Composite nutrient with anti-aging function

Abstract

The invention discloses a compound nutrient with an anti-aging function, which comprises the following components in parts by mass: 15-25 parts of NMN powder, 10-20 parts of betaine, 5-15 parts of green tea extract, 5-15 parts of astragalus extract, 3-8 parts of burdock extract, 3-8 parts of sialic acid, 1-5 parts of ginseng root extract and 1-3 parts of silybum marianum extract. Various anti-aging active ingredients such as the NMN, the radix astragali extract, the ginseng root extract and the silybum marianum extract are combined, and the anti-aging effect is achieved through the synergistic effect. The characteristics and the action mechanism of each component are fully considered for research, development and preparation of the medicine, so that the safety, the stability and the effectiveness of the medicine are ensured, the formula proportion and component selection are optimized, the absorption rate of the human body to the nutritional components is increased, the synergistic effect of all the components is enhanced, and the nutritional supplement which is broad in spectrum and efficient in absorption is provided. The defects of existing products on the market are overcome.

Description

Technical Field

[0001] The invention belongs to the field of food and relates to a composite nutrient, in particular to a composite nutrient with anti-aging function. Background Art

[0002] Aging is an irreversible growth process of organisms that occurs spontaneously over time. It is a complex natural phenomenon that occurs due to different destructive stimuli and manifests itself as structural and functional decline, and reduced adaptability and resistance. In physiology, aging is considered to be an individual developmental history that begins from the fertilized egg and continues until old age. Aging has pleiotropic effects on development, tissue aging and regeneration, inflammation, wound healing, and tumor suppression, including DNA damage, telomere shortening, and dysfunction or carcinogenic stress. How to scientifically fight aging and delay the occurrence and development of aging-related diseases has become a hot topic of concern for many people. Aging itself is not terrible. What is terrible is that degenerative changes will occur in various systems during the aging process, and together with disease-specific factors, they will cause aging-related diseases, such as heart disease, vascular atherosclerosis, osteoporosis, and degenerative lesions of the central nervous system.

[0003] β-Nicotinamide mononucleotide, abbreviated as NMN, has a molecular formula of C 11 H 15 N2O8P, molecular weight is 334.22. In recent years, many studies have shown that NMN has potential application prospects in the field of anti-aging. NMN exists naturally in the human body and has significant physiological benefits. As a coenzyme I (NAD + ) in the biosynthetic pathway of NAD + Although NMN can be obtained from a variety of ingredients such as broccoli, cabbage, cucumber, edamame, avocado, etc., its content is very small and cannot be used as an effective supplement source. In the human body, the physiological function of NMN is mainly through increasing NAD + NMN helps maintain telomere length, activate stem cells, activate DNA repair enzymes, repair damaged DNA, etc., thereby delaying cell aging and overall body aging in many ways. As a new type of nutritional supplement, NMN plays an increasingly positive role in enhancing body vitality, improving sleep, anti-aging, and promoting physical health for middle-aged and elderly people with weak nutrient absorption ability, decreased physical function, and low NMN synthesis levels.

[0004] Many NMN anti-aging nutrients currently on the market contain only a single key ingredient without considering its interaction with other ingredients, which may limit its effectiveness. Even if supplements contain beneficial ingredients, their effectiveness is often limited by low bioavailability in the human body. The absorption and utilization rates of some key nutrients are low in traditional supplements, and there is still room for improvement, especially in the formulation of supplements and cellular absorption efficiency. Summary of the invention

[0005] In response to the problem that the absorption and utilization rate of the above-mentioned NMN anti-aging nutrient is low in traditional supplements, the present invention provides a composite nutrient with anti-aging function, which combines NMN, astragalus extract, ginseng root extract, milk thistle extract and other anti-aging active ingredients to exert anti-aging effects through synergistic effects.

