Traditional Chinese medicine composition for promoting blood circulation to remove blood stasis and inducing diuresis to alleviate edema and preparation method thereof

Abstract

The invention belongs to the technical field of traditional Chinese medicines, and provides a traditional Chinese medicine composition for promoting blood circulation to remove blood stasis and inducing diuresis to alleviate edema and a preparation method thereof. The traditional Chinese medicine composition is prepared from the following components in parts by weight: 25-35 parts of radix angelicae sinensis, 10-15 parts of rhizoma chuanxiong, 10-20 parts of radix paeoniae rubra, 25-35 parts of semen coicis, 10-20 parts of rhizoma atractylodis macrocephalae, 10-15 parts of rhizoma atractylodis, 15-25 parts of radix astragali, 10-20 parts of poria cocos, 10-15 parts of polyporus umbellatus, 10-15 parts of radix cyathulae, 10-15 parts of rhizoma alismatis and 15-25 parts of flos lonicerae. The raw materials are wide in source, all the medicines are used together to achieve the effects of promoting blood circulation to remove blood stasis and inducing diuresis to alleviate edema, the synergistic interaction effect of the medicines is fully achieved, and the traditional Chinese medicine composition has the positive treatment effect on diseases such as lymphedema, varicose vein with edema of lower limbs and deep vein thrombosis.

Description

Technical Field

[0001] The invention belongs to the technical field of traditional Chinese medicines, and relates to a traditional Chinese medicine composition for promoting blood circulation and removing blood stasis, promoting dampness and reducing swelling, and a preparation method and application thereof. Background Art

[0002] Lymphedema is not uncommon in clinical practice. Its main cause is damage to lymphatic vessels or lymph nodes, which leads to obstruction of lymphatic fluid return. Once normal lymphatic vessels lose their compensatory ability, protein-rich lymph fluid will accumulate in the body, leading to lymphedema. There are non-surgical and surgical methods for the treatment of lymphedema. Non-surgical methods include: manual drainage, baking and binding therapy, pressure therapy, functional exercise, skin care, drug therapy, etc. Although diuretics can reduce swelling in the short term, long-term use can lead to complications such as hypotension and electrolyte disorders. It is not recommended to use benzopyrone and coumarin, which can promote macrophages to decompose protein and gradually improve limb edema. It has been reported that after 6 months of treatment of lymphedema with benzopyrone drugs, edema can be relieved to a certain extent. It is currently generally used as an auxiliary drug for the treatment of limb lymphedema.

[0003] Deep vein thrombosis of the lower extremities is an abnormal coagulation of blood in the veins, which completely or partially blocks the blood vessels. It is a venous reflux disorder and belongs to the categories of "edema", "blood stasis", "blood stasis syndrome" and "pulse arthritis" in traditional Chinese medicine. It is one of the common serious complications after major orthopedic surgery, especially in lower extremity surgery or trauma. The detachment of deep vein thrombosis emboli in the lower extremities can cause pulmonary embolism and be fatal. In the later stage, deep vein thrombosis syndrome in the lower extremities will occur, which will cause lower extremity pain, swelling, superficial varicose veins, skin changes (pigmentation, eczema, sclerosis), etc. In severe cases, venous ulcers will occur.

[0004] How to find a drug that can effectively treat lymphedema and deep vein thrombosis of the lower limbs is an urgent problem that we need to solve. Summary of the invention

[0005] In view of this, the present invention provides a Chinese medicine composition for promoting blood circulation and removing blood stasis, promoting dampness and reducing swelling, which has a positive therapeutic effect on diseases such as lymphedema, varicose veins of the lower limbs with edema, and deep vein thrombosis.

[0006] The Chinese medicinal composition for promoting blood circulation and removing blood stasis, promoting dampness and reducing swelling is prepared from the following components by weight: 25-35 parts of angelica sinensis, 10-15 parts of chuanxiong, 10-20 parts of red peony root, 25-35 parts of coix seeds, 10-20 parts of atractylodes macrocephala, 10-15 parts of atractylodes, 15-25 parts of astragalus, 10-20 parts of tuckahoe, 10-15 parts of umbellate, 10-15 parts of cyathula, 10-15 parts of oriental rhizome and 15-25 parts of honeysuckle.

[0007] In one embodiment of the present invention, the Chinese medicine composition is made of the following components by weight: 30 parts of Angelica sinensis, 12 parts of Chuanxiong, 15 parts of Red Peony Root, 30 parts of Coix Seed, 15 parts of Atractylodes macrocephala, 12 parts of Atractylodes lancea, 20 parts of Astragalus, 15 parts of Poria, 12 parts of Polyporus, 12 parts of Cyathula, 12 parts of Alisma Orientalis and 20 parts of Honeysuckle.

