Plant growth salt-resistant agent and preparation method thereof

Abstract

The invention belongs to the field of plant growth regulators, and particularly relates to a plant growth salt-resistant agent and a preparation method thereof.The plant growth salt-resistant agent comprises chitosan oligosaccharide serving as a carrier and brassinolide, naphthylacetic acid and indolebutyric acid which are compounded with the chitosan oligosaccharide, and the plant growth salt-resistant agent can be used for seed soaking, coating, transplanting root soaking or leaf surface spraying of plants. The salt-resistant agent disclosed by the invention can stimulate the potential of plants to resist saline-alkali adversity stress, improve the saline-alkali resistance of the plants and protect the growth safety of the plants in the saline-alkali soil.

Description

Technical Field

[0001] The invention belongs to the field of plant growth regulators, and in particular relates to a plant growth salt-resistance agent and a preparation method thereof. Background Art

[0002] Soil salinization is becoming increasingly serious worldwide, and has become one of the main factors restricting the growth of crops. Saline-alkali land is an important reserve arable land resource, but most crops are non-halophytes. Salt stress will inhibit important processes such as seed germination, growth and development, and flowering and fruiting. Salt stress produces ion toxicity by increasing the ion concentration in plant tissues, and also breaks the balance of reactive oxygen in plants. Excessive accumulation of reactive oxygen can cause cell membrane damage and even cause plant death. Alleviating the damage of salt stress to crops and turning land reserves into arable land increments are of great significance to maintaining food production and increasing farmers' income. When facing a complex and changing environment, plants need to quickly and accurately identify and integrate internal and external signals and then adopt corresponding strategies. The salt tolerance of plants is given by genetic genes, but the salt resistance is induced by external factors. The research and development of salt-resistant agents is of great significance to improving the salt tolerance of existing plants, increasing the yield per unit area of ​​land, improving the production capacity of saline-alkali land, developing and utilizing saline-alkali land, and maintaining food security and ecological security. Summary of the invention

[0003] In order to solve the problem of crops being subjected to salt stress and growth being restricted in the prior art, the present invention mainly provides a salt-resistant agent for promoting plant growth:

[0004] A plant growth salt-resistance agent comprises, by weight, 50-100 parts of chitosan oligosaccharide as a carrier and 20-50 parts of brassinolide, 3-15 parts of naphthylacetic acid and 5-25 parts of indolebutyric acid compounded with the chitosan oligosaccharide.

[0005] Further, water is added to prepare a 30-4000 times dilution.

[0006] A method for preparing the above-mentioned plant growth salt-resistant agent comprises the following steps:

[0007] a. Dissolve chitosan oligosaccharide in 0.5-2% glacial acetic acid solution to obtain chitosan oligosaccharide solution; dissolve naphthaleneacetic acid in hot water to obtain naphthaleneacetic acid solution; then, fully mix with the chitosan oligosaccharide solution and react at room temperature for 12-16 hours; after the reaction is completed, add dimethyl sulfoxide to fully precipitate the product, separate the product, and fully wash and dry it with ethanol to obtain a naphthaleneacetic acid-chitosan oligosaccharide complex;

[0008] b. Place the naphthaleneacetic acid-chitosan oligosaccharide complex in water to obtain a 5-10 mg / mL naphthaleneacetic acid-chitosan oligosaccharide solution; prepare an indoleacetic acid solution; dropwise add the indolebutyric acid solution to the naphthaleneacetic acid-chitosan oligosaccharide solution while stirring, continue stirring for 1-2 hours after the addition is complete, evaporate the solvent, collect the product and fully dry it to obtain a composite precursor;

[0009] c. Mix the composite precursor with brassinolide, moisten with an ethanol aqueous solution, grind to dryness, and obtain a plant growth anti-salt agent after drying.

[0010] Furthermore, the mass ratio of chitosan oligosaccharide to naphthylacetic acid in step a is 5 to 10:1.

[0011] Furthermore, before mixing with the chitosan oligosaccharide solution, the naphthylacetic acid solution is added with 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride and N-hydroxysuccinimide to react for 1 to 2 hours.

[0012] Furthermore, the mass ratio of naphthylacetic acid to 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride is 1:0.8-1.5; the mass ratio of 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride to N-hydroxysuccinimide is 1.5-3:1.

[0013] Furthermore, the step of preparing the indoleacetic acid solution in step b is: dissolving indolebutyric acid in ethanol, and gradually diluting with water to obtain an indolebutyric acid solution.

