Nano-enzyme-containing washing powder and preparation method thereof

Abstract

The invention belongs to the field of daily chemical products, and discloses washing powder containing nano-enzyme and a preparation method of the washing powder. The manganese selenide nano-enzyme has activity of simulated protease, lipase, glycosidase and oxidases. The manganese selenide nano enzyme can effectively improve the enzyme activity of the washing powder for a long time in a washing powder environment, and meanwhile, other enzyme stabilizers do not need to be added. Compared with commercially available washing powder (Otmian, Lianbai, Anli and the like), the washing powder provided by the invention has more efficient decontamination capability and sterilization capability, the content of total carbon, total phosphorus and total nitrogen in used water is low, and the washing powder has environmental protection benefits.

Description

Technical Field

[0001] The invention relates to a washing powder containing nanoenzyme and a preparation method thereof, belonging to the field of daily chemical products. Background Art

[0002] Biological enzymes are commonly used additives for washing powders on the market. Currently, the commonly used biological enzymes include proteases, lipases, amylases, and cellulases. Proteases can first break down proteins into soluble peptide bonds, and then break them down into amino acids, so that they can be easily washed away. Amylases can hydrolyze starch into dextrin or maltose, thereby enhancing the decontamination effect. For example, a patent (publication number: CN 113969219 A) has invented an enzyme laundry detergent with added wheat protease to enhance the decontamination effect; a patent (publication number: CN 114621829A) adds lysozyme to laundry detergent to enhance the antibacterial effect of the laundry detergent.

[0003] However, traditional biological enzymes have the disadvantages of being easily inactivated at high temperatures, poor stability, and weak catalytic ability, which limits the effect of biological enzymes in detergents. The patent (publication number: CN 111484901 A) encapsulates the easily inactivated enzyme preparation in the three-dimensional network structure of biological cellulose gel, which can effectively maintain or improve the enzyme activity of the laundry detergent in the liquid environment for a long time, but the cost of this method is relatively high.

[0004] Nanozymes are a type of nanobiomaterials with properties similar to natural enzymes. They have the advantages of good biocompatibility, mild reaction, good stability, easy storage, and environmental friendliness. Manganese selenide nanozymes can catalyze the hydrolysis of proteins, fats, polysaccharides, etc. under mild conditions and degrade biomacromolecules. Manganese selenide nanozymes can also produce reactive oxygen species, destroy bacterial cell membranes, proteins, nucleic acids and other biomolecules, leading to bacterial death. At the same time, they inhibit the activity of some key enzymes in bacteria, interfere with the energy metabolism of bacteria, induce bacterial cell apoptosis and cause programmed cell death, thereby achieving a bactericidal effect. In addition, manganese selenide nanozymes do not produce toxic and harmful substances during use, do not cause water pollution, and are environmentally friendly. However, nanozymes have not yet been used as detergent additives. Summary of the invention

[0005] The present invention provides a selenized manganese nanozyme washing powder and a preparation method thereof. The selenized manganese nanozyme has simulated protease, lipase, glycosidase and oxidase activities, and can effectively remove dirt and fight bacteria. In addition, the selenized manganese nanozyme can maintain or improve the enzyme activity of the washing powder without adding other enzyme stabilizers. To achieve the above purpose, the present invention adopts the following specific scheme to achieve:

[0006] Using a hydrothermal synthesis method, 3 mmol SeO2 and 3 mmol MnCl2·4H2O were first dissolved in 37 ml of deionized water; then about 3 ml of N2H4·H2O (85% concentration) was added to the selenium mixture, and a uniform solution was formed after magnetic stirring for 30 minutes;

[0007] The homogeneous solution was transferred to a Teflon-coated stainless steel autoclave, sealed, and heated at 180 °C for 28 h;

[0008] The obtained solution was centrifuged three times with deionized water and ethanol and dried at 60 °C for 24 h to obtain the final MnSe product.

[0009] The washing powder containing selenized manganese nanozyme of the present invention further contains surfactant (anionic or nonionic surfactant), softener (sodium citrate, sodium metasilicate, etc.), pH regulator (alcoholamine compound, sodium hydroxide, potassium hydroxide, methanesulfonic acid, etc.), cosolvent (ethanol, ethylene glycol, glycerol, etc.), preservative, fragrance, color enhancer, etc. The concentration of active matter in the surfactant is between 15wt% and 40wt%.

[0010] The selenized manganese nanozyme of the present invention can efficiently remove stains and sterilize, and can effectively maintain or improve the enzyme activity of the washing powder for a long time in the washing powder environment, without the need to add other enzyme stabilizers. Tests have shown that the washing powder of the present invention has an enzyme activity loss of no more than 20% when stored for more than 6 months on the shelf; during laundry, the enzyme activity is effectively released for 5-10 minutes; it has good cleaning function, and also has a visual sense of goodwill and recognition for consumers, and is a washing powder product with excellent effect and strong cleaning activity. BRIEF DESCRIPTION OF THE DRAWINGS

[0011] Figure 1 XRD analysis results of manganese selenide nanozyme

[0012] Figure 2 TEM image of manganese selenide nanozyme

[0013] Figure 3 Manganese selenide nanoenzyme laundry detergent

[0014] Figure 4 Decontamination effect comparison chart

[0015] Figure 5 Antibacterial effect comparison chart Specific implementation plan

[0016] In order to enable those skilled in the art to better understand the present invention, the present invention is further described in detail below in conjunction with specific embodiments. It should also be understood that the following examples are only used to further illustrate the present invention and cannot be construed as limiting the scope of protection of the present invention. Some non-essential improvements and adjustments made by those skilled in the art based on the above content of the present invention all belong to the scope of protection of the present invention.

