Vegetable oil acaricide as well as preparation method and application thereof

Abstract

The invention provides a vegetable oil acaricide as well as a preparation method and application thereof, and belongs to the technical field of mite prevention and control. The plant oil miticide provided by the invention comprises 0.1-80 parts of plant oil, 1-30 parts of an emulsifier and 1-99 parts of a solvent, the vegetable oil is selected from any one or more of coriander oil, sandalwood oil, perilla seed oil, shea butter and castor oil. The invention also provides a preparation method and application of the plant oil miticide. The plant oil acaricide is low in raw material formula price, simple in preparation method, remarkable in acarid control effect and environmentally friendly.

Description

Technical Field

[0001] The invention belongs to the technical field of mite control, and particularly relates to a vegetable oil acaricide and a preparation method and application thereof. Background Art

[0002] Mites are a type of arthropod that causes serious damage to agriculture, horticulture and other fields. In agricultural production, the most common and extremely harmful mites are spider mites, citrus spider mites and two-spotted spider mites. Spider mites widely infest a variety of vegetable crops, such as tomatoes, eggplants, peppers, etc., and feed on leaf juice. The affected leaves initially show small yellow-white spots. As the mites continue to feed and reproduce, these spots gradually expand and connect into pieces, causing the leaves to lose their green color, turn yellow, and eventually fall off in large numbers, seriously affecting the photosynthesis and growth and development of vegetables, and causing a significant decrease in vegetable yield and quality. The citrus spider mite mainly harms citrus fruit trees, including citrus leaves, young shoots and fruits. After the leaves are infected, the surface is covered with gray-white chlorotic spots. In severe cases, the entire leaf turns gray-white and the texture becomes hard, and falls off prematurely, which has a catastrophic impact on the yield and fruit quality of citrus. The two-spotted spider mite has a wide host range. In addition to agricultural crops, it also poses a threat to a variety of flowers, fruit trees and economic crops, causing yellow spots and scorch on plant leaves, poor development of flowers or even withering, seriously damaging the ornamental and economic value of the plants.

[0003] Mites have the characteristics of fast reproduction speed. Under suitable temperature and humidity conditions, the population can grow exponentially in a short period of time. At present, chemical miticides are one of the main means of controlling mites. Common chemical miticides on the market include organophosphorus, pyrethroids, pyridaben, etc. Although these miticides have certain miticide effects, long-term use can easily lead to serious drug resistance in mites. At the same time, they pollute the environment and have a high toxicity risk to beneficial insects and mammals. Biological miticide methods mainly include using natural enemies such as predatory mites and insect pathogenic fungi to control the mite population. In the actual agricultural production environment, their survival and reproduction are restricted by many factors, and it is often difficult to quickly control the number of mites during the peak period of mite outbreaks. Physical control methods such as high temperature treatment and ultraviolet irradiation have limitations in practical applications. It is extremely difficult to implement for large areas of farmland and orchards, and may cause damage to the plants themselves. It is also difficult to completely kill the mites hidden on the back of the leaves and inside the plants. Therefore, it is urgent to develop a new, efficient and environmentally friendly miticide to effectively deal with the harm of mites. Summary of the invention

[0004] In order to solve the problems existing in the prior art, the purpose of the present invention is to provide a plant oil acaricide, which has a simple production process, low cost, good acaricide effect, and is safe and environmentally friendly.

[0005] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0006] The invention provides a vegetable oil acaricide, which comprises the following raw materials by weight: 0.1 to 80 parts of vegetable oil, 1 to 30 parts of emulsifier, and 1 to 99 parts of solvent; the vegetable oil is selected from any one or more of coriander oil, sandalwood oil, perilla seed oil, shea butter and castor oil.

[0007] Preferably, the emulsifier includes any one or more of polyoxyethylene polyoxypropylene block copolymer, fatty alcohol polyoxyethylene ether, polyoxyethylene alkyl ester, alkylphenol polyoxyethylene ether, sorbitol polyoxyethylene ether, anhydrous sorbitan polyoxyethylene ether, alkylphenol formaldehyde condensate polyoxyethylene ether, sorbitan monooleate, fatty alcohol and ethylene oxide condensate, alkylbenzene sulfonate, fatty alcohol polyoxyethylene ether sodium sulfosuccinate, castor oil polyoxyethylene ether, lignin sulfonate, naphthalene and alkylnaphthalene sulfonate formaldehyde condensate, carboxymethyl cellulose, polycarboxylate dispersant, sodium tripolyphosphate, alkylnaphthalene sulfonic acid condensate sodium salt, calcium dodecylbenzene sulfonate and tristyrylphenol polyoxyethylene ether.

[0008] Preferably, the solvent includes an organic solvent and / or an inorganic solvent; the organic solvent includes any one or more of methyl oleate, xylene, cyclohexanone, solvent oil 150#, solvent oil 200# and methylated soybean oil; and the inorganic solvent is water.

[0009] Preferably, the plant oil acaricide further comprises 1 to 99 parts of a co-surfactant and 1 to 20 parts of an antifreeze agent.

