Oil tea bran organic fertilizer as well as preparation method and application thereof

Abstract

The invention provides an oil tea bran organic fertilizer as well as a preparation method and application thereof, and belongs to the technical field of agricultural fertilizers. The method comprises the following steps: mixing livestock manure, camellia oleifera bran and plant-derived organic waste according to a mass ratio of (50-60): (20-25): (15-20), and fermenting twice to obtain the organic fertilizer. The camellia oleifera bran organic fertilizer is applied before plant planting, root pathogenic nematode detection is carried out three months after loofah field planting, no root knot nematode disease is found on plants to which the camellia oleifera bran organic fertilizer is applied, and the pathogenic nematode morbidity of the plants to which the camellia oleifera bran organic fertilizer is not applied is 87.26%; the organic fertilizer is applied before field planting of cucumbers until seedlings are pulled out, and the effect is better than that of a chemical agent fosthiazate. The organic fertilizer containing the camellia oleifera bran provides a new thought for prevention and treatment of root-knot nematodes of cowpeas, towel gourds and cucumbers. The organic fertilizer is low in raw material cost, easy to obtain, simple in preparation method and suitable for large-scale popularization and application.

Description

Technical Field

[0001] The invention relates to the technical field of agricultural fertilizers, and in particular to an oil-tea camellia bran organic fertilizer and a preparation method and application thereof. Background Art

[0002] Root-knot nematodes (Meloidogyne spp.) mainly parasitize on plant roots, interfere with plant nutrition and water absorption, and seriously restrict vegetable production. Due to its wide host range, short life history, and high reproduction rate, the economic losses caused by soil-borne diseases account for more than 90% of crop losses, and it is considered to be one of the most harmful plant pathogens in the world. According to incomplete statistics, the average annual agricultural production reduction caused by root-knot nematodes around the world is 24.5%, causing economic losses of more than 100 billion yuan. Since the currently available vegetable germplasm resources resistant to root-knot nematodes are very scarce, and many crops such as cucurbitaceae and leguminous crops have not yet been found to have resistant resources. Therefore, chemical nematicides are still the mainstream of the market, mainly carbamates and organophosphates, such as aldicarb, carbofuran, thiazothiazide, rufuda and lime nitrogen, which are highly toxic, highly residual, easy to develop drug resistance, extremely unsafe for humans and animals, difficult to degrade, seriously damage soil structure, and have no obvious effect on nematode eggs, which are criticized.

[0003] Camellia olefera Abel. is a woody edible oil tree species unique to southern my country and one of the most important oil plants in my country. As of 2018, my country's camellia planting area exceeded 70 million mu, with an annual output of 2.5 million tons of tea oil. The byproduct of camellia oil extraction - camellia bran cake (also known as tea seed cake, tea dregs) has an annual output of 12 to 14 million tons. Camellia olefera bran cake contains 15 to 18% tea saponin, oil content <2%, protein 12 to 16%, starch and sugar 30 to 50%, fiber 10 to 12%. The organic acids, nitrogen-containing compounds and thioglucosidase derivatives produced by decomposition in the soil will affect soil pH, redox capacity and increase soil microbial activity. However, there is a lack of research on the treatment of vegetable continuous cropping soil containing root-knot nematodes with camellia bran, and it cannot be effectively promoted on a large scale. The purpose of the present invention is to provide an organic fertilizer for preventing and controlling root-knot nematodes and a preparation method thereof, and to mix and ferment camellia bran cakes with organic waste materials such as animal and plant residues, livestock and poultry excrement, compost, green manure and high-protein waste, so as to provide technical support for the green prevention and control application of camellia bran in root-knot nematodes and fill the gap in the relevant field. Summary of the invention

[0004] The invention aims to provide an organic fertilizer of camellia bran and a preparation method and application thereof, so as to solve the problems of environmental pollution and pesticide residue caused by chemical agents such as aldicarb, carbofuran, thiamethoxam, rufodin and lime nitrogen for controlling root-knot nematodes.

[0005] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0006] The invention provides a method for preparing oil-tea camellia bran organic fertilizer, comprising the following steps:

[0007] (1) mixing plant-derived organic waste, tea bran and livestock manure, adjusting the moisture content of the mixture to between 50% and 65%, adding a microbial agent, and performing high-temperature aerobic fermentation after compaction to obtain a fermented material after 20 to 25 days;

[0008] (2) Piling the fermentation material in step (1) into a pile for anaerobic low-temperature fermentation, and completing the fermentation in 30 to 35 days;

[0009] (3) drying the fermented material until the moisture content is ≤10%, and then granulating the material to obtain the organic fertilizer.

