Bacteriostatic spray and preparation method and application thereof

Abstract

The invention relates to the technical field of bacteriostatic spray, and provides bacteriostatic spray as well as a preparation method and application thereof. The bacteriostatic spray is prepared from the following raw materials: a rhodiola rosea extracting solution, a saffron crocus extracting solution, a lamiophlomis rotata extracting solution, a rhizoma drynariae extracting solution, an angelica sinensis extracting solution, a radix clematidis extracting solution, a ligusticum wallichii extracting solution, a garden balsam stem extracting solution, a lycopodium clavatum extracting solution, a radix salviae miltiorrhizae extracting solution, a cassia twig extracting solution, turpentine, borneol, trichloro hydroxydiphenyl ether, a lavender extracting solution and auxiliary liquid. According to the antibacterial spray disclosed by the invention, various natural active substance components can be fused, so that the antibacterial spray has the effects of inhibiting bacteria, preserving moisture, protecting skin, resisting inflammation and soothing the nerves, is rich in fresh, cool and fragrant and sweet smell, is mild to skin, is safe and non-irritant, and does not harm human skin, so that the antibacterial spray has more effects and a better use effect; various requirements of people can be met, and the product capacity is better.

Description

Technical Field

[0001] The invention relates to the technical field of antibacterial spray, in particular to an antibacterial spray. The invention also relates to a preparation method and application of the antibacterial spray. Background Art

[0002] At present, with the significant improvement of the public's awareness of sanitary environment and healthy disinfection, the demand for medical protection and disinfection products is continuing to grow, and the demand for disinfection products in various regions has risen sharply, which has also promoted the continuous expansion of the disinfection product market. Among them, the disinfection and antibacterial spray, which is indispensable in daily life, has become a must-have for people at home and on the go. However, although there are many types of antibacterial spray products on the market, most of them have a single function, which is limited to disinfection and antibacterial, which is conducive to meeting people's various needs. Therefore, the development of an antibacterial spray product that integrates active ingredients of traditional Chinese medicine and has multiple functions has become an important demand that needs to be urgently addressed in the field of disinfection. Summary of the invention

[0003] In view of this, the present invention aims to provide an antibacterial spray with the effects of antibacterial, moisturizing, skin care, anti-inflammatory and tranquilizing.

[0004] To achieve the above object, the technical solution of the present invention is achieved as follows:

[0005] An antibacterial spray, the raw materials of which include rhodiola rosea extract, saffron extract, radix pubescens extract, bone-smashing extract, angelica extract, clematis extract, ligusticum chuanxiong extract, herba radix schizonepetae extract, herba striatae extract, salvia miltiorrhizae extract, cassia twig extract, turpentine, borneol, trichlorohydroxydiphenyl ether, lavender extract and auxiliary liquid.

[0006] Furthermore, the raw materials of the antibacterial spray are respectively as follows by weight percentage: 0.01%-0.5% Rhodiola rosea extract, 0.01%-0.4% Saffron extract, 0.01%-0.8% Lamiophloea chinensis extract, 0.01%-0.3% Bone-smacking extract, 0.01%-0.3% Angelica sinensis extract, 0.01%-0.3% Clematis chinensis extract, 0.01%-0.3% Chuanxiong extract, 0.01%-0.3% Polygonum aviculare extract, 0.01%-0.3% Lycopodiella ciliata extract, 0.01%-0.3% Salvia miltiorrhiza extract, 0.01%-0.3% Cassia twig extract, 0.1%-0.3% turpentine, 0.3%-0.6% borneol, 0.1%-0.25% trichlorohydroxydiphenyl ether, 0.3%-0.5% lavender extract, and the rest are auxiliary liquids.

[0007] Furthermore, the auxiliary liquid includes ethanol and purified water, and by weight percentage: ethanol 25%-40%, and the rest is purified water.

[0008] The main components of Rhodiola rosea are salidroside, tyrosol and gallic acid, which have antioxidant and anti-inflammatory effects and prevent skin diseases. The main components of saffron are flavonoids and their glycoside compounds, which have anti-inflammatory, antioxidant, anti-allergic and antiviral effects. The main components of Lamiophlomis odorifera are luteolin and total iridoid glycosides, which have hemostatic, analgesic and anti-inflammatory effects. Other medicinal extracts also have anti-inflammatory and analgesic, antibacterial and antibacterial effects, as well as the effects of dispelling wind and dampness, dredge meridians and relieve pain, and detoxify and reduce swelling. These medicinal extracts are widely used in daily necessities, food industry and medicine.

[0009] The antibacterial spray described in the present invention can integrate multiple natural active substance components by fusing multiple Chinese medicine extracts such as Rhodiola rosea, saffron, Lamiophloea chinensis and Bone-suppressed Bone. The components of each Chinese medicine extract solution have clear efficacy, are safe and have no toxic side effects. Natural Chinese herbal medicines can be fully utilized, which is beneficial to improving resource utilization and improving the antibacterial, anti-inflammatory, skin care and tranquilizing effects of the product, thereby meeting people's various needs for sprays and having better product strength.

[0010] Secondly, the main solvent system of the present invention is Chinese medicine extraction solution, ethanol and water. No chemical essence is added. Only lavender extract and Chinese medicine borneol are added to make the product rich in fresh, cool and sweet smell, mild to the skin, safe and non-irritating, and harmless to human skin. Moreover, the required preparation process is mature, the equipment investment is small, the overall system is stable, and batch production can be quickly realized.

