Ginseng product with functions of resisting oxidation, resisting aging, prolonging life and improving skin and preparation process of ginseng product

Abstract

The invention discloses a ginseng product capable of resisting oxidation, resisting aging, prolonging life and improving skin and a preparation process of the ginseng product. According to the ginseng product, rhizoma atractylodis macrocephalae, radix astragali, radix angelicae sinensis, radix aucklandiae, pericarpium citri reticulatae, radix glycyrrhizae, arillus longan, fresh ginger, fructus jujubae and semen ziziphi spinosae are adopted as basic raw materials, a precious medicinal material ginseng is soaked with alcohol and then decocted with water to obtain a ginseng extract, on the one hand, a nanometer bolete product is prepared, and the ginseng extract is added and boiled, so that the cell DNA repairing capacity can be improved; and then adding old honey containing fructus perillae to modify the mixture. Meanwhile, the ginseng extract, the nanometer bolete product and the old honey containing the perilla seeds have a synergistic effect, can effectively delay senescence, have anti-allergy and repairing effects, relieve skin allergy and provide a natural protection barrier for the skin.

Description

Technical Field

[0001] The invention relates to the field of biomedicine, and in particular to a ginseng product with the functions of anti-oxidation, anti-aging, longevity and skin improvement and a preparation process thereof. Background Art

[0002] Aging is an inevitable spontaneous process of the body. When the body ages, the most intuitive thing to observe is skin aging, which includes sagging, dryness, and wrinkling of the skin, and degenerative changes in elastic fibers and collagen fibers at the microscopic level. The skin is an important protective barrier for organisms, protecting the body from physical and chemical damage and bacterial invasion, while also playing a role in immune monitoring and hormone secretion. When the body's skin ages, these functions will be affected. The molecular basis of skin aging includes oxidative stress, chronic inflammation, DNA damage, decreased cellular DNA repair ability, and telomere loss. Among them, oxidative stress is one of the main factors inducing skin aging, which can directly lead to mitochondrial dysfunction, cell damage, triggering activation of the complement system and inflammation, and then inducing macrophage infiltration of the skin. Excessive accumulation of reactive oxygen species (ROS) can cause DNA damage, induce skin inflammatory responses, reduce antioxidant enzymes, activate nuclear factor κB (NF-κB) and activator protein 1 (AP-1) to inhibit collagen production, and increase collagen degradation by matrix metalloproteinases (MMPs), which have a negative impact on the skin and cause aging.

[0003] The parasitic flora in the intestine constitutes a microbial barrier. When the body ages, the intestinal barrier will be damaged and the intestinal flora will also be disturbed. Glucose, fructose and galactose are essential monosaccharides in the diet. Excessive carbohydrates cause abnormal mitochondrial oxidative metabolism of glucose, leading to the production of reactive oxygen species (ROS) and oxidative stress in the body. D-gal and glucose are isomers, which can be converted into glucose in normal metabolism and participate in glucose metabolism. Compared with glucose, D-gal is a more powerful oxidant that can induce oxidative stress.

[0004] According to traditional Chinese medicine, ginseng has the effect of replenishing qi and nourishing the five internal organs. Through pharmacological research, it is found that various ginsenosides in ginseng are active ingredients with various effects. Ginseng is known as the "king of herbs" and has many pharmacological effects such as immune regulation and anti-aging. At present, more than 60 kinds of ginsenosides have been isolated from ginseng, and they all have their unique physiological activities. Such as inhibiting tumor cell growth, enhancing the body's immunity, and anti-fatigue.

[0005] The present invention uses Atractylodes macrocephala, Radix Astragali, Radix Angelicae Sinensis, Radix Aucklandiae, Pericarpium Citri Reticulatae, Licorice, Longan pulp, Ginger, Jujube, Semen Ziziphi Spinosae as basic raw materials, takes precious medicinal material ginseng, soaks it in alcohol, and then decocts it in water to obtain ginseng extract. On the other hand, a nano-shaped boletus product is prepared, and the ginseng extract is added and boiled to enhance the cell DNA repair ability, and then old honey containing Perilla seeds is added to modify it, and Perilla seeds are rich in nutrients. At the same time, the ginseng extract, the nano-shaped boletus product and the old honey containing Perilla seeds have a synergistic effect, and can effectively delay aging. Summary of the invention

[0006] The purpose of the present invention is to overcome the deficiencies of the prior art and provide a ginseng product with the functions of anti-oxidation, anti-aging and longevity and a preparation process thereof.