[0006] In order to achieve the above-mentioned purpose, the present invention provides a composite nutrient with anti-aging function, which comprises the following components by mass: 15-25 parts of NMN powder, 10-20 parts of betaine, 5-15 parts of green tea extract, 5-15 parts of astragalus extract, 3-8 parts of burdock extract, 3-8 parts of sialic acid, 1-5 parts of ginseng root extract, and 1-3 parts of milk thistle extract.

[0007] NMN is nicotinamide mononucleotide, a derivative of vitamin B3, a substance naturally present in the human body, with a molecular formula of C 11 H 15 N2O8P.

[0008] The composite nutrient of the present invention contains NMN powder, which has the anti-oxidation, anti-inflammatory, anti-stress ability, body function and anti-aging effects of maintaining a youthful state. It plays a key role in the anti-aging process. It can increase the NAD in the body. + The content of NMN can repair damaged DNA, help maintain telomere length, activate stem cells, etc., and regulate chronic inflammation, oxidative stress and other aging markers. Adding NMN powder can improve energy metabolism, increase cell capacity, and repair damaged cells to delay human aging.

[0009] Betaine can target antioxidant effects and reduce homocysteine ​​levels, and together with NMN, it can reduce the damage of oxidative stress to cells, affect signaling pathways such as Nrf2, AMPK, and NF-κB, and delay cell aging.

[0010] Green tea extract can work with NMN powder to improve the body's metabolic environment, synergistically affect the Nrf2, AMPK, and p53 signaling pathways, jointly target chronic inflammation, DNA damage, etc., and enhance cell repair capabilities.

[0011] Ginseng root extract is a Chinese herbal medicine extract. The main bioactive ingredient is ginsenoside, which has no drug resistance and has anti-inflammatory, anti-fatigue, antioxidant, and anti-cancer effects. It can enhance the body's immune function, affect the level of stress hormones, and enhance energy metabolism. Ginseng root extract has the effects of improving physical strength, immunity, and cognitive function. It coordinates with NMN to enhance the body's immunity and repair ability, and affects the AMPK, NF-κB, and SIRT1 signaling pathways. Astragalus extract has the effects of enhancing the immune system, resisting fatigue and stress, targeting aging markers such as cell apoptosis, autophagy and stem cell proliferation and differentiation, maintaining telomerase activity with NMN, affecting AMPK and p53 signaling pathways, and slowing down cell aging.

[0012] Burdock extract can target aging markers such as mitochondrial function, chronic anti-inflammation, antioxidant cell autophagy, and protein homeostasis regulation, and synergize with NMN to enhance antioxidant and anti-inflammatory capabilities. It can jointly affect the AMPK and NF-κB signaling pathways to promote cell health.

[0013] Sialic acid has positive effects on neuroprotection and immunoregulation, and synergizes with NMN to regulate the immune system, affecting the AMPK, SIRT1, and PI3K / Akt signaling pathways to maintain nerve cell function.

[0014] Milk thistle extract is extracted from the seeds of milk thistle. It is a powerful antioxidant that can protect the liver from damage by free radicals and toxins and help remove harmful substances from the body. When used in skin care, it can slow down the aging process and treat skin conditions. Milk thistle extract protects the liver from damage by free radicals and toxins, and synergizes with NMN to protect liver cells, affecting the AMPK, Nrf2-ARE, and NF-κB signaling pathways.

[0015] The above eight substances are mixed according to specific weight proportions, and the components work synergistically with each other to fight against the aging of the body from many aspects.

[0016] like Figure 1As shown, NMN has synergistic effects with betaine, green tea extract, astragalus extract, burdock extract, sialic acid, ginseng root extract and milk thistle extract in many aspects. In terms of metabolism, NMN and betaine jointly maintain the balance of intracellular metabolism. NMN provides energy support, and betaine regulates methylation to affect the activity of metabolism-related enzymes; it synergizes with astragalus extract to improve the efficiency of cell energy metabolism and promote the overall metabolism of the body. In terms of antioxidant, NMN is combined with green tea extract. The former ensures the stability of cell energy and enhances its own antioxidant capacity, while the latter's tea polyphenols directly remove free radicals and enhance the antioxidant effect. In immune regulation, NMN and ginseng root extract and astragalus extract jointly supply energy to immune cells and regulate their functions, enhancing the body's immunity; sialic acid cooperates with NMN in the immune recognition link to improve immune regulation. In the field of liver protection, NMN and milk thistle extract synergize in maintaining liver cell energy and promoting regeneration through antioxidant and anti-inflammatory effects. In terms of intestinal health, NMN and burdock extract work together to maintain the function of intestinal epithelial cells and regulate the balance of flora to ensure intestinal health.

