regulating the balance of intestinal flora, enhancing intestinal barrier function, and promoting digestion and absorption, etc., in terms of intestinal health. They mainly attract people who are concerned about intestinal health, digestive function, and immune regulation, such as those who often suffer from constipation or diarrhea, and those with low immunity. Single collagen peptide products commonly sold on the market focus on providing building materials for skin, bones and other tissues to maintain their structure and function, such as increasing skin elasticity and promoting bone strength, and are more popular among people who pay attention to skin care (such as women's anti-wrinkle needs). In compound products, probiotics can improve the intestinal microecological environment, enhance the intestinal absorption capacity of nutrients including collagen peptides, and enable collagen peptides to be more efficiently absorbed and utilized by the human body, thereby improving intestinal health while enhancing the efficacy of collagen peptides on skin, bones, etc., achieving a synergistic effect of 1+1>2. For example, probiotics can promote the production of short-chain fatty acids in the intestines, change the pH of the intestinal environment, facilitate the dissociation and absorption of collagen peptides, and thus more effectively promote skin collagen synthesis and reduce wrinkle formation. The improvement effect on the skin is more significant than that of a single product.

[0010] In a single probiotic product, probiotics face the difficulty of maintaining their activity during storage, and are easily inactivated by environmental factors such as temperature, humidity, and oxygen. In a single collagen peptide product, although collagen peptides are relatively stable, they may also degrade or denature under some extreme conditions. In compound products, collagen peptides can provide physical protection for probiotics to a certain extent, reducing the impact of the external environment on probiotics. At the same time, the metabolites of probiotics or their own regulatory effects on environmental pH are also conducive to the maintenance of a stable structure of collagen peptides, thereby improving the overall stability of the product, extending the shelf life, and reducing the risk of loss during production, storage, and transportation. This is an advantage that is difficult to achieve with a single added product. For example, in a high temperature environment, collagen peptides may form a wrapping or isolation layer to reduce the direct effect of heat on probiotics and ensure the activity of probiotics.

[0011] Through searching, no patent publication documents related to the patent application of the present invention have been found. Summary of the invention

[0012] The purpose of the present invention is to overcome the deficiencies in the prior art and provide a microcapsule bead encapsulating multiple active ingredients and having antioxidant effects, a preparation method and an application thereof.

[0013] The technical solution adopted by the present invention to solve its technical problem is:

[0014] A microcapsule bead with multiple active ingredients embedded and having antioxidant effect, comprising the following raw materials in parts by weight:

[0015] 1~10 parts of collagen peptide, 1~5 parts of sodium alginate, 1~10 parts of composite glue, 1~2 parts of gelatin, 1~5 parts of tea polyphenols, 1~10 parts of wolfberry polysaccharides, 1~5 parts of Bifidobacterium lactis, 1~5 parts of glycerin, and purified water.

[0016] Furthermore, the microcapsule beads include the following raw materials in parts by weight: 5 parts of collagen peptide, 1 part of sodium alginate, 3 parts of composite glue, 1 part of gelatin, 2 parts of tea polyphenols, 3 parts of wolfberry polysaccharides, 1 part of Bifidobacterium lactis, 2 parts of glycerol, and an appropriate amount of purified water.

[0017] Furthermore, the collagen peptides include 1 part of bonito elastin peptide, 1 part of tilapia collagen peptide, 1 part of cod collagen peptide, 1 part of Rousselot collagen peptide, and 1 part of collagen tripeptide, and the above parts are all parts by weight.

[0018] Furthermore, the composite gum includes 1 part of carrageenan, 1 part of gellan gum, 1 part of pectin, 1 part of guar gum and 1 part of xanthan gum, and the above parts are all parts by weight.

[0019] Furthermore, the extraction method of Lycium barbarum polysaccharide is as follows:

[0020] (1) Select high-quality and full wolfberry dried fruits, crush and grind them, pass them through an 80-mesh sieve, mix the wolfberry powder with pure water at a solid-liquid ratio of 1:35 (g:ml), and perform water extraction at 90°C for 2.5 h. Extract twice, combine the two extracts, filter the extracts with gauze, and place the filtrate in a centrifuge for centrifugation at 3600 rpm for 10 min.

[0021] (2) taking the supernatant, pouring it into a round-bottom flask, and performing rotary evaporation at 65°C to reduce its volume to 1 / 5 of its original volume to obtain a concentrated solution;

[0022] (3) The concentrated solution was subjected to alcohol precipitation treatment with anhydrous ethanol for 12 h. After alcohol precipitation, the solution was centrifuged at 3600 rpm for 15 min, and crude protein was removed by Sevage method to obtain crude polysaccharide.

[0023] (4) The crude polysaccharide is placed in a dialysis bag and dialyzed for 7 hours. The obtained polysaccharide is vacuum freeze-dried to obtain a powdered polysaccharide, thereby obtaining wolfberry polysaccharide.

[0024] The method for preparing the microcapsule beads as described above comprises the following steps:

[0025] (1) Compound tea polyphenols, wolfberry polysaccharides, and lactobacillus powder as prebiotics;

[0026] (2) Mix the collagen peptide, the prebiotics prepared in step (1) and purified water at a material-water ratio of 1:3 g / ml, dissolve at room temperature to obtain a microcapsule core solution, and keep warm at 40° C. for later use;

[0027] (3) Mix sodium alginate and purified water at a material-water ratio of 1:14.8 g / ml, heat at 85°C to dissolve the sodium alginate solution, and keep at 40°C for later use;

[0028] (4) Mix the composite glue and glycerin (half the total weight of glycerin) and purified water at a material-water ratio of 1:14.6 (g / ml), heat at 85°C to dissolve the composite glue solution, and keep it at 40°C for later use;

[0029] (5) Mix the sodium alginate solution of step (3) and the composite glue solution of step (4), heat and mix at 65° C. to obtain a microcapsule wall solution, and keep the mixture at 40° C. for later use;

