Traditional Chinese medicine composition for treating gastric ulcer as well as preparation method and application of traditional Chinese medicine composition

Abstract

The invention belongs to the field of traditional Chinese medicines, and discloses a traditional Chinese medicine composition for treating gastric ulcer, which is mainly prepared from the following components in parts by weight: 5 to 20 parts of radix paeoniae alba, 5 to 20 parts of rhizoma atractylodis macrocephalae, 5 to 20 parts of pericarpium citri reticulatae, 5 to 20 parts of radix bupleuri, 5 to 20 parts of poria cocos, 2 to 8 parts of liquorice root, 5 to 20 parts of malt, 5 to 20 parts of radix pseudostellariae, 5 to 20 parts of lily bulb, 2 to 10 parts of folium perillae, 5 to 20 parts of fructus aurantii and 2 to 8 parts of rhizoma zingiberis. The invention further discloses a preparation method of the traditional Chinese medicine composition for treating gastric ulcer and application of the traditional Chinese medicine composition in preparation of a medicine for treating gastric ulcer. According to the traditional Chinese medicine composition, all the medicines are combined to achieve the effects of warming middle-jiao, invigorating spleen and replenishing qi, focus on the pathogenesis of the spleen and stomach deficiency cold syndrome, repair mucosal lesion and promote ulcer healing, and the problems that most patients suffering from the spleen and stomach deficiency cold syndrome suffer from persistent non-healing and repeated attack are solved.

Description

Technical Field

[0001] The invention belongs to the field of traditional Chinese medicine, and in particular relates to a traditional Chinese medicine composition for treating gastric ulcer, and a preparation method and application thereof. Background Art

[0002] Gastric ulcer (GU) is a gastric mucosal injury caused by gastric acid, pepsin and other substances that digest the mucosa and then penetrate the muscular layer. Its occurrence is closely related to stress, smoking, drinking, non-steroidal anti-inflammatory drugs, Helicobacter pylori infection and genetic factors. The main clinical manifestations of GU are recurrent, rhythmic, regular upper and middle abdominal pain after meals, accompanied by symptoms such as acid reflux, belching, nausea, and lingering illness. At present, Western medicine treatment of GU is mainly based on chemical drugs, but there are many adverse reactions, easy recurrence of the disease, and poor patient compliance. Clinical studies have shown that traditional Chinese medicine plays an important role in the treatment of GU, with many advantages such as multiple targets, multiple levels, multiple pathways, and high safety. However, there are still problems such as the weak correlation between traditional efficacy and modern pharmacological activity mechanism and single evaluation indicators. In addition, since gastric ulcer (spleen and stomach deficiency and cold syndrome) has the characteristics of recurrence, diversity, persistence and difficulty in healing, the existing Chinese patent medicines for the treatment of spleen and stomach deficiency and cold syndrome gastric ulcer are difficult to meet the treatment needs of this disease. Therefore, the problem of prolonged and repeated attacks of most patients with spleen and stomach deficiency and cold syndrome gastric ulcer is difficult to solve, which seriously affects the patients' quality of life and work status. Summary of the invention

[0003] The technical problem to be solved by the present invention is to overcome the deficiencies and defects mentioned in the above background technology and provide a Chinese medicine composition for treating gastric ulcer and a preparation method and application thereof.

[0004] In order to solve the above technical problems, the technical solution proposed by the present invention is: A traditional Chinese medicine composition for treating gastric ulcer, mainly composed of the following components in parts by weight: 5-20 parts of white peony root, 5-20 parts of atractylodes macrocephala, 5-20 parts of dried tangerine peel, 5-20 parts of bupleurum, 5-20 parts of poria, 2-8 parts of liquorice, 5-20 parts of malt, 5-20 parts of pseudoginseng, 5-20 parts of lily, 2-10 parts of perilla leaves, 5-20 parts of aurantium, and 2-8 parts of dried ginger.

[0005] The above-mentioned traditional Chinese medicine composition for treating gastric ulcer is preferably mainly composed of the following components in parts by weight: 8-20 parts of white peony root, 8-15 parts of atractylodes, 8-15 parts of tangerine peel, 8-15 parts of bupleurum, 8-15 parts of poria, 4-8 parts of licorice, 8-15 parts of malt, 8-15 parts of pseudoginseng, 8-15 parts of lily, 4-10 parts of perilla leaves, 8-15 parts of bitter orange, and 4-8 parts of dried ginger.

[0006] The above-mentioned traditional Chinese medicine composition for treating gastric ulcer is preferably mainly composed of the following components in parts by weight: 10 parts of white peony root, 10 parts of atractylodes macrocephala, 10 parts of tangerine peel, 10 parts of bupleurum, 10 parts of poria, 6 parts of licorice, 10 parts of malt, 10 parts of pseudostellaria, 10 parts of lily, 5 parts of perilla leaves, 10 parts of aurantium, and 6 parts of dried ginger.

[0007] In the above-mentioned traditional Chinese medicine composition for treating gastric ulcer, preferably, the bupleurum is vinegar bupleurum, the malt is roasted malt, and the fructus aurantii is roasted fructus aurantii with bran.

[0008] The above-mentioned traditional Chinese medicine composition for treating gastric ulcer, preferably, the dosage form of the traditional Chinese medicine composition includes any one of decoction, paste, tablet, capsule, pill, granule, powder.

[0009] Based on a general inventive concept, the present invention also provides a method for preparing the above-mentioned Chinese medicine composition for treating gastric ulcer, comprising the following steps: The Chinese medicinal components in the formula are weighed, soaked, decocted, the filtrates are combined, then heated, concentrated, and cooled, thereby completing the preparation of the Chinese medicinal composition for treating gastric ulcer. In the above preparation method, preferably, the ingredients are first soaked in cold water for 20-40 minutes, and the decoction includes at least two times, the first decoction lasts for 30-50 minutes, and the second decoction lasts for 20-40 minutes.

[0010] In the above preparation method, preferably, the heating and concentration is performed to a concentrated solution concentration of 0.8-1.2 g / mL.

[0011] Based on a general inventive concept, the present invention also provides a use of the above-mentioned traditional Chinese medicine composition for treating gastric ulcer in the preparation of a medicine for treating gastric ulcer.

[0012] In the above application, preferably, the gastric ulcer includes a spleen and stomach deficiency-cold type gastric ulcer, that is, a spleen and stomach deficiency-cold syndrome according to TCM diagnosis.