[0017] In the multi-faceted anti-aging process, betaine, green tea extract, astragalus extract, burdock extract, sialic acid, ginseng root extract, and milk thistle extract showed significant synergistic effects. In terms of antioxidants, the above ingredients all work synergistically to enhance antioxidant effects. In terms of anti-inflammatory, astragalus extract, green tea extract, milk thistle extract, and ginseng root extract jointly enhance anti-inflammatory effects. Astragalus extract, milk thistle extract, and ginseng root extract can jointly maintain telomere length and telomerase activity, and milk thistle extract and ginseng root extract can also jointly participate in DNA repair. In the field of immunomodulation, burdock extract, green tea extract, and astragalus extract enhance immune cell activity, milk thistle extract and ginseng root extract regulate the balance of the immune system, and the five work together to enhance the body's immune function. In terms of the nervous system, sialic acid helps nerve cell development and function maintenance, and ginseng root extract promotes nerve cell growth and signal transmission, which is beneficial to improving cognition. In terms of basal metabolism, betaine extract regulates intestinal flora and synergizes with green tea extract to improve basal metabolism. These extracts work synergistically from different angles and multiple links to promote the health and balance of the body.

[0018] Preferably, the complex nutrients include the following components by mass: 18 to 23 parts of NMN powder, 12 to 18 parts of betaine, 8 to 12 parts of green tea extract, 8 to 12 parts of astragalus extract, 4 to 6 parts of burdock extract, 4 to 6 parts of sialic acid, 2 to 4 parts of ginseng root extract, and 1 to 2 parts of milk thistle extract.

[0019] Further preferably, the complex nutrients include the following components by mass: 20 parts of NMN powder, 15 parts of betaine, 10 parts of green tea extract, 10 parts of astragalus extract, 5 parts of burdock extract, 5 parts of sialic acid, 3 parts of ginseng root extract, and 2 parts of milk thistle extract.

[0020] Preferably, the green tea extract is EGCG.

[0021] For easy administration, the above-mentioned composite nutrient can be made into tablets, which, based on the above-mentioned technical solution, further include the following components by mass: 5-10 parts of dextrin, 1-3 parts of microcrystalline cellulose, 0.5-2 parts of magnesium stearate, and 0.5-2 parts of silicon dioxide.

[0022] Dextrin is a polysaccharide that can be used as a filler, stabilizer or thickener to improve taste, help form tablets, and keep ingredients evenly distributed; microcrystalline cellulose, as a filler, increases the volume of the tablet and helps other ingredients disperse evenly; magnesium stearate is used as a lubricant in tablets to help prevent the finished product from adhering to manufacturing equipment and ensure the free flow of tablets during the pressing process; silicon dioxide mainly acts as a flow aid and anti-caking agent in complex nutrients, preventing ingredients from agglomerating during storage and ensuring the fluidity of particles.

[0023] Preferably, the complex nutrient further comprises the following components by mass: 6 parts of dextrin, 2 parts of microcrystalline cellulose, 1 part of magnesium stearate, and 1 part of silicon dioxide.

