Polygonatum sibiricum amino acid primary pulp and preparation method thereof

Abstract

The invention discloses rhizoma polygonati amino acid primary pulp and a preparation method thereof, and relates to the field of traditional Chinese medicine beverages. The polygonatum sibiricum amino acid primary pulp is prepared from polygonatum sibiricum and other medicinal materials through enzymolysis, two times of extraction and homogeneous emulsification, and comprises a compound oil phase and a polygonatum sibiricum extracting solution water phase, wherein the compound oil phase is composed of plant oil rich in phytosterol and oily esters. The preparation method of the polygonatum sibiricum amino acid primary pulp is mild in condition, the storage stability of the extract is greatly improved, and a controlled release effect on effective substances is achieved; on the basis, the compound oil phase improves the extraction amount of saponin and flavonoid substances in the medicinal materials to a great extent.

Description

Technical Field

[0001] The invention relates to the field of traditional Chinese medicine beverages, and in particular to polygonatum amino acid puree and a preparation method thereof. Background Art

[0002] Polygonatum sibiricum, commonly known as chicken-head ginseng, tiger ginger, etc., is the dried rhizome of Yunnan Polygonatum sibiricum, Polygonatum sibiricum or Polygonatum sibiricum of the Liliaceae family. It has the effects of replenishing qi and nourishing yin, strengthening the spleen, moistening the lungs, and benefiting the kidneys. Polygonatum sibiricum contains a variety of active ingredients such as polysaccharides, saponins, flavonoids, etc., which have multiple effects such as lowering blood sugar, anti-bacterial and anti-inflammatory, antioxidant, and anti-fatigue. Polygonatum sibiricum is usually processed by soaking in rice wine or steaming and sun exposure to reduce its numb taste and weak toxicity. However, experiments have shown that the content of effective substances such as polysaccharides and saponins in Polygonatum sibiricum prepared by traditional methods has been reduced to varying degrees.

[0003] The Chinese patent with publication number CN113907234A discloses a raw polygonatum sibiricum pulp and its preparation method, which adopts the method of adding mannitol and performing high-pressure jet milling at 80-90°C to eliminate the numbness of the tongue and the tingling of the throat of raw polygonatum sibiricum. The polygonatum sibiricum processing technology represented by this patent maximizes the retention of the ingredients of the medicinal material while improving the taste, but also retains the ineffective ingredients in the medicinal material without any screening, which may interfere with the effective ingredients from exerting their medicinal value, and the effective ingredients in the medicinal material are prone to chemical changes under relatively rough processing, storage and consumption methods.

[0004] The technical solution disclosed in the above patents also adopts the compatibility of medicinal materials to improve the storage performance of Polygonatum sibiricum puree without adding preservatives. In order to solve the storage stability problem of medicinal material extract solutions, another more effective method in the prior art is to prepare them into emulsions. Emulsion is a surfactant system. Making emulsions with components of different oil / water affinities can improve their dispersibility, reduce the volatilization and oxidation loss of some components, and can also control the release of nutrients therein. Existing medicinal material emulsions usually use artificial emulsifiers, oil phases and / or additives that are not suitable for large quantities in food, and are used as external medicines and skin care products. Plant oil bodies are rich in protein-phospholipid layers, and natural emulsions can be easily formed without additional emulsifiers, providing clues for the preparation of Polygonatum sibiricum emulsion drinks. Summary of the invention

[0005] In order to overcome the deficiencies of the prior art, the present invention aims to provide a method for preparing a raw material of amino acids of polygonatum, using an oil phase mainly composed of plant oil bodies and a water phase of polygonatum extract to prepare an oil-in-water (o / w) edible emulsion; the method improves the stability of the extract and has a controlled release effect on the effective substances. On this basis, unexpectedly, the composite oil phase composed of plant oil bodies rich in phytosterols and oily esters greatly increases the extraction amount of saponins and flavonoids in the medicinal material.

[0006] The present invention provides a polygonatum amino acid puree, which is a stable emulsion with a pH value between 6.5 and 8.0 and an ion concentration of no more than 30 mmol / L in terms of sodium ions. The polygonatum amino acid puree comprises the following raw materials in parts by weight: 4 to 7 parts of Chinese medicinal materials, 80 to 88 parts of water, 4 to 8 parts of plant oil bodies, 0.1 to 0.5 parts of oily esters, 0.01 to 0.1 parts of antioxidants, 6 to 12 parts of stabilizers and 0.1 to 0.5 parts of amino acid additives.

