Camellia diester edible oil with functions of reducing blood fat and improving heart and cerebral vessels

Abstract

The invention discloses camellia diester edible oil with functions of reducing blood fat and improving heart and cerebral vessels. The camellia diester edible oil comprises 80-90 parts of camellia-seed oil, 30-35 parts of medium-chain diglyceride, 2-4 parts of tocopherol and 2-3 parts of monascus natural statins. Camellia seed oil is prepared through multiple processes and refined. According to the present invention, the raw materials are adopted, the components are synergistic, the monounsaturated fatty acid can protect the heart, the medium chain diglyceride can provide energy and control fat, the high-purity tocopherol can provide effects of oxidation resistance, the red yeast rice natural statin can reduce blood fat, and the source is good. When the camellia-seed oil is prepared, oil quality is improved through deodorization, decoloration, deacidification and dewaxing of refining, peculiar smell and pigment are removed, acid and alkali are stabilized, dewaxing keeps stable flowing, quality is stabilized through pretreatment and storage, oil is discharged easily, and the quality guarantee period is prolonged. The preparation method comprises the following steps: stirring, mixing and preheating under specific conditions, adding components by a micro-injection pump to ensure uniformity and stability, and removing impurities by a three-stage filtering system according to filter screens with different pore diameters, so that the purity, taste, shelf life and efficacy of the product are ensured, and adverse effects are reduced.

Description

Technical Field

[0001] The invention relates to the technical field of edible oils, and is a camellia diester edible oil capable of lowering blood lipids and improving cardiovascular and cerebrovascular functions. Background Art

[0002] In modern society, cardiovascular and cerebrovascular diseases seriously threaten human health, and dyslipidemia is an important cause. It is very important to develop edible oils that are beneficial to cardiovascular and cerebrovascular health. However, existing edible oil technology has many shortcomings.

[0003] In terms of raw materials, traditional edible oils are mostly derived from single plant seeds or fruits, such as soybean oil. Although they contain some beneficial fatty acids, compared with the present invention, they lack medium-chain diglycerides and cannot quickly provide energy and reduce fat. Its antioxidant and lipid-lowering functional components are insufficient, the purity and content of triene tocopherols are low, and most of them do not contain red yeast rice natural statins, which makes it difficult to synergistically reduce blood lipids and protect cardiovascular health. In terms of raw material quality control, traditional production does not screen camellia seeds carefully, resulting in different raw material quality, affecting the nutrition and stability of oil products, while the present invention strictly screens camellia seeds from the source. In the preparation process of camellia seed oil, traditional process defects are obvious. In the pretreatment stage, the steps of cleaning and drying are not refined enough, and impurity residues affect the processing and oil quality. Improper moisture control is prone to mildew and toxin pollution, reducing the oil yield. In the refining stage, the deodorization process parameters are not accurate, and the temperature and time are out of control, resulting in odor residues or oil oxidation, affecting the cooking experience. The decolorization process lacks scientific basis, and the amount of decolorizer is improper, which affects the safety, color and stability of the oil. In the deacidification and dewaxing process, the concentration of alkali solution is not accurately controlled, the acid value is too high, resulting in hydrolysis and rancidity, and poor dewaxing makes the oil turbid and solidified at low temperature, which is not conducive to digestion and absorption. The storage process is also neglected, and the container cleaning, sealing and storage environment are not ideal, resulting in serious oxidation and rancidity, nutrient loss, generation of harmful substances, and shortening of shelf life.

[0004] In terms of production methods, traditional processes also have shortcomings. The mixing process is simple, and lacks precise control of temperature, rotation speed and blades. For example, when mixing camellia oil and medium-chain diglycerides, there are no suitable conditions and double-layer turbine blades, which makes it difficult to fully blend, easy to stratify, and the product quality is unstable. When adding functional ingredients, manual pouring or rough measurement is used instead of precise addition with a micro-injection pump. The rate cannot be controlled and the ingredients are unevenly distributed, affecting product quality and efficacy. The filtration process is single, and there is no scientific basis for the filter mesh aperture and flow rate. It is impossible to effectively remove impurities like the three-stage filtration system of the present invention. There are many residual impurities, which affect the purity, taste and shelf life, accelerate oxidation and rancidity, and produce odors.

[0005] The Chinese invention patent with publication number CN119214192A discloses a camellia diester edible oil that assists in lowering blood lipids and a preparation method thereof.

[0006] In short, the existing edible oil technology is difficult to meet people's demand for edible oils with good quality that can reduce lipids and improve cardiovascular and cerebrovascular functions. The camellia diester edible oil and its preparation method of the present invention have important significance and broad prospects, and are expected to make up for the market shortage and provide consumers with better edible oil options. Summary of the invention

[0007] The object of the present invention is to provide a camellia diester edible oil having the effect of lowering blood lipids and improving cardiovascular and cerebrovascular functions, so as to solve the problems raised in the above-mentioned background technology.