[0024] Preferably, the extraction process of betaine is as follows: adding 5 to 10 times the amount (v / w) of 70% to 90% ethanol solution as a solvent to dried and crushed beetroot and amaranth, soaking at room temperature for 12 to 24 hours, then stirring continuously at 40 to 60° C. for 4 to 8 hours, filtering while hot, distilling the filtrate at 60 to 80° C., recovering ethanol, purifying the remaining liquid through a silica gel column, and finally drying at 40 to 50° C. to obtain betaine powder; The extraction process of green tea extract is as follows: adding 8 to 12 times the amount (v / w) of water as a solvent to dried and crushed green tea leaves, soaking for 1 to 2 hours, stirring continuously at 80 to 100° C. for 3 to 5 hours, filtering, concentrating the filtrate to 1 / 3 to 1 / 2 of the original volume at 60 to 70° C., purifying with a macroporous adsorption resin, and drying at 50 to 60° C. to obtain green tea extract powder; The extraction process of the astragalus extract is as follows: adding 6 to 10 times (v / w) of a 60% to 80% methanol solution as a solvent to the dried and crushed astragalus, soaking for 10 to 18 hours, stirring continuously at 50 to 70° C. for 5 to 8 hours, filtering, concentrating the filtrate at 50 to 60° C., purifying with a polyamide column, drying at 40 to 50° C., and sieving with an 80 to 100 mesh sieve to obtain an astragalus extract powder; The extraction process of burdock extract is as follows: adding 4 to 8 times (v / w) of 50% to 70% acetone solution as a solvent to dried and crushed fresh burdock root, soaking at 30 to 50°C for 6 to 12 hours, heating to 60 to 80°C and filtering, concentrating the filtrate at 40 to 50°C, decolorizing and purifying with activated carbon, drying at 40 to 50°C, and sieving with a 60 to 80 mesh sieve to obtain burdock extract powder; The extraction process of ginseng root extract is as follows: adding 7 to 11 times (v / w) of 75% to 95% ethanol solution as a solvent to dried and crushed ginseng roots, soaking for 8 to 16 hours, and then performing reflux extraction at a reflux temperature of 70 to 85° C. for 3 to 5 hours, filtering, concentrating the filtrate at 60 to 70° C., purifying with an alumina column, and drying at 50 to 60° C. to obtain ginseng root extract powder; The extraction process of milk thistle extract is as follows: add 6 to 10 times the amount (v / w) of 80% to 95% ethanol solution as a solvent to the dried and crushed milk thistle seeds, soak for 10 to 18 hours, reflux extraction, the reflux temperature is 75 to 85°C, the reflux time is 2 to 4 hours, filter, concentrate the filtrate at 60 to 70°C, and dry at 50 to 60°C to obtain milk thistle extract powder.

[0025] Through the above technical solution, the present invention achieves the following beneficial effects: The present invention combines NMN, astragalus extract, ginseng root extract, milk thistle extract and other anti-aging active ingredients to exert anti-aging effects through synergistic effects. The research and development and preparation of the drug will fully consider the characteristics and mechanism of action of each ingredient to ensure its safety, stability and effectiveness. The present invention optimizes the formula ratio and ingredient selection, improves the absorption rate of nutrients by the human body, and enhances the synergistic effect between the ingredients to provide a broad-spectrum, highly efficient absorption nutritional supplement to make up for the shortcomings of existing products on the market. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 It is a schematic diagram of the synergistic effect of the pharmacological components of the present invention. DETAILED DESCRIPTION

[0027] The specific implementation of the present invention is described in detail below in conjunction with the examples. It should be understood that the specific implementation described herein is only used to illustrate and explain the present invention, and is not used to limit the present invention.