[0007] In a first aspect, the present invention provides a preparation process of a ginseng product having antioxidant, anti-aging and longevity effects, and the specific preparation steps are as follows:

[0008] S1. Wash and dry 3-6g of Atractylodes macrocephala, 1-3g of Astragalus, 5-6g of Angelica sinensis, 2-4g of Aucklandia lappa, 6-8g of Tangerine peel, 3-5g of Licorice, and 4-5g of Longan pulp; peel and slice 4-6g of ginger, and pit 15-18g of jujube; stir-fry 6-8g of Ziziphus jujuba seeds and crush them into 80-mesh coarse powder. After combining the above drugs, add 8-10 times the amount of distilled water, soak for 8-11h before the first decoction, decoct 2-3 times, each time for 60-70min, combine the filtrate and concentrate to 120-150ml for standby use; this process is the raw material synthesis step, and the compatibility of various medicinal materials can be improved by soaking and decoction.

[0009] S2. Take 8-10g of the precious medicinal material ginseng, first add 100mL of 70-80% ethanol to soak for 2-4h, then filter to obtain the alcohol soaking solution, then take out the ginseng and add 8-10 times the amount of distilled water thereto, repeat the same steps for decoction and extraction 3 times, the decoction time is 90min, 60min, and 60min respectively, combine the alcohol soaking solution and the 3 decoction solutions to obtain a filtrate, and then concentrate the filtrate to 16-20ml to obtain the ginseng extract for later use; this step of adding ethanol to soak for 2-4h extraction can improve the purity of ginseng, reduce the content of reactive oxygen ROS, and thus alleviate DNA damage.

[0010] S3, weigh 10-13g of dried porcini products, add distilled water at a ratio of 30:1mL / g, soak in water at 30-50℃ for 15-20min to make the dried products absorb water and swell, transfer to a wall breaking machine for 5-8s, extract in water at 80-90℃ for 1-3h, filter, extract the residue twice at a solid-liquid ratio of 10:1mL / g, extract in water at 90-100℃ for 1-3h, filter, combine the two filtrates and concentrate them to 10-15ml, add anhydrous ethanol to the concentrated filtrate to make the ethanol concentration of the whole system reach 75-80%, transfer the filtrate containing ethanol to The mixture was placed in a 4°C refrigerator for 12-15 hours, centrifuged at 4000-6000 r / min for 2-5 minutes, and the precipitate was freeze-dried to obtain a solid powder. 2-3 g of the solid powder was roasted at 300°C for 2 hours, and after it was naturally cooled, it was dissolved in 80-100 mL of water and 0.1-0.5 g of hyaluronic acid was added. After stirring at 60°C for 3 hours, it was freeze-dried to obtain a nano-shaped boletus product; the dry boletus product added in this step has a variety of beauty and skin care effects. It is rich in vitamins, proteins, antioxidants and minerals, which can improve skin quality and delay skin aging. The nano-shaped boletus product has good compatibility and is more conducive to being added to the paste to improve the skin's moisturizing type. The boletus product was purchased from Yongren Yesen Mushroom Industry Co., Ltd.

[0011] S4, take 16-19g honey, add 4-6g perilla seeds, stir evenly, add 8-10 times the amount of distilled water and boil for 30-40min, filter to remove impurities and concentrate into a clear paste with a relative density of 1.15 (60℃), make old honey containing perilla seeds, add before collecting the paste; perilla seeds and honey together have the effect of delaying aging, whitening and slimming. Perilla seeds were purchased from Bozhou Ruantang Pharmaceutical Co., Ltd.; honey was purchased from Beijing Qipsida Technology Co., Ltd.;

[0012] S5. Take 35 ml of the product obtained in step S1 and 8 ml of the product obtained in step 2, place them in a fully automatic evaporator, first heat them with high heat until the medicinal liquid boils, remove the foam produced after boiling at any time, then take 1 g of the product obtained in step S3 and add it thereto. When the medicinal liquid becomes concentrated, switch to low heat to continue concentrating it into a clear paste with a relative density of 1.0-1.2 (60°C), take 8-12 g of the old honey containing perilla seeds in step S4 and add it to the clear paste, stir while adding and reduce the heat, heat until it falls in sheets when picked up with a thin stick, and when the paste can be pulled out with 2 cm of white silk by pinching it between the index finger and thumb, collect the paste to obtain a ginseng product.