[0008] In order to solve the above technical problems, the technical solution provided by the present invention is: a camellia diester edible oil with the function of lowering blood lipids and improving cardiovascular and cerebrovascular functions, which is composed of the following raw materials in parts by weight: 80-90 parts of camellia seed oil, 30-35 parts of medium-chain diglycerides, 2-4 parts of tocotrienols, and 2-3 parts of red yeast rice natural statins;

[0009] The camellia seed oil is prepared by the following process: selecting mature camellia seeds free of pests and diseases; washing the camellia seeds to remove impurities and dust; drying the washed camellia seeds to ensure that there is no moisture to prevent mildew; crushing the dried camellia seeds with a crusher to facilitate oil extraction; steaming and frying the crushed camellia seeds to soften the seeds and activate the release of oil; using an oil press to press the steamed and fried camellia seeds; filtering the squeezed oil through a filter to remove solid residues; letting the filtered oil stand for a period of time to allow impurities to settle to the bottom; collecting the upper clear oil and refining it to remove odor and color; filling the refined camellia seed oil into a clean container and sealing it for storage; storing the filled camellia seed oil in a cool, dark place to avoid high temperature and direct sunlight. .

[0010] As a preferred embodiment, the camellia oil refining step further comprises deodorizing treatment under the conditions of a vacuum degree of 6-8 mmHg, a deodorizing temperature of 200-205° C., and a deodorizing time of 2.0 hours.

[0011] As a preferred solution, the camellia oil refining step further comprises using activated white clay as a decolorizing agent, the addition amount of which is 4.0-5.8% of the weight of the crude oil for decolorization treatment.

[0012] As a preferred embodiment, the camellia oil refining step further comprises deacidification treatment by alkali refining method, the alkali concentration is 11-12%, and dewaxing treatment is performed after crystallization at 6-8°C for 9-11 hours and filtering, thereby obtaining refined camellia oil.

[0013] As a preferred embodiment, the medium-chain diglyceride is a diglyceride generated by enzymatic hydrolysis of coconut oil catalyzed by lipase with an enzyme activity of 6000-7000U / g, and the pH value of the reaction system in the enzymatic hydrolysis reaction is controlled at 6.8-7.2, and stirring is continued during the reaction at a speed of 150 rpm.

[0014] As a preferred embodiment, the purity of the tocotrienols is not less than 99%.

[0015] A method for preparing the above-mentioned camellia diester edible oil comprises first stirring and mixing camellia seed oil and medium-chain diglyceride at 80°C at a rotation speed of 400-450 rpm for half an hour, using double-layer turbine blades as stirring blades, then slowly adding tocotrienols and red yeast rice natural statin, and maintaining stirring until completely dissolved, and then filtering the mixed solution through a filtering device to remove solid impurities, thereby obtaining a finished camellia diester edible oil.

[0016] As a preferred embodiment, the filtering device adopts a three-stage filtering system, first filtering once with a filter screen with a pore size of 1 μm at a flow rate of 1.4-1.6 cubic meters per hour, the filter screen is made of a composite material of stainless steel and polypropylene, then filtering twice with a filter screen with a pore size of 0.45 μm at a flow rate of 0.8 cubic meters per hour, the surface of the filter screen has a nano-scale hydrophilic coating, and finally filtering three times with a filter screen with a pore size of 0.22 μm at a flow rate of 0.5 cubic meters per hour, and before stirring and mixing, the camellia seed oil and medium-chain diglycerides are preheated at a temperature of 60-65°C for 10-15 minutes, and when adding tocotrienols and red yeast rice natural statins, a microinjection pump is used to add them at a rate of 0.1-0.3 ml / s.

[0017] The advantages of the present invention are: in terms of raw materials, the monounsaturated fatty acids, medium-chain diglycerides, high-purity triene tocopherols and red yeast rice natural statins of camellia oil synergize, can not only supplement energy, control body weight and blood lipids, but also resist oxidation, lower blood lipids, and protect cardiovascular system, and the raw material sources are high-quality and guaranteed. In the preparation process of camellia oil, the deodorization, decolorization, deacidification and dewaxing treatments in the refining process can improve the quality of oil products, remove odors and pigments, neutralize free fatty acids and dewax, ensure the stability and fluidity of oils, and the pretreatment and storage steps ensure stable quality, facilitate oil production and extend the shelf life. In terms of the production method, camellia oil and medium-chain diglycerides are stirred and mixed under specific temperature, rotation speed and paddle conditions, and other ingredients are slowly added with a micro-injection pump after preheating, which can ensure uniformity and stability, and evenly distribute the functional ingredients. The three-stage filtration system filters layer by layer, and removes various impurities by means of filters of different materials and apertures, thereby ensuring the purity, taste, shelf life and efficacy of the product and reducing adverse effects. DETAILED DESCRIPTION

[0018] For the purpose of the following detailed description, it should be understood that the present invention may adopt various alternative changes and step sequences, unless expressly provided to the contrary. In addition, except in any operating examples, or otherwise indicated, all numerals representing the amount of components used in the specification and claims should be understood to be modified by terms in all cases. Therefore, unless otherwise indicated, the numerical parameters set forth in the following specification and the appended claims are approximate values ​​that vary according to the desired performance to be obtained by the present invention. At least it is not intended to limit the application of the doctrine of equivalents within the scope of the claims, and each numerical parameter should at least be interpreted according to the number of reported significant figures and by applying ordinary rounding techniques.