[0008] In one embodiment of the present invention, the Chinese medicine composition is made of the following components by weight: 35 parts of Angelica sinensis, 10 parts of Chuanxiong, 20 parts of Red Peony Root, 25 parts of Coix Seed, 20 parts of Atractylodes macrocephala, 10 parts of Atractylodes lancea, 25 parts of Astragalus, 10 parts of Poria, 15 parts of Polyporus, 10 parts of Cyathula, 15 parts of Alisma Orientalis and 15 parts of Honeysuckle.

[0009] In one embodiment of the present invention, the Chinese medicine composition is made of the following components by weight: 25 parts of Angelica sinensis, 15 parts of Chuanxiong, 10 parts of Red Peony Root, 35 parts of Coix Seed, 10 parts of Atractylodes macrocephala, 15 parts of Atractylodes lancea, 25 parts of Astragalus, 20 parts of Poria, 10 parts of Polyporus, 15 parts of Cyathula, 10 parts of Alisma Orientalis and 25 parts of Honeysuckle.

[0010] The present invention also provides a method for preparing the Chinese medicine composition for promoting blood circulation and removing blood stasis, promoting dampness and reducing swelling, comprising the following steps: weighing each raw medicinal material, crushing, soaking in water for 1-2 hours, decocting twice, each time adding water 5-8 times the total amount of the medicinal material, decocting for 0.5-1.5 hours each time, and combining the decoctions.

[0011] Preferably, the method for preparing the Chinese medicine composition for promoting blood circulation, removing blood stasis, promoting dampness and reducing swelling comprises the following steps: weighing each raw medicinal material, crushing it, soaking it in water for 1.5 hours, decocting it twice, adding 5 times the amount of water of the total amount of the medicinal materials each time, decocting it for 1.2 hours each time, and combining the decoctions.

[0012] The present invention also provides application of the Chinese medicine composition for promoting blood circulation and removing blood stasis, promoting dampness and reducing swelling in preparing medicine for promoting blood circulation and removing blood stasis, promoting dampness and reducing swelling.

[0013] The present invention also provides application of the Chinese medicinal composition for promoting blood circulation and removing blood stasis, promoting dampness and reducing swelling in preparing a medicine for treating lymphedema.

[0014] The present invention also provides the use of the Chinese medicine composition for promoting blood circulation and removing blood stasis, promoting dampness and reducing swelling in the preparation of medicine for treating varicose veins with edema of lower limbs or deep vein thrombosis of lower limbs.

[0015] Furthermore, the lower extremity deep vein thrombosis is in the acute stage.

[0016] The medicinal properties and effects of each Chinese medicinal material in the present invention are as follows:

[0017] Angelica: spicy and bitter in taste, warm in nature, enters the heart, liver, and spleen meridians, replenishes blood and promotes blood circulation, regulates menstruation and relieves pain, moisturizes and lubricates the intestines; used for irregular menstruation, amenorrhea, dysmenorrhea, accumulation of masses, metrorrhagia, abdominal pain due to deficiency and cold, paralysis, numbness of the skin, dry intestines and difficulty in defecation, heaviness after dysentery, carbuncles, ulcers, and injuries from falls.

[0018] Chuanxiong: spicy, warm in nature, enters the liver and gallbladder meridians, promotes qi and relieves depression, removes wind and dampness, promotes blood circulation and relieves pain; used for wind-cold headaches and dizziness, flank pain and abdominal pain, cold arthralgia and muscle spasms, amenorrhea, dystocia, postpartum blood stasis and pain, carbuncle and ulcer. Used for irregular menstruation, amenorrhea and dysmenorrhea, abdominal pain due to lumps, stabbing pain in the chest and flanks, swelling and pain due to falls, headache, and rheumatic pain.

[0019] Red Peony Root: bitter in taste, slightly cold in nature, enters the Liver Channel, clears away heat and cools blood, promotes blood circulation and removes blood stasis; used for heat entering the blood, warm toxin causing rash, vomiting blood and epistaxis, red and swollen eyes, liver depression and flank pain, amenorrhea and dysmenorrhea, abdominal pain due to lumps and injuries, carbuncles and ulcers.