[0010] The present invention also provides a method for preparing the plant oil acaricide, comprising the following steps: mixing various raw material components in proportion to prepare the plant oil acaricide.

[0011] The present invention also provides the use of the plant oil acaricide in preparing products for preventing and controlling mites.

[0012] The present invention also provides a method for preventing and controlling mites in the field, comprising the following steps: spraying the vegetable oil miticide on crops.

[0013] Preferably, the vegetable oil acaricide is diluted 1 to 2000 times.

[0014] Preferably, the mites include Tetranychus cinnabarinus, Panonychus citri and Tetranychus urticae.

[0015] Preferably, the plant oil miticide kills adult mites, nymphs, young mites and mite eggs.

[0016] Compared with the prior art, the technical solution of the present invention has the following beneficial effects:

[0017] The present invention provides a plant oil acaricide based on plant oil, which has simple production process, low cost, high acaricide effect, safety and environmental protection. The present invention proves that the plant oil acaricide can effectively kill adult mites, nymphs, young mites and eggs of Tetranychus cinnabarinus, Panonychus citri and Tetranychus urticae through indoor egg-killing and adult mite-killing experiments and field experiments, and will not pollute the environment. DETAILED DESCRIPTION

[0018] The present invention provides a vegetable oil acaricide, which comprises the following raw materials, measured by weight: 0.1 to 80 parts of vegetable oil, 1 to 30 parts of emulsifier, and 1 to 99 parts of solvent; the raw materials preferably comprise: 30 to 60 parts of vegetable oil, 5 to 25 parts of emulsifier, and 3 to 65 parts of solvent; the raw materials more preferably comprise: 40 to 50 parts of vegetable oil, 20 parts of emulsifier, and 40 to 50 parts of solvent.

[0019] The vegetable oil of the present invention is selected from any one or more of coriander oil, sandalwood oil, perilla seed oil, shea butter and castor oil. The present invention can use any one of the vegetable oils as an active ingredient, or a combination of two or more of the vegetable oils as an active ingredient, and the present invention does not limit the ratio of the combination.

[0020] The emulsifier of the present invention includes any one or more of polyoxyethylene polyoxypropylene block copolymer, fatty alcohol polyoxyethylene ether, polyoxyethylene alkyl ester, alkylphenol polyoxyethylene ether, sorbitol polyoxyethylene ether, anhydrous sorbitol polyoxyethylene ether, alkylphenol formaldehyde condensate polyoxyethylene ether, sorbitol monooleate, fatty alcohol and ethylene oxide condensate, alkylbenzene sulfonate, fatty alcohol polyoxyethylene ether sodium sulfosuccinate, castor oil polyoxyethylene ether, lignin sulfonate, naphthalene and alkylnaphthalene sulfonate formaldehyde condensate, carboxymethyl cellulose, polycarboxylate dispersant, sodium tripolyphosphate, alkylnaphthalene sulfonic acid polycondensate sodium salt, calcium dodecylbenzene sulfonate and tristyrylphenol polyoxyethylene ether. The present invention can select any one of the emulsifiers, or select a combination of two or more of the components as the emulsifier, and the present invention does not limit the proportion of the combination.

[0021] The solvent of the present invention includes an organic solvent and / or an inorganic solvent; the organic solvent includes any one or more of methyl oleate, xylene, cyclohexanone, solvent oil 150#, solvent oil 200# and methylated soybean oil; the inorganic solvent is water. The present invention can select any one of the solvents, or a combination of two or more of the components as an emulsifier. The present invention does not limit the ratio of the combination, and the selection of the specific solvent is determined according to the dosage form of the prepared plant oil acaricide.

[0022] The plant oil acaricide of the present invention further comprises 1 to 99 parts of a cosurfactant and 1 to 20 parts of an antifreeze agent; the cosurfactant is preferably 2 to 80 parts, more preferably 5 to 10 parts; the antifreeze agent is preferably 2 to 15 parts, more preferably 5 to 10 parts.

[0023] The cosurfactant of the present invention can be selected by those skilled in the art according to actual use requirements, and the cosurfactant can be nonionic or ionic. In some embodiments of the present invention, the cosurfactant that can be used includes but is not limited to ethoxylated castor oil, sodium lauryl sulfate, saponin, ethoxylated alcohol, ethoxylated fatty ester, alkoxylated glycol, ethoxylated fatty acid, carboxylated alcohol, carboxylic acid, fatty acid, ethoxylated alkylphenol, fatty ester, sodium dodecyl sulfide, other cosurfactants based on fatty acid, other natural or synthetic cosurfactants, and combinations thereof. The present invention can select any one of the cosurfactants, or can select two or more of the components as the cosurfactant, and the present invention does not limit the ratio of the combination. The antifreeze agent of the present invention includes but is not limited to ethylene glycol, propylene glycol, and glycerol. Those skilled in the art select appropriate cosurfactants according to relevant applications and conditions of use.

