Artificial breeding method for boschniakia rossica

Abstract

The invention relates to the technical field of boschniakia rossica artificial breeding, and provides a boschniakia rossica artificial breeding method which comprises the following steps: S1, seed treatment: collecting seeds of boschniakia rossica, naturally drying in the shade, and storing in a shading manner, so that the seeds overwinter; s2, preparing the red almonds, simulating the red almonds, and selecting the red almonds with the tree age of more than one year in the boschniakia rossica with the canopy density of 85% or more; s3, variable-temperature germination acceleration: seed germination acceleration is performed before seeding preparation in the current year, seeds are threshed, and a constant-temperature box is adopted for germination acceleration; s4, mixing the soil, and mixing the seeds and the soil according to a ratio of 1: 15 after variable-temperature germination acceleration; s5, preparing root systems, and forming an annular inoculation surface spaced from roots of the alnus japonica on the ground by taking the alnus japonica as a center; s6, sowing: selecting early and middle ten days of May as an inoculation season, directly sowing the seeds mixed with soil in an annular inoculation surface, and then adopting a double-layer covering. By means of the technical scheme, the problems existing in an existing boschniakia rossica artificial inoculation method are solved.

Description

Technical Field

[0001] The invention relates to the technical field of artificial breeding of Cistanche rossica, in particular to an artificial breeding method of Cistanche rossica. Background Art

[0002] The parasitic mechanism of Cistanche deserticola is that the tip of Alnus populus (host) induces the germination of Cistanche deserticola seeds and the tropism of the radicle, and forms a parasitic relationship with Alnus populus (host), forming underground corms, which grow out of the ground after wintering to form Cistanche deserticola plants. Measures such as removing excessively thick ground cover, appropriately cutting roots (forming a large root system) and root system isolation can increase the parasitic rate and artificially cultivate host Alnus populus.

[0003] The disadvantages of the prior art are that artificial inoculation is required at the roots of middle-aged alnus (more than 3 years old) by broadcasting. After inoculation, the plant needs to overwinter, form nodules, and grow tropistically into Cistanche deserticola plants. The operation method is complicated, there are many uncertain factors, the parasitism rate is low, and the yield is affected. The contradiction between supply and demand is still prominent. Cistanche deserticola is inoculated in the alnus (host) habitat, and the growth is disorderly, which is not conducive to scientific observation and research. Summary of the invention

[0004] The technical problem to be solved by the present invention is to provide an artificial breeding method for Cistanche rossica in view of the above-mentioned technical deficiencies, which solves the problems existing in the existing artificial inoculation method for Cistanche rossica.

[0005] The technical solution adopted by the present invention is: to provide a method for artificial breeding of Cistanche rossica, comprising the following steps:

[0006] S1. Seed processing: after collecting the seeds of Cistanche rossica, they are naturally dried in the shade and stored in a dark place to allow the seeds to survive the winter;

[0007] S2. Preparation of Alnus truncatula, Alnus truncatula in bionic environment, Alnus truncatula with a canopy density of 85% or more, select Alnus truncatula with a tree age of more than one year, and cut off the root system of Alnus truncatula in the autumn of the year before seed inoculation;

[0008] S3, variable temperature germination, before sowing in the same year, the seeds are germinated, the seeds are threshed and the temperature is regularly changed between -30 degrees Celsius and +20 degrees Celsius in a constant temperature box, and the germination time is 5-7 days;

[0009] S4. Mix the soil. After changing the temperature to accelerate germination, mix the seeds and soil in a ratio of 1:15, with a water content of 60±5%;

[0010] S5. Root system preparation: With Alnus as the center, a ring-shaped inoculation surface is formed on the ground that is spaced from the roots of Alnus;

[0011] S6. Sowing. The inoculation season is selected in early to mid-May, when the daily average effective accumulated temperature is 10℃. The seeds mixed with soil are sown directly in the ring inoculation surface. The sowing is covered with a mixture of threshed seed shells and soil of Cistanche deserticola capsules to a thickness of 1 cm, and covered with soft humus on the surface to a thickness of 5-10 cm.

[0012] Further optimization of this technical solution also includes the following steps:

[0013] S7. Field management: water the field with atomized spray to maintain the relative humidity of the inoculated surface at 60±5%.

[0014] To further optimize the technical solution, the step S7, field management, further comprises: filling the planting holes after picking with a mixture of threshing shells of Cistanche deserticola capsule seeds and soil in a ratio of 1:15.

[0015] To further optimize the technical solution, in step S1, the overwintering, transportation and storage of the seeds are carried out under conditions of 70% shading.