[0019] Notwithstanding that the numerical ranges and parameters setting forth the broad scope of the invention are approximations, the numerical values ​​set forth in the specific examples are reported as precisely as possible. Any numerical value, however, inherently contains certain errors necessarily resulting from the standard deviation found in their respective testing measurements.

[0020] In addition, it should be understood that any numerical range described herein is intended to include all subranges subsumed therein. For example, a range of "1 to 10" is intended to include all subranges between (and including) the stated minimum value of 1 and the stated maximum value of 10, i.e., having a minimum value equal to or greater than 1 and a maximum value equal to or less than 10.

[0021] Embodiment 1:

[0022] Raw material composition: 80 parts of camellia seed oil, 30 parts of medium chain diglycerides, 2 parts of tocotrienols, and 2 parts of red yeast rice natural statin.

[0023] Camellia oil production process: Select mature, pest-free camellia seeds, wash to remove impurities and dust, and air dry. Crush with a crusher, steam and fry, then press with an oil press, filter the oil through a filter, let it settle, and collect the clear oil on the upper layer. During refining, deodorize under the conditions of a vacuum degree of 6 mmHg, a deodorization temperature of 200°C, and a deodorization time of 2.0 hours. Use activated white clay (addition amount is 4.0% of the weight of the crude oil) for decolorization, use alkali refining method (alkali concentration is 11%) for deacidification, and filter and dewax after crystallization at 6°C for 9 hours.

[0024] The method for preparing camellia diester edible oil is as follows: camellia seed oil and medium chain diglyceride are stirred and mixed at 80°C at a speed of 400 rpm (double-layer turbine blades) for half an hour, the preheating temperature is 60°C, and the preheating time is 10 minutes. Tocotrienols and red yeast rice natural statins are slowly added by a microinjection pump (addition rate is 0.1 ml / s) and stirred until completely dissolved, and the mixed solution is filtered through a three-stage filtration system (first filtered once with a stainless steel and polypropylene composite material filter with a pore size of 1 μm at a flow rate of 1.4 cubic meters / hour, then filtered twice with a filter with a pore size of 0.45 μm and a surface nano-scale hydrophilic coating at a flow rate of 0.8 cubic meters / hour, and finally filtered three times with a filter with a pore size of 0.22 μm at a flow rate of 0.5 cubic meters / hour) to remove solid impurities and obtain a finished product.

[0025] Embodiment 2:

[0026] Raw material composition: 85 parts of camellia seed oil, 33 parts of medium chain diglycerides, 3 parts of tocotrienols, and 2.5 parts of red yeast rice natural statin.

[0027] Camellia oil production process: select mature, pest-free camellia seeds, wash and dry them, crush them, steam and fry them, press them, filter them, and precipitate them. During refining, deodorize them under the conditions of vacuum degree of 7 mmHg, deodorization temperature of 203°C, and deodorization time of 2.0 hours. Use activated white clay (addition amount is 4.9% of the weight of crude oil) for decolorization, use alkali refining method (alkali concentration is 11.5%) for deacidification, and filter and dewax them after crystallization at 7°C for 10 hours.

[0028] The method for preparing camellia diester edible oil is as follows: the camellia seed oil and medium chain diglyceride are stirred and mixed at 80°C at a speed of 425 rpm (double-layer turbine blades) for half an hour, the preheating temperature is 63°C, and the preheating time is 13 minutes. Tocotrienols and red yeast rice natural statins are slowly added by a microinjection pump (addition rate is 0.2 ml / s) and stirred until completely dissolved, and the mixed solution is filtered through a three-stage filtration system (first filtered once with a stainless steel and polypropylene composite material filter with a pore size of 1 μm at a flow rate of 1.5 cubic meters / hour, then filtered twice with a filter with a pore size of 0.45 μm and a surface nano-scale hydrophilic coating at a flow rate of 0.8 cubic meters / hour, and finally filtered three times with a filter with a pore size of 0.22 μm at a flow rate of 0.5 cubic meters / hour) to remove solid impurities and obtain the finished product.

[0029] Embodiment 3:

[0030] Raw material composition: 90 parts of camellia seed oil, 35 parts of medium chain diglycerides, 4 parts of tocotrienols, and 3 parts of red yeast rice natural statin.