[0013] Preferably: in step S1 of the present invention, 3g of Atractylodes macrocephala, 1g of Astragalus membranaceus, 6g of Angelica sinensis, 4g of Aucklandia lappa, 6g of Tangerine peel, 5g of Licorice root, and 5g of Longan pulp are washed; 6g of ginger is peeled and sliced;

[0014] Preferably: in step S1 of the present invention, 18g of jujube is pitted; 8g of sour jujube kernels are fried and crushed into 80 mesh coarse powder, the above drugs are combined, 8 times the amount of distilled water is added, soaked for 11 hours, and decocted 3 times, each decocting time is 70 minutes;

[0015] Preferably: in step S2 of the present invention, 8g of precious medicinal material ginseng is firstly added to 80ml of 80% ethanol for immersion for 2h and then filtered to obtain an alcohol immersion solution, then 10 times the amount of distilled water is added to the ginseng, and decocted and extracted 3 times, the decocting time is 90min, 60min, and 60min respectively, and the alcohol immersion solution and the 3 decoction solutions are combined to obtain a filtrate;

[0016] Preferably: in step S3 of the present invention, 13 g of the dried porcini product is weighed and distilled water is added at a ratio of 30:1 mL / g. After soaking in warm water for 15 min to allow the dried product to absorb water and swell, the dried product is transferred to a wall-breaking machine for 8 s, extracted with hot water at 120° C. for 1 h, and filtered;

[0017] Preferably: in step S3 of the present invention, anhydrous ethanol is added to the concentrated filtrate to make the ethanol concentration of the whole system reach 75%, the filtrate containing ethanol is transferred to a 4°C refrigerator and placed for 15 hours, 6000r / min, centrifuged for 2 minutes, and the precipitate is freeze-dried to obtain a solid powder;

[0018] Preferably: in step S5 of the present invention, 19 g of honey is taken, 4 g of alpine perilla seeds are added, stirred evenly with a magnetic stirrer, 8 times the amount of distilled water is added and boiled for 30 min, filtered to remove impurities and concentrated into a clear paste with a relative density of 1.15 (60° C.), that is, the old honey containing perilla seeds is prepared;

[0019] In a second aspect, the present invention provides a ginseng product with antioxidant, anti-aging and longevity effects prepared by the above-mentioned preparation process of the ginseng product with antioxidant, anti-aging and longevity effects.

[0020] The benefits of the present invention are:

[0021] 1. In step S2 of the present invention, adding ethanol and soaking for 2-4 hours before water extraction is beneficial to improving the purity of ginseng and reducing the content of reactive oxygen species (ROS), thereby alleviating DNA damage.

[0022] 2. The present invention adopts nano-sized dried bovine liver preparation and old honey containing perilla seeds, which have a synergistic effect, can play a role in immune monitoring and hormone secretion, delay aging, whiten and lose weight.

[0023] 3. The old honey containing perilla seeds, ginseng extract and nano-boletus products in the ginseng product prepared by the present invention work synergistically, have anti-allergic and repairing effects, relieve skin allergies, and provide a natural protective barrier for the skin.

[0024] 4. Adding hyaluronic acid after roasting dried porcini can improve the smoothness of yeast cells to a certain extent, significantly increase the activity of GSH-Px in serum, and significantly reduce the MDA content in brain tissue.

[0025] 5. The raw materials of the present invention are easily available and the preparation method is simple. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 The bar graphs are of the MDA content and GSH-Px activity in the brain tissue of mice acted upon by the ginseng products prepared in Example 1 and Comparative Examples 1-4.

[0027] Figure 2 This is a SEM image of the ginseng product in Example 1 acting on mouse cells.

[0028] Figure 3 This is the SEM image of the ginseng product in Comparative Example 2 acting on mouse cells.

[0029] Figure 4 This is the SEM image of the ginseng product in Comparative Example 3 acting on mouse cells.

[0030] Figure 5 This is a bar chart comparing the skin itching experimental indicators of each group of mice. DETAILED DESCRIPTION

[0031] In order to make the technical problems, technical solutions and beneficial effects to be solved by the present invention more clearly understood, the present invention is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the content of the present invention and are not used to limit the present invention.

