Traditional Chinese medicinal material micromolecule extraction preparation for tonifying yang and preparation method of traditional Chinese medicinal material micromolecule extraction preparation

Abstract

The invention belongs to the technical field of traditional Chinese medicine, and particularly relates to a traditional Chinese medicine micromolecule extraction preparation for tonifying yang and a preparation method thereof. The preparation method comprises the following steps: soaking raw materials in yellow wine, and then crushing and mixing the soaked raw materials to obtain a first mixture; soaking fructus lycii, ginseng and prepared rehmannia root in yellow rice wine, and boiling with water; soaking the primarily treated penis cervi in the boiling liquid; adding the cooled boiling water into the first mixture twice; adding acetic acid with a matched mass into the second mixed slurry, and then adding a micro-ecological strain for micro-ecological fermentation; carrying out ultrasonic extraction to obtain an extracting solution, refining, drying and curing. The acetic acid is adopted to adjust the PH value of the mixture, and the medicinal materials do not meet high temperature in the whole process, so that the activity of the fermented mixed strain is ensured, the activity of each medicinal material is also ensured, and the conversion and release of active ingredients in each medicinal material such as penis et testis cervi and tail cervi are promoted.

Description

Technical Field

[0001] The invention belongs to the technical field of traditional Chinese medicine, and specifically relates to a small molecule extraction preparation of Chinese medicinal materials for tonifying yang and a preparation method thereof. Background Art

[0002] As an important part of traditional Chinese culture, Chinese medicine has a long history and wide application in health care and disease treatment. With the deepening of Chinese medicine research, more and more Chinese medicinal materials have been found to have the function of regulating human balance and promoting health. With the increasing life and work pressure of young and middle-aged people in society, many people are in sub-healthy state. One of the more obvious sub-healthy conditions is yang deficiency. The continuous yang deficiency sub-healthy state makes the human body's immunity decline and physical fitness weak, which is easy to cause forgetfulness, difficulty falling asleep, dreaming and sweating, fatigue, back pain, cold hands and feet and other symptoms.

[0003] Therefore, it is necessary to carry out timely yang-tonifying and conditioning for people with yang deficiency. The existing products for tonifying yang and benefiting the kidney are mainly traditional Chinese medicine and health care products. There are generally inaccurate types and dosages of medicines, resulting in poor conditioning effects and high costs. At the same time, the use of traditional Chinese medicine or health care drugs makes users have poor appetite, and the food cannot keep up, affecting the conditioning effect of the users. Summary of the invention

[0004] The purpose of the present invention is to overcome the defects of the prior art in that the types and dosages of drugs used are not accurate, resulting in poor conditioning effects, and to provide a small molecule extract preparation of Chinese herbal medicine with good yang-tonifying effect and a preparation method thereof.

[0005] The technical solution adopted by the present invention to solve its technical problem is:

[0006] As a first aspect, a method for preparing a small molecule extract preparation of a Chinese herbal medicine for tonifying yang comprises the following steps:

[0007] Step 1: Weigh cloves, deer tails, oysters, chicken gizzard lining, hawthorn, poria, walnut kernels, and turtle shells of matching mass, wash and dry the raw materials, soak them in rice wine for 1-2 hours, and then crush and mix the soaked raw materials to obtain a first mixed material;

[0008] Step 2: Weigh matching amounts of ginseng, wolfberry and processed rehmannia root, soak the wolfberry, ginseng and processed rehmannia root in rice wine for 2-3 hours, add water and boil for 2-3 hours, add matching amounts of sodium chloride after boiling to obtain boiling liquid, and crush the boiled ginseng, wolfberry and processed rehmannia root into the boiling liquid, cool and set aside;

[0009] Step 3: weigh deer penis of matching mass, perform preliminary treatment on the deer penis, and soak the deer penis after preliminary treatment in the boiling liquid obtained in step 2 for 4-5 hours;

[0010] Step 4: Add the boiled water cooled in step 2 twice to the first mixture in step 1, stir evenly at a temperature of 27-44 degrees to obtain a uniformly distributed second mixture;

[0011] Step 5: first add acetic acid of matching mass to the second mixed slurry, adjust the second mixed slurry to weak acidity, and then add microecological bacteria for microecological fermentation; the fermentation temperature is 27-37 degrees, and the fermentation time is 20-25 days;

[0012] Step 6: After the fermentation is completed, ultrasonic extraction is used to obtain the extract;

[0013] Step 7: Refining treatment to obtain a pure small molecule extract, that is, using high performance liquid chromatography or supercritical fluid extraction separation technology to remove impurities and residual solvents from the extract obtained in step 5 to obtain a pure small molecule extract;

[0014] Step 8: Drying and solidification treatment to obtain the final Chinese herbal medicine solid particle extraction preparation.

[0015] Specifically, the initial treatment of the deer penis in step 3 includes:

[0016] The obtained deer penis is burned to remove the hair;

[0017] Use yellow wine for cleaning;

[0018] Slice the deer whip after washing it with rice wine.

