Gel for treating animal mastitis and preparation method thereof

Abstract

The invention provides a gel for treating animal mastitis and a preparation method thereof, and relates to the technical field of medicines, the gel is prepared by taking dandelion, houttuynia cordata and wild chrysanthemum flower in a specific ratio as main raw materials and adopting a specific process. Specifically, effective components in dandelion are extracted by means of decoction, filtration, concentration, ethanol addition and the like, effective components in wild chrysanthemum flowers and heartleaf houttuynia herbs are extracted by a steam distillation method, and then the effective components are mixed with a humectant, propylene glycol, trichloro hydroxydiphenyl ether, a preservative, a pH regulator and water to obtain the gel. The gel prepared by the method disclosed by the invention can pass through a medicine patch of an ultrasonic medicine penetration instrument or can be directly smeared on a mammary inflammation area of an animal, the damage of gastrointestinal tract digestive juice to the medicine is reduced without the first-pass effect of the liver, and the gel has a better curative effect on treating the animal mastitis.

Description

Technical Field

[0001] The invention relates to the technical field of medicines, and in particular to a gel for treating animal mastitis and a preparation method thereof. Background Art

[0002] In the animal husbandry industry, mastitis, as a widespread disease, poses a serious threat to the benefits of farming. The disease not only causes a sharp drop in animal milk production, but also seriously weakens the overall production performance of animals, greatly reducing the quality of milk. The causes of mastitis are complex and diverse, among which infection by pathogens is one of the main causes. These pathogens may be hidden in the animal's body or widely distributed in the surrounding environment in which they live. When the environmental sanitation conditions of the animal are poor or its own immunity is reduced, the risk of infection by pathogens increases significantly.

[0003] Traditionally, the treatment of animal mastitis mainly relies on antibiotics. Although the use of antibiotics has alleviated the condition to a certain extent, the disadvantages of their long-term use are becoming increasingly prominent. On the one hand, the frequent use of antibiotics has aggravated the drug resistance of pathogenic microorganisms, making the originally effective drugs gradually ineffective; on the other hand, the problem of antibiotic residues in milk has become increasingly serious, far exceeding safety standards, posing a potential threat to human food safety.

[0004] In view of this, it has become a top priority to find new treatment methods to replace antibiotics. Among them, traditional Chinese medicine and other non-antibiotic therapies have attracted much attention due to their unique advantages. However, the current traditional Chinese medicine agents used to treat animal mastitis still have many inconveniences in the way they are administered. Oral administration and injection are the main routes of administration, which not only increases the complexity of the operation, but may also cause additional stress reactions to the animals. In addition, although traditional Chinese medicine has shown certain potential in the treatment of mastitis, its efficacy still needs to be further improved to meet the actual needs of clinical treatment. Summary of the invention

[0005] The problem solved by the invention is that the Chinese medicinal preparations for treating animal mastitis are generally taken orally and injected, which is not very convenient to use and the efficacy needs to be further improved.

[0006] In order to solve the above problems, the present invention provides a method for preparing a gel for treating animal mastitis, comprising:

[0007] Step S1, boiling dandelion with water to obtain a decoction; filtering the decoction, concentrating it to a thick paste, adding an ethanol solution to obtain a first mixture; allowing the first mixture to stand for a treatment, filtering it, removing ethanol from the obtained filtrate, and obtaining a second mixture; concentrating the second mixture to a thick paste to obtain a dandelion extract;

[0008] Step S2, extracting volatile oil from wild chrysanthemum and houttuynia cordata by steam distillation and collecting distillate to obtain volatile oil and distillate;

[0009] Step S3, adding carbomer to the distillate, mixing evenly, and then sequentially adding a moisturizer, propylene glycol, trichlorohydroxydiphenyl ether and a preservative, stirring evenly, and standing for a preset time to obtain a drug-loaded matrix;

[0010] Step S4, sequentially adding the dandelion extract and the volatile oil to the drug-carrying matrix, adding a pH adjuster to adjust the pH value to 7.0-7.5, adding water, stirring evenly, and obtaining a gel;

[0011] Among them, the amounts of raw materials used in the preparation process of the gel are as follows, by weight: 2000-3000 parts of dandelion, 2000-3000 parts of houttuynia, 2000-3000 parts of wild chrysanthemum, 6-12 parts of carbomer, 400-600 parts of moisturizer, 400-600 parts of propylene glycol, 40-60 parts of trichlorohydroxydiphenyl ether, and 20-30 parts of preservative.