[0013] Compared with the prior art, the present invention has the following beneficial effects: In the Chinese medicine composition used in the present invention, Radix Pseudostellariae is sweet in taste, can invigorate qi, strengthen the spleen and produce body fluid, dried ginger is very pungent and hot, mainly enters the middle jiao, has the effect of warming the middle and dispersing cold, the two medicines are together the monarch medicine, has the effect of warming the middle, strengthening the spleen and invigorating qi; Atractylodes macrocephala is sweet and bitter in taste, warm in nature, sweet and warm to invigorate qi, bitter and dry to invigorate the spleen, Atractylodes macrocephala is combined with Radix Pseudostellariae to enhance the effect of invigorating qi and strengthening spleen; Poria cocos is sweet and light, can invigorate spleen and eliminate dampness, Poria cocos is combined with Radix Pseudostellariae to enhance the effect of invigorating spleen; Bupleurum is pungent and bitter in taste, has the effect of raising yang qi, and is combined with Radix Pseudostellariae to invigorate qi and raise yang, the three are together the minister medicine, enhance the effect of invigorating spleen and raising yang; White Peony Root is bitter and sour, can nourish blood, soften liver and relieve pain , white peony root with licorice can relieve stomach pain; bitter, pungent and sour, can regulate qi and relieve stomachache, and citrus aurantium with bupleurum can regulate liver and spleen, and lift and lower qi; tangerine peel is bitter and pungent, warm in nature, and has the effect of regulating qi and strengthening spleen; roasted malt is sweet and flat in nature, can promote qi and invigorate spleen and appetite, and can be combined with white atractylodes and tangerine peel to enhance the effect of invigorating qi and invigorating spleen; perilla leaf is pungent and warm in nature, can promote qi and relieve stomachache; lily is sweet in taste, can regulate qi, clear the heart and calm the mind, and can protect stomach yin. The above six herbs are adjuvants, which can strengthen spleen and regulate qi, relieve stomachache; licorice can invigorate spleen and harmonize the middle, and harmonize the properties of the herbs, and is the guiding drug. The combination of all the herbs has the effect of warming the middle, strengthening spleen and invigorating qi, hitting the pathogenesis of spleen and stomach deficiency and cold syndrome, repairing mucosal damage, promoting ulcer healing, and solving the problem of prolonged and repeated attacks of most patients with spleen and stomach deficiency and cold syndrome gastric ulcer. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 It is a photo of the effect of each experimental group on the morphology of the gastric mucosa of GU: “→” indicates the ulcer site; Figure 2 HE staining was used to observe the effects of each experimental group on the pathological morphology of gastric mucosa in GU rats (HE, ×150): “→” indicates the desquamation of gastric mucosal epithelium, “a” indicates epithelial cell edema, and “b” indicates inflammatory cell infiltration; Figure 3 PAS staining was used to observe the effects of each experimental group on the content of gastric mucosal mucus in GU rats; Figure 4 is the protective effect of each experimental group on the gastric mucosa of GU rats observed by scanning electron microscopy (×3000); Figure 5 is the protective effect of each experimental group on the gastric mucosa of GU rats observed by transmission electron microscopy (×5000); Figure 6 is the effect of each experimental group on the expression of phosphorylated Raf, MEK1 / 2, and ERK1 proteins in the gastric mucosa of GU rats (150×, scale bar = 100 μm); Figure 7 is the effect of each experimental group on the expression of phosphorylated IkBβ, IKKβ, and NF-κB proteins in the gastric mucosa of GU rats (150×, scale bar = 100 μm).

[0015] Figure 1-7Middle: A. normal group; B. model group; C. rabeprazole group; D. low-dose group of Example 1; E. medium-dose group of Example 1; F. high-dose group of Example 1.

[0016] Figure 8 It is the ultra-high performance liquid chromatography combined with high-resolution mass spectrum of the traditional Chinese medicine composition: A. The spectrum of the traditional Chinese medicine composition in positive ion mode; B. The spectrum of the traditional Chinese medicine composition in negative ion mode; 1. Hesperidin; 2. Pachymic acid B; 3. 2-Amino-5-guanidino-pentanoic acid; 4. Bupleurum saponin A; 5. Paeoniflorin; 6. Naringin; 7. Paeoniflorin; 8. Pseudostellaria cyclotide B; 9. Rosmarinic acid; 10. Luteolin; 11. Atractylodes lactone I; 12. Liquorice root; 13. Bupleurum saponin D; 14. Atractylodes lactone III; 15. 6-Gingerol. DETAILED DESCRIPTION

[0017] In order to facilitate the understanding of the present invention, the present invention will be described more comprehensively and meticulously below in conjunction with the accompanying drawings and preferred embodiments of the present invention, but the protection scope of the present invention is not limited to the following specific embodiments.

[0018] Unless otherwise defined, all professional terms used below have the same meanings as those generally understood by those skilled in the art. The professional terms used herein are only for the purpose of describing specific embodiments and are not intended to limit the scope of protection of the present invention.

[0019] Unless otherwise specified, various raw materials, reagents, instruments and equipment used in the present invention can be purchased from the market or prepared by existing methods.

[0020] Embodiment 1: A traditional Chinese medicine composition for treating gastric ulcer, comprising 10 g of white peony root, 10 g of atractylodes macrocephala, 10 g of dried tangerine peel, 10 g of vinegar bupleurum, 10 g of poria, 6 g of liquorice, 10 g of stir-fried malt, 10 g of pseudostellaria, 10 g of lily, 5 g of perilla leaf, 10 g of stir-fried fructus aurantii with bran, and 6 g of dried ginger. The traditional Chinese medicine pieces were purchased from the First Affiliated Hospital of Hunan University of Chinese Medicine.

[0021] The dosage form of the Chinese medicine composition for treating gastric ulcer in this embodiment is a decoction, and its preparation method is as follows: weigh 10 g of white peony root, 10 g of white atractylodes, 10 g of dried tangerine peel, 10 g of vinegar bupleurum, 10 g of Poria, 6 g of liquorice, 10 g of stir-fried malt, 10 g of pseudostellaria, 10 g of lily, 5 g of perilla leaf, 10 g of stir-fried fructus aurantii with bran, and 6 g of dried ginger, first soak them in cold water for 30 minutes, then decoct them twice, the first decoction is 40 minutes, and the amount of water used for decoction is 8 times the weight of the Chinese medicine components; the second decoction is 30 minutes, and the amount of water used for decoction is 5 times the weight of the Chinese medicine components, filter out and combine the medicinal liquid, heat and concentrate to 1 g / mL, cool and store at 4°C for standby use.

[0022] Embodiment 2: In this example, a rat model of spleen and stomach deficiency-cold type gastric ulcer (GU) was established to verify the technical effect of the Chinese medicine composition for treating gastric ulcer in Example 1 in treating spleen and stomach deficiency-cold type GU disease.

[0023] 1. Materials and Methods 1. Experimental Animals A total of 72 male SPF SD rats weighing (180±20) g were selected and purchased from Hunan Slake Jingda Experimental Animal Co., Ltd. (Animal License No. ZS-202310100026). They were kept in the Animal Experiment Center of Hunan University of Chinese Medicine, with an indoor temperature of 24℃-26℃ and a humidity of 45%-55%. They were adaptively fed for 5 days before the formal experiment, with free access to food and water during the period, and the lighting and darkness were kept alternating for 12 h every day.

