Air tightness detection method and fluid system

Abstract

The invention discloses an air tightness detection method and a fluid system. The method comprises the steps that the fluid system is provided and comprises a pipeline consumable with a working pipeline, a power part, a first pressure sensor and a second pressure sensor, the power part, the first pressure sensor and the second pressure sensor are all installed on the working pipeline, and the first pressure sensor and the second pressure sensor are located on the first side and the second side of the power part respectively; the power part is started, so that gas in the working pipeline flows in the direction from the first side to the second side; the power part is closed, and in a first time period after the power part is closed, a first actual pressure value in the working pipeline located on the first side is measured through the first pressure sensor, and a second actual pressure value in the working pipeline located on the second side is measured through the second pressure sensor; and primarily judging the air tightness of the pipeline consumables by combining the first actual pressure value and the second actual pressure value. Through cooperation of the power piece, the valve assembly and the pressure sensors, whether the air tightness of the pipeline consumables is qualified or not can be automatically detected.

Description

Technical Field

[0001] The present application relates to the field of air tightness detection, and in particular to an air tightness detection method and a fluid system. Background Art

[0002] Air tightness testing is mainly used to test the sealing and air tightness of the piping systems of various industrial products, components or systems, which may include automotive parts, medical devices, home appliances, etc. Air tightness testing can prevent leakage, pollution, energy waste and other problems, and is crucial to ensuring product quality and safety.

[0003] For example, the liquid circuit system of the cell sorting equipment is an indispensable condition to ensure that the cell processing is in a sterile environment. The liquid circuit system contains multiple pipelines. The liquid in the liquid bag used in cell sorting can flow to the required pipeline port through the liquid circuit system. Each pipeline port is controlled by a control valve, and the pipelines surrounded by each control valve form a liquid circuit system. If a leak occurs in the liquid circuit system, the requirements of full closure and sterility in the cell processing process cannot be guaranteed, affecting the cell sorting results. Moreover, if a leak occurs in the liquid circuit system, it may also contaminate the instrument and laboratory environment, causing fire hazards and biochemical pollution risks. Summary of the invention

[0004] In order to solve the above shortcomings, it is necessary to provide an airtightness detection method and a fluid system using the above detection method.

[0005] The first aspect of the present application provides an airtightness detection method for a fluid system. The airtightness detection method includes: providing the fluid system, the fluid system includes a pipeline consumable, a power part, a first pressure sensor and a second pressure sensor, the pipeline consumable includes a working pipeline, the power part, the first pressure sensor and the second pressure sensor are installed on the working pipeline, and the first pressure sensor and the second pressure sensor are respectively located on the first side and the second side of the power part; opening the power part so that the gas in the working pipeline flows from the first side to the second side; closing the power part, and within a first time period after closing the power part, measuring the first actual pressure value in the working pipeline on the first side through the first pressure sensor, and measuring the second actual pressure value in the working pipeline on the second side through the second pressure sensor; combining the first actual pressure value and the second actual pressure value to initially judge the airtightness of the pipeline consumable.

[0006] In some possible implementations, the air tightness detection method also includes: reopening the power component so that the gas in the working pipeline flows in the direction from the second side to the first side; closing the power component, and within a first time period after closing the power component, measuring a third actual pressure value in the working pipeline located on the first side by the first pressure sensor, and measuring a fourth actual pressure value in the working pipeline located on the second side by the second pressure sensor; and judging the air tightness of the pipeline consumables by combining the third actual pressure value and the fourth actual pressure value.

[0007] In some possible implementations, before turning on the power component, the air tightness detection method also includes: measuring a first empty pipe pressure value in the working pipeline located on the first side by the first pressure sensor, and measuring a second empty pipe pressure value in the working pipeline located on the second side by the second pressure sensor; within a first time period after closing the power component, measuring a first measured pressure value in the working pipeline located on the first side by the first pressure sensor, and measuring a second measured pressure value in the working pipeline located on the second side by the second pressure sensor; calculating the first actual pressure value according to the difference between the first measured pressure value and the first empty pipe pressure value, and calculating the second actual pressure value according to the difference between the second measured pressure value and the second empty pipe pressure value.

[0008] In some possible implementations, the first measured pressure value is a plurality of first measured pressure values ​​continuously measured within a first time period after the power component is turned off, the second measured pressure value is a plurality of second measured pressure values ​​continuously measured within a first time period after the power component is turned off, the first actual pressure value is the difference between each of the first measured pressure values ​​and the first empty pipe pressure value, and the second actual pressure value is the difference between each of the second measured pressure values ​​and the second empty pipe pressure value. When the first actual pressure value and the second actual pressure value meet a predetermined condition, the air tightness of the pipeline consumable is initially judged to be qualified, and the predetermined condition includes: each of the first actual pressure values ​​is less than the first empty pipe pressure value, and each of the second actual pressure values ​​is greater than the second empty pipe pressure value; the range of the absolute values ​​of the plurality of the first actual pressure values ​​does not exceed 10% of the maximum value among the absolute values ​​of the plurality of the first actual pressure values, and the range of the absolute values ​​of the plurality of the second actual pressure values ​​does not exceed 10% of the maximum value among the absolute values ​​of the plurality of the second actual pressure values.

[0009] In some possible implementations, after the power part is turned off, the airtightness detection method further includes: in a second time period after the first time period, continuously measuring multiple third measured pressure values ​​in the working pipeline on the first side by the first pressure sensor, and continuously measuring multiple fourth measured pressure values ​​in the working pipeline on the second side by the second pressure sensor; calculating the average value of the multiple first actual pressure values ​​and the average value of the multiple second actual pressure values. The predetermined condition also includes: the change amplitude of the multiple third measured pressure values ​​does not exceed 10% of the average value of the first actual pressure value, and the change amplitude of the multiple fourth measured pressure values ​​does not exceed 10% of the average value of the second actual pressure value.

[0010] In some possible implementations, the fluid system further includes a valve assembly, the valve assembly is used to be installed on the pipeline consumables, and the valve assembly includes a plurality of switch valves. The airtightness detection method further includes: selectively opening the switch valve located on the first side so that the pipeline consumables connected to the first side are connected to the first side; selectively opening the switch valve located on the second side so that the pipeline consumables connected to the second side are connected to the second side, and the pipeline consumables connected to the first side are disconnected from the pipeline consumables connected to the second side.