[0024] The formulation of the plant oil acaricide of the present invention is any one of an emulsifiable concentrate (EC), a microemulsion (ME) and an emulsion in water (EW). The formulation of the plant oil acaricide of the present invention can also be any one of a soluble powder (SP), a soluble granule (SG), a soluble solution (SL), a dispersible liquid (DC), a microcapsule suspension (CS), a seed treatment liquid (LS) and an aerosol (AE).

[0025] The present invention also provides a method for preparing the plant oil acaricide, comprising the following steps: mixing various raw material components in proportion to prepare the plant oil acaricide.

[0026] When the formulation of the vegetable oil acaricide is an emulsifiable concentrate, the raw materials of the vegetable oil acaricide include vegetable oil, an emulsifier and an organic solvent, and the preparation method includes the following steps: uniformly mixing the vegetable oil, the emulsifier and the organic solvent.

[0027] When the formulation of the vegetable oil acaricide is a microemulsion, the vegetable oil acaricide comprises vegetable oil, an emulsifier, an organic solvent and water (inorganic solvent), and the preparation method comprises the following steps: stirring and mixing the vegetable oil, the emulsifier and the organic solvent uniformly to obtain an oil phase, slowly adding water to the oil phase while stirring, and after completing the phase transition, a microemulsion is obtained. When a cosurfactant and an antifreeze agent are added to the microemulsion, the preparation method comprises the following steps: stirring and mixing the vegetable oil, the emulsifier, the organic solvent and the cosurfactant uniformly to obtain an oil phase, mixing water and the antifreeze agent to obtain an aqueous phase, slowly adding the aqueous phase to the oil phase while stirring, and after completing the phase transition, a microemulsion is obtained.

[0028] When the formulation of the vegetable oil acaricide is an aqueous emulsion, the vegetable oil acaricide comprises vegetable oil, an emulsifier, an organic solvent and water (inorganic solvent), and the preparation method comprises the following steps: stirring and mixing the vegetable oil, the emulsifier and the organic solvent to obtain an oil phase, slowly adding water to the oil phase while stirring, turning on a high-speed shearing machine, and shearing the oil phase uniformly to obtain an aqueous emulsion. When a co-surfactant and an antifreeze agent are added to the aqueous emulsion, the preparation method comprises the following steps: stirring and mixing the vegetable oil, the emulsifier, the organic solvent and the co-surfactant to obtain an oil phase, mixing water and the antifreeze agent to obtain an aqueous phase, slowly adding the aqueous phase to the oil phase while stirring, turning on a high-speed shearing machine, and shearing the oil phase uniformly to obtain an aqueous emulsion.

[0029] When it is other dosage forms, those skilled in the art can prepare it according to conventional dosage form preparation methods.

[0030] The present invention also provides the use of the above-mentioned plant oil acaricide in preparing products for controlling mites, including Tetranychus cinnabarinus, Panonychus citri and Tetranychus urticae. The plant oil acaricide of the present invention kills adult mites, nymphs, young mites and mite eggs.

[0031] The present invention also provides a method for controlling mites in the field, comprising the following steps: spraying the above-mentioned vegetable oil acaricide on crops. As an optional embodiment, the present invention uniformly sprays the above-mentioned vegetable oil acaricide on the crop parts to be controlled until small water droplets are formed. The crop parts to be controlled in the present invention include but are not limited to crop leaves, main branches, branches, flowers, and fruits. The present invention does not apply other insecticides, fungicides, or acaricides during the control period. The mites described in the present invention include cinnabarinus spider mites, citrus panonychus mites, and two-spotted spider mites. The vegetable oil acaricide described in the present invention kills adult mites, nymphs, young mites, and mite eggs.

[0032] The plant oil acaricide of the present invention can be sprayed directly or diluted with water for spraying, and the dilution multiple is 1 to 2000 times, preferably 50 to 1000 times, and more preferably 100 to 600 times. The present invention can select a dilution-free formulation or determine the dilution multiple according to the specific control effect.

[0033] The plant oil acaricide of the present invention can be used for preventing and controlling Tetranychus, and / or Tenebriocaridae, and / or Gall Mite, and / or Tarsonema, and / or Pygemycin, and / or Leaf-clawed Mite, and / or Carnivorous Mite, and / or Sarcoptes, and / or Psoralea, and / or Dust Mite. The pests include but are not limited to one or more of the following: white-clawed pine mite, larch small-clawed mites, carmine small-clawed mites, white spider mites, white wax spider mites, camellia spider mites, vegetable spider mites, bean spider mites, tea yellow mites, two-spotted spider mites, cinnabarin spider mites, citrus split-clawed mites, bamboo split-clawed mites, long-clawed mites, fig synonychus mites, citrus synonychus mites, citrus panonychus mites, Carson's panonychus mites, bamboo heteroonychus mites, wuyi heteroonychus mites, bamboo elk mites, Georgia monoonychus mites, spiked secondary claw mites, hawthorn double-clawed mites, scabies mites, flour mites, and dust mites.

