Method for improving stress resistance of plants

Abstract

The invention discloses a method for improving stress resistance of plants, and belongs to the technical field of microorganisms for agriculture and forestry. The invention discloses a microbial agent for improving waterlogging tolerance of plants. The microbial agent is prepared from the following components in parts by weight: 20-30 parts of denitrifying bacteria, 15-20 parts of sulfate reducing bacteria, 7-10 parts of special methanogenic bacteria and 3-5 parts of candida utilis. The microbial agent provided by the invention can relieve adverse effects of waterlogging stress on normal growth and development of plants, can significantly improve waterlogging tolerance of the plants and increase the survival rate of the plants, and has wide application value in agriculture and forestry production.

Description

Technical Field

[0001] The invention relates to the technical field of microorganisms for agriculture and forestry, and in particular to a method for improving plant stress resistance. Background Art

[0002] With global climate change, the frequency and intensity of extreme weather events such as floods are increasing year by year, posing a serious threat to agricultural and forestry production.

[0003] Plant waterlogging generally has two meanings: moisture damage and waterlogging. Moisture damage refers to soil that is too wet and saturated with water. The soil water content exceeds the maximum water holding capacity of the soil, and the roots grow in swampy mud; waterlogging refers to the damage to plants when the ground is flooded and all or part of the crops are submerged. In fact, there is no substantial difference between moisture damage and waterlogging in causing physical and chemical changes in the rhizosphere of plants. Plants grow for a longer period of time in waterlogged soil, showing stomata closure, reduced growing leaf area, chlorosis, slowed root growth, and anaerobic respiration that accelerates the accumulation of toxic substances and ultimately leads to root damage and death, which ultimately leads to plant death. The plant planting season is a period of frequent waterlogging and drought, and significant plant losses each year are caused by waterlogging.

[0004] For flooded plants, post-disaster treatment is generally adopted, mainly including removing water from the field, replanting after the disaster, etc., to reduce the impact of the disaster on actual production. However, these treatment methods will cause a lot of manpower and financial losses. Therefore, improving the waterlogging resistance of plants is crucial to agricultural and forestry production. Summary of the invention

[0005] The purpose of the present invention is to provide a method for improving plant stress resistance to solve the problems existing in the above-mentioned prior art.

[0006] To achieve the above object, the present invention provides the following solutions:

[0007] One of the technical solutions of the present invention is a microbial agent for improving plant waterlogging resistance, comprising the following components in parts by weight: 20 to 30 parts of denitrifying bacteria, 15 to 20 parts of sulfate-reducing bacteria, 7 to 10 parts of methanogenic bacteria and 3 to 5 parts of utilis.

[0008] The second technical solution of the present invention is the use of the microbial agent in the preparation of a product for improving plant waterlogging resistance.

[0009] The third technical solution of the present invention is a method for improving plant waterlogging resistance, which comprises applying the microbial agent to plants to improve the survival rate of plants in a waterlogged environment.

[0010] Based on the above technical solution, the present invention has the following technical effects:

[0011] The present invention provides a microbial agent for improving plant waterlogging resistance, which can alleviate the adverse effects of flooding stress on the normal growth and development of plants, can significantly improve the waterlogging resistance of plants, and improve the survival rate of plants, and has broad application value in agricultural and forestry production. DETAILED DESCRIPTION

[0012] Now, various exemplary embodiments of the present invention are described in detail. The methods in the examples are conventional methods unless otherwise specified, and the reagents used are conventional commercially available reagents or reagents prepared by conventional methods unless otherwise specified. This detailed description should not be considered as a limitation of the present invention, but should be understood as a more detailed description of certain aspects, features and embodiments of the present invention.

[0013] It should be understood that the terms described in the present invention are only for describing a particular embodiment and are not intended to limit the present invention. In addition, for the numerical range in the present invention, it should be understood that each intermediate value between the upper and lower limits of the scope is also specifically disclosed. The intermediate value in any stated value or stated range, and each smaller range between any other stated value or intermediate value in the described range is also included in the present invention. The upper and lower limits of these smaller ranges can be independently included or excluded in the scope.

[0014] Unless otherwise indicated, all technical and scientific terms used herein have the same meanings as those generally understood by those skilled in the art. Although the present invention describes only preferred methods and materials, any methods and materials similar or equivalent to those described herein may also be used in the implementation or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials associated with the documents. In the event of a conflict with any incorporated document, the content of this specification shall prevail.

[0015] It will be apparent to those skilled in the art that various modifications and variations may be made to the specific embodiments of the present invention description without departing from the scope or spirit of the present invention. Other embodiments derived from the present invention description will be apparent to the skilled artisan. The present invention description and examples are exemplary only.