[0010] Preferably, the mass ratio of the poultry and livestock manure, tea bran and plant-based organic waste is 50-60:20-25:15-20.

[0011] Preferably, the plant-derived organic waste includes one or more of wheat, rice, corn, sorghum and cotton straw, rice husks, corn cobs, bagasse, beet pulp and fruit shells.

[0012] Preferably, the carbon-nitrogen ratio of the mixed material is 20 to 35:1, and the pH is 5.5 to 8.5.

[0013] Preferably, the amount of the microbial agent added is 1 to 2‰ of the mass of the mixed material.

[0014] Preferably, during the high temperature aerobic fermentation stage, when the temperature of the pile is lower than 55°C, oxygen is supplied by turning the pile, and when the temperature of the pile is higher than 65°C, the temperature is lowered by turning the pile.

[0015] The invention also provides tea bran organic fertilizer prepared by the preparation method.

[0016] The present invention also provides the application of the oil-tea camellia bran organic fertilizer in at least one of the following:

[0017] (1) Application in the prevention and treatment of root-knot nematodes in cucurbitaceae and leguminous plants;

[0018] (2) Application of the organic fertilizer in promoting plant growth and / or increasing yield.

[0019] The present invention also provides a method for preventing and controlling root-knot nematodes of crops, which comprises spreading the oil-tea bran organic fertilizer according to claim 7 before planting the crops.

[0020] By adopting the above technical scheme, the present invention has the following beneficial effects: the oil tea bran organic fertilizer of the present invention is spread before the plants are planted, and the root pathogenic nematodes are detected 3 months after the loofah is planted. The plants to which the oil tea bran organic fertilizer is applied are not found to have root-knot nematode diseases, and the incidence of pathogenic nematodes in the plants not applied with the oil tea bran organic fertilizer is 87.26%; the organic fertilizer is applied before the cucumber is planted, and no disease is found until the seedlings are pulled out, and the effect is better than the chemical agent thiazolyl. The organic fertilizer containing oil tea bran of the present invention provides a new idea for the prevention and control of root-knot nematodes of cowpea, loofah and cucumber. The raw material cost of the organic fertilizer of the present invention is low, easy to obtain, and the preparation method is simple, which is suitable for large-scale promotion and application. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 This is a comparison chart of the growth of cowpea plants on August 14, with the experimental group on the left and the control group on the right.

[0022] Figure 2 This is a comparison chart of plant growth on October 1st, with the experimental group on the left and the control group on the right.

[0023] Figure 3 The damage caused by root-knot nematodes on the roots of cowpea.

[0024] Figure 4 The incidence of root pathogenic nematodes in loofahs of different treatment groups 3 months after planting.

[0025] Figure 5 This is a comparison of cucumbers in different treatment groups 20 days after planting.

[0026] Figure 6 This is the incidence of root pathogenic nematodes in cucumbers of different treatment groups 2 months after planting.

[0027] Figure 7 Root-knot nematodes observed under a microscope. DETAILED DESCRIPTION

[0028] The technical solutions provided by the present invention are described in detail below in conjunction with the embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0029] The camellia bran in the embodiment of the present invention is a byproduct of camellia seed oil extraction - camellia bran, which comes from Yichun Qiansheng Agricultural Development Co., Ltd.

[0030] Example 1

[0031] Collect chicken manure, separate the dry and wet parts, and keep the solid manure for later use. Put the above manure into a blender and stir, while adding tea bran, wheat straw and EM inoculant. The mass ratio of chicken manure, tea bran and wheat straw is 50:20:15, and the moisture content of the mixture is adjusted to 50% and the pH is 6.0. The amount of EM inoculant added is 1‰ of the total mass of the mixture.

[0032] The prepared compost material is compacted and covered before composting. The high-temperature aerobic fermentation lasts for 20 days. The high-temperature process of 55℃~65℃ lasts for no less than 15 days. When the temperature of the pile is lower than 55℃, oxygen is supplied by turning the pile. When the temperature of the pile is higher than 65℃, the temperature is lowered by turning the pile. After one fermentation, the moisture content of the material is reduced, and harmful substances such as pest eggs and grass seeds are inactivated, meeting the requirements of harmlessness; at the same time, the macromolecular organic matter is decomposed and matured to form small molecular substances; the permeability of the material is enhanced, the color is deepened, and the humification is basically completed, and the fermented material is obtained.