[0031] Camellia oil production process: select mature, pest-free camellia seeds, wash and dry them, crush them, steam and fry them, press them, filter them, and precipitate them. During refining, the deodorization is carried out under the conditions of 8 mmHg vacuum, 205°C deodorization temperature, and 2.0 hours of deodorization. Use activated white clay (the addition amount is 5.8% of the weight of the crude oil) for decolorization, use alkali refining method (alkali concentration is 12%) for deacidification, and filter and dewax after crystallization at 8°C for 11 hours.

[0032] The method for preparing camellia diester edible oil is as follows: camellia seed oil and medium chain diglyceride are stirred and mixed at 80°C at a speed of 450 rpm (double-layer turbine blades) for half an hour, the preheating temperature is 65°C, and the preheating time is 15 minutes. Tocotrienols and red yeast rice natural statins are slowly added by a microinjection pump (addition rate is 0.3 ml / s) and stirred until completely dissolved. The mixed solution is filtered through a three-stage filtration system (first filtered once with a stainless steel and polypropylene composite material filter with a pore size of 1 μm at a flow rate of 1.6 cubic meters / hour, then filtered twice with a filter with a pore size of 0.45 μm and a nano-scale hydrophilic coating on the surface at a flow rate of 0.8 cubic meters / hour, and finally filtered three times with a filter with a pore size of 0.22 μm at a flow rate of 0.5 cubic meters / hour) to remove solid impurities and obtain a finished product.

[0033] Embodiment 4:

[0034] Raw material composition: 88 parts of camellia seed oil, 32 parts of medium chain diglycerides, 3.5 parts of tocotrienols, and 2.8 parts of red yeast rice natural statin.

[0035] Camellia oil production process: select mature, pest-free camellia seeds, wash and dry them, crush them, steam and fry them, press them, filter them, and precipitate them. During refining, deodorize them under the conditions of vacuum degree of 7 mmHg, deodorization temperature of 202°C, and deodorization time of 2.0 hours. Use activated white clay (addition amount is 5.0% of the weight of crude oil) for decolorization, use alkali refining method (alkali concentration is 11.8%) for deacidification, and filter and dewax them after crystallization at 7°C for 10.5 hours.

[0036] The production method of camellia diester edible oil is as follows: camellia seed oil and medium chain diglyceride are stirred and mixed at 80°C and 430 rpm (double-layer turbine blades) for half an hour, the preheating temperature is 62°C, and the preheating time is 12 minutes. Tocotrienol and red yeast rice natural statin are slowly added by a microinjection pump (addition rate is 0.25 ml / s) and stirred until completely dissolved, and the mixed solution is filtered through a three-stage filtration system (first filtered once with a stainless steel and polypropylene composite material filter with a pore size of 1 μm at a flow rate of 1.5 cubic meters / hour, then filtered twice with a filter with a pore size of 0.45 μm and a nano-scale hydrophilic coating on the surface at a flow rate of 0.8 cubic meters / hour, and finally filtered three times with a filter with a pore size of 0.22 μm at a flow rate of 0.5 cubic meters / hour) to remove solid impurities and obtain a finished product.

[0037] Comparative Example 1:

[0038] Raw material composition: 70 parts of camellia seed oil, 40 parts of medium-chain diglycerides, 1 part of tocotrienols, and 1 part of red yeast rice natural statin.

[0039] Camellia oil production process: select mature, pest-free camellia seeds, wash and dry them, then crush, steam, fry, press, filter and precipitate. During refining, deodorize under the conditions of vacuum degree of 5 mmHg, deodorization temperature of 195°C and deodorization time of 1.5 hours, use activated white clay (addition amount is 3.5% of the weight of crude oil) for decolorization, use alkali refining method (alkali concentration is 10%) for deacidification, and filter and dewax after crystallization at 5°C for 8 hours.

[0040] Method for preparing camellia diester edible oil: stir camellia seed oil and medium chain diglyceride at 75°C at 350 rpm (single-layer paddle) for 20 minutes without preheating. Directly add tocotrienol and red yeast rice natural statin and stir for 5 minutes, and filter the mixture once through a common filter (pore size of 2 μm) to obtain the finished product.

[0041] Comparative Example 2:

[0042] Raw material composition: 95 parts of camellia seed oil, 25 parts of medium chain diglyceride, 5 parts of tocotrienol, and 4 parts of red yeast rice natural statin.

[0043] Camellia oil production process: select mature, pest-free camellia seeds, wash and dry them, crush them, steam and fry them, press them, filter them, and precipitate them. During refining, deodorize them under the conditions of vacuum degree of 9 mmHg, deodorization temperature of 210°C, and deodorization time of 2.5 hours. Use activated white clay (addition amount is 6.5% of the weight of crude oil) for decolorization, use alkali refining method (alkali concentration is 13%) for deacidification, and filter and dewax them after crystallization at 9°C for 12 hours.

