Low-protein feed for broiler chickens and application of low-protein feed

By adjusting the crude protein, calcium, phosphorus and electrolyte content in broiler low-protein feed, the problem of changes in the demand for these elements of broiler under low-protein feed conditions was solved, and the effect of improving broiler growth performance and bone development was achieved.

CN119817715APending Publication Date: 2025-04-15INSTITUTE OF ANIMAL SCIENCES OF CHINESE ACADEMY OF AGRICULTURAL SCIENCES
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Patent Information

Application Number
CN202411912356.2
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-12-24
Publication Date
2025-04-15

AI Technical Summary

Technical Problem

Under low-protein feed conditions, the demand for calcium, phosphorus, electrolytes and trace elements of broilers has changed. The existing technology is difficult to meet the balanced demand of calcium, phosphorus, electrolytes and trace elements, affecting the growth performance and bone development of broilers.

Method used

By adjusting the crude protein content, calcium and non-phytate phosphorus content in broiler low-protein feed, as well as electrolyte equilibrium value, restricted amino acids are used to ensure the balance of calcium, phosphorus and electrolytes and improve the utilization rate of feed.

Benefits of technology

It effectively improves the growth performance, bone development, nitrogen deposition and metabolic utilization and emission of trace mineral elements of broilers, improves the efficiency of feed utilization, and improves the production efficiency of broilers.

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Abstract

The invention provides a broiler low-protein feed and application thereof, the broiler low-protein feed comprises restrictive amino acid, the content of crude protein is 18-20 wt%, the content of calcium is 0.7-1.0 wt%, the content of non-phytic acid phosphorus is 0.3-0.6 wt%, and the electrolyte equilibrium value is 200-300 mmol / kg. The invention also provides a broiler low-protein feed, which is prepared from the following components in parts by weight: 55 to 65 parts of corn, 30 to 35 parts of soybean meal, 2 to 4 parts of soybean oil, 0.2 to 0.4 part of table salt, 0.15 to 0.25 part of methionine, 0.1 to 0.15 part of lysine, 0.02 to 0.05 part of threonine, 0.01 to 0.03 part of tryptophan, 2 to 4 parts of calcium and phosphorus components, 1 to 1.5 parts of electrolyte components and / or 0.2 to 0.4 part of trace mineral element components and 0.20 to 0.35 part of trace components. The broiler low-protein feed provided by the invention can meet the requirements of calcium phosphorus, electrolyte and trace mineral elements in the production process of yellow-feathered broilers, and can effectively improve the growth performance, skeletal development, nitrogen deposition, metabolic utilization and emission of macromineral elements and deposition of trace mineral elements of the yellow-feathered broilers.
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Description

Technical Field

[0001] The invention relates to the technical field of chicken feed, and in particular to a low-protein feed for broiler chickens and application thereof. Background Art

[0002] Since excessive intake of protein feed will increase production costs and the emission of nitrogen-containing pollutants, my country is currently vigorously promoting low-protein feed technology. Studies have shown that reducing the crude protein level in the feed will affect the metabolism and utilization of mineral elements (such as macro-mineral elements), but the demand for mineral elements and electrolytes in broiler and other livestock and poultry feed under low-protein feed conditions is still unclear.

[0003] Mineral elements have important biological functions and play an important role in regulating the growth and metabolism of animals; electrolytes play an important role in maintaining osmotic pressure, regulating acid-base balance, regulating water metabolism and other physiological processes. Moreover, the metabolic regulation between different mineral elements is closely related, especially the demand for macro-mineral elements Ca, P and electrolytes in broilers affects each other, and there is a complex interaction between trace mineral elements Cu, Fe, Mn and Zn. Therefore, it is urgent to further study the calcium-phosphorus and electrolyte balance mode and the trace element balance mode of low-protein diets for broilers. By regulating multiple mineral elements (Ca, P, Na, K, Cl, Cu, Fe, Mn, Zn), the production efficiency of broiler feeding under low-protein diets can be further improved, providing a theoretical basis for the promotion and application of low-protein diets in broiler production. Summary of the invention

[0004] Studies have shown that a reduction in crude protein levels in broiler diets will affect the metabolism and utilization of mineral elements (such as macro-mineral elements). However, the balance pattern of multiple mineral elements (especially trace mineral elements), calcium-phosphorus requirements, and electrolyte balance patterns in low-protein broiler diets have not been reported. The absorption of dietary Ca, P, Na, K, Cl, Cu, Fe, Mn, and Zn by broilers is closely related to protein. On the one hand, amino acids and small peptides produced by dietary protein digestion can promote the absorption of mineral elements; on the other hand, reducing the amount of soybean meal in the diet can reduce the content of anti-nutritional factors in the diet that affect the absorption of mineral elements, such as soybean lectin, phytic acid, and fiber.

[0005] In summary, under low-protein diet conditions, the requirements of yellow-feathered broilers for Ca, non-phytate phosphorus (NPP), electrolytes and trace elements may change, and it is no longer appropriate to set the dietary calcium, phosphorus, electrolyte and trace element levels according to the Chinese standard for nutrient requirements of yellow-feathered broilers (NY / T 3645-2020). Therefore, it is urgent to develop the mineral element and electrolyte preparation technology for low-protein feed for yellow-feathered broilers, so as to provide a basis for the preparation and application of mineral elements and electrolytes in low-protein diets in broiler production.

[0006] The present invention aims to solve at least one of the above technical problems to a certain extent. To this end, one object of the present invention is to provide a low-protein feed for broilers, comprising limiting amino acids, wherein the crude protein content is 18-20wt%, the calcium and non-phytate phosphorus contents are 0.7-1.0wt% and 0.3-0.6wt%, respectively, and the electrolyte balance value is 200-300 mmol / kg.

[0007] In the low-protein feed for broilers provided by the present invention, the crude protein content in the feed is reduced, and restrictive amino acids are used to effectively improve the feed utilization rate, thereby improving the production performance of the animals; the calcium and non-phytate phosphorus content and the electrolyte balance value are within the above range, which is beneficial to meet the requirements of low-protein broilers (such as yellow-feathered broilers) for the levels of calcium, phosphorus, electrolytes and mineral elements in the feed during the production process, improve the feed utilization rate, and further improve production performance, such as growth performance (i.e. weight gain), bone development, nitrogen deposition, and the metabolic utilization and excretion of mineral elements (such as major mineral elements). ‌

[0008] In some embodiments, the crude protein content in the broiler low-protein feed is 18.5-19.5wt%, the calcium and non-phytate phosphorus contents are 0.8-0.9wt% and 0.4-0.5wt%, respectively, and the electrolyte balance value is 250-300 mmol / kg. Preferably, when the crude protein content, calcium, phosphorus and electrolyte balance values ​​in the broiler low-protein feed are within this range, better results can be achieved in terms of growth performance (i.e., weight gain), bone development, nitrogen deposition, metabolic utilization and discharge of mineral elements (such as macro-mineral elements), etc.

