Pet food with functions of improving immunity and resisting oxidation

Abstract

The invention provides a pet food with functions of improving immunity and resisting oxidation. The pet food is prepared from the following components in percentage by weight: 0.1 to 3 percent of lactobacillus paracasei metabiotics, 0.5 to 2.5 percent of folium cortex eucommiae extract and 0.5 to 4 percent of pumpkin seed superfine powder, the lactobacillus paracasei metaplasm is prepared by carrying out inactivation, concentration and freeze-drying on a lactobacillus paracasei ZYhyy-004 bacterial solution, and the lactobacillus paracasei metaplasm is prepared from the lactobacillus paracasei ZYhyy-004 bacterial solution. The addition amount of the lactobacillus paracasei metabiotics is 1 * 10 < 9 >-1 * 10 < 11 > CFU / kg pet food equivalent to the addition amount of the probiotics before inactivation. By reasonably selecting natural active ingredients and combining with an advanced preparation technology, the invention aims to effectively enhance the immunity of pets and improve the antioxidant level, thereby delaying senescence and enhancing health.

Description

Technical Field

[0001] The invention belongs to the technical field of pet food, and in particular relates to pet food with immunity-enhancing and anti-oxidation functions. Background Art

[0002] With the improvement of the status of pets in human families, the health of pets has received more and more attention. Pets are often attacked by various germs and viruses in daily life, and the strength of the immune system directly affects the growth and development of pets and their disease resistance. The reasons for the low immunity of many pets include long-term malnutrition, excessive environmental pressure, and genetic factors. The immunity and antioxidant capacity of pets directly affect their healthy lifespan, especially for elderly pets, whose immunity gradually declines and they are prone to various diseases. In order to enhance the immunity of pets, some related products have appeared on the market. However, most of the existing immune enhancement products have either single ingredients, limited effects, and may have side effects during use. Therefore, it is of great practical significance to develop a pet food that can not only improve the immunity of pets, but also enhance antioxidant capacity, and at the same time has natural ingredients, is safe and easy to absorb.

[0003] However, there is currently a lack of products that can improve pets' immunity and antioxidant capacity. Summary of the invention

[0004] In view of the problems existing in the prior art, the purpose of the present invention is to provide a pet food with the function of improving immunity and anti-oxidation. By rationally selecting natural active ingredients and combining advanced preparation technology, it aims to effectively enhance the immunity of pets and improve the antioxidant level, thereby delaying aging and improving health.

[0005] At present, medicinal and edible raw materials are gradually introduced into pet food, but due to the complex composition of medicinal and edible extracts, the reasons for their effectiveness in helping to improve immunity and antioxidant levels are unclear, resulting in different results (antagonism, synergy or addition) after compounding different substances or compounding the same substances in different proportions. The inventor screened and obtained a strain of Lactobacillus paracasei ZYhyy-004. Based on the multi-dimensional mechanism of action of postbiotics and its potential advantages in the fields of animal immunity and antioxidant, it is expected to find substances that can be used in combination with Lactobacillus paracasei ZYhyy-004 described in this application to achieve synergistic effects.

[0006] The objective of the present invention is achieved through the following technical solutions: The first aspect of the present invention provides a pet food having immunity-enhancing and antioxidant functions, comprising the following components in percentage by weight: Lactobacillus paracasei postbiotics 0.1%~3%; Eucommia leaf extract 0.5%~2.5% Pumpkin seed superfine powder 0.5%~4%; The Lactobacillus paracasei was named ZYhyy-004, and the strain was deposited in the General Microbiological Center of the China Microbiological Culture Management Committee and was classified as Lacticaseibacillus paracasei , the deposit number is CGMCC No.31554, and the deposit date is August 5, 2024; the Lactobacillus paracasei postbiotics are prepared by inactivating, concentrating and freeze-drying the bacterial liquid of Lactobacillus paracasei ZYhyy-004; The amount of the postbiotics added by Lactobacillus paracasei is equivalent to the amount of the probiotics added before inactivation of 1×10 9 ~1×10 11 CFU / kg pet food.