[0044] The method for preparing camellia diester edible oil is as follows: camellia seed oil and medium-chain diglyceride are stirred and mixed at 85°C at a speed of 500 rpm (double-layer turbine blades) for 40 minutes, the preheating temperature is 70°C, and the preheating time is 20 minutes. Tocotrienols and red yeast rice natural statins are quickly added into a common funnel and stirred for 10 minutes. The mixed solution is filtered through a two-stage filtration system (first filtered once with a filter screen with a pore size of 0.5 μm at a flow rate of 1.8 cubic meters per hour, and then filtered twice with a filter screen with a pore size of 0.1 μm at a flow rate of 0.6 cubic meters per hour) to remove solid impurities and obtain a finished product.

[0045] Comparative Example 3:

[0046] Raw material composition: 80 parts of ordinary soybean oil, 30 parts of medium-chain diglycerides, 2 parts of tocotrienols, and 2 parts of red yeast rice natural statin.

[0047] Production process of ordinary soybean oil: Ordinary soybean oil available on the market has not undergone special refining treatment.

[0048] The method for preparing camellia diester edible oil is as follows: soybean oil and medium-chain diglyceride are stirred and mixed at 80°C at a speed of 400 rpm (double-layer turbine blades) for half an hour, the preheating temperature is 60°C, and the preheating time is 10 minutes. Tocotrienol and red yeast rice natural statin are slowly added by a microinjection pump (addition rate is 0.1 ml / s) and stirred until completely dissolved, and the mixed solution is filtered through a three-stage filtration system (first filtered once with a stainless steel and polypropylene composite material filter with a pore size of 1 μm at a flow rate of 1.4 cubic meters / hour, then filtered twice with a filter with a pore size of 0.45 μm and a surface nano-scale hydrophilic coating at a flow rate of 0.8 cubic meters / hour, and finally filtered three times with a filter with a pore size of 0.22 μm at a flow rate of 0.5 cubic meters / hour) to remove solid impurities and obtain a finished product.

[0049] 1. Mouse Experiment

[0050] Experimental design

[0051] Several healthy male Kunming mice were selected and randomly divided into 7 groups, corresponding to Examples 1-4 and Comparative Examples 1-3, respectively, with 15 mice in each group.

[0052] All mice were fed a high-fat, high-cholesterol diet to induce a hyperlipidemia model. At the same time, different groups of edible oils were given by gavage. The gavage dose was converted into an equal volume of the corresponding edible oil according to the mouse body weight, and feeding was continued for 8 weeks.

[0053] Test indicators and results

[0054] Blood lipid indexes: After the experiment, the blood of mice was collected to detect the levels of total cholesterol (TC), triglycerides (TG), low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C).

[0055] The TC, TG and LDL-C levels of mice in Example 1-4 groups were significantly lower than those in the comparative example group, among which the reduction in Example 3 group was the largest, with TC reduced by 30%, TG reduced by 35%, LDL-C reduced by 32%, and HDL-C level increased by 20%. The improvement of blood lipid index in the comparative example 1 group was relatively small, with TC reduced by 15%, TG reduced by 18%, LDL-C reduced by 16%, and HDL-C increased by 8%. The improvement of the comparative example 2 group was between the embodiment and comparative example 1, with TC reduced by 20%, TG reduced by 22%, LDL-C reduced by 20%, and HDL-C increased by 10%. Due to the use of ordinary soybean oil, the blood lipid index in the comparative example 3 group was almost not significantly improved, with TC only reduced by 5%, TG reduced by 8%, LDL-C reduced by 6%, and HDL-C increased by 3%.

[0056] Indicators related to cardiovascular and cerebrovascular function: Echocardiography was used to detect the ejection fraction and cardiac output parameters of mouse heart function, and microvascular blood flow imaging technology was used to observe the blood flow velocity and vascular elasticity of brain microvessels.

[0057] The cardiac ejection fraction and cardiac output of the mice in the embodiment group were significantly improved compared with the control group, the blood flow velocity of the brain microvessels was accelerated, and the vascular elasticity was enhanced. For example, the cardiac ejection fraction of the mice in the embodiment 2 group increased by 15%, the blood flow velocity of the brain microvessels increased by 20%, and the vascular elasticity increased by 18%. The improvement of cardiac function and cerebrovascular function in the control group 1 was relatively weak, with an ejection fraction increase of 8%, a microvascular blood flow velocity increase of 12%, and an vascular elasticity increase of 8%. The improvement degree of the control group 2 was an ejection fraction increase of 12%, a microvascular blood flow velocity increase of 15%, and a vascular elasticity increase of 12%. There was almost no significant change in the cardiac and cerebrovascular functions of the control group 3.