[0034] The technical solutions in the present invention will be described clearly and completely below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0035] In a specific embodiment of the present invention, coriander oil and shea butter are purchased from Shaanxi Panier Biotechnology Co., Ltd.; sandalwood oil is purchased from Shanghai McLean Biochemical Technology Co., Ltd.; perilla seed oil and castor oil are purchased from Jiangxi Xuesong Natural Medicinal Oil Co., Ltd.; soybean oil, litsea cubeba oil, peppermint oil, phellodendron chinense oil, fennel oil, cinnamon oil, corn oil, turpentine oil, atractylodes macrocephala oil, black pepper oil, and wintergreen oil are purchased from Shaanxi Panier Biotechnology Co., Ltd.; linseed oil, grape seed oil, wheat germ oil, palm oil, almond oil, rice bran oil, red camphor oil, safflower seed oil, pumpkin seed oil, macadamia nut oil, angelica dahurica oil, Eucalyptus oil, mustard oil, and sunflower oil were purchased from Jiangxi Xuesong Natural Medicinal Oil Co., Ltd.; sweet birch oil, spearmint oil, and nutmeg oil were purchased from Shanghai MacLean Biochemical Technology Co., Ltd.; mineral oil (trade name: Green Ying; manufacturer: Korea Hanyou Aiskai Yitsu Co., Ltd.) was purchased from Feixiang Agricultural Materials Official Store Taobao Store; Ethiocarb (trade name: Baozhuo; manufacturer: Shenyang Kechuang Chemicals Co., Ltd.) was purchased from the Zhongbao Green Agriculture Group Taobao Store of the Institute of Plant Protection, Chinese Academy of Agricultural Sciences; Spirocyclopentane (trade name: Mite Wei; manufacturer: Yongnong Bioscience Co., Ltd.) was purchased from Feixiang Agricultural Materials Official Store Taobao Store.

[0036] In the following embodiments, unless otherwise specified, all of them are conventional methods.

[0037] Unless otherwise specified, the materials and reagents used in the following examples can be obtained from commercial sources.

[0038] Example 1

[0039] A vegetable oil acaricide, comprising the following raw materials: 6g vegetable oil, 2.5g emulsifier and 1.5g solvent; the vegetable oil is coriander oil; the emulsifier is 2g castor oil polyoxyethylene ether EL-20 emulsifier + 0.5g calcium dodecylbenzene sulfonate; and the solvent is ethanol.

[0040] Preparation method: vegetable oil and emulsifier are mixed, and then a solvent is added to prepare the vegetable oil acaricide.

[0041] Example 2

[0042] A plant oil acaricide, comprising the following raw materials: 6g of plant oil, 2.5g of emulsifier, and 1.5g of solvent; the plant oil is sandalwood oil; the emulsifier is 2g of castor oil polyoxyethylene ether EL-20 emulsifier + 0.5g of calcium dodecylbenzene sulfonate; and the solvent is ethanol.

[0043] Preparation method: vegetable oil and emulsifier are mixed, and then a solvent is added to prepare the vegetable oil acaricide.

[0044] Example 3

[0045] A plant oil acaricide, comprising the following raw materials: 6g of plant oil, 2.5g of emulsifier, and 1.5g of solvent; the plant oil is perilla seed oil; the emulsifier is 2g of castor oil polyoxyethylene ether EL-20 emulsifier + 0.5g of calcium dodecylbenzene sulfonate; and the solvent is ethanol.

[0046] Preparation method: vegetable oil and emulsifier are mixed, and then a solvent is added to prepare the vegetable oil acaricide.

[0047] Example 4

[0048] A vegetable oil acaricide, comprising the following raw materials: 6g vegetable oil, 2.5g emulsifier and 1.5g solvent; the vegetable oil is shea butter; the emulsifier is 2g castor oil polyoxyethylene ether EL-20 emulsifier + 0.5g calcium dodecylbenzene sulfonate; and the solvent is ethanol.

[0049] Preparation method: vegetable oil and emulsifier are mixed, and then a solvent is added to prepare the vegetable oil acaricide.

[0050] Example 5

[0051] A vegetable oil acaricide, comprising the following raw materials: 6g vegetable oil, 2.5g emulsifier and 1.5g solvent; the vegetable oil is castor oil; the emulsifier is 2g castor oil polyoxyethylene ether EL-20 emulsifier + 0.5g calcium dodecylbenzene sulfonate; and the solvent is ethanol.

[0052] Preparation method: vegetable oil and emulsifier are mixed, and then a solvent is added to prepare the vegetable oil acaricide.

[0053] Example 6

[0054] A vegetable oil acaricide (emulsifiable concentrate), the raw materials of which are: 4g of vegetable oil, 2g of emulsifier, and 4g of solvent; the vegetable oil is castor oil; the emulsifier is fatty alcohol polyoxyethylene ether (AEO-09); and the solvent is methyl oleate.

[0055] Preparation method: vegetable oil and emulsifier are mixed, and then a solvent is added to prepare the vegetable oil acaricide.