[0007] Furthermore, the preparation of the Eucommia leaf extract comprises the following steps: Extracting Eucommia ulmoides leaves: mixing the ground Eucommia ulmoides leaf powder with a solvent in proportion and soaking the mixture; placing the mixed solvent in an ultrasonic bath for ultrasonic-assisted extraction, wherein the ultrasonic extraction conditions are set at 40-60°C, the ultrasonic power is 200-400W, and the extraction time is 20-40 minutes; then, filtering the extract to remove solid impurities and obtain a clear extract; Concentrating the extract: using a rotary evaporator to concentrate the extract and remove the solvent to obtain a high-concentration Eucommia leaf extract; Separation and purification of the extract: using a solid phase extraction column to elute and separate the high-concentration Eucommia leaf extract, and collecting the eluate containing the target component; Concentration and drying: the eluate is concentrated, the organic solvent is removed, and finally the eucommia leaf extract is obtained by drying.

[0008] Furthermore, the preparation of the pumpkin seed superfine powder comprises the following steps: The pumpkin seeds are dried and crushed into coarse powder, and then coarsely screened with a 50-mesh screen to remove impurities, and then ultrafinely ground for 20 to 40 minutes to grind them into fine powder with a particle size of 1 to 10 microns, and finally obtain pumpkin seed ultrafine powder.

[0009] Furthermore, the pet food is a pet nutritional paste, which also includes basic raw materials and additives; The basic raw materials are calculated by weight and include: 230-280 parts of purified water, 80-150 parts of maltose, 130-160 parts of chicken meal, 40-60 parts of beef liver powder, 40-60 parts of fish meal, 130-160 parts of fish oil, 20-30 parts of linseed oil, 20-30 parts of coconut oil, 40-60 parts of whole milk powder, 20-30 parts of beet pulp powder, and 20-30 parts of oat flour.

[0010] Furthermore, the additives include: Vitamin A, Vitamin B1, Vitamin B2, Vitamin B6, Vitamin B12, Vitamin D3, Vitamin E, Niacin, Folic Acid, Calcium D-Pantothenate, Potassium Iodide, Zinc Sulfate, Manganese Sulfate, Magnesium Sulfate, Copper Sulfate, Ferrous Lactate, Sodium Alginate.

[0011] The second aspect of the present invention provides a method for preparing the pet food according to the first aspect, comprising the following steps: (1) Heat the oil: Mix fish oil, linseed oil and coconut oil and heat to 45-55°C; (2) Mixing protein and carbohydrate: adding protein source including chicken meal, fish meal, and beef liver powder to the heated oil in step (1), stirring evenly to ensure that the protein is fully dissolved, and obtaining an oil and protein mixture; then, slowly adding carbohydrate powder including purified water, sodium alginate, maltose, whole milk powder, beet pulp powder, and oatmeal powder to the oil and protein mixture, and continuing to stir until a viscous and uniform mixture is formed; (3) Adding vitamins and minerals: Add the remaining additives to the mixture obtained in step (2), homogenize and emulsify at 40-50° C. for 55-65 minutes to ensure complete dissolution and uniform distribution, and obtain the first emulsified material; (4) Adding functional ingredients: Add Lactobacillus paracasei postbiotics, Eucommia ulmoides leaf extract and pumpkin seed ultrafine powder to the first emulsified material, and homogenize and emulsify at 40-50° C. for 25-35 minutes to obtain a pet nutritional paste product with immunity-enhancing and antioxidant functions.

[0012] The third aspect of the present invention provides the use of the pet food described in the first aspect, or the pet nutritional paste product prepared by the method described in the second aspect, in the preparation of products for improving pet immunity and anti-oxidation function.

[0013] Furthermore, the pet includes a cat, a dog, a rabbit, a piglet, a mouse, a mink, a golden bear, a chinchilla, a guinea pig or a meerkat.