[0007] The present invention also provides a method for preparing the above-mentioned polygonatum amino acid puree, comprising the following steps:

[0008] The Chinese medicinal materials are added with water, and the enzyme is killed after enzymolysis to obtain an enzymolysis liquid;

[0009] The enzymatic hydrolysis liquid is heated and crudely extracted to obtain a crude extract;

[0010] Adding plant oil and oily esters to the crude extract, heating for refined extraction, filtering out the drug residue, and obtaining a refined extract;

[0011] Antioxidants, stabilizers and amino acid additives are added to the extract, and the extract is emulsified to obtain the polygonatum amino acid puree.

[0012] The technical solution of the present invention has the following beneficial effects:

[0013] 1. The method for preparing Polygonatum sibiricum amino acid puree of the present invention uses plant oil bodies and oily esters as the oil phase, utilizes plant proteins and phospholipids in the plant oil bodies, and can prepare an emulsion without an additional emulsifier, thereby improving the stability of the extract. During heating, storage, etc., the loss of effective substances is much lower than that of ordinary extracts.

[0014] 2. The method for preparing amino acid puree of Polygonatum sibiricum described in the present invention adopts a water-oil two-phase extractant to extract Chinese medicinal materials, wherein no alcohol is added to the water phase to ensure its extraction ability for polysaccharides and edible cleanliness. At the same time, the presence of the composite oil phase greatly improves the affinity of the extractant for other effective substances such as saponins and flavonoids, and the comprehensive extraction rate of effective ingredients of various medicinal materials is better than that of the prior art.

[0015] 3. The polygonatum amino acid puree of the present invention maintains the stability of gradual dissolution in simulated digestion, reflecting the controlled release effect of the effective substances therein, so that it can improve the absorption and utilization rate of these effective substances by users.

[0016] 4. The method for preparing amino acid puree of polygonatum according to the present invention is carried out under relatively mild processing conditions such as temperature and pH throughout the process, thereby reducing the decomposition and denaturation of various effective substances in the medicinal materials. The proportion of sapogenin in total saponins is greatly reduced compared with the polygonatum extract prepared by the traditional method, indicating that the saponins are less hydrolyzed. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 The figure is a grouped bar chart of the average particle size of the emulsion of the polygonatum amino acid puree obtained in each embodiment and comparative example, with the controlled variable as the basis for grouping, wherein the color of embodiment 1 as the control item of each group is darkened;

[0018] Figure 2 This is a bar chart of average particle size grouping of the polygonatum amino acid puree obtained in Example 1 under different environmental changes, with the controlled variable as the basis for grouping, wherein the initial polygonatum amino acid puree is darker in color as the control item of each group;

[0019] Figure 3 It simulates the change in particle size of Polygonatum amino acid puree emulsion and the release of saponins during digestion. 0 to 180 minutes is the simulated gastric juice digestion, and 180 to 360 minutes is the simulated intestinal digestive juice digestion. DETAILED DESCRIPTION

[0020] In order to make the technical means, creative features, objectives and effects of the present invention easy to understand, the specific implementation of the present invention is further described below. Obviously, the embodiments described therein are only part of the implementation of the present invention, which is an explanation of the technical solution of the present invention rather than a limitation.

[0021] The present invention provides a polygonatum amino acid puree, which is a stable emulsion, with a pH value between 6.5 and 8.0, and an ion concentration of no more than 30 mmol / L in terms of sodium ions. The polygonatum amino acid puree comprises the following raw materials in parts by weight: 4 to 7 parts of Chinese medicinal materials, 80 to 88 parts of water, 4 to 8 parts of plant oil bodies, 0.1 to 0.5 parts of oily esters, 0.01 to 0.1 parts of antioxidants, 6 to 12 parts of stabilizers, and 0.1 to 0.5 parts of amino acid additives.

