Aerobic denitrification thauera LT9 and application thereof

Abstract

The invention relates to an aerobic denitrifying thauera LT9 with the preservation number of CGMCC No.32311. The aerobic denitrifying thauera LT9 can resist antibiotics and aromatic compounds under the condition of low carbon nitrogen ratio (C / N is 2), can realize synchronous degradation of nitrogen and phenol, can be applied to nitrogen removal by denitrification of wastewater in pharmacy, culture, chemical industry and the like, and has practical significance.

Description

Technical Field

[0001] The invention relates to aerobic denitrifying Tauella bacteria LT9 and application thereof. Background Art

[0002] The large amount of wastewater generated by the pharmaceutical industry, aquaculture, and chemical industry contains not only a large amount of nitrogen, but also organic pollutants such as antibiotics, phenol, aniline, and bisphenol A. Among them, excessive nitrogen discharge deteriorates the quality of water bodies, causing black and smelly water bodies, eutrophication of water bodies, and other problems that threaten the ecological environment of water bodies.

[0003] Traditional biological denitrification includes two steps: aerobic nitrification and anaerobic denitrification, which need to be completed in two separate systems, increasing the complexity and operating costs of sewage treatment. The emergence and application of aerobic denitrifying bacteria have broken this limitation, allowing nitrification and denitrification to proceed simultaneously, achieving synchronous denitrification at the microbial level. Using aerobic denitrifying bacteria to achieve efficient denitrification of water bodies under low carbon-nitrogen ratio conditions has become a current research hotspot.

[0004] In addition, organic compounds such as antibiotics, phenol, aniline, bisphenol A, etc. contained in wastewater are extremely detrimental to the growth of microorganisms. In addition, the presence of antibiotics will also kill or inhibit the growth and reproduction of certain microorganisms, affecting the aerobic denitrification in biological denitrification. At present, there have been studies on aerobic denitrifying bacteria in extreme environments, but there are still few studies on antibiotics, phenol, bisphenol A and aniline. Summary of the invention

[0005] The purpose of the present invention is to provide a strain of Tauesella LT9 which is resistant to aromatic compounds and can perform aerobic denitrification under low carbon-nitrogen ratio conditions and its application.

[0006] The present invention adopts the following technical solution: A species of Tauerella Thauera sp. ) LT9, deposited on October 23, 2024 in the General Microbiology Center of China Culture Collection Administration, located at No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, with the deposit number CGMCCNo.32311.

[0007] Furthermore, the Tauella bacteria LT9 can perform aerobic denitrification under low carbon-nitrogen ratio (C / N=2) conditions.

[0008] Furthermore, the Taueella sp. LT9 may show resistance to penicillin, oxacillin, cephalexin, cefazolin, cefuroxime sodium, kanamycin, erythromycin, lincomycin, co-trimoxazole, clindamycin, and metronidazole.

[0009] Furthermore, the Tauella sp. LT9 can tolerate antibiotics and achieve efficient denitrification.

[0010] Furthermore, the Tauella sp. LT9 can tolerate and degrade phenol and achieve aerobic denitrification.

[0011] Furthermore, the Tauella sp. LT9 can tolerate bisphenol A and achieve efficient denitrification.

[0012] Furthermore, the Tauella sp. LT9 can tolerate aniline and achieve efficient denitrification.

[0013] An application of the above-mentioned Tauella sp. LT9 in sewage treatment.

[0014] An application of the above-mentioned Tauella sp. LT9 in the treatment of nitrogen-containing alkaline sewage.

[0015] An application of the above-mentioned Tauella sp. LT9 in the treatment of alkaline antibiotic wastewater containing nitrogen and aromatic compounds.

[0016] A sewage treatment agent comprising the above-mentioned Tauella sp. LT9.

[0017] The beneficial effects of the present invention are as follows: the Tauella LT9 of the present invention is an aerobic denitrifying bacterium, which can resist antibiotics and aromatic compounds (phenol, aniline and bisphenol A) under low carbon-nitrogen ratio conditions, and can achieve simultaneous degradation of nitrogen and phenol. The use of the Tauella LT9 of the present invention has practical significance for the treatment of wastewater in the pharmaceutical industry, aquaculture, chemical industry and the like. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 This is a scanning electron micrograph of strain LT9.

[0019] Figure 2 is the phylogenetic tree of strain LT9.

