Yak cheese protein powder as well as preparation method and application thereof

Abstract

The invention relates to yak cheese protein powder as well as a preparation method and application thereof, and relates to the technical field of casein protein powder, the yak cheese protein powder comprises the following raw materials in parts by weight: 40-55 parts of yak cheese protein, 10-20 parts of lotus leaf extract and 3-7 parts of lutein. The yak milk casein, the lotus leaf extract and the xanthophyll are matched and used in a proper proportion and have a synergistic effect, and animal experiments show that the yak milk casein powder can effectively reduce the high beta-CTX level caused by OVX, relieve symptoms of an I-type osteoporosis animal model and improve the curative effect of the yak milk casein powder. Powerful support is provided for development of functional food, health care products or medicines and the like, and wide application prospects are achieved.

Description

Technical Field

[0001] The invention relates to the technical field of casein powder, and in particular to yak milk casein powder and a preparation method and application thereof. Background Art

[0002] In the field of casein powder technology, as people's health awareness continues to improve, the demand for foods, health products and medicines with specific health benefits is growing. Osteoporosis, as a common bone metabolic disease, seriously affects people's quality of life, especially type I osteoporosis, whose incidence rate is high. Therefore, the development of products with the effect of preventing and treating osteoporosis has important social and economic value.

[0003] As a mammal unique to the plateau, yaks' dairy products have attracted much attention due to their unique nutritional components and bioactive substances. Yak milk casein, as the main protein component in yak milk, not only has rich nutritional value, but may also contain bioactive peptides or other beneficial ingredients that are beneficial to bone health. However, there are relatively few deep-processed products of yak milk casein on the market, and the specific effects of yak milk casein on bone health have not been studied in depth.

[0004] On the other hand, lotus leaf extract and lutein, as natural plant ingredients, are also widely used in food, health products and medicine. Lotus leaf extract has the effects of clearing away heat and dampness, and anti-oxidation, while lutein is an important antioxidant that plays a positive role in protecting the eyes and preventing cardiovascular diseases.

[0005] However, there is no report in the prior art on the combined use of yak milk casein, lotus leaf extract and lutein for the prevention and treatment of osteoporosis. Summary of the invention

[0006] In order to solve the above problems, the present invention provides a yak cheese protein powder and a preparation method and application thereof.

[0007] In a first aspect, the present invention provides a yak cheese protein powder, which comprises the following raw materials in parts by weight:

[0008] 40-55 parts of yak milk casein, 10-20 parts of lotus leaf extract and 3-7 parts of lutein.

[0009] Furthermore, the yak cheese protein powder comprises the following raw materials in parts by weight:

[0010] 48 parts of yak milk casein, 16 parts of lotus leaf extract and 5 parts of lutein.

[0011] Furthermore, the lotus leaf extract is a lotus leaf water extract.

[0012] Furthermore, the lotus leaf water extract is obtained by using lotus leaf as raw material and water as extraction solvent through reduced pressure reflux extraction.

[0013] Furthermore, the working condition parameters of the reduced pressure reflux extraction include: a pressure of 10 to 20 kPa, a temperature of 50 to 65° C., 1 to 3 extraction times, and a weight ratio of the extraction solvent to the lotus leaf of (5 to 10):1.

[0014] Furthermore, the working condition parameters of the reduced pressure reflux extraction include: pressure of 15 kPa, temperature of 60° C., time of 60 to 90 minutes, number of extractions of 1 to 3 times, and a weight ratio of the extraction solvent to the lotus leaf of (5 to 10):1.

[0015] In a second aspect, the present invention provides a method for preparing the yak cheese protein powder according to any one of the first aspects, the preparation method comprising the following steps:

[0016] obtaining a lotus leaf extract;

[0017] Adding the lotus leaf extract, yak milk casein and lutein into water and stirring and mixing to obtain a first mixture;

[0018] Adding auxiliary materials to the first mixed material and stirring and mixing them to obtain a second mixed material;

[0019] The second mixed material is homogenized and then spray-dried to obtain the yak cheese protein powder.

[0020] Furthermore, in terms of weight percentage, the concentration of yak casein in the first mixture is 4-7%.

[0021] Furthermore, the auxiliary material includes β-cyclodextrin, and the added weight of the β-cyclodextrin accounts for 0.8 to 1.1 times the total weight of the lotus leaf extract, the yak casein and the lutein.