[0011] The second aspect of the present application provides a fluid system, including pipeline consumables, wherein the pipeline consumables include a working pipeline. The fluid system also includes a power piece, a first pressure sensor, a second pressure sensor, and a processor, wherein the power piece, the first pressure sensor, and the second pressure sensor are used to be installed on the working pipeline, and the first pressure sensor and the second pressure sensor are respectively located on the first side and the second side of the power piece. The processor is used to: open the power piece so that the gas in the working pipeline flows from the first side to the second side; close the power piece, and within a first time period after closing the power piece, obtain the first actual pressure value in the working pipeline located on the first side measured by the first pressure sensor, and obtain the second actual pressure value in the working pipeline located on the second side measured by the second pressure sensor; combine the first actual pressure value and the second actual pressure value to initially judge the air tightness of the pipeline consumables.

[0012] In some possible implementations, the processor is also used to: reopen the power component so that the gas in the working pipeline flows in the direction from the second side to the first side; close the power component, and within a first time period after closing the power component, obtain a third actual pressure value in the working pipeline located on the first side measured by the first pressure sensor, and obtain a fourth actual pressure value in the working pipeline located on the second side measured by the second pressure sensor; and secondarily judge the air tightness of the pipeline consumables by combining the third actual pressure value and the fourth actual pressure value.

[0013] In some possible implementations, the fluid system further includes a valve assembly, the valve assembly is used to be installed on the pipeline consumables, and the valve assembly includes a plurality of switch valves. The processor is also used to: selectively open the switch valve located on the first side to connect the pipeline consumables connected to the first side with the first side; selectively open the switch valve located on the second side to connect the pipeline consumables connected to the second side with the second side, and disconnect the pipeline consumables connected to the first side from the pipeline consumables connected to the second side.

[0014] In some possible implementations, the fluid system further includes a display, and the display is used to display installation information of the valve assembly, the first pressure sensor, and the second pressure sensor.

[0015] The present application can automatically detect whether the air tightness of pipeline consumables is qualified through the cooperation of power parts, valve components, first pressure sensors and second pressure sensors, avoid leakage of pipeline consumables, and avoid contamination of instruments and the environment by samples. Moreover, the present application does not need to intervene in the mechanical structure of pipeline consumables when detecting the air tightness of pipeline consumables, which is conducive to ensuring the airtightness and sterility of pipeline consumables. The present application does not need to inject additional liquid when detecting the air tightness of pipeline consumables, but directly promotes the flow of gas in pipeline consumables to change the pressure in the working pipeline, and judges the air tightness of pipeline consumables in combination with the pressure value. Since the compressibility of gas is higher than that of liquid, the pressure change range caused by gas flow in pipeline consumables is larger, which is conducive to improving the detection accuracy. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 A schematic diagram of the structure of a cell sorting system provided in one embodiment of the present application.

[0017] Figure 2 for Figure 1 The cell sorting system shown is a schematic structural diagram after the valve assembly, the power component, the first pressure sensor and the second pressure sensor are installed.

[0018] Figure 3 In some embodiments, Figure 1 Flow chart of the method for detecting airtightness of a cell sorting system is shown.

[0019] Figure 4 Flow chart of airtightness detection method in other embodiments.

[0020] The following specific implementation methods will further illustrate the present application in conjunction with the above-mentioned drawings. DETAILED DESCRIPTION

[0021] In order to more clearly understand the above-mentioned purpose, features and advantages of the present application, the present application is described in detail below in conjunction with the accompanying drawings and specific embodiments. It should be noted that, in the case of no conflict, the embodiments of the present application and the features in the embodiments can be combined with each other. In the following description, many specific details are set forth to facilitate a full understanding of the present application, and the described embodiments are only a part of the embodiments of the present application, rather than all of the embodiments.

[0022] It should be noted that, although a logical order is shown in the flowchart, in some cases, the steps shown or described may be performed in an order different from that in the flowchart. The method disclosed in the embodiment of the present application includes one or more steps or actions for implementing the method. The method steps and / or actions may be interchangeable with each other without departing from the scope of the claims. Unless a specific order of steps or actions is specified, the order and / or use of specific steps and / or actions may be modified without departing from the scope of the claims.

[0023] The present application will be described below with reference to the accompanying drawings and in conjunction with embodiments.

[0024] An embodiment of the present application provides an airtightness detection method, which can be used to detect whether the airtightness of pipeline consumables in a fluid system is qualified. Figure 1 and Figure 2 In some embodiments, the fluid system is a cell sorting system 200, that is, the airtightness detection method can be applied to the cell sorting system 200, and the airtightness of the consumables 20 of the cell sorting system 200 can be detected before cell sorting. Among them, the cell sorting system 200 can be used for blood cell sorting, cell amplification, enrichment, packaging, component separation of other multi-component liquids, or preparation and enrichment of nano-immunomagnetic beads. In other embodiments, the fluid system can also be used in other fields such as automotive parts, home appliances, food, and chemicals.

[0025] The present application is described below by taking the application of the airtightness detection method to the cell sorting system 200 as an example. Figure 1As shown, the cell sorting system 200 further includes a fluid loading mechanism 21, a sample holding member 22, and a component collecting mechanism 23. The fluid loading mechanism 21, the sample holding member 22, and the component collecting mechanism 23 are all connected to the consumable tube 20. The fluid loading mechanism 21 is used to load samples or other fluid components into the consumable tube 20.

[0026] In some embodiments, the fluid loading mechanism 21 includes a sample source loader 21a for holding the sample. The sample source loader 21a can be connected to the sample holding member 22, so that the sample is input into the sample holding member 22. The cell sorting system 200 may also include a centrifugal mechanism (not shown). The centrifugal mechanism is a mechanism with centrifugal function and the function of squeezing the sample holding member 22. The squeezing can be achieved through a mechanical structure, or the squeezing effect can be produced by a change in fluid volume. The centrifugal mechanism is equipped with a rotating connector 24 that is respectively connected to the sample holding member 22 and the pipeline consumable 20. The rotating connector 24 is used to connect the sample holding member 22 and the pipeline consumable 20, so that the centrifugal mechanism drives the sample in the sample holding member 22 to achieve centrifugal stratification and extrude the corresponding layer components into the pipeline consumable 20. Among them, the rotating connector 24 can be a rotary joint.