[0030] (6) Mix gelatin, glycerol (half the total weight of glycerol) and purified water at a material-water ratio of 1:14.6 (g / ml), heat and mix at 65°C to obtain a microcapsule shell solution, and keep warm at 40°C for later use;

[0031] The microcapsule core solution in step (2), the microcapsule wall solution in step (5) and the microcapsule shell solution in step (6) are simultaneously added into a pellet machine with three concentric nozzles, wherein the solutions sprayed from the nozzles are respectively microcapsule core, microcapsule wall and microcapsule shell solutions from the inside to the outside. The pellet machine is started and the nozzle spray speed is adjusted until the three nozzles spray out microcapsule bead components simultaneously. The microcapsule beads are directly dropped into white oil which is precooled to 4° C. and circulated. The microcapsule beads formed by pelletization are placed in a drum air drying machine and dried at 25° C. for 8 hours to reduce the moisture content to less than 3%, thereby obtaining microcapsule beads.

[0032] The microcapsule beads as described above are used as and / or in the preparation of medicines and / or health foods for preventing or treating antioxidant and anti-aging diseases.

[0033] The advantages and positive effects achieved by the present invention are:

[0034] 1. The present invention constructs an oil-in-water three-layer embedding sustained-release system, which successfully protects the activity of probiotics and collagen peptides during processing, storage and gastrointestinal transmission, so that they can reach the site of action in a complete and active state. More importantly, this system promotes the synergistic effect between probiotics and collagen peptides. During the colonization and metabolism of probiotics in the intestine, they can optimize the intestinal microecological environment and promote the absorption and utilization of collagen peptides by intestinal cells; while collagen peptides can provide probiotics with suitable attachment sites and nutrients, helping the growth and reproduction of probiotics. The two cooperate and complement each other, so as to more efficiently exert multiple physiological effects such as improving intestinal health, enhancing the body's immunity, and promoting skin repair and regeneration.

[0035] 2. The present invention innovatively adopts a composite wall material system, which effectively solves the problem of poor stability when sodium alginate is used alone as a wall material. Sodium alginate is a commonly used embedding material. When used alone, the interaction between molecular chains is relatively weak. Under complex environmental conditions, such as different ionic strengths, temperature changes, and mechanical stress, structural dissociation and destruction are prone to occur, which leads to leakage and loss of activity of the embedded material. The present invention forms an interwoven and stable network structure with sodium alginate by introducing a specific cross-linking agent or an auxiliary polymer compatible therewith. This network structure can enhance the mechanical strength and chemical stability of the wall material, and effectively resist the impact of external environmental factors on the embedding system.

[0036] 3. The probiotic composite microcapsule beads prepared by the present invention exhibit certain acid resistance, bile salt resistance and oxygen resistance. In the acidic environment of gastric acid (pH about 1.5 - 3.5), the wall material of the microcapsule beads can remain intact, preventing gastric acid from degrading the internal collagen peptides. Thanks to the special chemical composition and structural arrangement of the wall material, it can maintain a certain charge balance and intermolecular force under acidic conditions, avoiding structural collapse caused by processes such as protonation. When the microcapsule beads enter the intestine, even if faced with erosion by high concentrations of bile salts (such as sodium cholate, sodium deoxycholate, etc., with a concentration of up to 0.3% - 2%), the wall material still has sufficient tolerance to ensure that the probiotics and collagen peptides will not be destroyed by bile salts or released prematurely.

[0037] 4. The patent of the present invention constructs a three-layer encapsulation sustained-release system of oil-in-water type, which is effective in protecting the activity of probiotics and collagen peptides. In this system architecture, the inner layer uses hydrophilic materials to initially encapsulate probiotics and collagen peptides. This measure can effectively maintain the stability of the two and resist the interference of adverse external factors. The middle layer is further wrapped with hydrophobic materials, which greatly reduces the erosion of the core components by factors such as oxygen, moisture and gastric acid. The outer oil phase layer not only enhances the tolerance of the entire system in the gastrointestinal environment, but also can achieve precise controlled release of the contents. Through this designed three-layer encapsulation structure, the activity of probiotics and collagen peptides is successfully protected during processing, storage and gastrointestinal transmission, so that they can reach the site of action in a complete and active state. More importantly, this system promotes the synergistic effect between probiotics and collagen peptides. During the colonization and metabolism process in the intestine, probiotics can optimize the intestinal microecological environment and promote the absorption and utilization of collagen peptides by intestinal cells; while collagen peptides can provide suitable attachment sites and nutrients for probiotics, helping the growth and reproduction of probiotics. The two work together and complement each other, thus more efficiently exerting multiple physiological effects such as improving intestinal health, enhancing the body's immunity, and promoting skin repair and regeneration. BRIEF DESCRIPTION OF THE DRAWINGS

[0038] Figure 1 This is a graph showing the number of live bacteria after simulated gastric juice digestion in the present invention;

[0039] Figure 2 This is a graph of the number of viable bacteria after digestion of simulated intestinal fluid in the present invention;

[0040] Figure 3 This is a graph showing changes in SOD in mouse skin tissue in the present invention;

[0041] Figure 4 This is a graph showing changes in MDA in mouse skin tissue in the present invention;

[0042] Figure 5 This is a graph showing changes in GSH-Px in mouse skin tissue in the present invention;

[0043] Figure 6 This is a graph showing HYP changes in mouse skin tissue in the present invention. DETAILED DESCRIPTION

[0044] The present invention will be further described below in conjunction with the embodiments. The following embodiments are descriptive rather than restrictive, and the protection scope of the present invention cannot be limited by the following embodiments.

[0045] The raw materials used in the present invention, unless otherwise specified, are conventional commercially available products; the methods used in the present invention, unless otherwise specified, are conventional methods in the art.

[0046] The various experimental operations involved in the specific embodiments are all routine techniques in the art. For parts not specially annotated in this document, ordinary technicians in the art can implement them by referring to various commonly used reference books, scientific and technological literature or related instructions, manuals, etc. before the filing date of this invention.