[0012] Optionally, in step S1, the mass fraction of ethanol in the first mixture is 65-75%.

[0013] Optionally, in step S1, the static treatment time is 10-14 hours.

[0014] Optionally, in step S2, the ratio of the total mass of the dandelion, the wild chrysanthemum and the houttuynia cordata to the mass of the distillate is 1:(0.35-0.45).

[0015] Optionally, in step S3, the preset time is 3-5 hours.

[0016] Optionally, in step S3, the moisturizing agent is glycerin.

[0017] Optionally, in step S3, the preservative is sodium ethylparaben.

[0018] Optionally, in step S4, the pH adjuster is triethanolamine.

[0019] Optionally, in the step S4, the amount of the gelling agent is 11100-11300 parts by weight.

[0020] The present invention also provides a gel for treating animal mastitis, which is prepared by the above-mentioned preparation method of the gel for treating animal mastitis.

[0021] Compared with the prior art, the present invention uses dandelion, houttuynia cordata and wild chrysanthemum in a specific ratio as the main raw materials, and adopts a specific process to prepare a gel. Specifically, the effective ingredients in dandelion are extracted by means of decoction, filtration, concentration, and addition of ethanol, and the effective ingredients in wild chrysanthemum and houttuynia cordata are extracted by steam distillation, and then mixed with a moisturizer, propylene glycol, trichlorohydroxy diphenyl ether and a preservative, a pH regulator and water to obtain a gel. The dandelion extract in the gel has a bactericidal effect on Staphylococcus aureus, which is easy to cause animal mastitis, and has the effect of unblocking milk vascular obstruction to promote lactation. The extract of houttuynia cordata also has a good inhibitory effect on Staphylococcus aureus, and houttuynia cordata also has the effects of antiviral, improving body immunity, and promoting tissue regeneration. The wild chrysanthemum extract has the effect of inhibiting platelet aggregation and promoting leukocyte phagocytosis of Staphylococcus aureus. The moisturizer and propylene glycol help to improve the skin feel and stability of the gel, which is conducive to external application. The gel prepared by the method of the present invention can be applied to the breast inflammation area of ​​animals through the drug patch of an ultrasonic drug permeation instrument or directly, without the first-pass effect of the liver, reducing the damage of the gastrointestinal digestive juice to the drug. The gel has a good effect in treating animal mastitis. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 The present invention is a schematic diagram of a preparation method of a gel for treating animal mastitis according to an embodiment of the present invention. DETAILED DESCRIPTION

[0023] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the specific embodiments of the present invention are described in detail below in conjunction with the accompanying drawings. Although certain embodiments of the present invention are shown in the accompanying drawings, it should be understood that the present invention can be implemented in various forms and should not be interpreted as being limited to the embodiments described herein. On the contrary, these embodiments are provided to provide a more thorough and complete understanding of the present invention. It should be understood that the drawings and embodiments of the present invention are only for exemplary purposes and are not intended to limit the scope of protection of the present invention.

[0024] Unless otherwise defined, all technical and scientific terms used in this application have the same meanings as those commonly understood by those skilled in the art to which this application belongs; the terms used in the specification of this application are only for the purpose of describing specific implementation methods and are not intended to limit this application;

[0025] The term "including" and its variations used in this article are open inclusions, that is, "including but not limited to"; the term "based on" is "based at least in part on"; the term "one embodiment" means "at least one embodiment"; the term "another embodiment" means "at least one other embodiment"; the term "some embodiments" means "at least some embodiments"; the term "optionally" means "optional embodiments". The relevant definitions of other terms will be given in the following description. It should be noted that the concepts of "first" and "second" mentioned in the present invention are used to distinguish different objects, rather than to describe a specific order or a primary and secondary relationship. In addition, the terms "first" and "second" are only used for descriptive purposes and cannot be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Therefore, the features defined as "first" and "second" may explicitly or implicitly include one or more of the features. In the description of this application, unless otherwise specified, the meaning of "multiple" is two or more.

