Compound gel for treating animal mastitis and preparation method thereof

Abstract

The invention provides a compound gel for treating animal mastitis and a preparation method thereof, and relates to the technical field of medicines, the compound gel is prepared by taking houttuynia cordata, honeysuckle and chymotrypsin in a specific ratio as main raw materials and adopting a specific process. Specifically, firstly, houttuynia cordata and honeysuckle are soaked so that effective components in the houttuynia cordata and the honeysuckle can be better extracted, then the effective components in the houttuynia cordata and the honeysuckle are extracted through distillation, then chymotrypsin, carbomer, a humectant, propylene glycol, a pH regulator and water are added, and the compound gel is obtained. The compound gel prepared by the method disclosed by the invention can be applied to a mammary inflammation area of an animal through a medicine patch of an ultrasonic medicine penetration instrument or directly applied to the mammary inflammation area of the animal without the first-pass effect of the liver, so that the damage of gastrointestinal tract digestive juice to the medicine is reduced, and the compound gel has a better curative effect on treating the animal mastitis.

Description

Technical Field

[0001] The invention relates to the technical field of medicines, and in particular to a compound gel for treating animal mastitis and a preparation method thereof. Background Art

[0002] Animal mastitis is one of the most common diseases that harm the animal breeding industry. This disease not only significantly reduces the milk production of animals, but also makes the production performance of animals worse and the quality of milk decreases. The pathogens that cause mastitis exist in the animal body or the surrounding environment. Poor sanitary conditions in the living environment and reduced immunity of animals increase the probability of infection by pathogens.

[0003] At present, the treatment of animal mastitis is mainly antibiotics. Although the use of antibiotics has achieved certain therapeutic effects, the long-term use of antibiotics not only increases the drug resistance of pathogenic microorganisms, but also causes serious excess of antibiotic residues in milk. Therefore, the use of traditional Chinese medicine and other non-antibiotics to treat dairy cow mastitis has become a new research direction. However, the traditional Chinese medicine used to treat animal mastitis is generally taken orally and injected, which is not very convenient to use, and the efficacy needs to be further improved. Summary of the invention

[0004] The problem solved by the invention is that the Chinese medicine for treating animal mastitis is generally taken orally or injected, which is not convenient to use and the efficacy needs to be further improved.

[0005] In order to solve the above problems, the present invention provides a method for preparing a compound gel for treating animal mastitis, comprising:

[0006] Step S1, moistening Houttuynia cordata and honeysuckle with water, and then soaking them in water;

[0007] Step S2, steam distilling the soaked Houttuynia cordata and honeysuckle, collecting the distillate to obtain a primary distillate; the ratio of the total mass of the Houttuynia cordata and honeysuckle to the mass of the primary distillate is 1:(0.9-1.1);

[0008] Step S3, redistillating the primary distillate, collecting the distillate to obtain a heavy distillate; the mass ratio of the heavy distillate to the primary distillate is 1:(4-6);

[0009] Step S4, adding chymotrypsin to the heavy distillate, stirring evenly, adding carbomer, mixing evenly, adding moisturizer and propylene glycol in sequence, stirring evenly, standing for a preset time, adding a pH regulator, adjusting the pH value to 6.8-7.2, adding water, stirring evenly, and obtaining a compound gel;

[0010] Among them, the amounts of raw materials used in the preparation process of the compound gel are as follows, by weight: 2000-3000 parts of Houttuynia cordata, 2000-3000 parts of honeysuckle, 2-5 parts of chymotrypsin, 20-40 parts of carbomer, 30-50 parts of moisturizer, and 20-30 parts of propylene glycol.

[0011] Optionally, in step S1, the soaking treatment time is 20-40 minutes.

[0012] Optionally, in step S1, the ratio of the total mass of the Houttuynia cordata and honeysuckle to the mass of water used for the soaking treatment is 1:(5-6).

[0013] Optionally, in step S4, the preset time is 3-5 hours.