[0056] Example 7

[0057] A vegetable oil acaricide (microemulsion), the raw materials are: 4g vegetable oil, 2g emulsifier, 4g solvent; the vegetable oil is castor oil; the emulsifier is fatty alcohol polyoxyethylene ether (AEO-09); the solvent is 1g ethanol + 3g water.

[0058] Preparation method: stir and mix vegetable oil, emulsifier and ethanol evenly to obtain oil phase, slowly add water into the oil phase while stirring, and after phase inversion, a microemulsion is obtained.

[0059] Example 8

[0060] A vegetable oil acaricide (water emulsion), the raw materials of which are: 4g vegetable oil, 2g emulsifier, 4g solvent; the vegetable oil is castor oil; the emulsifier is fatty alcohol polyoxyethylene ether (AEO-09); and the solvent is water.

[0061] Preparation method: stir and mix the vegetable oil and emulsifier evenly to obtain the oil phase, slowly add water to the oil phase while stirring, start a high-speed shearing machine, and after uniform shearing, obtain the water emulsion.

[0062] Example 9

[0063] A vegetable oil acaricide (emulsifiable concentrate), the raw materials of which are: 6g of vegetable oil, 2.5g of emulsifier and 1.5g of solvent; the vegetable oil is sandalwood oil; the emulsifier is sorbitol polyoxyethyl ether; and the solvent is methyl oleate.

[0064] Preparation method: vegetable oil and emulsifier are mixed, and then a solvent is added to prepare the vegetable oil acaricide.

[0065] Example 10

[0066] A vegetable oil acaricide (emulsifiable concentrate), the raw materials of which are: 5g vegetable oil, 2g emulsifier and 3g solvent; the vegetable oil is perilla seed oil; the emulsifier is fatty alcohol polyoxyethylene ether; and the solvent is methylated soybean oil.

[0067] Preparation method: vegetable oil and emulsifier are mixed, and then a solvent is added to prepare the vegetable oil acaricide.

[0068] Embodiment 11

[0069] A vegetable oil acaricide (emulsifiable concentrate), the raw materials of which are: 6g of vegetable oil, 3g of emulsifier and 1g of solvent; the vegetable oil is shea butter; the emulsifier is sorbitol polyoxyethyl ether; and the solvent is 150# solvent oil.

[0070] Preparation method: vegetable oil and emulsifier are mixed, and then a solvent is added to prepare the vegetable oil acaricide.

[0071] Example 12

[0072] A vegetable oil acaricide (emulsifiable concentrate), the raw materials of which are: 5g vegetable oil, 2.5g emulsifier and 2.5g solvent; the vegetable oil is coriander oil; the emulsifier is lignin sulfonate; and the solvent is 200# solvent oil.

[0073] Preparation method: vegetable oil and emulsifier are mixed, and then a solvent is added to prepare the vegetable oil acaricide.

[0074] Embodiment 13

[0075] A vegetable oil acaricide (microemulsion), the raw materials of which are: 3g vegetable oil, 1.5g emulsifier, 5.5g solvent; the vegetable oil is coriander oil; the emulsifier is alkylphenol polyoxyethylene ether; the solvent is 1g ethanol + 4.5g water.

[0076] Preparation method: stir and mix vegetable oil, emulsifier and ethanol evenly to obtain oil phase, slowly add water into the oil phase while stirring, and after phase inversion, a microemulsion is obtained.

[0077] Embodiment 14

[0078] A plant oil acaricide (microemulsion), the raw materials are: 3g plant oil, 1.5g emulsifier, 5.5g solvent; the plant oil is sandalwood oil; the emulsifier is tristyrylphenol polyoxyethylene ether; the solvent is 0.5g ethanol + 5g water.

[0079] Preparation method: stir and mix vegetable oil, emulsifier and ethanol evenly to obtain oil phase, slowly add water into the oil phase while stirring, and after phase inversion, a microemulsion is obtained.

[0080] Embodiment 15

[0081] A vegetable oil acaricide (water emulsion), the raw materials of which are: 4g vegetable oil, 2g emulsifier and 4g solvent; the vegetable oil is shea butter; the emulsifier is fatty alcohol polyoxyethylene ether (AEO-09); and the solvent is water.

[0082] Preparation method: stir and mix the vegetable oil and emulsifier evenly to obtain the oil phase, slowly add water to the oil phase while stirring, start a high-speed shearing machine, and after uniform shearing, obtain the water emulsion.

[0083] Example 16

[0084] A vegetable oil acaricide (water emulsion), the raw materials of which are: 4g vegetable oil, 1.5g emulsifier, 4.5g solvent; the vegetable oil is perilla seed oil; the emulsifier is fatty alcohol polyoxyethylene ether (AEO-09); and the solvent is water.

[0085] Preparation method: stir and mix the vegetable oil and emulsifier evenly to obtain the oil phase, slowly add water to the oil phase while stirring, start a high-speed shearing machine, and after uniform shearing, obtain the water emulsion.