[0020] Figure 3 The denitrification characteristics of strain LT9 under different nitrogen source backgrounds when C / N is 2.

[0021] Figure 4 The effects of different factors on the denitrification characteristics of strain LT9.

[0022] Figure 5 This is the plate image of the drug sensitivity test of strain LT9.

[0023] Figure 6 The denitrification characteristics of strain LT9 under the influence of different concentrations of antibiotics.

[0024] Figure 7 The nitrate nitrogen removal rate and phenol degradation rate of strain LT9 under the phenol background.

[0025] Figure 8 is the denitrification efficiency of strain LT9 in the bisphenol A background.

[0026] Fig. 9 is the denitrification efficiency of strain LT9 in the aniline background. DETAILED DESCRIPTION

[0027] Example 1 Isolation and screening of strains (1) Enrichment: Fresh activated sludge samples were collected from a pharmaceutical wastewater treatment plant in Shijiazhuang, Hebei Province. 10 mL of fresh activated sludge was added to 100 mL of enrichment medium (LB medium) and cultured at 25 °C and 120 rpm with constant temperature shaking. The enrichment medium was replaced every 2 days, and the process was repeated twice.

[0028] (2) Acclimation: 5% (v / v) enriched bacterial suspension was transferred to a 250-mL conical flask containing 150 mL of denitrification medium and cultured at 25 °C and 120 rpm with constant temperature shaking. During the acclimation process, raw pharmaceutical wastewater was continuously added in a ratio (volume ratio) of 10 / 0, 9 / 1, 8 / 2, 7 / 3, 6 / 4, 5 / 5, 4 / 6, 3 / 7, and 2 / 8 of culture medium to raw pharmaceutical wastewater. Each concentration of culture medium was cultured for 3 days and repeated twice. The acclimation process took about 54 days.

[0029] (3) Isolation and purification: Take 1 mL to 9 mL of the bacterial suspension after acclimation and add them to the PBS buffer solution according to 10 -1 , 10 -2 , 10 -3 , 10 -4 , 10 -5 , 10 -6 , and then dilute the mixture in multiples of , and transfer 200 μL of the mixed solution of different concentrations to the surface of the solid denitrification medium in a sterile clean bench. Use a sterile glass coating rod to evenly spread the bacterial solution on the plate. After the bacterial solution penetrates into the solid medium, seal it and invert it in a 25°C constant temperature incubator for 2 days. When clear single colonies grow, screen colonies of different shapes and sizes and streak them on the surface of the solid denitrification medium. After isolating a single colony, repeat the streak at least twice for purification, and obtain the purified single strain, which is placed in a 4°C refrigerator for screening.

[0030] (4) Screening: The isolated single strains were inoculated into a 250 mL conical flask containing 100 mL of enrichment medium (LB medium) for enrichment culture for 2 days. After the culture was completed, the bacterial solution was poured into a sterilized 50 mL centrifuge tube and centrifuged at 6000 r / min for 5 minutes. The supernatant was poured out and the bacteria in the tube were mixed with PBS buffer to make a bacterial suspension. 10 mL of the mixed solution was inoculated into 150 mL of denitrification medium for culture for 3 days, and the nitrate nitrogen removal efficiency was measured. The strains with a nitrate nitrogen removal efficiency of more than 90% were screened, and the mixed bacterial solution was inoculated again into 150 mL of denitrification culture medium to verify the nitrate nitrogen removal efficiency. This process was repeated at least 3 times to select nitrate nitrogen (NO3 - The strain with a removal rate of -N) maintained above 90% was obtained as strain LT9.

[0031] The above culture medium includes: Enrichment medium (LB medium): tryptone 10 g / L, yeast extract powder 5 g / L, NaCl 10 g / L, pH 7.2, sterilization at 121°C for 30 min.

[0032] Denitrification medium: CH3COONa 4.69 g / L, KNO3 0.722 g / L, Wei's salt solution (including K2HPO4·3H2O 0.75 g / L, NaH2PO4·2H2O 0.25 g / L, NaCl 0.12 g / L, MnSO4·H2O 0.01 g / L, MgSO4·7H2O 0.05 g / L, FeSO4·7H2O 0.01 g / L), high pressure sterilization at 121°C for 30 min.