[0028] In the following examples, the extraction process of betaine is as follows: 5 times the amount (v / w) of 80% ethanol solution is added as a solvent to dried and crushed beetroot and amaranth, soaked at room temperature for 12 hours, then continuously stirred at 50°C for 58 hours, filtered while hot, distilled the filtrate at 80°C, recovered ethanol, and the remaining liquid was purified by a silica gel column, and finally dried at 50°C to obtain betaine powder; The extraction process of green tea extract is as follows: add 10 times the amount (v / w) of water as a solvent to dried and crushed green tea leaves, soak for 1.5 hours, stir continuously at 100°C for 4 hours, filter, concentrate the filtrate at 60°C to 1 / 3~1 / 2 of the original volume, purify with a macroporous adsorption resin, and dry at 60°C to obtain green tea extract powder; The extraction process of the astragalus extract is as follows: add 10 times the amount (v / w) of 60% methanol solution as a solvent to the dried and crushed astragalus, soak for 12 hours, stir continuously at 70°C for 5 hours, filter, concentrate the filtrate at 50°C, purify with a polyamide column, dry at 50°C, and sieve with a 80-100 mesh sieve to obtain the astragalus extract powder; The extraction process of burdock extract is as follows: add 5 times the amount (v / w) of 70% acetone solution as a solvent to the dried and crushed fresh burdock root, soak at 50°C for 6 hours, heat to 80°C and filter, concentrate the filtrate at 50°C, decolorize and purify with activated carbon, dry at 50°C, and sieve with a 60-80 mesh sieve to obtain burdock extract powder; The extraction process of ginseng root extract is as follows: add 10 times the amount (v / w) of 75% ethanol solution as a solvent to the dried and crushed ginseng root, soak for 12 hours, then perform reflux extraction, the reflux temperature is 80°C, the reflux time is 4 hours, filter, concentrate the filtrate at 70°C, purify it with an alumina column, and dry it at 60°C to obtain ginseng root extract powder; The extraction process of milk thistle extract is as follows: add 8 times the amount (v / w) of 90% ethanol solution as a solvent to the dried and crushed milk thistle seeds, soak for 12 hours, reflux extraction, the reflux temperature is 80°C, the reflux time is 3 hours, filter, concentrate the filtrate at 60°C, and dry at 60°C to obtain milk thistle extract powder.

[0029] Dextrin was purchased from Shandong Liaocheng Ahua Pharmaceutical Co., Ltd. The specification was pharmaceutical excipient grade, and the DE value was 10-20.

[0030] Microcrystalline cellulose was purchased from Anhui Shanhe Pharmaceutical Excipients Co., Ltd. The specification was PH101 model, with a particle size of about 50 μm, which met the pharmaceutical standards.

[0031] Magnesium stearate was purchased from Huzhou Zhanwang Pharmaceutical Co., Ltd. The specification was pharmaceutical grade and the magnesium oxide content was 6.5%~7.5%.

[0032] Silicon dioxide was purchased from Evonik Industries, Inc., and was food grade, fumed silicon dioxide, with a particle size of about 10-20 nm. Table 1 shows the ingredients and dosages of the examples and comparative examples.

[0033] Table 1 Composition and dosage of Examples and Comparative Examples (unit: g)

[0034] The components in the above examples and comparative examples are mixed uniformly to obtain a composite nutrient.

[0035] Experimental Example 1 Anti-aging effect of compound nutrients on RS fibroblasts in vitro The cells were divided into two groups, one group was the control group, which continued to be cultured in standard culture medium; the other group was the compound nutrient group, in which the compound nutrients of the above embodiments and comparative examples were added to the cell culture medium to a final concentration of 500 μM. Cell morphological changes were observed and recorded regularly. SA-β-gal activity was measured by a kit to evaluate the aging state of cells. The telomerase activity of each group was analyzed using a kit to analyze the effect of compound nutrients on telomere maintenance.

[0036] The SA-β-gal activity in the 2nd, 9th and 15th generation cells was determined by the SA-β-gal kit. The results showed that the SA-β-gal activity in RS fibroblasts treated with the compound nutrients was significantly weakened compared with the control group (Table 2). In the 2nd generation cells, the percentage of SA-β-gal active cells in the control group was 3.3%, while the percentage of SA-β-gal active cells in the compound nutrient group embodiments 1-5 was reduced to 2.5%, 2.1%, 2.4%, 2.3% and 2.4% respectively, and the percentage of comparative examples 1-15 fluctuated between 3.0% and 3.8%; the activity of the 9th generation cell control group reached 4.5%, and the corresponding percentages of the compound nutrient group embodiments were 3.2%, 2.7%, 3.3%, 2.8% and 3.1%, and the comparative percentage was 4.1%-4.8%; the percentage of SA-β-gal active cells in the 15th generation cell control group rose to 11.2%, and the percentage of SA-β-gal active cells in the compound nutrient group embodiments was reduced to 7.1%, 5.0%, 6.8%, 5.3% and 6.6%, and the comparative percentage was 9.5%-10.9%. This result strongly suggests that complex nutrients can significantly delay the process of cell aging and effectively maintain cells in a healthy and vibrant state, providing key cellular-level evidence for its anti-aging effect.