[0033] The fermented materials are transferred to a large pile indoors for anaerobic low-temperature fermentation for 30 days, during which the pile is turned 2 to 3 times. Through secondary fermentation, the materials are thoroughly decomposed, the moisture content is further reduced, the looseness of the materials is enhanced, the color is deepened, the odor is completely eliminated, the low-temperature beneficial microorganisms multiply and form hyphae, and small molecular organic substances are synthesized.

[0034] The material after the secondary fermentation is dried in the sun until the moisture content is ≤10%, and the material is stirred again to make it uniform, and the material is extruded and granulated to form cylindrical particles.

[0035] Example 2

[0036] Collect pig manure, separate the dry and wet parts, and keep the solid manure for later use. Put the above manure into a mixer and stir it. Add tea bran, rice husk and EM inoculant while stirring. The mass ratio of pig manure, tea bran and rice husk is 55:23:18. Adjust the moisture content of the mixture to 60% and pH 7.0. The amount of EM inoculant added is 1.5‰ of the total mass of the mixture.

[0037] The prepared compost material is compacted and covered before composting. The high-temperature aerobic fermentation lasts for 22 days. The high-temperature process of 55℃~65℃ lasts for no less than 15 days. When the temperature of the pile is lower than 55℃, the pile is turned to supply oxygen. When the temperature of the pile is higher than 65℃, the pile is turned to cool down. After one fermentation, the moisture content of the material is reduced, and harmful substances such as pest eggs and grass seeds are inactivated, meeting the requirements of harmlessness; at the same time, the macromolecular organic matter is decomposed and matured to form small molecular substances; the permeability of the material is enhanced, the color is deepened, and the humification is basically completed, and the fermented material is obtained.

[0038] The fermented materials are transferred to a large pile indoors for anaerobic low-temperature fermentation for 32 days, during which the pile is turned 2 to 3 times. Through secondary fermentation, the materials are thoroughly decomposed, the moisture content is further reduced, the looseness of the materials is enhanced, the color is deepened, the odor disappears completely, the low-temperature beneficial microorganisms multiply and form hyphae, and small molecular organic substances are synthesized.

[0039] The material after the secondary fermentation is dried in the sun until the moisture content is ≤10%, and the material is stirred again to make it uniform, and the material is extruded and granulated to form cylindrical particles.

[0040] Example 3

[0041] Collect cow dung, separate the dry and wet parts, and keep the solid dung for later use. Put the above dung into a mixer and stir, while adding tea bran, corn stalks and EM inoculant. The mass ratio of cow dung, tea bran and corn stalks is 60:25:20, and the moisture content of the mixture is adjusted to 65% and the pH is 8.0. The amount of EM inoculant added is 2‰ of the total mass of the mixture.

[0042] The prepared compost material is compacted and covered before composting. The high-temperature aerobic fermentation lasts for 25 days. The high temperature process of 55℃~65℃ lasts for no less than 15 days. When the temperature of the pile is lower than 55℃, the pile is turned to supply oxygen. When the temperature of the pile is higher than 65℃, the pile is turned to cool down. After one fermentation, the moisture content of the material is reduced, and harmful substances such as pest eggs and grass seeds are inactivated, meeting the requirements of harmlessness; at the same time, the macromolecular organic matter is decomposed and matured to form small molecular substances; the permeability of the material is enhanced, the color is deepened, and the humification is basically completed, and the fermented material is obtained.

[0043] The fermented materials are transferred to a large pile indoors for anaerobic low-temperature fermentation for 35 days, during which the pile is turned 2 to 3 times. Through secondary fermentation, the materials are thoroughly decomposed, the moisture content is further reduced, the looseness of the materials is enhanced, the color is deepened, the odor is completely eliminated, the low-temperature beneficial microorganisms multiply and form hyphae, and small molecular organic substances are synthesized.

[0044] The material after the secondary fermentation is dried in the sun until the moisture content is ≤10%, and the material is stirred again to make it uniform, and the material is extruded and granulated to form cylindrical particles.

[0045] Comparative Example 1

[0046] The difference from Example 1 is that no camellia oil bran is added.

[0047] The test variety for this experiment is the "Tianmei 20" bitter melon produced by Fujian Tianmei Seed Technology Co., Ltd., which is a widely cultivated variety in the Jiangxi and Fujian regions.

