Environment-friendly bioactive pesticide composition

Abstract

The invention belongs to the technical field of bioactive pesticides, and particularly relates to an environment-friendly bioactive pesticide composition. The environment-friendly bioactive pesticide composition is formed by compounding a first active ingredient and a second active ingredient, the first active ingredient is plant essential oil, the second active ingredient is plant essential oil, and the first active ingredient is plant essential oil. The second active component is polyoxin or cnidium lactone. The honeysuckle flower essential oil and the existing bioactive component polyoxin or cnidium lactone are compounded according to a certain mass ratio, the compounded combination formula shows a synergistic effect, compared with a single component, the prevention and treatment effect on crop diseases can be improved, the use of chemical agents can be reduced, and the cost is reduced. The honeysuckle flower essential oil, the polyoxin and the cnidium lactone are bioactive components, are low in residue and environment-friendly, and have important significance on development of biopesticide for preventing and treating crop diseases and green and sustainable development of agriculture.

Description

Technical Field

[0001] The invention belongs to the technical field of bioactive pesticides, and in particular relates to an environmentally friendly bioactive pesticide composition. Background Art

[0002] In the agricultural process, crops are often attacked by a variety of pests and diseases, which will affect the healthy growth of crops, and then affect their quality and yield. At present, chemical agents are mainly used to control pests and diseases on crops. Chemical agents have the advantages of convenient application and quick effect. However, in actual application, long-term use of chemical agents, especially single-component chemical agents, is prone to cause drug resistance, resulting in reduced efficacy of the agent. In addition, the large-scale use of chemical agents has problems of environmental pollution and pesticide residues. Biological pesticides have low residues, are environmentally friendly, and are in line with the green and sustainable development of agriculture, but the control effect of a single biologically active pesticide is limited. Different pesticides can be compounded, and the combination with synergistic effects can be screened to improve the control effect. Based on this, screening a compound formula of biological pesticides with synergistic effects is of great significance to agricultural development.

[0003] Honeysuckle essential oil is a volatile essential oil extracted from dried honeysuckle flowers. The prior art has reported that honeysuckle essential oil has a highly effective antibacterial effect and can induce the activity of plant defense-related enzymes CAT and SOD to improve plant disease resistance. The prior art also discloses a fungicide composition formed by compounding kasugamycin and honeysuckle essential oil. When used to prevent and control soil-borne diseases of crops, it can significantly improve the prevention effect of kasugamycin on a variety of soil-borne diseases on ginger, tomatoes, corn and cotton, and can effectively reduce the amount of chemical pesticides, reduce pesticide residues and environmental pollution; in addition, a fungicide composition formed by compounding boscalid and honeysuckle essential oil is disclosed. When used to prevent and control walnut diseases, it can significantly improve the prevention effect of boscalid on walnut leaf and fruit diseases, and can effectively reduce the amount of chemical pesticides, reduce pesticide residues and environmental pollution.

[0004] So far, there are no reports on the compounding of honeysuckle essential oil with existing bioactive ingredients such as polyoxin or osthole. Summary of the invention

[0005] The object of the present invention is to provide an environmentally friendly bioactive pesticide composition, which can improve the control effect of diseases, reduce the amount of chemical agents used, and reduce pesticide residues and environmental pollution compared with a single agent.

[0006] To achieve the above object, the present invention provides the following technical solutions:

[0007] An environmentally friendly bioactive pesticide composition is compounded by a first active ingredient and a second active ingredient, wherein the first active ingredient is plant essential oil; and the second active ingredient is polyoxin or osthole.

[0008] Specifically, the plant essential oil is honeysuckle essential oil.

[0009] Furthermore, the mass ratio of the honeysuckle essential oil to polyoxin is 1-20:20-1.

[0010] Furthermore, the mass ratio of the honeysuckle essential oil to osthole is 1-3:8-1.

[0011] The present invention also provides the use of the environmentally friendly bioactive pesticide composition in the preparation of biopesticides.

[0012] The present invention also provides application of the environmentally friendly bioactive pesticide composition in preventing and controlling crop diseases.

[0013] Compared with the prior art, the present invention has the following beneficial effects:

[0014] The invention compounds honeysuckle essential oil with existing biologically active ingredients such as polyoxin or osthole in a certain mass ratio, and the compounded combination formula shows a synergistic effect. Compared with a single ingredient, the control effect on crop diseases can be improved, and the use of chemical agents can be reduced. The honeysuckle essential oil, polyoxin and osthole are all biologically active ingredients, have low residues, are environmentally friendly, and are of great significance to the development of biological pesticides for controlling crop diseases and the green and sustainable development of agriculture. DETAILED DESCRIPTION

[0015] Based on the embodiments of the present invention, all other embodiments obtained by technicians in this field without making any creative work shall fall within the scope of protection of the present invention.

[0016] Example: Indoor toxicity test of single and compound agents against wheat sheath blight

[0017] 1. Test strains

[0018] Rizoctonia solani was isolated from infected wheat plants and maintained on PDA medium.

[0019] 2. Test culture medium

[0020] PDA medium: 200 g potato, 20 g glucose, 15 g agar, distilled water to 1000 mL, natural pH.

[0021] 2. Test reagents

[0022] 99% honeysuckle essential oil (Jiangxi Baolin Natural Flavor Co., Ltd.), 34% polyoxin technical (Shandong Rushan Hanwei Biotechnology Co., Ltd.), 10% osthole technical (Kangxin Biotechnology Co., Ltd.)