[0022] In a third aspect, the present invention provides an application of the yak casein powder described in any one of the first aspect or the yak casein powder prepared by the preparation method of the yak casein powder described in any one of the second aspect in the preparation of a product for improving the symptoms of type I osteoporosis, wherein the product comprises at least one of a medicine, a functional food and a health product.

[0023] The above technical solution provided by the embodiment of the present invention has at least the following advantages compared with the prior art:

[0024] The embodiment of the present invention provides a yak casein protein powder and a preparation method and application thereof. The present invention uses yak casein, lotus leaf extract and lutein in appropriate proportions, and the three have a synergistic effect. Animal experiments show that the yak casein protein powder can effectively reduce the high β-CTX level caused by OVX and alleviate the symptoms of type I osteoporosis animal models, providing strong support for its development into functional foods, health products or medicines, etc., and has broad application prospects. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate embodiments consistent with the invention and, together with the description, serve to explain the principles of the invention.

[0026] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, for ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative labor.

[0027] Figure 1 A schematic flow chart of a method for preparing yak cheese protein powder provided in an embodiment of the present invention. DETAILED DESCRIPTION

[0028] In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the technical solution in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0029] Unless otherwise specified, various raw materials, reagents, instruments and equipment used in the present invention can be purchased from the market or prepared by existing methods.

[0030] In a first aspect, the present invention provides a yak cheese protein powder, which comprises the following raw materials in parts by weight:

[0031] 40-55 parts of yak milk casein, 10-20 parts of lotus leaf extract and 3-7 parts of lutein.

[0032] The embodiment of the present invention provides a yak casein protein powder. The present invention uses yak casein, lotus leaf extract and lutein in appropriate proportions, and the three have a synergistic effect. Animal experiments show that the yak casein protein powder can effectively reduce the high β-CTX level caused by OVX and alleviate the symptoms of type I osteoporosis animal models, providing strong support for its development into functional foods, health products or medicines, etc., and has broad application prospects.

[0033] The yak milk casein in the present invention can be directly a food-grade commercial product or can be prepared in-house according to existing technologies such as CN102726592A-A method for preparing yak milk casein. Specifically, the yak milk casein in the subsequent examples and comparative examples of the present invention is prepared according to the method described in Example 1 of CN102726592A.

[0034] In some specific embodiments, the yak cheese protein powder includes the following raw materials in parts by weight:

[0035] 48 parts of yak milk casein, 16 parts of lotus leaf extract and 5 parts of lutein.

[0036] In some specific embodiments, the lotus leaf extract is lotus leaf water extract.

[0037] In some specific embodiments, the lotus leaf water extract is obtained by using lotus leaf as raw material and water as extraction solvent through reduced pressure reflux extraction.

[0038] In some specific embodiments, the working condition parameters of the reduced pressure reflux extraction include: pressure of 10-20 kPa, temperature of 50-65°C, number of extractions of 1-3 times, and a weight ratio of the extraction solvent to the lotus leaf of (5-10):1.

[0039] In some specific embodiments, the working condition parameters of the reduced pressure reflux extraction include: pressure of 15 kPa, temperature of 60°C, time of 60 to 90 minutes, number of extractions of 1 to 3 times, and a weight ratio of the extraction solvent to the lotus leaf of (5 to 10):1.

[0040] In a second aspect, based on the same inventive concept, the present invention provides a method for preparing the yak cheese protein powder according to any one of the first aspects, such as Figure 1 As shown, the preparation method comprises the following steps:

[0041] obtaining a lotus leaf extract;

[0042] Adding the lotus leaf extract, yak milk casein and lutein into water and stirring and mixing to obtain a first mixture;

[0043] Adding auxiliary materials to the first mixed material and stirring and mixing them to obtain a second mixed material;

[0044] The second mixed material is homogenized and then spray-dried to obtain the yak cheese protein powder.

[0045] The method for preparing yak cheese protein powder provided by the embodiment of the present invention is simple to operate, does not require additional specific equipment, and is suitable for industrial production.

[0046] In some specific embodiments, the concentration of yak casein in the first mixture is 4-7% by weight.

[0047] In some specific embodiments, the auxiliary material includes β-cyclodextrin, and the added weight of the β-cyclodextrin is 0.8 to 1.1 times the total weight of the lotus leaf extract, the yak casein and the lutein.

[0048] In some specific embodiments, the working condition parameters of the homogenization treatment include: a pressure of 12 MPa; the working condition parameters of the spray drying include: an inlet air temperature of 172°C and an exhaust air temperature of 88°C.