[0026] like Figure 1 As shown, a method for preparing a gel for treating animal mastitis provided by an embodiment of the present invention comprises:

[0027] Step S1, boiling dandelion with water to obtain a decoction; filtering the decoction, concentrating it to a thick paste, adding an ethanol solution to obtain a first mixture; allowing the first mixture to stand for a treatment, filtering it, removing ethanol from the obtained filtrate, and obtaining a second mixture; concentrating the second mixture to a thick paste to obtain a dandelion extract;

[0028] Step S2, extracting volatile oil from wild chrysanthemum and houttuynia cordata by steam distillation and collecting distillate to obtain volatile oil and distillate;

[0029] Step S3, adding carbomer to the distillate, mixing evenly, and then sequentially adding a moisturizer, propylene glycol, trichlorohydroxydiphenyl ether and a preservative, stirring evenly, and standing for a preset time to obtain a drug-loaded matrix;

[0030] Step S4, sequentially adding the dandelion extract and the volatile oil to the drug-carrying matrix, adding a pH adjuster to adjust the pH value to 7.0-7.5, adding water, stirring evenly, and obtaining a gel;

[0031] Among them, the amounts of raw materials used in the preparation process of the gel are as follows, by weight: 2000-3000 parts of dandelion, 2000-3000 parts of houttuynia, 2000-3000 parts of wild chrysanthemum, 6-12 parts of carbomer, 400-600 parts of moisturizer, 400-600 parts of propylene glycol, 40-60 parts of trichlorohydroxydiphenyl ether, and 20-30 parts of preservative.

[0032] The embodiment of the present invention uses dandelion, houttuynia cordata and wild chrysanthemum in a specific ratio as the main raw materials, and adopts a specific process to prepare a gel. Specifically, the effective ingredients in dandelion are extracted by means of decoction, filtration, concentration, and addition of ethanol, and the effective ingredients in wild chrysanthemum and houttuynia cordata are extracted by steam distillation, and then mixed with moisturizer, propylene glycol, trichlorohydroxy diphenyl ether and preservative, pH regulator and water to obtain a gel. The dandelion extract in the gel has a bactericidal effect on Staphylococcus aureus, which is easy to cause animal mastitis, and has the effect of dredging milk vascular obstruction to promote lactation. The extract of houttuynia cordata also has a good inhibitory effect on Staphylococcus aureus, and houttuynia cordata also has the effects of antiviral, improving body immunity, and promoting tissue regeneration. The wild chrysanthemum extract has the effect of inhibiting platelet aggregation and promoting leukocyte phagocytosis of Staphylococcus aureus. Moisturizer and propylene glycol help to improve the skin feel and stability of the gel, which is conducive to external application. The gel prepared by the method of the embodiment of the present invention can be applied to the inflamed area of ​​the breast of an animal through a drug patch of an ultrasonic drug permeation instrument or directly, without passing through the first-pass effect of the liver, thereby reducing the damage of the drug by the gastrointestinal digestive juice. The gel has a good effect in treating animal mastitis.

[0033] In some embodiments of the present invention, in step S1, the mass fraction of ethanol in the first mixture is 65-75%.

[0034] In some embodiments of the present invention, in step S1, the static treatment time is 10-14 hours.

[0035] In some embodiments of the present invention, in step S2, the ratio of the total mass of the dandelion, the wild chrysanthemum and the houttuynia cordata to the mass of the distillate is 1:(0.35-0.45).

[0036] In some embodiments of the present invention, in step S3, the preset time is 3-5 hours.

[0037] In some embodiments of the present invention, in step S3, the moisturizing agent is glycerin.

[0038] In some embodiments of the present invention, in step S3, the preservative is sodium ethylparaben.