[0009] In some embodiments, the broiler low-protein feed also includes Cu, Fe, Mn, and Zn in contents of 8-13, 95-120, 83-120, and 61-90 mg / kg, respectively, which can significantly improve the growth performance, antioxidant properties, trace mineral element deposition, and feed utilization of broilers (such as yellow-feathered broilers), so as to take into account both growth performance and ecological effects.

[0010] In some embodiments, the limiting amino acid is selected from at least one of methionine, lysine, tryptophan, and threonine.

[0011] Based on the above-mentioned inventive concept, the second object of the present invention is to provide a specific low-protein feed for broilers, which comprises, by weight: 55-65 parts of corn, 30-35 parts of soybean meal, 2-4 parts of soybean oil, 0.2-0.4 parts of salt, 0.15-0.25 parts of methionine, 0.1-0.15 parts of lysine, 0.02-0.05 parts of threonine, 0.01-0.03 parts of tryptophan, 2-4 parts of calcium and phosphorus components, 1-1.5 parts of electrolyte components and / or 0.2-0.4 parts of trace mineral elements components, 0.20-0.35 parts of trace components; the preferred total amount is 100 parts. This formula of low-protein feed uses limiting amino acids such as methionine, lysine, threonine, and tryptophan to reduce the crude protein content in the feed, while effectively improving feed utilization, thereby improving the production performance of animals; at the same time, the calcium-phosphorus content and electrolyte balance value also meet the needs of low-protein broiler chickens (such as yellow-feathered broilers) for calcium, phosphorus, and electrolyte levels in the feed during the production process, thereby improving feed utilization and further improving production performance, such as growth performance (i.e. weight gain), bone development, nitrogen deposition, and metabolic utilization and excretion of major mineral elements.

[0012] In some embodiments, the broiler low-protein feed includes, by weight: 55-60 parts of corn, 30-35 parts of soybean meal, 3-4 parts of soybean oil, 0.2-0.4 parts of salt, 0.15-0.25 parts of methionine, 0.1-0.15 parts of lysine, 0.03-0.05 parts of threonine, 0.01-0.02 parts of tryptophan, 3-4 parts of calcium and phosphorus components, 1-1.5 parts of electrolyte components, and 0.25-0.35 parts of trace ingredients; preferably, the total amount is 100 parts.

[0013] In some embodiments, the electrolyte component includes 1-1.2 parts of sodium bicarbonate and zeolite powder, and based on the weight of the feed, the feed includes 0.4-10 g / kg of the sodium bicarbonate.

[0014] In some embodiments, the calcium-phosphorus component includes 0.5-2 parts of calcium hydrogen phosphate, 0.5-1.5 parts of stone powder, and 0.8-1.6 parts of gravel.

[0015] Based on the above-mentioned inventive concept, the third object of the present invention is to provide another specific low-protein feed for broilers, which comprises, by weight: 60-65 parts of corn, 30-35 parts of soybean meal, 2-3 parts of soybean oil, 0.25-0.35 parts of salt, 0.15-0.20 parts of methionine, 0.10-0.15 parts of lysine, 0.02-0.04 parts of threonine, 0.02-0.03 parts of tryptophan, 2-3 parts of calcium and phosphorus components, 0.20-0.25 parts of trace ingredients (excluding copper, iron, manganese, and zinc), and 0.2-0.4 parts of trace mineral elements (corn starch and copper, iron, manganese, and zinc); preferably, the total amount is 100 parts.

[0016] In some embodiments, the trace mineral element components include copper sulfate, ferrous sulfate, manganese sulfate, zinc sulfate and corn starch. Based on the weight of the feed, the feed includes 0.01-0.06 g / kg of copper sulfate, 0.05-0.4 g / kg of ferrous sulfate, 0.05-0.4 g / kg of manganese sulfate and 0.05-0.4 g / kg of zinc sulfate and 0.20-0.26 parts of corn starch.

[0017] This formula of low-protein broiler feed can meet the calcium, phosphorus and electrolyte level requirements of broilers (such as yellow-feathered broilers) during the production process and improve their growth performance. At the same time, it can also improve the antioxidant properties and trace mineral element deposition of broiler internal organs (such as heart and liver), thereby improving feed utilization efficiency.

[0018] The fourth object of the present invention is to provide the use of the aforementioned broiler low-protein feed in yellow-feathered broilers.

[0019] In some embodiments, the application includes using the broiler low-protein feed to improve at least one of the growth performance, bone development, nitrogen deposition, metabolic utilization and discharge of major mineral elements, and trace element deposition of yellow-feathered broilers.

[0020] The present invention has at least the following technical effects: by controlling the crude protein content, calcium and phosphorus content and electrolyte balance value in the low-protein feed, and also by controlling the content of multiple trace mineral elements in the feed, a low-protein feed for broilers is provided in combination in multiple aspects, so as to meet the needs of low-protein broilers for calcium, phosphorus, electrolytes and trace mineral elements, improve the growth performance and antioxidant capacity of broilers, improve bone performance, N deposition and deposition and utilization of trace mineral elements, etc.; and further by adjusting and optimizing the components and dosages, a specific formula of broiler low-protein feed is provided, which can be used for feeding yellow-feathered broilers, meet the needs of low-protein yellow-feathered broilers for mineral elements (macro-mineral elements and trace mineral elements) and electrolytes, effectively improve the utilization efficiency of the feed, effectively improve the growth performance and antioxidant capacity of low-protein yellow-feathered broilers, improve their bone performance, nitrogen deposition and deposition of trace mineral elements, and safely and efficiently improve the production benefits of yellow-feathered broilers. DETAILED DESCRIPTION

[0021] Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in the field without making any creative work shall fall within the scope of protection of the present invention.

[0022] Given that the demand for mineral elements and electrolytes in the feed of broilers and other livestock and poultry under the current low-protein diet conditions is still unclear, the levels of mineral elements and electrolytes in the low-protein diet of yellow-feathered broilers are mostly still based on my country's Nutrient Requirements Standards for Yellow-feathered Broilers (NY / T 3645-2020).

[0023] The present invention provides a low-protein feed for broilers, wherein the crude protein content is reduced by 1-3wt% compared with the nutrient requirement standard for medium-speed yellow-feathered broilers (NY / T 3645-2020), such as 1%, 2%, 3%, etc. and the range of their composition; limiting amino acids are used to effectively improve feed utilization, and the contents of calcium and non-phytate phosphorus in the feed are controlled to be 0.7-1.0wt% and 0.3-0.6wt%, respectively, and the electrolyte balance value is 200-300. mmol / kg, specifically, the calcium content can be 0.7wt%, 0.8wt%, 0.85wt%, 0.9wt%, 0.95wt%, 1.0wt% and the like and a content range thereof (such as 0.8-0.9wt%), the non-phytic phosphorus content can be 0.3wt%, 0.35wt%, 0.4wt%, 0.45wt%, 0.5wt%, 0.55wt%, 0.6wt% and the like and a content range thereof (such as 0.4-0.5wt%), and the electrolyte balance value can be 200mmol / kg, 250mmol / kg, 300mmol / kg and the like and a range thereof (such as 250-300mmol / kg).