[0032] In the present invention, ginseng was purchased from Xi'an Andrui Biotechnology Co., Ltd., and other Chinese medicinal materials (such as Atractylodes macrocephala, Astragalus membranaceus, Angelica sinensis, costusroot, tangerine peel, licorice, longan pulp, ginger, jujube, and spinach seed) were purchased from Chongqing Baimutang Chinese Medicinal Materials Co., Ltd.

[0033] Example 1

[0034] A ginseng product with anti-oxidation, anti-aging and longevity effects, the specific preparation steps of the preparation method are as follows:

[0035] S1. Wash and dry 6g of Atractylodes macrocephala, 3g of Astragalus, 6g of Angelica sinensis, 4g of Aucklandia lappa, 8g of Tangerine peel, 5g of Licorice, and 5g of Longan pulp; peel and slice 6g of ginger, and pit 18g of jujube; stir-fry 8g of Ziziphus jujuba seeds and crush them into 80-mesh coarse powder. After combining the above drugs, add 10 times the amount of distilled water, soak for 11 hours, decoct for 3 times, each time for 70 minutes, combine the filtrate and concentrate to 150ml for standby use; this process is the raw material synthesis step, and the compatibility of various medicinal materials can be improved by soaking and decoction.

[0036] S2. Take 10g of the precious medicinal material ginseng, first add 100mL of 80% ethanol to soak for 4h, then filter to obtain the alcohol soaking solution, then take out the ginseng and add 10 times the amount of distilled water thereto, repeat the same steps for decocting and extracting 3 times, the decoction time is 90min, 60min, and 60min respectively, combine the alcohol soaking solution and the 3 decoction solutions to obtain a filtrate, and then concentrate the filtrate to 20ml to obtain the ginseng extract for later use; this step of adding ethanol soaking for 4h extraction can improve the purity of ginseng, reduce the content of reactive oxygen ROS, and thus alleviate DNA damage.

[0037] S3, weigh 13g of dried porcini products, add distilled water at a ratio of 30:1mL / g, soak in 50℃ water for 20min to make the dried products absorb water and swell, transfer to a wall-breaking machine for 8s, extract in 90℃ water for 3h, filter, extract the residue twice at a solid-liquid ratio of 10:1mL / g, extract in 100℃ water for 3h, filter, combine the two filtrates and concentrate to 15ml, add anhydrous ethanol to the concentrated filtrate to make the ethanol concentration of the whole system reach 80%, transfer the filtrate containing ethanol to 4℃ ice, and freeze. The mixture was placed in a freezer for 12 hours, centrifuged at 4000r / min for 5 minutes, and the precipitate was freeze-dried to obtain a solid powder. 3g of the solid powder was roasted at 300°C for 2 hours, dissolved in 80mL of water after natural cooling, and 0.1g of hyaluronic acid was added. After stirring at 60°C for 3 hours, the dried boletus product was freeze-dried to obtain a nano-shaped boletus product. The dried boletus product added in this step has a variety of beauty and skin care effects. It is rich in vitamins, proteins, antioxidants and minerals, which can improve skin quality and delay skin aging. The nano-shaped boletus product has good compatibility and is more conducive to being added to the paste to improve the skin's moisturizing type. The boletus product was purchased from Yongren Yesen Mushroom Industry Co., Ltd.

[0038] S4, take 19g of honey, add 6g of perilla seeds, stir evenly, add 10 times the amount of distilled water and boil for 40min, filter to remove impurities and concentrate into a clear paste with a relative density of 1.15 (60℃), make old honey containing perilla seeds, add before collecting the paste; perilla seeds and honey together have the effect of delaying aging, whitening and slimming. Perilla seeds were purchased from Bozhou Ruantang Pharmaceutical Co., Ltd.; honey was purchased from Beijing Qipsida Technology Co., Ltd.;

[0039] S5. Take 35 ml of the product obtained in step S1 and 8 ml of the product obtained in step 2, place them in a fully automatic evaporator, first heat them with high heat until the medicinal liquid boils, remove the foam produced after boiling at any time, then take 1 g of the product obtained in step S3 and add it thereto. When the medicinal liquid becomes concentrated, use low heat to continue concentrating it into a clear paste with a relative density of 1.2 (60°C), take 12 g of the old honey containing perilla seeds in step S4 and add it to the clear paste, stir while adding and reduce the heat, heat until it falls in sheets when picked up with a thin stick, and when the paste can be pulled out with 2 cm of white silk by pinching it between the index finger and thumb, collect the paste to obtain a ginseng product.