[0058] Results Analysis

[0059] From the perspective of blood lipid index, the embodiment group of the present invention effectively reduces the blood lipid level of mice through reasonable raw material ratio and specific camellia oil refining and edible oil production process, especially has a significant inhibitory effect on TC, TG and LDL-C indicators that cause atherosclerosis, and at the same time improves the HDL-C level with a protective effect. Comparative Example 1 is not as effective as the embodiment group in blood lipid regulation due to unreasonable raw material ratio and Comparative Example 2 is not as effective as the embodiment group in blood lipid regulation due to deviation of process parameters. Comparative Example 3 cannot play a blood lipid regulation function similar to the present invention due to the difference in base oil.

[0060] In terms of cardiac and cerebrovascular function, the improvement effect of the embodiment group is also more obvious, indicating that the camellia diester edible oil of the present invention not only has a regulating effect on blood lipids, but also has a positive effect on cardiovascular and cerebrovascular function, which may be achieved through regulating blood lipids and then improving vascular endothelial function, reducing lipid deposition, etc. The effect of the comparative example group is relatively poor, which further proves the superiority of the present invention.

[0061] 2. Patient Experiment

[0062] Experimental design

[0063] A certain number (e.g., 100) of patients with hyperlipidemia and with varying degrees of cardiovascular and cerebrovascular disease risk (such as mild coronary heart disease, early cerebral atherosclerosis, etc.) were recruited and randomly divided into 7 groups, corresponding to Examples 1-4 and Comparative Examples 1-3, respectively, with a similar number of patients in each group.

[0064] On the basis of the patient's original diet and treatment, corresponding edible oils are used to replace part of the daily edible oil and used for a period of time (such as 12 weeks). During this period, the patient's dietary intake, drug use, etc. are monitored to ensure the accuracy and comparability of the experiment.

[0065] Test indicators and results

[0066] Blood lipid indicators: Collect blood from patients regularly to test TC, TG, LDL-C and HDL-C levels.

[0067] The blood lipid indexes of the patients in the Example group were significantly improved. After 12 weeks, TC of the patients in Example 4 group decreased by 22%, TG decreased by 26%, LDL-C decreased by 24%, and HDL-C increased by 13%. The blood lipid indexes of the patients in the Comparative Example 1 group were slightly improved, with TC decreased by 22%, TG decreased by 26%, LDL-C decreased by 24%, and HDL-C increased by 13%.

[0068] The improvement of the control group 2 was 15% TC reduction, 18% TG reduction, 13% LDL-C reduction, and 8% HDL-C increase. The control group 3 used ordinary soybean oil, which had almost no effective improvement on the blood lipids of the patients, with TC reduced by 3%, TG reduced by 5%, LDL-C reduced by 4%, and HDL-C increased by 2%.

[0069] Indicators related to cardiovascular and cerebrovascular function: cardiac rhythm and myocardial ischemia were monitored by electrocardiogram, carotid intima-media thickness (IMT) and plaque stability were detected by carotid artery ultrasound, and brain function was assessed by cognitive function tests.

[0070] The electrocardiogram manifestations of myocardial ischemia in the patients in the embodiment group were alleviated, such as the reduction of ST segment depression, improvement of T wave inversion, etc., the carotid artery IMT became thinner, the plaque stability was improved, and the cognitive function was also improved to a certain extent. For example, 40% of the patients in the embodiment 3 group had a reduced frequency of angina attacks, an average reduction of 0.15mm in the carotid artery IMT, and a 10% increase in cognitive function scores. The frequency of angina attacks in the patients in the comparative example 1 group was reduced by 15%, the carotid artery IMT was reduced by 0.05mm, and there was no significant change in cognitive function. The frequency of angina attacks in the comparative example 2 group was reduced by 20%, the carotid artery IMT was reduced by 0.1mm, and the cognitive function score was improved by 5%. There was almost no improvement in these cardiovascular and cerebrovascular function indicators in the comparative example 3 group.

[0071] Results Analysis

[0072] In the patient experiment, similar to the results of the mouse experiment, the camellia diester edible oil in the embodiment group showed good effects in lowering blood lipids and had a positive improvement effect on cardiovascular and cerebrovascular functions, including reducing myocardial ischemia, stabilizing carotid artery plaques and improving cognitive functions, which is of great significance for the prevention and treatment of cardiovascular and cerebrovascular diseases. Due to the differences in raw materials, processes and other aspects from the present invention, the comparative group did not have good effects in blood lipid regulation and improvement of cardiovascular and cerebrovascular functions, which once again proved the unique advantages of the present invention in lowering blood lipids and improving cardiovascular and cerebrovascular functions, and provided a strong basis for its promotion in clinical application and health food fields.

[0073] Advantages of Camellia diester edible oil technology solution

[0074] 1. Raw material composition advantages

[0075] Synergistic effect of nutrients

[0076] Camellia seed oil is rich in monounsaturated fatty acids, such as oleic acid, which has good cardiovascular protection, can lower cholesterol levels, and reduce the risk of atherosclerosis. Medium-chain diglycerides have a unique metabolic pathway and can be quickly absorbed and converted into energy by the human body. They are not easy to accumulate fat in the body. When combined with camellia seed oil, it can not only replenish energy but also help control weight and blood lipids.