[0048] The organic fertilizers prepared in Examples 1 to 3 and Comparative Example 1 were tested for indicators such as the mass fraction of organic matter, total nutrients, moisture, pH, seed germination index, mass fraction of mechanical impurities, total arsenic, total mercury, total lead, total cadmium, total chromium, fecal coliform count, ascaris egg mortality rate, mass fraction of chloride ions, and weed seed activity, and all met the requirements of the standard "NY / T 525 Organic Fertilizer".

[0049] Experimental Example 1

[0050] The experiment was conducted in the standardized vegetable greenhouse of Wanxing Experimental Base of the National Vegetable Quality Standard Center (Ganzhou). On August 10, 2024, the vegetables were transplanted and planted in the greenhouse, planted in single rows, and cultivated in ridges. The height of the ridge was 25-30cm, the width of the ridge was 75-80cm, the spacing between plants was 150cm, and the rows were single. The experiment was divided into 5 groups, namely the control group (rice husk and chicken manure organic fertilizer), T1 (rice husk and chicken manure organic fertilizer containing 10% camellia bran), T2 (rice husk and chicken manure organic fertilizer containing 20% ​​camellia bran), T3 (rice husk and chicken manure organic fertilizer containing 30% camellia bran), and T4 (rice husk and chicken manure organic fertilizer containing 40% camellia bran). The area of ​​the plot is 208m 2 (26.0m×8.0m), 1.5m wide protection rows are set around the plot. The test group evenly spreads the organic fertilizer in Example 1 during land preparation and ridge making, and performs rotary tillage. Each group is set up with 3 repetitions, a total of 8 plots, and random block groups are arranged. September 15 to December 15, 2024 is the product harvest period. On November 29, 2024, relevant experts were organized to carry out field yield measurement. The yield measurement was carried out using the 5-point positioning method. Three plants were sampled continuously at each point to calculate the yield per unit area. After pulling the seedlings, the number of pathogenic nematodes in the plant roots was investigated.

[0051] The yield measurement results showed that the control group had a yield of 2748.64kg / 667m 2 The equivalent output of T1 is 3253.12kg / 667m 2 The equivalent output of T2 is 3687.51kg / 667m 2 The equivalent output of T3 is 3547.25kg / 667m 2 The equivalent output of T4 is 3521.74kg / 667m 2 . After pulling up the seedlings, the five-point sampling method was used to count the number of pathogenic nematodes in the soil. The results showed that the control group contained an average of 5.50, T1 contained 0.54, T2 contained 0.25, T3 contained 0.25, and T4 contained 0.25. From the above examples, it can be seen that a concentration of camellia bran greater than 20% has a better control effect on bitter melon root-knot nematodes. However, the organic acids, nitrogen-containing compounds and thioglucosidase derivative compounds produced by the decomposition of camellia bran in the soil have a partial inhibitory effect on plant growth when the concentration is too high. Therefore, a concentration of 20% to 30% of camellia bran is more appropriate.

[0052] Experimental Example 2

[0053] The test variety in this experiment is the "Zhijiang 616" cowpea produced by Nongwang Seed Industry, which is a widely cultivated variety in the area south of the Yangtze River.

[0054] The experiment was conducted in the standardized vegetable greenhouse of Wanxing Experimental Base of the National Vegetable Quality Standard Center (Ganzhou) Branch on July 30, 2024. Cowpea was sown in the greenhouse on July 30, 2024, planted in single rows, cultivated in ridges, with a ridge height of 40 cm and a ridge width of 80 cm. There were 2 to 3 seeds per hole and a seed rate of 1.5 kg / mu. The experiment was divided into two groups, the experimental group and the control group. The plot area of ​​the experimental group and the control group was 208m 2 (26.0m×8.0m), and 1.5m wide protective rows were set around the plot. During the land preparation and ridge making, the test group evenly spread the organic fertilizer in Example 1 and performed rotary tillage. The spreading rate was 1 ton / 667m 2 The control group spread the organic fertilizer in Example 1, and the spreading amount was the same as that of the experimental group. Each group was set up with 4 repetitions, a total of 8 plots, and the random block group arrangement. August 30, 2024 to October 15, 2024 is the product harvest period.