[0014] Furthermore, the method for improving the immunity and antioxidant function of pets includes: (1) Simultaneously increase the levels of immune-related indicators IgG, IgM and IgA, and anti-inflammatory indicator IL-10; (2) At the same time, it reduces the levels of inflammatory factor-related indicators IL-6, TNF-α and IL-1β; (3) At the same time, it increases the levels of antioxidant-related indicators such as SOD, T-AOC, GSH-Px and CAT. Strain deposit information:

[0015] Lactobacillus paracasei was named ZYhyy-004, and the strain was deposited in the General Microbiology Center of China Microbiological Culture Management Committee (CGMCC), address: No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing; Lacticaseibacillus paracasei The deposit number is CGMCC No. 31554, and the deposit date is August 5, 2024. Compared with the prior art, the present invention has the following beneficial effects:

[0016] 1. This application compounds Lactobacillus paracasei ZYhyy-004 postbiotics, Eucommia leaf extract and pumpkin seed superfine powder for the preparation of pet food, which can play a synergistic role, effectively enhance the immunity of pets, improve the antioxidant level, thereby delaying aging and enhancing health; 2. The preparation process of the pet food with immunity-enhancing and antioxidant functions described in the present application is simple, cost-effective, and easy to use; 3. In the pet food of the present invention, the Lactobacillus paracasei ZYhyy-004 postbiotics are prepared by inactivating, concentrating and freeze-drying the bacterial liquid of Lactobacillus paracasei ZYhyy-004. The probiotics are inactive and long-term use will not affect the intestinal microecological balance of the pet, nor will it cause immune imbalance or allergic reactions, thereby avoiding the unstable factors caused by the storage and colonization of live bacteria. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] The present invention will be further described below in conjunction with the accompanying drawings and embodiments: Figure 1 This is a comparison chart of the weight gain results of pet cats in different groups; among the groups, different lowercase letters in each group indicate statistically significant differences ( P < 0.05), the same below; Figure 2 A comparison chart of feces scores for different groups of pet cats; Figure 3 The figure is the measurement result of serum immune indexes of pet cats in different groups; Figure 4 This is a graph showing the results of measuring serum inflammatory indicators in different groups of pet cats; Figure 5 This is a graph showing the measurement results of serum antioxidant indicators in different groups of pet cats. DETAILED DESCRIPTION

[0018] The examples are provided to better illustrate the present invention, but the present invention is not limited to the examples. Therefore, those skilled in the art may make non-essential improvements and adjustments to the implementation scheme according to the above invention content, which still fall within the protection scope of the present invention.

[0019] The endpoints and any values ​​of the ranges disclosed in this article are not limited to the precise ranges or values, and these ranges or values ​​should be understood to include values ​​close to these ranges or values. For numerical ranges, the endpoint values ​​of each range, the endpoint values ​​of each range and the individual point values, and the individual point values ​​can be combined with each other to obtain one or more new numerical ranges, which should be considered as specifically disclosed in this article.

[0020] The present invention will be described in detail below by way of examples. It should be understood that the following examples are only used to further explain and illustrate the content of the present invention in detail, and are not used to limit the present invention. Example 1

[0021] This embodiment provides a pet nutritional cream with immunity-enhancing and anti-oxidation functions, the formula of which is: (1) Basic ingredients: 250 parts of purified water, 100 parts of maltose, 150 parts of chicken meal, 50 parts of beef jerky, 50 parts of fish meal, 150 parts of fish oil, 25 parts of linseed oil, 25 parts of coconut oil, 50 parts of whole milk powder, 25 parts of beet pulp powder, and 25 parts of oatmeal powder.

[0022] (2) Additive ingredients: Vitamin A 20000 IU / kg, Vitamin B1 320 mg / kg, Vitamin B2 30 mg / kg, Vitamin B6 120 mg / kg, Vitamin B12 25 mg / kg, Vitamin D3 4000 IU / kg, Vitamin E 300 IU / kg, Niacin 50 mg / kg, Folic acid 20.6 mg / kg, D-calcium pantothenate 50 mg / kg, Potassium iodide 26 mg / kg, Zinc sulfate 150 mg / kg, Manganese sulfate 21 mg / kg, Magnesium sulfate 0.01%, Copper sulfate 36 mg / kg, Ferrous lactate 50 mg / kg, Sodium alginate 1%.

[0023] (3) Functional ingredients: 2% postbiotics from Lactobacillus paracasei ZYhyy-004, 0.5% Eucommia ulmoides leaf extract, and 2% ultrafine pumpkin seed powder.

[0024] The Lactobacillus paracasei ZYhyy-004 postbiotics are prepared by inactivating, concentrating and freeze-drying the Lactobacillus paracasei ZYhyy-004 bacterial solution; the amount of the Lactobacillus paracasei postbiotics added is equivalent to the amount of the probiotics added before inactivation of 1×10 10 CFU / kg pet nutritional paste.