[0022] The pH value and ion concentration of the amino acid puree of Polygonatum sibiricum are controlled by the buffer solution: if the pH is extremely high or extremely low, the stability of the emulsion and the effective substances of the medicinal materials will be destroyed; if the pH is low, at about 4 to 6, it is close to the comprehensive isoelectric point of the oil body protein, resulting in a decrease in the electrostatic repulsion of the protein, making the emulsion unstable; if the ion concentration is too high, it will destroy the oil-water interface protected by the protein and reduce the stability of the emulsion. The ion concentration is measured in terms of sodium ions, which refers to the ion concentration required for sodium ions to achieve the same effect on the emulsion colloid as any non-hydrogen ions within the food safety range. Since the stabilizer of the present invention is preferably a phosphate (sodium) salt buffer solution, sodium ions are the only strong ions that commonly affect the stability of the emulsion.

[0023] Preferably, the polygonatum amino acid puree comprises the following raw materials in parts by weight: 6 parts of Chinese medicinal materials, 86 parts of water, 6 parts of vegetable oil bodies, 0.2 parts of oily esters, 0.05 parts of antioxidants, 7.75 parts of stabilizers and 0.2 parts of amino acid additives.

[0024] Furthermore, in the plant oil bodies, sterols account for 0.2-1% of the dry weight thereof, and oleosin accounts for 10-15% of the dry weight thereof.

[0025] Existing studies have revealed the reason why plant oil bodies can form natural water-in-oil emulsions, that is, the protein and phospholipid monolayers rich in them act as emulsifiers for dispersing a small amount of oil in a large amount of water. Among them, the protein in some plant oil bodies can account for more than 10% of its mass, which not only has surface activity, that is, emulsification, but also has a stabilizing effect on the emulsion and nutritional value.

[0026] Surprisingly, the phytosterols contained in the plant oil can help solubilize and stabilize the saponins, especially steroidal saponins, in Chinese medicinal materials such as Polygonatum sibiricum. It is well known to those skilled in the art that 3β-sterol can form insoluble complexes with steroidal saponins, but when the concentrations of sterols and saponins are very low, saponins do not form precipitation in the oil-water system containing sterols, but instead act as an emulsifier to connect the oil-water phases, thereby increasing the extraction rate of saponins under the interaction with sterols and having higher stability.

[0027] Furthermore, the oily ester is one or more of isopropyl myristate, ethyl oleate, and ethyl butyrate; the antioxidant is a combination of tocopherol and ascorbyl palmitate; the stabilizer is a phosphate buffer solution; and the amino acid additive is γ-aminobutyric acid.

[0028] The oily esters are all oil phase materials with a large emulsification range reported in the literature, and a small amount of addition can increase the solubility of polar substances in medicinal materials such as flavonoids; in addition, the oily esters are all edible flavorings specified in national standards, which can enhance the flavor and taste of the emulsion.

[0029] Furthermore, the Chinese medicinal materials, by mass, include 65-75% of polygonatum, 15-25% of wolfberry and 5-15% of cistanche; preferably, the Chinese medicinal materials, by mass, include 70% of polygonatum, 20% of wolfberry and 10% of cistanche.

[0030] The present invention provides a method for preparing the above-mentioned polygonatum amino acid puree, comprising the following steps:

[0031] The Chinese medicinal materials are added with water, and the enzyme is killed after enzymolysis to obtain an enzymolysis liquid;

[0032] The enzymatic hydrolysis liquid is heated and crudely extracted to obtain a crude extract;

[0033] Adding plant oil and oily esters to the crude extract, heating for refined extraction, filtering out the drug residue, and obtaining a refined extract;

[0034] Antioxidants, stabilizers and amino acid additives are added to the extract, and the extract is emulsified to obtain the polygonatum amino acid puree.

[0035] Furthermore, the enzymatic hydrolysis uses 2000-5000 U / g of composite enzyme, including 25-35% by mass of cellulase, and the remainder is papain; the enzyme inactivation method is ultrasonic enzyme inactivation.