[0014] Furthermore, the concentration of the indolebutyric acid solution is 1 to 10 g / L.

[0015] Furthermore, the volume ratio of ethanol to water in the ethanol aqueous solution in step c is 1:0.5-2.

[0016] An application of the plant growth salt-resistance agent in plant seed soaking, coating, transplanting root soaking or foliar spraying.

[0017] By adopting the above scheme, the method of the present invention has the following advantages:

[0018] 1. The salt-resistant agent of the present invention can promote the differentiation, elongation and division of cells in the vascular system, has a significant growth-promoting effect on the main roots and fibrous roots of plants, and improves the seed germination rate and the survival rate under adverse stress. It can stimulate the potential of plants to resist saline-alkali stress, enhance the ability of plants to resist salt-alkali stress, and protect the growth safety of plants in saline-alkali land.

[0019] 2. The salt-resistant agent of the present invention has a stimulating effect on the enzyme metabolism system in plants, can enhance photosynthesis, reduce the accumulation of harmful substances in plants due to adverse stress, and induce plants to produce and enhance the ability to resist salt-alkali stress.

[0020] 3. The salt-resistant agent of the present invention is made of several core raw materials including chitosan oligosaccharides, brassinolide, naphthylacetic acid and indolebutyric acid through a specific process. In physical space, chitosan oligosaccharides provide unique grid positions, brassinolide occupies multiple positions, and naphthylacetic acid and indolebutyric acid jointly occupy positions to form an organic complex with complementary functions.

[0021] 4. The present invention compounds several materials, solves the problem of poor water solubility of brassinolide, naphthylacetic acid and indolebutyric acid, improves the water solubility of the product, and prepares it into powder during production. When used, it does not require a special solvent dissolution step, and can be used by simply diluting with water. It is easier to transport, carry and store, and is convenient to use.

[0022] 5. The salt-resistant agent of the present invention has good water solubility, is more easily absorbed by plants, has good affinity with plants, is non-toxic and harmless, and after being diluted with water a certain multiple, is suitable for seed soaking, coating, transplanting root soaking, foliar spraying, etc. of various plants.

[0023] 6. The salt-resistant agent of the present invention has made a breakthrough in applying brassinolide, naphthylacetic acid and indolebutyric acid, which promote plant growth, into the salt-resistant agent, and has discovered the role of several auxins in synergistically improving the resistance of plants to salt-alkali stress. After being diluted to many times, the plants can still grow well in high-salt soil. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 This is a photo of seedlings being raised in trays after the seeds were soaked with the salt-resistant agent of Example 1.

[0025] Figure 2 These are photos comparing plant heights after treatment with the salt-resistant agent of Example 1 and transplanting into saline soil 10 days later.

[0026] Figure 3 These are comparison photos of seedling emergence after direct seeding in saline soil using the salt-resistant agent of Comparative Example 1.

[0027] Figure 4 These are photos comparing the resistance of rice seedlings to long-term high temperature, drought stress, and saline soil. DETAILED DESCRIPTION

[0028] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0029] Example 1: (1) Dissolve 7 g of chitosan oligosaccharide in 1% glacial acetic acid solution to obtain a chitosan oligosaccharide solution; dissolve 1 g of naphthylacetic acid in hot water to obtain a naphthylacetic acid solution; add 1 g of 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride and 0.5 g of N-hydroxysuccinimide to the naphthylacetic acid solution to react for 2 h, then fully mix with the chitosan oligosaccharide solution and react at room temperature for 14 h; after the reaction is completed, add dimethyl sulfoxide to fully precipitate the product, separate the product and wash it three times with anhydrous ethanol, and dry it to obtain a naphthylacetic acid-chitosan oligosaccharide complex;

[0030] (2) placing a naphthaleneacetic acid-chitosan oligosaccharide complex in water to obtain an 8 mg / mL naphthaleneacetic acid-chitosan oligosaccharide solution; dissolving 1.5 g of indolebutyric acid in ethanol, and gradually diluting with water to obtain a 5 g / L indolebutyric acid solution; adding the indolebutyric acid solution dropwise to the naphthaleneacetic acid-chitosan oligosaccharide solution while stirring, and continuing to stir for 2 hours after the addition is complete, evaporating the solvent, collecting the product and fully drying it to obtain a composite precursor;

[0031] (3) The composite precursor is mixed with 3 g of brassinolide, moistened with a 50% ethanol aqueous solution, ground to dryness, and dried to obtain a plant growth salt-resistant agent.