[0025] The preparation of the Eucommia ulmoides leaf extract comprises the following steps: (1) Raw material preparation: Wash the Eucommia leaves, remove impurities, and then dry them. Take a certain amount of Eucommia leaves (usually 50-100 g) and grind them into coarse powder using a mortar and pestle.

[0026] (2) Extraction of Eucommia ulmoides leaves: Mix the ground Eucommia ulmoides leaf powder with a solvent (methanol / water 70:30) in a ratio of 1:10 and soak; place the mixed solvent in an ultrasonic bath for ultrasonic-assisted extraction; the ultrasonic extraction conditions are set at 50°C, the ultrasonic power is 300 W, and the extraction time is 30 minutes; then, filter the extract and remove solid impurities to obtain a clear extract; this extraction technology can effectively shorten the extraction time, effectively improve the extraction rate, reduce the impurity content, and improve the extraction rate and the retention rate of active ingredients.

[0027] (3) Concentrating the extract: Use a rotary evaporator to concentrate the extract and remove the solvent to obtain a high-concentration Eucommia leaf extract.

[0028] (4) Separation and purification of the extract: Use a solid phase extraction (SPE) column to elute and separate the high-concentration Eucommia leaf extract, and collect the eluate containing the target component. Specifically: Activation of solid phase extraction (SPE) column: First, use an appropriate amount of methanol to elute the SPE column to activate the column. Then, use deionized water to fully elute to ensure that the column is completely wet.

[0029] Sample loading: The concentrated Eucommia ulmoides leaf extract was loaded through an SPE column (C18 column, Waters Corporation). In order to improve the extraction efficiency, the adsorption process of the sample can be accelerated by a decompression device to ensure that all target components can be effectively adsorbed onto the solid phase.

[0030] Elution step: Use appropriate solvents to remove possible impurities and non-target components. Use a mixed solvent of water / methanol (80:20) for preliminary elution. Continue to use a stronger organic solvent (100% methanol) for final elution to obtain an eluent containing the target components.

[0031] (5) Concentration and drying: The eluate is concentrated, the organic solvent is removed, and finally the Eucommia ulmoides leaf extract is obtained by drying.

[0032] The preparation of the pumpkin seed superfine powder comprises the following steps: The pumpkin seeds are dried and crushed into coarse powder, and then coarsely screened with a 50-mesh screen to remove impurities, and then ultrafinely ground for 30 minutes to grind them into fine powder with a particle size of 1 to 10 microns, finally obtaining pumpkin seed ultrafine powder.

[0033] The preparation method of the pet nutritional paste is: (1) Heating oils: Mix fish oil, linseed oil, and coconut oil and heat to 50°C; at this point the oils begin to become easier to mix and help dissolve certain fat-soluble components.

[0034] (2) Mixing protein and carbohydrates: Add protein sources including chicken meal, fish meal, and beef liver powder to the heated oil and stir evenly to ensure that the protein is fully dissolved to obtain an oil and protein mixture; then, slowly add carbohydrate powder including purified water, sodium alginate, maltose, whole milk powder, beet pulp powder, and oatmeal powder to the oil and protein mixture and continue stirring until a viscous and uniform mixture is formed.

[0035] (3) Adding vitamins and minerals: Add the remaining additives to the mixture and homogenize and emulsify at 45°C (to ensure that heat-sensitive ingredients are not destroyed) for 60 minutes to ensure complete dissolution and even distribution to obtain the first emulsified material.

[0036] (4) Adding functional ingredients: Add Lactobacillus paracasei ZYhyy-004 postbiotics, Eucommia ulmoides leaf extract and pumpkin seed ultrafine powder to the first emulsified material, and homogenize and emulsify at 40-50° C. for 25-35 minutes to obtain a pet nutritional paste product with immunity-enhancing and antioxidant functions. Example 2

[0037] This embodiment provides another pet nutritional paste product, which is different from Example 1 in that the Lactobacillus paracasei ZYhyy-004 postbiotic is not added therein. Example 3

[0038] This embodiment provides another pet nutritional cream product, which is different from the embodiment 1 in that the added amount of eucommia leaf extract is 1%, and the added amount of pumpkin seed superfine powder is 4%. Comparative Example 1