[0036] The effective ingredients such as polysaccharides and saponins in Chinese medicinal materials such as Polygonatum sibiricum are easily hydrolyzed under high temperature and acid-base conditions, changing their medicinal properties and solubility. Therefore, the preparation method of the present invention is carried out under relatively mild temperature and pH conditions throughout the process. In order to compensate for the effect of lower extraction temperature on the extraction efficiency of polysaccharides, the preparation method of the present invention uses enzymatic hydrolysis to pretreat Chinese medicinal materials, wherein the enzyme is inactivated by ultrasound below 50kHz, and the temperature of the enzymatic hydrolyzate during the process is usually not more than 50°C.

[0037] Furthermore, the crude extraction time is 1.5 to 3 hours, and the feed liquid temperature is 65 to 75° C.; the fine extraction time is 2.5 to 4 hours, and the feed liquid temperature is 50 to 65° C. Preferably, the crude extraction time is 2 hours, and the feed liquid temperature is 70° C.; the fine extraction time is 3 hours, and the feed liquid temperature is 60° C.

[0038] The first crude extraction is mainly for polysaccharides in Chinese medicinal materials such as Polygonatum sibiricum. Therefore, a higher liquid temperature and a shorter extraction time are used to ensure that the polysaccharide extraction rate is increased as much as possible while controlling the loss of effective ingredients. The second fine extraction is mainly for saponins, flavonoids and other components with relatively low polarity in the medicinal materials. At the same time, in order to allow the oil phase to fully absorb water for subsequent emulsification, the stirring extraction time is extended.

[0039] Furthermore, the emulsification is carried out in a homogenizing device, the rotor speed of which is 8000-12000 r / min, and the emulsification time is 5-10 min. Preferably, the rotor speed of the homogenizing device is 11000 r / min, and the emulsification time is 7 min.

[0040] The homogenizing equipment includes but is not limited to a high-speed homogenizer, a high-pressure homogenizer or a colloid mill. Since a large amount of heat is generated during the operation of the homogenizing equipment, the emulsion temperature should be controlled to be no more than 70°C during the emulsification process.

[0041] The present invention is described in detail below with reference to the examples. The experimental methods are conventional methods unless otherwise specified; the reagents and materials are commercially available unless otherwise specified.

[0042] Example 1

[0043] Prepare Polygonatum amino acid puree as follows:

[0044] (1) Enzymolysis: Weigh dried Polygonatum sibiricum, Lycium barbarum, and Cistanche deserticola in a mass ratio of 7:2:1, crush and sieve, and set aside. Weigh 1.0 g of cellulase and papain with an enzyme activity of 150,000 U / g in a mass ratio of 3:7, evenly disperse in 860 mL of water, and activate at 40°C for 10 min. Weigh 60 g of dried medicinal powder, mix evenly with the above complex enzyme solution, adjust the pH to 5.0, stir at 50°C for 2 h, and then inactivate the enzyme by ultrasound to obtain an enzymatic solution.

[0045] (2) Crude extraction: The enzymatic hydrolysis solution with the drug residue was stirred at 70°C for 2 h to obtain a crude extract.

[0046] (3) Refined extraction: Add 60 g of sunflower seed oil and 2 g of isopropyl myristate to the crude extract with the drug residue, stir and keep warm at 60°C for 2.5 h, filter out the drug residue, and obtain the refined extract.

[0047] (4) Emulsification: 0.4 g tocopherol, 0.1 g ascorbyl palmitate and 2.0 g γ-aminobutyric acid, as well as 77.5 g aqueous solution dissolved with 1 g disodium hydrogen phosphate and 0.2 g sodium dihydrogen phosphate were added to the extract, and the mixture was emulsified at 11000 r / min for 7 min in a homogenizer to obtain the polygonatum amino acid stock.

[0048] In the sunflower seed oil body, sterol accounts for 0.33% of its dry weight, and total protein accounts for 13.4% of its dry weight. The plant oil body of the present invention is prepared by a conventional water extraction method in the prior art, and the steps are as follows:

[0049] Plant seeds are soaked in water, then dispersed in a Tris-HCl buffer solution containing 0.4 mol / L sucrose and 0.5 mol / L sodium chloride, and stirred to obtain a homogenate. The seed residue is filtered out, and the upper layer of milk is collected by centrifugation at a speed of 10000 r / min for 30 minutes. The above dispersion and centrifugation operations are performed multiple times to obtain plant oil bodies.