[0020] Job's tears: sweet and light in taste, cool in nature, enters the spleen, stomach, and lung meridians, promotes dampness and strengthens the spleen, relaxes muscles and removes numbness, clears away heat and discharges pus, detoxifies and disperses nodules; used for edema, athlete's foot, dribbling urination, diarrhea, rheumatic pain, muscle and vein spasms, flat warts, etc.

[0021] Atractylodes: bitter and sweet in taste, warm in nature, enters the liver and kidney meridians, strengthens the spleen and replenishes Qi, dries dampness and promotes diuresis. It is used for symptoms such as spleen deficiency, poor appetite, indigestion, diarrhea, edema, spontaneous sweating, and fetal movement disorder.

[0022] Atractylodes: pungent and bitter in taste, warm in nature, enters the spleen, stomach, and liver meridians, dries dampness and strengthens the spleen, dispels wind and cold, and improves eyesight; used for dampness blocking the middle burner, abdominal distension, diarrhea, edema, beriberi and paralysis, rheumatic arthralgia, colds, night blindness, and blurred vision.

[0023] Astragalus: sweet in taste, slightly warm in nature, enters the lung and spleen meridians, tonifies Qi and raises Yang, consolidates the exterior and stops sweating, promotes diuresis and reduces swelling, promotes fluid production and nourishes blood, relieves stagnation and arthritis, removes toxins and discharges pus, heals sores and promotes tissue regeneration; used for Qi deficiency and fatigue, poor appetite and loose stools, sinking of Qi in the middle, long-term diarrhea and prolapse of the anus, bloody stool and metrorrhagia, spontaneous sweating due to exterior deficiency, Qi deficiency and edema, internal heat and thirst, blood deficiency and sallow complexion, hemiplegia, pain and numbness of arthritis, carbuncles that are difficult to heal, and long-term sores that do not heal.

[0024] Poria: sweet and light in taste, neutral in nature, enters the heart, lung, spleen, and kidney meridians, promotes diuresis and eliminates dampness, benefits the spleen and stomach, calms the mind and soothes the nerves, enhances the body's immune function, and has anti-tumor and liver-protecting effects.

[0025] Poria: It is mild in nature, sweet in taste, and enters the spleen, kidney, lung, and bladder meridians. It can dissipate dampness, promote diuresis and reduce swelling. It is used for edema, dysuria, athlete's foot, diarrhea, stranguria, and leucorrhea.

[0026] Sichuan Achyranthes bidentata: sweet, slightly bitter in taste, neutral in nature, enters the liver and kidney meridians, dispels wind and dampness, promotes menstruation and activates blood circulation; used to treat rheumatic pain in the waist and knees, foot atrophy and muscle spasms, hematuria, hematuria, amenorrhea in women, abdominal masses, retained placenta, joint pain, foot atrophy and muscle spasms, hematuria and hematuria, and injuries from falls.

[0027] Alisma: sweet and light in taste, cold in nature, enters the kidney and bladder meridians, promotes diuresis and eliminates dampness, relieves heat, resolves turbidity and reduces fat; used for urinary problems, edema, diarrhea, oliguria, phlegm and fluid, dizziness, and painful stranguria caused by heat.

[0028] Honeysuckle: sweet in taste, cold in nature, enters the lung, heart, and stomach meridians, dispels wind-heat, and clears blood toxins; it is used for various febrile diseases, such as fever, rash, rashes, heat-toxic sores, sore throat, etc., and the effect is significant.

[0029] The raw materials of the invention are widely available, and the combination of various medicines can play the role of promoting blood circulation and removing blood stasis, promoting dampness and reducing swelling, giving full play to the synergistic effect of the medicinal flavors, and has a positive therapeutic effect on diseases such as lymphedema, varicose veins of the lower limbs with edema, and deep vein thrombosis.

[0030] Compared with the prior art, the present invention has the following advantages:

[0031] After the blood stasis model rats were treated with the Chinese medicinal composition for promoting blood circulation and removing blood stasis, promoting dampness and reducing swelling, the hematocrit value, plasma viscosity and whole blood viscosity value of the rats were significantly reduced, and the blood rheology indexes were significantly improved.

[0032] The Chinese medicinal composition of the present invention significantly inhibits the ear swelling of mice caused by xylene and has a significant detumescence effect.

[0033] The Chinese medicine composition of the present invention can significantly alleviate the symptoms of lymphedema.

[0034] After the deep vein thrombosis model rats were treated with the Chinese medicine composition for promoting blood circulation and removing blood stasis, promoting dampness and reducing swelling, the rats' APTT, TT and PT were significantly prolonged, which has a positive therapeutic effect on deep vein thrombosis.