[0019] Specifically, the pH value of the weakly acidic second mixed slurry in step 5 is 5.5-6.45.

[0020] Specifically, the microecological strains in step five are mixed strains of lactic acid bacteria and intestinal microecological flora; the intestinal microecological flora includes at least one of Bacteroides, Clostridium, and Bifidobacterium.

[0021] Specifically, the volume proportion of lactic acid bacteria in the mixed bacteria is 20-60%.

[0022] Specifically, in step eight, the drying and curing process includes the following contents:

[0023] The extract is air-dried in an air dryer, that is, the initial air-drying temperature is 27°C to 31°C, the final temperature is controlled at 33°C to 36°C, the pressure is maintained at 0.5Pa to 1.0Pa, and the air-drying time is adjusted according to the amount of material until the water is completely sublimated to obtain a block extract;

[0024] The block extract is cut into a solid particle extract preparation of Chinese medicinal materials.

[0025] As a second aspect, a small molecule extract preparation of traditional Chinese medicine for tonifying yang includes the following components in percentage by mass: 0.2-5 parts of cloves, 15-30 parts of deer whip, 9-30 parts of deer tail, 2-10 parts of oyster, 0.5-1 parts of chicken gizzard lining, 1-2 parts of hawthorn, 5-20 parts of Poria cocos, 2-20 parts of wolfberry, 10-15 parts of walnut kernel, 2-5 parts of turtle shell, 0.5-1 parts of ginseng, 0.5-1 parts of processed Rehmannia root, and 0.2-2 parts of acetic acid.

[0026] Specifically, it also includes 1-2 parts of yam and 1-2 parts of cnidium monnieri.

[0027] Specifically, it also includes 1-3 parts of mulberries and 0.2-1 part of sodium chloride.

[0028] Specifically, the invention comprises 2 parts of cloves, 11 parts of deer whips, 11 parts of deer tails, 42 parts of oysters, 1 part of chicken gizzard lining, 2 parts of hawthorn, 10 parts of Poria cocos, 2 parts of wolfberry, 10 parts of walnut kernels, 3 parts of turtle shells, 1 part of yam, 1 part of cnidium monnieri, 1 part of ginseng, 1 part of mulberry, 0.6 part of processed Rehmannia root, 0.2 part of acetic acid and 0.2 part of sodium chloride.

[0029] The beneficial effects of the Chinese herbal medicine small molecule extraction preparation for tonifying yang and the preparation method thereof of the present invention are:

[0030] The Chinese medicinal material small molecule extraction preparation for tonifying yang prepared by the present invention not only adds deer whip, deer tail, turtle shell, clove, oyster and other Chinese medicinal materials with aphrodisiac properties, but also adds chicken gizzard lining and hawthorn to help invigorate the stomach and digestion, reduce the user's stomach discomfort, add ginseng to tonify the spleen and lungs and replenish vital energy, and each medicinal material is added in a specific proportion, so as to solve the problem that the Chinese medicine or health care medicine in the prior art has a poor effect on tonifying yang and regulating the human body and affects the appetite.

[0031] In the preparation process of the Chinese medicinal material small molecule extraction preparation for tonifying yang of the present invention, it is only necessary to boil ginseng, wolfberry and nine-processed rehmannia root soaked in rice wine, and other medicinal materials only need to be washed, soaked in rice wine, crushed and directly added into cold boiled water, and the medicinal liquid is extracted by fermentation process, and then the medicinal liquid is air-dried by an air dryer. In the whole preparation method, acetic acid is used to soften the medicinal materials, which are easy to be absorbed by the human body, and acetic acid is used to adjust the pH value of the mixture to ensure the activity of microecological strains. The medicinal materials are not exposed to high temperature throughout the whole process, which not only ensures the activity of the fermented mixed strains, but also ensures the activity of each medicinal material, promotes the transformation and release of active ingredients in each medicinal material such as deer whip and deer tail, so that the active small molecule content in the extraction preparation is higher and the variety is richer.

[0032] The activity of traditional Chinese medicine has always been an obstacle that the traditional Chinese medicine extract preparations in the prior art cannot overcome. The small molecule extract preparation of traditional Chinese medicine for tonifying yang prepared by the preparation method of the present application has a high content of active small molecules, rich variety and is easily absorbed by the human body. It has significant medicinal effects in tonifying the kidney and yang, strengthening the tendons and bones, and nourishing the body, and has greatly improved the medicinal effects of existing preparations. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] The present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0034] Figure 1 It is a flow chart of the preparation method of the small molecule extraction preparation of traditional Chinese medicine for tonifying yang in Example 1 of the present invention. DETAILED DESCRIPTION

[0035] The present invention will now be described in further detail with reference to the accompanying drawings. These drawings are simplified schematic diagrams, which only illustrate the basic structure of the present invention in a schematic manner, and therefore only show the components related to the present invention.