[0016] To further optimize the technical solution, in step S2, in the autumn of the year before seed inoculation, the root system is cut off at a distance of 40 cm from the root of the alder tree. In step S5, before seed inoculation, the root system is prepared into a circular inoculation surface 20 cm-40 cm away from the root of the alder tree.

[0017] To further optimize the technical solution, step S5 also includes: when inoculating in the current year, removing the covering on the surface of the root system, breaking the root skin, increasing the inoculation surface, and promoting nodule formation.

[0018] To further optimize the technical solution, in step S6, the soft humus on the ground surface is a covering material with a broad-leaved humus content of more than 5% and a pH value between 3-6.

[0019] To further optimize the technical solution, the step S7, field management, also includes: preventing rodent damage.

[0020] The beneficial effects of the present invention are:

[0021] 1. Through early intervention of seeds and the use of variable temperature germination seed modulation technology, seed germination can be promoted, and only one-year-old Alnus root system is needed to meet the inoculation needs.

[0022] 2. Mixing seeds and soil in a ratio of 1:15 will help the seeds maintain moisture content.

[0023] 3. Breaking the root bark for sowing can increase the inoculation surface, promote nodule formation, and increase the parasitic probability of Cistanche deserticola.

[0024] 4. After picking, fill the planting holes with a mixture of seed husks and soil in a ratio of 1:15. This can ensure that the inoculation environment is not destroyed and increase the parasitic rate and growth rate in the following year. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 It is a schematic diagram of the annular inoculation surface of the present invention;

[0026] Explanation of the marks in the figure: 1. Alnus; 2. Annular inoculation surface; DETAILED DESCRIPTION

[0027] The present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0028] In this article, "one" not only means "only one", but also can mean "more than one", and "several" includes "two" and "more than two".

[0029] In this document, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0030] In addition, in the description of the present application, the terms "first", "second", etc. are only used to distinguish the description and cannot be understood as indicating or implying relative importance.

[0031] like Figure 1 As shown, the artificial breeding method of Cistanche rossica comprises the following steps:

[0032] S1. Seed processing: After collecting the seeds of Cistanche deserticola, dry them naturally in the shade and store them in a dark place to allow the seeds to overwinter. By collectively storing the seeds overwintering, the seeds can be better protected.

[0033] S2. Preparation of Alnus, Alnus in bionic environment, Alnus in Cistanche deserticola habitat with a canopy density of 85% or more, select Alnus over one year old, cut off the root system of Alnus in the autumn of the year before seed inoculation; use trees over one year old, the number of hosts is greater. If field inoculation is used, a design of 25% light transmittance is required, and the soil moisture content of the inoculation site is maintained at about 65%.

[0034] S3. Variable temperature germination. Seed germination is carried out before sowing in the same year. The seeds are threshed and the temperature is regularly changed between -30 degrees Celsius and +20 degrees Celsius in a constant temperature box. The germination time is 5-7 days. The seed modulation technology of variable temperature germination is beneficial to improve the development rate and parasite survival rate.

[0035] S4. Mix the soil. After changing the temperature to accelerate germination, mix the seeds and soil in a ratio of 1:15, with a water content of 60±5%;

[0036] S5. Root system preparation: With Alnus as the center, form a ring-shaped inoculation surface separated from the roots of Alnus;

[0037] S6. Sowing. The inoculation season is in early to mid-May, when the daily average effective accumulated temperature is 10℃. The seeds mixed with soil are sown directly in the ring inoculation surface. The seed shells and soil mixture after threshing of Cistanche deserticola capsules are 1cm thick, and the soft humus on the surface is covered with a thickness of 5-10cm. The seeds can parasitize on the cut roots in the ring inoculation surface. The double-layer covering method is more conducive to the growth and survival of plants in the soil environment and season, and the covering can provide rich soil organic matter.

[0038] Further, the method further comprises the following steps:

[0039] S7, field management, watering by atomization spraying, maintaining the relative humidity of the inoculation surface at 60±5%. Step S7, field management also includes: filling the planting holes after picking with a mixture of threshing shells of Cistanche rossica capsules and soil in a ratio of 1:15.

[0040] After sowing, good field management is also required to improve the success rate of parasitic growth. Field management also includes soil maintenance after the harvest of Cistanche deserticola. Filling the planting holes with a mixture of seed husk and soil in a certain proportion helps to ensure that the inoculation environment is not damaged and achieve repeatable artificial planting and breeding.

[0041] Furthermore, in step S1, the overwintering, transportation and storage of the seeds are carried out under conditions of 70% shading.

[0042] The seeds should be protected under 70% light blocking conditions during storage and transportation.