[0040] Comparative Example 1 Except that ethanol soaking is not performed in step S2, the remaining steps are the same as those of Example 1.

[0041] In Comparative Example 2, except that the precipitate is directly modified by adding sodium hyaluronate without being roasted in step S3, the remaining steps are the same as those in Example 1.

[0042] Comparative Example 3 Except that hyaluronic acid modification is not performed in step S3, the remaining steps are the same as those of Example 1.

[0043] Comparative Example 4: Except that the old honey containing perilla seeds obtained in step S4 is not added in step S5, the remaining steps are the same as those in Example 1.

[0044] Take 64 KM mice, male, weighing 20 ± 2g, 4 weeks old, after 3 days of adaptive feeding of mice, randomly take 56 mice and inject D-galactose (500mg / kg) subcutaneously in the neck and back for 4 weeks, and the gavage volume is 10mL / kg to establish a mouse aging model. The remaining 8 are blank groups, and the blank group is injected with an equal volume of normal saline. After the modeling is successful, the 56 modeling mice are randomly divided into 7 groups, namely the model group, the positive control group (vitamin C), Example 1, and the comparative example 1-4 groups. Among them, the model group is gavaged with normal saline, the positive control group is administered with a dose of 200mg / kg, and the dosage of Example 1 and the comparative example 1-4 groups is 50mg / kg, and the gavage dose is 10ml / kg, and it is continuously administered for 4 weeks. After the last administration, the mouse is removed from the eyeball to take blood, and after standing for 30min, it is centrifuged at 3000r / min for 10min, and the supernatant is aspirated and frozen at -20℃ for testing. Part of the mouse brain tissue was taken and the glutathione peroxidase (GSH-Px) activity and malondialdehyde (MDA) content in serum and brain tissue homogenate were determined according to the kit instructions. The results are shown in Tables 1, 2 and Figure 1 Reduced glutathione (GSH-Px) and malondialdehyde (MDA) detection kits were purchased from Qiyi Biotechnology (Shanghai) Co., Ltd.

[0045] Table 1 Reduced glutathione (GSH-Px) activity in mouse serum

[0046]

[0047]

[0048] Table 2 Reduced glutathione (GSH-Px) activity and malondialdehyde (MDA) content in mouse brain tissue

[0049]

[0050] The mice to which the ginseng product was continuously administered for 4 weeks were taken, and the subcutaneous connective tissue of the cerebral cortex of the mice in Example 1, Comparative Example 2, and Comparative Example 3 were respectively taken for primary cell culture. The small tissue blocks cut were inoculated into a culture bottle using the tissue block culture method. The bottle wall can be pre-coated with a thin layer of collagen to facilitate the adhesion of the tissue block to the bottle wall, so that the surrounding cells can grow outward along the bottle wall. The mouse cells were taken for SEM testing, and the experimental data of the effect of the ginseng product on the mouse cells were obtained, as shown in FIG. Figures 2 to 4 shown. Figure 1 The bar graphs are the MDA content and GSH-Px activity in the brain tissue of mice acted upon by the ginseng products prepared in Example 1 and Comparative Examples 1-4. Figure 2 This is the SEM image of the ginseng product acting on mouse cells in Example 1. Figure 3 This is the SEM image of the ginseng product acting on mouse cells in Comparative Example 2. Figure 4 The SEM image of the ginseng product acting on mouse cells in comparative example 3. Table 1 and Table 2 are respectively the malondialdehyde (MDA) content and GSH-Px activity in serum and brain tissue of mice after the ginseng product acted on mice.