[0024] 2. Experimental drugs Example 1 Drug: The formulation and preparation method are shown in Example 1.

[0025] Senna was purchased from the First Affiliated Hospital of Hunan University of Chinese Medicine. The preparation method was as follows: 50 g of senna was added to 500 mL of distilled water, and the mixture was decocted twice as in Example 1, and then the residue was removed, the mixture was filtered out and the liquid was combined, and the mixture was concentrated to 1 g / mL by heating, cooled, and stored at 4°C for later use.

[0026] The positive control drug was rabeprazole sodium enteric-coated capsules (Jichuang Pharmaceutical Group Co., Ltd., batch number 2305155), which was dissolved in normal saline to prepare a suspension, and the dosage was 2.08 mg / kg.

[0027] The modeling drug was indomethacin (Yunpeng Pharmaceutical Group Co., Ltd., batch number 20221028), prepared in the same way as rabeprazole sodium enteric-coated capsules, with a dosage of 4 mg / kg, which was prepared and used immediately.

[0028] 3. Experimental methods Referring to the previous experimental methods of this research group (Establishment and evaluation of rat experimental model of spleen and stomach deficiency and cold type gastric ulcer syndrome [J]. Chinese Journal of Basic Medicine of Traditional Chinese Medicine, 2020, 26(04): 468-473), the "bitter cold purgation + fatigue and exhaustion" method combined with indomethacin was used to establish a spleen and stomach deficiency and cold type GU rat model. After 5 days of adaptive feeding, 72 SD rats were divided into a normal group, a model group, a positive drug group, and a low, medium, and high dose group of the drug in Example 1 according to the random number table method, with a total of 6 groups, 12 rats in each group. The normal group rats were given 1 mL / 100 g distilled water by gavage every day, without any other treatment, and the rats in the other groups were given 1 mL / 100 g senna leaf decoction (1 g crude drug / mL) by gavage. 2 hours after administration, the rats in the model group, the positive drug group, and each drug group of Example 1 were placed in a water tank filled with normal temperature water to swim, and the rats were immediately fished out when they were completely submerged in the water for 3 seconds, and this was continued for 7 days to establish a spleen and stomach deficiency and cold syndrome model. Starting from the 8th day, 4 mg / kg indomethacin suspension was given by gavage for 5 consecutive days to establish a GU disease model. 4 hours after GU modeling, the positive drug group and Example 1 were given low, medium, and high doses of 2.08 mg / kg, 5.57 g / kg, 11.14 g / kg, and 22.28 g / kg of drugs for gavage treatment, respectively, and the drug was administered for 4 consecutive days.

[0029] 2. Testing indicators and methods 1. Observation of gastric mucosal morphology in rats The differences in gastric mucosa among the rats in each group were observed with the naked eye, as well as whether the rats with spleen and stomach deficiency-cold GU had GU signs such as bleeding, ulcers, and erosion.

[0030] 2. Determination of ulcer index (UI) and therapeutic index (TI) of gastric mucosa in rats Gastric mucosal injury was scored according to the scoring method established by our research group in the early stage: normal mucosa was scored as 0 points, ulcer (bleeding) length not exceeding 1 mm was scored as 1 point, 1-2 mm was scored as 2 points, 2-3 mm was scored as 3 points, 3-4 mm was scored as 4 points, and more than 4 mm was scored as 5 points. UI was equal to the average injury score of rats in each group, TI / %=(1-treatment group UI / model group UI)×100%.

[0031] 3. Gastric mucosal blood flow detection After the administration, all rats were fasted for 24 h, and the gastric mucosal blood flow was measured after anesthesia according to the laser blood flow meter method reported by Xu Yunjiao et al. The specific method is as follows: After the rats were anesthetized, the abdomen was cut open to expose the gastric tissue, and a small incision of 1.0 cm was made in the anterior wall of the gastric pylorus. After the blood flow velocity was stable, the gastric mucosal blood flow in the gastric antrum of each group of rats was measured using a laser speckle blood flow imaging probe.

[0032] 4. ELISA method to detect serum inflammatory factors and oxidative stress levels in rats After anesthesia, blood was collected from the abdominal aorta into a vacuum tube and centrifuged at 3500 r / min for 5 min at 4°C to collect the supernatant. The ELISA kit instructions were used to analyze the expression levels of inflammatory factors such as TNF-α, IL-18, and IL-1β in serum and the content or activity of oxidative stress indicators such as CAT, GST, and SOD.

[0033] 5. HE staining of gastric tissue The stomach was removed and cut open along the greater curvature of the stomach, the stomach contents were cleaned, flattened, photographed, and the ulcer condition was recorded. Part of the gastric tissue was removed, fixed with 4% paraformaldehyde, embedded, and sectioned in paraffin, rinsed in xylene anhydrous ethanol and 75% ethanol in turn, dehydrated, and then rinsed with tap water. The tissue sections were pretreated for 1 min, then dyed in hematoxylin solution for 5 min, washed with tap water, differentiated with differentiation solution, washed with tap water, blued with blue solution, rinsed with running water, then dehydrated in 95% alcohol for 1 min, and stained in eosin solution for 15 s. The pathological changes of gastric tissue were observed under an optical microscope and photographed and recorded.

[0034] 6. PAS staining of gastric tissue The prepared tissue sections were placed in PAS staining solution B for 15 min, rinsed with tap water, rinsed with distilled water twice, and then placed in PAS staining solution A for 30 min, protected from light, rinsed with running water for 5 min, and then placed in PAS staining solution C for 30 s, washed with tap water, differentiated with hydrochloric acid aqueous solution, washed with tap water, blued with ammonia water, and rinsed with running water. Finally, dehydration and transparency were performed, and the sections were sealed with neutral gum. Gastric tissue sections were observed under an optical microscope, images were collected, and PAS staining results were statistically analyzed.

[0035] 7. Scanning electron microscopy observation 1 mm 3 After rinsing, the stomach tissues of both sides were fixed in electron microscope fixative at room temperature for 2 h, and then transferred to 4°C for storage. The fixed samples were rinsed with 0.1 mol / L phosphate buffer PBS (pH 7.4) for 3 times, 15 min each time, and then fixed with 0.1 mol / L PBS (pH7.4) 1% osmium acid at room temperature in the dark for 2 h. The tissues were placed in 30%, 50%, 70%, 80%, 90%, 95%, and 100% ethanol in turn, 15 min each time, and dehydrated with isoamyl acetate for 15 min. The stomach tissues were dried using a critical point dryer, and the dried stomach tissues were attached to the conductive carbon film double-sided tape, placed on the sample stage of the ion sputtering instrument for gold spraying for 30 s, and finally observed and collected images under a scanning electron microscope.