[0024] Wherein, the limiting amino acid is selected from at least one of methionine, lysine, tryptophan and threonine.

[0025] In some embodiments, the broiler low-protein feed may also include Cu, Fe, Mn, and Zn in contents of 8-13, 95-120, 83-120, and 61-90 mg / kg, respectively, to improve growth performance, antioxidant properties, trace mineral element deposition, etc., to obtain better comprehensive performance.

[0026] Specifically, the embodiments of the present invention provide a broiler low-protein feed, which includes, by weight: 55-65 parts of corn, 30-35 parts of soybean meal, 2-4 parts of soybean oil, 0.2-0.4 parts of salt, 0.15-0.25 parts of methionine, 0.1-0.15 parts of lysine, 0.02-0.05 parts of threonine, 0.01-0.03 parts of tryptophan, 3-4 parts of calcium and phosphorus components, 1-1.2 parts of electrolyte components, and 0.25-0.35 parts of trace ingredients. The preferred total amount is 100 parts, that is, the amounts expressed in the above weight parts can also be directly changed to weight percentages.

[0027] In some embodiments, the broiler low-protein feed includes, by weight: 55-60 parts of corn, 30-35 parts of soybean meal, 3-4 parts of soybean oil, 0.2-0.4 parts of salt, 0.15-0.25 parts of methionine, 0.1-0.15 parts of lysine, 0.03-0.05 parts of threonine, 0.01-0.02 parts of tryptophan, 3-4 parts of calcium and phosphorus components, 1-1.5 parts of electrolyte components, and 0.25-0.35 parts of trace ingredients; preferably, the total amount is 100 parts.

[0028] The electrolyte components include sodium bicarbonate and 1-1.2 parts of zeolite powder; based on the weight of the feed, the feed includes 0.4-10g / kg of sodium bicarbonate; the calcium and phosphorus components include 0.5-2 parts of calcium hydrogen phosphate, 0.5-1.5 parts of stone powder, and 0.8-1.6 parts of gravel.

[0029] In some embodiments, the broiler low-protein feed includes, by weight: 60-65 parts of corn, 30-35 parts of soybean meal, 2-3 parts of soybean oil, 0.25-0.35 parts of salt, 0.15-0.20 parts of methionine, 0.10-0.15 parts of lysine, 0.02-0.04 parts of threonine, 0.02-0.03 parts of tryptophan, 2-3 parts of calcium and phosphorus components, 0.20-0.25 parts of trace ingredients (excluding copper, iron, manganese, and zinc), and 0.2-0.4 parts of trace mineral elements (corn starch and copper, iron, manganese, and zinc); preferably, the total amount is 100 parts.

[0030] Wherein, the trace mineral element components include copper sulfate, ferrous sulfate, manganese sulfate, zinc sulfate and corn starch. Based on the weight of the feed, the feed includes 0.01-0.06 g / kg of copper sulfate, 0.05-0.4 g / kg of ferrous sulfate, 0.05-0.4 g / kg of manganese sulfate, 0.05-0.4 g / kg of zinc sulfate and 0.20-0.26 parts of corn starch.

[0031] In some embodiments, the broiler low-protein feed contains electrolyte components, calcium and phosphorus components, and trace mineral elements.

[0032] The preparation method of the broiler low-protein feed is as follows: weigh the raw materials according to the formula and mix them evenly.

[0033] The above-mentioned broiler low-protein feed can be used to raise yellow-feathered broilers, especially to improve the growth performance, bone development, nitrogen deposition, metabolic utilization and discharge of major mineral elements, and deposition of trace mineral elements of yellow-feathered broilers.

[0034] It has been verified that when the contents of copper, iron, zinc and manganese in the feed are 12.99, 120.00, 120.00 and 60.74 mg / kg respectively, it is most beneficial to improve the growth performance of yellow-feathered broilers aged 1-30 days.

[0035] It has been verified that different addition amounts (mg / kg) of copper, iron, manganese and zinc can optimize the activities of different antioxidant enzymes in different tissues. The specific addition amounts and the corresponding tissue antioxidant enzyme activities are shown in the table below.

[0036] It has been verified that different addition amounts of copper, iron, manganese and zinc (mg / kg) can achieve the optimal deposition of different elements in different tissues, see the table below.

[0037] For the sake of simplicity, in the following specific embodiments, only individual combinations are taken as examples, and their comprehensive performance is evaluated as being superior.

[0038] It is worth noting that, unless otherwise specified, the trace mineral elements mentioned include: copper (Cu), iron (Fe), manganese (Mn), zinc (Zn); trace mineral element components include copper sulfate, ferrous sulfate, manganese sulfate, zinc sulfate and corn starch; macro mineral elements include: calcium (Ca), phosphorus (P), etc.; electrolyte components include sodium (Na), potassium (K), chlorine (Cl); trace components include: selenium (Se), iodine (I), choline, multivitamins, etc.

[0039] The sources of the elements / raw materials involved are shown in the table below, and those not mentioned are commercially available feed-grade raw materials.

[0040] Among them, the chlorine content of choline chloride is 12.7%, and 1.8767g / kg choline chloride is added to the feed, that is, the choline chloride content in the feed is 0.18767%, and the chlorine content added to the feed by choline chloride is 0.18767*12.7%=0.0238.

[0041] The present invention is described below with reference to specific embodiments. It should be noted that these embodiments are merely illustrative and do not limit the present invention in any way.

[0042] The test method of the measured value involved in the embodiment is as follows: sampling is performed on-site when preparing the feed, and the sample is ground through a 200-mesh sieve and placed in a plastic bottle for low-temperature storage. The crude protein content of the feed is determined by the conventional Kjeldahl method (GB / T 6432-2018); the calcium content in the feed is determined by a plasma emission spectrometer; the phytic acid phosphorus content in the feed is determined by the ferric chloride precipitation method; and the P content in the tibial ash is determined by the molybdenum yellow spectrophotometry method according to the national standard GB / T 6437-2002. If not specifically described, those skilled in the art can select according to the conditions, parameters, methods, etc. disclosed in the prior art.

[0043] Example 1 The present embodiment provides a low-protein feed for broilers, and the formula composition is as follows: 43.27kg corn, 24.67kg soybean meal, 2.79kg soybean oil, 0.23kg salt, 0.15kg methionine, 0.11kg lysine, 0.03kg threonine, 0.01kg tryptophan, 2.625kg calcium and phosphorus components, and 0.9kg electrolyte components.

[0044] Among them, the calcium and phosphorus components are composed of 0.7200kg of calcium hydrogen phosphate, 0.7050kg of stone powder and 1.200kg of gravel; the electrolyte component is composed of 34.02g of sodium bicarbonate and 865.98g of zeolite powder.