[0051] GSH-Px is an endogenous anti-free radical substance that can remove excess free radicals in the body. MDA is a product of lipid peroxidation, reflecting the degree of lipid peroxidation and is often used as an important indicator for evaluating aging. Figure 1 It can be seen that compared with the normal group, the GSH-Px activity in the serum and brain tissue of mice in the model group was significantly reduced, and the MDA content in the brain tissue was significantly increased, indicating that the modeling was successful. Compared with Comparative Examples 1-4, the GSH-Px activity in the serum and brain tissue of mice in Example 1 was significantly increased, and the MDA content in the brain tissue was significantly reduced. The increase in GSH-Px activity can break the reactive oxygen species (ROS) existing in the body, so the ginseng extract obtained by soaking ginseng in ethanol and then decocting it, the nano-shaped bovine liver preparation, and the old honey containing perilla seeds have a synergistic effect, which can significantly increase the GSH-Px activity in serum and brain tissue, and significantly reduce the MDA content in brain tissue. At the same time, from Figure 2 It can be seen that the cell morphology is stable, the cells are irregularly spherical, the surface is smooth and without wrinkles, and a large number of cells are in budding reproduction. Figure 3 and Figure 4 There are a lot of wrinkles on the surface of the spherical structure, the surface is rough, the smoothness is reduced, it is irregular in shape, floccules are produced, and the cells have obvious signs of cell death. It can be seen that adding hyaluronic acid to dried boletus can improve the aging of yeast cells to a certain extent and delay the aging process.

[0052] Example 2

[0053] A ginseng product with anti-oxidation, anti-aging and longevity effects, the specific preparation steps of the preparation method are as follows:

[0054] S1. Wash and dry 3g Atractylodes macrocephala, 1g Astragalus, 5g Angelica sinensis, 2g Costus root, 6g Tangerine peel, 3g Licorice root, and 4g Longan pulp; peel and slice 4g ginger, and pit 15g jujube; stir-fry 6g Ziziphus jujuba seeds and crush them into 80-mesh coarse powder. After combining the above drugs, add 8 times the amount of distilled water, soak for 8h, decoct twice, each time for 60min, combine the filtrate and concentrate to 120ml for standby use; this process is the raw material synthesis step, and the compatibility of various medicinal materials can be improved by soaking and decoction.

[0055] S2. Take 8g of the precious medicinal material ginseng, first add 80ml of 70% ethanol to soak for 2h, then filter to obtain the alcohol soaking solution, then take out the ginseng and add 8 times the amount of distilled water thereto, repeat the same steps for decoction and extraction 3 times, the decoction time is 90min, 60min, and 60min respectively, combine to obtain a filtrate containing the alcohol soaking solution and the 3 decoction solutions, then concentrate the filtrate to 16ml to obtain the ginseng extract, and set aside; this step of adding ethanol to soak for 2h extraction can improve the purity of ginseng, reduce the content of reactive oxygen ROS, and thus alleviate DNA damage.

[0056] S3. Weigh 10g of dried porcini products, add distilled water at a ratio of 30:1mL / g, soak in 30℃ water for 15min to allow the dried products to absorb water and swell, then transfer to a wall-breaking machine for 5s, extract in 80℃ water for 1h, filter, extract the residue twice at a solid-liquid ratio of 10:1mL / g, extract in 90℃ water for 1h, filter, combine the two filtrates and concentrate them to 10ml, add anhydrous ethanol to the concentrated filtrate to make the ethanol concentration of the whole system reach 75%, and transfer the filtrate containing ethanol to a 4℃ refrigerator. The mixture was allowed to stand for 15 hours, centrifuged at 6000r / min for 2 minutes, and the precipitate was freeze-dried to obtain a solid powder. 2g of the solid powder was roasted at 300°C for 2 hours, and after cooling naturally, 0.5g of hyaluronic acid was added to 100mL of water, and the mixture was stirred at 60°C for 3 hours and freeze-dried to obtain a nano-shaped boletus product. The dried boletus product added in this step has a variety of beauty and skin care effects. It is rich in vitamins, proteins, antioxidants and minerals, which can improve skin quality and delay skin aging. The nano-shaped boletus product has good compatibility and is more conducive to being added to the paste to improve the skin's moisturizing type. The boletus product was purchased from Yongren Yesen Mushroom Industry Co., Ltd.

[0057] S4, take 16g of honey, add 4g of perilla seeds, stir evenly, add 8 times the amount of distilled water and boil for 30min, filter to remove impurities and concentrate into a clear paste with a relative density of 1.15 (60℃), make old honey containing perilla seeds, add before collecting the paste; perilla seeds and honey together have the effect of delaying aging, whitening and slimming. Perilla seeds were purchased from Bozhou Ruantang Pharmaceutical Co., Ltd.; honey was purchased from Beijing Qipsida Technology Co., Ltd.;

[0058] S5. Take 35 ml of the product obtained in step S1 and 8 ml of the product obtained in step 2, place them in a fully automatic evaporator, first heat them with high heat until the medicinal liquid boils, remove the foam produced after boiling at any time, then take 1 g of the product obtained in step S3 and add it thereto. When the medicinal liquid becomes concentrated, use low heat to continue concentrating it into a clear paste with a relative density of 1.0 (60°C), take 8 g of the old honey containing perilla seeds in step S4 and add it to the clear paste, stir while adding and reduce the heat, heat until it can be picked up with a thin stick and falls in the form of sheets, and when the paste can be pulled out by pinching it between the index finger and the thumb, the paste is collected to obtain a ginseng product.