[0036] like Figure 1 The specific embodiment of the small molecule extract preparation of traditional Chinese medicine for tonifying yang of the present invention shown in the figure includes the following components in percentage by mass: 0.2-5 parts of cloves, 15-30 parts of deer whips, 9-30 parts of deer tails, 2-10 parts of oysters, 0.5-1 parts of chicken gizzard lining, 1-2 parts of hawthorn, 5-20 parts of Poria cocos, 2-20 parts of wolfberry, 10-15 parts of walnut kernels, 2-5 parts of turtle shells, 0.5-1 parts of ginseng, 0.5-1 parts of processed Rehmannia root, 0.2-2 parts of acetic acid, and 0.2-1 parts of sodium chloride. Among them, clove has the effects of warming the middle and relieving adverse effects, nourishing the kidney and assisting yang; deer whip has the effects of nourishing kidney essence, strengthening kidney yang, and strengthening the waist and knees; deer tail has the effects of warming the waist and knees and benefiting kidney essence; oyster has the effects of calming the nerves, subduing yang and nourishing yin, softening and dispersing nodules; chicken's gizzard has the effects of astringing essence and stopping nocturnal emission, relieving stranguria and dissolving stones, and digesting food and relieving stagnation; hawthorn has the effects of invigorating the stomach and digesting food, promoting qi and dispersing blood stasis, clearing turbidity and lowering fat; Poria has the effects of promoting diuresis and removing dampness, strengthening the spleen, and calming the mind; wolfberry has the effects of nourishing the liver and kidney, improving essence and improving eyesight; walnut kernel has the effects of nourishing the kidney , astringing semen, nourishing blood, moistening the intestines and promoting bowel movements, and quenching thirst; soft-shelled turtle shell has the effects of clearing away heat and calming wind, nourishing the liver, and replenishing calcium and blood; ginseng has the effects of greatly replenishing vital energy, nourishing the spleen and lungs, promoting fluid production and quenching thirst, calming the nerves and improving intelligence; nine-processed Rehmannia has the effects of nourishing the kidney and yin, nourishing blood and regulating menstruation, nourishing blood and replenishing deficiency; acetic acid is white vinegar, which is used here to soften chicken gizzard lining, turtle shell and other medicinal materials, which is beneficial for human absorption; sodium chloride here is neutral edible salt, and the addition of edible salt stimulates the secretion of saliva and gastric juice, which helps the body absorb the drug.

[0037] The small molecule extract preparation of traditional Chinese medicine used for tonifying yang also includes 1-2 parts of yam, 1-2 parts of cnidium monnieri, 1-2 parts of mulberry, and 0.2-1 part of sodium chloride. Specifically, yam has the effects of invigorating qi and nourishing yin, tonifying the spleen, lungs and kidneys, and astringing semen and stopping leukorrhea; cnidium monnieri has the effects of drying dampness and dispelling wind, warming the kidney and strengthening yang; mulberry has the effects of nourishing blood and nourishing yin, and promoting body fluid and quenching thirst.

[0038] The method for preparing the above-mentioned Chinese herbal medicine small molecule extract preparation for tonifying yang of the present invention comprises the following steps:

[0039] Step 1: Weigh cloves, deer tails, oysters, chicken gizzard lining, hawthorn, poria, walnut kernels, and turtle shells of matching mass, wash and dry the raw materials, soak them in rice wine for 1-2 hours, and then crush and mix the soaked raw materials to obtain a first mixed material;

[0040] Step 2: Weigh matching amounts of ginseng, wolfberry and processed rehmannia root, soak the wolfberry, ginseng and processed rehmannia root in rice wine for 2-3 hours, add water and boil for 2-3 hours, add matching amounts of sodium chloride after boiling to obtain boiling liquid, and crush the boiled ginseng, wolfberry and processed rehmannia root into the boiling liquid, cool and set aside;

[0041] Step 3: weigh deer penis of matching mass, perform preliminary treatment on the deer penis, and soak the deer penis after preliminary treatment in the boiling liquid obtained in step 2 for 4-5 hours;

[0042] Step 4: Add the boiled water cooled in step 2 twice to the first mixture in step 1, stir evenly at a temperature of 27-44 degrees to obtain a uniformly distributed second mixture;

[0043] Step 5: first add acetic acid of matching mass to the second mixed slurry, adjust the second mixed slurry to weak acidity, and then add microecological bacteria for microecological fermentation; the fermentation temperature is 27-37 degrees, and the fermentation time is 20-25 days;

[0044] Step 6: After the fermentation is completed, ultrasonic extraction is used to obtain the extract;

[0045] Step 7: Refining treatment to obtain a pure small molecule extract, that is, using high performance liquid chromatography or supercritical fluid extraction separation technology to remove impurities and residual solvents from the extract obtained in step 5 to obtain a pure small molecule extract;

[0046] Step 8: Drying and solidification treatment to obtain the final Chinese herbal medicine solid particle extraction preparation.