[0032] Example 2: (1) Dissolve 10 g of chitosan oligosaccharide in 1% glacial acetic acid solution to obtain a chitosan oligosaccharide solution; dissolve 1 g of naphthylacetic acid in hot water to obtain a naphthylacetic acid solution; add 1 g of 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride and 0.5 g of N-hydroxysuccinimide to the naphthylacetic acid solution to react for 2 h, then fully mix with the chitosan oligosaccharide solution and react at room temperature for 14 h; after the reaction is completed, add dimethyl sulfoxide to fully precipitate the product, separate the product and wash it three times with anhydrous ethanol, and then dry it to obtain a naphthylacetic acid-chitosan oligosaccharide complex;

[0033] (2) placing a naphthaleneacetic acid-chitosan oligosaccharide complex in water to obtain an 8 mg / mL naphthaleneacetic acid-chitosan oligosaccharide solution; dissolving 1.5 g of indolebutyric acid in ethanol, and gradually diluting with water to obtain a 5 g / L indolebutyric acid solution; adding the indolebutyric acid solution dropwise to the naphthaleneacetic acid-chitosan oligosaccharide solution while stirring, and continuing to stir for 2 hours after the addition is complete, evaporating the solvent, collecting the product and fully drying it to obtain a composite precursor;

[0034] (3) The composite precursor is mixed with 5 g of brassinolide, moistened with a 50% ethanol aqueous solution, ground to dryness, and dried to obtain a plant growth salt-resistant agent.

[0035] Example 3: (1) Dissolve 7 g of chitosan oligosaccharide in 1% glacial acetic acid solution to obtain a chitosan oligosaccharide solution; dissolve 1.5 g of naphthylacetic acid in hot water to obtain a naphthylacetic acid solution; add 1 g of 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride and 0.5 g of N-hydroxysuccinimide to the naphthylacetic acid solution to react for 2 h, then fully mix with the chitosan oligosaccharide solution and react at room temperature for 14 h; after the reaction is completed, add dimethyl sulfoxide to fully precipitate the product, separate the product and wash it three times with anhydrous ethanol, and then dry it to obtain a naphthylacetic acid-chitosan oligosaccharide complex;

[0036] (2) using a naphthaleneacetic acid-chitosan oligosaccharide complex in water to prepare an 8 mg / mL naphthaleneacetic acid-chitosan oligosaccharide solution; dissolving 2.5 g of indolebutyric acid in ethanol, and gradually diluting with water to obtain a 5 g / L indolebutyric acid solution; adding the indolebutyric acid solution dropwise to the naphthaleneacetic acid-chitosan oligosaccharide solution while stirring, and continuing to stir for 2 hours after the addition is complete, evaporating the solvent, collecting the product and fully drying it to obtain a composite precursor;

[0037] (3) The composite precursor is mixed with 3 g of brassinolide, moistened with a 50% ethanol aqueous solution, ground to dryness, and dried to obtain a plant growth salt-resistant agent.

[0038] Example 4: (1) 5 g of chitosan oligosaccharide was dissolved in 1% glacial acetic acid solution to obtain a chitosan oligosaccharide solution; 1 g of naphthylacetic acid was dissolved in hot water to obtain a naphthylacetic acid solution; 1 g of 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride and 0.5 g of N-hydroxysuccinimide were added to the naphthylacetic acid solution to react for 2 h, and then the mixture was fully mixed with the chitosan oligosaccharide solution and reacted at room temperature for 14 h; after the reaction was completed, dimethyl sulfoxide was added to fully precipitate the product, the product was separated and washed three times with anhydrous ethanol, and dried to obtain a naphthylacetic acid-chitosan oligosaccharide complex;

[0039] (2) using a naphthaleneacetic acid-chitosan oligosaccharide complex in water to prepare an 8 mg / mL naphthaleneacetic acid-chitosan oligosaccharide solution; dissolving 2.5 g of indolebutyric acid in ethanol, and gradually diluting with water to obtain a 5 g / L indolebutyric acid solution; adding the indolebutyric acid solution dropwise to the naphthaleneacetic acid-chitosan oligosaccharide solution while stirring, and continuing to stir for 2 hours after the addition is complete, evaporating the solvent, collecting the product and fully drying it to obtain a composite precursor;

[0040] (3) The composite precursor is mixed with 5 g of brassinolide, moistened with a 50% ethanol aqueous solution, ground to dryness, and dried to obtain a plant growth salt-resistant agent.