[0037] The telomerase activity of the 2nd, 9th, and 15th generations of cells was measured using an ELISA kit. The data showed that in each generation of RS fibroblasts, the telomerase activity in the complex nutrient treatment group was significantly increased compared with the control group (Table 3). Taking the second generation cells as an example, the telomerase activity of the control group (OD 450% P2 normal control group) was set to 1.00, and the compound nutrient group examples 1-5 were increased to 1.25, 1.25, 1.22, 1.21, 1.23, respectively, and the comparative examples 1-15 were between 1.02-1.08; the 9th generation cell control group was 0.81, and the compound nutrient group examples reached 1.04, 1.03, 1.04, 1.00, 1.07, and the comparative examples were 0.83-0.89; the 15th generation cell control group dropped to 0.72, and the compound nutrient group examples were maintained at 0.98, 0.92, 0.89, 0.90, 0.95, and the comparative examples were 0.81-0.87. This result further confirms that compound nutrients play an important role in maintaining telomerase activity and delaying cell aging, and deepens the understanding of its anti-aging effect from the molecular mechanism level.

[0038] Table 2 Results of SA-β-gal activity determination of various examples and comparative examples in cell experiments

[0039] Table 3 Telomerase activity test results of various examples and comparative examples in cell experiments

[0040] Experimental Example 2: Effect of Compound Factors on Alleviating Aging in Animal Aging Models 132 SD rats were selected and randomly divided into 22 groups, 6 rats in each group, including the control group: fully fed with commercial feed every day; the aging group: subcutaneously injected with 5% D-galactose solution 400mg / kg﹒ bw in the back of the neck of the rats, once at 9:00 am every day, for 56 consecutive days to establish an animal aging model; the compound nutrient group: fully fed with commercial feed containing 10% of the compound nutrients of the comparative example and the embodiment every day; the aging + compound nutrient group: subcutaneously injected with 5% D-galactose solution in the back of the neck every day, and fed with commercial feed containing 10% of the old compound nutrients of the comparative example and the embodiment. During the modeling period, the dynamic changes of the survival status and biological behavior of the rats were observed and recorded, and their body weight was weighed regularly. The mice were observed after the experiment.

[0041] The results of the GSH-Px test kit showed (Table 4) that in the liver tissue, the GSH-Px activity of the aging group was 230.46±9.77U / mg protein, which was significantly lower than that of the normal control group (267.28±13.22U / mg protein); while in the aging + compound nutrient group, the GSH-Px activities corresponding to Examples 1 to 5 were 256.64±15.30U / mg protein, 267.10±14.66U / mg protein, and 263 .09±13.84U / mg protein, 270.33±16.09U / mg protein, and 267.04±9.85U / mg protein, which were significantly increased compared with the aging group. The comparison groups 1 to 15 were between 231.21 and 241.24U / mg protein. Although higher than the aging group, the difference was not significant, indicating that the compound nutrients can effectively regulate the antioxidant enzyme system of rat liver, alleviate oxidative stress damage caused by aging, and enhance the liver's antioxidant defense ability.

[0042] The SOD test kit test data showed (Table 5) that the SOD activity of the aging group in liver tissue was 199.78±15.66NU / mg protein, which was much lower than 250.37±7.36NU / mg protein of the normal control group; in the aging+complex nutrient group, the SOD activities of Examples 1 to 5 were 239.12±13.89NU / mg protein, 238.91±15.02NU / mg protein, 234.90±14.66NU / mg protein, 241.49±14.92NU / mg protein, and 246.10±13.39NU / mg protein, respectively, which were significantly improved compared with the aging group. The comparative examples 1 to 15 were in the range of 199.58~215.29NU / mg protein, which was higher than that of the aging group, but the difference was not obvious, further confirming that the complex nutrients can enhance the activity of the body's antioxidant enzymes, reduce oxidative damage, and delay the aging process.