[0011] In addition, the present invention also provides a method for preparing the antibacterial spray as described above, comprising:

[0012] Mixing Rhodiola rosea extract, saffron extract, Lamiophloea lappa extract, Boneless Plant extract, Angelica sinensis extract, Clematis chinensis extract, Ligusticum chuanxiong extract, Herba schizonepetae radix, Herba Lycopodii extract, Salvia miltiorrhizae extract, and Cinnamomum cassiae extract to obtain a mixed solution A;

[0013] Mix turpentine, borneol, trichlorohydroxydiphenyl ether and lavender extract, and stir to obtain a mixed solution B;

[0014] The obtained mixed solution A and auxiliary liquid are mixed and stirred, and then the mixed solution B is added and stirred. After standing, an antibacterial spray product is obtained.

[0015] Furthermore, the turpentine oil, the borneol, the trichlorohydroxy diphenyl ether and the lavender extract are mixed according to weight percentage, and stirred to obtain the mixed solution B, including: mixing the turpentine oil, the borneol, the trichlorohydroxy diphenyl ether and the lavender extract, and stirring at 20-26°C to obtain the mixed solution B.

[0016] Furthermore, the stirring time is between 30-45 minutes.

[0017] Furthermore, the obtained mixed liquid A and the auxiliary liquid are mixed, stirred, and then the mixed liquid B is added for stirring, and after standing, the antibacterial spray product is obtained, including: the obtained mixed liquid A and the auxiliary liquid are mixed, stirred at 20-26°C, and then the mixed liquid B is slowly added for stirring, and after standing, the antibacterial spray product is obtained; and / or, purified water and ethanol are mixed to obtain the auxiliary liquid.

[0018] Furthermore, the duration of the two stirrings is between 30-60 minutes; and / or the standing time is between 1-36 hours.

[0019] The preparation method of the antibacterial spray described in the present invention is not only simple and easy to operate, but also greatly shortens the preparation time, which is conducive to improving the preparation efficiency. It does not need to use high-end production equipment such as homogenizers, and can achieve sufficient and uniform mixing, which is conducive to reducing costs. At the same time, it can also well protect the natural medicinal active ingredients in Chinese herbal medicines, so that the prepared antibacterial spray product has multiple functions such as antibacterial, moisturizing, anti-inflammatory and skin care, and has no organic solvent residue, and is green and additive-free.

[0020] In addition, the present invention also provides the use of the antibacterial spray in inhibiting Staphylococcus aureus, Escherichia coli or Candida albicans, and the use of the antibacterial spray in skin and mucous membrane hygiene.

[0021] The antibacterial spray of the present invention is applied to the hygiene of skin and mucous membranes, has no irritation to the skin, has good system stability, and has multiple functions such as antibacterial, moisturizing, anti-inflammatory and skin care.

[0022] The antibacterial spray of the present invention meets the requirements of the "Disinfection Technical Specifications" of the Ministry of Health, can inhibit and disinfect Staphylococcus aureus, Escherichia coli and Candida albicans, and can meet people's daily disinfection and sterilization needs. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] The accompanying drawings constituting a part of the present invention are used to provide a further understanding of the present invention. The exemplary embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute an improper limitation of the present invention. In the accompanying drawings:

[0024] Figure 1 The present invention is a flowchart of a method for preparing an antibacterial spray according to an embodiment of the present invention. DETAILED DESCRIPTION

[0025] It should be noted that, in the absence of conflict, the embodiments of the present invention and the features in the embodiments may be combined with each other.

[0026] The present invention will be described in detail below with reference to the accompanying drawings and in conjunction with embodiments.

[0027] The present embodiment relates to an antibacterial spray, which can not only be used for disinfection and antibacterial, but also can meet people's needs such as skin care, moisturizing, anti-inflammatory and calming, and has better use effect and product strength.

[0028] In terms of overall design, the antibacterial spray of this embodiment comprises raw materials including Rhodiola rosea extract, saffron extract, Lamiophloea chinensis extract, Bone-smashed Poria extract, Angelica sinensis extract, Clematis chinensis extract, Ligusticum chuanxiong extract, Herba schizonepetae radix, Herba Lycopodii extract, Salvia miltiorrhizae extract, Cassia twig extract, turpentine, borneol, trichlorohydroxydiphenyl ether, lavender extract and auxiliary liquid.

[0029] At this time, as designed above, a variety of Chinese herbal extracts such as Rhodiola rosea, saffron, Du Fu, Bone-buckle, Angelica sinensis, Clematis chinensis, Ligusticum chuanxiong, Herba schizonepetae, Herba Lycopodii, Salvia miltiorrhizae and cinnamon twigs were selected, which contain organic compounds such as alcohols, aldehydes, acids, phenols, lipids, ketones and terpenes. These compounds work together to achieve a synergistic effect, enhance the inhibitory and bactericidal activity against Staphylococcus aureus, Escherichia coli, Candida albicans, etc., enhance the calming of human skin, and achieve the effects of moisturizing, skin care, tranquilizing the nerves and anti-inflammatory.