[0014] Optionally, in step S4, the pH adjuster is triethanolamine.

[0015] Optionally, in step S4, the moisturizing agent is glycerin.

[0016] Optionally, in the step S4, the amount of the compound gel is 9500-10500 parts by weight.

[0017] Optionally, the water used in the preparation process of the composite gel is purified water.

[0018] Optionally, in step S2, the ratio of the total mass of the herb Houttuynia cordata and the flower honeysuckle to the mass of the primary distillate is 1:1.

[0019] The present invention also provides a compound gel for treating animal mastitis, which is prepared by the preparation method of the compound gel for treating animal mastitis as described above.

[0020] Compared with the prior art, the present invention uses houttuynia cordata, honeysuckle and chymotrypsin in a specific ratio as main raw materials, and adopts a specific process to prepare a compound gel. Specifically, houttuynia cordata and honeysuckle are first soaked to allow the effective ingredients therein to be better extracted, then the effective ingredients in houttuynia cordata and honeysuckle are extracted by distillation, and then chymotrypsin and carbomer, moisturizer, propylene glycol, pH regulator and water are added to obtain a compound gel. The houttuynia cordata extract in the compound gel has a good inhibitory effect on Staphylococcus aureus, which is easy to cause animal mastitis, and the houttuynia cordata extract also has the effects of anti-virus, improving body immunity and promoting tissue regeneration. The honeysuckle extract contains ingredients such as chlorogenic acid and luteolin glycosides, which also has a strong inhibitory force on Staphylococcus aureus, and also has the effects of enhancing immunity and anti-inflammatory. Chymotrypsin can quickly decompose denatured proteins, and has the effects of anti-inflammatory and promoting tissue recovery. Carbomer is used to increase the viscosity of the compound gel to facilitate external application, and the moisturizer and propylene glycol help improve the skin feel and stability of the compound gel, which is conducive to external application. The compound gel prepared by the method of the present invention can be applied to the breast inflammation area of ​​the animal through the drug patch of the ultrasonic drug permeation instrument or directly, without the first-pass effect of the liver, reducing the damage of the gastrointestinal digestive juice to the drug, and the compound gel has a good effect on treating animal mastitis. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 The present invention is a schematic flow chart of a method for preparing a compound gel for treating animal mastitis according to an embodiment of the present invention. DETAILED DESCRIPTION

[0022] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the specific embodiments of the present invention are described in detail below in conjunction with the accompanying drawings. Although certain embodiments of the present invention are shown in the accompanying drawings, it should be understood that the present invention can be implemented in various forms and should not be interpreted as being limited to the embodiments described herein. On the contrary, these embodiments are provided to provide a more thorough and complete understanding of the present invention. It should be understood that the drawings and embodiments of the present invention are only for exemplary purposes and are not intended to limit the scope of protection of the present invention.

[0023] Unless otherwise defined, all technical and scientific terms used in this application have the same meanings as those commonly understood by those skilled in the art to which this application belongs; the terms used in the specification of this application are only for the purpose of describing specific implementation methods and are not intended to limit this application;

[0024] The term "including" and its variations used in this article are open inclusions, that is, "including but not limited to"; the term "based on" is "based at least in part on"; the term "one embodiment" means "at least one embodiment"; the term "another embodiment" means "at least one other embodiment"; the term "some embodiments" means "at least some embodiments"; the term "optionally" means "optional embodiments". The relevant definitions of other terms will be given in the following description. It should be noted that the concepts of "first" and "second" mentioned in the present invention are used to distinguish different objects, rather than to describe a specific order or a primary and secondary relationship. In addition, the terms "first" and "second" are used only for descriptive purposes and cannot be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Therefore, the features defined as "first" and "second" may explicitly or implicitly include one or more of the features. In the description of this application, unless otherwise specified, the meaning of "multiple" is two or more.