[0045] The percentage content and nutritional composition of each component in the feed provided in this embodiment are shown in Table 1.

[0046] Table 1 Diet composition and nutrient levels of yellow-feathered broiler chickens aged 1-30 days (wt%)

[0047] In Table 1, trace components involve trace mineral elements (Cu, Fe, Mn, Zn) and Se, I, choline and broiler multivitamins; trace components added per kilogram of feed: vitamin A 11000 IU, vitamin D3 4800 IU, vitamin E 70 IU, vitamin K311 mg, vitamin B1 4.45 mg, vitamin B2 9 mg, vitamin B6 6.25 mg, vitamin B12 0.04 mg, calcium pantothenate 21.75 mg, niacinamide 70 mg, folic acid 2.2 mg, biotin 0.3 mg, choline 700 mg, selenium 0.15 mg, iodine 0.35 mg, copper 7 mg, iron 80 mg, manganese 60 mg, zinc 80 mg; except for crude protein, calcium, phosphorus and non-phytate phosphorus which are measured values, all other nutrients are theoretical values.

[0048] The low-protein feed provided in this embodiment has a calcium content of 0.70%, a non-phytic acid phosphorus content of 0.35%, and an electrolyte balance value DEB of 200.

[0049] According to the content of Na, K, and Cl elements multiplied by the conversion coefficient (common elements Na: 435, K: 256, Cl: 282), the contribution value of each electrolyte to DEB is obtained. The electrolyte balance value of the broiler low-protein feed provided in this embodiment is DEB = 0.12*435+0.88*256-(0.27+0.0238)*282=194.6284, which is rounded to 200.

[0050] Example 2 The present embodiment provides a low-protein feed for broiler chickens, which is basically the same as that of Embodiment 1, except that its formula composition is as follows: 43.27 kg of corn, 24.67 kg of soybean meal, 2.79 kg of soybean oil, 0.23 kg of salt, 0.15 kg of methionine, 0.11 kg of lysine, 0.03 kg of threonine, 0.01 kg of tryptophan, 2.625 kg of calcium and phosphorus components, and 0.9 kg of electrolyte components.

[0051] Among them, the calcium and phosphorus components are composed of 0.7200kg of calcium hydrogen phosphate, 0.7050kg of stone powder and 1.200kg of gravel; the electrolyte component is composed of 350.69g of sodium bicarbonate and 549.31g of zeolite powder.

[0052] In this embodiment, the calcium content is 0.7%, the non-phytate phosphorus content is 0.35%, and the DEB value is 250.

[0053] Example 3 The present embodiment provides a low-protein feed for broiler chickens, which is basically the same as that of Embodiment 1, except that its formula composition is as follows: 43.27 kg of corn, 24.67 kg of soybean meal, 2.79 kg of soybean oil, 0.23 kg of salt, 0.15 kg of methionine, 0.11 kg of lysine, 0.03 kg of threonine, 0.01 kg of tryptophan, 2.625 kg of calcium and phosphorus components, and 0.9 kg of electrolyte components.

[0054] Among them, the calcium and phosphorus components are composed of 0.7200kg of calcium hydrogen phosphate, 0.7050kg of stone powder and 1.200kg of gravel; the electrolyte component is composed of 667.36g of sodium bicarbonate and 232.64g of zeolite powder.

[0055] In this embodiment, the calcium content is 0.7%, the non-phytate phosphorus content is 0.35%, and the DEB value is 300.

[0056] Example 4 The present embodiment provides a low-protein feed for broiler chickens, which is basically the same as that of Embodiment 1, except that its formula composition is as follows: 43.27 kg of corn, 24.67 kg of soybean meal, 2.79 kg of soybean oil, 0.23 kg of salt, 0.15 kg of methionine, 0.11 kg of lysine, 0.03 kg of threonine, 0.01 kg of tryptophan, 2.625 kg of calcium and phosphorus components, and 0.9 kg of electrolyte components.

[0057] Among them, the calcium and phosphorus components are composed of 0.9750kg of calcium hydrogen phosphate, 0.7500kg of stone powder and 0.900kg of gravel; the electrolyte component is composed of 34.02g of sodium bicarbonate and 865.98g of zeolite powder.

[0058] In this embodiment, the calcium content is 0.8%, the non-phytate phosphorus content is 0.4%, and the DEB value is 200.

[0059] Example 5 The present embodiment provides a low-protein feed for broiler chickens, which is basically the same as that of Embodiment 1, except that its formula composition is as follows: 43.27 kg of corn, 24.67 kg of soybean meal, 2.79 kg of soybean oil, 0.23 kg of salt, 0.15 kg of methionine, 0.11 kg of lysine, 0.03 kg of threonine, 0.01 kg of tryptophan, 2.625 kg of calcium and phosphorus components, and 0.9 kg of electrolyte components.

[0060] Among them, the calcium and phosphorus components are composed of 0.9750kg of calcium hydrogen phosphate, 0.7500kg of stone powder and 0.900kg of gravel; the electrolyte component is composed of 350.69g of sodium bicarbonate and 549.31g of zeolite powder.

[0061] In this embodiment, the calcium content is 0.8%, the non-phytate phosphorus content is 0.4%, and the DEB value is 250.

[0062] Example 6 The present embodiment provides a low-protein feed for broiler chickens, which is basically the same as that of Embodiment 1, except that its formula composition is as follows: 43.27 kg of corn, 24.67 kg of soybean meal, 2.79 kg of soybean oil, 0.23 kg of salt, 0.15 kg of methionine, 0.11 kg of lysine, 0.03 kg of threonine, 0.01 kg of tryptophan, 2.625 kg of calcium and phosphorus components, and 0.9 kg of electrolyte components.

[0063] Among them, the calcium and phosphorus components are composed of 0.9750kg of calcium hydrogen phosphate, 0.7500kg of stone powder and 0.900kg of gravel; the electrolyte component is composed of 667.36g of sodium bicarbonate and 232.64g of zeolite powder.

[0064] In this embodiment, the calcium content is 0.8%, the non-phytate phosphorus content is 0.4%, and the DEB value is 300.

[0065] Example 7 The present embodiment provides a low-protein feed for broiler chickens, which is basically the same as that of Embodiment 1, except that its formula composition is as follows: 43.27 kg of corn, 24.67 kg of soybean meal, 2.79 kg of soybean oil, 0.23 kg of salt, 0.15 kg of methionine, 0.11 kg of lysine, 0.03 kg of threonine, 0.01 kg of tryptophan, 2.625 kg of calcium and phosphorus components, and 0.9 kg of electrolyte components.

[0066] Among them, the calcium and phosphorus components are composed of 1.1400kg of calcium hydrogen phosphate, 0.8625kg of stone powder and 0.6225kg of gravel; the electrolyte component is composed of 34.02g of sodium bicarbonate and 865.98g of zeolite powder.