[0047] A microcapsule bead with multiple active ingredients embedded and having antioxidant effect, comprising the following raw materials in parts by weight:

[0048] 1~10 parts of collagen peptides, 1~5 parts of sodium alginate, 1~10 parts of composite glue, 1~2 parts of gelatin, 1~5 parts of tea polyphenols, 1~10 parts of wolfberry polysaccharides, 1~5 parts of Bifidobacterium lactis, 1~5 parts of glycerin, and appropriate amount of purified water.

[0049] Preferably, the microcapsule beads include the following raw materials in parts by weight: 5 parts of collagen peptide, 1 part of sodium alginate, 3 parts of composite glue, 1 part of gelatin, 2 parts of tea polyphenols, 3 parts of wolfberry polysaccharides, 1 part of Bifidobacterium lactis, 2 parts of glycerol, and an appropriate amount of purified water. Preferably, the collagen peptide includes 1 part of bonito elastin peptide, 1 part of tilapia collagen peptide, 1 part of cod collagen peptide, 1 part of Rousselot collagen peptide, and 1 part of collagen tripeptide, and the above parts are all parts by weight.

[0050] Preferably, the composite gum includes 1 part of carrageenan, 1 part of gellan gum, 1 part of pectin, 1 part of guar gum and 1 part of xanthan gum, and the above parts are all by weight.

[0051] Preferably, the method for extracting wolfberry polysaccharides is as follows:

[0052] (1) Select high-quality and full wolfberry dried fruits, crush and grind them, pass them through an 80-mesh sieve, mix the wolfberry powder with purified water at a solid-liquid ratio of 1:35 (g:ml), and perform water extraction at 90°C for 2.5 h. Extract twice, combine the two extracts, filter the extracts with gauze, and place the filtrate in a centrifuge for centrifugation at 3600 rpm for 10 min.

[0053] (2) taking the supernatant, pouring it into a round-bottom flask, and performing rotary evaporation at 65°C to reduce its volume to 1 / 5 of its original volume to obtain a concentrated solution;

[0054] (3) The concentrated solution was subjected to alcohol precipitation treatment with anhydrous ethanol for 12 hours; after alcohol precipitation, the solution was centrifuged at 3600 rpm for 15 minutes, and crude protein was removed by Sevage method to obtain crude polysaccharide;

[0055] (4) The crude polysaccharide is placed in a dialysis bag and dialyzed for 7 hours. The obtained polysaccharide is vacuum freeze-dried to obtain a powdered polysaccharide, thereby obtaining wolfberry polysaccharide.

[0056] The method for preparing the microcapsule beads as described above comprises the following steps:

[0057] (1) Compound tea polyphenols, wolfberry polysaccharides, and lactobacillus powder as prebiotics;

[0058] (2) Mix the collagen peptide, the prebiotics prepared in step (1) and purified water at a material-water ratio of 1:3 g / ml, dissolve at room temperature to obtain a microcapsule core solution, and keep warm at 40° C. for later use;

[0059] (3) Mix sodium alginate and purified water at a material-water ratio of 1:14.8 g / ml, heat at 85°C to dissolve the sodium alginate solution, and keep at 40°C for later use;

[0060] (4) Mix the composite glue and glycerin (half the total weight of glycerin) and purified water at a material-water ratio of 1:14.6 (g / ml), heat at 85°C to dissolve the composite glue solution, and keep it at 40°C for later use;

[0061] (5) Mix the sodium alginate solution of step (3) and the composite glue solution of step (4), heat and mix at 65° C. to obtain a microcapsule wall solution, and keep the mixture at 40° C. for later use;

[0062] (6) Mix gelatin, glycerol (half the total weight of glycerol) and purified water at a material-water ratio of 1:14.6 (g / ml), heat and mix at 65°C to obtain a microcapsule shell solution, and keep warm at 40°C for later use;

[0063] The microcapsule core solution in step (2), the microcapsule wall solution in step (5) and the microcapsule shell solution in step (6) are simultaneously added into a pellet machine with three concentric nozzles, wherein the solutions sprayed from the nozzles are respectively microcapsule core, microcapsule wall and microcapsule shell solutions from the inside to the outside. The pellet machine is started and the nozzle spray speed is adjusted until the three nozzles spray out microcapsule bead components simultaneously. The microcapsule beads are directly dropped into white oil which is precooled to 4° C. and circulated. The microcapsule beads formed by pelletization are placed in a drum air drying machine and dried at 25° C. for 8 hours to reduce the moisture content to less than 3%, thereby obtaining microcapsule beads.

[0064] The microcapsule beads as described above are used as and / or in the preparation of medicines and / or health foods for preventing or treating antioxidant and anti-aging diseases.

[0065] Specifically, the relevant preparation and detection examples are as follows:

[0066] Example 1

[0067] A microcapsule bead with multiple active ingredients embedded and having antioxidant effect, comprising the following raw materials in parts by weight:

[0068] 10 parts of collagen peptides, 1 part of sodium alginate, 1 part of composite glue, 1 part of gelatin, 1 part of tea polyphenols, 1 part of wolfberry polysaccharides, 1 part of Bifidobacterium lactis, 2 parts of glycerol, and appropriate amount of purified water.

[0069] The collagen peptides include 1 part of bonito elastin peptides, 1 part of tilapia collagen peptides, 1 part of cod collagen peptides, 1 part of Rousselot collagen peptides, and 1 part of collagen tripeptide.

[0070] The composite gum comprises 1 part of carrageenan, 1 part of gellan gum, 1 part of pectin, 1 part of guar gum and 1 part of xanthan gum.

[0071] The above parts are all parts by weight.

[0072] The extraction method of wolfberry polysaccharide is as follows:

[0073] (1) Select high-quality and full wolfberry dried fruits, crush and grind them, pass them through an 80-mesh sieve, mix the wolfberry powder with purified water at a solid-liquid ratio of 1:35, and perform water extraction at 90°C for 2.5 h. Extract twice, combine the two extracts, filter the extracts with gauze, and place the filtrate in a centrifuge for centrifugation at 3600 rpm for 10 min.