[0047] Embodiment 1

[0048] The small molecule extract preparation of Chinese medicinal materials for tonifying yang in the first embodiment includes, by weight percentage: 5 parts of cloves, 16 parts of deer whips, 30 parts of deer tails, 10 parts of oysters, 0.5 parts of chicken gizzard lining, 1 part of hawthorn, 7 parts of Poria cocos, 8 parts of wolfberry, 15 parts of walnut kernels, 5 parts of turtle shells, 0.5 parts of ginseng, 0.5 parts of processed Rehmannia root, 0.7 parts of acetic acid, and 0.8 parts of sodium chloride.

[0049] The detailed preparation process is described as follows:

[0050] In the first embodiment, a total of 100 g was used. 5 g of cloves, 30 g of deer tail, 10 g of oyster, 0.5 g of chicken gizzard lining, 1 g of hawthorn, 7 g of Poria, 15 g of walnut kernel, and 5 g of turtle shell raw materials of corresponding mass were weighed, and the raw materials were washed and dried, and then soaked in yellow wine for 1 hour, and then the soaked raw materials were crushed and mixed to obtain the first mixed material. 0.5 g of ginseng, 8 g of wolfberry, 0.5 g of nine-processed rehmannia and 0.8 g of sodium chloride were weighed, and wolfberry, ginseng and nine-processed rehmannia were soaked in yellow wine for 2 hours, and then water was added to boil for 90 minutes. After boiling, sodium chloride was added to obtain a boiled liquid, and the boiled ginseng, wolfberry and nine-processed rehmannia were crushed and blended in the boiled liquid, and cooled for use. 16g of deer whip is weighed and preliminarily treated. The preliminarily treated deer whip is placed in the boiling liquid obtained in step 2 and soaked for 5 hours. In this embodiment 1, the preliminarily treated deer whip includes burning the obtained deer whip to remove the hair; washing with yellow wine; and slicing the deer whip washed with yellow wine. Here, wolfberry, ginseng and nine-processed rehmannia are first soaked with yellow wine, so that the yellow wine reacts with the active molecules in wolfberry, ginseng and nine-processed rehmannia to increase the activity rate of the active molecules, and then the wolfberry, ginseng and nine-processed rehmannia soaked with yellow wine are boiled with water to ensure that the active molecules in wolfberry, ginseng and nine-processed rehmannia are still partially alive during the boiling process.

[0051] Then, the cooled boiled liquid is added to the first mixture twice, stirred evenly at 30 degrees to obtain a uniformly distributed second mixture, and microecological strains are added to the second mixed slurry for microecological fermentation, wherein the fermentation temperature is 30 degrees and the fermentation time is 20 days. It should be further explained here that 25% of the cooled boiled liquid is added to the first mixture for the first time to completely soak the first mixture, and the remaining boiled liquid is added to the first mixture for the second time to ensure the uniformity of mixing.

[0052] The microecological strains are mixed strains of lactic acid bacteria and intestinal microecological flora, wherein the intestinal microecological flora includes at least one of Bacteroides, Clostridium, and Bifidobacterium. Specifically, in this embodiment 1, the volume ratio of Bacteroides, Clostridium, and Bifidobacterium is 1:2:2, and the volume of lactic acid bacteria in the mixed strains accounts for 20-60%, and the lactic acid bacteria in this embodiment 1 accounts for 40%.

[0053] Because the microecological flora cannot survive in high temperature and particularly acidic environments, therefore, before fermentation, the pH value of the second mixed slurry is adjusted by acetic acid, and the pH value is 5.5-6.45, and the pH value of the second mixed slurry in the first embodiment is 6. After the fermentation is completed, ultrasonic extraction is used to obtain the extract, which is a relatively mature technology. It only needs to control the extraction temperature between 27 and 37 degrees. The ultrasonic extraction technology is not described in detail here. The obtained extract is treated with high-performance liquid chromatography or supercritical fluid extraction separation technology to remove impurities and residual solvents to obtain a pure small molecule extract. Similarly, the high-performance liquid chromatography or supercritical fluid extraction separation technology here is also used for a long time in the pharmaceutical process, and the high-performance liquid chromatography or supercritical fluid extraction separation technology is not described in detail.

[0054] Finally, the extract is dried and solidified, wherein the drying and solidification treatment includes the following: air-drying in an air dryer, that is, the initial air-drying temperature is 27°C to 31°C, the final temperature is controlled at 33°C to 36°C, the pressure is maintained at 0.5Pa to 1.0Pa, and the air-drying time is adjusted according to the amount of material until the water is completely sublimated to obtain a block extract; the block extract is cut to form a solid particle extraction preparation of Chinese herbal medicine. In this embodiment 1, the extract is air-dried in an air dryer, the initial air-drying temperature is 28°C, the final temperature is controlled at 35°C, the pressure is maintained at 0.5Pa, the air-drying time is 3 hours, the water is completely sublimated, a block extract is obtained, and the block extract is cut to form a solid particle extraction preparation of Chinese herbal medicine.