[0067] In this embodiment, the calcium content is 0.9%, the non-phytate phosphorus content is 0.45%, and the DEB value is 200.

[0068] Example 8 The present embodiment provides a low-protein feed for broiler chickens, which is basically the same as that of Embodiment 1, except that its formula composition is as follows: 43.27 kg of corn, 24.67 kg of soybean meal, 2.79 kg of soybean oil, 0.23 kg of salt, 0.15 kg of methionine, 0.11 kg of lysine, 0.03 kg of threonine, 0.01 kg of tryptophan, 2.625 kg of calcium and phosphorus components, and 0.9 kg of electrolyte components.

[0069] Among them, the calcium and phosphorus components are composed of 1.1400kg of calcium hydrogen phosphate, 0.8625kg of stone powder and 0.6225kg of gravel; the electrolyte component is composed of 350.69g of sodium bicarbonate and 549.31g of zeolite powder.

[0070] In this embodiment, the calcium content is 0.9%, the non-phytate phosphorus content is 0.45%, and the DEB value is 250.

[0071] Example 9 The present embodiment provides a low-protein feed for broiler chickens, which is basically the same as that of Embodiment 1, except that its formula composition is as follows: 43.27 kg of corn, 24.67 kg of soybean meal, 2.79 kg of soybean oil, 0.23 kg of salt, 0.15 kg of methionine, 0.11 kg of lysine, 0.03 kg of threonine, 0.01 kg of tryptophan, 2.625 kg of calcium and phosphorus components, and 0.9 kg of electrolyte components.

[0072] Among them, the calcium and phosphorus components are composed of 1.1400kg of calcium hydrogen phosphate, 0.8625kg of stone powder and 0.6225kg of gravel; the electrolyte component is composed of 667.36g of sodium bicarbonate and 232.64g of zeolite powder.

[0073] In this embodiment, the calcium content is 0.9%, the non-phytate phosphorus content is 0.45%, and the DEB value is 300.

[0074] Example 10 The present embodiment provides a low-protein feed for broilers, whose formula composition is as follows: 49.422kg of corn, 25.384kg of soybean meal, 2.030kg of soybean oil, 0.24kg of salt, 0.132kg of methionine, 0.104kg of lysine, 0.024kg of threonine, 0.024kg of tryptophan, 1.328kg of calcium hydrogen phosphate, 0.896kg of stone powder, 0.132kg of trace ingredients (excluding copper, iron, manganese, and zinc) and 0.180kg of trace mineral elements (corn starch and copper, iron, manganese, and zinc components).

[0075] Among them, trace mineral elements include 0.6gCuSO4·5H2O, 6.49gFeSO4·H2O, 9.16gMnSO4.H2O, 16.52gZnSO4·H2O and 117.23g corn starch.

[0076] Table 2 Diet composition and nutritional level of Ephedra chickens aged 1-30 days (wt%)

[0077] In Table 2, trace ingredients involve Se, I, choline and Jingmu broiler multivitamin; trace ingredients added per kilogram of feed: vitamin A 6600 IU, vitamin D3 2880 IU, vitamin E 42 IU, vitamin K3 2.1 mg, vitamin B1 2.67 mg, vitamin B2 5.4 mg, vitamin B6 3.75 mg, vitamin B12 0.024 mg, calcium pantothenate 13.05 mg, niacinamide 42 mg, folic acid 1.32 mg, biotin 0.18 mg, choline 700 mg, iodine 0.35 mg, selenium 0.15 mg; the crude protein, calcium and phosphorus contents in the nutrients are measured values.

[0078] It can be seen from Table 2 that the crude protein content of the broiler low-protein feed provided in this embodiment is 18.98%, the calcium content is 0.86%, and the non-phytic acid phosphorus content is 0.41%. Through calculation, the DEB value in this embodiment is 210.78.

[0079] Embodiment 11 The present embodiment provides a low-protein feed for broilers, whose formula composition is as follows: 49.422kg of corn, 25.384kg of soybean meal, 2.030kg of soybean oil, 0.24kg of salt, 0.132kg of methionine, 0.104kg of lysine, 0.024kg of threonine, 0.024kg of tryptophan, 1.328kg of calcium hydrogen phosphate, 0.896kg of stone powder, 0.132kg of trace ingredients (excluding copper, iron, manganese, and zinc) and 0.180kg of starch and copper, iron, manganese, and zinc ingredients.

[0080] Among them, trace mineral elements include 1.0gCuSO4·5H2O, 12.97gFeSO4·H2O, 18.32gMnSO4.H2O, 13.77gZnSO4·H2O and 103.94g corn starch.

[0081] It can be seen from Table 2 that the crude protein content of the broiler low-protein feed provided in this embodiment is 18.98%, the calcium content is 0.86%, and the non-phytic acid phosphorus content is 0.41%. Through calculation, the DEB value in this embodiment is 210.78.

[0082] Example 12 The present embodiment provides a low-protein feed for broilers, whose formula composition is as follows: 49.422kg of corn, 25.384kg of soybean meal, 2.030kg of soybean oil, 0.24kg of salt, 0.132kg of methionine, 0.104kg of lysine, 0.024kg of threonine, 0.024kg of tryptophan, 1.328kg of calcium hydrogen phosphate, 0.896kg of stone powder, 0.132kg of trace ingredients (excluding copper, iron, manganese, and zinc) and 0.180kg of starch and copper, iron, manganese, and zinc ingredients.

[0083] Among them, trace mineral elements include 1.4gCuSO4·5H2O, 19.46gFeSO4·H2O, 6.11gMnSO4.H2O, 11.02gZnSO4·H2O and 112.02g corn starch.

[0084] It can be seen from Table 2 that the crude protein content of the broiler low-protein feed provided in this embodiment is 18.98%, the calcium content is 0.86%, and the non-phytic acid phosphorus content is 0.41%. Through calculation, the DEB value in this embodiment is 210.78.

[0085] Example 13 The present embodiment provides a low-protein feed for broilers, whose formula composition is as follows: 49.422kg of corn, 25.384kg of soybean meal, 2.030kg of soybean oil, 0.24kg of salt, 0.132kg of methionine, 0.104kg of lysine, 0.024kg of threonine, 0.024kg of tryptophan, 1.328kg of calcium hydrogen phosphate, 0.896kg of stone powder, 0.132kg of trace ingredients (excluding copper, iron, manganese, and zinc) and 0.180kg of starch and copper, iron, manganese, and zinc ingredients.

[0086] Among them, trace mineral elements include 1.79gCuSO4·5H2O, 3.24gFeSO4·H2O, 15.26gMnSO4.H2O, 8.26gZnSO4·H2O and 121.44g corn starch.

[0087] It can be seen from Table 2 that the crude protein content of the broiler low-protein feed provided in this embodiment is 18.98%, the calcium content is 0.86%, and the non-phytic acid phosphorus content is 0.41%. Through calculation, the DEB value in this embodiment is 210.78.