[0074] (2) Take the supernatant, pour it into a round-bottom flask, and perform rotary evaporation at 65°C to reduce its volume to 1 / 5 of its original volume to obtain a concentrated solution;

[0075] (3) The concentrated solution was subjected to alcohol precipitation with anhydrous ethanol for 12 hours. After alcohol precipitation, the solution was centrifuged at 3600 rpm for 15 minutes, and the crude protein was removed by the Sevage method to obtain crude polysaccharides.

[0076] (4) The crude polysaccharide is placed in a dialysis bag and dialyzed for 7 hours. The obtained polysaccharide is vacuum freeze-dried to obtain a powdered polysaccharide, thereby obtaining wolfberry polysaccharide.

[0077] The method for preparing the above-mentioned microcapsule beads encapsulating multiple active ingredients and having antioxidant effects comprises the following steps:

[0078] (1) Compound tea polyphenols, wolfberry polysaccharides, and lactobacillus powder as prebiotics;

[0079] (2) Mix the collagen peptides, the prebiotics from step (1) and purified water at a material-water ratio of 1:3 g / ml, dissolve at room temperature to obtain a microcapsule core solution, and keep warm at 40° C. for later use;

[0080] (3) Mix sodium alginate and purified water at a material-water ratio of 1:14.8 g / ml, heat at 85°C to dissolve the sodium alginate solution, and keep at 40°C for later use;

[0081] (4) Mix the composite glue and glycerin (half the total weight of glycerin) and purified water at a material-water ratio of 1:14.6 (g / ml), heat at 85°C to dissolve the composite glue solution, and keep it at 40°C for later use;

[0082] (5) Mix the sodium alginate solution of step (3) and the composite glue solution of step (4), heat and mix at 65° C. to obtain a microcapsule wall solution, and keep the mixture at 40° C. for later use;

[0083] (6) Mix gelatin, glycerol (half the total weight of glycerol) and purified water at a material-water ratio of 1:14.6 (g / ml), heat and mix at 65°C to obtain a microcapsule shell solution, and keep warm at 40°C for later use;

[0084] The microcapsule core solution in step (2), the microcapsule wall solution in step (5) and the microcapsule shell solution in step (6) are simultaneously added into a pellet machine with three concentric nozzles, wherein the solutions sprayed from the nozzles are respectively microcapsule core, microcapsule wall and microcapsule shell solutions from the inside to the outside. The pellet machine is started and the nozzle spray speed is adjusted until the three nozzles spray out microcapsule bead components simultaneously. The microcapsule beads are directly dropped into white oil which is precooled to 4° C. and circulated. The microcapsule beads formed by pelletization are placed in a drum air drying machine and dried at 25° C. for 8 hours to reduce the moisture content to less than 3%, thereby obtaining microcapsule beads.

[0085] Example 2

[0086] A microcapsule bead with multiple active ingredients embedded and having antioxidant effect comprises the following raw materials in parts by weight: 10 parts of collagen peptide, 2 parts of sodium alginate, 2 parts of composite glue, 1 part of gelatin, 1 part of tea polyphenols, 2 parts of wolfberry polysaccharides, 1 part of Bifidobacterium lactis, 2 parts of glycerol, and an appropriate amount of purified water.

[0087] The collagen peptides include 1 part of bonito elastin peptides, 1 part of tilapia collagen peptides, 1 part of cod collagen peptides, 1 part of Rousselot collagen peptides, and 1 part of collagen tripeptide.

[0088] The composite gum comprises 1 part of carrageenan, 1 part of gellan gum, 1 part of pectin, 1 part of guar gum and 1 part of xanthan gum.

[0089] The above parts are all parts by weight.

[0090] The method for extracting wolfberry polysaccharides is the same as that in Example 1.

[0091] The preparation method of the above-mentioned microcapsule beads encapsulating multiple active ingredients and having antioxidant effects has the same steps as Example 1.

[0092] Example 3

[0093] A microcapsule bead with multiple active ingredients embedded and having antioxidant effect, comprising the following raw materials in parts by weight:

[0094] 5 parts of collagen peptide, 1 part of sodium alginate, 3 parts of composite glue, 1 part of gelatin, 2 parts of tea polyphenols, 3 parts of wolfberry polysaccharides, 1 part of Bifidobacterium lactis, 2 parts of glycerol, and appropriate amount of purified water.

[0095] The collagen peptides include 1 part of bonito elastin peptides, 1 part of tilapia collagen peptides, 1 part of cod collagen peptides, 1 part of Rousselot collagen peptides, and 1 part of collagen tripeptide.

[0096] The composite gum comprises 1 part of carrageenan, 1 part of gellan gum, 1 part of pectin, 1 part of guar gum and 1 part of xanthan gum.

[0097] The above parts are all parts by weight.

[0098] The method for extracting wolfberry polysaccharides is the same as that in Example 1.

[0099] The preparation method of the above-mentioned microcapsule beads encapsulating multiple active ingredients and having antioxidant effects has the same steps as Example 1.

[0100] Comparative Example 1

[0101] The same as Example 3, except that collagen peptides are not embedded, and the following raw materials are included in parts by weight: 1 part of sodium alginate, 3 parts of composite glue, 1 part of gelatin, 2 parts of tea polyphenols, 3 parts of wolfberry polysaccharides, 1 part of Bifidobacterium lactis, 2 parts of glycerol, and an appropriate amount of purified water.

[0102] The composite gum comprises 1 part of carrageenan, 1 part of gellan gum, 1 part of pectin, 1 part of guar gum and 1 part of xanthan gum.

[0103] The above parts are all parts by weight.

[0104] The method for extracting wolfberry polysaccharides is the same as that in Example 1.