[0033] Solid denitrification medium: CH3COONa 4.69g / L, KNO3 0.722g / L, K2HPO4·3H2O 0.75g / L, NaH2PO4·2H2O 0.25g / L, NaCl 0.12g / L, MnSO4·H2O 0.01g / L, MgSO4·7H2O 0.05g / L, FeSO4·7H2O 0.01g / L, agar 20g / L, high pressure sterilization at 121℃ for 30min.

[0034] PBS buffer: NaCl 8 g / L, KCl 0.2 g / L, Na2HPO4·12H2O 3.63 g / L, KH2PO4 0.24 g / L, pH adjusted to 7.4.

[0035] Example 2 Morphological and molecular identification of strain LT9 (1) Morphological characteristics: Take 50 mL of the activated bacterial suspension of strain LT9 and put it into a 50 mL centrifuge tube. Centrifuge at 6000 r / min for 5 min. Discard the supernatant. After the bacterial precipitate is fixed, washed, dehydrated, freeze-dried, and gold-sprayed, the strain LT9 bacteria are observed to be rod-shaped under an electron scanning microscope.

[0036] (2) Molecular identification: The genomic DNA of the strain was extracted and the PCR product was amplified using the universal primers 27F / 1492R for bacterial 16S rRNA gene amplification. The product was sent to Shanghai Meiji Biotechnology Co., Ltd. for sequence sequencing and compared with the NCBI database to identify the species relationship. It was found that strain LT9 was similar to Thauera sp. The distance was the closest and the similarity was the highest (99.22%). Based on the above analysis, strain LT9 was identified as Taue Thauera sp. The phylogenetic tree of strain LT9 was constructed using MEGA11 using the neighbor-joining method. Figure 2 shown.

[0037] The Taueella Thauera sp. ) LT9 was sent to the General Microbiology Center of China Microorganism Culture Collection Administration for preservation on October 23, 2024. The address is No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No.32311.

[0038] Example 3 Denitrification characteristics of Tauella sinensis LT9 under different nitrogen source backgrounds at low carbon-nitrogen ratio (C / N=2) The activated bacterial suspension of Taueella LT9 was inoculated into denitrification medium with a C / N ratio of 2 and nitrate nitrogen, nitrite nitrogen, and ammonia nitrogen as single nitrogen sources, nitrate nitrogen and ammonia nitrogen, and nitrite nitrogen and ammonia nitrogen as mixed nitrogen sources at a 5% (v / v) inoculation rate. The culture was kept constant at 25°C and 120 rpm for 72 h, and samples were taken to measure the OD 600 , nitrate nitrogen, ammonia nitrogen, nitrite nitrogen and total dissolved nitrogen.

[0039] The denitrification culture medium with nitrate nitrogen as the single nitrogen source is: CH3COONa 0.6833g / L, KNO3 0.722g / L, and Weiss salt solution (including K2HPO4·3H2O 0.75g / L, NaH2PO4·2H2O 0.25g / L, NaCl 0.12g / L, MnSO4·H2O 0.01g / L, MgSO4·7H2O 0.05g / L, and FeSO4·7H2O 0.01g / L).

[0040] The culture medium with nitrite nitrogen as the single nitrogen source is: CH3COONa 0.6833 g / L, NaNO2 0.4929 g / L, and Weiss salt solution (including K2HPO4·3H2O 0.75 g / L, NaH2PO4·2H2O 0.25 g / L, NaCl 0.12 g / L, MnSO4·H2O 0.01 g / L, MgSO4·7H2O 0.05 g / L, and FeSO4·7H2O 0.01 g / L).

[0041] The culture medium with ammonia nitrogen as the single nitrogen source is: CH3COONa 0.6833 g / L, (NH4)2SO4 0.472 g / L, and Weiss salt solution (including K2HPO4·3H2O 0.75 g / L, NaH2PO4·2H2O 0.25 g / L, NaCl 0.12 g / L, MnSO4·H2O 0.01 g / L, MgSO4·7H2O 0.05 g / L, and FeSO4·7H2O 0.01 g / L).

[0042] The culture medium with nitrate nitrogen and ammonia nitrogen as mixed nitrogen sources is: CH3COONa 0.6833 g / L, KNO3 0.361 g / L, (NH4)2SO4 0.236 g / L, and Vickers salt solution (including K2HPO4·3H2O 0.75 g / L, NaH2PO4·2H2O 0.25 g / L, NaCl 0.12 g / L, MnSO4·H2O 0.01 g / L, MgSO4·7H2O 0.05 g / L, and FeSO4·7H2O 0.01 g / L).