[0035] The Chinese medicinal prescription of the invention has mild medicinal properties, good curative effect and is suitable for clinical popularization and application. BRIEF DESCRIPTION OF THE DRAWINGS

[0036] Figure 1 Comparison of hematocrit among rats in each group. Different lowercase letters above the bar graph indicate significant differences among different groups (P<0.05).

[0037] Figure 2 Comparison of plasma viscosity among rats in each group. Different lowercase letters above the bar graph indicate significant differences among different groups (P<0.05).

[0038] Figure 3 Comparison of low shear rate among rats in each group. Different lowercase letters above the bar graph indicate significant differences among different groups (P<0.05).

[0039] Figure 4Comparison of high shear rate among rats in each group. Different lowercase letters above the bar graph indicate significant differences among different groups (P<0.05).

[0040] Figure 5 The ear swelling of mice in each group was compared. Different lowercase letters above the bar graph indicate significant differences among different groups (P<0.05).

[0041] Figure 6 Comparison of tail diameters of mice in each group. Different lowercase letters above the bar graph indicate significant differences among different groups (P<0.05).

[0042] Figure 7 Comparison of APTT of rats in each group. Different lowercase letters above the bar graph indicate significant differences among different groups (P<0.05).

[0043] Figure 8 Comparison of TT of rats in each group. Different lowercase letters above the bar graph indicate significant differences among different groups (P<0.05).

[0044] Fig. 9 Comparison of PT among rats in each group. Different lowercase letters above the bar graph indicate significant differences among different groups (P<0.05). DETAILED DESCRIPTION

[0045] In order to verify the efficacy of the Chinese medicine composition of the present invention, the inventors conducted a pharmacodynamics test study. It should be noted that the drugs selected for the pharmacodynamics test study of the present invention are drugs obtained by the representative formula of the present invention and its preparation method. Due to space limitations, the tests and results involved in the drugs obtained by other formulas and preparation methods included in the present invention are not listed here one by one.

[0046] Unless otherwise indicated, all technical and scientific terms used herein have the same meanings as those generally understood by those skilled in the art. Although the present invention describes only preferred methods and materials, any methods and materials similar or equivalent to those described herein may also be used in the implementation or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials associated with the documents. In the event of a conflict with any incorporated document, the content of this specification shall prevail.

[0047] Example 1

[0048] Angelica 30g, Chuanxiong 12g, Red Peony 15g, Coix Seed 30g, Atractylodes 15g, Atractylodes 12g, Astragalus 20g, Poria 15g, Polyporus 12g, Cyathula 12g, Alisma 12g and Honeysuckle 20g.

[0049] The preparation method is as follows: weigh each raw medicinal material, crush it, soak it in water for 1.5 hours, decoct it twice, add 5 times the amount of water to the total amount of the medicinal material each time, decoct it for 1.2 hours each time, and combine the decoctions.

[0050] Example 2

[0051] Angelica 35g, Chuanxiong 10g, Red Peony 20g, Coix Seed 25g, Atractylodes 20g, Atractylodes 10g, Astragalus 25g, Poria 10g, Polyporus 15g, Cyathula 10g, Alisma 15g and Honeysuckle 15g.

[0052] The preparation method is as follows: weigh each raw medicinal material, crush it, soak it in water for 2 hours, decoct it twice, add 8 times the amount of water to the total amount of the medicinal material each time, decoct it for 1.5 hours each time, and combine the decoctions.

[0053] Example 3

[0054] Angelica 25g, Chuanxiong 15g, Red Peony Root 10g, Coix Seed 35g, Atractylodes 10g, Atractylodes 15g, Astragalus 25g, Poria 20g, Polyporus 10g, Cyathula 15g, Alisma Orientalis 10g and Honeysuckle 25g.

[0055] The preparation method is as follows: weigh each raw medicinal material, crush it, soak it in water for 1.5 hours, decoct it twice, add 5 times the amount of water to the total amount of the medicinal material each time, decoct it for 1.2 hours each time, and combine the decoctions.

[0056] Example 4

[0057] Angelica 30g, Chuanxiong 15g, Red Peony Root 10g, Coix Seed 35g, Atractylodes 15g, Atractylodes 12g, Astragalus 25g, Poria 10g, Polyporus 15g, Cyathula 10g, Alisma Orientalis 12g and Honeysuckle 20g.

[0058] The preparation method is as follows: weigh each raw medicinal material, crush it, soak it in water for 2 hours, decoct it twice, add water 8 times the total amount of the medicinal materials each time, decoct it for 1 hour each time, and combine the decoctions.