[0036] 8. Transmission electron microscopy observation The gastric tissue was immersed in 2.5% glutaraldehyde and fixed at 4°C for 2 h, rinsed three times with 100 mmol / L sodium dimethyl arsenate buffer, and then immersed in 1% osmium tetroxide and fixed again at 4°C for 2 h. After rinsing three times with distilled water, it was dehydrated with different proportions of alcohol and acetone, embedded in epoxy resin, and ultrathinly sectioned. After lead-uranium double staining, copper grids for gastric tissue sections were prepared, and ultramicroscopic images of gastric tissue were observed and collected under a transmission electron microscope.

[0037] 9. Immunofluorescence detection of gastric mucosal phosphorylated Raf, MEK1 / 2, ERK1, IkBβ, IKKβ, and NF-κB protein expression Immunofluorescence was used to detect the expression of phosphorylated Raf, MEK, ERK1, IkBβ, IKKβ, and NF-κB proteins in gastric mucosa. The specific method was as follows: the embedded gastric tissue wax block was dewaxed and sliced, and antigen repair was performed using EDTA antigen repair solution. After the slices were dried, a circle was drawn around the tissue with an immunohistochemistry pen, and then the slices were placed flat in a light-proof humid box, and 3% hydrogen peroxide solution was added and incubated at room temperature in the dark for 25 minutes to block endogenous peroxidase; the slices were then placed flat in a light-proof humid box, and fetal bovine serum was added and blocked at room temperature for 30 minutes; rabbit anti-phosphorylated Raf (Ser446) primary antibody (1:400), rabbit anti-phosphorylated MEK1 / 2 (Ser222) primary antibody (1:300), rabbit anti-phosphorylated ERK1 (Thr197 + The samples were incubated with rabbit anti-phospho-IkBβ (1:400), rabbit anti-phospho-IkBβ (1:300), rabbit anti-phospho-IKKβ (1:100), rabbit anti-phospho-NF-κB (Ser337) (1:400) at 4°C overnight. The primary antibodies were removed and the gastric tissue sections were rinsed. HRP-labeled goat anti-rabbit secondary antibody was added to the samples and incubated in the dark for 1 h at room temperature. The samples were treated with TSAPlus fluorescent three-label four-color staining kit to make the samples show red, green and yellow fluorescence. DAPI staining solution was added for counterstaining. After rinsing with PBS, the samples were sealed with anti-fluorescence quenching sealing agent. Six fields of view were randomly selected for each section. The expression of phosphorylated Raf, MEK, ERK1, IkBβ, IKKβ, and NF-κB proteins in gastric tissue was observed under an inverted fluorescence microscope at 100 times and photographed and recorded. Image Pro Plus 6.0 was used to analyze and calculate the mean fluorescence intensity to evaluate the effect of the drug in Example 1 on the expression level of each protein in the gastric mucosa of GU rats with spleen and stomach deficiency and coldness.

[0038] 10. Statistical processing SPSS 24.0 software was used to analyze the data. ±s, one-way analysis of variance was used for comparison among groups, and LSD method was used for multiple comparisons. P<0.05 indicated that the difference was statistically significant.

[0039] 3. Experimental Results 1. Effects on gastric mucosal morphology, UI and TI in rats Effects of each group on the morphology of GU gastric mucosa Figure 1 As shown by Figure 1 It can be seen that the gastric mucosa of the rats in the normal group was smooth and intact, with no damage to the folds, and no edema, erosion, bleeding or ulcers; the gastric mucosa of the rats in the model group was severely eroded, with multiple linear bleeding bands or massive bleeding spots, and thickened folds; compared with the model group, the degree of gastric ulcer in the rats in the rabeprazole group was significantly alleviated; compared with the model group, the gastric mucosa of the rats in each dose group of the drug in Example 1 was relatively intact, with fewer bleeding bands and bleeding spots, among which the high dose group had the most obvious effect on repairing the damaged gastric mucosa of the rats.

[0040] The effects of each group on rat gastric mucosal UI and TI are shown in Table 1.

[0041] Table 1 Effects of each group on UI and TI of gastric mucosa in rats ( +s, n=6)

[0042] Note: ① Compared with the normal group, P <0.01; ② Compared with the model group, P <0.01. (Same as Table 1-Table 6) The results in Table 1 show that the gastric mucosal ulcer index of rats in the model group was significantly higher than that in the normal group ( P <0.01); After the drug treatment in Example 1, the gastric mucosal ulcer index of rats was significantly reduced ( P <0.01). Further studies have shown that the healing indexes of the low, medium and high dose groups of the drug in Example 1 are 77.81%, 88.02% and 90.53% respectively. The above results show that the drug in Example 1 has a good effect in treating GU of spleen and stomach deficiency and cold type, and the high dose group has the most obvious effect.

[0043] 2. HE staining to observe the effects of each group on the pathological changes of gastric mucosa in GU rats HE staining was used to observe the effects of each group on the pathological changes of gastric mucosa in GU rats. Figure 2As shown, the results showed that the gastric mucosal structure of the rats in the normal group was intact, the glandular structure was neat, and no epithelial cell shedding, inflammatory cell infiltration and congestion points were found in the various structural layers of the mucosa; the gastric mucosal structure of the rats in the model group was incomplete, the glandular arrangement was disordered, the epithelial cells were shedding, a large number of inflammatory cells infiltrated in the submucosal layer, and vascular dilation and congestion were seen in the muscular layer; compared with the model group, the gastric mucosal structural damage of the rats in the rabeprazole group was significantly alleviated, the degree of glandular disorder was improved, and a small amount of inflammatory cell infiltration and reduced epithelial cell shedding were found; compared with the model group, the gastric mucosal structure of the rats in each dose group of the drug in Example 1 was relatively complete, the glandular arrangement was tight, and a small amount of epithelial cell shedding and inflammatory cell infiltration were found, among which the epithelial cells in the high dose group were almost not shedding, showing the most obvious effect.

[0044] 3. PAS staining to observe the effects of each group on gastric mucosal mucus secretion in GU rats The important protective layer of gastric mucosal epithelium is mucus polysaccharide in mucus, which appears purple-red after PAS staining. Therefore, PAS staining was used to detect the content of mucus polysaccharide on the surface of rat gastric mucosal cells, that is, the number of purple spots. PAS staining was used to observe the effects of each group on gastric mucosal mucus secretion in GU rats. Figure 3 As shown in Table 2, the results showed that the mucus layer of the gastric tissue of the rats in the normal group showed strong positive staining by PAS staining; a large number of epithelial cells in the rats in the model group fell off, the mucosal layer structure was severely damaged, the PAS positive expression basically disappeared, and the percentage of positive expression area was significantly reduced ( P <0.01); compared with the model group, the epithelial cell shedding in the rabeprazole group and each dose group of the drug in Example 1 was reduced, the mucosal structure was relatively intact, PAS staining showed a certain degree of positivity, and the percentage of positive expression area increased ( P <0.01).