[0027] In some embodiments, the component collection mechanism 23 includes a waste liquid holding part 23a and a non-waste liquid holding part 23b, and the waste liquid holding part 23a and the non-waste liquid holding part 23b are respectively connected to the pipeline consumables 20. The sample holding part 22 can be connected to the waste liquid holding part 23a, so that the waste liquid separated from the sample holding part 22 is input into the waste liquid holding part 23a. Among them, the waste liquid can be the supernatant after the sample is centrifuged and layered. The sample holding part 22 can also be connected to the non-waste liquid holding part 23b, so that the non-waste liquid components separated by the sample holding part 22 are input into the non-waste liquid holding part 23b. Among them, the non-waste liquid components can be the part other than the supernatant after the sample is centrifuged. In some embodiments, the non-waste liquid holding part 23b includes a destination holding part 23b1 and a non-destination holding part 23b2 respectively connected to the pipeline consumables 20. The target holding part 23 b 1 is used for holding the target liquid among the components separated by the sample holding part 22 , and the non-target holding part 23 b 2 is used for holding the non-target liquid among the components separated by the sample holding part 22 .

[0028] In some embodiments, the cell sorting system 200 further includes a sorting column 25. The fluid loading mechanism 21 further includes a magnetic bead loading component 21b, a binding solution loading component 21c, and an eluent loading component 21d. The magnetic bead loading component 21b, the binding solution loading component 21c, and the eluent loading component 21d are respectively connected to the pipeline consumables 20. The binding solution loading component 21c can be connected to the sample holding component 22, so that the binding solution in the binding solution loading component 21c is input into the sample holding component 22, and the centrifugal mechanism further drives the sample holding component 22 to perform centrifugal movement, so that the binding solution in the sample holding component 22 and the sample are mixed evenly and the target cells are marked. The magnetic bead loading component 21b can be connected to the sample holding component 22, so that the magnetic bead solution in the magnetic bead loading component 21b is input into the sample holding component 22, and the centrifugal mechanism further drives the sample holding component 22 to perform centrifugal movement, so that the magnetic bead solution in the sample holding component 22 and the sample are mixed evenly and combined with the marked target cells. The sorting column 25 can be connected to the sample holding part 22, and the centrifugal mechanism further squeezes the sample holding part 22 to input the sample mixed with the binding liquid and the magnetic beads into the sorting column 25. The sorting column 25 in the magnetic field state can magnetically adsorb the magnetic beads and discharge other components in the sample into the non-target holding part 23b2. The sorting column 25 can be connected to the eluent loading part 21d, so that the eluent in the eluent loading part 21d is injected into the sorting column 25 out of the magnetic field state to elute the target cells into the target holding part 23b1. In some embodiments, each holding part and loading part can be a bag-shaped plastic product.

[0029] In some embodiments, the cell sorting system 200 further includes a pre-sorting column 26, which is connected to the pipeline consumable 20. The pre-sorting column 26 can be connected to the sample holding part 22 and the sorting column 25 respectively, so that the sample after removing the waste liquid in the sample holding part 22 (at this time, the sample in the sample holding part 22 contains target cells, non-target cells, magnetic beads, and binding liquid, etc.) flows through the pre-sorting column 26 and then enters the sorting column 25, thereby filtering the sample entering the sorting column 25.

[0030] In some embodiments, the pipeline consumables 20 include a first main pipeline 20a, a second main pipeline 20b, a third main pipeline 20c, a working pipeline 20d, a loading pipeline 20e, a waste liquid pipeline 20f, a distribution pipeline 20g, a destination pipeline 20h and a non-destination pipeline 20i.

[0031] The first main pipeline 20a can be connected by at least one pipeline through a joint (the figure shows that the first main pipeline 20a is composed of four sections of pipelines). One end of the first main pipeline 20a is connected to the sample holding member 22 through a rotating connector 24. The second main pipeline 20b can be connected by at least one pipeline through a joint (the figure shows that the second main pipeline 20b is composed of two sections of pipelines). One end of the second main pipeline 20b is connected to the first position of the first main pipeline 20a, and the other end of the second main pipeline 20b is connected to the waste liquid pipeline 20f. One end of the working pipeline 20d is connected to the first position of the second main pipeline 20b, and the other end of the working pipeline 20d is divided into a first connecting pipeline 20d1, a second connecting pipeline 20d2 and a third connecting pipeline 20d3, the first connecting pipeline 20d1 is connected to the other end of the first main pipeline 20a, the second connecting pipeline 20d2 is connected to one end of the third main pipeline 20c, and the third connecting pipeline 20d3 is connected to the waste liquid pipeline 20f. The sorting column 25 is connected to the third main pipeline 20c, and the other end of the third main pipeline 20c is connected to the first position of the distribution pipeline 20g. One end of the distribution pipeline 20g is connected to the waste liquid pipeline 20f, and the other end of the distribution pipeline 20g is connected to the destination pipeline 20h. The non-destination pipeline 20i is connected to the second position of the distribution pipeline 20g. One end of the loading pipeline 20e is connected to the second position of the first main pipeline 20a, and the other end of the loading pipeline 20e is connected to the fluid loading mechanism 21.

[0032] like Figure 2As shown, the cell sorting system 200 also includes a valve assembly 10, a first pressure sensor 30, a second pressure sensor 40, a power piece 50 and a processor (not shown). The valve assembly 10, the first pressure sensor 30 and the second pressure sensor 40 are all installed on the pipeline consumable 20. The processor is electrically connected to the valve assembly 10, the first pressure sensor 30, the second pressure sensor 40 and the power piece 50, and the processor is used to control the corresponding operations of the valve assembly 10, the first pressure sensor 30, the second pressure sensor 40 and the power piece 50, and complete the air tightness detection of the pipeline consumable 20 in combination with the automated program. In some embodiments, the cell sorting system 200 also includes a display 60. The display 60 is used to display the operation method of the valve assembly 10, the first pressure sensor 30 and the second pressure sensor 40, such as the display 60 can display relevant text or picture information to remind the user how to operate the valve assembly 10, the first pressure sensor 30, the second pressure sensor 40 and other components during the air tightness detection. The processor may be a central processing unit (CPU), other general-purpose processors, digital signal processors (DSP), application-specific integrated circuits (ASIC), field-programmable gate arrays (FPGA) or other programmable logic devices, discrete gate or transistor logic devices, discrete hardware components, etc. A general-purpose processor may be a microprocessor or any conventional processor, etc.