[0059] Example 5

[0060] Angelica 25g, Chuanxiong 12g, Red Peony Root 15g, Coix Seed 30g, Atractylodes 10g, Atractylodes 10g, Astragalus 20g, Poria 15g, Polyporus 12g, Cyathula 15g, Alisma Orientalis 15g and Honeysuckle 25g.

[0061] The preparation method is as follows: weigh each raw medicinal material, crush it, soak it in water for 1.5 hours, decoct it twice, add 5 times the amount of water to the total amount of the medicinal material each time, decoct it for 0.5 hours each time, and combine the decoctions.

[0062] Example 6

[0063] Angelica 35g, Chuanxiong 10g, Red Peony Root 20g, Coix Seed 25g, Atractylodes 20g, Atractylodes 15g, Astragalus 25g, Poria 20g, Polyporus 10g, Cyathula 12g, Alisma Orientalis 10g and Honeysuckle 15g.

[0064] The preparation method is as follows: weigh each raw medicinal material, crush it, soak it in water for 1 hour, decoct it twice, add water 6 times the total amount of medicinal materials each time, decoct it for 1 hour each time, and combine the decoctions.

[0065] Example 7

[0066] Angelica 25g, Chuanxiong 10g, Red Peony Root 10g, Coix Seed 35g, Atractylodes 15g, Atractylodes 12g, Astragalus 15g, Poria 20g, Polyporus 15g, Cyathula 15g, Alisma Orientalis 12g and Honeysuckle 25g.

[0067] The preparation method is as follows: weigh each raw medicinal material, crush it, soak it in water for 1.5 hours, decoct it twice, add 5 times the amount of water to the total amount of the medicinal material each time, decoct it for 0.5 hours each time, and combine the decoctions.

[0068] Comparative Example 1

[0069] Chuanxiong 27g, red peony root 30g, coix seed 30g, white atractylodes 15g, atractylodes 12g, astragalus 20g, poria 15g, umbellate 12g, chuanxiong 12g, oriental water plantain 12g and honeysuckle 20g.

[0070] Compared with Example 1, the difference is that Angelica sinensis is not contained, and the dosage of Chuanxiong and Red Peony Root is increased.

[0071] The preparation method is similar to that of Example 1.

[0072] Comparative Example 2

[0073] Angelica 30g, Chuanxiong 12g, Red Peony Root 15g, Atractylodes 30g, Atractylodes 27g, Astragalus 20g, Poria 15g, Polyporus 12g, Cyathula 12g, Alisma 12g and Honeysuckle 20g.

[0074] Compared with Example 1, the difference is that coix seed is not contained, and the dosage of white atractylodes and atractylodes is increased.

[0075] The preparation method is similar to that of Example 1.

[0076] Comparative Example 3

[0077] Angelica 30g, Chuanxiong 12g, Red Peony Root 15g, Coix Seed 30g, Atractylodes 15g, Atractylodes 12g, Poria 25g, Polyporus 22g, Cyathula 12g, Alisma Orientalis 12g and Honeysuckle 20g.

[0078] Compared with Example 1, the difference is that Astragalus is not contained, and the dosage of Poria cocos and Polyporus umbellatus is increased.

[0079] The preparation method is similar to that of Example 1.

[0080] Comparative Example 4

[0081] Angelica 30g, Chuanxiong 12g, Red Peony Root 15g, Coix Seed 30g, Atractylodes 15g, Atractylodes 12g, Astragalus 20g, Poria 15g, Polyporus 12g, Alisma Orientalis 18g and Honeysuckle 26g.

[0082] Compared with Example 1, the difference is that Rhizoma Cynanchum is not contained, and the dosage of Rhizoma Alismatis and Flos Lonicerae is increased.

[0083] The preparation method is similar to that of Example 1.

[0084] Comparative Example 5

[0085] Angelica 20g, Chuanxiong 20g, Red Peony 8g, Coix Seed 20g, Atractylodes 22g, Atractylodes 8g, Astragalus 10g, Poria 25g, Polyporus 8g, Cyathula 18g, Alisma 8g and Honeysuckle 10g.

[0086] Compared with Example 1, the difference is that the proportions of the components are different.

[0087] The preparation method is similar to that of Example 1.