[0025] like Figure 1 As shown, a method for preparing a compound gel for treating animal mastitis provided by an embodiment of the present invention comprises:

[0026] Step S1, moistening Houttuynia cordata and honeysuckle with water, and then soaking them in water;

[0027] Step S2, steam distilling the soaked houttuynia and honeysuckle, collecting the distillate, and obtaining a primary distillate; the ratio of the total mass of the houttuynia and honeysuckle to the mass of the primary distillate is 1:(0.9-1.1); it should be noted that the "total mass of the houttuynia and honeysuckle" here refers to the total mass of the unsoaked houttuynia and unsoaked honeysuckle;

[0028] Step S3, redistillating the primary distillate, collecting the distillate to obtain a heavy distillate; the mass ratio of the heavy distillate to the primary distillate is 1:(4-6);

[0029] Step S4, adding chymotrypsin to the heavy distillate, stirring evenly, adding carbomer, mixing evenly, adding moisturizer and propylene glycol in sequence, stirring evenly, standing for a preset time, adding a pH regulator, adjusting the pH value to 6.8-7.2, adding water, stirring evenly, and obtaining a compound gel;

[0030] The raw materials used in the preparation of the compound gel are as follows: 2000-3000 parts of houttuynia cordata, 2000-3000 parts of honeysuckle, 2-5 parts of chymotrypsin, 20-40 parts of carbomer, 30-50 parts of moisturizer, and 20-30 parts of propylene glycol, by weight. It should be noted that the "houttuynia cordata" and "honeysuckle" here refer to houttuynia cordata without soaking treatment and honeysuckle without soaking treatment, respectively.

[0031] The embodiment of the present invention uses houttuynia cordata, honeysuckle and chymotrypsin in a specific ratio as the main raw materials, and adopts a specific process to prepare a compound gel. Specifically, houttuynia cordata and honeysuckle are first soaked so that the effective ingredients therein can be better extracted, and then the effective ingredients in houttuynia cordata and honeysuckle are extracted by distillation, and then chymotrypsin and carbomer, moisturizer, propylene glycol, pH regulator and water are added to obtain a compound gel. The houttuynia cordata extract in the compound gel has a good inhibitory effect on Staphylococcus aureus, which is easy to cause animal mastitis, and the houttuynia cordata extract also has the effects of anti-virus, improving body immunity and promoting tissue regeneration. The honeysuckle extract contains ingredients such as chlorogenic acid and luteolin glycosides, which also has a strong inhibitory force on Staphylococcus aureus, and also has the effects of enhancing immunity and anti-inflammatory. Chymotrypsin can quickly decompose denatured proteins, and has the effects of anti-inflammatory and promoting tissue recovery. Carbomer is used to increase the viscosity of the compound gel for external application, and the moisturizer and propylene glycol help improve the skin feel and stability of the compound gel, which is conducive to external application. The compound gel prepared by the method of the embodiment of the present invention can be applied to the breast inflammation area of ​​the animal through the drug patch of the ultrasonic drug permeation instrument or directly, without the first-pass effect of the liver, reducing the damage of the gastrointestinal digestive juice to the drug, and the compound gel has a good effect in treating animal mastitis.

[0032] In some embodiments of the present invention, in step S1, the soaking treatment time is 20-40 minutes.

[0033] In some embodiments of the present invention, in step S1, the ratio of the total mass of the houttuynia cordata and honeysuckle to the mass of the water used for the soaking treatment is 1:(5-6).

[0034] In some embodiments of the present invention, in step S4, the preset time is 3-5 hours.

[0035] In some embodiments of the present invention, in step S4, the pH adjuster is triethanolamine.

[0036] In some embodiments of the present invention, in step S4, the moisturizing agent is glycerol.

[0037] In some embodiments of the present invention, in the step S4, the amount of the compound gel is 9500-10500 parts by weight.

[0038] In some embodiments of the present invention, the water used in the preparation process of the composite gel is purified water, such as deionized water.