[0033] After completing the air tightness test, in the subsequent cell sorting process, the valve assembly 10 can also control the fluid loading mechanism 21 to communicate with the sample holding member 22, or control the sample holding member 22 to communicate with the component collection mechanism 23, so as to control the sample or each component entering the pipeline consumable 20 to flow according to the preset timing and preset path. Among them, the valve assembly 10 can include a switch valve installed at each position of the pipeline consumable 20 and used to control the on-off, and the switching of different paths is realized by opening and closing each switch valve. The preset timing and preset path are specifically set according to the type of sample to be sorted. For ease of installation, each switch valve can be clamped at different positions of the pipeline consumable 20, that is, the switch valve can be a clamp valve (such as an electric clamp valve). In the subsequent cell sorting process, the power piece 50 can also provide power to each component or sample flowing through the pipeline consumable 20. The power piece 50 can be a peristaltic pump, etc. For ease of installation, the peristaltic pump can be clamped on the pipeline consumable 20.

[0034] Among them, the valve assembly 10 includes a first switch valve 11, a second switch valve 12, a third switch valve 13, a fourth switch valve 14, a fifth switch valve 15, a sixth switch valve 16, a seventh switch valve 17, an eighth switch valve 18, a ninth switch valve 19, a tenth switch valve 110 and an eleventh switch valve 111.

[0035] The first switch valve 11, the second switch valve 12 and the third switch valve 13 are all used to be installed on the first main pipeline 20a, and the first switch valve 11 is located between the rotating connector 24 and the second main pipeline 20b, the second switch valve 12 is located between the second main pipeline 20b and the loading pipeline 20e, and the third switch valve 13 is located between the loading pipeline 20e and the first connecting pipeline 20d1. The fourth switch valve 14 and the fifth switch valve 15 are all used to be installed on the second main pipeline 20b, and the fourth switch valve 14 is located between the first main pipeline 20a and the working pipeline 20d, and the fifth switch valve 15 is located between the working pipeline 20d and the waste liquid pipeline 20f. The sixth switch valve 16 is used to be installed on the third main pipeline 20c, and the sixth switch valve 16 is located between the second connecting pipeline 20d2 and the sorting column 25. The seventh switch valve 17 and the eighth switch valve 18 are both used to be installed on the waste liquid pipeline 20f, and the seventh switch valve 17 is located between the third connecting pipeline 20d3 and the second main pipeline 20b, and the eighth switch valve 18 is located between the distribution pipeline 20g and the end of the waste liquid pipeline 20f away from the distribution pipeline 20g. The ninth switch valve 19 and the tenth switch valve 110 are both used to be installed on the distribution pipeline 20g, and the ninth switch valve 19 is located between the third main pipeline 20c and the non-destination pipeline 20i, and the tenth switch valve 110 is located between the third main pipeline 20c and the destination pipeline 20h. The eleventh switch valve 111 is used to be installed on the non-destination pipeline 20i. Of course, each fluid loading mechanism 21 is also provided with a switch valve (not shown in the figure), and by operating the switch valve, the fluid in the fluid loading mechanism 21 can be output to the outside.

[0036] In the subsequent cell sorting process, when it is necessary to output the waste liquid in the sample holding part 22 to the waste liquid holding part 23a, the first switch valve 11, the fourth switch valve 14, the fifth switch valve 15 and the eighth switch valve 18 are opened, and the other switch valves are closed. When it is necessary to load the sample, magnetic bead solution, binding solution or eluent in the fluid loading mechanism 21 into the sample holding part 22, the switch valve (not shown in the figure), the third switch valve 13, the fourth switch valve 14 and the first switch valve 11 set on the loading fluid loading mechanism 21 are opened, the other switch valves are closed, and the power part 50 starts to work. When it is necessary to output non-target cells to the non-target holding part 23b2, the first switch valve 11, the fourth switch valve 14, the fifth switch valve 15, the seventh switch valve 17, the sixth switch valve 16, the ninth switch valve 19 and the eleventh switch valve 111 are opened, and the other switch valves are closed. When the target cells need to be output to the target containing part 23b1, the first switch valve 11, the fourth switch valve 14, the fifth switch valve 15, the seventh switch valve 17, the sixth switch valve 16 and the tenth switch valve 110 are opened, and the other switch valves are closed.

[0037] like Figure 1 As shown, in some embodiments, the pipeline consumable 20 also includes a pre-sorting pipeline 20j, which is connected to the connecting point of the first main pipeline 20a and the first connecting pipeline 20d1. The pre-sorting column 26 is used to be installed on the pre-sorting pipeline 20j. Correspondingly, the valve assembly 10 also includes a twelfth switch valve 112 and a thirteenth switch valve 113. The twelfth switch valve 112 is used to be installed on the first main pipeline 20a. The thirteenth switch valve 113 is used to be installed on the pre-sorting pipeline 20j and is located between the first main pipeline 20a and the sorting column 25. In the subsequent cell sorting process, when it is necessary to output the sample after removing the waste liquid in the sample holding part 22 to the pre-sorting column 26, the first switch valve 11, the second switch valve 12, the third switch valve 13, the twelfth switch valve 112 and the thirteenth switch valve 113 are opened, and the other switch valves are closed.

[0038] The power member 50, the first pressure sensor 30 and the second pressure sensor 40 are all installed on the working pipeline 20d. The first pressure sensor 30 is located on the first side 50a of the power member 50, and the second pressure sensor 40 is located on the second side 50b of the power member 50. The first pressure sensor 30 and the second pressure sensor 40 are respectively used to measure the pressure values ​​in the working pipeline 20d on both sides of the power member 50. Figure 3 The specific functions of the power component 50, the first pressure sensor 30 and the second pressure sensor 40 are described.