[0088] Efficacy Example 1 Experimental study of the Chinese medicine composition of the present invention on blood stasis model

[0089] 1. Establishment of blood stasis model in rats

[0090] 80 SPF SD rats, weighing 180-220g, were randomly selected as blank control groups, and the remaining rats were divided into 9 groups according to the random number method, 8 rats in each group, namely model control group, Example 1 group (given 8g / kg of the decoction of Example 1), Example 2 group (given 8g / kg of the decoction of Example 2), Example 3 group (given 8g / kg of the decoction of Example 3), Comparative Example 1 group (given 8g / kg of the decoction of Comparative Example 1), Comparative Example 2 group (given 8g / kg of the decoction of Comparative Example 2), Comparative Example 3 group (given 8g / kg of the decoction of Comparative Example 3), Comparative Example 4 group (given 8g / kg of the decoction of Comparative Example 4), and Comparative Example 5 group (given 8g / kg of the decoction of Comparative Example 5).

[0091] Each group was given the drug by gavage. Rats in the blank control group and the model control group were given an equal volume of saline by gavage, with a dose of 10 mL / kg, once a day for 7 consecutive days. On the 7th day, adrenaline 0.08 mL / 100 g body weight was injected subcutaneously twice, with an interval of 4 hours, and the rats were immersed in ice water for 5 minutes between the two injections.

[0092] 2. Index detection

[0093] The animals were not fed after treatment, and blood was drawn 1 hour after administration the next morning to measure hematocrit, whole blood viscosity, and plasma viscosity.

[0094] 3. Statistical methods

[0095] Graphpad Prism 9.0 software was used for data processing, and the measured data were expressed as mean ± standard deviation. One-way analysis of variance was used for comparison among multiple groups, and SNK-q was used for pairwise comparison among groups. The difference was considered statistically significant when P < 0.05.

[0096] 4. Experimental Results

[0097] Compared with the blank control group, the whole blood viscosity and plasma viscosity of the rats in the model control group increased, and the hematocrit value increased, indicating that the model was successfully established.

[0098] Compared with the model control group, the hematocrit values ​​of rats in each group of the embodiment were reduced. Compared with the comparative example 1-5 groups, the hematocrit values ​​of rats in each group of the embodiment were significantly reduced. Figure 1 .

[0099] Compared with the model control group, the plasma viscosity and whole blood viscosity values ​​of rats in each group of the embodiment were reduced. Compared with the comparative examples 1-5, the plasma viscosity and whole blood viscosity values ​​of rats in each group of the embodiment were significantly reduced. Figure 2-Figure 4 .

[0100] After the blood stasis model rats were treated with the Chinese medicinal composition for promoting blood circulation and removing blood stasis, promoting dampness and reducing swelling, the hematocrit value, plasma viscosity and whole blood viscosity value of the rats were significantly reduced, and the blood rheology indexes were significantly improved.

[0101] Efficacy Example 2 Experimental study on the effect of the Chinese medicine composition of the present invention on ear swelling in mice

[0102] 1. Test methods

[0103] 72 SPF grade Kunming mice, weighing 18-20g. The mice were randomly divided into a model control group, Example 1 group (administered 12g / kg of Example 1 decoction), Example 2 group (administered 12g / kg of Example 2 decoction), Example 3 group (administered 12g / kg of Example 3 decoction), Comparative Example 1 group (administered 12g / kg of Comparative Example 1 decoction), Comparative Example 2 group (administered 12g / kg of Comparative Example 2 decoction), Comparative Example 3 group (administered 12g / kg of Comparative Example 3 decoction), Comparative Example 4 group (administered 12g / kg of Comparative Example 4 decoction), Comparative Example 5 group (administered 12g / kg of Comparative Example 5 decoction), 8 mice in each group. Each group of mice was intragastrically administered, and each mouse in the model control group was intragastrically administered with 0.2mL of normal saline each time, and the administration was continued for 7 days, once a day.

[0104] 2. Index detection

[0105] One hour after the last administration, 0.03 mL of xylene was applied to the right auricle of each mouse. The mice were killed 0.5 hours later, and the left and right auricles were punched out with a stainless steel punch with a diameter of 8 mm, weighed, and the swelling rate was calculated.

[0106] Ear swelling rate (%) = (right ear weight - left ear weight) / left ear weight × 100%.

[0107] 3. Statistical methods

[0108] Graphpad Prism 9.0 software was used for data processing, and the measured data were expressed as mean ± standard deviation. One-way analysis of variance was used for comparison among multiple groups, and SNK-q was used for pairwise comparison among groups. The difference was considered statistically significant when P < 0.05.

[0109] 4. Experimental Results

[0110] Compared with the model control group, each group of the embodiment significantly inhibited the ear swelling of mice caused by xylene. Compared with the comparative examples 1-5, each group of the embodiment had significant differences in inhibiting the ear swelling of mice caused by xylene. Figure 5 .