[0059] Comparative Example 5 Except that the old honey containing perilla seeds obtained in step S4 is not added in step S5, the remaining steps are the same as those in Example 2.

[0060] Comparative Example 6 Except that the ginseng extract obtained in step S2 is not added in step S5, the remaining steps are the same as those of Example 2.

[0061] In Comparative Example 7, except that the nano-shaped boletus product obtained in step S3 is not added in step S5, the remaining steps are the same as those in Example 2.

[0062] Animal grouping: 56 Kunming mice, half male and half female, were selected and divided into 7 groups, namely blank control group, model group, loratadine group, Example 2 group, and Comparative Example 5-7 group, according to the random number table method, with 8 mice in each group. The drug administration began 3 days after drug administration and adaptive feeding of modeling. The blank control group and the model group were gavaged with 20 mL / kg of normal saline; the loratadine group was administered with 1.52 mg / kg, and the Example 2 group and Comparative Example 5-7 groups were gavaged with a dosage of 50 mg / kg and a daily gavage volume of 20 mL / kg. The above groups of mice were gavaged continuously for 7 days, once a day. One hour after the last administration, the mouse skin itch experiment was induced by intravenous injection of 0.0125% low molecular weight dextran solution into the tail vein of mice in the other groups except the blank group, and the blank group was replaced with normal saline. The successful performance of the itch experiment: the mice had a systemic itch reaction, and scratching the head with the forepaws, scratching the trunk with the back, and biting various parts of the body with the mouth were the itching indications. The results are shown in Tables 3 and Figure 5 shown.

[0063] Table 3

[0064]

[0065] Table 3 is a comparison of the skin pruritus test indicators of each group of mice. Figure 5 It can be seen that compared with the blank control group, the skin itching of the mice in the model group was aggravated, the number and duration of scratching were significantly increased, and the onset latency of the mice after intervention with the ginseng product was significantly prolonged, the number and duration of scratching were significantly reduced, and the skin itching was improved; compared with comparative examples 5-7, the onset latency of the mice in Example 2 was significantly prolonged, the number and duration of scratching were significantly reduced, and the skin itching was improved, indicating that the ginseng extract, the nano-shaped boletus product, and the old honey containing perilla seeds contained in the ginseng product prepared by the present invention have a synergistic effect, have an anti-allergic repair effect, relieve skin allergies, and provide a natural protective barrier for the skin.

Claims

1. A dried porcini product, characterized in that: The preparation method is as follows: add distilled water at a ratio of 30:1 mL / g, soak in water at 30-50°C for 15-20 min to allow the dry product to absorb water and swell, then transfer to a wall-breaking machine for 5-8 s, extract in water at 80-90°C for 1-3 h, filter, extract the filter residue twice at a solid-liquid ratio of 10:1 mL / g, extract in water at 90-100°C for 1-3 h, filter, combine the two filtrates and concentrate to 10-15 ml, add anhydrous ethanol to the concentrated filtrate to make the whole The ethanol concentration of the system reaches 75-80%, the filtrate containing ethanol is transferred to a 4°C refrigerator and allowed to stand for 12-15 hours, 4000-6000 r / min, centrifuged for 2-5 minutes, the precipitate is freeze-dried to obtain a solid powder, 2-3 g of the solid powder is roasted at 300°C for 2 hours, after natural cooling, it is dissolved in 80-100 mL of water and 0.1-0.5 g of hyaluronic acid is added, stirred at 60°C for 3 hours and freeze-dried to obtain a nano-boletus product.