[0039] In some embodiments of the present invention, in step S4, the pH adjuster is triethanolamine.

[0040] In some embodiments of the present invention, in the step S4, the amount of the gelling agent is 11100-11300 parts by weight.

[0041] The embodiment of the present invention further provides a gel for treating animal mastitis, which is prepared by the preparation method of the gel for treating animal mastitis as described above.

[0042] The present invention is further described below in conjunction with specific embodiments.

[0043] Example 1

[0044] A1. Add deionized water to 2240 g of dandelion and decoct twice, each decoction lasting 1 hour, and combine the decoctions to obtain a decoction; filter the decoction, concentrate it to a thick paste, add ethanol solution (mass fraction 95%) to obtain a first mixture; let the first mixture stand for 12 hours, filter it, remove ethanol from the obtained filtrate, and obtain a second mixture; concentrate the second mixture to a thick paste to obtain a dandelion extract; wherein the mass fraction of ethanol in the first mixture is 70%.

[0045] A2. Extract volatile oil from 2240 g of Houttuynia cordata and 2240 g of Chrysanthemum indici by steam distillation and collect distillate to obtain volatile oil and distillate; wherein the ratio of the total mass of the dandelion, the Chrysanthemum indici and the Houttuynia cordata to the mass of the distillate is 1:0.4.

[0046] A3. Add 8.4 g of carbomer (914 type) to the distillate, mix well, then add 560 g of glycerol, 560 g of propylene glycol, 44.8 g of trichlorohydroxydiphenyl ether and 22.4 g of sodium ethylparaben in sequence, stir well, and let stand for 4 hours to obtain a drug-loaded matrix.

[0047] A4. Add the dandelion extract and the volatile oil to the drug-carrying matrix in sequence, add triethanolamine as a pH adjuster, adjust the pH value to 7.0-7.5, add deionized water, stir evenly, and obtain a gel; the mass of the gel is 11200 g.

[0048] Example 2

[0049] A1. Add deionized water to 3000 g of dandelion and decoct twice, each decoction lasts for 1 hour, and combine the decoctions to obtain a decoction; filter the decoction, concentrate it to a thick paste, add ethanol solution (mass fraction 95%) to obtain a first mixture; let the first mixture stand for 12 hours, filter it, remove ethanol in the obtained filtrate, and obtain a second mixture; concentrate the second mixture to a thick paste to obtain a dandelion extract; wherein the mass fraction of ethanol in the first mixture is 70%.

[0050] A2. Extract volatile oil from 2500g of Houttuynia cordata and 2000g of Chrysanthemum indici by steam distillation and collect distillate to obtain volatile oil and distillate; wherein the ratio of the total mass of the dandelion, the Chrysanthemum indici and the Houttuynia cordata to the mass of the distillate is 1:0.4.

[0051] A3. Add 9 g of carbomer (type 914) to the distillate, mix well, then add 600 g of glycerol, 600 g of propylene glycol, 45.0 g of trichlorohydroxydiphenyl ether and 20.0 g of sodium ethylparaben in sequence, stir well, and let stand for 4 h to obtain a drug-loaded matrix.

[0052] A4. Add the dandelion extract and the volatile oil to the drug-carrying matrix in sequence, add triethanolamine as a pH adjuster, adjust the pH value to 7.0-7.5, add deionized water, stir evenly, and obtain a gel; the mass of the gel is 11200 g.

[0053] Example 3

[0054] A1. Add deionized water to 3000 g of dandelion and decoct twice, each decoction lasts for 1 hour, and combine the decoctions to obtain a decoction; filter the decoction, concentrate it to a thick paste, add ethanol solution (mass fraction 95%) to obtain a first mixture; let the first mixture stand for 12 hours, filter it, remove ethanol in the obtained filtrate, and obtain a second mixture; concentrate the second mixture to a thick paste to obtain a dandelion extract; wherein the mass fraction of ethanol in the first mixture is 70%.