[0043] The culture medium with nitrite nitrogen and ammonia nitrogen as mixed nitrogen sources is: CH3COONa 0.6833 g / L, NaNO2 0.2465 g / L, (NH4)2SO4 0.236 g / L, and Weiss salt solution (including K2HPO4·3H2O 0.75 g / L, NaH2PO4·2H2O 0.25 g / L, NaCl 0.12 g / L, MnSO4·H2O 0.01 g / L, MgSO4·7H2O 0.05 g / L, and FeSO4·7H2O 0.01 g / L).

[0044] The results are as follows Figure 3 As shown in the figure, when nitrate nitrogen was used as the sole nitrogen source, strain LT9 had a - -N and TDN removal rates were 99.70% and 97.98% respectively; when nitrite nitrogen was used as the sole nitrogen source, the removal of NO2 - -N and TDN removal rates were 89.59% and 79.53% respectively; when ammonia nitrogen was the only nitrogen source, the removal of NH4 +-N and TDN removal rates were 37.02% and 32.21% respectively. When nitrate nitrogen and ammonia nitrogen were used as mixed nitrogen sources, strain LT9 had a significant effect on NO3 - -N、NH4 + The removal rates of -N and TDN were 98.61%, 31.52% and 63.97% respectively; when nitrite nitrogen and ammonia nitrogen were used as mixed nitrogen sources, the removal of NO2 - -N、NH4 + The removal rates of -N and TDN were 99.73%, 18.67% and 52.86%, respectively. The above results show that strain LT9 can effectively utilize nitrate nitrogen and nitrite nitrogen, but has a low utilization rate for ammonia nitrogen, that is, it can aerobically denitrify and achieve efficient nitrogen removal under low carbon-nitrogen ratio conditions.

[0045] Example 4 Effects of different factors on the denitrification characteristics of Tauella LT9 (1) Carbon-nitrogen ratio KNO3 was selected as the single nitrogen source (the components were the same as those in Example 3), and the C / N of the denitrification medium was adjusted to 2, 4, 6, 8, and 10 by adjusting the content of sodium acetate. The effect of different C / N conditions on the denitrification characteristics of strain LT9 was investigated. The bacterial suspension of Tauesella LT9 in the logarithmic growth phase was inoculated into denitrification medium with different C / N at an inoculation rate of 5% (v / v), and cultured at 25°C and 120rpm for 48 hours. Samples were taken to measure the OD 600 The samples were filtered through a 0.45 μm filter membrane and then the nitrate nitrogen, ammonia nitrogen, nitrite nitrogen and total dissolved nitrogen were determined.

[0046] The results are as follows Figure 4 As shown in a, strain LT9 can effectively remove nitrogen in the range of C / N of 2-10, and has a strong inhibitory effect on NO3 - The removal rates of -N and total dissolved nitrogen (TDN) were both above 95%, indicating that strain LT9 can adapt to low C / N conditions and achieve efficient denitrification.

[0047] (2) Carbon source Nitrate nitrogen was used as the sole nitrogen source, and the C / N ratio was determined to be 2. Sodium acetate, sodium succinate, sodium citrate, and sucrose were used as the sole carbon sources in the denitrification medium. The activated bacterial suspension of Tauesella LT9 was inoculated into four denitrification mediums with different carbon sources at an inoculation rate of 5% (v / v) (except for the carbon source, the other components were the same as in Example 3), and cultured at 25°C and 120 rpm for 48 h. Samples were taken to measure the OD 600 , nitrate nitrogen, ammonia nitrogen, nitrite nitrogen and total dissolved nitrogen.

[0048] The results are as follows Figure 4 As shown in b, strain LT9 had the best effect when sodium acetate was used as the sole carbon source. At 48 h, NO3- The removal rates of -N and TDN were 99.89% and 98.44%, respectively; however, the denitrification effect was not obvious when sodium succinate, sodium citrate and sucrose were used as the only carbon source, and the accumulation of nitrite was relatively high.