[0105] The above-mentioned method for preparing microcapsule beads with multiple active ingredients and antioxidant effects is the same as that in Example 1, except that the specific steps of step (2) are:

[0106] (2) Mix the prebiotics prepared in step (1) and purified water at a material-water ratio of 1:3 g / ml, dissolve at room temperature to obtain a microcapsule core solution, and keep warm at 40° C. for later use;

[0107] Comparative Example 2

[0108] The same as Example 3, except that: Bifidobacterium lactis is not embedded, and the following raw materials are included in parts by weight: 5 parts of collagen peptide, 2 parts of tea polyphenols, 3 parts of wolfberry polysaccharides, 1 part of sodium alginate, 3 parts of composite glue, 1 part of gelatin, 2 parts of glycerol, and an appropriate amount of purified water.

[0109] The composite gum comprises 1 part of carrageenan, 1 part of gellan gum, 1 part of pectin, 1 part of guar gum and 1 part of xanthan gum.

[0110] The collagen peptides include 1 part of bonito elastin peptides, 1 part of tilapia collagen peptides, 1 part of cod collagen peptides, 1 part of Rousselot collagen peptides, and 1 part of collagen tripeptide.

[0111] The above parts are all parts by weight.

[0112] The method for extracting wolfberry polysaccharides is the same as that in Example 1.

[0113] The above-mentioned method for preparing microcapsule beads with multiple active ingredients and antioxidant effects is the same as that in Example 1, except that the specific steps of step (1) are:

[0114] (1) Mix tea polyphenols and wolfberry polysaccharides and use them as prebiotics.

[0115] Experimental Example 1 Evaluation of the Tolerance of Bifidobacterium lactis to the Simulated Gastrointestinal Fluid Environment in Vitro by Adding Tea Polyphenols and Lycium barbarum Polysaccharides

[0116] The detection method is:

[0117] The live bacteria count is based on the bifidobacterium count method in GB4789.34-2016 “National Food Safety Standard Food Microbiology Examination Bifidobacterium Examination” with slight modifications.

[0118] 1. Evaluation of the tolerance of Bifidobacterium lactis to simulated gastric fluid environment by adding tea polyphenols and wolfberry polysaccharides

[0119] Artificial gastric juice: After sterilizing 0.1 mol / L potassium phosphate buffer, adjust the pH to 2.5 with standard hydrochloric acid titration solution, add pepsin with a mass concentration of 10 g / L, mix well and pass through a 0.22 μm sterile membrane to obtain in vitro simulated artificial gastric juice.

[0120] 3g of Bifidobacterium lactis powder (naked bacteria group) and 3g of prebiotics (1g of Bifidobacterium lactis powder, 1g of Lycium barbarum polysaccharide, 1g of tea polyphenols, prebiotic group) were added to 300mL of liquid MRS medium and cultured at 37℃ for 24h. 2.0mL of bacterial solution was added to 20.0mL of simulated gastric juice in vitro to simulate gastric juice digestion process under anaerobic conditions of 37℃ and 90rpm. Samples were taken at 0h, 1h, 2h and 3h for live bacteria count and survival rate was calculated.

[0121] The results are as follows Figure 1 As shown in the figure, at a pH of 1.5 and after 3 hours of digestion, the number of live probiotics in the naked bacteria group dropped to 0, while the number of live Bifidobacterium lactis in the prebiotic group was still high, with a survival rate of 87.25%, and its tolerance to simulated gastric juice was significantly better than that of the naked bacteria group (p < 0.05). This may be related to the adhesion of cell wall polysaccharides, which reduces the bacteria's exposure to acid stress and absorption of H⁺, while activating the activity of glutamate dehydrogenase and improving the ability of Bifidobacterium lactis to resist gastric acid. Studies have shown that the prebiotic group can increase the survival rate of Bifidobacterium lactis in microcapsule strains in gastric juice, facilitate its proliferation, and enhance the antioxidant and anti-aging health care effects.

[0122] 2. Evaluation of the tolerance of Bifidobacterium lactis to simulated intestinal fluid environment by adding tea polyphenols and wolfberry polysaccharides

[0123] Artificial intestinal fluid: After sterilizing 0.1 mol / L potassium phosphate buffer, adjust the pH to 6.8 with 0.1 mol / L sodium hydroxide solution, add pancreatic enzyme with a final mass concentration of 10 g / L and porcine bile salt with a final mass concentration of 3 g / L, mix well and pass through a 0.22 μm sterile membrane to obtain in vitro simulated artificial intestinal fluid.

[0124] 3g of Bifidobacterium lactis powder (naked bacteria group) and 3g of prebiotics (1g of Bifidobacterium lactis powder, 1g of Lycium barbarum polysaccharide, 1g of tea polyphenols, prebiotic group) were added to 300mL of liquid MRS medium and cultured at 37℃ for 24h. 2.0mL of bacterial solution was added to 80.0mL of simulated intestinal fluid in vitro to simulate intestinal fluid digestion process under anaerobic conditions of 37℃ and 90rpm. Samples were taken at 0h, 1h, 2h and 6h for live bacteria count and survival rate was calculated.

[0125] The results are as follows Figure 2 As shown in the results, after 6 hours of digestion, the survival rates of Bifidobacterium lactis in the naked bacteria group and the prebiotic group decreased to 45.61% and 58.33%, respectively. After 1 hour of digestion in the intestinal fluid, the Bifidobacterium lactis in the prebiotic group proliferated fastest, with a survival rate of 92.57%. Although it dropped to 79.66% after 2 hours, it was still higher than the 63.53% in the naked bacteria group. The results showed that the polysaccharides and tea polyphenols in prebiotics can significantly improve the tolerance of Bifidobacterium lactis in intestinal fluid (p < 0.05), which may be related to the polysaccharide structure and glutamate dehydrogenase activity.

[0126] Test Example 2 Determination of collagen peptide embedding rate in microcapsule beads

[0127] 0.5 g of the microcapsule beads of Examples 1 to 3 and Comparative Example 2 were weighed respectively, and the collagen peptide content was determined according to the detection method for the proportion of collagen peptides with a relative molecular mass of less than 10,000 in GB31645-2018 "National Food Safety Standard-Collagen Peptide" (high performance size exclusion chromatography), with slight modifications. The results are shown in Table 1.