[0055] A2. Extract volatile oil from 2800 g of Houttuynia cordata and 2000 g of Chrysanthemum indici by steam distillation and collect distillate to obtain volatile oil and distillate; wherein the ratio of the total mass of the dandelion, the Chrysanthemum indici and the Houttuynia cordata to the mass of the distillate is 1:0.4.

[0056] A3. Add 8 g of carbomer (914 type) to the distillate, mix well, then add 500 g of glycerol, 500 g of propylene glycol, 42.0 g of trichlorohydroxydiphenyl ether and 22.0 g of sodium ethylparaben in sequence, stir well, and let stand for 4 h to obtain a drug-loaded matrix.

[0057] A4. Add the dandelion extract and the volatile oil to the drug-carrying matrix in sequence, add triethanolamine as a pH adjuster, adjust the pH value to 7.0-7.5, add deionized water, stir evenly, and obtain a gel; the mass of the gel is 11200 g.

[0058] Comparative Example 1

[0059] A1. Add deionized water to 2240 g of Viola yedoensis and decoct twice, each decoction lasts for 1 hour, and combine the decoctions to obtain a decoction; filter the decoction, concentrate it to a thick paste, add ethanol solution (mass fraction 95%) to obtain a first mixture; let the first mixture stand for 12 hours, filter it, remove ethanol in the obtained filtrate, and obtain a second mixture; concentrate the second mixture to a thick paste to obtain a dandelion extract; wherein the mass fraction of ethanol in the first mixture is 70%.

[0060] A2. Extract volatile oil from 2240 g of Houttuynia cordata and 2240 g of Chrysanthemum indici by steam distillation and collect distillate to obtain volatile oil and distillate; wherein the ratio of the total mass of the Viola yedoensis, the Chrysanthemum indici and the Houttuynia cordata to the mass of the distillate is 1:0.4.

[0061] A3. Add 8.4 g of carbomer (914 type) to the distillate, mix well, then add 560 g of glycerol, 560 g of propylene glycol, 44.8 g of trichlorohydroxydiphenyl ether and 22.4 g of sodium ethylparaben in sequence, stir well, and let stand for 4 hours to obtain a drug-loaded matrix.

[0062] A4. Add the dandelion extract and the volatile oil to the drug-carrying matrix in sequence, add triethanolamine as a pH adjuster, adjust the pH value to 7.0-7.5, add deionized water, stir evenly, and obtain a gel; the mass of the gel is 11200 g.

[0063] Comparative Example 2

[0064] A1. Add deionized water to 2240 g of dandelion and decoct twice, each decoction lasting 1 hour, and combine the decoctions to obtain a decoction; filter the decoction, concentrate it to a thick paste, add ethanol solution (mass fraction 95%) to obtain a first mixture; let the first mixture stand for 12 hours, filter it, remove ethanol from the obtained filtrate, and obtain a second mixture; concentrate the second mixture to a thick paste to obtain a dandelion extract; wherein the mass fraction of ethanol in the first mixture is 70%.

[0065] A2. Extract volatile oil from 2240 g of honeysuckle and 2240 g of wild chrysanthemum by steam distillation and collect distillate to obtain volatile oil and distillate; wherein the ratio of the total mass of the dandelion, the wild chrysanthemum and the honeysuckle to the mass of the distillate is 1:0.4.

[0066] A3. Add 8.4 g of carbomer (914 type) to the distillate, mix well, then add 560 g of glycerol, 560 g of propylene glycol, 44.8 g of trichlorohydroxydiphenyl ether and 22.4 g of sodium ethylparaben in sequence, stir well, and let stand for 4 hours to obtain a drug-loaded matrix.

[0067] A4. Add the dandelion extract and the volatile oil to the drug-carrying matrix in sequence, add triethanolamine as a pH adjuster, adjust the pH value to 7.0-7.5, add deionized water, stir evenly, and obtain a gel; the mass of the gel is 11200 g.