[0050] Example 2

[0051] The only difference between this embodiment and embodiment 1 is that the crude extraction time is 1.5 h, and the feed liquid temperature is 65°C; the fine extraction time is 2.5 h, and the feed liquid temperature is 50°C.

[0052] Example 3

[0053] The only difference between this embodiment and embodiment 1 is that the crude extraction time is 3 hours, and the feed liquid temperature is 75°C; the fine extraction time is 4 hours, and the feed liquid temperature is 65°C.

[0054] Example 4

[0055] The only difference between this embodiment and embodiment 1 is that the amounts of some raw materials are as follows:

[0056] 800 mL (80 parts) of water, 80 g (8 parts) of sunflower oil, 5 g (0.5 parts) of oily esters, 114.5 g (11.45 parts) of stabilizer solution, of which disodium hydrogen phosphate and sodium dihydrogen phosphate remain unchanged in mass);

[0057] That is, the mass fraction of the oil phase increased from 6.2% to 8.5%.

[0058] Example 5

[0059] The only difference between this embodiment and embodiment 1 is that the amounts of some raw materials are as follows:

[0060] 880 mL (88 parts) of water, 40 g (4 parts) of sunflower oil, 1 g (0.1 parts) of oily esters, 78.5 g (7.85 parts) of stabilizer solution, of which disodium hydrogen phosphate and sodium dihydrogen phosphate remain unchanged in mass);

[0061] That is, the mass fraction of the oil phase dropped from 6.2% to 4.1%.

[0062] Example 6

[0063] The only difference between this example and Example 1 is that the sunflower seed oil bodies are replaced with an equal amount of soybean-corn oil bodies, and the preparation method is referred to Example 1. The soybean-corn oil bodies include soybean oil bodies and corn seed oil bodies in a mass ratio of 9:1, wherein sterols account for 0.33% of the dry weight thereof and total protein accounts for 11.6% of the dry weight thereof.

[0064] Example 7

[0065] The only difference between this embodiment and embodiment 1 is that in the emulsification step, the rotor speed of the homogenizing equipment is 8000 r / min and the emulsification time is 10 min.

[0066] Comparative Example 1

[0067] The only difference between this comparative example and Example 1 is that the dried medicinal material powder is not subjected to enzymatic hydrolysis but is stirred in an equal amount of 50° C. aqueous solution for 2 h.

[0068] Comparative Example 2

[0069] The only difference between this comparative example and Example 1 is that the enzyme inactivation method is boiling at 100° C. for 10 min.

[0070] Comparative Example 3

[0071] The only difference between this comparative example and Example 1 is that both the crude extraction and the fine extraction are carried out at 80°C.

[0072] Comparative Example 4

[0073] The only difference between this comparative example and Example 1 is that the sunflower seed oil bodies are replaced by an equal amount of rapeseed oil bodies, and the preparation method is referred to Example 1. In the rapeseed, sterols account for 0.50% of its dry weight, and total protein accounts for 7.5% of its dry weight.

[0074] Comparative Example 5

[0075] In this comparative example, only the aqueous phase was used to extract and add the amino acid additive under the conditions described in Example 1, without preparing an emulsion, and the amino acid puree of Polygonatum sibiricum was directly obtained.

[0076] The following tests were conducted on the polygonatum amino acid puree obtained from the above embodiments and comparative examples.

[0077] (1) Particle size and stability of Polygonatum amino acid emulsion

[0078] The particle size of the emulsion is an important indicator to describe its dispersibility. If the particle size of the emulsion is too large, the droplets will easily aggregate further, leading to demulsification.

[0079] The amino acid puree obtained in each embodiment and comparative example was diluted with a buffer solution with the same pH value and ion concentration to an equivalent and appropriate absorbance, and then the average particle size was measured by a Zetasizer laser particle size analyzer. The results are as follows: Figure 1 shown.

[0080] It can be seen that by changing the preparation conditions in accordance with the embodiments and comparative examples, the emulsion particle size is basically stable within a relatively low range; only in Comparative Example 4, rapeseed oil bodies are used as plant oil bodies, and the emulsion particle size is much higher than that of other experimental groups. This is because the rapeseed oil body has a low protein content and a poor emulsification effect.