[0039] This comparative example provides another pet nutritional cream product, which is different from Example 1 in that the added amount of Eucommia ulmoides leaf extract is 2.5%, and no pumpkin seed superfine powder is added. Comparative Example 2

[0040] This comparative example provides another pet nutritional cream product, which is different from Example 1 in that the added amount of pumpkin seed superfine powder is 2.5%, and no eucommia leaf extract is added. Comparative Example 3

[0041] This comparative example provides another pet nutritional cream product, which is different from Example 1 in that the added amount of Eucommia leaf extract is 0.25%, and the added amount of pumpkin seed superfine powder is 1%. Comparative Example 4

[0042] This comparative example provides another pet nutritional cream product, which is different from Example 1 in that the eucommia leaf extract is replaced with a commercially available eucommia leaf extract.

[0043] The commercially available Eucommia ulmoides leaf extract was purchased from Shaanxi Haochen Biotechnology Co., Ltd. Test example: The effects of different pet nutritional paste products on the immune ability of pet cats

[0044] In order to explore the effects of pet nutritional paste products with different compound systems on the immune ability of pets, this test case takes pet cats as an example and conducts the following animal experiments.

[0045] The experiment selected 48 cats and randomly divided them into eight groups, each with 6 cats, namely blank control group, embodiment 1, embodiment 2, embodiment 3, comparative example 1, comparative example 2, comparative example 3, comparative example 4, and ensured that there were no significant differences in the cats in each group. Among them, the blank control group was fed with a blank nutritional paste without adding Lactobacillus paracasei ZYhyy-004 postbiotics, Eucommia leaf extract and pumpkin seed superfine powder every day after surgery; the other groups were fed with the pet nutritional paste products described in the corresponding embodiments or comparative examples. The pre-feeding period was 7 d, and the formal test period was 28 d. During the test, it was ensured that each group of cats was in the same growth environment, that factors such as temperature and humidity were consistent, and that their drinking water sources were sufficient and clean. During the test period, the mental state and feeding status of all pet cats were observed to observe whether there were adverse reactions such as diarrhea and vomiting. 1. Determination of cat weight gain

[0046] Determination method: On the 0th and 28th days of the test, the pet cats of the control group, Examples 1-3 and Comparative Examples 1-4 were weighed, and the weight gain on the 28th day of the test was calculated compared with that on the 0th day of the test.

[0047] according to Figure 1 The results show that compared with the other groups, the control group 1 with only 2.5% of eucommia leaf extract and Lactobacillus paracasei added and the control group 2 with only 2.5% of pumpkin seed superfine powder and Lactobacillus paracasei ZYhyy-004 postbiotic added showed lower weight gain on the 28th day of the experiment, which may be poor for promoting the growth of pet cats in the short term, indicating that the groups with only eucommia leaf extract or pumpkin seed superfine powder added were not as good as the groups with both components added in promoting pet weight gain.

[0048] Overall, the solution of adding only eucommia leaf extract or pumpkin seed superfine powder alone (Comparative Examples 1 and 2) did not show a significant advantage in weight gain, while Example 1, which added 0.5% eucommia leaf extract, 2% pumpkin seed superfine powder and 2% Lactobacillus paracasei ZYhyy-004 postbiotics at the same time, and Example 3, which added 1% eucommia leaf extract, 4% pumpkin seed superfine powder and 2% Lactobacillus paracasei ZYhyy-004 postbiotics at the same time, showed higher weight gain on the 28th day of the experiment; at the same time, Example 2, which did not add Lactobacillus paracasei ZYhyy-004 postbiotics but only added 0.5% eucommia leaf extract and 2% pumpkin seed superfine powder, showed lower weight gain than Example 1 and Example 3. The results showed that the simultaneous addition of 0.5% eucommia leaf extract, 2% pumpkin seed superfine powder and Lactobacillus paracasei ZYhyy-004 postbiotics, or 1% eucommia leaf extract, 4% pumpkin seed superfine powder and Lactobacillus paracasei ZYhyy-004 postbiotics was more conducive to weight gain and achieved a significant synergistic effect on the growth performance of pet cats. 2. Determination of cat fecal score

[0049] Evaluation method: On the 28th day of the test, the quality of cat feces was scored by the same professional, and a comprehensive score was given to cat feces based on a 1-5 point scale and the scoring criteria in Table 1.