[0045] Table 2 Statistical results of PAS staining of gastric tissue of GU rats in each group ( +s, n=3)

[0046] 4. Effects of each group on gastric mucosal blood flow in GU rats The effects of each group on the gastric mucosal blood flow of GU rats are shown in Table 3. The results showed that compared with the normal group, the gastric mucosal blood flow of GU rats in the model group was significantly reduced ( P <0.01); compared with the model group, the gastric mucosal blood flow in the rabeprazole group and each dose group of Example 1 was significantly increased, and the gastric mucosal blood flow in the low, medium and high dose groups of Example 1 gradually increased, among which the high dose group had the most obvious effect ( P <0.01).

[0047] Table 3 Effects of each group on gastric mucosal blood flow in GU rats ( +s, n=6)

[0048] 5. Effects of each group on serum oxidative stress indices in GU rats The effects of each group on serum oxidative stress indicators in GU rats are shown in Table 4. The results showed that compared with the normal group, the levels of CAT and SOD in the serum of rats in the model group were significantly reduced ( P ﹤0.01), GST content increased significantly ( P ﹤0.01); Compared with the model group, the CAT and SOD contents in the rabeprazole group and each dose group of the drug in Example 1 were significantly increased ( P ﹤0.01), GST content was significantly reduced ( P ﹤0.01). The research results also show that the CAT and SOD contents in the low, medium and high dose groups of Example 1 gradually increase, while the GST content gradually decreases.

[0049] Table 4 Effects of each group on the levels of serum oxidative stress indexes in GU rats ( +s, n=6)

[0050] 6. Effects of each group on the levels of serum inflammatory factors in GU rats The results in Table 5 show that compared with the normal group, the levels of inflammatory factors TNF-α, IL-18, and IL-1β in the serum of rats in the model group were significantly increased ( P ﹤0.01); Compared with the model group, the levels of TNF-α, IL-18, and IL-1β in the rabeprazole group and each dose group of the drug in Example 1 were significantly reduced, and the indicators of the low, medium, and high dose groups of the drug in Example 1 gradually decreased, with the high dose group having the most obvious effect ( P ﹤0.01).

[0051] Table 5 Effects of each experiment on the levels of serum inflammatory factors in GU rats ( +s, n=6)

[0052] 7. Scanning electron microscopy observation of the protective effect of each group on the gastric mucosa of GU rats like Figure 4The results of scanning electron microscopy showed that the gastric mucosal structure of the rats in the normal group was intact, the epithelial cells were intact in morphology without necrosis and shedding, and neatly arranged microvilli were visible on the cell surface; the gastric mucosal structure of the rats in the model group was destroyed, the epithelial cells were extensively necrotic and shedding, and were disorderly arranged, with no boundaries between cells, and fewer microvilli on the cell surface; the gastric mucosal structural damage of the rats in the rabeprazole group and the various dose groups of the drug in Example 1 was significantly alleviated, the mucosal epithelial cells were intact in morphology and arranged tightly, with less shedding and necrosis, and abundant and neatly arranged microvilli on the cell surface, among which the high dose group of the drug in Example 1 had the most obvious effect.

[0053] 8. Transmission electron microscopy observation of the protective effect of each group on the gastric mucosa of GU rats Figure 5 The results showed that the gastric mucosal epithelial cells of the rats in the normal group had intact structure, large and round nuclei, and intact mitochondrial structure; the gastric mucosal epithelial cells of the rats in the model group lacked structural integrity, mitochondrial swelling, membrane loss, infiltration of a large number of inflammatory cells and macrophages, and local glandular destruction; the mitochondrial damage of the rats in the rabeprazole group and each dose group of the drug in Example 1 was alleviated, the infiltration of inflammatory cells and macrophages was reduced, and the epithelial cell structure was restored to integrity, among which the high dose group of the drug in Example 1 had the most obvious effect.

[0054] 9. Effects of each group on the expression of phosphorylated Raf, MEK1 / 2, and ERK1 proteins in the gastric mucosa of GU rats Figure 6 The immunofluorescence staining pictures of the gastric mucosa of rats in each group are shown in Table 6. The blue fluorescence indicates DAPI staining, the red fluorescence indicates phosphorylated Raf protein expression, the green fluorescence indicates phosphorylated MEK1 / 2 protein expression, and the yellow fluorescence indicates phosphorylated ERK1 expression. The results in Table 6 show that compared with the normal group, the expression of Raf, MEK1 / 2, and ERK1 proteins in the gastric mucosa of rats in the model group was significantly increased ( P ﹤0.01); Compared with the model group, the expression levels of Raf, MEK1 / 2, and ERK1 proteins in the rabeprazole group and each dose group of the drug in Example 1 were significantly reduced ( P ﹤0.01). The above results suggest from the protein level that the drug in Example 1 can treat spleen and stomach deficiency and cold syndrome GU by regulating the MAPK signaling pathway.

[0055] Table 6 Effects of each group on the expression of phosphorylated Raf, MEK1 / 2, and ERK1 proteins in the gastric mucosa of GU rats ( +s, n=3)

[0056] 10. Effects of each group on the expression of phosphorylated IkBβ, IKKβ and NF-κB proteins in the gastric mucosa of GU rats Figure 7The immunofluorescence staining pictures of the gastric mucosa of rats in each group are shown in Table 7. The blue fluorescence indicates DAPI staining, the red fluorescence indicates the expression of phosphorylated IkBβ protein, the green fluorescence indicates the expression of phosphorylated IKKβ protein, and the yellow fluorescence indicates the expression of phosphorylated NF-κB. The results in Table 7 show that compared with the normal group, the expression of IkBβ, IKKβ, and p-NF-κB proteins in the gastric mucosa of rats in the model group was significantly increased ( P ﹤0.01); Compared with the model group, the expression levels of IkBβ, IKKβ, and p-NF-κB proteins in the rabeprazole group and each dose group of the drug in Example 1 were significantly reduced ( P ﹤0.01). The above results suggest from the protein level that the drug in Example 1 can treat spleen and stomach deficiency and cold syndrome GU by regulating the NF-κB signaling pathway.