[0030] Specifically, alcohols in sprays may have antibacterial, anti-inflammatory, immune-enhancing and spirit-boosting effects. They are mild to the skin, non-toxic, and can help improve the skin environment and promote blood circulation. Aldehydes have moisturizing, antibacterial and skin-hydrating effects. They can also metabolize the body's waste toxins and balance emotions. However, it should be noted that since some aldehydes may be irritating to the skin, the dosage must be controlled when used. Esters can have antispasmodic, anti-inflammatory, soothing nervous system and stimulating skin cell regeneration effects. They can also increase the amount of collagen, soften scar tissue, and have wrinkle removal and anti-aging effects. Moreover, esters are one of the safest and mildest chemical ingredients and will not irritate the skin.

[0031] Phenols can have whitening, strong stimulation of female hormones and antibacterial effects. However, it should be noted that they are irritating to the skin and need to be diluted and the dosage controlled when used to avoid adverse reactions caused by excessive use. Lipids (or esters) mainly play the role of skin care, anti-aging and soothing the nervous system. The right amount of ketones can have therapeutic effects such as anti-inflammatory, anti-allergic and stabilizing cell membranes. Terpenes are important components in plant essential oils, including monoterpenes and sesquiterpenes. They may have multiple functions in sprays, such as detoxification, antibacterial, expectorant and digestion promotion. Sesquiterpenes, in particular, can directly penetrate the blood-brain barrier in a specific way, providing new treatment ideas for the treatment of brain diseases.

[0032] Moreover, it is worth mentioning that the main components of Rhodiola rosea extract include salidroside, tyrosol and gallic acid, etc., which have antioxidant and anti-inflammatory effects, and have a preventive effect on skin diseases. The main components of saffron include flavonoids and their glycoside compounds, which have anti-inflammatory, antioxidant, anti-allergic and antiviral effects. The main components of Duyiwei include luteolin and total iridoid glycosides, which have hemostatic, analgesic and anti-inflammatory effects. The main active substance of Drynaria extract is naringin, and it also contains methyl eugenol, new North American erythrin, protocatechuic acid, bone broken dihydroflavonoids and other substances. It has multiple effects such as anti-inflammatory, anti-allergic, anti-aging and whitening. The main components of Angelica extract include volatile oils, flavonoids, sugars, organic acids, etc., which have multiple effects such as blood tonifying and blood circulation, regulating menstruation and relieving pain, moistening the intestines and laxatives, nourishing blood and calming the nerves.

[0033] The extract of Clematis chinensis contains various ingredients such as anemonin, anemonin lactone, sterols, sugars, saponins, etc., and has various pharmacological effects such as analgesia, anti-inflammatory, anti-diuretic, choleretic, and antibacterial. The extract of Ligusticum chuanxiong mainly contains effective ingredients such as phthalide, alkaloids, phenolic acid, polysaccharides, chuanxiongzine, and ferulic acid, and has various effects such as anti-inflammatory, antioxidant and antibacterial effects, whitening and moisturizing the skin. The main ingredients of the extract of Herba pelargonidis include extracts of Chinese medicinal materials such as Artemisia argyi, Panax notoginseng, safflower, Herba pelargonidis, Herba spatholobi (Shujincao), Angelica dahurica, Notopterygium wilfordii, Ligusticum chuanxiong, Caulis sanguinea, and Angelica sinensis, and have multiple effects such as promoting blood circulation, unblocking meridians, dispelling cold, removing dampness, relieving pain, reducing swelling, and promoting wound healing. The extract of Herba pelargonidis mainly contains the effective ingredients of Herba pelargonidis, and has multiple effects such as anti-inflammatory, antipyretic, analgesia, dispelling wind and dampness, relaxing muscles and activating meridians, and dispelling cold and relieving pain.

[0034] Danshen extract mainly includes tanshinone, tanshinone acid, cryptotanshinone, danshensu, rosmarinic acid and other ingredients, which have multiple functions such as promoting blood circulation and removing blood stasis, calming the nerves, anti-oxidation, anti-bacterial and anti-inflammatory, promoting blood circulation, etc. Cinnamon twig extract mainly contains volatile oils (such as cinnamaldehyde, cinnamonic acid, cinnamyl acetate, phenylpropyl acetate, etc.), phenols, organic acids, polysaccharides, glycosides, coumarins and tannins, etc., which have antipyretic and cooling, antibacterial and anti-inflammatory, digestive enhancement, analgesic and anticonvulsant, anti-aging and other effects.

[0035] Turpentine has the effects of antibacterial, antifungal, promoting wound healing, relieving muscle pain and improving skin itching. Turpentine contains a variety of active ingredients, such as eucalyptol and α-pinene, which can interfere with the synthesis of bacterial cell walls or destroy the structure of bacterial cell membranes, thereby inhibiting the growth and reproduction of bacteria and fungi. Trichlorohydroxydiphenyl ether can play an antibacterial, disinfecting and deodorizing role.