[0039] See also Figure 3, is a flow chart of the air tightness detection method for the cell sorting system 200 provided in the present application. According to different requirements, the order of the steps of the above method can be changed, and some steps can be omitted or combined. The above air tightness detection method includes the following steps:

[0040] Step S1, providing the above-mentioned cell sorting system 200, which includes a pipeline consumable 20 and a valve assembly 10, a first pressure sensor 30, a second pressure sensor 40 and a power piece 50 respectively installed on the pipeline consumable 20. Among them, the first pressure sensor 30, the second pressure sensor 40 and the power piece 50 are all installed on the working pipeline 20d of the pipeline consumable 20, and the first pressure sensor 30 and the second pressure sensor 40 are respectively located on the first side 50a and the second side 50b of the power piece 50.

[0041] The switch valve on the loading pipeline 20 e may be closed, thereby closing the inlet of the fluid loading mechanism 21 to prevent the sample or other components in the fluid loading mechanism 21 from flowing out.

[0042] Step S2, turning on the power member 50 on the working pipeline 20d, so that the gas (eg, air) in the working pipeline 20d flows in a direction from the first side 50a to the second side 50b.

[0043] In some embodiments, the power member 50 is a peristaltic pump. The peristaltic pump can operate clockwise and squeeze the working pipeline 20d in the direction from the first side 50a to the second side 50b, so that the gas in the working pipeline 20d flows in the direction from the first side 50a to the second side 50b. Since the gas flows in the direction from the first side 50a to the second side 50b, the air in the working pipeline 20d located on the first side 50a of the power member 50 is reduced, and therefore the pressure value in the working pipeline 20d located on the first side 50a of the power member 50 is reduced (e.g., expressed as negative pressure). Similarly, the air in the working pipeline 20d located on the second side 50b of the power member 50 increases, and therefore, the pressure value in the working pipeline 20d located on the second side 50b of the power member 50 increases (e.g., expressed as positive pressure).

[0044] In some embodiments, before the power member 50 is turned on, the switch valve located on the first side 50a of the power member 50 and the switch valve located on the second side 50b of the power member 50 can be selectively opened first. The switch valve located on the first side 50a of the power member 50 is selectively opened for the purpose of connecting the pipeline consumables 20 connected to the first side 50a of the power member 50 with the first side 50a of the power member 50. Similarly, the switch valve located on the second side 50b of the power member 50 is selectively opened for the purpose of connecting the pipeline consumables 20 connected to the second side 50b of the power member 50 with the second side 50b of the power member 50. Therefore, it is convenient to detect the air tightness of each path of the pipeline consumables 20 later. At the same time, after the switch valve is selectively opened, it is necessary to meet the following conditions: the pipeline consumables 20 connected to the first side 50a of the power member 50 are disconnected from the pipeline consumables 20 connected to the second side 50b of the power member 50. In this way, when the gas in the working pipeline 20d flows in the direction from the first side 50a to the second side 50b, so that the working pipeline 20d located on the first side 50a of the power component 50 presents a negative pressure and the working pipeline 20d located on the second side 50b of the power component 50 presents a positive pressure, the pipeline consumable 20 connected to the first side 50a of the power component 50 presents a negative pressure, and the pipeline consumable 20 connected to the second side 50b of the power component 50 presents a positive pressure, and the negative pressure in the pipeline consumable 20 connected to the first side 50a of the power component 50 and the positive pressure in the pipeline consumable 20 connected to the second side 50b of the power component 50 will not affect each other.

[0045] For example, in Figure 1 In the illustrated embodiment, the first switch valve 11 and the fourth switch valve 14 located on the first side 50a of the power member 50 can be selectively opened. At the same time, the third switch valve 13, the sixth switch valve 16, the seventh switch valve 17, the ninth switch valve 19, the twelfth switch valve 112 and the thirteenth switch valve 113 located on the second side 50b of the power member 50 can be selectively opened. At this time, all the pipeline consumables 20 connected to the first side 50a of the power member 50 can present negative pressure, and all the pipeline consumables 20 connected to the first side 50a of the power member 50 can present positive pressure. At the same time, it can also prevent the pipeline consumables 20 presenting negative pressure from being connected to the pipeline consumables 20 presenting positive pressure.

[0046] Step S3, turn off the power component 50. Within the first time period after turning off the power component 50, measure the actual pressure value in the working pipeline 20d on the first side 50a of the power component 50 through the first pressure sensor 30, which is recorded as the first actual pressure value P1, and measure the actual pressure value in the working pipeline 20d on the second side 50b of the power component 50 through the second pressure sensor 40, which is recorded as the second actual pressure value P2.

[0047] In some embodiments, before the switch valve is selectively opened, the empty pipe pressure value in the working pipe 20d of the first side 50a of the power member 50 is first measured by the first pressure sensor 30, which is recorded as the first empty pipe pressure value P 01 The empty pipe pressure value in the working pipeline 20d on the second side 50b of the power member 50 is measured by the second pressure sensor 40, which is recorded as the second empty pipe pressure value P 02 Specifically, after the first pressure sensor 30 and the second pressure sensor 40 are installed on the working pipeline 20d, the pressure value in the working pipeline 20d can be stabilized after standing for a period of time. Then, the first pressure sensor 30 and the second pressure sensor 40 respectively measure the empty pipe pressure values ​​in the working pipeline 20d on both sides of the power component 50. In order to improve the measurement accuracy, five empty pipe pressure values ​​in the working pipeline 20d on the first side 50a of the power component 50 can be read within 1 second, and then the average value of the five empty pipe pressure values ​​is calculated, and the average value is the first empty pipe pressure value P 01 Similarly, five empty pipe pressure values ​​in the working pipe 20d of the second side 50b of the power member 50 can be read within 1 second, and then the average value of the five empty pipe pressure values ​​is calculated, and the average value is the second empty pipe pressure value P 02 .