[0128] According to the results in Table 1, it can be seen that Example 3 has the best encapsulation effect. Its protein content reaches 29.66±0.22g / 100g, far exceeding Examples 1-2 and Comparative Example 2; although the proportion of collagen peptides with a relative molecular mass of less than 10,000Da is similar to that of other samples, the overall encapsulation efficiency is more outstanding; the encapsulation rate is as high as 98.56±0.01%, which is far ahead of other samples, and can efficiently encapsulate the target, reduce losses, and ensure activity and stability. It has great advantages under the combined effect of the three aspects.

[0129] Table 1 Determination of collagen peptide embedding rate in microcapsule beads

[0130]

[0131] Test Example 3 Evaluation of Antioxidant Efficacy

[0132] The microcapsule beads prepared in Example 1-3 and Comparative Example 1-2 were used as test samples. The ability to scavenge DPPH free radicals was determined by referring to the method of Moure et al. The ability to scavenge hydroxyl free radicals was determined by referring to the method of Sun.＋ The capacity was determined according to the method of Ozgen. All samples were tested three times in parallel and the average value was taken.

[0133] Determination of DPPH free radical scavenging ability: Accurately weigh 0.0197g (0.05 mmol) of DPPH and dissolve it in 250mL of anhydrous ethanol to prepare a DPPH working solution with a concentration of 0.2mmol / L. Then, the test samples were prepared with 50% ethanol by volume to prepare sample ethanol solutions with mass concentrations of 0.001, 0.01, 0.1, 0.4, 0.8, 1.2, 1.6, and 2.0 mg / mL. Vitamin C ethanol solution was prepared in the same way. 100μL of different sample solutions and 100μL of DPPH working solution were added to a 96-well plate. After mixing evenly, it was kept away from light at room temperature for 30 minutes, and its absorbance at 517nm was determined using an enzyme marker. Vitamin C was used as the positive control, and each experiment was repeated 3 times. The calculation formula for DPPH free radical scavenging rate is:

[0134]

[0135] Wherein: A1 is the absorbance of sample solution + DPPH working solution; A2 is the absorbance of 50% by volume ethanol + DPPH working solution; A3 is the absorbance of sample solution + 50% by volume ethanol.

[0136] Determination of hydroxyl radical scavenging ability: Prepare the sample solution into corresponding concentration gradients (50, 100, 125, 150, 175, 200, 400, 600, 800 mg / L), take 2.0 mL of sample solution of different concentrations in a test tube, add 2.0 mL of 9.0 mmol / L FeSO4 solution, then add 1.0 mL of 8.8 mmol / L H2O2 solution, shake well, let stand for 10 min, then add 2.0 mL of 9.0 mmol / L salicylic acid-ethanol solution, shake well, and place in a constant temperature water bath at 37°C for 30 min. Use an ultraviolet spectrophotometer to measure the absorbance at a wavelength of 510 nm, and use vitamin C as a positive control to calculate the hydroxyl radical scavenging rate of sample solutions of different concentrations. The hydroxyl radical scavenging rate calculation formula is:

[0137]

[0138] Where: A0 is the absorbance of the blank control solution; A i A is the absorbance after adding the sample; j is the background absorbance of the sample solution.

[0139] Clear ABTS +Capacity determination: weigh 1g (1.82 mmol) of ABTS and dissolve it in deionized water to prepare a final concentration of 7.4 mmol / L ABTS working solution; weigh 0.5g of potassium persulfate and dissolve it in deionized water to prepare a final concentration of 2.6 mmol / L potassium persulfate working solution; mix equal volumes of ABTS working solution and potassium persulfate working solution, and react in the dark for 12 hours to obtain ABTS + Then, prepare sample ethanol solution and Vc ethanol solution as above, add 40 μL of different sample solution and 160 μL of ABTS+ working solution to the 96-well plate, mix well, react at room temperature in the dark for 6 min, and measure the absorbance of the solution at 734 nm with an ELISA reader. + The clearance rate was calculated as follows:

[0140]

[0141] Where: A1 is sample solution + ABTS + Absorbance of working solution; A2 is deionized water + ABTS + A3 is the absorbance of the sample solution + deionized water.

[0142] Table 2 Evaluation of antioxidant efficacy

[0143]

[0144] According to the results of Table 2, from the data in the table, in terms of antioxidant related indicators, each sample presents different performances. In DPPH radical scavenging rate, embodiment 3 reaches 84.57 ± 0.11%, significantly higher than 59.99 ± 0.13% of comparative example 1, 60.56 ± 0.12% of comparative example 2, and also more advantageous compared with 80.21 ± 0.11% of embodiment 1 and 81.55 ± 0.09% of embodiment 2, indicating that the ability of embodiment 3 to remove DPPH radicals is stronger. For hydroxyl radical scavenging rate, embodiment 3 is 85.23 ± 0.07%, significantly higher than 80.59 ± 0.07% of embodiment 1, 82.11 ± 0.09% of embodiment 2 and 50.51 ± 0.05% of comparative example 1 and 52.35 ± 0.09% of comparative example 2, reflecting that embodiment 3 is outstanding in removing hydroxyl radicals. As for the ABTS free radical scavenging rate, 78.56±0.01% of Example 3 is close to 78.54±0.03% of Example 1, slightly higher than 75.06±0.03% of Example 2, and much higher than 43.06±0.01% of Comparative Example 1 and 43.56±0.11% of Comparative Example 2.

[0145] From the perspective of the comprehensive free radical scavenging rate index, Example 3 performs excellently in terms of antioxidant capacity, showing obvious advantages over the comparative examples and other examples, and has a stronger ability to scavenge free radicals and resist oxidation.