[0088] Embodiment 14 The present embodiment provides a low-protein feed for broiler chickens, which is basically the same as that of Embodiment 10, except that its formula composition is as follows: 30.796 kg of corn, 15.817 kg of soybean meal, 1.265 kg of soybean oil, 0.150 kg of salt, 0.082 kg of methionine, 0.065 kg of lysine, 0.015 kg of threonine, 0.015 kg of tryptophan, 0.828 kg of calcium hydrogen phosphate, 0.558 kg of stone powder, 0.110 kg of trace ingredients and 0.150 kg of trace mineral elements.

[0089] Among them, trace mineral elements include 1.79gCuSO4·5H2O, 3.24gFeSO4·H2O, 15.26gMnSO4.H2O, 8.26gZnSO4·H2O and 121.45g corn starch.

[0090] The broiler low-protein feed provided in this embodiment has a crude protein content of 18.98%, a calcium content of 0.86%, and a non-phytic acid phosphorus content of 0.41%. Through calculation, the DEB value in this embodiment is 210.78.

[0091] Embodiment 15 The present embodiment provides a low-protein feed for broilers, whose formula composition is as follows: 49.422kg of corn, 25.384kg of soybean meal, 2.030kg of soybean oil, 0.24kg of salt, 0.132kg of methionine, 0.104kg of lysine, 0.024kg of threonine, 0.024kg of tryptophan, 1.328kg of calcium hydrogen phosphate, 0.896kg of stone powder, 0.132kg of trace ingredients (excluding copper, iron, manganese, and zinc) and 0.180kg of starch and copper, iron, manganese, and zinc ingredients.

[0092] Among them, trace mineral elements include 2.19gCuSO4·5H2O, 9.73gFeSO4·H2O, 3.05gMnSO4.H2O, 5.51gZnSO4·H2O and 129.52g corn starch.

[0093] It can be seen from Table 2 that the crude protein content of the broiler low-protein feed provided in this embodiment is 18.98%, the calcium content is 0.86%, and the non-phytic acid phosphorus content is 0.41%. Through calculation, the DEB value in this embodiment is 210.78.

[0094] Test Example 1 Test subjects: low-protein feed for broiler chickens provided in Examples 1-9.

[0095] Experimental method: 324 one-day-old male ephedra chickens (i.e. yellow-feathered broilers) were randomly divided into 9 treatment groups according to their average body weight, and were fed with the broiler low-protein feed provided in Examples 1-9, with 6 replicate cages in each group and 6 chickens in each replicate cage, and were raised in stainless steel plastic-coated chicken cages for a test period of 30 days. The chickens were free to eat and drink tap water, with 24 h constant light, and normal immunization. During the test, the health status of the chickens was observed and recorded daily. If any chickens became ill or died, they were immediately dissected, the pathological cause of death was observed and analyzed, and the materials were aggregated. At the end of each week, 15% of the total number of chickens were sampled and weighed to ensure that the weight of the chickens met the standard.

[0096] (1) Observe the growth, development, morbidity and mortality of chickens every day, and record the number of deaths and culls. Weigh the empty-stomach weight of chickens in duplicate cages before slaughtering on the morning of the next day (31 years old) and calculate the average daily weight gain, daily feed intake and feed / weight gain ratio.

[0097] (2) On the morning of 31 days of age, two experimental chickens with a body weight close to the average were selected from each replicate cage and killed. Part of the left pectoral muscle was taken and placed in a sample bag and frozen at -20°C for the determination of muscle protein content. The left tibia was taken and placed in a sample bag and frozen at -20°C for the determination of tibia mineral content, bone density, bone compressive strength, tibia ash content, ash Ca and P content.

[0098] (3) After the 31-day-old slaughter experiment, two chickens with a body weight close to the average were kept in each replicate cage of the nine treatment groups to start the metabolic experiment. Feeding was resumed at 15:00 in the afternoon, and feces were collected at the same time. Feeding was stopped after 55 hours, and the feed consumption was calculated; feces were collected for 17 hours (a total of 72 hours). During the fasting period, the chickens had free access to water and supplemented with glucose saline (5% glucose solution, NaCl concentration of 0.9%).

[0099] (4) Before collecting feces, first remove feathers and obvious debris from the feces tray. If there is any spilled feed, weigh it and deduct it from the feed consumption. After collecting the feces, add 10 mL of 10% hydrochloric acid for every 100 g of fresh feces to avoid the loss of ammonia nitrogen in the feces. Then put the feces into a covered container; collect feces every day and store them in a refrigerator below 4°C. After 3 days of collection, weigh them, dry all the feces at 55-65°C, crush them, pass them through a 40-mesh analysis sieve, grind the sieved material and mix them evenly, take an appropriate amount of sample and put it into a self-sealing bag; store them in a refrigerator at 0-4°C to determine the N, calcium and phosphorus content in the feces.

[0100] Results test: (1) Determine the nitrogen (N) content in feces and the crude protein content in muscle using the conventional Kjeldahl method (GB / T 6432-2018); (2) Determination of Ca content in tibia ash using plasma emission spectrometry; (3) Bone performance indicators: The compressive strength of the tibia was measured using a CMT4504 microcomputer-controlled electronic universal testing machine. The tibial bone density and bone mineral content were scanned using dual-energy X-ray absorptiometry (DEXA). The ash content of the tibia was measured in accordance with the national standard GB / T 6438-2007.

[0101] Test results: (1) The growth performance test results of Ephedra chickens aged 1 to 30 days are shown in Table 2.

[0102] Data analysis: The general linear model (GLM) program in SAS 9.1 system was used to perform two-way ANOVA on the obtained data; if the ANOVA was significant, the least significant difference (LSD) method was used to compare the significance of the differences between the means. P ≤ 0.05 was used as the significance test level. 0.05﹤P﹤0.10 indicated a significant trend. The analysis results are shown in Table 3.

[0103] Table 3 Effects of different dietary calcium, phosphorus and electrolyte levels on growth performance of Ephedra chickens aged 1-30 days (n=6)

[0104] In Table 3, the values ​​are the average of 6 replicates (n=6), the same as in the following table. Different letters in the shoulder (a and b) indicate significant differences between the two groups of data, the same as in the following table. NPP: non-phytate phosphorus; DEB: electrolyte balance (Na + +k + -cl - ), the same as in the table below.

[0105] As can be seen from Table 3, the use of the low-protein feed provided in Examples 1-9 of the present invention as feed to feed Ephedra chickens can effectively improve the growth performance of Ephedra chickens. In particular, the electrolyte balance value provided by the present invention can significantly improve the average body weight, average daily feed intake and average daily weight gain ( P <0.05), especially when the DEB value was 300.

[0106] (2) The test results of tibia characteristics of Ephedra chickens aged 1 to 30 days are shown in Table 4.