[0055] It should be emphasized that in order to explore the fastest and best fermentation conditions of microecological strains at suitable temperature and suitable pH value, the present invention tested the temperature, humidity and pH value of each active molecule. The conversion rate of active ingredients of the same strain at different temperatures and humidity is as follows:

[0056] Table 1:

[0057]

[0058] The conversion rate is calculated as follows:

[0059] Conversion rate = [(content after fermentation - content before fermentation) / (content before fermentation)] * 100%;

[0060] According to the above table and calculation formula, we can get:

[0061] Conversion rate after the first amino acid fermentation:

[0062] [(72.02-60.84) / 60.84]*100%=18.38%;

[0063] Conversion rate after the second amino acid fermentation:

[0064] [(78.56-60.84) / 60.84]*100%=29.12%;

[0065] Conversion rate of amino acids after the third fermentation:

[0066] [(82.22-60.84) / 60.84]*100%=35.14%;

[0067] Conversion rate after the fourth amino acid fermentation:

[0068] [(82.22-60.84) / 60.84]*100%=36.57%;

[0069] After a large number of experiments, in order to avoid high temperature destroying the survival of active ingredients, under the low temperature of 37°C, the fermentation temperature range was set to 27-37 degrees, the pH value was set to 5.5-6.4, and in the subsequent drying and curing process, freeze-drying technology was not used, but air-drying technology was used, and the initial air-drying temperature was 27°C to 31°C, and the final temperature was controlled at 33°C to 36°C, which can effectively ensure the effective conversion of the amino acid content in deer whips and deer tails. The efficient extraction and refining of active small molecules were achieved, and pure small molecule extracts were obtained, making the extracts easy for the human body to absorb and use. The provided Chinese herbal medicine microecological small molecule extracts have significant medicinal effects in tonifying the kidney and strengthening yang, strengthening tendons and bones, and nourishing the body.

[0070] Embodiment 2

[0071] The small molecule extract preparation of Chinese medicinal materials for tonifying yang in the second embodiment includes, by mass percentage: 2 parts of cloves, 30 parts of deer whips, 18 parts of deer tails, 10 parts of oysters, 1 part of chicken gizzard lining, 2 parts of hawthorn, 15 parts of Poria cocos, 2 parts of wolfberries, 10 parts of walnut kernels, 3 parts of turtle shells, 1 part of yam, 1 part of ginseng, 1 part of cnidium monnieri, 1 part of mulberry, 1 part of processed Rehmannia root, 1 part of acetic acid, and 1 part of sodium chloride.

[0072] The detailed preparation process is described as follows:

[0073] In the second embodiment, the total amount is 100g. Weigh 2g cloves, 18g deer tail, 10g oyster, 1g chicken gizzard lining, 2g hawthorn, 15g poria, 10g walnut kernel, and 3g turtle shell raw materials of corresponding mass, wash and dry the raw materials, soak them in yellow wine for 1 hour, and then crush and mix the soaked raw materials to obtain the first mixed material. Weigh 1g ginseng, 2g wolfberry, 1g nine-processed rehmannia root, and 1g sodium chloride. After soaking wolfberry, ginseng and nine-processed rehmannia root in yellow wine for 3 hours, add water and boil for 70 minutes. Add sodium chloride during boiling to obtain boiling liquid, and crush and fuse the boiled ginseng, wolfberry and nine-processed rehmannia root in the boiling liquid, and cool for use. Weigh 30g of deer whip, perform an initial treatment on the deer whip, soak the initially treated deer whip in the boiling liquid obtained in step 2 for 4 hours, then add the above-mentioned cooled boiling liquid to the first mixed material twice, stir evenly at a temperature of 28 degrees to obtain a uniformly distributed second mixed material, add microecological strains to the second mixed slurry for microecological fermentation, wherein the fermentation temperature is 27 degrees, and the fermentation time is 25 days. It should be further explained here that 25% of the cooled boiling liquid is added to the first mixed material for the first time to completely wet the first mixed material, and the remaining boiling liquid is added to the first mixed material for the second time to ensure the uniformity of mixing.

[0074] The microecological bacteria are a mixed strain of 60% lactic acid bacteria and 40% intestinal microecological bacteria. In the second embodiment, the volume ratio of Bacteroides, Clostridium and Bifidobacterium is 2:1:2.

[0075] The pH value of the second mixed slurry in this embodiment 1 is 6.43. After the fermentation is completed, ultrasonic extraction is used to obtain an extract, and the obtained extract is treated with high performance liquid chromatography to remove impurities and residual solvents to obtain a pure small molecule extract.

[0076] Finally, the extract is dried and solidified. In the second embodiment, the extract is air-dried in an air dryer. The initial air-drying temperature is 30°C, the final temperature is controlled at 34°C, the pressure is maintained at 1Pa, and the air-drying time is 2 hours. The water is completely sublimated to obtain a block extract, which is cut into a solid particle extract preparation of Chinese medicinal materials.