[0039] In some embodiments of the present invention, in step S2, the ratio of the total mass of the herb Houttuynia cordata and the flower honeysuckle to the mass of the primary distillate is 1:1.

[0040] The embodiment of the present invention further provides a compound gel for treating animal mastitis, which is prepared by the preparation method of the compound gel for treating animal mastitis as described above.

[0041] The present invention is further described below in conjunction with specific embodiments.

[0042] Example 1

[0043] A1. Wetting 2000 g of Houttuynia cordata and 2000 g of Honeysuckle with deionized water, and then soaking them in deionized water; wherein the soaking time is 30 min, and the ratio of the total mass of the Houttuynia cordata and Honeysuckle to the mass of the deionized water used for the soaking is 1:5.

[0044] A2. Steam distilling the soaked Houttuynia cordata and honeysuckle, collecting the distillate to obtain a primary distillate; the mass of the primary distillate is 4000 g.

[0045] A3. Re-distilling the primary distillate, collecting the distillate to obtain a heavy distillate; the mass ratio of the heavy distillate to the primary distillate is 1:5.

[0046] A4. Add 5 g of chymotrypsin to the heavy distillate, stir evenly, add 20 g of carbomer, mix evenly, add 30 g of glycerol and 20 g of propylene glycol in sequence, stir evenly, let stand for 4 hours, add pH regulator triethanolamine, adjust the pH value to 6.8-7.2, add deionized water, stir evenly, and obtain a compound gel; the weight of the compound gel is 10000 g.

[0047] Example 2

[0048] A1. Wetting 3000 g of Houttuynia cordata and 2500 g of Honeysuckle with deionized water, and then soaking them in deionized water; wherein the soaking time is 30 min, and the ratio of the total mass of the Houttuynia cordata and Honeysuckle to the mass of the deionized water used for the soaking is 1:5.

[0049] A2. Steam distilling the soaked Houttuynia cordata and honeysuckle, collecting the distillate to obtain a primary distillate; the mass of the primary distillate is 5500 g.

[0050] A3. Re-distilling the primary distillate, collecting the distillate to obtain a heavy distillate; the mass ratio of the heavy distillate to the primary distillate is 1:5.

[0051] A4. Add 3 g of chymotrypsin to the heavy distillate, stir evenly, add 30 g of carbomer, mix evenly, add 40 g of glycerol and 25 g of propylene glycol in sequence, stir evenly, let stand for 5 h, add pH regulator triethanolamine, adjust the pH value to 6.8-7.2, add deionized water, stir evenly, and obtain a compound gel; the weight of the compound gel is 1000 g.

[0052] Example 3

[0053] A1. Wetting 3000 g of Houttuynia cordata and 3000 g of Honeysuckle with deionized water, and then soaking them in deionized water; wherein the soaking time is 30 min, and the ratio of the total mass of the Houttuynia cordata and Honeysuckle to the mass of the deionized water used for the soaking is 1:5.

[0054] A2. Steam distilling the soaked Houttuynia cordata and honeysuckle, collecting the distillate to obtain a primary distillate; the mass of the primary distillate is 6000 g.

[0055] A3. Re-distilling the primary distillate, collecting the distillate to obtain a heavy distillate; the mass ratio of the heavy distillate to the primary distillate is 1:5.

[0056] A4. Add 2 g of chymotrypsin to the heavy distillate, stir evenly, add 40 g of carbomer, mix evenly, add 50 g of glycerol and 30 g of propylene glycol in sequence, stir evenly, let stand for 5 h, add pH regulator triethanolamine, adjust the pH value to 6.8-7.2, add deionized water, stir evenly, and obtain a compound gel; the weight of the compound gel is 10000 g.

[0057] Comparative Example 1

[0058] The difference from Example 1 is that in step A4, chymotrypsin is not added.