[0050]

[0051] In the daily feeding process, pet cats are easily affected by the external environment, which leads to the invasion of pathogens and disorder of intestinal flora, thus causing diarrhea and weight loss. Therefore, effective prevention and control of diarrhea is crucial to the health of pet cats. Fecal score is an important indicator for evaluating the health status of pet cats. A high fecal score will cause the pet cat to have diarrhea symptoms. Figure 2 It can be seen that there is still no significant difference between the comparative example 3 with the addition of 0.25% eucommia leaf extract, 1% pumpkin seed superfine powder and Lactobacillus paracasei ZYhyy-004 postbiotics and the blank control group, indicating that the comparative example 3 is less effective in improving the fecal score. This may be because the amount of effective ingredients added is too small to achieve the effect of improving the fecal score of pets.

[0052] Compared with the blank control group, the fecal score of pet cats significantly decreased in Example 1, which added 0.5% eucommia leaf extract, 2% pumpkin seed superfine powder and Lactobacillus paracasei ZYhyy-004 postbiotics, and Example 3, which added 1% eucommia leaf extract, 4% pumpkin seed superfine powder and Lactobacillus paracasei ZYhyy-004 postbiotics at the same time, indicating that Example 1 and Example 3 can significantly reduce the fecal score, improve the symptoms of diarrhea and soft stool in pet cats, and the ratio of 0.5% eucommia leaf extract, 2% pumpkin seed superfine powder and 2% Lactobacillus paracasei ZYhyy-004 postbiotics or 1% eucommia leaf extract, 4% pumpkin seed superfine powder and 2% Lactobacillus paracasei ZYhyy-004 postbiotics has achieved significant synergistic effects. Analysis and judgment may be due to the fact that after the effective ingredients exceed the optimal dose, they interfere with the absorption of other nutrients, leading to nutritional imbalance. The effect of the formula effective ingredients is no longer enhanced, and may even have a counter-effect. The addition of effective ingredients should be based on appropriate amount and science, rather than pursuing high content. Excessive addition may bring health risks or even be counterproductive. When choosing food for pets, you should focus on comprehensive and balanced nutrition and adjust it according to the individual needs of the pet. 3. Determination of serum immune indicators of pet cats

[0053] Evaluation method: The specific determination method of immunoglobulin A (IgA), immunoglobulin G (IgG) and immunoglobulin M (IgM) in pet cat serum shall be tested according to the instructions of the kit (Nanjing Jiancheng Bioengineering Institute).

[0054] The immune system is an important defense mechanism of the animal body. It can resist foreign pathogens and abnormal cells, protect the body from infection and disease, and has the functions of identifying and initiating immune responses, activating immune cells, and regulating inflammatory responses. Immunoglobulins are very important proteins in the immune system, which have the function of identifying and neutralizing pathogens (such as bacteria, viruses, etc.). IgA, IgG and IgM are the three main types of immunoglobulins, which play different roles in the immune defense of pets. IgA mainly acts on the mucosal surface to protect immune barrier sites such as the respiratory tract and digestive tract; IgG mainly acts in the blood and is the most common immunoglobulin in the body. It provides long-term immune protection and can be passed to newborn pets through breast milk; IgM is an important member of the rapid immune response to the initial infection and can neutralize pathogens in the early stage of infection. These three immunoglobulins work together to help pets effectively resist the invasion of pathogens at different stages and sites.

[0055] like Figure 3It can be seen that compared with Comparative Example 4, the contents of immune-related indicators IgG, IgM and IgA in the serum of pet cats in Example 1 group were significantly increased by 41.56%, 14.57% and 36.29%, respectively. The above results further show that compared with the commercially available Eucommia leaf extract, the Eucommia leaf extract in Example 1 of this product has a significant promoting effect in enhancing the immune function of pet cats. 4. Determination of Serum Inflammatory Indexes in Pet Cats

[0056] Evaluation method: The specific determination method of serum interleukin-6 (IL-6), interleukin-10 (IL-10), interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) indicators was tested according to the instructions of ELISA kit (Shanghai ELISA Biotechnology Co., Ltd.).