[0049] (3) pH Nitrate nitrogen was used as the sole nitrogen source, C / N was determined to be 2, sodium acetate was used as the sole carbon source, and the initial pH of the denitrification medium was adjusted to 7, 8, 9, 10, 11, and 12 using 1 mol / L HCl and 1 mol / L NaOH, and the bacterial suspension of Tauesella LT9 in the logarithmic growth phase was inoculated into denitrification medium with different pH values ​​(the same components as in Example 3) at an inoculum amount of 5% (v / v), and cultured at 25°C and 120 rpm for 72 h, and samples were taken to measure OD 600 , nitrate nitrogen, ammonia nitrogen, nitrite nitrogen and total dissolved nitrogen. Results Figure 4 c, strain LT9 at an initial pH of 8-11 to NO3 - The removal rates of -N and TDN were both above 95%, and there was almost no accumulation of nitrite. At pH 7, the removal of NO3 by strain LT9 was - The removal rates of -N and TDN were 80.39% and 78.17%, respectively, indicating that strain LT9 was more suitable for growth and denitrification under slightly alkaline and alkaline conditions.

[0050] In summary, the optimal growth and denitrification conditions of strain LT9 were determined through the influencing factor experiment as follows: C / N was 2, the carbon source was sodium acetate, and the pH was 8-11.

[0051] Example 5 Drug sensitivity test of Taueella LT9 The paper diffusion method was used to conduct a drug sensitivity test on Tauerella LT9. In the clean bench, 0.2 mL of the bacterial solution of Tauerella LT9 after culturing in a denitrification medium (same as Example 3, nitrate nitrogen as the sole nitrogen source) for 24 hours was evenly coated on the surface of the LB solid medium, and allowed to stand for 2 to 3 minutes until the bacterial solution dried. The drug-sensitive paper was attached to the surface of the plate with sterile tweezers and the paper was gently pressed to fit tightly. After sealing, it was inverted and cultured in a 35°C constant temperature incubator for 18 to 24 hours. Three parallel samples were set for each group of experiments. After the culture was completed, the diameter of the inhibition zone was measured with a caliper, and the results were judged according to the antibiotic sensitivity standards of the Clinical Laboratory Standards Institute (CLSI).

[0052] The results of drug sensitivity test are as follows Figure 5As shown in Table 1 (1-30 are penicillin, oxacillin, ampicillin, piperacillin, cephalexin, cefazolin, cefuroxime sodium, ceftazidime, ceftriaxone, amikacin, gentamicin, kanamycin, streptomycin, tetracycline, minocycline, erythromycin, azithromycin, norfloxacin, ciprofloxacin, lincomycin, polymyxin B, cotrimoxazole, chloramphenicol, clindamycin, levofloxacin, imipenem, doxycycline, chlorfenicol, metronidazole and blank). The specific results of the antibiotic resistance test are shown in Table 1. According to the performance of the strain in the resistance test, it can provide a preliminary indication of the antibiotic resistance of the strain LT9, and it can be used as an indicator organism in aquaculture.

[0053] Table 1 Results of drug sensitivity test of Taueella LT9 .

[0054] Example 6 Denitrification characteristics of Taueella sp. LT9 under the influence of different concentrations of antibiotics The activated bacterial suspension of Tauella LT9 was inoculated into denitrification medium (same as Example 3, nitrate nitrogen as the sole nitrogen source) containing different concentrations of tetracycline (0, 50, 100, 250, 500 mg / L) and different concentrations of sulfamethoxazole (0, 50, 100, 250, 500 mg / L) at an inoculum volume of 5% (v / v), and cultured at 25°C, 120 rpm under constant temperature shaking conditions for 6 days and 3 days, respectively, and samples were taken at regular intervals to measure OD 600 , nitrate nitrogen, ammonia nitrogen, nitrite nitrogen and total dissolved nitrogen.

[0055] The results are as follows Figure 6 Tetracycline has a significant effect on the denitrification efficiency of LT9. The strain has a significant effect on NO3 - The removal rates of -N and TDN reached over 95%; the higher the tetracycline concentration, the longer the adaptation time required for the growth and denitrification of strain LT9; sulfamethoxazole in the range of 500 μg / L had almost no effect on strain LT9, and compared with the control group without the addition of antibiotics, the addition of sulfamethoxazole did not inhibit the denitrification efficiency of the strain.