[0081] Taking the amino acid puree obtained in Example 1 as an example, multiple stability tests such as standing, pH, particle concentration and heating were performed on it. The average particle size changes under different environmental changes are as follows: Figure 2 shown.

[0082] It can be seen that the polygonatum amino acid puree obtained in Example 1 has high stability during refrigerated storage. Changing the pH, increasing the ion concentration and heating will reduce the stability of the emulsion and need to be avoided during storage; this also illustrates the rationality of the process conditions of the method for preparing the polygonatum amino acid puree of the present invention.

[0083] (2) Polygonatum amino acid puree simulated digestion test

[0084] Simulated gastric fluid: Add 1 part of 0.15mmol / L calcium chloride and 16g / L pepsin solution to 4 parts of hydrochloric acid with a pH of 1.5 and set aside.

[0085] Simulated intestinal digestive fluid: Add 1 part of 6mmol / L calcium chloride, 6.25g / L bile salt and 3g / L pancreatic enzyme solution to 4 parts of sodium bicarbonate buffer with a pH of 7.5 and set aside.

[0086] The polysaccharide amino acid puree obtained in Example 1 and simulated gastric juice were mixed in a volume ratio of 1:1 and placed on one side of the semipermeable membrane in a container, and deionized water was placed on the other side. The container was placed in a shaking bed and a 37°C water bath to simulate gastric digestion for 3 hours, and then simulated intestinal digestive fluid with a volume twice its total volume was added to simulate small intestinal digestion for 3 hours; during this period, samples were taken on both sides of the semipermeable membrane every 30 minutes to measure the average particle size on the puree side and the total saponin concentration on the water side. The results are shown in FIG. Figure 3 shown.

[0087] It can be seen that during the simulated gastric juice digestion process, as the pH decreases, the surface charge of the emulsion decreases, and its particle size increases by dozens of times; while during the simulated intestinal digestive juice digestion process, as the pH increases and the fat is decomposed (and the stomach does not have enzymes to digest fat), the particle size gradually decreases, but is still higher than the initial particle size. On the other hand, the polygonatum amino acid puree obtained in Example 1 has an obvious sustained-release effect on saponins, and is mainly released during intestinal digestion.

[0088] (3) Yields of Polygonatum sibiricum polysaccharides, total saponins and flavonoids and percentage of sapogenins / total saponins

[0089] Using diosgenin standard as control, the vanillin colorimetric method was used to determine the content of total saponins in the Chinese medicinal materials (water-containing n-butanol extraction) used in the present invention and the polygonatum amino acid puree obtained in each embodiment and comparative example, and the yield was calculated; the HPLC method was used to determine the content of diosgenin in the polygonatum amino acid puree obtained in each embodiment of the present invention and comparative example, which represented its sapogenin content, and the percentage of sapogenin in the total saponins was calculated.

[0090] Using glucose standard as control, the phenol-sulfuric acid colorimetric method was used to determine the content of polysaccharide in the Chinese medicinal materials (boiling water extraction) used in the present invention and the polygonatum amino acid puree obtained in each embodiment and comparative example, and the yield was calculated.

[0091] Using rutin standard as control, sodium nitrite-aluminum nitrate colorimetry was used to determine the content of flavonoids in the Chinese medicinal materials used in the present invention (extracted with 60% ethanol aqueous solution) and the polygonatum amino acid puree obtained in each embodiment and comparative example, and the yield was calculated.

[0092] Table 1 Yields of polysaccharides, saponins and flavonoids and percentages of sapogenins / total saponins in Polygonatum amino acid puree:

[0093] Group Polysaccharide yield (%) Saponin yield (%) Flavonoid yield (%) Saponin / Total saponins (%) Example 1 84 94 89 2.4 Example 2 72 88 82 0.8 Example 3 79 77 84 4.8 Example 4 83 96 93 2.3 Example 5 84 91 88 1.8 Example 6 85 89 88 2.7 Example 7 85 92 90 2.2 Comparative Example 1 64 77 72 5.6 Comparative Example 2 86 91 84 12.4 Comparative Example 3 89 81 90 18.0 Comparative Example 4 84 98 90 2.1 Comparative Example 5 86 31 54 2.2

[0094] It can be seen from the above results that, compared with the optimal extraction methods of the three effective substances recorded in the literature, the extraction rates of polysaccharides, saponins and flavonoids in the preparation method of the polygonatum amino acid puree of the present invention are all above 70%, and in a better embodiment, they can even reach 80% or even above 90%; in addition, the sapogenin content in the polygonatum amino acid puree obtained by the present invention is relatively low in the total saponins, thereby maintaining the original biological activity of the saponins; finally, the plant oil bodies and oily esters added in the preparation method of the present invention greatly improve the extraction rates of saponins and flavonoids.