[0077] Embodiment 3

[0078] The small molecule extract preparation of Chinese medicinal materials for tonifying yang in the third embodiment includes, by mass percentage: 0.5 parts of cloves, 25 parts of deer whips, 22.5 parts of deer tails, 5 parts of oysters, 0.7 parts of chicken gizzard lining, 1.8 parts of hawthorn, 20 parts of Poria cocos, 1 part of wolfberry, 15 parts of walnut kernels, 3 parts of turtle shells, 2 parts of yam, 0.5 parts of ginseng, 1 part of cnidium monnieri, 1 part of mulberry, 0.5 parts of processed Rehmannia root, 0.3 parts of acetic acid, and 0.2 parts of sodium chloride.

[0079] The detailed preparation process is described as follows:

[0080] In the third embodiment, the total amount is 100g. Weigh the corresponding mass of 0.5g clove, 22.5g deer tail, 5g oyster, 0.7g chicken gizzard lining, 1.8g hawthorn, 20g Poria, 15g walnut kernel, 3g turtle shell, 2g yam, 1g cnidium monnieri, and 1g mulberry raw materials, wash and dry the raw materials, soak them in yellow wine for 1 hour, and then crush and mix the soaked raw materials to obtain the first mixed material. Weigh 1g ginseng, 2g wolfberry, 1g nine-processed rehmannia, and 0.2g sodium chloride. After soaking wolfberry, ginseng and nine-processed rehmannia in yellow wine for 2.5 hours, add water and boil for 110 minutes. Add sodium chloride during boiling to obtain boiling liquid, and crush and fuse the boiled ginseng, wolfberry and nine-processed rehmannia in the boiling liquid, and cool for use. Weigh 25g of deer whip, perform an initial treatment on the deer whip, soak the initially treated deer whip in the boiling liquid obtained in step 2 for 4 hours, then add the above-mentioned cooled boiling liquid to the first mixture twice, stir evenly at a temperature of 30 degrees to obtain a uniformly distributed second mixture, add microecological strains to the second mixed slurry for microecological fermentation, wherein the fermentation temperature is 30 degrees, and the fermentation time is 28 days. It should be further explained here that 25% of the cooled boiling liquid is added to the first mixture for the first time to completely wet the first mixture, and the remaining boiling liquid is added to the first mixture for the second time to ensure the uniformity of mixing.

[0081] The microecological bacteria are a mixed strain of 50% lactic acid bacteria and 50% intestinal microecological bacteria. In the second embodiment, the volume ratio of Bacteroides, Clostridium and Bifidobacterium is 1:1:1.

[0082] The pH value of the second mixed slurry in this embodiment 1 is 6.2. After the fermentation is completed, ultrasonic extraction is used to obtain an extract, and the obtained extract is treated with high performance liquid chromatography to remove impurities and residual solvents to obtain a pure small molecule extract.

[0083] Finally, the extract is dried and solidified. In the third embodiment, the extract is air-dried in an air dryer. The initial air-drying temperature is 29°C, the final temperature is controlled at 36°C, the pressure is maintained at 1Pa, and the air-drying time is 2.5 hours. The water is completely sublimated to obtain a block extract, which is cut into a solid particle extract preparation of Chinese medicinal materials.

[0084] Comparative Example 1

[0085] Comparative Example 1 is a Chinese medicine decoction obtained by boiling all the medicinal materials in Example 1 in an existing manner.

[0086] Comparative Example 2

[0087] Comparative Example 2 is a Chinese medicine decoction obtained by boiling all the medicinal materials in Example 2 in an existing manner.

[0088] Comparative Example 3

[0089] Comparative Example 3 is a Chinese medicine decoction obtained by boiling all the medicinal materials in Example 3 in an existing manner.

[0090] The solid particle extract preparations of Chinese medicinal materials obtained in Example 1, Example 2, and Example 3 and the Chinese medicinal decoctions obtained in Comparative Example 1, Comparative Example 2, and Comparative Example 3 were tested for active molecules. Specifically, the extract preparations obtained in Example 1, Example 2, and Example 3 were dissolved in 250 ml of water to obtain 250 ml of the Chinese medicinal decoctions boiled in Comparative Example 1, Comparative Example 2, and Comparative Example 3. The distribution coefficients of different active ingredients in the stationary phase and the mobile phase were used to separate them through a chromatographic column, and the quantification was performed through an ultraviolet-visible, fluorescence or mass spectrometry detector. The detection was performed using equipment that is relatively easy to obtain on the market. The comparative data table is as follows:

[0091] Table 2:

[0092] Detection of active peptide types Deer tail peptide (g / 100g) Poria polypeptide (g / 100g) Walnut peptide (g / 100g) Embodiment 1 8.3 3.8 4.3 Comparative Example 1 0.5 0.1 0 Embodiment 2 5.6 2.2 2.1 Comparative Example 2 0.2 0 0 Embodiment 3 7.1 5.4 4.3 Comparative Example 3 0.2 0 0.1