[0049] In the third aspect, based on the same inventive concept, the present invention provides an application of the yak casein powder described in any one of the first aspect or the yak casein powder prepared by the preparation method of the yak casein powder described in any one of the second aspect in the preparation of a product for improving the symptoms of type I osteoporosis, wherein the product includes at least one of a medicine, a functional food and a health product.

[0050] It should be noted that the raw material components involved in the yak cheese protein powder and its preparation method and application provided by the embodiments of the present invention, unless otherwise specified or described, can be directly purchased from the market or made at home according to existing preparation methods; at the same time, the operating steps involved in the preparation method, unless otherwise specified or described, can be carried out according to existing processes or using commercially available equipment, and the present invention document will not describe them one by one.

[0051] The present invention will be further described below in conjunction with specific examples. It should be understood that these examples are intended only to illustrate the present invention and are not intended to limit the scope of the present invention. The experimental methods in the following examples that do not specify specific conditions are usually measured according to national standards. If there is no corresponding national standard, then the conditions recommended by the manufacturer are followed.

[0052] Example 1

[0053] This example provides a yak cheese protein powder, which includes the following raw materials in parts by weight:

[0054] 48 parts of yak milk casein, 16 parts of lotus leaf extract and 5 parts of lutein;

[0055] The preparation method of the lotus leaf extract comprises the following steps: crushing the dried lotus leaves and adding them into water, stirring and mixing, wherein the weight ratio of the water to the lotus leaves is 8:1, and then performing reduced pressure reflux extraction for 75 minutes at a pressure of 15 kPa and a temperature of 60° C., and the extraction times are 2 times; combining the obtained extracts, concentrating and drying to a constant weight, crushing and passing through a 100-mesh sieve, and obtaining the lotus leaf extract.

[0056] The preparation method of the above-mentioned yak cheese protein powder comprises the following steps:

[0057] obtaining a lotus leaf extract;

[0058] Adding the lotus leaf extract, yak casein and lutein into water and stirring and mixing, to obtain a first mixed material containing 5% by weight of yak casein;

[0059] Adding β-cyclodextrin to the first mixed material and stirring and mixing, wherein the added weight of the β-cyclodextrin accounts for 1 times the total weight of the lotus leaf extract, the yak casein and the lutein, to obtain a second mixed material;

[0060] The second mixed material is homogenized and then spray-dried to obtain the yak cheese protein powder.

[0061] Example 2

[0062] This example provides a yak cheese protein powder, which includes the following raw materials in parts by weight:

[0063] 40 parts of yak milk casein, 10 parts of lotus leaf extract and 3 parts of lutein;

[0064] The preparation method of the lotus leaf extract comprises the following steps: crushing the dried lotus leaves and adding them into water, stirring and mixing, wherein the weight ratio of the water to the lotus leaves is 5:1, and then performing reduced pressure reflux extraction for 90 minutes at a pressure of 10 kPa and a temperature of 50° C., and the extraction times are 2 times; combining the obtained extracts, concentrating and drying to constant weight, crushing and passing through a 100-mesh sieve, and obtaining the lotus leaf extract.

[0065] The preparation method of the above-mentioned yak cheese protein powder comprises the following steps:

[0066] obtaining a lotus leaf extract;

[0067] Adding the lotus leaf extract, yak casein and lutein into water and stirring and mixing, to obtain a first mixed material containing 4% by weight of yak casein;

[0068] Adding β-cyclodextrin to the first mixed material and stirring and mixing, wherein the added weight of the β-cyclodextrin accounts for 0.8 times the total weight of the lotus leaf extract, the yak casein and the lutein, to obtain a second mixed material;

[0069] The second mixed material is homogenized and then spray-dried to obtain the yak cheese protein powder.

[0070] Example 3

[0071] This example provides a yak cheese protein powder, which includes the following raw materials in parts by weight:

[0072] 55 parts of yak milk casein, 20 parts of lotus leaf extract and 7 parts of lutein;

[0073] The preparation method of the lotus leaf extract comprises the following steps: crushing the dried lotus leaves and adding them into water, stirring and mixing, wherein the weight ratio of the water to the lotus leaves is 10:1, and then performing reduced pressure reflux extraction for 60 minutes at a pressure of 20 kPa and a temperature of 65° C., and the extraction times are 2 times; combining the obtained extracts, concentrating and drying to constant weight, crushing and passing through a 100-mesh sieve, and obtaining the lotus leaf extract.