[0146] At the same time, by comparing Comparative Examples 1 to 3 and Comparative Examples 1 to 2, it can also be seen that the collagen peptide and Bifidobacterium lactis in the present invention have a synergistic effect, which can synergistically improve the relevant properties of the prepared microcapsule beads. In particular, when the mass ratio of collagen peptide to Bifidobacterium lactis is 5:1, the relevant properties of the prepared microcapsule beads can be significantly improved.

[0147] Test Example 4: Evaluation of anti-aging efficacy

[0148] Animal model establishment: The SPF-grade male healthy Balb / c mice used in this experiment were provided by Henan Sikebes Biotechnology Co., Ltd., with a body weight controlled between 18 and 20 g, and the license number was SCXK (Yu) 2020-0005. During the experiment, the mice were kept in a constant environment, the temperature was maintained at 20-25 ° C, the relative humidity was controlled at 50 ± 5%, and a 12-h light-dark cycle was followed. To ensure that the mice adapted to the experimental environment, they were adaptively raised for one week. 50 mice were randomly divided into 5 groups, 10 in each group, set as experimental group 1 (adding microcapsule beads prepared in Example 3), experimental group 2 (adding microcapsule beads prepared in Comparative Example 1), experimental group 3 (adding microcapsule beads prepared in Comparative Example 2), blank control group, and model control group. The model control group and mice in each experimental group were subcutaneously injected with 1000 mg / kg of D-galactose in the back of the neck every day to establish a subacute aging model in mice, and the blank control group was subcutaneously injected with an equal amount of 0.9% saline in the back of the neck. Drugs were administered at the same time as modeling: ① Blank control group (10 mice): gavage with 0.1 ml / 10 g of 0.9% saline. ② Model control group (10 mice): subcutaneous injection of D-galactose 1000 mg / kg at the back of the neck, and gavage with 0.1 ml / 10 g of 0.9% saline. ③ Experimental group (30 mice): The experimental group was divided into 3 groups, each with 10 mice. Each group received a subcutaneous injection of D-galactose 1000 mg / kg at the back of the neck every day. 0.5 g of the sample in Example 3 and Comparative Example 1-2 was dissolved in 10 mL of distilled water to prepare a suspension. The 3 groups of mice were gavaged with 40 mg·kg of the suspension. -1 ·d -1 After 6 weeks of sterile environment, the mice were killed by cervical dislocation, and the skin between the two shoulder blades on the back was taken, with an area of ​​3 cm × 3 cm, and the skin tissue was homogenized.

[0149] The activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) and the contents of hydroxyproline (HYP) and malondialdehyde (MDA) in mouse skin tissue were determined.

[0150] Changes of SOD in mouse skin tissue, see Figure 3 . Changes in MDA in mouse skin tissue, see Figure 4 . Changes in GSH-Px in mouse skin tissue, see Figure 5 . Changes in HYP in mouse skin tissue, see Figure 6 . As can be seen from the results in the figure, after continuous subcutaneous injection of D-galactose into the neck of mice for 42 days, the MDA activity in the aging model group mice was 24U / mgprot, which was significantly higher than the 14U / mgprot of the blank control group mice. The GSH-Px, SOD and HYP were 150U / mgprot, 222U / mgprot and 0.35U / mgprot, respectively, which were significantly lower than the 257U / mgprot, 260U / mgprot and 0.61U / mgprot of the blank control group, proving that the mouse modeling was successful in this experiment. The MDA content in the skin tissue of mice in the experimental group 1 was 14U / mgprot, which was significantly lower than the 24U / mgprot, 19U / mgprot and 18U / mgprot of the model control group, experimental group 2 and experimental group 3 (P < 0.05), indicating that the microcapsule beads prepared in Example 3 can effectively reduce the generation of MDA, an oxidative damage product in mice. At the same time, the GSH-Px, SOD and HYP activities of the experimental group 1 were 212, 259 U / mgprot, and 0.59 ug / ml, respectively, which were significantly higher than those of the model control group and other experimental groups (P < 0.05), indicating that Example 3 can significantly enhance the antioxidant enzyme activity of mice and increase the HYP content related to collagen synthesis, thereby confirming that the microcapsule beads prepared in Example 3 have a significant anti-aging effect, while the effects of Comparative Examples 1 and 2 are not as good as those of Example 3, and the indicators of the blank control group are within the normal physiological range.

[0151] At the same time, by comparing Comparative Example 3 and Comparative Examples 1 to 2, it can also be seen that the collagen peptide and Bifidobacterium lactis in the present invention have a synergistic effect, which can synergistically improve the relevant properties of the prepared microcapsule beads. In particular, when the mass ratio of collagen peptide to Bifidobacterium lactis is 5:1, the relevant properties of the prepared microcapsule beads can be significantly improved.

[0152] Test Example 5 Storage stability of microcapsule beads

[0153] The microcapsule beads were placed in a sealed sterile glass bottle and then stored at 4°C for 18 days. Samples were taken every 3 days. The collagen peptide embedding rate in the microcapsule beads was detected according to the method described in Experimental Example 2 to determine the storage stability of the microcapsule beads.

[0154] The experimental results of the collagen peptide content in the microcapsule beads are shown in Table 3. According to the experimental results shown in Table 3, after storage for 18 days, the collagen peptide content in the microcapsule beads prepared in Example 3 is still about 97.06, which is the highest content in all embodiments. By comparing the experimental results, it is proved that there is a significant synergistic stabilization effect between Lycium barbarum polysaccharides, tea polyphenols, Bifidobacterium lactis and collagen peptides. At the same time, it is shown that the microcapsule beads have a good protective effect on collagen peptides. The appropriate addition amount combination can ensure that the product maintains good quality during the storage period.