[0016] The words “include,” “including,” “have,” “contain,” etc. used in this document are open-ended terms, meaning including but not limited to.

[0017] The technical solutions described in the present invention, unless otherwise specified, are all conventional solutions in the art, and the reagents or raw materials used, unless otherwise specified, are purchased from commercial channels or have been disclosed.

[0018] The embodiment of the present invention provides a microbial agent for improving plant waterlogging resistance, comprising the following components in parts by weight: 20 to 30 parts of denitrifying bacteria, 15 to 20 parts of sulfate-reducing bacteria, 7 to 10 parts of methanogenic bacteria, and 3 to 5 parts of Candida utilis.

[0019] Denitrifying bacteria: Under flooded conditions, denitrifying bacteria help plant roots maintain energy supply under anaerobic conditions by reducing nitrates to nitrogen gas, reducing plant competition for oxygen and potentially having a positive impact on plants by improving the redox status of the soil.

[0020] Sulfate-reducing bacteria: These bacteria obtain energy by reducing sulfate under anaerobic conditions and may help improve the soil environment around plant roots and reduce the accumulation of compounds that are harmful to plant roots.

[0021] Methanogens: Methanogens produce methane through metabolism under strictly anaerobic conditions, a process that may help reduce oxidative stress in plant roots.

[0022] Candida utilis: As a facultative anaerobic yeast, Candida utilis can grow under aerobic and anaerobic conditions and may produce plant-beneficial substances through its metabolic activities, helping plants to better adapt to flooding conditions.

[0023] Denitrifying bacteria, sulfate-reducing bacteria, methanogenic bacteria and utilis can be used together to apply the microbial agent provided by the present invention to plants under waterlogging stress, which can significantly improve the waterlogging resistance of the plants and increase the survival rate.

[0024] The embodiment of the present invention also provides the use of the microbial agent in preparing a product for improving plant waterlogging resistance.

[0025] In some specific embodiments, the plant comprises corn.

[0026] In some specific embodiments, the improving the waterlogging tolerance of plants is to improve the survival rate of plants in a waterlogged environment.

[0027] The embodiment of the present invention also provides a method for improving the waterlogging resistance of plants, wherein the microbial agent is applied to plants to improve the survival rate of plants in a waterlogging environment.

[0028] In some specific embodiments, the microbial agent further comprises a step of activation culture before administration.

[0029] In the examples of the present invention, denitrifying bacteria were purchased from Shanghai Gandu Environmental Engineering Co., Ltd.; methanogenic bacteria were purchased from Guangzhou Qingwo Biotechnology Co., Ltd.; sulfate-reducing bacteria were purchased from Sichuan Zuocheng Technology Co., Ltd.; and Candida utilis were purchased from Hubei Dali Chemical Co., Ltd.

[0030] Example 1

[0031] Select healthy corn seeds of uniform size and germinate them in the dark at 25±2°C for 72 hours. Then transplant the seeds with uniform germination into seedling pots for further cultivation.

[0032] In terms of weight, 20 parts of denitrifying bacteria, 15 parts of sulfate-reducing bacteria, 10 parts of methanogenic bacteria and 4 parts of Candida utilis were weighed and activated: Giltay medium was used for activation culture of denitrifying bacteria, Postgate medium was used for activation culture of sulfate-reducing bacteria and Candida utilis, and ATCC medium was used for activation culture of methanogenic bacteria. The activation culture was carried out for 24 hours, and the bacterial solutions were mixed for later use.

[0033] When the seedlings grow to the two-leaf stage, they are flooded: water is poured into the seedling pots to make the final water level 1 to 2 cm higher than the nutrient soil surface. After watering, the activated mixed bacterial solution is applied to the seedling pots. The stress experiment is carried out for 6 days. During the entire experiment, the water level in the seedling pots is always kept 1 to 2 cm higher than the nutrient soil surface.

[0034] At the same time, a control group without microbial liquid was set up. Except for not applying microbial liquid, other conditions of the control group were exactly the same as those of the experimental group.

[0035] After the stress experiment was completed, the corn seedlings were observed and the survival rate was calculated. The results showed that the survival rate of the corn in the control group was 90.67%, and the corn in the experimental group was completely alive, with a survival rate of 100%. At the same time, some leaves of the corn plants in the control group turned yellow and grew slowly. Compared with the control group, the corn plants in the experimental group grew faster, the leaves were green, and the plants were strong.

[0036] Example 2

[0037] Select healthy corn seeds of uniform size and germinate them in the dark at 25±2°C for 72 hours. Then transplant the seeds with uniform germination into seedling pots for further cultivation.