[0059] Comparative Example 2

[0060] A1. Wetting 2000 g of honeysuckle with deionized water, and then soaking it in deionized water; wherein the soaking time is 30 min, and the ratio of the mass of the honeysuckle to the mass of the deionized water used for the soaking is 1:5.

[0061] A2. Steam distilling the soaked honeysuckle, collecting the distillate, and obtaining a primary distillate; the mass of the primary distillate is 4000 g.

[0062] A3. Re-distilling the primary distillate, collecting the distillate to obtain a heavy distillate; the mass ratio of the heavy distillate to the primary distillate is 1:5.

[0063] A4. Add 5 g of chymotrypsin to the heavy distillate, stir evenly, add 20 g of carbomer, mix evenly, add 30 g of glycerol and 20 g of propylene glycol in sequence, stir evenly, let stand for 4 hours, add pH regulator triethanolamine, adjust the pH value to 6.8-7.2, add deionized water, stir evenly, and obtain a compound gel; the weight of the compound gel is 10000 g.

[0064] Comparative Example 3

[0065] A1. Wetting 2000 g of Houttuynia cordata with deionized water, and then soaking it in deionized water; wherein the soaking time is 30 min, and the ratio of the mass of Houttuynia cordata to the mass of deionized water used for soaking is 1:5.

[0066] A2. Steam distilling the soaked Houttuynia cordata, collecting the distillate, and obtaining a primary distillate; the mass of the honeysuckle is 4000 g.

[0067] A3. Re-distilling the primary distillate, collecting the distillate to obtain a heavy distillate; the mass ratio of the heavy distillate to the primary distillate is 1:5.

[0068] A4. Add 5 g of chymotrypsin to the heavy distillate, stir evenly, add 20 g of carbomer, mix evenly, add 30 g of glycerol and 20 g of propylene glycol in sequence, stir evenly, let stand for 4 hours, add pH regulator triethanolamine, adjust the pH value to 6.8-7.2, add deionized water, stir evenly, and obtain a compound gel; the weight of the compound gel is 10000 g.

[0069] Comparative Example 4

[0070] A1. Wet 2000g of Houttuynia cordata and 2000g of Honeysuckle with deionized water, and boil them in 20kg of deionized water for 2 times, each time for 1.5 hours. Combine the decoctions, filter and concentrate the filtrate to a clear paste with a relative density of 1.14 (80°C).

[0071] A2. Add ethanol to the clear paste and stir evenly to obtain a mixed solution (the mass fraction of ethanol in the mixed solution is 75%); let the mixed solution stand for 24 hours, filter the supernatant, add an appropriate amount of ethanol to the residue, stir evenly, let stand for 12 hours, and filter; combine the ethanol solutions, recover the ethanol until there is no alcohol taste, add 5g of chymotrypsin, stir evenly, add 20g of carbomer, mix evenly, add 30g of glycerol and 20g of propylene glycol in sequence, stir evenly, let stand for 4 hours, add pH adjuster triethanolamine, adjust the pH value to 6.8-7.2, add deionized water, stir evenly, and obtain a compound gel; the weight of the compound gel is 10000g.

[0072] Comparative Example 5

[0073] A1. Wetting 2000 g of Houttuynia cordata and 2000 g of Honeysuckle with deionized water, and then soaking them in deionized water; wherein the soaking time is 30 min, and the ratio of the total mass of the Houttuynia cordata and Honeysuckle to the mass of the deionized water used for the soaking is 1:5.

[0074] A2. Steam distilling the soaked Houttuynia cordata and honeysuckle, collecting the distillate to obtain a primary distillate; the mass of the primary distillate is 4000 g.

[0075] A3. Re-distilling the primary distillate, collecting the distillate to obtain a heavy distillate; the mass ratio of the heavy distillate to the primary distillate is 1:5.