[0068] Comparative Example 3

[0069] A1. Add deionized water to 2240 g of dandelion and decoct twice, each decoction lasting 1 hour, and combine the decoctions to obtain a decoction; filter the decoction, concentrate it to a thick paste, add ethanol solution (mass fraction 95%) to obtain a first mixture; let the first mixture stand for 12 hours, filter it, remove ethanol from the obtained filtrate, and obtain a second mixture; concentrate the second mixture to a thick paste to obtain a dandelion extract; wherein the mass fraction of ethanol in the first mixture is 70%.

[0070] A2. Extract volatile oil from 2240 g of Houttuynia cordata and 2240 g of Honeysuckle by steam distillation and collect distillate to obtain volatile oil and distillate; wherein the ratio of the total mass of the dandelion, the honeysuckle and the Houttuynia cordata to the mass of the distillate is 1:0.4.

[0071] A3. Add 8.4 g of carbomer (914 type) to the distillate, mix well, then add 560 g of glycerol, 560 g of propylene glycol, 44.8 g of trichlorohydroxydiphenyl ether and 22.4 g of sodium ethylparaben in sequence, stir well, and let stand for 4 hours to obtain a drug-loaded matrix.

[0072] A4. Add the dandelion extract and the volatile oil to the drug-carrying matrix in sequence, add triethanolamine as a pH adjuster, adjust the pH value to 7.0-7.5, add deionized water, stir evenly, and obtain a gel; the mass of the gel is 11200 g.

[0073] Experimental example

[0074] 1. In vitro antibacterial test

[0075] Preparation of test bacterial suspension: Staphylococcus aureus was streaked into mannitol sodium chloride agar medium and cultured at 37°C for 24 h; a single colony with a smooth surface and good growth was selected and inoculated into tryptone soy broth for pure culture, and then cultured in a constant temperature shaker (180 r / min) at 37°C for 24 h; after determining the logarithmic growth phase of Staphylococcus aureus by plate counting method, the number of viable bacteria in the bacterial solution in the logarithmic growth phase was determined, and the counted bacterial solution was adjusted to a bacterial solution concentration of 3.0×10 8 CFU / mL of Staphylococcus aureus suspension for later use.

[0076] Experimental Group 1

[0077] Take 50 μL of the test bacterial suspension and evenly spread it on the mannitol sodium chloride agar medium, and use a sterile puncher with a diameter of 4 mm to punch holes on each mannitol sodium chloride agar medium, and then add 50 μL of gel to each hole. The mannitol sodium chloride agar medium treated as above is placed in an electric constant temperature incubator, and after incubation at 37°C for 24 hours, the size of the inhibition zone is measured using a vernier caliper; wherein the gel used is the gel prepared in Example 1.

[0078] Experimental Group 2

[0079] The difference from Experimental Group 1 is that the gelling agent used is the gelling agent prepared in Example 2.

[0080] Experimental Group 3

[0081] The difference from Experimental Group 1 is that the gelling agent used is the gelling agent prepared in Example 3.

[0082] Control group 1

[0083] The difference from Experimental Group 1 is that the gelling agent used is the gelling agent prepared in Comparative Example 1.

[0084] Control group 2

[0085] The difference from Experimental Group 1 is that the gelling agent used is the gelling agent prepared in Comparative Example 2.

[0086] Control group 3

[0087] The difference from Experimental Group 1 is that the gelling agent used is the gelling agent prepared in Comparative Example 3.

[0088] The test results of the experimental groups 1-3 and the control groups 1-3 are shown in Table 1. It can be seen from Table 1 that the gels used in the experimental groups 1-3 and the control groups 1-3 have different degrees of antibacterial effects on Staphylococcus aureus, among which Staphylococcus aureus is extremely sensitive to the gel in Example 1, highly sensitive to the gel in Examples 2-3, and moderately sensitive to the gel in Comparative Examples 1-3, indicating that the gel in Examples 1-3 has a better antibacterial effect on Staphylococcus aureus.