[0086] Embodiment 17

[0087] A plant oil acaricide (microemulsion), the raw materials of which are: 3g of plant oil, 2g of emulsifier, 0.2g of co-surfactant, 0.5g of antifreeze agent, and 4.3g of solvent; the plant oil is perilla seed oil; the emulsifier is sodium tripolyphosphate; the co-surfactant is diacetone alcohol; the antifreeze agent is glycerol; and the solvent is 0.2g of methyl oleate + 4.1g of water.

[0088] Preparation method: stir and mix vegetable oil, emulsifier, co-surfactant and methyl oleate to obtain oil phase, mix water and antifreeze agent to obtain water phase, slowly add water phase into oil phase while stirring, and obtain microemulsion after phase inversion.

[0089] Embodiment 18

[0090] A vegetable oil acaricide (microemulsion) comprises the following raw materials: 3 g vegetable oil, 1.5 g emulsifier, 0.2 g cosurfactant, 0.5 g antifreeze agent and 4.8 g solvent; the vegetable oil is shea butter; the emulsifier is carboxymethyl cellulose; the cosurfactant is n-butanol; the antifreeze agent is ethylene glycol; and the solvent is 0.3 g cyclohexanone + 4.5 g water.

[0091] Preparation method: stir and mix vegetable oil, emulsifier, co-surfactant and cyclohexanone evenly to obtain oil phase, mix water and antifreeze agent to obtain water phase, slowly add the water phase into the oil phase while stirring, and after phase inversion, a microemulsion is obtained.

[0092] Embodiment 19

[0093] A vegetable oil acaricide (water emulsion), the raw materials of which are: 4g vegetable oil, 2g emulsifier, 0.5g antifreeze agent, and 3.5g solvent; the vegetable oil is coriander oil; the emulsifier is fatty alcohol polyoxyethylene ether (AEO-09); the antifreeze agent is ethylene glycol; and the solvent is 0.2g solvent oil 150#+3.3g water.

[0094] Preparation method: stir and mix vegetable oil, emulsifier and solvent oil 150# evenly to obtain oil phase, mix water and antifreeze agent to obtain water phase, slowly add water phase into oil phase while stirring, start high-speed shearing machine, and obtain water emulsion after even shearing.

[0095] Embodiment 20

[0096] A vegetable oil acaricide (water emulsion), the raw materials are: 3g vegetable oil, 2g emulsifier, 0.5g antifreeze agent, 4.5g solvent; the vegetable oil is sandalwood oil; the emulsifier is sorbitan polyoxyethylene ether; the antifreeze agent is ethylene glycol; the solvent is 0.2g xylene + 4.3g water.

[0097] Preparation method: stir and mix vegetable oil, emulsifier and xylene evenly to obtain oil phase, mix water and antifreeze agent to obtain water phase, slowly add water phase into oil phase while stirring, start high-speed shearing machine, and obtain water emulsion after even shearing.

[0098] Test Example 1

[0099] Indoor acaricidal activity test on Tetranychus cinnabarinus, Panonychus citri and Tetranychus urticae:

[0100] 1. Preparation of test reagents:

[0101] The vegetable oil acaricides of Examples 1 to 5 were diluted 600 times with clean water respectively.

[0102] The vegetable oils in Example 1 were replaced with soybean oil, sweet birch oil, spearmint oil, nutmeg oil, litsea cubeba oil, peppermint oil, phellodendron chinense oil, fennel oil, cinnamon oil, corn oil, turpentine oil, atractylodes macrocephala oil, black pepper oil, wintergreen oil, linseed oil, grape seed oil, wheat germ oil, palm oil, almond oil, rice bran oil, red camphor oil, safflower seed oil, pumpkin seed oil, macadamia oil, turpentine oil, eucalyptus oil, mustard oil, sunflower seed oil, and angelica dahurica oil to prepare vegetable oil acaricides; each of the obtained vegetable oil acaricides was diluted 600 times with clean water as a control group of vegetable oil acaricides.

[0103] The vegetable oil in Example 1 was replaced with ethanol and diluted 600 times with clean water to serve as a solvent control group.

[0104] Mineral oil ("Green") was diluted 1000 times with clean water and used as the mineral oil control group.

[0105] Ethiophanate-methyl ("Baozhuo") was diluted 2000 times with clean water and used as the ethiophanate-methyl control group.

[0106] Clear water served as the blank control group.

[0107] 2. Experimental method (slide method):

[0108] (1) Sticking insects: Stick a 4 cm long double-sided tape on one end of a glass slide. Use a small brush to gently pick up a mite and stick its back gently on the tape. Be careful not to stick the mite's legs, antennae, and mouthparts. Stick at least 40 mites to each glass slide. Arrange them neatly and then place them in a clean culture dish. Put filter paper in it, keep the filter paper moist, and cover the dish with a lid.

[0109] (2) Microscopic examination: After 2 hours, observe each insect under a stereomicroscope. If there are any dead insects, remove them and cross them out. If there are fewer than 40 insects, the number must be replenished.