[0076] A4, 5g chymotrypsin was added to the heavy distillate, stirred evenly, 5g carboxyvinyl 941 was added, mixed evenly, 50g glycerol and 16g polyethylene glycol 4000 were added in sequence, stirred evenly, left to stand for 4h, pH adjusting agent triethanolamine was added, pH was adjusted to 6.8-7.2, deionized water was added, stirred evenly, and a compound gel was obtained; the weight of the compound gel was 1000g.

[0077] Experimental example

[0078] 1. In vitro antibacterial test

[0079] Preparation of test bacterial suspension: Staphylococcus aureus was streaked into mannitol sodium chloride agar medium and cultured at 37°C for 24 h; a single colony with a smooth surface and good growth was selected and inoculated into tryptone soy broth for pure culture, and then cultured in a constant temperature shaker (180 r / min) at 37°C for 24 h; after determining the logarithmic growth phase of Staphylococcus aureus by plate counting method, the number of viable bacteria in the bacterial solution in the logarithmic growth phase was determined, and the counted bacterial solution was adjusted to a bacterial solution concentration of 3.0×10 8 CFU / mL of Staphylococcus aureus suspension for later use.

[0080] Experimental Group 1

[0081] 50 μL of the test bacterial suspension was evenly coated on the mannitol sodium chloride agar medium, and a sterile puncher with a diameter of 4 mm was used to punch holes on each mannitol sodium chloride agar medium, and 50 μL of the compound gel was added to each hole. The mannitol sodium chloride agar medium after the above treatment was placed in an electric constant temperature incubator, and after culturing at 37°C for 24 hours, the size of the inhibition zone was measured using a vernier caliper; wherein the compound gel used was the compound gel prepared in Example 1.

[0082] Experimental Group 2

[0083] The difference from Experimental Group 1 is that the compound gel used is the compound gel prepared in Example 2.

[0084] Experimental Group 3

[0085] The difference from Experimental Group 1 is that the compound gel used is the compound gel prepared in Example 3.

[0086] Control group 1

[0087] The difference from Experimental Group 1 is that the compound gel used is the compound gel prepared in Comparative Example 1.

[0088] Control group 2

[0089] The difference from Experimental Group 1 is that the compound gel used is the compound gel prepared in Comparative Example 2.

[0090] Control group 3

[0091] The difference from Experimental Group 1 is that the compound gel used is the compound gel prepared in Comparative Example 3.

[0092] Control group 4

[0093] The difference from Experimental Group 1 is that the compound gel used is the compound gel prepared in Comparative Example 4.

[0094] Control group 5

[0095] The difference from Experimental Group 1 is that the compound gel used is the compound gel prepared in Comparative Example 5.

[0096] The test results of the experimental groups 1-3 and the control groups 1-5 are shown in Table 1. It can be seen from Table 1 that the compound gels used in the experimental groups 1-3 and the control groups 1-5 have different degrees of antibacterial effects on Staphylococcus aureus, among which Staphylococcus aureus is extremely sensitive to the compound gel in Example 1, highly sensitive to the compound gel in Examples 2-3, slightly sensitive to the compound gel in Comparative Example 1, and moderately sensitive to the compound gel in Comparative Examples 2-3, indicating that the compound gel in Examples 1-3 has a better antibacterial effect on Staphylococcus aureus.

[0097] Table 1

[0098] Group Diameter of inhibition zone (mm) Antibacterial level Experimental Group 1 22.06±0.15 ++++ Experimental Group 2 17.15±0.32 +++ Experimental Group 3 18.23±0.18 +++ Control group 1 8.85±0.17 + Control group 2 11.30±0.21 ++ Control group 3 10.68±0.12 ++ Control group 4 14.36±0.11 ++ Control group 5 15.09±0.08 ++

[0099] It should be noted that the inhibition zone determination criteria are: inhibition zone diameter > 20 mm, extremely sensitive (++++); inhibition zone diameter 15-20 mm, highly sensitive (+++); inhibition zone diameter 10-15 mm, moderately sensitive (++); inhibition zone diameter < 10 mm, low sensitivity (+); no inhibition zone, insensitive (-).