[0057] Table 7 Effects of each group on the expression of phosphorylated IkBβ, IKKβ and NF-κB proteins in the gastric mucosa of GU rats ( +s, n=3)

[0058] The above results show that the expression of phosphorylated Raf, MEK1 / 2, ERK1, IkBβ, IKKβ, and NF-κB proteins in the gastric mucosa of GU rats with spleen and stomach deficiency and cold type is significantly increased compared with the normal group. After intervention with the drug in Example 1, the phosphorylation of all the above proteins can be reduced, indicating that the MAPK and NF-κB signaling pathways are involved in mediating the effect of the drug in Example 1 on alleviating gastric mucosal damage in GU rats with spleen and stomach deficiency and cold type, mediating the improvement of the inflammatory response and oxidative stress of the drug in Example 1 in GU rats with spleen and stomach deficiency and cold type, and mediating the effect of the drug in Example 1 on reversing the reduction of gastric mucosal blood flow in GU rats with spleen and stomach deficiency and cold type. On the other hand, it also reveals that the repair of damaged gastric mucosa in GU rats with spleen and stomach deficiency and cold type by the drug in Example 1 is related to the inhibition of activation of MAPK and NF-κB signaling pathways.

[0059] The above GU rat model of spleen and stomach deficiency and cold type was established by the "bitter cold diarrhea + fatigue and exhaustion + indomethacin" method. The results showed that the gastric mucosa of the modeled rats had edema, bleeding, erosion, ulcers and other injuries, epithelial cell necrosis and shedding, a large number of inflammatory cells infiltrated, and the blood flow of the gastric mucosa was reduced. The results of HE staining, PAS staining, scanning electron microscopy and transmission electron microscopy observations all showed that the GU rat model of spleen and stomach deficiency and cold type was successful. After the drug intervention of Example 1, the pathological damage of the gastric mucosal tissue of the rat was alleviated, the blood flow of the gastric mucosa increased, the levels of CAT and SOD in the serum increased, and the levels of GST, TNF-α, IL-18, and IL-1β decreased, among which the high-dose group of the drug in Example 1 was the most obvious. It shows that the Chinese medicine composition of the present invention can reduce the release of proinflammatory factors and enhance the activity of antioxidant enzymes, thereby inhibiting oxidative stress and inflammatory response, and improving gastric mucosal damage and blood circulation.

[0060] In summary, the Chinese medicine composition of the present invention has an obvious intervention effect on GU rats with spleen and stomach deficiency and coldness, reduces gastric mucosal structural damage and dysfunction in GU rats with spleen and stomach deficiency and coldness, and thus promotes ulcer healing.

[0061] Embodiment 3: This example performs quality control on the Chinese medicine composition in Example 1, and the specific method includes: (1) Weigh 10 g white peony root, 10 g atractylodes, 10 g dried orange peel, 10 g vinegar bupleurum, 10 g poria, 6 g liquorice, 10 g roasted malt, 10 g pseudostellaria, 10 g lily, 5 g perilla leaf, 10 g roasted aurantium, and 6 g dried ginger. Soak in cold water for 30 minutes and then boil twice. The first time, boil for 40 minutes, and the amount of water used is 8 times the weight of the Chinese medicine components. The second time, boil for 30 minutes, and the amount of water used is 5 times the weight of the Chinese medicine components. Filter out and combine the liquid.

[0062] (2) Take 20 mL of the drug solution in a centrifuge tube and centrifuge it at 12000 rpm for 10 min at 4°C; use a pipette to accurately draw 150 μL of the supernatant drug solution, add 500 μL (200 μL of methanol, 200 μL of acetonitrile, 100 μL of water) of the extract containing the isotope-labeled internal standard, centrifuge it at 12000 rpm for 10 min at 4°C, filter it at 0.22 μm and place it in an injection vial. Vanquish ultra-high performance liquid chromatography combined with Orbitrap Exploris 120 high-resolution mass spectrometry was used to control the quality of the traditional Chinese medicine composition. The mobile phase was: phase A: ultrapure water containing 0.01% acetic acid; phase B: acetonitrile + isopropanol (volume ratio 1:1). The mass spectrometer spray voltage was 3.8 kV in positive ion mode and 3.4 kV in negative ion mode. The sheath gas flow rate and auxiliary gas flow rate were 50 and 15 Arb respectively. The ion transfer tube temperature was 320 °C. The full scan resolution was 60000, the secondary mass spectrometry resolution was 15000, and the collision energy was 30 V.

[0063] The quality control results of Chinese medicine composition are as follows Figure 8 As shown in the figure, 15 main active ingredients in the Chinese medicine composition were identified by positive ion mode mass spectrometry and negative ion mode mass spectrometry, namely hesperidin, pachymic acid B, 2-amino-5-guanidino-pentanoic acid, saikosaponin A, paeoniflorin, naringin, paeoniflorin, Pseudostellariae cyclotide B, rosmarinic acid, luteolin, atractylodes lactone I, liquiritigenin, saikosaponin D, atractylodes lactone III, and 6-gingerol. Under the same conditions, the above 15 main active ingredients were identified in three repeated experiments.

[0064] The above results indicate that the Chinese medicine composition in Example 1 has good stability and controllable quality.

[0065] Embodiment 4: This embodiment is verified by clinical practice and shows that the Chinese medicine composition of the present invention can effectively treat gastric ulcer of spleen and stomach deficiency and cold type, eliminate gastric ulcer symptoms, improve the manifestation of spleen and stomach deficiency and cold syndrome, and at the same time, the Chinese medicine composition of the present invention can also significantly reduce the recurrence rate of ulcers in patients, reduce the incidence of adverse reactions in patients, and improve the compliance of patients with medication. The Chinese medicine composition of the present invention has a good therapeutic effect on the treatment of gastric ulcer of spleen and stomach deficiency and cold type, and has a good clinical promotion and application value.

[0066] Case 1: Wang, male, 45 years old, first visit on March 13, 2012. The patient complained of epigastric pain for three years, with intermittent attacks. Western medicine diagnosed it as gastric ulcer. The symptoms did not improve significantly after conventional Western medicine treatment, so he came to see a doctor. Now he has epigastric pain, no acid reflux, likes warmth and pressure, feels heavy in the limbs, is afraid of cold and has cold limbs, has a general appetite, has a bland mouth, has loose stools, and is sensitive to spicy and cold foods. The tongue is pale, the coating is white and slippery, and the pulse is slow and weak. The syndrome is spleen and stomach deficiency and cold, and the prescription is: white peony root 15 g, white atractylodes 15 g, dried orange peel 10 g, vinegar bupleurum 15 g, Poria 10 g, licorice 8 g, stir-fried malt 15 g, Pseudostellaria heterophylla 10 g, lily 15 g, perilla leaf 10 g, bran stir-fried Citrus aurantium 12 g, and dried ginger 8 g. 14 doses, decocted in water, 1 dose per day, taken twice. Follow-up one year later showed that all symptoms had healed and had not recurred.