[0057] L-6, TNF-α, IL-1β and IL-10 are four important cytokines that play a key role in the pet's immune system. Among them, IL-6, TNF-α and IL-1β are mainly pro-inflammatory cytokines, which promote the production of inflammation in the immune response and help eliminate pathogens, but when overactive, they may cause chronic inflammation or tissue damage. IL-10 is an anti-inflammatory cytokine that can inhibit excessive immune responses, help maintain the balance of the immune system, and prevent the occurrence of autoimmune diseases. These cytokines interact with each other in the pet's immune system to maintain the appropriate intensity and duration of the immune response, ensuring that the pet can effectively respond to infections, injuries, and other immune challenges.

[0058] Depend on Figure 4 It can be seen that compared with Comparative Example 4, the levels of inflammatory factor-related indicators IL-6, TNF-α and IL-1β in the serum of the pet cats in Example 1 group were significantly reduced by 10.91%, 15.41% and 14.18%, respectively, and the anti-inflammatory indicator IL-10 in the serum increased significantly by 14.24%, indicating that the inflammation in the pets was significantly alleviated. The above results further show that compared with the commercially available eucommia leaf extract, the eucommia leaf extract in Example 1 of this product is more effective in relieving inflammation in pets and improving the physical health of pets. 5. Determination of serum antioxidant indexes in pet cats

[0059] Evaluation method: The specific determination method of superoxide dismutase (SOD), total antioxidant capacity (T-AOC), glutathione peroxidase (GSH-Px) and catalase (CAT) in serum was tested according to the instructions of the kit (Nanjing Jiancheng Bioengineering Institute).

[0060] The antioxidant system is one of the body's defense mechanisms. Immune cells release oxidants when clearing pathogens. Oxidative factors in the body reduce the effects of oxidative stress on the immune system by clearing free radicals and oxidants, thereby enhancing immune function. SOD converts superoxide radicals into hydrogen peroxide and oxygen, GSH-Px can specifically catalyze the reduction reaction of reduced glutathione to hydrogen peroxide, and T-AOC reflects the total level of antioxidants in the body and the overall antioxidant status, which together resist oxidative stress and protect the body from oxidative damage. CAT decomposes hydrogen peroxide to exert antioxidant protection, reduce oxidative damage, and plays a key role in maintaining intracellular redox balance and supporting immune responses. Figure 5 It can be seen that compared with Comparative Example 4, the contents of antioxidant-related indicators SOD, T-AOC, GSH-Px and CAT in the serum of pet cats in Example 1 group were significantly increased by 16.20%, 19.95%, 11.59% and 16.54%, respectively, indicating that compared with the commercially available Eucommia leaf extract, the Eucommia leaf extract in Example 1 of this product has a significant promoting effect in enhancing the antioxidant capacity of pet cats.

[0061] Finally, it should be noted that the above is only used to illustrate the technical solution of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the preferred arrangement scheme, a person skilled in the art should understand that the technical solution of the present invention may be modified or replaced by equivalents without departing from the spirit and scope of the technical solution of the present invention.

Claims

1. A pet food with immunity-enhancing and antioxidant functions, characterized in that: The pet food comprises the following components in weight percentage: Lactobacillus paracasei postbiotics 0.1%~3%; Eucommia leaf extract 0.5%~2.5% Pumpkin seed superfine powder 0.5%~4%; The Lactobacillus paracasei was named ZYhyy-004, and the strain was deposited in the General Microbiological Center of the China Microbiological Culture Management Committee and was classified as Lacticaseibacillus paracasei , the deposit number is CGMCC No.31554, and the deposit date is August 5, 2024; The Lactobacillus paracasei postbiotics are prepared by inactivating, concentrating and freeze-drying the bacterial liquid of Lactobacillus paracasei ZYhyy-004; The amount of the postbiotics of Lactobacillus paracasei added is equivalent to the amount of the probiotics added before inactivation of 1×10 9 ~1×10 11 CFU / kg pet food.