[0038] 30 parts of denitrifying bacteria, 20 parts of sulfate-reducing bacteria, 7 parts of methanogenic bacteria and 3 parts of Candida utilis were weighed by weight and activated: Giltay medium was used for activation culture of denitrifying bacteria, Postgate medium was used for activation culture of sulfate-reducing bacteria and Candida utilis, and ATCC medium was used for activation culture of methanogenic bacteria. The activation culture was carried out for 24 hours, and the bacterial solutions were mixed for later use.

[0039] When the seedlings grow to the two-leaf stage, they are subjected to flooding stress: water is poured into the seedling pots so that the final water level is in the middle of the plant height. After watering, the activated mixed bacterial solution is applied to the seedling pots. The stress experiment is carried out for 6 days, and the water level in the seedling pots is always kept in the middle of the plant height during the entire experiment.

[0040] At the same time, a control group without microbial liquid was set up. Except for not applying microbial liquid, other conditions of the control group were exactly the same as those of the experimental group.

[0041] After the stress experiment was completed, the state of the corn seedlings was observed and the survival rate was calculated. The results showed that the survival rate of corn in the control group was 76.67%, and the survival rate of corn in the experimental group was 91.13%. The corn plants in the control group were weak and stagnant, while the corn plants in the experimental group grew faster, the leaves rarely turned yellow, and the plants were stronger than those in the control group.

[0042] Example 3

[0043] Select healthy corn seeds of uniform size and germinate them in the dark at 25±2°C for 72 hours. Then transplant the seeds with uniform germination into seedling pots for further cultivation.

[0044] In terms of weight, 26 parts of denitrifying bacteria, 16 parts of sulfate-reducing bacteria, 8 parts of methanogenic bacteria and 5 parts of Candida utilis were weighed and activated: Giltay medium was used for activation culture of denitrifying bacteria, Postgate medium was used for activation culture of sulfate-reducing bacteria and Candida utilis, and ATCC medium was used for activation culture of methanogenic bacteria. The activation culture was carried out for 24 hours, and the bacterial solutions were mixed for later use.

[0045] When the seedlings grow to the two-leaf stage, they are subjected to flooding stress: water is poured into the seedling pot until the final water level completely covers the entire plant, and after watering, the activated mixed bacterial solution is applied to the seedling pot. The stress experiment is carried out for 6 days, and the water level in the seedling pot is always kept at a level that completely covers the entire plant during the entire experiment.

[0046] At the same time, a control group without microbial liquid was set up. Except for not applying microbial liquid, other conditions of the control group were exactly the same as those of the experimental group.

[0047] After the stress experiment was completed, the state of the corn seedlings was observed and the survival rate was calculated. The results showed that the survival rate of corn in the control group was 20.05%, and the survival rate of corn in the experimental group was 83.33%. The corn plants in the control group were weak, their growth stagnated, and their leaves became wilted, and even turned black and rotted; compared with the control group, the overall color of the corn plant leaves in the experimental group was "green + partially yellow-green", and the plants were healthier.

[0048] From the above three embodiments, it can be seen that as the degree of flooding increases, the survival rate of corn plants gradually decreases. Due to the rapid oxygen deficiency in the soil, the soil and anaerobic microorganisms produce many substances that are harmful to plants. These harmful substances have different effects on the normal growth and development of corn at different degrees of flooding. The application of the microbial agent of the present invention can alleviate these effects and significantly improve the survival rate of corn plants under flooding stress.

[0049] Obviously, the above embodiments of the present invention are merely examples for clearly illustrating the present invention, and are not intended to limit the implementation methods of the present invention. For ordinary technical users in the relevant field, other different forms of changes or modifications can be made on the basis of the above description. It is not necessary and impossible to list all the implementation methods here. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the protection scope of the claims of the present invention.

Claims

1. A microbial agent for improving plant waterlogging resistance, characterized in that: The invention comprises the following components in parts by weight: 20-30 parts of denitrifying bacteria, 15-20 parts of sulfate reducing bacteria, 7-10 parts of special methanogenic bacteria and 3-5 parts of utilis.

2. Use of the microbial agent as claimed in claim 1 in the preparation of a product for improving plant waterlogging resistance.

3. The use according to claim 2, characterized in that: The plants include corn.

4. The use according to claim 2, characterized in that: The improving of plant waterlogging resistance is to improve the survival rate of plants in waterlogging environment.

5. A method for improving waterlogging resistance of plants, characterized in that: Applying the microbial agent described in claim 1 to plants can improve the survival rate of plants in waterlogging environments.

6. The method according to claim 5, characterized in that The microbial agent further comprises a step of activation culture before application.