[0017] Example 1: Synthesis of manganese selenide nanozyme

[0018] Using the hydrothermal synthesis method, first 3mmol SeO2 and 3mmol MnCl2·4H2O were dissolved in 37ml deionized water; then about 3ml N2H4·H2O (85% concentration) was added to the selenium mixture, and a uniform solution was formed after magnetic stirring for 30 minutes; the uniform solution was transferred to a Teflon-coated stainless steel autoclave, sealed, and heated at 180°C for 28 hours; the obtained solution was centrifuged three times with deionized water and ethanol, and dried at 60°C for 24 hours to obtain the final product.

[0019] Example 2: Synthesis of manganese selenide nanoenzyme laundry detergent

[0020] The formula of other ingredients of washing powder (percentage by weight): linear alkylbenzene sulfonate sodium: 20%; fatty alcohol polyoxyethylene ether sodium sulfate: 2.5%; fatty alcohol polyoxyethylene ether ammonium sulfate: 2.5%; sodium citrate: 3.5%; citric acid: 0.1%; NaCl: 0.5%; fluorescent brightener: 0.14%; glycerol: 1%; essence (lavender fragrance): 0.1%. Various raw materials are added into a mixer according to the formula ratio for mixing, followed by tableting, granulation, drying and packaging to finally obtain a washing powder product.

[0021] Implementation effect 1: Decontamination ability test of manganese selenide nanoenzyme laundry detergent

[0022] Four towels of the same brand and style were sprinkled with the same amount of cooking oil at the same position to form similar stains; four other towels were sprinkled with the same amount of soy sauce in the same way; each towel was placed in a separate basin, and 100 ml of tap water at room temperature was added to soak for 15 minutes; an equal amount of washing powder containing selenium manganese nanoenzyme and commercial washing powder (Amway, Omo, Liby) were used for control tests, and 10 grams of each washing powder was added to each basin; the stains were rubbed by hand for 10 minutes and then compared; the stain residues of each group of towels were detected, and the residual rate was calculated as follows:

[0023] Detergent / residue rate Grease stains soy sauce Amway 8％ 3％ secret 20％ 11％ Liby 18％ 10％ Manganese selenide nanozyme 5％ 2％

[0024] It can be seen from the above experiments that the washing powder containing selenide manganese nanozyme of the present invention has strong cleaning ability, especially for stains of oil stains and bean stains, and has a very good cleaning effect.

[0025] Implementation effect 2: Antibacterial ability test of manganese selenide nanoenzyme laundry detergent

[0026] Prepare three washing basins, fill them with equal amounts of clean water, and add equal amounts of washing powder containing selenized manganese nanoenzymes or commercially available washing powder (Amway, Omo, Liby) to each washing basin. Put an identical towel in each washing basin to ensure that each piece of cotton cloth has the same soaking time after contact with the laundry detergent. Take out each soaked towel and place it on a flat plate culture dish containing bacterial culture medium, and place the culture dish in a constant temperature incubator. After 24 hours of incubation, observe whether there is bacterial growth in each culture dish.

[0027] Implementation effect 3: Detergent water quality test

[0028] Take the same volume of wastewater from each washing powder basin in Implementation Effect 1, and take the same amount of tap water as a control group; measure the content of N, P, and C in the water to evaluate the impact of the washing powder on water quality. The results are as follows:

[0029] Detergent / Total Content C / mg / L N / mg / L P / mg / L Tap water 2.0 0.8 0.07 Amway 2.5 1.1 0.2 secret 3.1 1.8 0.4 Liby 2.7 1.5 0.7 Manganese selenide nanozyme 2.0 0.8 0.07

[0030] The results show that the manganese selenide nanoenzyme laundry detergent of the present invention is effectively dissolved in water and will be fully rinsed off after the washing process is completed, without causing significant pollution to the environment and water quality. Compared with other commercially available laundry detergents, this product is an environmentally friendly product.

Claims

1. The present invention is a washing powder containing nanoenzyme and a preparation method thereof. The method comprises the following steps: (1) Using the hydrothermal synthesis method, 3 mmol SeO2 and 3 mmol MnCl2·4H2O were first dissolved in 37 ml of deionized water; (2) adding about 3 ml of N2H4·H2O (85% concentration) to the selenium mixture and stirring magnetically for 30 minutes to form a homogeneous solution; (3) transferring the homogeneous solution into a Teflon-coated stainless steel autoclave, sealing it, and heating it at 180° C. for 28 hours; (4) centrifuging the obtained solution with deionized water and ethanol three times, and drying at 60° C. for 24 hours to obtain the final manganese selenide nanozyme product; (5) Prepare 20% of linear alkylbenzene sulfonate sodium; 2.5% of fatty alcohol polyoxyethylene ether sodium sulfate; and 2.5% of fatty alcohol polyoxyethylene ether ammonium sulfate; Sodium citrate: 3.5%; Citric acid: 0.1%; NaCl: 0.5%; Fluorescent whitening agent: 0.14%; Glycerin: 1%; Fragrance (lavender): 0.1%; (6) Various raw materials are added into a mixer according to the formula ratio for mixing, followed by tableting, granulation, drying and packaging to finally obtain a manganese selenide nanoenzyme laundry detergent product.

2. The selenized manganese nanozyme laundry detergent obtained by the preparation method according to claim 1 has simulated protease, lipase, glycosidase and oxidase activities.

3. The manganese selenide nanoenzyme laundry detergent obtained by the preparation method as claimed in claim 1 is an environmentally friendly product.

4. The manganese selenide nanoenzyme laundry detergent obtained by the preparation method as claimed in claim 1 has more efficient decontamination ability and bactericidal effect.

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