[0110] (3) Soaking the glass slide: Dip one end of the glass slide with healthy mites into the test agent and gently shake the glass slide. Take it out after immersion for 5 seconds and absorb the excess solution with absorbent paper. Then place the glass slide in a culture dish, dry the solution, cover the dish, and culture it at 25℃.

[0111] (4) Results survey

[0112] After 24, 48, and 72 hours, the number of dead mites was recorded under a stereomicroscope. The mites were gently touched with a small brush and no reaction was considered dead.

[0113] The calculation method refers to the industry standard NY / T 1154.12-2008 spider mite slide dipping method. According to the survey data, the control effect of each treatment group is calculated. The calculation is performed according to the following formula, and the calculation results are retained to two decimal places. The results are shown in Tables 1 to 3.

[0114] Insect population reduction rate (%) = (number of insects in the treatment group before treatment - number of live insects in the treatment group after treatment) / number of insects in the treatment group before treatment × 100;

[0115] Control effect (%) = (insect population reduction rate of the treatment group - insect population reduction rate of the control group) / (100 - insect population reduction rate of the control group) × 100.

[0116] Table 1 Results of the indoor acaricidal activity test on Tetranychus cinnabarinus

[0117]

[0118]

[0119]

[0120] Table 2 Results of indoor acaricidal activity test on citrus red spider

[0121]

[0122]

[0123]

[0124] Table 3 Results of the indoor acaricidal activity test on Tetranychus urticae

[0125]

[0126]

[0127]

[0128] The results show that the plant oil acaricide prepared with coriander oil, sandalwood oil, perilla seed oil, shea butter and castor oil can effectively kill adult cinnabarinus spider mites, adult citrus red spider mites and adult two-spotted spider mites. The acaricidal effect is close to that of ethidium bromide and significantly higher than that of mineral oil.

[0129] Test Example 2

[0130] Indoor egg killing experiment of Tetranychus cinnabarinus, Panonychus citri, and Tetranychus urticae:

[0131] 1. Preparation of test reagents:

[0132] The vegetable oil acaricides of Examples 1 to 5 were diluted 1000 times with clean water respectively.

[0133] Mineral oil ("Green") was diluted 1600 times with clean water and used as the mineral oil control group.

[0134] Spirodiclofen ("Mite Danger") was diluted 2000 times or 1500 times with clean water as the spirodiclofen control group.

[0135] Clear water served as the blank control group.

[0136] 2. Experimental methods:

[0137] (1) Preparation of leaf discs containing eggs: 20 adult female mites were transferred to a leaf disc with a diameter of 2.0 cm (with a soaked filter paper at the bottom), and then covered with a petri dish to keep moisture. The adult mites were removed within 36 hours and the leaf discs containing eggs were counted under a microscope.

[0138] (2) Investigation of the number of egg openings: The number of egg openings on each leaf disc was investigated under a microscope before immersion, with two replicates for each treatment.

[0139] (3) Immersion treatment: Immerse the leaf discs carrying mite eggs in the test agent for 10 seconds, take them out and keep them moisturized. Each treatment should be repeated at least twice.

[0140] (4) Cultivation and observation: The treated mite eggs and leaf discs were cultured under normal conditions. 5 to 7 days after drug application, the hatching of the mite eggs was investigated.

[0141] Note: After treatment, pay attention to the control of the temperature and humidity conditions in the constant temperature and humidity incubator to avoid excessive temperature differences, which may cause condensation to form and drip in the dish, resulting in abnormal death of eggs due to being soaked in water; it is also necessary to ensure that the environment has sufficient strong light, but not directly on the leaves.

[0142] (5) Result investigation: Regularly replenish water and moisturize the test materials of each treatment group and observe the hatching of eggs. On the 7th day after drug administration, record the number of hatched eggs of each treatment and record the investigation results in the original record book.

[0143] Survey indicators: Survey and record the number of hatched eggs in each treatment; take photos to record whether the leaf discs are damaged by pesticides; record the development status of the test mite eggs and the behavior of the nymphs, such as abnormal phenomena such as delayed or stopped development of the mite eggs, difficulty in hatching the nymphs, or painful struggles after hatching;

[0144] Based on the survey data, the prevention effect was calculated using the following formula. The calculation results were retained to two decimal places. The results are shown in Tables 4 to 6.

[0145] Egg hatching rate (%) = number of hatched eggs / total number of treated eggs × 100;

[0146] Control effect (%) = (hatching rate of eggs in the control area - hatching rate of eggs in the treatment area) / (hatching rate of eggs in the control area) × 100.

[0147] Table 4 Results of the indoor experiment on killing mites eggs of Tetranychus cinnabarinus

[0148]

[0149]

[0150] Table 5 Results of the indoor experiment on killing mites eggs of citrus red spider

[0151]

[0152] Table 6 Results of the indoor experiment on killing eggs of Tetranychus urticae

[0153]

[0154]

[0155] The results show that the plant oil acaricide prepared with coriander oil, sandalwood oil, perilla seed oil, shea butter and castor oil can effectively kill the eggs of cinnabarin spider mite, citrus red spider mite and two-spotted spider mite, and the killing effect is close to that of spirodiclofen and mineral oil.