[0048] Then, within a first time period after the power member 50 is turned off, a plurality of pressure values ​​in the working pipeline 20d on the first side 50a of the power member 50 are continuously measured by the first pressure sensor 30, which are recorded as the first measured pressure value P 11 The second pressure sensor 40 continuously measures multiple pressure values ​​in the working pipeline 20d on the second side 50b of the power member 50, which are recorded as the second measured pressure value P 12 For example, in some embodiments, the first time period is within 2 seconds after the power member 50 is turned off. During the 2 seconds, the pressure value in the working pipeline 20d of the first side 50a of the power member 50 is read every 200 milliseconds, and a total of 10 first measured pressure values ​​P are obtained. 11 At the same time, the pressure value in the working pipeline 20d of the second side 50b of the power member 50 is read every 200 milliseconds, and a total of 10 second measured pressure values ​​P are obtained. 12 .

[0049] Then, according to the first empty pipe pressure value P 01 and each first measured pressure value P 11 Calculate the first actual pressure value P1 in the working pipeline 20d on the first side 50a of the power member 50, and calculate the first actual pressure value P1 in the working pipeline 20d on the first side 50a of the power member 50 according to the second empty pipe pressure value P 02 and each second measured pressure value P 12 The second actual pressure value P2 in the working pipeline 20d on the second side 50b of the power member 50 is calculated. The first actual pressure value P1 = P 11-P 01 , the second actual pressure value P2 = P 12 -P 02 . It can be understood that at this time, 10 first actual pressure values ​​P1 and 10 second actual pressure values ​​P2 are obtained. In some embodiments of the present application, the pipeline consumables 20 including the working pipeline 20d are hoses. When the first pressure sensor 30 is installed on the working pipeline 20d, the working pipeline 20d will produce elastic deformation and apply pressure to the gas in the working pipeline 20d. By calculating the difference between the first measured pressure value and the first empty pipe pressure value, the effect of the elastic deformation of the working pipeline 20d after the first pressure sensor 30 is installed and the effect on the gas in the working pipeline 20d can be offset, thereby facilitating the acquisition of the true pressure in the working pipeline 20d on the first side 50a of the power component 50. Similarly, by calculating the difference between the second measured pressure value and the second empty pipe pressure value, it is beneficial to obtain the true pressure in the working pipeline 20d on the second side 50b of the power component 50.

[0050] Step S4 , combining the first actual pressure value P1 and the second actual pressure value P2 to initially determine whether the air tightness of the pipeline consumable 20 is qualified.

[0051] In some embodiments, when the first actual pressure value P1 and the second actual pressure value P2 simultaneously meet the following two conditions, it is initially determined that the air tightness of the pipeline consumable 20 is qualified. The two conditions are:

[0052] Condition 1: Each first actual pressure value P1 is less than the first empty pipe pressure value P 01 , each second actual pressure value P2 is greater than the second empty pipe pressure value P 02 Meeting this condition means that the pipeline consumables 20 on both sides of the power member 50 are airtight, so that negative pressure is generated in the working pipeline 20d on the first side 50a of the power member 50 due to gas flow, and positive pressure is generated in the working pipeline 20d on the second side 50b of the power member 50 due to gas flow.

[0053] Condition 2: The range of the absolute values ​​of the first actual pressure values ​​P1 does not exceed 10% of the maximum value among the absolute values ​​of the first actual pressure values ​​P1, and the range of the absolute values ​​of the second actual pressure values ​​P2 does not exceed 10% of the maximum value among the absolute values ​​of the second actual pressure values ​​P2. Meeting this condition means that the pipeline consumables 20 located on both sides of the power component 50 are airtight, so that the working pipeline 20d located on each side of the working pipeline 20d will not cause a sharp change in pressure value due to gas leakage in the first time period.

[0054] It can be understood that the above two conditions can be used to determine the air tightness of the pipeline consumables 20 in the first time period after the power component 50 is turned off, thereby being used to evaluate the air tightness of the pipeline consumables 20 .

[0055] Furthermore, in some embodiments, the air tightness of the pipeline consumables 20 in a second time period after the first time period can be further determined, thereby improving the accuracy of the air tightness determination. That is, when it is determined that the pipeline consumables 20 simultaneously meet the above two conditions, it is further determined whether the pipeline consumables 20 meet the following condition three. Only when condition three is met, the air tightness of the pipeline consumables 20 is determined to be qualified. Specifically, in the second time period after the first time period, the first pressure sensor 30 continuously measures multiple pressure values ​​in the working pipeline 20d of the first side 50a of the power component 50, which are recorded as the third measured pressure value P 21 , the second pressure sensor 40 continuously measures a plurality of pressure values ​​in the working pipeline 20d on the second side 50b of the power member 50, which are recorded as the fourth measured pressure value P 22 For example, in some embodiments, the second time period is from the 3rd second to the 60th second after the power member 50 is turned off. During the 58 seconds, a plurality of third measured pressure values ​​P in the working pipeline 20d of the first side 50a of the power member 50 are read every 200 milliseconds. 21 At the same time, a plurality of fourth measured pressure values ​​P in the working pipeline 20d of the second side 50b of the power member 50 are read every 200 milliseconds. 22 Then, the average value X1 of the plurality of first actual pressure values ​​P1 and the average value X2 of the plurality of second actual pressure values ​​P2 obtained in step S4 are calculated, and then the judgment of condition three is performed.

[0056] Condition 3: Multiple consecutive third measured pressure values ​​P 21 The change range does not exceed 10% of the average value X1, and the continuous fourth measured pressure values ​​P 22 The change amplitude of does not exceed 10% of the average value X2. Meeting this condition means that the air tightness of the pipeline consumables 20 located on both sides of the power component 50 is still good in the second time period, so that the working pipeline 20d located on each side of the working pipeline 20d will not cause a sharp change in pressure value due to gas leakage in the second time period. In some embodiments, the three third measured pressure values ​​P measured continuously can be judged 21 Whether the change range exceeds 10% of the average value X1, the three fourth measured pressure values ​​P 22 Whether the change in exceeds 10% of the mean value X2.