[0067] Case 2: Deng, female, 42 years old, first visit on February 16, 2013. The patient had epigastric pain for more than a year, and was diagnosed with gastric ulcer by Western medicine. After combined Chinese and Western medicine treatment, the symptoms did not improve significantly, so she came to see a doctor. Symptoms include dull pain in the epigastric region, mental fatigue, poor appetite, abdominal distension, mild acid reflux, no heartburn, chills and cold limbs, and loose stools. The tongue is pale with tooth marks, the coating is thin and white, and the pulse is slow and weak. The Chinese medicine diagnosis is spleen and stomach deficiency and cold syndrome, and the prescription is: white peony root 10 g, white atractylodes 10 g, dried orange peel 10 g, vinegar bupleurum 10 g, Poria 10 g, licorice 6 g, stir-fried malt 10 g, Pseudostellaria heterophylla 10 g, lily 10 g, perilla leaf 5 g, bran stir-fried Citrus aurantium 10 g, and dried ginger 6 g. 21 doses, decocted in water, 1 dose per day, taken twice. When followed up after taking the medicine, the patient's epigastric pain had healed and his appetite was back to normal.

[0068] Case 3: Zhu, male, 40 years old, first visit on November 4, 2013. The patient complained of epigastric pain for half a year, and was diagnosed with gastric ulcer by Western medicine. After conventional Western medicine treatment, the symptoms did not improve significantly, so he came to see a doctor. Symptoms include epigastric pain, fatigue, cold limbs, loss of appetite, nausea and vomiting, clear water in vomitus, abdominal distension, loose stools, and even indigestion. The tongue is pale, the tongue coating is white and slippery, and the pulse is slow and weak. The syndrome is diagnosed as spleen and stomach deficiency and cold syndrome, and the prescription is: white peony root 15 g, white atractylodes 15 g, tangerine peel 12 g, vinegar bupleurum 15 g, Poria 15 g, licorice 8 g, stir-fried malt 15 g, Pseudostellaria heterophylla 15 g, lily 15 g, perilla leaf 8 g, bran stir-fried Citrus aurantium 12 g, and dried ginger 8 g. 14 doses, decocted in water, 1 dose per day, taken twice. Follow-up one year later showed that all symptoms had healed and had not recurred.

[0069] Case 4: Zhang, male, 55 years old, first visit on April 23, 2014. The patient complained of epigastric pain for two and a half years, Helicobacter pylori positive, and was diagnosed with gastric ulcer by Western medicine. He was given conventional Western medicine for more than a year, but the disease still recurred, so he came to see a doctor. He now has dull epigastric pain, which is continuous, acid reflux is not obvious, he does not want to eat, he likes warmth and pressure, his limbs are heavy, he is tired and weak, his limbs are not warm, his face is dull, and his mouth is tasteless. His tongue is pale with white and slippery coating, and his pulse is slow and weak. The syndrome is diagnosed as spleen and stomach deficiency and cold syndrome, and the prescription is: white peony root 15 g, white atractylodes 12 g, dried orange peel 10 g, vinegar bupleurum 12 g, Poria 10 g, licorice 8 g, stir-fried malt 10 g, Pseudostellaria heterophylla 10 g, lily 10 g, perilla leaf 8 g, stir-fried bitter orange 10 g, and dried ginger 6 g. 14 doses, decocted in water, 1 dose per day, taken twice. By taking antibiotics and other western medicines, the patient's Helicobacter pylori has been completely eradicated and he no longer has stomach pain. The patient said that taking Chinese medicine in combination with western medicine significantly reduced adverse reactions such as nausea and vomiting.

[0070] Case 5: Lin, female, 25 years old, first visit on December 21, 2014. The patient complained of epigastric pain for half a year, with intermittent attacks, which seriously affected her daily work and life. Western medicine diagnosed it as Helicobacter pylori-related gastric ulcer. After Western medicine treatment, the symptoms did not improve significantly, so she came to see a doctor. Now she has dull epigastric pain, which is continuous, aggravated by fatigue, loss of appetite, fatigue, abdominal distension, accompanied by nausea and vomiting, and clear sputum can be seen. The tongue is pale with teeth marks, and the pulse is deep, tight and weak. The syndrome is spleen and stomach deficiency and cold, prescription: white peony root 10 g, white atractylodes 10 g, dried tangerine peel 10 g, vinegar bupleurum 10 g, Poria 10 g, licorice 6 g, stir-fried malt 10 g, Pseudostellaria heterophylla 8 g, lily 8 g, perilla leaf 8 g, bran stir-fried Citrus aurantium 8 g, dried ginger 5 g. 14 doses, decocted in water, 1 dose per day, taken twice, and western medicine is taken at the same time. Follow-up one month later showed that the patient's ulcer had disappeared, Helicobacter pylori was negative, and no adverse reactions occurred during the medication period; follow-up one year later showed that the ulcer had not recurred.

[0071] Case 6: Li, female, 35 years old, first visit on November 12, 2015. The patient complained of epigastric pain for one year, which recurred repeatedly. Western medicine diagnosed it as gastric ulcer. After Western medicine treatment, the symptoms did not improve significantly, so she came to see a doctor. The current symptoms are epigastric pain, like warmth and pressure, no acid reflux, aggravated by negative emotions, abdominal distension, indigestion, weight loss, loose stools, and dull complexion. The tongue is pale with teeth marks, and the pulse is deep, tight and weak. The syndrome is diagnosed as spleen and stomach deficiency and cold syndrome. The prescription is: white peony 10 g, white atractylodes 10 g, tangerine peel 10 g, vinegar bupleurum 10 g, Poria 10 g, licorice 6 g, stir-fried malt 10 g, Pseudostellaria heterophylla 10 g, lily 10 g, perilla leaf 5 g, bran stir-fried Citrus aurantium 10 g, and dried ginger 6 g. 14 doses, decocted in water, 1 dose per day, taken twice. Follow-up after one year, all symptoms have been cured and have not recurred.

[0072] Case 7: Jiang, male, 42 years old, first visit on March 7, 2016. The patient complained of epigastric pain for one year, with recurrent attacks. Western medicine diagnosed it as gastric ulcer. He was given conventional Western medicine treatment, but there was no significant improvement, so he came to see a doctor. Now he has epigastric pain, likes warmth and pressure, cold limbs, little appetite, poor appetite, loose stools, and the symptoms are significantly aggravated after eating cold food. The tongue is pale with white and slippery coating, and the pulse is deep, tight and weak. The syndrome is diagnosed as spleen and stomach deficiency and cold syndrome, and the prescription is: white peony root 10 g, white atractylodes 10 g, tangerine peel 10 g, vinegar bupleurum 10 g, Poria 10 g, licorice 6 g, stir-fried malt 10 g, Pseudostellaria heterophylla 10 g, lily 10 g, perilla leaf 5 g, bran stir-fried Citrus aurantium 10 g, and dried ginger 6 g. 14 doses, decocted in water, 1 dose per day, taken twice. The patient came back for a follow-up visit on March 21, 2016. After syndrome differentiation, the original prescription was given for treatment. Follow-up one year later showed that all symptoms had been eliminated and there had been no recurrence.