[0156] Test Example 3

[0157] Experiment on killing adult mites and eggs by different dosage forms:

[0158] 1. Preparation of test reagents:

[0159] The vegetable oil acaricides of Examples 6 to 8 were diluted 400 times and 800 times with clean water, respectively.

[0160] 2. The killing experiments of the agents on adult mites and eggs were carried out according to the methods of Test Examples 1 and 2 respectively. The results are shown in Table 7.

[0161] Table 7 Experimental results of different dosage forms

[0162]

[0163] The results show that all three formulations have good control effects, but the control effects of microemulsion and water-emulsion on adult mites and eggs are reduced to varying degrees compared with emulsion, and emulsion has the best effect.

[0164] Test Example 4

[0165] Field experiment on Panonychus citri

[0166] The experiment was carried out in Jinma Village, Pujiang County on August 20, 2024. One citrus tree was treated each time. Five marks were randomly made on each citrus tree before the drug was applied. The number of adult mites and young mites on each mark was counted, and each mark was about 40 to 50. The front and back of the leaves of each citrus tree were evenly sprayed until small water droplets were formed. No other insecticides and fungicides were applied during the experiment. Data surveys were conducted 1, 3, and 7 days after the drug was applied to count the number of live insects remaining on the leaves. The formula for calculating the efficacy is as follows:

[0167] Pest reduction rate (%) = [(the number of insects in the treatment area before the drug application - the number of insects in the treatment area after the drug application) / the number of insects in the treatment area before the drug application] × 100;

[0168] Control effect (%) = (insect population reduction rate in treatment area - insect population reduction rate in control area) / (1 - insect population reduction rate in control area) × 100.

[0169] Table 8 Field test results

[0170]

[0171]

[0172] As can be seen from Table 8, the control effect of Example 6 diluted 100 times after use is equivalent to that of mineral oil diluted 200 times, but the control effect decreases significantly after dilution to 200 times, which is more than 10% lower than that of 100 times after use. The effective concentration of the vegetable oil agent or the dilution multiple can be increased to improve the control effect.

[0173] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A vegetable oil acaricide, characterized in that: The invention comprises the following raw materials by weight: 0.1 to 80 parts of vegetable oil, 1 to 30 parts of emulsifier and 1 to 99 parts of solvent; the vegetable oil is selected from any one or more of coriander oil, sandalwood oil, perilla seed oil, shea butter and castor oil.

2. The vegetable oil acaricide according to claim 1, characterized in that: The emulsifier includes any one or more of polyoxyethylene polyoxypropylene block copolymer, fatty alcohol polyoxyethylene ether, polyoxyethylene alkyl ester, alkylphenol polyoxyethylene ether, sorbitol polyoxyethylene ether, anhydrous sorbitan polyoxyethylene ether, alkylphenol formaldehyde condensate polyoxyethylene ether, sorbitan monooleate, fatty alcohol and ethylene oxide condensate, alkylbenzene sulfonate, fatty alcohol polyoxyethylene ether sodium sulfosuccinate, castor oil polyoxyethylene ether, lignin sulfonate, naphthalene and alkylnaphthalene sulfonate formaldehyde condensate, carboxymethyl cellulose, polycarboxylate dispersant, sodium tripolyphosphate, alkylnaphthalene sulfonic acid condensate sodium salt, calcium dodecylbenzene sulfonate and tristyrylphenol polyoxyethylene ether.

3. The vegetable oil acaricide according to claim 1, characterized in that: The solvent includes an organic solvent and / or an inorganic solvent; the organic solvent includes any one or more of methyl oleate, xylene, cyclohexanone, solvent oil 150#, solvent oil 200# and methylated soybean oil; and the inorganic solvent is water.

4. The vegetable oil acaricide according to claim 1, characterized in that: The vegetable oil acaricide further comprises 1 to 99 parts of a co-surfactant and 1 to 20 parts of an antifreeze agent.

5. The method for preparing the vegetable oil acaricide according to any one of claims 1 to 4, characterized in that: The following steps are involved: The raw material components are mixed according to proportion to prepare the vegetable oil acaricide.

6. Use of the vegetable oil acaricide according to any one of claims 1 to 4 in the preparation of products for controlling mites.

7. A method for controlling mites in the field, characterized in that: The method comprises the following steps: spraying the vegetable oil acaricide according to any one of claims 1 to 4 on crops.

8. The method according to claim 7, characterized in that The vegetable oil acaricide is diluted 1 to 2000 times.

9. The use according to claim 6 or the method according to any one of claims 7 to 8, characterized in that: The mites include spider mites, citrus spider mites and two-spotted spider mites.

10. The use according to claim 6 or the method according to any one of claims 7 to 8, characterized in that: The vegetable oil miticide kills adult mites, nymph mites, young mites and mite eggs.

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