[0111] The Chinese medicinal composition of the present invention significantly inhibits the ear swelling of mice caused by xylene and has a significant detumescence effect.

[0112] Efficacy Example 3 Experimental study on the effect of the Chinese medicine composition of the present invention on lymphedema in mice

[0113] 1. Test methods

[0114] 18 SPF Kunming mice, weighing 18-20 g, were randomly divided into a blank control group and a modeling group according to a random number table.

[0115] The mice in the modeling group were anesthetized by intraperitoneal injection of 0.02 mL / g of 10% chloral hydrate, and the tail was disinfected with 75% ethanol. A circular incision was made 2 cm from the base of the tail, and the skin of the tail at the incision was about 2 mm deep. The skin and subcutaneous tissue of the tail were removed at the same time. Under a dissecting microscope, methylene blue injection was injected into the tip of the tail, and a color reaction was observed in the lymphatic vessels accompanying the skin excision site and the blood vessels on both sides of the tail. The exposed blue-stained lymphatic vessels were then removed with an electric knife, and the skin was fixed to the surrounding muscles with sutures. Erythromycin ointment was applied to the wound surface after surgery to prevent infection.

[0116] The mice with successful modeling were randomly divided into a model control group and an Example 1 group (administered with 12 g / kg of the decoction of Example 1), with 6 mice in each group. Each group of mice was intragastrically administered, and each mouse in the blank control group and the model control group was intragastrically administered with 0.2 mL of normal saline each time, for 14 consecutive days, once a day. The changes in edema in the tail of the mice were observed and recorded.

[0117] 2. Index detection

[0118] On the 14th day of administration, the tail diameter of the mice was measured.

[0119] 3. Statistical methods

[0120] Graphpad Prism 9.0 software was used for data processing, and the measured data were expressed as mean ± standard deviation. One-way analysis of variance was used for comparison among multiple groups, and SNK-q was used for pairwise comparison among groups. The difference was considered statistically significant when P < 0.05.

[0121] 4. Experimental Results

[0122] Compared with the blank control group, the tail diameter of mice in the model control group increased significantly. Compared with the model control group, the tail diameter of mice in Example 1 group decreased significantly. Figure 6 .

[0123] The Chinese medicine composition of the present invention can significantly alleviate the symptoms of lymphedema.

[0124] Efficacy Example 4 Experimental study of the Chinese medicine composition of the present invention on deep vein thrombosis model

[0125] 1. Establishment of rat deep vein thrombosis model

[0126] 32 SPF SD rats, weighing 200-220 g, were randomly selected from 8 blank control groups and divided into 3 groups according to the random number method, 8 rats in each group, namely the model control group, the Example 1 group (administered with 8 g / kg of the decoction of Example 1), and the Example 2 group (administered with 8 g / kg of the decoction of Example 2). The model control group and the drug administration group rats were modeled.

[0127] After the rats were weighed and given 1% sodium pentobarbital intraperitoneal anesthesia, the rats' lower limbs were fixed with hip and knee flexion, and a 1 cm incision was made on the outside of the groin to separate the subcutaneous tissue of the rats. After the femur was exposed, the two ends of the femur were clamped with sterile hemostatic forceps and rotated in opposite directions until a snapping sound was heard. The integrity of the rat femur was checked for structural damage, which was determined to be a successful model. After the incision was sutured, the rats were raised normally without the use of antibiotics.

[0128] Each group was gavaged 3 days before modeling and 7 days after modeling. Rats in the blank control group and model control group were gavaged with an equal volume of normal saline, with a dose of 10 mL / kg, once a day for 10 consecutive days.

[0129] 2. Index detection

[0130] On the 7th day after modeling, blood was collected from the eyeballs, and the activated partial thromboplastin time (APTT), prothrombin time (PT), and thrombin time (TT) were detected according to the kit method.

[0131] 3. Statistical methods

[0132] Graphpad Prism 9.0 software was used for data processing, and the measured data were expressed as mean ± standard deviation. One-way analysis of variance was used for comparison among multiple groups, and SNK-q was used for pairwise comparison among groups. The difference was considered statistically significant when P < 0.05.

[0133] 4. Experimental Results

[0134] Compared with the blank control group, the APTT, TT and PT of rats in the model control group were significantly shortened, indicating that the model was successfully established.

[0135] Compared with the model control group, the APTT, TT and PT of rats in Example 1 and Example 2 groups were significantly prolonged. Figure 7-9 .