[0036] The effects of borneol in spraying mainly include clearing away heat and detoxifying, reducing swelling and relieving pain, preserving and promoting tissue regeneration, and awakening the mind. Borneol is slightly cold in nature, pungent and bitter in taste, and is applicable to the heart, spleen, and lung meridians. It has the effects of clearing away heat and detoxifying, and can relieve symptoms such as sores, swelling and pain, sore throat, and sores in the mouth and tongue caused by heat toxins. Moreover, borneol also has the effects of reducing swelling and relieving pain, and can be used to relieve discomfort symptoms such as local swelling, pain, etc. caused by traumatic injuries. The preservative and promoting tissue regeneration effects of borneol make it have certain effects in treating sores, swelling and pain, burns, scalds, etc. At the same time, borneol can also awaken the mind and be used to relieve symptoms such as fever coma, stroke phlegm, and qi depression. Other medicinal material extracts also have anti-inflammatory and analgesic, antibacterial, antibacterial effects, wind and dampness removal, collateral dredging and pain relief, and the effects of detoxification and swelling reduction. These medicinal material extracts are widely used in daily necessities, food industry, and medical fields.

[0037] The main effects of lavender extract in spray include soothing and relaxing the body and mind, promoting sleep and improving sleep quality. Lavender extract has a unique fragrance, which is believed to have a certain soothing and relaxing effect, which can help people relieve tension and anxiety, thus helping to fall asleep faster and improve sleep quality to a certain extent.

[0038] Therefore, the antibacterial spray of this embodiment, by including the above-mentioned raw materials, can promote and synergize each other among the raw materials, and has the effect of "1+1>2", and has the effects of antibacterial, moisturizing, skin care, anti-inflammatory and calming, thereby improving the single function of the antibacterial spray, so as to meet people's other needs such as anti-inflammatory, moisturizing, etc. in addition to disinfection and antibacterial. It can be used to inhibit Staphylococcus aureus, Escherichia coli or Candida albicans, and can also be used in scenarios such as skin and mucous membrane hygiene.

[0039] The antibacterial spray of this embodiment can integrate multiple natural active substance components by integrating multiple Chinese medicine extracts such as Rhodiola rosea, saffron, Du Fu and Bone Mulberry, and each Chinese medicine extract solution has a clear efficacy, is safe and has no toxic side effects, and can fully utilize natural Chinese herbal medicines, which is beneficial to improving resource utilization and enhancing the product's antibacterial, anti-inflammatory, moisturizing, skin care and calming effects.

[0040] Secondly, the main solvent system of the present invention is Chinese medicine extraction solution, ethanol and water. No chemical essence is added. Only lavender extract and Chinese medicine borneol are added to make the product rich in fresh, cool and sweet smell, mild to the skin, safe and non-irritating, and harmless to human skin. Moreover, the required preparation process is mature, the equipment investment is small, the overall system is stable, and batch production can be quickly realized.

[0041] In specific implementation, as a preferred implementation form, the raw materials of the antibacterial spray of this embodiment are respectively as follows by weight percentage:

[0042] Rhodiola rosea extract 0.01%-0.5%, saffron extract 0.01%-0.4%, Lamiophloea chinensis extract 0.01%-0.8%, Bone-smacking extract 0.01%-0.3%, Angelica sinensis extract 0.01%-0.3%, Clematis chinensis extract 0.01%-0.3%, Ligusticum chuanxiong extract 0.01%-0.3%, Herba schizonepetae radix 0.01%-0.3%, Herba Lycopodii herba schizonepetae extract 0.01%-0.3%, Salvia miltiorrhizae extract 0.01%-0.3%, Cinnamomum cassiae extract 0.01%-0.3%, turpentine 0.1%-0.3%, borneol 0.3%-0.6%, trichlorohydroxydiphenyl ether 0.1%-0.25%, lavender extract 0.3%-0.5%, and the rest are auxiliary liquids. Here, the auxiliary liquid preferably includes ethanol and purified water, and by weight percentage: ethanol 25%-40%, and the rest is purified water.

[0043] Among them, turpentine oil can be specifically used at 0.2%, borneol can be specifically used at 0.5%, trichlorohydroxydiphenyl ether can be specifically used at 0.17%, lavender extract can be specifically used at 0.4%, and ethanol can be specifically used at 30%, so as to facilitate uniform mixing of the raw materials and make the antibacterial spray have a fresh, cool and sweet smell.

[0044] Of course, any product implementing the present invention does not necessarily need to achieve all the advantages described above at the same time. The antibacterial spray of this embodiment is mainly to solve the problem that the existing antibacterial spray has a single function and can only disinfect and inhibit bacteria. Some existing antibacterial sprays even have odor stimulation, nerve stimulation, and are not friendly to the skin, which is not conducive to meeting people's needs for skin care, calming the nerves, etc. in addition to disinfection and inhibition of bacteria.

[0045] In addition, the present invention also provides a method for preparing an antibacterial spray, such as Figure 1 As shown, the preparation method comprises:

[0046] S1, mixing Rhodiola rosea extract, Saffron extract, Lamiophloea lappa extract, Boneless Plant extract, Angelica sinensis extract, Clematis chinensis extract, Ligusticum chuanxiong extract, Herba schizonepetae radix, Herba Lycopodii extract, Salvia miltiorrhizae extract, and Cinnamomum cassiae extract according to weight percentage to obtain a mixed solution A;

[0047] S2, mixing turpentine, borneol, trichlorohydroxydiphenyl ether and lavender extract according to weight percentage, stirring, and obtaining a mixed solution B;

[0048] S3. Mix the obtained mixed solution A and auxiliary liquid, stir them, then add the mixed solution B and stir them. After standing, an antibacterial spray product is obtained.