[0155] Table 3 Microcapsule beads collagen peptide content test

[0156] Test Example 6 Synergistic Effect of the Present Invention

[0157] In the present invention, there is a synergistic effect between the addition amount of Lycium barbarum polysaccharides, tea polyphenols, Bifidobacterium lactis, collagen peptides, sodium alginate and composite glue, as shown in Table 4. According to the results in Table 4, it can be seen that Lycium barbarum polysaccharides and tea polyphenols in the present invention promote the synergistic effect between probiotics and collagen peptides. During the colonization and metabolism process in the intestine, probiotics can optimize the intestinal microecological environment and promote the absorption and utilization of collagen peptides by intestinal cells; while collagen peptides can provide suitable attachment sites and nutrients for probiotics, and help the growth and reproduction of probiotics. The two cooperate with each other and complement each other. At the same time, a composite wall material system is adopted to effectively solve the problem of poor storage stability when sodium alginate is used alone as a wall material.

[0158] Table 4 Synergistic effect of each ingredient

[0159]

[0160] At the same time, by comparing Example 3 and Comparative Examples 1 to 2, it can also be seen that the collagen peptide and Bifidobacterium lactis in the present invention have a synergistic effect, which can synergistically improve the relevant properties of the prepared microcapsule beads. In particular, when the mass ratio of collagen peptide to Bifidobacterium lactis is 5:1, the relevant properties of the prepared microcapsule beads can be significantly improved.

[0161] Although the embodiments of the present invention are disclosed for illustrative purposes, those skilled in the art will appreciate that various substitutions, changes and modifications are possible without departing from the spirit and scope of the present invention and the appended claims. Therefore, the scope of the present invention is not limited to the contents disclosed in the embodiments.

Claims

1. A microcapsule bead with multiple active ingredients embedded and having antioxidant effect, characterized in that: The invention comprises the following raw materials in parts by weight: 1~10 parts of collagen peptide, 1~5 parts of sodium alginate, 1~10 parts of composite glue, 1~2 parts of gelatin, 1~5 parts of tea polyphenols, 1~10 parts of wolfberry polysaccharides, 1~5 parts of Bifidobacterium lactis, 1~5 parts of glycerin, and purified water.

2. The microcapsule beads according to claim 1, characterized in that: The microcapsule beads include the following raw materials in parts by weight: 5 parts of collagen peptide, 1 part of sodium alginate, 3 parts of composite glue, 1 part of gelatin, 2 parts of tea polyphenols, 3 parts of wolfberry polysaccharides, 1 part of Bifidobacterium lactis, 2 parts of glycerol, and purified water.

3. The microcapsule beads according to claim 1, characterized in that: The collagen peptides include 1 part of bonito elastin peptide, 1 part of tilapia collagen peptide, 1 part of cod collagen peptide, 1 part of Rousselot collagen peptide, and 1 part of collagen tripeptide, and the above parts are all parts by weight.

4. The microcapsule beads according to claim 1, characterized in that: The composite gum comprises 1 part of carrageenan, 1 part of gellan gum, 1 part of pectin, 1 part of guar gum and 1 part of xanthan gum, and the above parts are all parts by weight.

5. The microcapsule beads according to any one of claims 1 to 4, characterized in that: The extraction method of wolfberry polysaccharide is as follows: (1) Select dried wolfberry fruit, crush and grind it, pass it through an 80-mesh sieve, mix the wolfberry powder with purified water at a solid-liquid ratio of 1:35 (g:ml), and perform water extraction at 90°C for 2.5 h. Extract twice, combine the two extracts, filter the extract with gauze, and place the filtrate in a centrifuge for centrifugation at 3600 rpm for 10 min. (2) taking the supernatant, pouring it into a round-bottom flask, and performing rotary evaporation at 65°C to reduce its volume to 1 / 5 of its original volume to obtain a concentrated solution; (3) The concentrated solution was subjected to alcohol precipitation treatment with anhydrous ethanol for 12 h. After alcohol precipitation, the solution was centrifuged at 3600 rpm for 15 min, and crude protein was removed by Sevage method to obtain crude polysaccharide. (4) The crude polysaccharide is placed in a dialysis bag and dialyzed for 7 hours. The obtained polysaccharide is vacuum freeze-dried to obtain a powdered polysaccharide, thereby obtaining wolfberry polysaccharide.

6. The method for preparing microcapsule beads according to any one of claims 1 to 5, characterized in that: The steps include: (1) Compound tea polyphenols, wolfberry polysaccharides, and lactobacillus powder as prebiotics; (2) Mix the collagen peptide, the prebiotics prepared in step (1) and purified water at a material-water ratio of 1:3 g / ml, dissolve at room temperature to obtain a microcapsule core solution, and keep warm at 40° C. for later use; (3) Mix sodium alginate and purified water at a material-water ratio of 1:14.8 g / ml, heat at 85°C to dissolve the sodium alginate solution, and keep at 40°C for later use; (4) Mix the composite glue and glycerol (half the total weight of glycerol) and purified water at a material-water ratio of 1:14.6 (g / ml), heat at 85°C to dissolve the composite glue solution, and keep it at 40°C for later use; (5) Mix the sodium alginate solution of step (3) and the composite glue solution of step (4), heat and mix at 65° C. to obtain a microcapsule wall solution, and keep the mixture at 40° C. for later use; (6) Mix gelatin, glycerol (half the total weight of glycerol) and purified water at a material-water ratio of 1:14.6 (g / ml), heat and mix at 65°C to obtain a microcapsule shell solution, and keep warm at 40°C for later use; The microcapsule core solution in step (2), the microcapsule wall solution in step (5) and the microcapsule shell solution in step (6) are simultaneously added into a pellet machine with three concentric nozzles, wherein the solutions sprayed from the nozzles are respectively microcapsule core, microcapsule wall and microcapsule shell solutions from the inside to the outside. The pellet machine is started and the nozzle spray speed is adjusted until the three nozzles spray out microcapsule bead components simultaneously. The microcapsule beads are directly dropped into white oil which is precooled to 4° C. and circulated. The microcapsule beads formed by pelletization are placed in a drum air drying machine and dried at 25° C. for 8 hours to reduce the moisture content to less than 3%, thereby obtaining microcapsule beads.

7. Use of the microcapsule beads according to any one of claims 1 to 5 as and / or in the preparation of medicines and / or health foods for preventing or treating antioxidant and anti-aging diseases.

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