[0055] The seedling period, flowering period, harvest period, final harvest period, harvest period and the whole growth period of the experimental group and the control group were investigated. The seedling period was based on the emergence of more than 85% of the cowpea seedlings, and the flowering period was based on the flowering rate of more than 20%. The cowpea was harvested for the first time on August 30 and the last time on October 15. The calculation dates of the harvest period and the final harvest period were calculated when the harvest amount was ≥4kg / mu. Harvesting was carried out every morning. Each time the harvest was carried out, each plot was weighed and calculated separately, and the per mu yield was converted. The results are shown in Table 1.

[0056] Table 1 Effects of different treatments on the growth period of cowpea

[0057] month Sowing period Seedling stage initial flowering period First collection period Final Collection Period Harvest period / d Whole reproductive period / d Control group 7 / 30 8 / 03 8 / 21 8 / 31 10 / 01 31 58 Experimental Group 7 / 30 8 / 03 8 / 22 8 / 30 10 / 15 46 73

[0058] It can be seen from Table 1 that adding tea oil bran to organic fertilizer can prolong the harvest period of cowpea, thereby increasing the yield of cowpea.

[0059] The growth of the two groups of cowpea plants was observed on August 14 and October 1, respectively. Figure 1 and Figure 2 As shown. The picture shows that the cowpea in comparative example 1 showed leaf yellowing 15 days after planting, and premature aging of the plant 60 days after planting. At the peak of harvest (August 20), 5 holes were randomly selected from each plot to measure the SPAD value of the plant. On October 16, the cowpea seedlings were pulled, and the number of root knots, yield and commercial pod rate were counted. The data statistical method used Microsoft office Excel 2020 and SPSS22.0 statistical software to perform mathematical statistical analysis on the yield data. The results are shown in Table 2.

[0060] Table 2 Effects of different treatments on agronomic traits of cowpea

[0061] deal with SPAD value Number of root knots <![CDATA[Yield kg / 667m 2 > Product pod rate (%) Control group 29.64±2.14 24.53±0.89 258.76±3.41 42.34±1.35％ Experimental Group 45.68±0.32 0.72±0.37 1187.33±2.84 96.76±0.97％

[0062] The SPAD value is an indicator of the relative content of chlorophyll in plant leaves, which reflects the greenness and health of the plant. The SPAD value is proportional to the chlorophyll content of the plant. Generally, the higher the chlorophyll content, the higher the SPAD value, indicating that the plant has a stronger photosynthesis ability and is in better health.

[0063] The results showed that the leaves of the control group began to turn yellow after August 14, and the plants grew weakly. The roots had obvious knots, and due to insufficient nutrient supply, they showed problems such as premature aging and poor cowpea quality. It can be seen that adding tea bran to organic fertilizer can significantly reduce the number of root knots in cowpea ( Figure 3 ), the growth yield and commercial pod rate of cowpea have been greatly improved.

[0064] Experimental Example 3

[0065] The test variety in this experiment is the "Youbi No. 1" loofah produced by Dingpai Seed Industry, which is a widely cultivated variety in the area south of the Yangtze River.

[0066] The experiment was carried out on January 16, 2023 in the standardized vegetable greenhouse of Wanxing Experimental Base of the National Vegetable Quality Standard Center (Ganzhou), with a plant spacing of 35 cm, a row spacing of 40 cm, and two rows in one bed. The experiment was divided into two groups, the experimental group and the control group. The plot area of ​​the experimental group and the control group was 208m 2 (26.0m×8.0m), 1.5m wide protective rows were set around the plot, and the organic fertilizer in Example 3 was evenly spread during the land preparation and ridge making in the experimental group. The spreading rate was 1 ton / 667m 2 , the control group applied the organic fertilizer in comparative example 1, and the application amount was the same as that of the experimental group. Each group was set up with 4 replications, a total of 8 plots, and random block groups. The product harvest period is from April 1, 2024 to August 30, 2024. On August 29, 2024, relevant experts were organized to carry out field yield measurement. The yield measurement was carried out using the 5-point positioning method. Three plants were sampled continuously at each point to calculate the number of melons per plant and the weight of a single melon. The yield per unit area was converted according to the planting density, and the number of pathogenic nematodes in the plant root system was investigated.

[0067] The test results showed that the growth of the control group plants was weak. The yield measurement results showed that the average number of melons per plant in the experimental group was 11.63, and the average weight of a melon was 0.41kg, while the average number of melons per plant in the control group was 8.34, and the average weight of a melon was 0.35kg, which were 39.45% and 17.14% lower respectively. The equivalent yield of the experimental group was 5723.16kg / 667m 2 The control group's equivalent yield was 3523.92kg / 667m 2 , the yield decreased by 38.43%. After pulling out the seedlings, the number of pathogenic nematodes on the plants was counted. The incidence of pathogenic nematodes in the control group was 87.26% ( Figure 4). It can be seen from the above examples that tea bran has a good control effect on loofah root-knot nematodes.