[0056] Example 7 Denitrification performance and phenol degradation performance of Tauerella LT9 in the context of phenol The activated bacterial suspension of Tauella LT9 was inoculated into fresh denitrification medium (same as Example 3, nitrate nitrogen as the sole nitrogen source) with different concentrations of phenol (0, 10, 50, 100, 250, 500 mg / L) at a 5% (v / v) inoculation amount, and cultured at 25°C, 120 rpm for 72 h. Samples were taken to measure OD 600 , nitrate nitrogen and phenol, the results are as follows Figure 7 shown.

[0057] With the increase of phenol concentration, the denitrification efficiency of the strain was gradually inhibited, but it was still able to achieve a high removal rate. When 0, 10, 50, 100, 250, and 500 mg / L phenol was added, the nitrate nitrogen removal rates reached 82.34%, 81.02%, 74.06%, 86.57%, 68.68%, and 89.57%, respectively. At the same time, when 10, 50, 100, 250, and 500 mg / L phenol was added, the phenol removal rates reached 99.63%, 99.93%, 99.96%, 99.99%, and 99.99%, respectively, when cultured for 72 hours. This shows that strain LT9 can not only tolerate the influence of phenol to achieve aerobic denitrification, but also achieve the simultaneous degradation of nitrogen and phenol during the culture process.

[0058] Example 8 Denitrification Performance of S. tauei LT9 in the Background of Bisphenol A The bacterial suspension of Tauella tenuissima LT9 in the logarithmic growth phase was inoculated into denitrification medium (same as Example 3, nitrate nitrogen as the sole nitrogen source) with different concentrations of bisphenol A (0, 50, 100, 250 mg / L) at a 5% (v / v) inoculation amount, and cultured at 25°C, 120 rpm for 72 h. Samples were taken to measure OD 600 , nitrate nitrogen, ammonia nitrogen, nitrite nitrogen and total dissolved nitrogen. The results are as follows Figure 8 As the concentration of bisphenol A increases, the denitrification efficiency of the strain is gradually inhibited, but when the concentration of bisphenol A is 50 mg / L, strain LT9 can still remove 54.22% of NO3 - -N and 22.95% TDN, while the concentration of bisphenol A in industrial wastewater was about 10 mg / L, indicating that the strain could tolerate higher concentrations of bisphenol A to achieve efficient denitrification.

[0059] Example 9 Denitrification performance of Tauerella LT9 in the context of aniline The bacterial suspension of Tauella LT9 in the logarithmic growth phase was inoculated into denitrification medium with different concentrations of aniline (0, 10, 50, 100, 250, 500, 1000, 1500, 2000 mg / L) at a 5% (v / v) inoculation rate. The culture was kept at 25°C and 120 rpm for 72 h. Samples were taken to measure the OD 600 , nitrate nitrogen, the results are as follows Fig. 9 The strain LT9 can adapt well to the environment containing aniline substances. It can tolerate aniline within the range of 2000 mg / L for 48 hours and can almost completely remove all nitrate nitrogen, with a removal rate of 90%.

Claims

1. A species of Tauerella Thauera sp. ) LT9, characterized in that, The deposit number is CGMCC No.32311.

2. The Tauella LT9 according to claim 1, characterized in that It can carry out aerobic denitrification under the condition of C / N of 2.

3. The Tauella sp. LT9 according to claim 1, characterized in that It showed resistance to penicillin, oxacillin, cephalexin, cefazolin, cefuroxime sodium, kanamycin, erythromycin, lincomycin, co-trimoxazole, clindamycin, and metronidazole.

4. The Tauella LT9 according to claim 1, characterized in that It tolerates antibiotics and achieves efficient nitrogen removal.

5. The Tauella sp. LT9 according to claim 1, characterized in that It can tolerate and degrade phenol and achieve aerobic denitrification.

6. The Tauella sp. LT9 according to claim 1, characterized in that It is resistant to bisphenol A or aniline and achieves efficient nitrogen removal.

7. Use of the Tauella sp. LT9 as claimed in claim 1 in sewage treatment.

8. Use of the Tauella sp. LT9 as claimed in claim 1 in the treatment of nitrogen-containing alkaline sewage.

9. Use of the Tauella sp. LT9 as claimed in claim 1 in the treatment of alkaline antibiotic wastewater containing nitrogen and aromatic compounds.

10. A sewage treatment agent comprising the Tauella sp. LT9 according to claim 1.

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