[0043] Further, in step S2, in the autumn of the year before seed inoculation, the root system is cut off at 40 cm from the root of the alder tree. In step S5, before seed inoculation, the root system is prepared as a ring-shaped inoculation surface 20 cm-40 cm away from the root of the alder tree, that is, the ring-shaped inoculation surface is at least 20 cm away from the root of the alder tree and at most 40 cm away, and the ring-shaped inoculation surface is a 20 cm wide circular ring. Step S5 also includes: when inoculating in the current year, removing the covering on the root surface, breaking the root skin, increasing the inoculation surface, and promoting nodule formation.

[0044] The advance treatment of the root system and the use of tools to break the root skin before sowing can increase the inoculation area, promote the growth of nodules, and improve the parasite survival rate. The annular inoculation surface is centered on the root of the alder tree and has a radius of 20cm-40cm from the outer edge of the root of the alder tree.

[0045] Furthermore, in step S6, the soft humus on the ground surface is a covering material having a broad-leaved humus content of more than 5% and a pH value between 3-6.

[0046] Use a double-layer covering method to provide rich soil organic matter, improve survival rate, and promote better plant growth.

[0047] Furthermore, step S7, field management also includes: preventing rodent damage.

[0048] After sowing, prevent rodent infestation that may cause damage to seeds or plants and affect yield.

[0049] It is to be understood that the present invention is described by some embodiments, and it is known to those skilled in the art that various changes or equivalent substitutions may be made to these features and embodiments without departing from the spirit and scope of the present invention. In addition, under the teachings of the present invention, these features and embodiments may be modified to adapt to specific circumstances and materials without departing from the spirit and scope of the present invention. Therefore, the present invention is not limited by the specific embodiments disclosed herein, and all embodiments falling within the scope of the claims of this application are within the scope of protection of the present invention.

Claims

1. A method for artificial breeding of Cistanche rossica, characterized in that: The steps include: S1. Seed processing: after collecting the seeds of Cistanche rossica, they are naturally dried in the shade and stored in a dark place to allow the seeds to survive the winter; S2. Preparation of Alnus truncatula, Alnus truncatula in bionic environment, Alnus truncatula with a canopy density of 85% or more, select Alnus truncatula with a tree age of more than one year, and cut off the root system of Alnus truncatula in the autumn of the year before seed inoculation; S3, variable temperature germination, before sowing in the same year, the seeds are germinated, the seeds are threshed and the temperature is regularly changed between -30 degrees Celsius and +20 degrees Celsius in a constant temperature box, and the germination time is 5-7 days; S4. Mix the soil. After changing the temperature to accelerate germination, mix the seeds and soil in a ratio of 1:15, with a water content of 60±5%; S5. Root system preparation: With Alnus as the center, a ring-shaped inoculation surface is formed on the ground that is spaced from the roots of Alnus; S6. Sowing. The inoculation season is selected in early to mid-May, when the daily average effective accumulated temperature is 10℃. The seeds mixed with soil are sown directly in the ring inoculation surface. The sowing is covered with a mixture of threshed seed shells and soil of Cistanche deserticola capsules to a thickness of 1 cm, and covered with soft humus on the surface to a thickness of 5-10 cm.

2. The artificial breeding method of Cistanche rossica according to claim 1, characterized in that: The following steps are also included: S7. Field management: water the field with atomized spray to maintain the relative humidity of the inoculated surface at 60±5%.

3. The artificial breeding method of Cistanche rossica according to claim 2, characterized in that: The step S7, field management, further comprises: filling the planting holes after picking with a mixture of threshing shells of Cistanche deserticola capsule seeds and soil in a ratio of 1:

15.

4. The artificial breeding method of Cistanche rossica according to claim 1, characterized in that: In step S1, the overwintering, transportation and storage of the seeds are carried out under the condition of 70% shading.

5. The artificial breeding method of Cistanche rossica according to claim 1, characterized in that: In the step S2, in the autumn of the year before the seed inoculation, the root system is cut off at a distance of 40 cm from the root of the alder tree. In the step S5, before the seed inoculation, the root system is prepared into a ring-shaped inoculation surface 20 cm-40 cm away from the root of the alder tree.

6. The artificial breeding method of Cistanche rossica according to claim 5, characterized in that: The step S5 also includes: when inoculating in the current year, removing the covering on the surface of the root system, breaking the root skin, increasing the inoculation surface, and promoting nodule formation.

7. The artificial breeding method of Cistanche rossica according to claim 1, characterized in that: In step S6, the soft humus on the ground surface is a covering material with a broad-leaved humus content of more than 5% and a pH value between 3-6.

8. The artificial breeding method of Cistanche rossica according to claim 2 or 3, characterized in that: The step S7, field management, also includes: preventing rodent damage.

Images