[0043] The MDA test kit detected (Table 6) that the MDA content in the liver tissue of the aging group was 2.51±0.84nmol / mg, which was significantly higher than 1.88±0.79nmol / mg of the normal control group; in the aging + compound nutrient group, the MDA contents of Examples 1 to 5 were 2.12±0.66nmol / mg, 1.94±0.70nmol / mg, 2.07±0.57nmol / mg, 2.00±0.34nmol / mg, and 1.99±0.31nmol / mg, respectively, which were significantly lower than those of the aging group. The comparative examples 1 to 15 were in the range of 2.45~2.53nmol / mg, which was not significantly different from that of the aging group, indicating that the compound nutrients can effectively inhibit lipid peroxidation, reduce the level of oxidative stress, protect cells from oxidative damage, and play a significant anti-aging effect.

[0044] NAD +The results of the NADH kit test showed (Table 7) that in the liver tissue, the NAD + The content was 318.26±27.9U / mg protein, which was significantly lower than 453.05±36.2U / mg protein in the normal control group; while in the aging + compound nutrient group, the NAD + The contents were 505.41±31.1U / mg protein, 523.72±31.7U / mg protein, 518.85±32.3U / mg protein, 520.14±32.9U / mg protein, and 519.20±33.5U / mg protein, respectively, which were significantly increased compared with the aging group. In addition, compared with the control group, the liver NAD + The level also increased significantly, which fully demonstrated that compound nutrient treatment can significantly improve the body's NAD + Metabolism, activate cell energy metabolism pathways, enhance cell vitality, and fight against the aging process.

[0045] The results of DNA damage test kit showed (Table 8) that the DNA damage level (%DNA incomettail) in the liver tissue of the aging group was 18.89±1.57, which was much higher than 12.87±1.22 in the normal control group; in the aging+complex nutrient group, the DNA damage levels of Examples 1 to 5 were 13.77±1.17, 12.53±1.23, 13.93±1.09, 13.24±1.26, and 13.61±1.14, respectively, which were significantly reduced compared with the aging group, and the comparison examples 1 to 15 were between 15.95 and 17.41, which was not significantly different from the aging group. At the same time, the composite nutrient alone treatment group (complex nutrient group) also showed a significant downward trend compared with the control group, which strongly proved that composite nutrients can effectively resist DNA damage induced by D-galactose, maintain genome stability, and delay the aging process.

[0046] The results of ELISA kit detection of telomerase activity in rat liver showed (Table 9) that the telomerase activity in the aging group in liver tissue was 1.35±0.05U / mg protein, which was lower than 1.99±0.07U / mg protein in the normal control group; in the aging+complex nutrient group, the telomerase activities of Examples 1 to 5 were 1.75±0.23U / mg protein, 1.78±0.32U / mg protein, 1.80±0.15U / mg protein, 1.72±0.09U / mg protein, and 1.75±0.14U / mg protein, respectively, which were significantly higher than those in the aging group, and the telomerase activities of Comparative Examples 1 to 15 were between 1.35 and 1.60U / mg protein, which were not significantly different from those in the aging group. This result once again verifies that complex nutrients can effectively maintain the body's telomerase activity, stabilize telomere length, and slow down the rate of cell aging, providing a solid animal experimental basis for its anti-aging effect.