[0100] 2. In vitro anti-inflammatory test

[0101] Experimental Group 1

[0102] Take 5 female Kunming mice, and apply physiological saline, indomethacin ointment, and compound gel (0.5mL) inside and outside the left auricle of each mouse, respectively, once every 15 minutes, for 3 consecutive times. 30 minutes after the third application, 0.05mL of xylene was applied to the left ear of each mouse to cause inflammation, and the right ear was used as a control. 30 minutes after inflammation, cut off both ears, and use a puncher with a diameter of 8mm to punch round ear pieces at the same part of the left and right ears, respectively, weigh them, and calculate the swelling degree and swelling inhibition rate; wherein, the compound gel used is the compound gel prepared in Example 1.

[0103] Experimental Group 2

[0104] The difference from Experimental Group 1 is that the compound gel used is the compound gel prepared in Example 2.

[0105] Experimental Group 3

[0106] The difference from Experimental Group 1 is that the compound gel used is the compound gel prepared in Example 3.

[0107] Control group 1

[0108] The difference from Experimental Group 1 is that the compound gel used is the compound gel prepared in Comparative Example 1.

[0109] Control group 2

[0110] The difference from Experimental Group 1 is that the compound gel used is the compound gel prepared in Comparative Example 2.

[0111] Control group 3

[0112] The difference from Experimental Group 1 is that the compound gel used is the compound gel prepared in Comparative Example 3.

[0113] Control group 4

[0114] The difference from Experimental Group 1 is that the compound gel used is the compound gel prepared in Comparative Example 4.

[0115] Control group 5

[0116] The difference from Experimental Group 1 is that the compound gel used is the compound gel prepared in Comparative Example 5.

[0117] Blank control group

[0118] The difference from Experimental Group 1 is that the compound gel used was replaced by water.

[0119] The test results of the experimental groups 1-3, the control groups 1-5 and the blank control group are shown in Table 2. It can be seen from Table 2 that the experimental groups 1-3 have a significant inhibitory effect on the ear swelling of mice caused by xylene, which is statistically significant compared with the blank control group (P<0.01). The control groups 1-5 all have a certain inhibitory effect, and the difference is statistically significant compared with the blank control group (P<0.05), indicating that the anti-inflammatory effect of the compound gel in Example 1-3 is better.

[0120] Table 2

[0121] Group Swelling degree / mg Swelling inhibition rate / % Experimental Group 1 1.78±0.36 55.35 Experimental Group 2 1.83±0.34 54.09 Experimental Group 3 1.90±0.22 52.20 Control group 1 2.63±0.17 33.96 Control group 2 2.38±0.45 40.25 Control group 3 2.48±0.48 37.74 Control group 4 2.30±0.24 42.14 Control group 5 2.35±0.50 40.88 Blank control group 3.98±0.42 -

[0122] It should be noted that swelling degree = weight of left ear piece - weight of right ear piece, swelling inhibition rate = (swelling degree of blank control group - swelling degree of drug administration group) / swelling degree of blank control group × 100%. Among them, "-" in the table means that relevant data were not detected.

[0123] 3. Experiment on the efficacy of treating mastitis in dairy cows

[0124] Experimental Group 1

[0125] Twenty 5-year-old cows with clinical bovine mastitis and 3-4 litters were selected from a dairy farm in Anda City, Heilongjiang Province. Ultrasonic transdermal administration was performed on the base of the inflamed nipple area of ​​each cow once a day for 5 consecutive days, and the number of cured cows was counted; wherein the drug used for ultrasonic transdermal administration was the compound gel prepared in Example 1.

[0126] Experimental Group 2

[0127] The difference from Experimental Group 1 is that the compound gel used is the compound gel prepared in Example 2.

[0128] Experimental Group 3

[0129] The difference from Experimental Group 1 is that the compound gel used is the compound gel prepared in Example 3.