[0041] Example 5: (1) Dissolve 7 g of chitosan oligosaccharide in 1% glacial acetic acid solution to obtain a chitosan oligosaccharide solution; dissolve 1 g of naphthylacetic acid in hot water to obtain a naphthylacetic acid solution; add 1 g of 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride and 0.5 g of N-hydroxysuccinimide to the naphthylacetic acid solution to react for 1 h, then fully mix with the chitosan oligosaccharide solution and react at room temperature for 14 h; after the reaction is completed, add dimethyl sulfoxide to fully precipitate the product, separate the product and wash it three times with anhydrous ethanol, and then dry it to obtain a naphthylacetic acid-chitosan oligosaccharide complex;

[0042] (2) using a naphthaleneacetic acid-chitosan oligosaccharide complex in water to prepare an 8 mg / mL naphthaleneacetic acid-chitosan oligosaccharide solution; dissolving 1.5 g of indolebutyric acid in ethanol, and gradually diluting with water to obtain a 5 g / L indolebutyric acid solution; adding the indolebutyric acid solution dropwise to the naphthaleneacetic acid-chitosan oligosaccharide solution while stirring, and continuing to stir for 1 hour after the addition is complete, evaporating the solvent, collecting the product and fully drying it to obtain a composite precursor;

[0043] (3) The composite precursor is mixed with 3 g of brassinolide, moistened with a 50% ethanol aqueous solution, ground to dryness, and dried to obtain a plant growth salt-resistant agent.

[0044] Embodiment 6:

[0045] (1) Dissolving 7 g of chitosan oligosaccharide in 1% glacial acetic acid solution to obtain a chitosan oligosaccharide solution; dissolving 1 g of naphthaleneacetic acid in hot water to obtain a naphthaleneacetic acid solution; adding 1 g of 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride and 0.5 g of N-hydroxysuccinimide to the naphthaleneacetic acid solution to react for 2 h, and then fully mixing with the chitosan oligosaccharide solution and reacting at room temperature for 14 h; after the reaction is completed, adding dimethyl sulfoxide to fully precipitate the product, separating the product and washing it three times with anhydrous ethanol, and drying it to obtain a naphthaleneacetic acid-chitosan oligosaccharide complex;

[0046] (2) using a naphthaleneacetic acid-chitosan oligosaccharide complex in water to prepare a 10 mg / mL naphthaleneacetic acid-chitosan oligosaccharide solution; dissolving 1.5 g of indolebutyric acid in ethanol, and gradually diluting with water to obtain a 10 g / L indolebutyric acid solution; adding the indolebutyric acid solution dropwise to the naphthaleneacetic acid-chitosan oligosaccharide solution while stirring, and continuing to stir for 2 hours after the addition is complete, evaporating the solvent, collecting the product and fully drying it to obtain a composite precursor;

[0047] (3) The composite precursor is mixed with 3 g of brassinolide, moistened with a 35% ethanol aqueous solution, ground to dryness, and dried to obtain a plant growth anti-salt agent.

[0048] Example sample test:

[0049] Rice saline soil experiment: The salt-resistant agent of Example 1 was used to prepare 100, 300, 500 and 700 times dilutions respectively, urea, potassium dihydrogen phosphate, calcium chloride and ammonium chloride were used as nutrients, and no application of the salt-resistant agent was used as a control. The rice seeds were soaked for 48 hours, and seedlings were raised in trays. Seedlings of uniform size and height were selected, and the plant height was observed 10 days after transplanting to the saline soil.

[0050] The results are as follows Figure 1 and Figure 2 As shown by Figure 2 It can be seen that the average plant height of the blank control with water-soaked seeds is the lowest, only 26 cm, the plant height of the 100-fold salt-resistant agent dilution is the highest, reaching 31.8 cm, the plant height of the 300-fold dilution is 28.3 cm, and the plant height of the 500-fold dilution is also close to 30 cm, reaching 29.2 cm; the plant height after 700-fold dilution reaches 27.4 cm, which is still higher than the control group, indicating that the salt-resistant agent of the present invention can improve the stress resistance of plants growing in saline soil.

[0051] Rice salt stress experiment: The salt-resistance agent of Example 1 was used, and 100, 300, 500 and 700 times dilutions were prepared respectively, urea, potassium dihydrogen phosphate, calcium chloride and ammonium chloride were used as nutrients, and no salt-resistance agent was used as a control. The rice was subjected to salt stress treatment with 0.4% sodium chloride solution and dilutions, and the rice seeds were soaked for 36 hours, and the saline soil was directly seeded to observe the emergence effect. After the emergence, the rice seeds were subjected to long-term high temperature and drought stress, and the emergence effect in the salt water was observed.