[0095] Obviously, the above embodiments are merely examples for clear explanation, and are not intended to limit the implementation methods. For those skilled in the art, other different forms of changes or modifications can be made based on the above description, and the obvious changes or modifications derived therefrom should still be considered as the protection scope of the present invention.

Claims

1. A polygonatum amino acid puree, characterized in that: The polygonatum amino acid puree is a stable emulsion with a pH value between 6.5 and 8.0 and an ion concentration of no more than 30 mmol / L in terms of sodium ions; the polygonatum amino acid puree comprises the following raw materials in parts by weight: 4 to 7 parts of Chinese medicinal materials, 80 to 88 parts of water, 4 to 8 parts of vegetable oil bodies, 0.1 to 0.5 parts of oily esters, 0.01 to 0.1 parts of antioxidants, 6 to 12 parts of stabilizers and 0.1 to 0.5 parts of amino acid additives.

2. The polygonatum amino acid puree according to claim 1, characterized in that: The polygonatum amino acid puree comprises the following raw materials in parts by weight: 6 parts of Chinese medicinal materials, 86 parts of water, 6 parts of vegetable oil bodies, 0.2 parts of oily esters, 0.05 parts of antioxidants, 7.75 parts of stabilizers and 0.2 parts of amino acid additives.

3. The polygonatum amino acid puree according to claim 1 or 2, characterized in that: In the plant oil body, sterol accounts for 0.2-1% of its dry weight, and oil body protein accounts for 10-15% of its dry weight.

4. The polygonatum amino acid puree according to claim 3, characterized in that: The oily esters are one or more of isopropyl myristate, ethyl oleate, and ethyl butyrate; The antioxidant is a combination of tocopherol and ascorbyl palmitate; The stabilizer is a phosphate buffer solution; The amino acid additive is gamma-aminobutyric acid.

5. The polygonatum amino acid puree according to claim 4, characterized in that: The Chinese medicinal materials, by mass, include 65-75% of polygonatum, 15-25% of wolfberry and 5-15% of cistanche; preferably, the Chinese medicinal materials, by mass, include 70% of polygonatum, 20% of wolfberry and 10% of cistanche.

6. A method for preparing the polygonatum amino acid puree according to any one of claims 1 to 5, characterized in that: The steps include: The Chinese medicinal materials are added with water, and the enzyme is killed after enzymolysis to obtain an enzymolysis liquid; The enzymatic hydrolysis liquid is heated and crudely extracted to obtain a crude extract; Adding plant oil and oily esters to the crude extract, heating for refined extraction, filtering out the drug residue, and obtaining a refined extract; Antioxidants, stabilizers and amino acid additives are added to the extract, and the extract is emulsified to obtain the polygonatum amino acid puree.

7. The method according to claim 6, characterized in that The enzymolysis uses 2000-5000 U / g of composite enzyme, including 25-35% by mass of cellulase, and the remainder is papain; the enzyme inactivation method is ultrasonic enzyme inactivation.

8. The method according to claim 6, characterized in that The crude extraction time is 1.5 to 3 hours, and the feed liquid temperature is 65 to 75°C; the fine extraction time is 2.5 to 4 hours, and the feed liquid temperature is 50 to 65°C; preferably, the crude extraction time is 2 hours, and the feed liquid temperature is 70°C; the fine extraction time is 3 hours, and the feed liquid temperature is 60°C.

9. The method according to claim 6, characterized in that The emulsification is carried out in a homogenizing device, the rotor speed of which is 8000-12000 r / min, and the emulsification time is 5-10 min; preferably, the rotor speed of the homogenizing device is 11000 r / min, and the emulsification time is 7 min.

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