[0107] Table 4 Effects of different dietary calcium, phosphorus and electrolyte levels on tibia characteristics of Ephedra chickens aged 1-30 days (n=6)

[0108] As shown in Table 4, the low-protein feed provided by Examples 1-9 of the present invention can be used to feed Ephedra chickens, which can effectively improve the bone development of Ephedra chickens, mainly reflected in the tibia bone density and tibia ash content. In particular, when the calcium and non-phytate phosphorus levels are 0.9% and 0.45%, respectively, the tibia bone density and tibia ash content of yellow-feathered broilers can be significantly improved ( P <0.05).

[0109] When the calcium and non-phytate phosphorus levels were 0.9% and 0.45%, respectively, the tibia compressive strength of the treatment groups with electrolyte DEB values ​​of 250 and 300 was greater than that of the treatment group with electrolyte DEB value of 200.

[0110] When the calcium and non-phytate phosphorus levels were 0.9% and 0.45%, respectively, the P content in the tibia was significantly higher than that in the medium and low calcium and phosphorus treatment groups ( P <0.05).

[0111] The above results indicate that the low-protein feed provided by the present invention can effectively improve the bone development of yellow-feathered broilers.

[0112] (3) The nitrogen deposition and breast protein content of Ephedra chickens aged 1 to 30 days are shown in Table 5.

[0113] Table 5 Effects of different dietary calcium, phosphorus and electrolyte levels on breast protein content, nitrogen deposition and calcium and phosphorus excretion of Ephedra chickens aged 1-30 days (n=6)

[0114] As shown in Table 5, the interaction of dietary calcium, non-phytate phosphorus and electrolyte levels had a significant effect on N deposition in yellow-feathered broilers ( P <0.05, when the calcium and phosphorus levels were 0.9% and 0.45%, respectively, the N deposition in the treatment groups with DEB values ​​of 300 and 250 was significantly higher than that in the treatment group with DEB value of 200 ( P <0.05).

[0115] As shown in Table 5, the interaction of dietary calcium, non-phytate phosphorus and electrolyte levels had a significant effect on the crude protein content of yellow-feathered broiler breast meat ( P <0.05). When DEB levels were 200 and 300, calcium and phosphorus levels had no significant effect on the crude protein content of breast meat ( P <0.05), when the DEB level was 250, the crude protein content of breast meat decreased significantly with the increase of calcium and phosphorus levels ( P <0.05), calcium and phosphorus levels of 0.9%, 0.45%, and DEB value of 250 can significantly reduce the crude protein content of breast meat ( P <0.05).

[0116] As shown in Table 5, the interaction of dietary calcium, non-phytate phosphorus and electrolyte levels had no significant effect on the fecal calcium content of yellow-feathered broiler chickens ( P >0.05, but dietary calcium and phosphorus levels significantly affected fecal calcium content ( P <0.05), the higher the dietary calcium and phosphorus levels, the higher the fecal calcium content.

[0117] As shown in Table 5, the interaction of dietary calcium, non-phytate phosphorus and electrolyte levels had a significant effect on the phosphorus content of yellow-feathered broiler feces ( P <0.05, dietary calcium and phosphorus levels significantly affected fecal phosphorus content ( P <0.05, the higher the calcium and phosphorus levels in the diet, the higher the phosphorus content in the feces. When the calcium and phosphorus levels were 0.7% and 0.35%, respectively, the phosphorus content in the feces of the DEB 300 treatment group was significantly higher than that of the DEB 200 treatment group ( P <0.05), when the calcium and phosphorus levels were 0.8% and 0.4% respectively, the phosphorus content in feces of the treatment groups with DEB of 300 and 250 was significantly higher than that of the treatment group with DEB of 200 ( P <0.05).

[0118] The above results indicate that the low-protein feed provided by the present invention can more effectively improve the utilization of macro-mineral elements.

[0119] (4) The test results of calcium and phosphorus deposition and deposition rate in the tibia of Ephedra chickens aged 1 to 30 days are shown in Table 6.

[0120] Table 6 Effects of different dietary calcium, phosphorus and electrolyte levels on the amount and rate of calcium and phosphorus deposition in the tibia of 1-30-day-old Ephedra chickens (n=6)

[0121] As shown in Table 6, the interaction of dietary calcium, non-phytate phosphorus and electrolyte levels had a significant effect on the total amount of calcium deposition in the tibia of yellow-feathered broilers ( P <0.05, when the calcium and phosphorus levels were 0.9% and 0.45%, respectively, the N deposition in the treatment groups with DEB values ​​of 300 and 250 was significantly higher than that in the treatment group with DEB value of 200 ( P <0.05). The levels of dietary calcium and non-phytate phosphorus significantly affected the total phosphorus deposition in the tibia and the calcium-phosphorus deposition rate in the tibia. The total phosphorus deposition in the tibia of the treatment group with dietary calcium and non-phytate phosphorus levels of 0.9% and 0.45%, respectively, was significantly higher than that in the low calcium-phosphorus treatment group, but the calcium-phosphorus deposition rate in the tibia of the treatment group with dietary calcium and non-phytate phosphorus levels of 0.9% and 0.45%, respectively, was significantly lower than that in the medium and low calcium-phosphorus treatment groups.

[0122] The above results indicate that the low-protein feed provided by the present invention can effectively improve the deposition of calcium and phosphorus in the tibia of yellow-feathered broilers and improve the bone development of yellow-feathered broilers.

[0123] Test Example 2 Test subjects: low-protein feed for broiler chickens provided in Examples 10-15.

[0124] Experimental method: 216 newly hatched one-day-old medium-speed yellow-feathered broiler chicks were randomly divided into 6 treatment groups according to their average body weight. They were fed with the broiler low-protein feed provided in Examples 10-15, with 6 replicates in each group and 6 chickens in each replicate cage. They were raised in stainless steel plastic-coated chicken cages for a test period of 30 days. The chickens were free to eat and drink tap water, and the light was constant for 24 hours. The health status of the chickens was observed and recorded daily. At the end of each week, 15% of the total number of chickens were sampled and weighed to ensure that the weight of the chickens met the standard.

[0125] (1) Observation of growth performance indicators The growth, development, morbidity and mortality of the chickens were observed every day, and the number of deaths and cullings was recorded. The chickens were fed at 30 days of age and weighed on an empty stomach basis in duplicate cages before slaughter the next morning (31 years of age), and the average daily weight gain, daily feed intake and feed / weight gain ratio were calculated.

[0126] On the morning of 31 days of age, two experimental chickens with close to average body weight were selected from each replicate cage, slaughtered, and the hearts and livers were collected and stored at -20°C for the determination of Cu, Fe, Mn and Zn contents, total SOD activity, copper-zinc SOD activity, total antioxidant capacity, and malondialdehyde content.

[0127] test: (1) Dietary crude protein and element contents in diet and tissues The nitrogen content in feces was determined by the conventional Kjeldahl method (GB / T 6432-2018); the Cu, Fe, Mn and Zn contents in heart and liver were determined by plasma emission spectrometry.