[0052] Depend on Figure 3 and Figure 4 It can be seen that the emergence rate of rice seeds treated with the salt-resistant agent of the present invention is significantly higher than that of the control group, and even if the salt-resistant agent is diluted 2000 times, the emergence rate is significantly higher. After long-term high temperature and drought stress, the rice seedlings in the saline soil of the control group almost did not survive, while the survival and growth of the group treated with the salt-resistant agent were also significantly better than those of the control group, among which the rice seedlings treated with the salt-resistant agent diluted 1000 times had the best survival.

[0053] It can be seen that the salt-resistant agent of the present invention can effectively improve the salt resistance of plants in the process of plant growth such as seed soaking, coating, transplanting and root soaking. The components of the salt-resistant agent are synergistic. After being diluted to a high multiple, a very small amount can produce a significant effect of improving the survival rate of plants, with low application cost and strong practicality.

[0054] For those skilled in the art, various other corresponding changes and deformations can be made according to the technical solutions and concepts described above, and all of these changes and deformations should fall within the protection scope of the claims of the present invention.

Claims

1. A plant growth salt-resistance agent, characterized in that: Calculated by weight, the invention comprises 50 to 100 parts of chitosan oligosaccharide as a carrier and 20 to 50 parts of brassinolide, 3 to 15 parts of naphthylacetic acid and 5 to 25 parts of indolebutyric acid which are complexed with the chitosan oligosaccharide.

2. The plant growth salt-resistance agent according to claim 1, characterized in that: Add water to make a 30-4000 times dilution.

3. A method for preparing the plant growth salt-resistant agent according to claim 1 or 2, characterized in that: The following steps are involved: a. Dissolve chitosan oligosaccharide in 0.5-2% glacial acetic acid solution to obtain chitosan oligosaccharide solution; dissolve naphthaleneacetic acid in hot water to obtain naphthaleneacetic acid solution; then, fully mix with the chitosan oligosaccharide solution and react at room temperature for 12-16 hours; after the reaction is completed, add dimethyl sulfoxide to fully precipitate the product, separate the product, and fully wash and dry it with ethanol to obtain a naphthaleneacetic acid-chitosan oligosaccharide complex; b. Place the naphthaleneacetic acid-chitosan oligosaccharide complex in water to prepare a 5-10 mg / mL naphthaleneacetic acid-chitosan oligosaccharide solution; preparing an indoleacetic acid solution; dropping the indolebutyric acid solution into the naphthylacetic acid-chitosan oligosaccharide solution while stirring, continuing to stir for 1 to 2 hours after the addition is completed, evaporating the solvent, collecting the product and fully drying it to obtain a composite precursor; c. Mix the composite precursor with brassinolide, moisten with an ethanol aqueous solution, grind to dryness, and obtain a plant growth anti-salt agent after drying.

4. The method for preparing the plant growth salt-resistance agent according to claim 3, characterized in that: The mass ratio of chitosan oligosaccharide to naphthylacetic acid in step a is 5-10:

1.

5. The method for preparing the plant growth salt-resistance agent according to claim 3, characterized in that: Before mixing with the chitosan oligosaccharide solution, the naphthylacetic acid solution is added with 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride and N-hydroxysuccinimide to react for 1 to 2 hours.

6. The method for preparing the plant growth salt-resistance agent according to claim 5, characterized in that: The mass ratio of the naphthylacetic acid to 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride is 1:0.8-1.5; the mass ratio of the 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride to N-hydroxysuccinimide is 1.5-3:

1.

7. The method for preparing the plant growth salt-resistance agent according to claim 3, characterized in that: The step of preparing the indoleacetic acid solution in step b is: dissolving indolebutyric acid in ethanol, and gradually diluting with water to obtain an indolebutyric acid solution.

8. The method for preparing the plant growth salt-resistance agent according to claim 3 or 7, characterized in that: The concentration of the indolebutyric acid solution is 1-10 g / L.

9. The method for preparing the plant growth salt-resistance agent according to claim 3, characterized in that: In step c, the volume ratio of ethanol to water in the ethanol aqueous solution is 1:0.5-2.

10. Use of the plant growth salt-resistant agent according to any one of claims 1 to 2 in seed soaking, coating, root soaking after transplanting or foliar spraying of plants.

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