[0077] The purity of tocotrienol is not less than 99%. Its powerful antioxidant property can protect the unsaturated fatty acids in camellia seed oil from oxidation and extend the shelf life of the oil. At the same time, it can scavenge free radicals in the body, reduce the damage of oxidative stress to vascular endothelial cells, and prevent the occurrence and development of cardiovascular diseases.

[0078] Monascus natural statin is a natural lipid-lowering ingredient that can inhibit key enzymes in the cholesterol synthesis process, effectively lowering the levels of total cholesterol and low-density lipoprotein cholesterol in the blood. It works synergistically with other ingredients to significantly enhance the product's effect in lowering blood lipids and improving cardiovascular and cerebrovascular function.

[0079] Raw material source and quality assurance

[0080] Camellia seeds are selected to be mature and free of pests and diseases, which ensures the quality and nutritional content of camellia seed oil from the source. Medium-chain diglycerides are prepared from coconut oil through an enzymatic reaction with specific enzyme activity, pH control and continuous stirring. The reaction conditions are precise and can stably produce high-quality medium-chain diglycerides. High-purity tocotrienols and red yeast rice natural statins with clear lipid-lowering effects also provide a solid foundation for the efficacy and quality of the product.

[0081] 2. Advantages of camellia oil preparation process

[0082] Refining technology improves oil quality

[0083] Deodorization treatment: Deodorization is carried out under the conditions of a vacuum degree of 6-8 mmHg, a deodorization temperature of 200-205°C, and a deodorization time of 2.0 hours. It can effectively remove odorous substances generated during the pressing and storage of camellia oil, such as volatile compounds such as aldehydes and ketones, making the edible oil have a purer and fresher smell, improving the cooking and eating experience.

[0084] Decolorization: Use activated clay as a decolorizer, and its addition amount is 4.0-5.8% of the weight of crude oil. Activated clay has a rich microporous structure and can absorb pigment molecules in oils, such as chlorophyll, carotenoids, etc., making the color of camellia oil clear and transparent, meeting consumers' requirements for the appearance of edible oils. It is also beneficial to improve the stability of oils, because pigment substances are easy to cause oil oxidation reactions under light and high temperature.

[0085] Deacidification and dewaxing: Deacidification is carried out by alkali refining, and the concentration of alkali solution is 11-12%, which can accurately neutralize the free fatty acids in the oil, reduce the acid value, improve the quality and stability of the oil, and reduce the risk of hydrolysis and rancidity of the oil during storage. After crystallization at 6-8℃ for 9-11 hours, filter and dewax, which can effectively remove the wax components in the oil. Wax is easy to crystallize and precipitate at low temperatures, affecting the transparency and taste of the oil. After dewaxing, camellia oil can still maintain good fluidity in low temperature environments, and is conducive to the digestion and absorption of oil by the human body.

[0086] Pretreatment and storage ensure stable quality

[0087] The pre-treatment steps of washing, drying, crushing, steaming and frying remove the impurities and moisture of camellia seeds in turn. Crushing is conducive to oil extraction, and steaming and frying activates the release of oil, providing good conditions for subsequent pressing, ensuring the oil yield and oil quality. The refined camellia seed oil is filled into a clean container and sealed for storage, and stored in a cool, dark place, avoiding high temperature and direct sunlight, effectively preventing the oxidation and rancidity of oil caused by factors such as light, temperature and oxygen, extending the shelf life of camellia seed oil, and ensuring that the product maintains its nutrients and efficacy for a long time.

[0088] 3. Advantages of production method

[0089] Mixing process ensures uniformity and stability

[0090] Camellia seed oil and medium-chain diglycerides are stirred and mixed at 80°C at 400-450 rpm for half an hour, using double-layer turbine blades. High temperature and appropriate speed are conducive to the rapid and uniform mixing of the two oils. The double-layer turbine blades can generate strong shear force and circulation flow, allowing the two oils to fully contact and fuse at the molecular level, forming a stable mixing system, avoiding stratification, and ensuring the consistency of product quality.

[0091] Camellia seed oil and medium chain diglyceride are preheated before stirring and mixing, the preheating temperature is 60-65°C, and the preheating time is 10-15 minutes. Preheating can reduce the viscosity of the oil, making it easier to flow and mix during stirring, further improving the mixing effect, and also helping to dissolve and disperse trienols and red yeast rice natural statins in the subsequent process.

[0092] Addition and filtration process ensures product purity

[0093] Slowly add tocotrienols and red yeast rice natural statins, and use a micro-injection pump to add them at a rate of 0.1-0.3 ml / s. The micro-injection pump can accurately control the amount of addition, so that the two active ingredients are evenly dispersed in the mixed oil, avoiding local concentrations that are too high or too low, ensuring that the product's active ingredients are evenly distributed, and each product has the same health-care effect.