[0049] As a preferred implementation form, in S2, turpentine, borneol, trichlorohydroxy diphenyl ether and lavender extract are mixed according to weight percentage (i.e., the weight percentage of the above-mentioned separator materials), and stirred at room temperature (20-26° C.) to obtain a mixed solution B. In addition, the stirring time may preferably be between 30-45 minutes, and for example, may be specifically 35 minutes, 40 minutes or 45 minutes, so that the turpentine, borneol, trichlorohydroxy diphenyl ether and lavender extract are fully mixed.

[0050] As a preferred implementation form, purified water and ethanol are mixed according to weight percentage to obtain an auxiliary liquid. At the same time, also as a preferred implementation form, in S3, the obtained mixed liquid A and the auxiliary liquid are mixed, stirred at room temperature (20-26° C.), and then the mixed liquid B is slowly added for stirring, and after standing, an antibacterial spray product is obtained. The room temperature can be specifically 23° C. or 25° C. to facilitate mixing of the raw materials.

[0051] During specific implementation, in S3, the duration of the two stirrings can be preferably set between 30-60 min, and for example, can be specifically 40 min, 45 min or 55 min, etc., so as to facilitate the mixed solution A and mixed solution C to be fully mixed, and then fully mixed with mixed solution B. In addition, the standing time is preferably set between 1-36 h, and for example, can be specifically 3 h, 8 h, 12 h, 24 h or 30 h, etc., so as to allow various natural active ingredients to be fully mixed and uniform, and to ensure the stability and use effect of the spray. Of course, standing still also helps to observe whether unstable phenomena such as stratification and precipitation occur in the mixed liquid, so as to deal with them in time. If these problems occur in the spray after standing still, it may mean that the formula needs to be adjusted or the production process needs to be improved.

[0052] The preparation method of the antibacterial spray of the present embodiment is not only simple and easy to operate, but also greatly shortens the preparation time, which is conducive to improving the preparation efficiency. It also does not need to use high-end production equipment such as homogenizers to achieve sufficient and uniform mixing, which is conducive to reducing costs. At the same time, it can also provide good protection for the natural medicinal active ingredients in Chinese herbal medicines, so that the prepared antibacterial spray product has multiple functions such as antibacterial, moisturizing, anti-inflammatory and skin care, and has no organic solvent residue, and is green and additive-free.

[0053] Here, the present invention is further described in conjunction with specific embodiments:

[0054] Example 1

[0055] An antibacterial spray, wherein the raw materials are respectively as follows by weight percentage:

[0056] Rhodiola rosea extract 0.01%, saffron extract 0.01%, Lamiophloea chinensis extract 0.01%, Bone-smacking extract 0.01%, Angelica sinensis extract 0.01%, Clematis chinensis extract 0.01%, Ligusticum chuanxiong extract 0.01%, Herba Polygoni Multiflori extract 0.01%, Herba Lycopodii Herba Scimicifugae extract 0.01%, Salvia miltiorrhizae extract 0.01%, Cinnamomum cassiae extract 0.01%, turpentine oil 0.2%, borneol 0.5%, trichloroethylene 0.17%, lavender extract 0.4%, ethanol 30%, purified water 68.62%.

[0057] The preparation method of the antibacterial spray comprises:

[0058] Fully dissolve 0.01% Rhodiola rosea extract, 0.01% Saffron extract, 0.01% Lamiophloea chinensis extract, 0.01% Boneless Plant extract, 0.01% Angelica sinensis extract, 0.01% Clematis chinensis extract, 0.01% Ligusticum chuanxiong extract, 0.01% Polygonum aviculare extract, 0.01% Lycopodiella cuneata extract, 0.01% Salvia miltiorrhiza extract, and 0.01% Cinnamomum cassia extract to prepare a mixed solution A.

[0059] Subsequently, 0.2% turpentine, 0.5% borneol, 0.17% trichlorohydroxydiphenyl ether, and 0.4% lavender extract are mixed and stirred at room temperature for 30-45 minutes to obtain a mixed solution B; at the same time, 68.62% purified water and 30% ethanol are mixed to fully dissolve each other to obtain an auxiliary liquid. Finally, the obtained mixed solution A and the auxiliary liquid are mixed with each other, stirred at room temperature for 30-60 minutes, and then the obtained mixed solution B is slowly added to the above solution, stirred for 30-60 minutes, and left to stand for 1-36 hours to obtain an antibacterial spray product.

[0060] Example 2

[0061] An antibacterial spray, wherein the raw materials are respectively as follows by weight percentage:

[0062] 0.1% Rhodiola rosea extract, 0.1% saffron extract, 0.1% Lamiophloea chinensis extract, 0.1% Bone-fried Bone-smashed extract, 0.1% Angelica sinensis extract, 0.1% Clematis chinensis extract, 0.1% Ligusticum chuanxiong extract, 0.1% Polygonum aviculare extract, 0.1% Lycopodiella cuneata extract, 0.1% Salvia miltiorrhiza extract, 0.1% Cassia twig extract, 0.2% turpentine, 0.5% borneol, 0.17% trichloroethylene, 0.4% lavender extract, 30% ethanol, and 68.18% purified water.