[0136] After the deep vein thrombosis model rats were treated with the Chinese medicine composition for promoting blood circulation and removing blood stasis, promoting dampness and reducing swelling, the rats' APTT, TT and PT were significantly prolonged, which has a positive therapeutic effect on deep vein thrombosis.

[0137] The above embodiments are not any form of limitation to the present invention. For those skilled in the art, any modification, replacement, etc. made within the scope of the technical solution of the present invention based on the technical essence of the present invention still falls within the protection scope of the technical solution of the present invention.

Claims

1. A Chinese medicine composition for promoting blood circulation and removing blood stasis, promoting dampness and reducing swelling, characterized in that: The traditional Chinese medicine composition is prepared from the following components by weight: 25-35 parts of angelica sinensis, 10-15 parts of chuanxiong, 10-20 parts of red peony root, 25-35 parts of coix seeds, 10-20 parts of atractylodes macrocephala, 10-15 parts of atractylodes, 15-25 parts of astragalus, 10-20 parts of tuckahoe, 10-15 parts of umbellate, 10-15 parts of cyathula, 10-15 parts of oriental water plantain and 15-25 parts of honeysuckle.

2. The Chinese medicine composition for promoting blood circulation and removing blood stasis, promoting dampness and reducing swelling according to claim 1, characterized in that: The Chinese medicine composition is prepared from the following components by weight: 30 parts of angelica sinensis, 12 parts of chuanxiong, 15 parts of red peony root, 30 parts of coix seeds, 15 parts of atractylodes macrocephala, 12 parts of atractylodes, 20 parts of astragalus, 15 parts of tuckahoe, 12 parts of umbellate, 12 parts of cyathula, 12 parts of oriental water plantain and 20 parts of honeysuckle.

3. The Chinese medicine composition for promoting blood circulation and removing blood stasis, promoting dampness and reducing swelling according to claim 1, characterized in that: The Chinese medicine composition is prepared from the following components by weight: 35 parts of angelica sinensis, 10 parts of chuanxiong, 20 parts of red peony root, 25 parts of coix seeds, 20 parts of atractylodes macrocephala, 10 parts of atractylodes, 25 parts of astragalus, 10 parts of tuckahoe, 15 parts of umbellate, 10 parts of cyathula, 15 parts of oriental water plantain and 15 parts of honeysuckle.

4. The Chinese medicine composition for promoting blood circulation and removing blood stasis, promoting dampness and reducing swelling according to claim 1, characterized in that: The Chinese medicine composition is prepared from the following components by weight: 25 parts of angelica sinensis, 15 parts of chuanxiong, 10 parts of red peony root, 35 parts of coix seeds, 10 parts of atractylodes macrocephala, 15 parts of atractylodes, 25 parts of astragalus, 20 parts of tuckahoe, 10 parts of umbellate, 15 parts of cyathula, 10 parts of oriental water plantain and 25 parts of honeysuckle.

5. The method for preparing the Chinese medicine composition for promoting blood circulation and removing blood stasis, promoting dampness and reducing swelling according to any one of claims 1 to 4, characterized in that: The preparation method comprises the following steps: weighing each raw medicinal material, crushing, soaking in water for 1-2 hours, decocting twice, adding water 5-8 times of the total amount of the medicinal material each time, decocting for 0.5-1.5 hours each time, and combining the decoctions.

6. The method for preparing the Chinese medicine composition for promoting blood circulation and removing blood stasis, promoting dampness and reducing swelling according to claim 5, characterized in that: The preparation method comprises the following steps: weighing each raw medicinal material, crushing, soaking in water for 1.5 hours, decocting twice, adding water 5 times the total amount of the medicinal material each time, decocting for 1.2 hours each time, and combining the decoctions.

7. Use of the Chinese medicinal composition for promoting blood circulation and removing blood stasis, promoting dampness and reducing swelling according to any one of claims 1 to 4 in the preparation of a medicine for promoting blood circulation and removing blood stasis, promoting dampness and reducing swelling.

8. Use of the Chinese medicinal composition for promoting blood circulation and removing blood stasis, promoting dampness and reducing swelling according to any one of claims 1 to 4 in the preparation of a drug for treating lymphedema.

9. Use of the Chinese medicinal composition for promoting blood circulation and removing blood stasis, promoting dampness and reducing swelling according to any one of claims 1 to 4 in the preparation of a drug for treating varicose veins with edema of the lower extremities or deep vein thrombosis of the lower extremities.

10. The use according to claim 9, characterized in that The lower extremity deep vein thrombosis is in the acute stage.

Images