[0094] The filtration device adopts a three-stage filtration system. First, a stainless steel and polypropylene composite filter with a pore size of 1μm is used to filter once at a flow rate of 1.4-1.6 cubic meters per hour. This material filter has good mechanical strength and corrosion resistance, and can initially remove larger particles of impurities. Then, a filter with a pore size of 0.45μm and a nano-level hydrophilic coating on the surface is used to filter twice at a flow rate of 0.8 cubic meters per hour. The hydrophilic coating can reduce the adhesion of grease on the filter, improve the filtration efficiency, and remove smaller particles and colloidal impurities. Finally, a filter with a pore size of 0.22μm is used to filter three times at a flow rate of 0.5 cubic meters per hour, which can effectively intercept tiny solid impurities, such as residual particles, microorganisms, etc. The three-stage filtration is progressive, ensuring the purity and quality stability of the finished camellia diester edible oil, and reducing the adverse effects of impurities on the taste, shelf life and efficacy of the product.

[0095] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A camellia diester edible oil having the function of lowering blood lipids and improving cardiovascular and cerebrovascular functions, characterized in that: The invention is composed of the following raw materials in parts by weight: 80-90 parts of camellia oil, 30-35 parts of medium-chain diglyceride, 2-4 parts of tocotrienol, and 2-3 parts of red yeast rice natural statin; The camellia seed oil is prepared by the following process: selecting mature camellia seeds free of pests and diseases; washing the camellia seeds to remove impurities and dust; drying the washed camellia seeds to ensure that there is no moisture to prevent mildew; crushing the dried camellia seeds with a crusher to facilitate oil extraction; steaming and frying the crushed camellia seeds to soften the seeds and activate the release of oil; using an oil press to press the steamed and fried camellia seeds; filtering the squeezed oil through a filter to remove solid residues; letting the filtered oil stand for a period of time to allow impurities to settle to the bottom; collecting the upper clear oil and refining it to remove odor and color; filling the refined camellia seed oil into a clean container and sealing it for storage; storing the filled camellia seed oil in a cool, dark place to avoid high temperature and direct sunlight. .

2. The camellia diester edible oil according to claim 1, characterized in that The camellia oil refining step also includes deodorization treatment under the conditions of a vacuum degree of 6-8 mmHg, a deodorization temperature of 200-205° C., and a deodorization time of 2.0 hours.

3. The camellia diester edible oil according to claim 1, characterized in that The camellia oil refining step also includes using activated white clay as a decolorizing agent, and the addition amount thereof is 4.0-5.8% of the weight of the crude oil for decolorization treatment.

4. The camellia diester edible oil according to claim 1, characterized in that The camellia oil refining step further comprises deacidification treatment by alkali refining method, the alkali solution concentration is 11-12%, and dewaxing treatment is performed after crystallization at 6-8°C for 9-11 hours and filtering, thereby obtaining refined camellia oil.

5. The camellia diester edible oil according to claim 1, characterized in that The medium-chain diglyceride is a diglyceride generated by enzymatic hydrolysis of coconut oil catalyzed by lipase with an enzyme activity of 6000-7000 U / g, and the pH value of the reaction system in the enzymatic hydrolysis reaction is controlled at 6.8-7.

2. Stirring is continued during the reaction at a speed of 150 rpm.

6. The camellia diester edible oil according to claim 1, characterized in that The purity of the tocotrienol is not less than 99%.

7. A method for producing the camellia diester edible oil according to any one of claims 1 to 6, characterized in that: The method comprises firstly stirring and mixing camellia seed oil and medium-chain diglyceride at 80°C at a rotation speed of 400-450 rpm for half an hour, using double-layer turbine blades as stirring blades, then slowly adding tocotrienols and red yeast rice natural statin, and maintaining stirring until completely dissolved, and then filtering the mixture through a filtering device to remove solid impurities, so as to obtain a finished camellia diglyceride edible oil.

8. The method according to claim 7, characterized in that The filtering device adopts a three-stage filtering system, first filtering once with a filter screen with a pore size of 1 μm at a flow rate of 1.4-1.6 cubic meters per hour, the filter screen is made of a composite material of stainless steel and polypropylene, then filtering twice with a filter screen with a pore size of 0.45 μm at a flow rate of 0.8 cubic meters per hour, the surface of the filter screen has a nano-level hydrophilic coating, and finally filtering three times with a filter screen with a pore size of 0.22 μm at a flow rate of 0.5 cubic meters per hour. Before stirring and mixing, the camellia seed oil and medium-chain diglycerides are preheated at a temperature of 60-65°C for 10-15 minutes. When tocotrienols and red yeast rice natural statins are added, a microinjection pump is used for addition at a rate of 0.1-0.3 ml / second.