[0047] Table 4 Results of GSH-PX activity determination of various examples and comparative examples in in vivo experiments

[0048] Table 5 Results of SOD activity determination of various embodiments and comparative examples in in vivo experiments

[0049] Table 6 Results of MDA activity determination of various embodiments and comparative examples in in vivo experiments

[0050] Table 7 NAD of each embodiment and comparative example in in vivo experiment + Assay results

[0051] Table 8 DNA damage determination results of various embodiments and comparative examples in in vivo experiments

[0052] Table 9 Telomerase activity measurement results of various examples and comparative examples in in vivo experiments

[0053] The preferred embodiments of the present invention are described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the technical concept of the present invention, a variety of simple modifications can be made to the technical solution of the present invention, and these simple modifications all belong to the protection scope of the present invention.

[0054] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, the present invention will not further describe various possible combinations.

[0055] In addition, various embodiments of the present invention may be arbitrarily combined, and as long as they do not violate the concept of the present invention, they should also be regarded as the contents disclosed by the present invention.

Claims

1. A composite nutrient with anti-aging function, characterized in that: The composition comprises the following components by weight: 15 to 25 parts of NMN powder, 10 to 20 parts of betaine, 5 to 15 parts of green tea extract, 5 to 15 parts of astragalus extract, 3 to 8 parts of burdock extract, 3 to 8 parts of sialic acid, 1 to 5 parts of ginseng root extract, and 1 to 3 parts of milk thistle extract.

2. The composite nutrient with anti-aging function according to claim 1, characterized in that: By mass, the composition includes the following components: 18 to 23 parts of NMN powder, 12 to 18 parts of betaine, 8 to 12 parts of green tea extract, 8 to 12 parts of astragalus extract, 4 to 6 parts of burdock extract, 4 to 6 parts of sialic acid, 2 to 4 parts of ginseng root extract, and 1 to 2 parts of milk thistle extract.

3. The composite nutrient with anti-aging function according to claim 2, characterized in that: The composition comprises the following components by mass: 20 parts of NMN powder, 15 parts of betaine, 10 parts of green tea extract, 10 parts of astragalus extract, 5 parts of burdock extract, 5 parts of sialic acid, 3 parts of ginseng root extract and 2 parts of milk thistle extract.

4. The composite nutrient with anti-aging function according to any one of claims 1 to 3, characterized in that: The green tea extract is EGCG.

5. The composite nutrient with anti-aging function according to any one of claims 1 to 3, characterized in that: The invention also includes the following components by weight: 5 to 10 parts of dextrin, 1 to 3 parts of microcrystalline cellulose, 0.5 to 2 parts of magnesium stearate, and 0.5 to 2 parts of silicon dioxide.

6. The composite nutrient with anti-aging function according to claim 5, characterized in that: The invention also includes the following components by weight: 6 parts of dextrin, 2 parts of microcrystalline cellulose, 1 part of magnesium stearate and 1 part of silicon dioxide.

7. The composite nutrient with anti-aging function according to any one of claims 1 to 3, characterized in that: The betaine extraction process is as follows: adding a solvent to dried and crushed beetroot and amaranth, soaking, continuously stirring, heating, filtering, distilling the filtrate, purifying, and drying to obtain betaine powder; The extraction process of the green tea extract is as follows: adding a solvent to dried and crushed green tea leaves, soaking, continuously stirring, heating, filtering, concentrating the filtrate, purifying, and drying to obtain green tea extract powder; The extraction process of the astragalus extract is as follows: adding a solvent to the dried and crushed astragalus, soaking, continuously stirring, heating, filtering, concentrating the filtrate, purifying, drying, and sieving to obtain the astragalus extract powder; The extraction process of the burdock extract is as follows: adding a solvent to dried and crushed fresh burdock roots, soaking, heating, filtering, concentrating the filtrate, purifying, drying, and sieving to obtain burdock extract powder; The extraction process of the ginseng root extract is as follows: adding a solvent to the dried and crushed ginseng root, soaking, reflux extraction, filtering, concentrating the filtrate, purifying, and drying to obtain ginseng root extract powder; The extraction process of the milk thistle extract is as follows: adding a solvent to dried and crushed milk thistle seeds, soaking, reflux extraction, filtering, concentrating the filtrate, and drying to obtain the milk thistle extract powder.

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