[0063] The preparation method of the antibacterial spray comprises:

[0064] Fully dissolve 0.1% Rhodiola rosea extract, 0.1% Saffron extract, 0.1% Lamiophloea chinensis extract, 0.1% Boneless Plant extract, 0.1% Angelica sinensis extract, 0.1% Clematis chinensis extract, 0.1% Ligusticum chuanxiong extract, 0.1% Polygonum aviculare extract, 0.1% Lycopodiella cernua extract, 0.1% Salvia miltiorrhiza extract, and 0.1% Cinnamomum cassia extract to prepare a mixed solution A.

[0065] Subsequently, 0.2% turpentine, 0.5% borneol, 0.17% trichlorohydroxydiphenyl ether and 0.4% lavender extract are mixed and stirred at room temperature for 30-45 minutes to obtain a mixed solution B; at the same time, 68.18% purified water and 30% ethanol are mixed to fully dissolve each other to obtain an auxiliary liquid. Finally, the obtained mixed solution A and the auxiliary liquid are mixed with each other, stirred at room temperature for 30-60 minutes, and then the obtained mixed solution B is slowly added to the above solution, stirred for 30-60 minutes, and left to stand for 1-36 hours to obtain an antibacterial spray product.

[0066] Example 3

[0067] An antibacterial spray, wherein the raw materials are respectively as follows by weight percentage:

[0068] 0.2% Rhodiola rosea extract, 0.2% saffron extract, 0.2% Lamiophloea chinensis extract, 0.2% Bone-fried bone extract, 0.2% Angelica sinensis extract, 0.2% Clematis extract, 0.2% Ligusticum chuanxiong extract, 0.2% Polygonum aviculare extract, 0.2% Lycopodiella cuneata extract, 0.2% Salvia miltiorrhiza extract, 0.2% Cassia twig extract, 0.2% turpentine, 0.5% borneol, 0.17% trichloroethylene, 0.4% lavender extract, 30% ethanol, and 66.53% purified water.

[0069] The preparation method of the antibacterial spray comprises:

[0070] Fully dissolve 0.2% Rhodiola rosea extract, 0.2% Saffron extract, 0.2% Lamiophloea chinensis extract, 0.2% Boneless Plant extract, 0.2% Angelica sinensis extract, 0.2% Clematis chinensis extract, 0.2% Ligusticum chuanxiong extract, 0.2% Polygonum aviculare extract, 0.2% Lycopodiella cuneata extract, 0.2% Salvia miltiorrhiza extract, and 0.2% Cinnamomum cassia extract to prepare a mixed solution A.

[0071] Subsequently, 0.2% turpentine, 0.5% borneol, 0.17% trichlorohydroxydiphenyl ether and 0.4% lavender extract are mixed and stirred at room temperature for 30-45 minutes to obtain a mixed solution B; at the same time, 66.53% purified water and 30% ethanol are mixed to fully dissolve each other to obtain an auxiliary liquid. Finally, the obtained mixed solution A and the auxiliary liquid are mixed with each other, stirred at room temperature for 30-60 minutes, and then the obtained mixed solution B is slowly added to the above solution, stirred for 30-60 minutes, and left to stand for 1-36 hours to obtain an antibacterial spray product.

[0072] Example 4

[0073] An antibacterial spray, wherein the raw materials are respectively as follows by weight percentage:

[0074] 0.3% Rhodiola rosea extract, 0.3% saffron extract, 0.3% Lamiophloea chinensis extract, 0.3% Bone-fried Poria extract, 0.3% Angelica sinensis extract, 0.3% Clematis chinensis extract, 0.3% Ligusticum chuanxiong extract, 0.3% Polygonum aviculare extract, 0.3% Lycopodiella cuneata extract, 0.3% Salvia miltiorrhiza extract, 0.3% cinnamon twig extract, 0.2% turpentine, 0.5% borneol, 0.17% trichloroethylene, 0.4% lavender extract, 30% ethanol, and 65.43% purified water.

[0075] The preparation method of the antibacterial spray comprises:

[0076] Fully dissolve 0.3% Rhodiola rosea extract, 0.3% Saffron extract, 0.3% Lamiophloea chinensis extract, 0.3% Bone-smashed Poria extract, 0.3% Angelica sinensis extract, 0.3% Clematis chinensis extract, 0.3% Ligusticum chuanxiong extract, 0.3% Polygonum aviculare extract, 0.3% Lycopodiella cuneata extract, 0.3% Salvia miltiorrhiza extract, and 0.3% Cinnamomum cassia extract to prepare a mixed solution A.

[0077] Subsequently, 0.2% turpentine, 0.5% borneol, 0.17% trichlorohydroxydiphenyl ether and 0.4% lavender extract are mixed and stirred at room temperature for 30-45 minutes to obtain a mixed solution B; at the same time, 65.43% purified water and 30% ethanol are mixed to fully dissolve each other to obtain an auxiliary liquid. Finally, the obtained mixed solution A and the auxiliary liquid are mixed with each other, stirred at room temperature for 30-60 minutes, and then the obtained mixed solution B is slowly added to the above solution, stirred for 30-60 minutes, and left to stand for 1-36 hours to obtain an antibacterial spray product.