[0073] Case 8: Chen, male, 38 years old, first visit on November 16, 2016. The patient complained of epigastric pain for more than two years, and was diagnosed with gastric ulcer by Western medicine. After combined treatment of Chinese and Western medicine, the disease still recurred and did not heal, seriously affecting daily work and life, so he came to see a doctor. Now he has cold pain in the epigastric region, no acid reflux and heartburn, fatigue, heaviness of limbs, loss of appetite, bland mouth without thirst, and loose stools. The tongue is pale with teeth marks, and the pulse is slow and weak. The syndrome is diagnosed as spleen and stomach deficiency and cold syndrome, and the prescription is: white peony 20 g, white atractylodes 15 g, tangerine peel 15 g, vinegar bupleurum 15 g, poria 10 g, licorice 8 g, stir-fried malt 15 g, Pseudostellaria heterophylla 15 g, lily 15 g, perilla leaf 10 g, bran stir-fried Citrus aurantium 15 g, and dried ginger 8 g. 21 doses, decocted in water, 1 dose per day, taken twice. Follow-up one year later showed that all symptoms had disappeared without recurrence.

[0074] Case 9: Zhou, female, 47 years old, first visit on February 20, 2017. The patient complained of epigastric pain for 1 and a half years, with intermittent attacks. Western medicine diagnosed it as gastric ulcer. After Western medicine treatment, it was still not cured, so she came to see a doctor. Now she has epigastric pain, likes warmth and pressure, cold limbs, dull complexion, abdominal distension, loss of appetite, loose stools, aversion to cold food, and easy anxiety. The tongue is pale with white and slippery coating, and the pulse is slow and weak. The syndrome is diagnosed as spleen and stomach deficiency and cold syndrome. The prescription is: white peony 15 g, white atractylodes 15 g, tangerine peel 15 g, vinegar bupleurum 15 g, Poria 10 g, licorice 8 g, stir-fried malt 10 g, Pseudostellaria heterophylla 15 g, lily 15 g, perilla leaf 10 g, bran stir-fried Citrus aurantium 15 g, and dried ginger 8 g. 21 doses, decocted in water, 1 dose per day, taken twice. Follow-up one year later showed that all symptoms had subsided and had not recurred.

[0075] Case 10: He, female, 33 years old, first visit on November 5, 2017. The patient complained of epigastric pain for two years, and was diagnosed with gastric ulcer by Western medicine. After conventional Western medicine treatment, the disease still recurred and did not heal, so she came to see a doctor. Epigastric pain is now seen, but there is no obvious acid reflux, fear of cold and cold limbs, pale complexion, poor appetite, abdominal distension with abdominal pain, loose stools, preference for warmth and dislike for cold. The tongue is pale with a thin white coating, and the pulse is slow and weak. The syndrome is diagnosed as spleen and stomach deficiency and cold syndrome, and the prescription is: white peony root 15 g, white atractylodes 15 g, dried orange peel 12 g, vinegar bupleurum 12 g, Poria 10 g, licorice 8 g, stir-fried malt 12 g, Pseudostellaria heterophylla 15 g, lily 15 g, perilla leaf 8 g, stir-fried bitter orange 12 g, and dried ginger 8 g. 21 doses, decocted in water, 1 dose per day, taken twice. Follow-up one year later showed that all symptoms were cured and there was no recurrence.

[0076] The technical solution of the present invention is described in detail above in combination with the embodiments / drawings, but the present invention is not limited to the above technical solution. For ordinary technicians in this technical field, after knowing the contents recorded in the present invention, they can make several equivalent changes and substitutions without departing from the principle of the present invention. These equivalent changes and substitutions should also be regarded as belonging to the protection scope of the present invention.

Claims

1. A Chinese medicine composition for treating gastric ulcer, characterized in that: The invention mainly consists of the following components in parts by weight: 5-20 parts of white peony root, 5-20 parts of atractylodes macrocephala, 5-20 parts of dried tangerine peel, 5-20 parts of bupleurum, 5-20 parts of poria, 2-8 parts of licorice, 5-20 parts of malt, 5-20 parts of pseudoginseng, 5-20 parts of lily, 2-10 parts of perilla leaf, 5-20 parts of aurantium, and 2-8 parts of dried ginger.

2. The Chinese medicine composition for treating gastric ulcer according to claim 1, characterized in that: The invention mainly consists of the following components in parts by weight: 8-20 parts of white peony root, 8-15 parts of atractylodes macrocephala, 8-15 parts of dried tangerine peel, 8-15 parts of bupleurum, 8-15 parts of poria, 4-8 parts of licorice, 8-15 parts of malt, 8-15 parts of pseudoginseng, 8-15 parts of lily, 4-10 parts of perilla leaf, 8-15 parts of fructus aurantii, and 4-8 parts of dried ginger.

3. The Chinese medicine composition for treating gastric ulcer according to claim 1, characterized in that: The invention mainly consists of the following components in parts by weight: 10 parts of white peony root, 10 parts of atractylodes macrocephala, 10 parts of dried tangerine peel, 10 parts of bupleurum root, 10 parts of poria, 6 parts of licorice, 10 parts of malt, 10 parts of pseudoginseng, 10 parts of lily, 5 parts of perilla leaf, 10 parts of aurantium and 6 parts of dried ginger.

4. The Chinese medicine composition for treating gastric ulcer according to any one of claims 1 to 3, characterized in that: The bupleurum root is vinegar bupleurum root, the malt is roasted malt, and the fructus aurantii is roasted fructus aurantii with bran.

5. The Chinese medicinal composition for treating gastric ulcer according to any one of claims 1 to 3, characterized in that: The dosage form of the Chinese medicine composition includes any one of decoction, paste, tablet, capsule, pill, granule and powder.

6. A method for preparing a Chinese medicine composition for treating gastric ulcer according to any one of claims 1 to 5, characterized in that: The following steps are involved: The Chinese medicinal components in the formula are weighed, soaked, decocted, the filtrates are combined, then heated, concentrated, and cooled, thereby completing the preparation of the Chinese medicinal composition for treating gastric ulcer.

7. The preparation method according to claim 6, characterized in that: First soak in cold water for 20-40 minutes, and the decoction includes at least two times, the first decoction is 30-50 minutes, and the second decoction is 20-40 minutes.

8. The preparation method according to claim 6, characterized in that: The heating and concentration is performed until the concentration of the concentrated solution is 0.8-1.2 g / mL.

9. Use of the traditional Chinese medicine composition for treating gastric ulcer according to any one of claims 1 to 5 in preparing a medicine for treating gastric ulcer.

10. The use according to claim 9, characterized in that The gastric ulcer includes gastric ulcer of spleen and stomach deficiency and cold type.

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