[0130] Control group 1

[0131] The difference from Experimental Group 1 is that the compound gel used is the compound gel prepared in Comparative Example 1.

[0132] Control group 2

[0133] The difference from Experimental Group 1 is that the compound gel used is the compound gel prepared in Comparative Example 2.

[0134] Control group 3

[0135] The difference from Experimental Group 1 is that the compound gel used is the compound gel prepared in Comparative Example 3.

[0136] Control group 4

[0137] The difference from Experimental Group 1 is that the compound gel used is the compound gel prepared in Comparative Example 4.

[0138] Control group 5

[0139] The difference from Experimental Group 1 is that the compound gel used is the compound gel prepared in Comparative Example 5.

[0140] The statistical results of the experimental groups 1-3 and the control groups 1-5 are shown in Table 3. It can be seen from Table 3 that the compound gel prepared in Examples 1-3 has a better therapeutic effect on bovine mastitis.

[0141] Table 3

[0142]

[0143]

[0144] Although the present invention is disclosed as above, the protection scope of the present invention is not limited thereto. Those skilled in the art may make various changes and modifications without departing from the spirit and scope of the present invention, and these changes and modifications will fall within the protection scope of the present invention.

Claims

1. A method for preparing a compound gel for treating animal mastitis, characterized in that: include: Step S1, moistening Houttuynia cordata and honeysuckle with water, and then soaking them in water; Step S2, steam distilling the soaked Houttuynia cordata and honeysuckle, collecting the distillate to obtain a primary distillate; the ratio of the total mass of the Houttuynia cordata and the honeysuckle to the mass of the primary distillate is 1:(0.9-1.1); Step S3, redistillating the primary distillate, collecting the distillate to obtain a heavy distillate; the mass ratio of the heavy distillate to the primary distillate is 1:(4-6); Step S4, adding chymotrypsin to the heavy distillate, stirring evenly, adding carbomer, mixing evenly, adding moisturizer and propylene glycol in sequence, stirring evenly, standing for a preset time, adding a pH regulator, adjusting the pH value to 6.8-7.2, adding water, stirring evenly, and obtaining a compound gel; Among them, the amounts of raw materials used in the preparation process of the compound gel are as follows, by weight: 2000-3000 parts of Houttuynia cordata, 2000-3000 parts of honeysuckle, 2-5 parts of chymotrypsin, 20-40 parts of carbomer, 30-50 parts of moisturizer, and 20-30 parts of propylene glycol.

2. The method for preparing the compound gel for treating animal mastitis according to claim 1, characterized in that: In the step S1, the soaking treatment time is 20-40 minutes.

3. The preparation method of the compound gel for treating animal mastitis according to claim 1, characterized in that: In the step S1, the ratio of the total mass of the houttuynia cordata and honeysuckle to the mass of the water used for the soaking treatment is 1:(5-6).

4. The method for preparing the compound gel for treating animal mastitis according to claim 1, characterized in that: In step S4, the preset time is 3-5 hours.

5. The method for preparing the compound gel for treating animal mastitis according to claim 1, characterized in that: In the step S4, the pH adjuster is triethanolamine.

6. The method for preparing the compound gel for treating animal mastitis according to claim 1, characterized in that: In the step S4, the moisturizing agent is glycerin.

7. The method for preparing the compound gel for treating animal mastitis according to claim 1, characterized in that: In terms of weight, in step S4, the amount of the compound gel is 9500-10500 parts.

8. The method for preparing the compound gel for treating animal mastitis according to claim 1, characterized in that: The water used in the preparation process of the compound gel is purified water.

9. The method for preparing the compound gel for treating animal mastitis according to claim 1, characterized in that: In the step S2, the ratio of the total mass of the herb Houttuynia cordata and the honeysuckle to the mass of the primary distillate is 1:

1.

10. A compound gel for treating animal mastitis, characterized in that: The compound gel for treating animal mastitis is prepared by the preparation method of any one of claims 1 to 9.

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