[0078] Example 5

[0079] An antibacterial spray, wherein the raw materials are respectively as follows by weight percentage:

[0080] 0.5% Rhodiola rosea extract, 0.4% saffron extract, 0.8% Lamiophloea chinensis extract, 0.3% Bone-fried bone extract, 0.3% Angelica sinensis extract, 0.3% Clematis chinensis extract, 0.3% Ligusticum chuanxiong extract, 0.3% Polygonum aviculare extract, 0.3% Lycopodiella cuneata extract, 0.3% Salvia miltiorrhiza extract, 0.3% cinnamon twig extract, 0.2% turpentine, 0.5% borneol, 0.17% trichloroethylene, 0.4% lavender extract, 30% ethanol, and 64.63% purified water.

[0081] The preparation method of the antibacterial spray comprises:

[0082] Fully dissolve 0.5% Rhodiola rosea extract, 0.4% Saffron extract, 0.8% Lamiophloea chinensis extract, 0.3% Bone-sucking extract, 0.3% Angelica sinensis extract, 0.3% Clematis chinensis extract, 0.3% Ligusticum chuanxiong extract, 0.3% Polygonum aviculare extract, 0.3% Lycopodiella cernua extract, 0.3% Salvia miltiorrhiza extract, and 0.3% Cinnamomum cassia extract to prepare a mixed solution A.

[0083] Subsequently, 0.2% turpentine, 0.5% borneol, 0.17% trichlorohydroxy diphenyl ether and 0.4% lavender extract are mixed and stirred at room temperature for 30-45 minutes to obtain a mixed solution B; at the same time, 64.63% purified water and 30% ethanol are mixed to fully dissolve each other to obtain an auxiliary liquid. Finally, the obtained mixed solution A and the auxiliary liquid are mixed with each other, stirred at room temperature for 30-60 minutes, and then the obtained mixed solution B is slowly added to the above solution, stirred for 30-60 minutes, and left to stand for 1-36 hours to obtain an antibacterial spray product.

[0084] Antibacterial test of antibacterial spray

[0085] Experimental methods:

[0086] Staphylococcus aureus, Escherichia coli, and Candida albicans were cultured in culture medium and used in the experiment.

[0087] The antibacterial liquid samples in Examples 1-5 were added to the sterilized PBS buffer for 2 min, 5 min, 10 min, and 20 min, respectively, and then cultured at a suitable temperature after inoculation of the microbial culture solution.

[0088] In addition, a blank experimental liquid was used as a control group. The control group was inoculated with microorganisms in PBS buffer and no antibacterial substances were added. Since no antibacterial substances were added, the antibacterial rate of the control group can be regarded as 0, so it is not reflected in the following Tables 1 to 3.

[0089] Table 1: Staphylococcus aureus antibacterial test results

[0090]

[0091] Table 2: E. coli antibacterial test results

[0092]

[0093] Table 3: Candida albicans antibacterial test results

[0094]

[0095]

[0096] From the above data, the Chinese medicine extract of this embodiment, that is, the antibacterial spray, has antibacterial and anti-inflammatory effects. When the Chinese medicine extract is compounded in a certain proportion, the antibacterial effect on Staphylococcus aureus, Escherichia coli and Candida albicans can be improved. In particular, the raw materials in the antibacterial spray are respectively "Rhodiola rosea extract 0.5%, saffron extract 0.4%, Lamiophlomis extract 0.8%, bone shavings extract 0.3%, angelica extract 0.3%, clematis extract 0.3%, 0.3% of Chuanxiong extract, 0.3% of Radix Angelicae Pubescentis extract, 0.3% of Lycopodiella ciliata extract, 0.3% of Salvia miltiorrhiza extract, 0.3% of Cinnamomum cassia extract, 0.2% of turpentine, 0.5% of Borneol, 0.17% of Trichlorobenzene, 0.4% of Lavender extract, 30% of Ethanol, and 64.63% of Purified Water. ", the antibacterial effect of the antibacterial spray of this embodiment on Staphylococcus aureus, Escherichia coli and Candida albicans can reach 99.99% in 2 minutes, and the antibacterial effect is more obvious.

[0097] Of course, it is worth mentioning that PBS buffer, the full name of which is phosphate buffered saline, is a buffer solution widely used in biochemical research. The main components of PBS buffer include Na2HPO4, KH2PO4, NaCl and KCl, which work together in the solution to maintain a stable pH environment. Because Na2HPO4 and KH2PO4 have secondary dissociation characteristics, the pH value range of PBS buffer is wide and can adapt to different experimental requirements. At the same time, the addition of NaCl and KCl is mainly to increase the salt ion concentration and further stabilize the solution environment. PBS buffer has many uses in biological research. It is isotonic and non-toxic to cells, so it is often used to dilute substances, clean cell containers, and wash cells and tissues. In addition, because its osmotic pressure and ion concentration are usually close to the pH of the human body, PBS buffer is also often used to simulate the human body environment and conduct related biochemical experiments.

[0098] Skin test of antibacterial spray:

[0099] Experimental methods:

[0100] Three male white rabbits were selected. 24 hours before the experiment, the hair on both sides of the spine of the animals were removed. The hair removal area was 3cm×3cm, and the epidermis should not be damaged.

[0101] Take 0.5 mL of the antibacterial liquid in Example 5 as a sample and apply it directly to the hairless skin on one side of the animal (the other side serves as a blank control), with an application area of ​​2.5 cm×2.5 cm. 4 hours after application, wash with water or a suitable non-irritating solvent to remove the residue.