[0057] It can be understood that after step S4, the air tightness of the pipeline consumables 20 can be preliminarily judged (hereinafter referred to as: initial air tightness test). After the initial air tightness test, what is actually measured is the air tightness of the pipeline consumables 20 located on the first side 50a of the power component 50 when a negative pressure state is present and the pipeline consumables 20 located on the second side 50b of the power component 50 when a positive pressure state is present. In some embodiments, after the initial air tightness test fails, it is judged that the sealing of the pipeline consumables 20 does not meet the requirements and the test fails. On the contrary, after the initial air tightness test is qualified, a secondary air tightness test can be further performed, that is, the air tightness of the pipeline consumables 20 located on the first side 50a of the power component 50 when a positive pressure state is present and the pipeline consumables 20 located on the second side 50b of the power component 50 when a negative pressure state is present is measured, thereby improving the accuracy of the air tightness judgment. That is, after the initial airtightness test in step S4 is qualified, the airtightness test of the present application may further include the following steps:

[0058] Step S5, reopening the power member 50, so that the gas (eg, air) in the working pipeline 20d flows in a direction from the second side 50b to the first side 50a.

[0059] In some embodiments, when the power member 50 is a peristaltic pump, the peristaltic pump can run counterclockwise and squeeze the working pipeline 20d in the direction from the second side 50b to the first side 50a, so that the gas in the working pipeline 20d flows in the direction from the second side 50b to the first side 50a. Since the gas flows in the direction from the second side 50b to the first side 50a, the air in the working pipeline 20d located on the first side 50a of the power member 50 increases, and thus the pressure value in the working pipeline 20d located on the first side 50a of the power member 50 increases (e.g., manifested as positive pressure). Similarly, the air in the working pipeline 20d located on the second side 50b of the power member 50 decreases, and therefore the pressure value in the working pipeline 20d located on the second side 50b of the power member 50 decreases (e.g., manifested as negative pressure).

[0060] In some embodiments, the switch valve opened in step S2 remains open in step S5, so that the consumable pipe 20 connected to the first side 50a of the power member 50 is connected to the first side 50a of the power member 50, the consumable pipe 20 connected to the second side 50b of the power member 50 is connected to the second side 50b of the power member 50, and the consumable pipe 20 connected to the first side 50a of the power member 50 is disconnected from the consumable pipe 20 connected to the second side 50b of the power member 50. In other embodiments, the switch valve opened in step S2 is at least partially closed in step S5. For example, in the case of Figure 1In the illustrated embodiment, the first switch valve 11 is closed in step S5, thereby preventing the sample holder 22 (in some embodiments, a bag-shaped plastic product) from expanding in volume under positive pressure, making it impossible to form a suitable positive pressure in the first main line 20a.

[0061] Step S6, turn off the power component 50. Within the first time period after turning off the power component 50, measure the actual pressure value in the working pipeline 20d on the first side 50a of the power component 50 through the first pressure sensor 30, which is recorded as the third actual pressure value P3, and measure the actual pressure value in the working pipeline 20d on the second side 50b of the power component 50 through the second pressure sensor 40, which is recorded as the fourth actual pressure value P4.

[0062] In which, step S6 may adopt a method similar to that in step S3 to determine the third actual pressure value P3 and the fourth actual pressure value P4, which will not be described in detail.

[0063] Step S7 , secondarily judging the air tightness of the pipeline consumable 20 in combination with the third actual pressure value P3 and the fourth actual pressure value P4 .

[0064] Among them, step S7 can use a method similar to step S4 to determine the airtightness of the pipeline consumables 20, which is not repeated here. After the secondary airtightness test fails, it is judged that the sealing of the pipeline consumables 20 does not meet the requirements and the test fails. Otherwise, it is judged that the sealing of the pipeline consumables 20 meets the requirements and the test passes.

[0065] like Figure 4 As shown, in other embodiments, if the initial airtightness test fails after step S4, in order to avoid misjudgment caused by abnormal fluctuations in the data of the pressure sensor, the initial airtightness test may be performed again (i.e., step S3 and step S4 are performed again). If both initial airtightness tests fail, the airtightness of the pipeline consumable 20 is finally determined to be unqualified.

[0066] Similarly, if the secondary airtightness test fails, in order to avoid misjudgment caused by abnormal fluctuations in the pressure sensor data, the secondary airtightness test can be repeated (i.e., step S6 and step S7 are repeated). If both secondary airtightness tests fail, the airtightness of the pipeline consumable 20 is finally determined to be unqualified.

[0067] The present application can automatically detect whether the air tightness of the pipeline consumable 20 is qualified through the cooperation of the power part 50, the valve assembly 10, the first pressure sensor 30 and the second pressure sensor 40. When the fluid system is a cell sorting system, the pipeline consumable 20 can be prevented from leaking during cell sorting, thereby ensuring the stability of the cell sorting process, and can also prevent the sample from contaminating the instrument and the laboratory environment, avoiding fire hazards and biochemical pollution risks. Moreover, the present application does not need to intervene in the mechanical structure in the pipeline consumable 20 when detecting the air tightness of the pipeline consumable 20, so as to ensure the airtightness and sterility of the pipeline consumable 20. The present application does not need to inject additional liquid when detecting the air tightness of the pipeline consumable 20, but directly promotes the gas flow in the pipeline consumable 20 to change the pressure in the working pipeline 20d, and judges the air tightness of the pipeline consumable 20 in combination with the pressure value. Since the compressibility of the gas is higher than that of the liquid, the pressure change range caused by the gas flow in the pipeline consumable 20 is larger, which is conducive to improving the detection accuracy. In addition, the present application can also avoid the risk of damage and a small amount of leakage of the pipeline consumables 20 after the liquid flow reaches the pressure threshold but cannot be detected in time. Finally, the present application selectively opens the switch valve so that the air tightness of each path in the pipeline consumables 20 can be tested, and the air tightness of the sample holder 22 itself can also be tested.

[0068] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present application and are not intended to limit it. Although the present application has been described in detail with reference to the preferred embodiments, a person of ordinary skill in the art should understand that the technical solution of the present application may be modified or replaced by equivalents without departing from the spirit and scope of the technical solution of the present application.