2. The pet food according to claim 1, characterized in that The preparation of the Eucommia ulmoides leaf extract comprises the following steps: (1) Raw material preparation: Wash the Eucommia leaves, remove impurities, dry them, and grind them into coarse powder using a mortar and pestle; (2) Extraction of Eucommia ulmoides leaves: Mix the ground Eucommia ulmoides leaf powder with a solvent in a certain proportion and soak them; put the mixed solvent into an ultrasonic bath for ultrasonic-assisted extraction; the ultrasonic extraction conditions are set at 40-60°C, the ultrasonic power is 200-400W, and the extraction time is 20-40 minutes; then, filter the extract to remove solid impurities and obtain a clear extract; (3) Concentrating the extract: using a rotary evaporator to concentrate the extract and remove the solvent to obtain a high-concentration Eucommia leaf extract; (4) separation and purification of the extract: using a solid phase extraction column to elute and separate the high-concentration Eucommia leaf extract, and collecting the eluate containing the target component; (5) Concentration and drying: The eluate is concentrated, the organic solvent is removed, and finally the Eucommia ulmoides leaf extract is obtained by drying.

3. The pet food according to claim 1, characterized in that The preparation of the pumpkin seed superfine powder comprises the following steps: The pumpkin seeds are dried and crushed into coarse powder, and then coarsely screened with a 50-mesh screen to remove impurities, and then ultrafinely ground for 20 to 40 minutes to grind them into fine powder with a particle size of 1 to 10 microns, and finally obtain pumpkin seed ultrafine powder.

4. The pet food according to claim 1, characterized in that The pet food is a pet nutritional paste, which also includes basic raw materials and additives; The basic raw materials are calculated by weight and include: 230-280 parts of purified water, 80-150 parts of maltose, 130-160 parts of chicken meal, 40-60 parts of beef liver powder, 40-60 parts of fish meal, 130-160 parts of fish oil, 20-30 parts of linseed oil, 20-30 parts of coconut oil, 40-60 parts of whole milk powder, 20-30 parts of beet pulp powder, and 20-30 parts of oat flour.

5. The pet food according to claim 4, characterized in that The additives include: Vitamin A, Vitamin B1, Vitamin B2, Vitamin B6, Vitamin B12, Vitamin D3, Vitamin E, Niacin, Folic Acid, Calcium D-Pantothenate, Potassium Iodide, Zinc Sulfate, Manganese Sulfate, Magnesium Sulfate, Copper Sulfate, Ferrous Lactate, Sodium Alginate.

6. A method for preparing pet food as claimed in claim 5, characterized in that: The preparation method comprises the following steps: (1) Heat the oil: Mix fish oil, linseed oil and coconut oil and heat to 45-55°C; (2) Mixing protein and carbohydrate: adding protein source including chicken meal, fish meal, and beef liver powder to the heated oil in step (1), stirring evenly to ensure that the protein is fully dissolved, and obtaining an oil and protein mixture; then, slowly adding carbohydrate powder including purified water, sodium alginate, maltose, whole milk powder, beet pulp powder, and oatmeal powder to the oil and protein mixture, and continuing to stir until a viscous and uniform mixture is formed; (3) Adding vitamins and minerals: Add the remaining additives to the mixture obtained in step (2), homogenize and emulsify at 40-50° C. for 55-65 minutes to ensure complete dissolution and uniform distribution, and obtain the first emulsified material; (4) Adding functional ingredients: Add Lactobacillus paracasei postbiotics, Eucommia ulmoides leaf extract and pumpkin seed ultrafine powder to the first emulsified material, and homogenize and emulsify at 40-50° C. for 25-35 minutes to obtain a pet nutritional paste product with immunity-enhancing and antioxidant functions.

7. Use of the pet food according to any one of claims 1 to 5, or the pet nutritional paste product prepared by the method according to claim 6 in the preparation of products for improving pet immunity and antioxidant function.

8. The use according to claim 7, characterized in that: The pets include cats, dogs, rabbits, piglets, mice, minks, chinchillas, rats, guinea pigs or meerkats.

9. The use according to claim 7, characterized in that: The pet immunity and antioxidant function mentioned above include: (1) Simultaneously increase the levels of immune-related indicators IgG, IgM and IgA, and anti-inflammatory indicator IL-10; (2) At the same time, it reduces the levels of inflammatory factor-related indicators IL-6, TNF-α and IL-1β; (3) At the same time, it increases the levels of antioxidant-related indicators such as SOD, T-AOC, GSH-Px and CAT.

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