[0089] Table 1

[0090] Group Diameter of inhibition zone (mm) Antibacterial level Experimental Group 1 21.54±0.17 ++++ Experimental Group 2 18.76±0.29 +++ Experimental Group 3 19.17±0.21 +++ Control group 1 14.62±0.26 ++ Control group 2 13.72±0.38 ++ Control group 3 12.95±0.23 ++

[0091] It should be noted that the inhibition zone determination criteria are: inhibition zone diameter > 20 mm, extremely sensitive (++++); inhibition zone diameter 15-20 mm, highly sensitive (+++); inhibition zone diameter 10-15 mm, moderately sensitive (++); inhibition zone diameter < 10 mm, low sensitivity (+); no inhibition zone, insensitive (-).

[0092] 2. Determination of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC)

[0093] Experimental Group 1

[0094] Take 10 sterile test tubes and number them 1-10 in sequence. After adding 3 mL of tryptone soy broth to test tubes 1-10 respectively, add 3 mL of gel to test tube 1, shake and mix, add the gel in test tube 1 to test tube 2, and so on, until the gel is taken out from test tube 8, so that the gel concentrations of test tubes 1-8 are 500 mg / mL, 250 mg / mL, 125 mg / mL, 62.50 mg / mL, 31.25 mg / mL, 15.63 mg / mL, 7.81 mg / mL and 3.91 mg / mL respectively; test tube 9 is the positive control, and test tube 10 is the blank control (no inoculation treatment); add 10 μL of liquid bacterial solution with a concentration of 3.0×10 8 After suspending Staphylococcus aureus with a concentration of CFU / mL, culture it in a constant temperature shaker (180r / min) at 37°C for 24 hours, and observe the turbidity changes in the test tube. The minimum drug concentration at which no turbidity changes occur is taken as the MIC value of the gel for Staphylococcus aureus. Based on the measured MIC value, 50 μL of liquid is taken from the test tubes where no turbidity changes occur and inoculated on mannitol sodium chloride agar medium, cultured at 37°C for 24 hours, and the results are observed. The minimum drug concentration corresponding to a colony count of less than 5 is taken as the MBC value of the gel for Staphylococcus aureus; wherein, the gel used is the gel prepared in Example 1.

[0095] Experimental Group 2

[0096] The difference from Experimental Group 1 is that the gelling agent used is the gelling agent prepared in Example 2.

[0097] Experimental Group 3

[0098] The difference from Experimental Group 1 is that the gelling agent used is the gelling agent prepared in Example 3.

[0099] Control group 1

[0100] The difference from Experimental Group 1 is that the gelling agent used is the gelling agent prepared in Comparative Example 1.

[0101] Control group 2

[0102] The difference from Experimental Group 1 is that the gelling agent used is the gelling agent prepared in Comparative Example 2.

[0103] Control group 3

[0104] The difference from Experimental Group 1 is that the gelling agent used is the gelling agent prepared in Comparative Example 3.

[0105] The test results of experimental groups 1-3 and control groups 1-3 are shown in Table 2. It can be seen from Table 2 that the MIC value and MBC value of experimental group 1 against Staphylococcus aureus are 7.81 mg / mL and 15.63 mg / mL respectively; the MIC value and MBC value of experimental group 2 and experimental group 3 against Staphylococcus aureus are 15.63 mg / mL and 31.25 mg / mL respectively; the MIC value and MBC value of control group 2 and control group 3 against Staphylococcus aureus are 31.25 mg / mL and 62.50 mg / mL respectively; the MIC value and MBC value of control group 1 against Staphylococcus aureus are 62.50 mg / mL and 125 mg / mL respectively. The above results show that experimental group 1 has the best antibacterial effect against Staphylococcus aureus.

[0106] Table 2

[0107]

[0108]

[0109] 3. Experiment on the efficacy of treating mastitis in dairy cows

[0110] Experimental Group 1

[0111] Twenty 5-year-old cows with clinical bovine mastitis, 3-4 litters, were selected from a dairy farm in Anda City, Heilongjiang Province. Ultrasonic transdermal administration was performed on the base of the inflamed nipple area of ​​each cow once a day for 5 consecutive days, and the number of cured cows was counted; wherein the drug used for ultrasonic transdermal administration was the gel prepared in Example 1.