[0093] It can be clearly seen from Table 2 above that the survival rate of active peptides in the solid particle extraction preparation of Chinese herbal medicine prepared by the preparation method of the present invention is significantly higher than that of the active peptides in the Chinese herbal medicine decoction obtained by boiling in the prior art. In the preparation process of the small molecule extraction preparation of Chinese herbal medicine for tonifying Yang of the present invention, only ginseng, wolfberry and nine-processed Rehmannia root soaked in yellow wine need to be boiled with water, and other medicinal materials only need to be washed, soaked in yellow wine and crushed and then directly added to cold boiled water, and the medicinal liquid is extracted by fermentation process, and then the medicinal liquid is air-dried by air dryer. The whole preparation method softens the medicinal materials with acetic acid, which is easy for human body to absorb, and the pH value of the mixture is adjusted with acetic acid to ensure the activity of microecological strains. The medicinal materials are not subjected to high temperature throughout the process, which not only ensures the activity of the fermented mixed strains, but also ensures the activity of each medicinal material, promotes the transformation and release of active ingredients in each medicinal material such as deer whip and deer tail, so that the content of active small molecules in the extraction preparation is higher and the variety is richer.

[0094] A free clinic was conducted in an office building in Changzhou City. 30 people with yang deficiency and sub-health symptoms were selected and provided with the solid particle extract preparation of Chinese medicinal materials and Chinese medicinal decoction prepared by the present invention free of charge. Among them, 18 were male and 17 were female. They were divided into six groups, with 5 people in each group. A course of treatment for 6 days, twice a day, was conducted for the effect experiment. It should be noted that the solid particle extract preparation of Chinese medicinal materials of the present invention has passed the review of relevant departments and has an approval number or relevant certification information.

[0095] Six groups of people took the medicines of Example 1, Example 2, Example 3, Comparative Example 1, Comparative Example 2, and Comparative Example 3 orally, and collected feedback from the testers six days later. Among the people who took the solid particle extract of Chinese medicinal materials prepared by the first embodiment orally, two people reported that their fatigue was significantly reduced, and two people reported that the symptoms of cold hands and feet were relieved in the afternoon. Among the people who took the solid particle extract of Chinese medicinal materials prepared by the second embodiment orally, one person reported that the symptoms of dreaming and night sweats were completely improved, three people reported that their fatigue was significantly reduced, and two people reported that the symptoms of back pain were alleviated. Among the people who took the solid particle extract of Chinese medicinal materials prepared by the third embodiment orally, Three people reported that fatigue was significantly reduced, one person reported that his hands and feet felt hot after oral administration at night, and among the people who took Examples 1, 2 and 3 orally, three people had a loss of appetite, while among the 15 people who took the Chinese medicine decoctions of Comparative Documents 1, 2 and 3, a total of five people reported that fatigue was slightly reduced, but the reduction was not obvious, two people reported that the symptoms of cold hands and feet were slightly improved, and five people had a loss of appetite. It can be seen from the above experimental data that the yang-tonifying effect of the solid particle extraction preparation of Chinese medicinal materials with more active molecules prepared by the present invention is significantly higher than that of the Chinese medicinal decoctions boiled by the existing preparation method. And the loss of appetite of the Chinese medicinal materials solid particle extraction preparation prepared by the present invention is significantly better than that of the Chinese medicinal decoctions of the comparative example. It should be understood that whether it is the Chinese medicinal materials solid particle extraction preparation with more active molecules prepared by the invention, or the Chinese medicine costs of each Chinese medicine of the present application obtained by the prior art, the loss of appetite caused by Chinese medicine to personnel can be improved.

[0096] The Chinese herbal medicine small molecule extraction preparation for tonifying Yang prepared by the present invention, in addition to adding deer whip, deer tail, turtle shell, clove, oyster and other Chinese herbal medicine materials with aphrodisiac properties, is also added with chicken gizzard lining and hawthorn to help invigorate the stomach and digestion, reduce the discomfort of the user's stomach, add ginseng to tonify the spleen and lungs, and greatly replenish the vital energy, and each medicinal material is added in a specific ratio to solve the problem that the Chinese medicine or health medicine in the prior art has a poor effect on the tonifying Yang conditioning of the human body and affects the appetite. And the activity of Chinese medicine has always been an obstacle that the Chinese herbal medicine extraction preparation in the prior art cannot cross, and the Chinese herbal medicine small molecule extraction preparation for tonifying Yang prepared by the preparation method of the present application, due to the high content of active small molecules, rich variety and easy to be absorbed by the human body, has significant efficacy in tonifying the kidney and tonifying Yang, strengthening tendons and bones, and nourishing the body, and the efficacy of the existing preparation has been greatly improved.

[0097] It should be understood that the specific embodiments described above are only used to explain the present invention, and are not used to limit the present invention. Obvious changes or modifications derived from the spirit of the present invention are still within the protection scope of the present invention.