[0023] 3. Test methods

[0024] The test agent was prepared into a single stock solution with an active ingredient concentration of 10,000 mg / L. At the same time, multiple groups of ratios were set according to the preliminary test. The single stock solution and the ratio mixture were diluted to 5 mass concentrations with 0.1% Tween-80 aqueous solution, and then the mycelium growth rate method was used to determine the toxicity of the single agent and the ratio mixture to the test strain. The specific contents are as follows:

[0025] The drug solution and the PDA medium that was melted and cooled to 50°C were mixed in a volume ratio of 1:9, and then poured into a culture dish (9 cm in diameter), 10 mL per dish, and cooled to form a drug-containing plate; and a plate made by mixing 0.1% Tween-80 aqueous solution and PDA medium was used as a blank control. Each treatment was repeated 3 times, and each time there were 4 plates.

[0026] Inoculate the test strain on PDA medium, culture at 25℃ for 3 days, cut a 6mm diameter bacterial cake along the edge of the colony with the same diameter with a hole puncher, inoculate the bacterial cake on the center of the drug-containing plate and the blank control plate, and inoculate one bacterial cake on each plate. Cover the plate and continue to culture at 25℃.

[0027] Observe the growth of the test strain regularly. When the diameter of the colony on the blank control plate grows to 2 / 3 of the diameter of the culture dish, measure the colony diameter with a caliper. Measure the diameter of each colony vertically once using the cross method, take the average value, and calculate the inhibition rate of mycelium growth under different treatments based on the measurement results.

[0028]

[0029] 4. Statistical Analysis

[0030] The EC of each agent was calculated by regression analysis based on the logarithmic value of each agent concentration and the corresponding probability value of mycelium growth inhibition rate. 50 The co-toxicity coefficient (CTC) of the mixture was calculated according to Sun Yunpei's method.

[0031]

[0032] In the above formula: ATI--the toxicity index of the mixture; S--the EC of the standard agent 50 , the unit is mg / L; M--EC of the mixture 50 , unit is mg / L.

[0033] TTI=TI A ×P A +TI B ×P B

[0034] In the above formula: TTI-theoretical toxicity index of the mixture; TI A --Toxicity index of A agent; P A --The percentage of agent A in the mixture, in percentage (%); TI B --Toxicity index of agent B; P B --The percentage of agent B in the mixture, in percentage (%).

[0035]

[0036] In the above formula: CTC--co-toxicity coefficient; ATI--actual toxicity index of the mixture; TTI--theoretical toxicity index of the mixture.

[0037] 5. Measurement results

[0038] The synergistic effect of the drug was evaluated based on the calculated co-toxicity coefficient (CTC). CTC≤80 was antagonistic, 80<CTC<120 was additive, and CTC≥120 was synergistic. The results are shown in Table 1-2.

[0039] Table 1 Regression analysis results of honeysuckle essential oil and polyoxin combined with Rhizoctonia solani

[0040]

[0041] Table 2 Evaluation results of the efficacy of honeysuckle essential oil and polyoxin combined against Rhizoctonia solani

[0042]

[0043] It can be seen from the test results in Table 1-2 that when honeysuckle essential oil and polyoxin are compounded in the mass ratio range of 1-20:20-1, they show a synergistic effect on the wheat sheath blight fungus Rhizoctonia solani. Compared with a single ingredient, it can improve the prevention and control effect of crop diseases and reduce the use of chemical agents.

[0044] Table 3 Regression analysis results of honeysuckle essential oil and osthole compound on Rhizoctonia solani

[0045]

[0046] Table 4 The efficacy evaluation results of honeysuckle essential oil and osthole against Rhizoctonia solani

[0047]

[0048] It can be seen from the test results in Table 3-4 that when honeysuckle essential oil and polyoxin are compounded in the mass ratio range of 1-20:20-1, they show a synergistic effect on the wheat sheath blight fungus Rhizoctonia solani. Compared with a single ingredient, it can improve the prevention and control effect of crop diseases and reduce the use of chemical agents.

[0049] In summary, the present invention compounds honeysuckle essential oil with existing biologically active ingredients such as polyoxin or osthole in a certain mass ratio, and the combined formula after compounding shows a synergistic effect. Compared with a single ingredient, the prevention and control effect of crop diseases can be improved, and the use of chemical agents can be reduced. Moreover, honeysuckle essential oil, polyoxin and osthole are all biologically active ingredients, have low residues, and are environmentally friendly, which are of great significance to the development of biological pesticides for preventing and controlling crop diseases and the green and sustainable development of agriculture.

Claims

1. An environmentally friendly bioactive pesticide composition, characterized in that: The environmentally friendly bioactive pesticide composition is compounded by a first active ingredient and a second active ingredient, wherein the first active ingredient is plant essential oil; and the second active ingredient is polyoxin or osthole.

2. The environmentally friendly biologically active pesticide composition according to claim 1, characterized in that: The plant essential oil is honeysuckle essential oil.

3. The environmentally friendly biologically active pesticide composition according to claim 2, characterized in that: The mass ratio of the honeysuckle essential oil to polyoxin is 1-20:20-1.

4. The environmentally friendly biologically active pesticide composition according to claim 2, characterized in that: The mass ratio of the honeysuckle essential oil to osthole is 1-3:8-1.

5. Use of the environmentally friendly bioactive pesticide composition according to any one of claims 1 to 4 in the preparation of biopesticides.

6. Use of the environmentally friendly bioactive pesticide composition according to any one of claims 1 to 4 in preventing and controlling crop diseases.

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