[0112] Experimental Group 2

[0113] The difference from Experimental Group 1 is that the gelling agent used is the gelling agent prepared in Example 2.

[0114] Experimental Group 3

[0115] The difference from Experimental Group 1 is that the gelling agent used is the gelling agent prepared in Example 3.

[0116] Control group 1

[0117] The difference from Experimental Group 1 is that the gelling agent used is the gelling agent prepared in Comparative Example 1.

[0118] Control group 2

[0119] The difference from Experimental Group 1 is that the gelling agent used is the gelling agent prepared in Comparative Example 2.

[0120] Control group 3

[0121] The difference from Experimental Group 1 is that the gelling agent used is the gelling agent prepared in Comparative Example 3.

[0122] The statistical results of the experimental groups 1-3 and the control groups 1-3 are shown in Table 3. It can be seen from Table 3 that the gel prepared in Examples 1-3 has a better therapeutic effect on bovine mastitis.

[0123] Table 3

[0124]

[0125]

[0126] Although the present invention is disclosed as above, the protection scope of the present invention is not limited thereto. Those skilled in the art may make various changes and modifications without departing from the spirit and scope of the present invention, and these changes and modifications will fall within the protection scope of the present invention.

Claims

1. A method for preparing a gel for treating animal mastitis, characterized in that: include: Step S1, boiling dandelion with water to obtain a decoction; The decoction is filtered and concentrated to a thick paste, and an ethanol solution is added to obtain a first mixture; After the first mixture is allowed to stand for treatment, it is filtered to remove ethanol from the obtained filtrate to obtain a second mixture; the second mixture is concentrated into a thick paste to obtain a dandelion extract; Step S2, extracting volatile oil from wild chrysanthemum and houttuynia cordata by steam distillation and collecting distillate to obtain volatile oil and distillate; Step S3, adding carbomer to the distillate, mixing evenly, and then sequentially adding a moisturizer, propylene glycol, trichlorohydroxydiphenyl ether and a preservative, stirring evenly, and standing for a preset time to obtain a drug-loaded matrix; Step S4, sequentially adding the dandelion extract and the volatile oil to the drug-carrying matrix, adding a pH adjuster to adjust the pH value to 7.0-7.5, adding water, stirring evenly, and obtaining a gel; Among them, the amounts of raw materials used in the preparation process of the gel are as follows, by weight: 2000-3000 parts of dandelion, 2000-3000 parts of houttuynia, 2000-3000 parts of wild chrysanthemum, 6-12 parts of carbomer, 400-600 parts of moisturizer, 400-600 parts of propylene glycol, 40-60 parts of trichlorohydroxydiphenyl ether, and 20-30 parts of preservative.

2. The method for preparing a gel for treating animal mastitis according to claim 1, characterized in that: In the step S1, the mass fraction of ethanol in the first mixture is 65-75%.

3. The method for preparing a gel for treating animal mastitis according to claim 1, characterized in that: In the step S1, the static treatment time is 10-14 hours.

4. The method for preparing the gel for treating animal mastitis according to claim 1, characterized in that: In the step S2, the ratio of the total mass of the dandelion, the wild chrysanthemum and the houttuynia cordata to the mass of the distillate is 1:(0.35-0.45).

5. The method for preparing the gel for treating animal mastitis according to claim 1, characterized in that: In step S3, the preset time is 3-5 hours.

6. The method for preparing the gel for treating animal mastitis according to claim 1, characterized in that: In the step S3, the moisturizing agent is glycerin.

7. The method for preparing the gel for treating animal mastitis according to claim 1, characterized in that: In the step S3, the preservative is sodium ethylparaben.

8. The method for preparing the gel for treating animal mastitis according to claim 1, characterized in that: In the step S4, the pH adjuster is triethanolamine.

9. The method for preparing the gel for treating animal mastitis according to claim 1, characterized in that: In terms of weight, in step S4, the amount of the gelling agent is 11100-11300 parts.

10. A gel for treating animal mastitis, characterized in that: The gel for treating animal mastitis is prepared by the preparation method of any one of claims 1 to 9.

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