Claims

1. A method for preparing a small molecule extract preparation of Chinese herbal medicine for tonifying yang, characterized in that: The following steps are involved: Step 1: Weigh cloves, deer tails, oysters, chicken gizzard lining, hawthorn, poria, walnut kernels, and turtle shells of matching mass, wash and dry the raw materials, soak them in rice wine for 1-2 hours, and then crush and mix the soaked raw materials to obtain a first mixed material; Step 2: Weigh matching amounts of ginseng, wolfberry and processed rehmannia root, soak the wolfberry, ginseng and processed rehmannia root in rice wine for 2-3 hours, add water and boil for 2-3 hours, add matching amounts of sodium chloride after boiling to obtain boiling liquid, and crush the boiled ginseng, wolfberry and processed rehmannia root into the boiling liquid, cool and set aside; Step 3: weigh deer penis of matching mass, perform preliminary treatment on the deer penis, and soak the deer penis after preliminary treatment in the boiling liquid obtained in step 2 for 4-5 hours; Step 4: Add the boiled water cooled in step 2 twice to the first mixture in step 1, stir evenly at a temperature of 27-44 degrees to obtain a uniformly distributed second mixture; Step 5: first add acetic acid of matching mass to the second mixed slurry, adjust the second mixed slurry to weak acidity, and then add microecological bacteria for microecological fermentation; the fermentation temperature is 27-37 degrees, and the fermentation time is 20-25 days; Step 6: After the fermentation is completed, ultrasonic extraction is used to obtain the extract; Step 7: Refining treatment to obtain a pure small molecule extract, that is, using high performance liquid chromatography or supercritical fluid extraction separation technology to remove impurities and residual solvents from the extract obtained in step 5 to obtain a pure small molecule extract; Step 8: Drying and solidification treatment to obtain the final Chinese herbal medicine solid particle extraction preparation.

2. The method for preparing a small molecule extract preparation of Chinese herbal medicine for tonifying yang according to claim 1, characterized in that: The initial treatment of the deer penis in step 3 includes: The obtained deer penis is burned to remove the hair; Use yellow wine for cleaning; Slice the deer whip after washing it with rice wine.

3. The method for preparing a small molecule extract preparation of Chinese herbal medicine for tonifying yang according to claim 1, characterized in that: The pH value of the second weakly acidic mixed slurry in step 5 is 5.5-6.

45.

4. The method for preparing a small molecule extract preparation of Chinese herbal medicine for tonifying yang according to claim 1, characterized in that: The microecological strains in step 5 are mixed strains of lactic acid bacteria and intestinal microecological flora; the intestinal microecological flora includes at least one of Bacteroides, Clostridium, and Bifidobacterium.

5. The method for preparing a small molecule extract preparation of Chinese herbal medicine for tonifying yang according to claim 4, characterized in that: The volume proportion of lactic acid bacteria in the mixed bacteria is 20-60%.

6. The method for preparing a small molecule extract preparation of Chinese herbal medicine for tonifying yang according to claim 1, characterized in that: In step eight, the drying and curing process includes the following contents: The extract is air-dried in an air dryer, that is, the initial air-drying temperature is 27°C to 31°C, the final temperature is controlled at 33°C to 36°C, the pressure is maintained at 0.5Pa to 1.0Pa, and the air-drying time is adjusted according to the amount of material until the water is completely sublimated to obtain a block extract; The block extract is cut into a solid particle extract preparation of Chinese medicinal materials.

7. A small molecule extract preparation of Chinese herbal medicine for tonifying Yang, characterized in that: The small molecule extract preparation of traditional Chinese medicine for tonifying yang is prepared by the preparation method according to any one of claims 1 to 6, and includes the following components in percentage by mass: 0.2-5 parts of cloves, 15-30 parts of deer whips, 9-30 parts of deer tails, 2-10 parts of oysters, 0.5-1 parts of chicken gizzard lining, 1-2 parts of hawthorn, 5-20 parts of Poria cocos, 2-20 parts of wolfberry, 10-15 parts of walnut kernels, 2-5 parts of turtle shells, 0.5-1 parts of ginseng, 0.5-1 parts of processed Rehmannia root, 0.2-2 parts of acetic acid, and 0.2-1 parts of sodium chloride.

8. The Chinese herbal medicine small molecule extract preparation for tonifying yang according to claim 7, characterized in that: It also includes 1-2 portions of yam and 1-2 portions of cnidium monnieri.

9. The Chinese herbal medicine small molecule extract preparation for tonifying yang according to claim 8, characterized in that: Also includes 1-2 servings of mulberries.

10. The Chinese herbal medicine small molecule extract preparation for tonifying yang according to claim 9, characterized in that: The invention comprises 2 parts of cloves, 30 parts of deer whips, 18 parts of deer tails, 10 parts of oysters, 1 part of chicken gizzard lining, 2 parts of hawthorns, 15 parts of tuckahoe, 2 parts of wolfberries, 10 parts of walnut kernels, 3 parts of turtle shells, 1 part of yam, 1 part of cnidium monnieri, 1 part of ginseng, 1 part of mulberries, 1 part of processed rehmannia root, 1 part of acetic acid and 1 part of sodium chloride.

Images