2. A preparation process of a ginseng product with antioxidant, anti-aging and longevity effects, characterized in that: The specific preparation steps are as follows: S1, wash and dry 3-6g Atractylodes macrocephala, 1-3g Astragalus, 5-6g Angelica sinensis, 2-4g Aucklandia lappa, 6-8g Tangerine peel, 3-5g Licorice, and 4-5g Longan flesh; peel and slice 4-6g ginger, remove the core of 15-18g jujube; stir-fry 6-8g Ziziphus jujuba seeds and crush them into 80 mesh coarse powder. After combining the above drugs, add 8-10 times the amount of distilled water, soak for 8-11h before the first decoction, decoct 2-3 times, each decoction time is 60-70min, combine the filtrate and concentrate to 120-150ml for standby use; S2, take 8-10g of precious medicinal material ginseng, first add 80-100ml of 70-80% ethanol to soak for 2-4h, then filter to obtain alcohol soaking solution, then take out ginseng and add 8-10 times amount of distilled water to it, repeat the same steps to decoct and extract 3 times, the decoction time is 90min, 60min, and 60min respectively, combine the alcohol soaking solution and the 3 decoction solutions to obtain a filtrate, and then concentrate the filtrate to 16-20ml to obtain ginseng extract, which is set aside; S3, weighing 10-13 g of the dried boletus product according to claim 1; S4, take 16-19g of honey, add 4-6g of perilla seeds, mix well, add 8-10 times the amount of distilled water and boil for 30-40min, filter to remove impurities and concentrate into a clear paste with a relative density of 1.15 (60°C), that is, make old honey containing perilla seeds, add before collecting the paste; S5. Take 35 ml of the product obtained in step S1 and 8 ml of the product obtained in step 2, place them in a fully automatic evaporator, first heat them with high heat until the medicinal liquid boils, remove the foam produced after boiling at any time, then take 1 g of the product obtained in step S3, add it thereto, and when the medicinal liquid becomes concentrated, switch to low heat to continue concentrating it into a clear paste with a relative density of 1.0-1.2 (60°C), take 8-12 g of the old honey containing perilla seeds in step S4, add it to the clear paste, stir while adding and reduce the heat, heat until it can be picked up with a thin stick and falls in the form of sheets, and when the paste can be pulled out by pinching it between the index finger and the thumb, the paste is collected to obtain a ginseng product.

3. The preparation process of a ginseng product with antioxidant, anti-aging and longevity-prolonging effects according to claim 2, characterized in that: In the step S1, 3g of Atractylodes macrocephala, 1g of Astragalus membranaceus, 6g of Angelica sinensis, 4g of Aucklandia lappa, 6g of Tangerine peel, 5g of Licorice root, and 5g of Longan pulp are washed; and 6g of ginger is peeled and sliced.

4. The preparation process of a ginseng product with antioxidant, anti-aging and longevity-prolonging effects according to claim 2, characterized in that: In the step S1, 18g of jujube is pitted; 8g of Chinese date kernel is fried and crushed into 80 mesh coarse powder. After the above drugs are combined, 8 times amount of distilled water is added, soaked for 11 hours, and decocted for 3 times, with the decocting time of 70 minutes each time.

5. The preparation process of a ginseng product with antioxidant, anti-aging and longevity-prolonging effects according to claim 3, characterized in that: In the step S2, 8 g of precious medicinal material ginseng is taken, firstly 80 mL of 80% ethanol is added to soak for 2 hours, and then the alcohol soaking liquid is filtered, then ginseng is taken out and 10 times of distilled water is added thereto, and decocted and extracted 3 times, with the decoction time being 90 min, 60 min, and 60 min respectively, and the filtrate is combined.

6. The preparation process of a ginseng product with antioxidant, anti-aging and longevity-prolonging effects according to claim 4, characterized in that: In the step S3, anhydrous ethanol is added to the concentrated filtrate to make the ethanol concentration of the whole system reach 75%, the filtrate containing ethanol is transferred to a 4°C refrigerator and allowed to stand for 15 hours, centrifuged at 6000 r / min for 2 minutes, and the precipitate is freeze-dried to obtain a solid powder.

7. The preparation process of a ginseng product with antioxidant, anti-aging and longevity-prolonging effects according to claim 6, characterized in that: In the step S4, 19g of honey is taken, 4g of perilla seeds are added, 8 times the amount of distilled water is added, and the mixture is boiled for 30min. After filtering to remove impurities, the mixture is concentrated into a clear paste with a relative density of 1.15 (60°C), that is, old honey is made.

8. The ginseng product with antioxidant, anti-aging and longevity-prolonging effects obtained by the preparation process of the ginseng product with antioxidant, anti-aging and longevity-prolonging effects as claimed in any one of claims 2 to 7.

Images