[0068] Experimental Example 4

[0069] The test variety in this experiment is the highly commercial variety "Chiyu Huaxia" cucumber produced by Tianjin Kerun Cucumber Research Institute. This variety is a strong female cucumber and is widely cultivated and promoted in facility vegetable greenhouses.

[0070] The experiment was carried out on April 1, 2024 in the standardized vegetable greenhouse of Wanxing Experimental Base of the National Vegetable Quality Standard Center (Ganzhou), with a plant spacing of 25 cm, a ridge spacing of 140 cm, and single-row planting. The experiment was divided into two groups, the experimental group and the control group. The plot area of ​​the experimental group and the control group was 208m 2 (26.0m×8.0m), 1.5m wide protective rows were set around the plot, and the organic fertilizer in Example 4 was evenly spread during the land preparation and ridge making in the experimental group. The spreading rate was 1 ton / 667m 2 The control group was fertilized with the organic fertilizer in Example 1, with the same amount as the test group, and thiazothion was drip-irrigated for root-knot nematode control 20 days after planting. Each group was set up with 4 replicates, a total of 8 plots, and randomized block arrangement. The product harvest period is from April 30, 2024 to June 15, 2024.

[0071] The results showed that the leaves were obviously yellowish and the growth was weak 20 days after planting. After drip irrigation with thiazothiazide, the root-knot nematodes were effectively suppressed, but obvious root-knot nematode damage still occurred in the third month; while the application of relevant organic fertilizers before planting did not find any disease until the seedlings were pulled out ( Figure 5 , 6 The yield measurement results showed that the experimental group yield was 4193.20kg / 667m 2 The control group's equivalent yield was 2541.64kg / 667m 2 , the yield decreased by 39.38%. From the above examples, it can be seen that the oil tea bran has a good control effect on cucumber root knot nematodes.

[0072] From the above examples, it can be seen that the present invention provides a tea bran organic fertilizer and a preparation method and application thereof. Adding tea bran to the raw materials used in preparing organic fertilizer can effectively reduce the number of root knots of cowpea, loofah and cucumber, thereby facilitating the growth of vegetables and increasing the yield.

[0073] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A method for preparing tea bran organic fertilizer, characterized in that: The following steps are involved: (1) mixing plant-derived organic waste, tea bran and livestock manure, adjusting the moisture content of the mixture to between 50% and 65%, adding a microbial agent, and performing high-temperature aerobic fermentation after compaction to obtain a fermented material after 20 to 25 days; (2) Piling the fermentation material in step (1) into a pile for anaerobic low-temperature fermentation, and completing the fermentation in 30 to 35 days; (3) drying the fermented material until the moisture content is ≤10%, and then granulating the material to obtain the organic fertilizer.

2. The preparation method according to claim 1, characterized in that: The mass ratio of the livestock excrement, tea bran and plant-based organic waste is 50-60:20-25:15-20.

3. The preparation method according to claim 2, characterized in that: The plant-derived organic waste includes one or more of wheat, rice, corn, sorghum and cotton straw, rice husk, corn cob, bagasse, beet pulp and fruit husk.

4. The preparation method according to claim 3, characterized in that: The carbon-nitrogen ratio of the mixed material is 20-35:1, and the pH value is 5.5-8.

5.

5. The preparation method according to claim 4, characterized in that: The added amount of the microbial agent is 1 to 2‰ of the mass of the mixed material.

6. The preparation method according to claim 5, characterized in that: During the high-temperature aerobic fermentation stage, oxygen is supplied by turning the pile when the temperature is below 55°C, and the temperature is lowered by turning the pile when the temperature is above 65°C.

7. Camellia oleifera bran organic fertilizer prepared according to the preparation method according to any one of claims 1 to 6.

8. Application of the oil-tea camellia bran organic fertilizer according to claim 7 in at least one of the following: (1) Application in the prevention and treatment of root-knot nematodes in cucurbitaceae and leguminous plants; (2) Application in promoting plant growth and / or increasing yield.

9. A method for controlling root-knot nematodes in crops, characterized in that: Spread the oil-tea bran organic fertilizer according to claim 7 before planting crops.

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