[0102] Apply once a day for 14 consecutive days. Observe the results 24 hours after each application and score according to the skin irritation reaction scoring standard of the "Disinfection Technical Specifications". After 14 consecutive days of application, no skin irritation reaction occurred. The test scores are shown in Table 4 below:

[0103] Table 4 Skin test results

[0104]

[0105] According to the data in the above table, according to the skin irritation intensity grading evaluation standard of the "Technical Specifications for Disinfection", it is determined that the antibacterial liquid (antibacterial spray) of this embodiment is non-irritating in multiple intact skin irritation tests on rabbits. In other words, the antibacterial liquid (antibacterial spray) of this application is mild to the skin, safe and non-irritating, and does not harm human skin.

[0106] At the same time, the antibacterial liquid (antibacterial spray) of this embodiment can be judged by smell to be rich in fresh, cool and sweet smells, and in the case of skin tests, no abnormal reaction of rabbits to the smell of the antibacterial liquid (antibacterial spray) was observed, which shows that the smell of the antibacterial liquid (antibacterial spray) of this embodiment is more easily accepted by people.

[0107] To this end, the antibacterial spray of this embodiment can be used to inhibit Staphylococcus aureus, Escherichia coli or Candida albicans, and can also be used in skin and mucous membrane hygiene. It has a wide range of usage scenarios and not only has antibacterial, anti-inflammatory and calming effects, but is also gentle on the skin, safe and non-irritating, does not harm human skin, is beneficial for moisturizing and skin care, and at the same time, is rich in fresh, cool and sweet smells, and has better use effects.

[0108] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. An antibacterial spray, characterized in that: The raw materials of the antibacterial spray include rhodiola rosea extract, saffron extract, radix ophiopogonis extract, bone-smashing extract, angelica extract, clematis extract, ligusticum chuanxiong extract, radix oleifera extract, herba striatae extract, herba striatae extract, salvia miltiorrhizae extract, cassia twig extract, turpentine, borneol, trichlorohydroxydiphenyl ether, lavender extract and auxiliary liquid.

2. The antibacterial spray according to claim 1, characterized in that: The raw materials of the antibacterial spray are respectively as follows by weight percentage: Rhodiola rosea extract 0.01%-0.5%, saffron extract 0.01%-0.4%, Lamiophloea chinensis extract 0.01%-0.8%, Bone-smacking extract 0.01%-0.3%, Angelica sinensis extract 0.01%-0.3%, Clematis chinensis extract 0.01%-0.3%, Ligusticum chuanxiong extract 0.01%-0.3%, Herba schizonepetae radix 0.01%-0.3%, Herba Lycopodii herba schizonepetae extract 0.01%-0.3%, Salvia miltiorrhizae extract 0.01%-0.3%, Cinnamomum cassiae extract 0.01%-0.3%, turpentine 0.1%-0.3%, borneol 0.3%-0.6%, trichlorohydroxydiphenyl ether 0.1%-0.25%, lavender extract 0.3%-0.5%, and the rest are auxiliary liquids.

3. The antibacterial spray according to claim 2, characterized in that: The auxiliary liquid comprises ethanol and purified water, and by weight percentage: ethanol is 25%-40%, and the rest is purified water.

4. A method for preparing the antibacterial spray according to any one of claims 1 to 3, characterized in that: include: Mixing Rhodiola rosea extract, saffron extract, Lamiophloea lappa extract, Boneless Plant extract, Angelica sinensis extract, Clematis chinensis extract, Ligusticum chuanxiong extract, Herba schizonepetae radix, Herba Lycopodii extract, Salvia miltiorrhizae extract, and Cinnamomum cassiae extract to obtain a mixed solution A; Mix turpentine, borneol, trichlorohydroxydiphenyl ether and lavender extract, and stir to obtain a mixed solution B; The obtained mixed solution A and auxiliary liquid are mixed and stirred, and then the mixed solution B is added and stirred. After standing, an antibacterial spray product is obtained.

5. The preparation method according to claim 4, characterized in that: The turpentine oil, the borneol, the trichlorohydroxy diphenyl ether and the lavender extract are mixed according to weight percentage, and stirred to obtain the mixed solution B, which includes: The turpentine oil, the borneol, the trichlorohydroxydiphenyl ether and the lavender extract are mixed, and stirred at 20-26° C. to obtain the mixed solution B.

6. The preparation method according to claim 5, characterized in that: The stirring time is between 30-45 min.

7. The preparation method according to claim 4, characterized in that: The obtained mixed solution A and the auxiliary liquid are mixed and stirred, and then the mixed solution B is added and stirred. After standing, the antibacterial spray product is obtained, including: The obtained mixed solution A and the auxiliary liquid are mixed, stirred at 20-26° C., and then the mixed solution B is slowly added and stirred, and after standing, the antibacterial spray product is obtained; and / or, The auxiliary liquid is obtained by mixing purified water and ethanol.

8. The preparation method according to claim 6, characterized in that: The duration of the two stirrings is between 30-60 minutes; and / or the standing time is between 1-36 hours.

9. The use of the antibacterial spray according to any one of claims 1 to 3, characterized in that: The antibacterial spray is used for inhibiting Staphylococcus aureus, Escherichia coli or Candida albicans.

10. The use of the antibacterial spray according to any one of claims 1 to 3, characterized in that: The antibacterial spray is used for skin and mucous membrane hygiene.

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