[0128] (2) Tissue antioxidant indicators and key enzyme activities The total antioxidant capacity, total SOD activity, copper-zinc SOD activity, and malondialdehyde content of the heart and liver were determined using a kit produced in Nanjing.

[0129] Data Statistical Analysis: The experimental data are expressed as mean ± standard error. DPS (v 9.05) software was used to perform quadratic polynomial stepwise regression with replicates as the unit. The quadratic polynomial regression model is as follows: Y=β0+∑β i x i +∑β ii x i 2 +∑ⁿ i β ij x i x j i ≠ j; i, j = 1, 2, 3… Y is the predicted value, β0 is a constant, β i , β ii and β ij They are linear regression coefficient, quadratic regression coefficient and interaction regression coefficient, X i and X j is the independent variable. X1, X2, X3 and X4 represent the content of trace elements copper, iron, zinc and manganese in the feed. The coefficient of determination R 2 The degree of fit of the polynomial regression equation was determined, and P<0.05 was used as the criterion for the significance of each regression term.

[0130] Test results: (1) The growth performance test results of Ephedra chickens aged 1 to 30 days are shown in Table 7.

[0131] Table 7 Growth performance test results of Ephedra chickens aged 1-30 days

[0132] The quadratic polynomial regression equation was fitted with the data in Table 7, and the optimal solution analysis was performed. It can be seen that when the contents of copper, iron, zinc and manganese in the feed are 12.99, 120.00, 120.00 and 60.74 mg / kg respectively, the growth performance of yellow-feathered broilers aged 1-30 days can be effectively improved.

[0133] (2) The results of element deposition in the tissues of Ephedra chickens aged 1 to 30 days are shown in Table 8.

[0134] Table 8 Contents of Cu, Fe, Mn and Zn in heart and liver tissues (g / kg)

[0135] From the data in Table 8, a quadratic polynomial regression equation was fitted and the optimal solution analysis was performed. It can be seen that in low-protein diets, when the contents of copper, iron, zinc and manganese in the diets were 13, 84-120, 82-120 and 102-120 respectively, the deposition of copper, iron, manganese and zinc in the heart and liver can be effectively improved.

[0136] (3) The results of antioxidant indexes and key enzyme activities in the tissues of Ephedra chickens aged 1 to 30 days are shown in Table 9.

[0137] Table 9 Key enzyme activities of tissues

[0138] The data in Table 9 were fitted with a quadratic polynomial regression equation, and the optimal solution analysis showed that in low-protein diets, when the contents of copper, iron, zinc, and manganese in the diet were 8-13, 96-118, 83.20-120, and 21-120 mg / kg, respectively, the liver antioxidant capacity could be effectively improved. When the contents of copper, iron, zinc, and manganese in the diet were 3, 21-23, 119-120, and 90-120, respectively, the heart antioxidant capacity could be effectively improved.

[0139] In summary, the broiler low-protein feed provided by the present invention can meet the calcium, phosphorus and electrolyte requirements of yellow-feathered broilers, and can effectively improve growth performance, improve bone development, improve nitrogen deposition, improve the metabolic utilization and discharge of major mineral elements, and improve trace element deposition.

[0140] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A low-protein feed for broiler chickens, characterized in that: The invention comprises limiting amino acids, wherein the crude protein content is 18-20wt%, the calcium and non-phytic acid phosphorus contents are 0.7-1.0wt% and 0.3-0.6wt% respectively, and the electrolyte balance value is 200-300mmol / kg.

2. The broiler low-protein feed according to claim 1, characterized in that: The crude protein content is 18.5-19.5wt%, the calcium and non-phytic phosphorus contents are 0.8-0.9wt% and 0.4-0.5wt% respectively, and the electrolyte balance value is 250-300mmol / kg.

3. The low-protein feed according to claim 1 or 2, characterized in that: It also includes Cu, Fe, Mn and Zn with contents of 8-13, 95-120, 83-120 and 61-90 mg / kg respectively.

4. The low-protein feed according to any one of claims 1 to 3, characterized in that: The limiting amino acid is selected from at least one of methionine, lysine, tryptophan and threonine.

5. A low-protein feed for broiler chickens, characterized in that: Calculated by weight, it includes: 55-65 parts of corn, 30-35 parts of soybean meal, 2-4 parts of soybean oil, 0.2-0.4 parts of salt, 0.15-0.25 parts of methionine, 0.1-0.15 parts of lysine, 0.02-0.05 parts of threonine, 0.01-0.03 parts of tryptophan, 2-4 parts of calcium and phosphorus components, 1-1.5 parts of electrolyte components and / or 0.2-0.4 parts of trace mineral elements components, and 0.20-0.35 parts of trace ingredients.

6. The broiler low-protein feed according to claim 5, characterized in that: By weight, it includes 55-60 parts of corn, 30-35 parts of soybean meal, 3-4 parts of soybean oil, 0.2-0.4 parts of salt, 0.15-0.25 parts of methionine, 0.1-0.15 parts of lysine, 0.03-0.05 parts of threonine, 0.01-0.02 parts of tryptophan, 3-4 parts of calcium and phosphorus components, 1-1.5 parts of electrolyte components, and 0.25-0.35 parts of trace ingredients; preferably, the total amount is 100 parts.

7. The low-protein feed according to claim 6, characterized in that: The electrolyte component includes 1-1.2 parts of sodium bicarbonate and zeolite powder, and the feed includes 0.4-10 g / kg of the sodium bicarbonate based on the weight of the feed; And / or, the calcium-phosphorus components include 0.5-2 parts of calcium hydrogen phosphate, 0.5-1.5 parts of stone powder, and 0.8-1.6 parts of gravel.

8. The low-protein feed for broiler chickens according to claim 5, characterized in that: Calculated by weight, it includes 60-65 parts of corn, 30-35 parts of soybean meal, 2-3 parts of soybean oil, 0.25-0.35 parts of salt, 0.15-0.20 parts of methionine, 0.10-0.15 parts of lysine, 0.02-0.04 parts of threonine, 0.02-0.03 parts of tryptophan, 2-3 parts of calcium and phosphorus components, 0.20-0.25 parts of trace ingredients, and 0.2-0.4 parts of trace mineral elements; preferably, the total amount is 100 parts.

9. The low-protein feed according to claim 8, characterized in that: The trace mineral element components include copper sulfate, ferrous sulfate, manganese sulfate, zinc sulfate and corn starch. Based on the weight of the feed, the feed includes 0.01-0.06 g / kg of copper sulfate, 0.05-0.4 g / kg of ferrous sulfate, 0.05-0.4 g / kg of manganese sulfate, 0.05-0.4 g / kg of zinc sulfate and 0.20-0.26 parts of corn starch.

10. Use of the low-protein feed according to any one of claims 1 to 9 in yellow-feathered broilers; Preferably, the application includes using the broiler low-protein feed to improve at least one of growth performance, bone development, nitrogen deposition, metabolic utilization and discharge of macro-mineral elements and trace element deposition.