Claims

1. A method for detecting air tightness, used in a fluid system, characterized in that: The airtightness detection method comprises: Provide the fluid system, the fluid system comprising a pipeline consumable, a power piece, a first pressure sensor and a second pressure sensor, the pipeline consumable comprising a working pipeline, the power piece, the first pressure sensor and the second pressure sensor are installed on the working pipeline, and the first pressure sensor and the second pressure sensor are respectively located on a first side and a second side of the power piece; Turning on the power member to allow the gas in the working pipeline to flow from the first side to the second side; Turning off the power component, and within a first time period after turning off the power component, measuring a first actual pressure value in the working pipeline located on the first side by the first pressure sensor, and measuring a second actual pressure value in the working pipeline located on the second side by the second pressure sensor; The air tightness of the pipeline consumable is initially determined in combination with the first actual pressure value and the second actual pressure value.

2. The airtightness detection method according to claim 1, characterized in that: The airtightness detection method further comprises: Restarting the power member so that the gas in the working pipeline flows from the second side to the first side; Turning off the power component, and within a first time period after turning off the power component, measuring a third actual pressure value in the working pipeline located on the first side by the first pressure sensor, and measuring a fourth actual pressure value in the working pipeline located on the second side by the second pressure sensor; The air tightness of the pipeline consumable is secondarily determined in combination with the third actual pressure value and the fourth actual pressure value.

3. The airtightness detection method according to claim 1, characterized in that: Before starting the power part, the air tightness detection method further includes: Measuring a first empty pipe pressure value in the working pipeline located at the first side by using the first pressure sensor, and measuring a second empty pipe pressure value in the working pipeline located at the second side by using the second pressure sensor; In a first time period after the power component is turned off, a first measured pressure value in the working pipeline located on the first side is measured by the first pressure sensor, and a second measured pressure value in the working pipeline located on the second side is measured by the second pressure sensor; The first actual pressure value is calculated according to the difference between the first measured pressure value and the first empty pipe pressure value, and the second actual pressure value is calculated according to the difference between the second measured pressure value and the second empty pipe pressure value.

4. The airtightness detection method according to claim 3, characterized in that: The first measured pressure value is a plurality of first measured pressure values ​​continuously measured in a first time period after the power member is turned off, the second measured pressure value is a plurality of second measured pressure values ​​continuously measured in a first time period after the power member is turned off, the first actual pressure value is a difference between each of the first measured pressure values ​​and the first empty pipe pressure value, and the second actual pressure value is a difference between each of the second measured pressure values ​​and the second empty pipe pressure value; When the first actual pressure value and the second actual pressure value meet a predetermined condition, it is initially determined that the air tightness of the pipeline consumable is qualified, and the predetermined condition includes: Each of the first actual pressure values ​​is smaller than the first empty pipe pressure value, and each of the second actual pressure values ​​is larger than the second empty pipe pressure value; The range of absolute values ​​of the first actual pressure values ​​does not exceed 10% of the maximum absolute value of the first actual pressure values, and the range of absolute values ​​of the second actual pressure values ​​does not exceed 10% of the maximum absolute value of the second actual pressure values.

5. The airtightness detection method according to claim 4, characterized in that: After the power part is turned off, the air tightness detection method further includes: In a second time period after the first time period, continuously measuring a plurality of third measured pressure values ​​in the working pipeline on the first side by the first pressure sensor, and continuously measuring a plurality of fourth measured pressure values ​​in the working pipeline on the second side by the second pressure sensor; calculating an average value of a plurality of first actual pressure values ​​and an average value of a plurality of second actual pressure values; The predetermined conditions also include: The variation range of the plurality of third measured pressure values ​​does not exceed 10% of the average value of the first actual pressure value, and the variation range of the plurality of fourth measured pressure values ​​does not exceed 10% of the average value of the second actual pressure value.

6. The airtightness detection method according to claim 1, characterized in that: The fluid system further includes a valve assembly, the valve assembly is used to be installed on the pipeline consumables, the valve assembly includes a plurality of switch valves, and the air tightness detection method further includes: Selectively opening the switch valve located at the first side to allow the pipeline consumables connected to the first side to communicate with the first side; The switch valve located on the second side is selectively opened to connect the pipeline consumables connected to the second side to the second side, and the pipeline consumables connected to the first side are disconnected from the pipeline consumables connected to the second side.

7. A fluid system, comprising pipeline consumables, wherein the pipeline consumables include a working pipeline, characterized in that: The fluid system further includes a power piece, a first pressure sensor, a second pressure sensor and a processor, wherein the power piece, the first pressure sensor and the second pressure sensor are used to be installed on the working pipeline, and the first pressure sensor and the second pressure sensor are located on a first side and a second side of the power piece, respectively; The processor is used to: Turning on the power member to allow the gas in the working pipeline to flow from the first side to the second side; Turning off the power component, obtaining a first actual pressure value in the working pipeline on the first side measured by the first pressure sensor within a first time period after turning off the power component, and obtaining a second actual pressure value in the working pipeline on the second side measured by the second pressure sensor; The air tightness of the pipeline consumable is initially determined in combination with the first actual pressure value and the second actual pressure value.

8. The fluid system according to claim 7, characterized in that The processor is further configured to: Restarting the power member so that the gas in the working pipeline flows from the second side to the first side; Turning off the power component, and obtaining a third actual pressure value in the working pipeline located on the first side measured by the first pressure sensor within a first time period after turning off the power component, and obtaining a fourth actual pressure value in the working pipeline located on the second side measured by the second pressure sensor; The air tightness of the pipeline consumable is secondarily determined in combination with the third actual pressure value and the fourth actual pressure value.

9. The fluid system according to claim 7, characterized in that The fluid system further comprises a valve assembly, the valve assembly is used to be installed on the pipeline consumables, and the valve assembly comprises a plurality of switch valves; The processor is further configured to: Selectively opening the switch valve located at the first side to allow the pipeline consumables connected to the first side to communicate with the first side; The switch valve located on the second side is selectively opened to connect the pipeline consumables connected to the second side to the second side, and the pipeline consumables connected to the first side are disconnected from the pipeline consumables connected to the second side.

10. The fluid system according to claim 9, characterized in that The fluid system further includes a display configured to display installation information of the valve assembly, the first pressure sensor, and the second pressure sensor.

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