一种检测受者中供者比例的NGS方法
By obtaining mixed cell samples of donor and recipient genotypes, sequencing and analysis are performed, solving the problems of cumbersome operation and high cost in existing technologies, achieving more accurate detection of donor DNA content, and reducing detection costs.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- WUHAN KANGSHENGDA MEDICAL LAB CO LTD
- Filing Date
- 2024-12-24
- Publication Date
- 2026-07-17
AI Technical Summary
In existing technologies, when detecting ddcfDNA content in the context of receptor cfDNA, sequencing analysis of receptor gDNA and cfDNA is required, which is cumbersome and costly. If receptor genomic information is not required, the accuracy of the detection will be insufficient.
By obtaining mixed cell samples of donor and recipient genotypes, sequencing is performed to obtain homozygous and heterozygous gene combinations. Multiple SNP and InDel loci are counted, and the donor ratio is analyzed using probability density maps and Gaussian mixture models, thereby reducing the amount of testing data and lowering costs.
By reducing testing data and lowering costs, and by analyzing the genetic combinations of donors and recipients, the accuracy of donor DNA content information is improved, providing more comprehensive molecular genetic evidence.
Smart Images
Figure CN119799864B_ABST