[0074] The preparation method of the above-mentioned yak cheese protein powder comprises the following steps:

[0075] obtaining a lotus leaf extract;

[0076] Adding the lotus leaf extract, yak casein and lutein into water and stirring and mixing, to obtain a first mixed material containing 7% by weight of yak casein;

[0077] Adding β-cyclodextrin to the first mixed material and stirring and mixing, wherein the added weight of the β-cyclodextrin accounts for 1.1 times the total weight of the lotus leaf extract, the yak casein and the lutein, to obtain a second mixed material;

[0078] The second mixed material is homogenized and then spray-dried to obtain the yak cheese protein powder.

[0079] Comparative Example 1

[0080] This example provides a yak cheese protein powder and a preparation method thereof, which is different from Example 1 only in that the weight proportion of lotus leaf extract is adjusted to 5 parts, and the weight proportion of lutein is adjusted to 16 parts; the remaining steps and parameters are the same.

[0081] Comparative Example 2

[0082] This example provides a yak casein protein powder and a preparation method thereof, which is different from Example 1 only in that the pressure in the preparation method of the lotus leaf extract is adjusted to 51 kPa and the temperature is adjusted to 85° C.; the remaining steps and parameters are the same.

[0083] Test Case

[0084] This example investigates the intervention effect of the yak casein protein powder obtained in the above-mentioned embodiments and comparative examples on the osteoporosis symptoms of type I osteoporosis animal models according to the test method disclosed in the prior art.

[0085] Test method:

[0086] 1. Experimental groups: blank control group: C57 / B6J normal healthy mice, female, 20 weeks old, 10 mice; model control group: 20-week-old bilaterally ovariectomized mice, used for type I (postmenopausal) osteoporosis model control, 10 mice; drug intervention group: 20-week-old bilaterally ovariectomized mice, given oral administration of yak casein powder samples obtained in the examples and comparative examples (the sample given in Example 1 was recorded as Example 1 group, the sample given in Example 2 was recorded as Example 2 group, the sample given in Example 3 was recorded as Example 3 group, the sample given in Comparative Example 1 was recorded as Comparative Example 1 group, and the sample given in Comparative Example 2 was recorded as Comparative Example 2 group), the dose was 10 mg / kg, once a day, for a total of 2 months, and each sample group had 10 mice.

[0087] 2. Detection and data recording and analysis: The ELISA method was used to detect the serum β-CTX level of mice in each experimental group for statistical analysis. The test results are shown in Table 1.

[0088] Table 1

[0089]

[0090]

[0091] As shown in Table 1, compared with Comparative Examples 1 to 2, the yak casein powder samples provided in Examples 1 to 3 of the present invention have a more significant effect on reducing the high β-CTX level caused by OVX. In addition, the bone density, bone volume fraction and number of trabeculae of the femur of mice given the yak casein powder samples provided in Examples 1 to 3 were significantly restored, indicating that the yak casein powder provided in the embodiments of the present invention can significantly improve the symptoms of the type I osteoporosis animal model.

[0092] In summary, the embodiments of the present invention provide a yak casein protein powder and a preparation method and application thereof. The present invention uses yak casein, lotus leaf extract and lutein in appropriate proportions, and the three have a synergistic effect. Animal experiments have shown that the yak casein protein powder can effectively reduce the high β-CTX level caused by OVX and alleviate the symptoms of type I osteoporosis animal models, which provides strong support for its development into functional foods, health products or medicines, etc., and has broad application prospects.

[0093] Various embodiments of the present invention may be presented in the form of a range; it should be understood that the description in the form of a range is only for convenience and brevity, and should not be understood as a rigid limitation on the scope of the present invention; therefore, the range description should be considered to have specifically disclosed all possible sub-ranges and single numerical values ​​within the range. For example, the range description from 1 to 6 should be considered to have specifically disclosed sub-ranges, such as from 1 to 3, from 1 to 4, from 1 to 5, from 2 to 4, from 2 to 6, from 3 to 6, etc., as well as single numbers within the range, such as 1, 2, 3, 4, 5 and 6, which apply regardless of the range. In addition, whenever a numerical range is indicated herein, it is meant to include any cited number (fractional or integer) within the indicated range.

[0094] The foregoing is merely a specific embodiment of the present invention, which enables those skilled in the art to understand or implement the present invention. Various modifications to these embodiments will be apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but rather to the widest scope consistent with the principles and novel features claimed herein.

Claims

1. A yak cheese protein powder, characterized in that: The yak cheese protein powder comprises the following raw materials in parts by weight: 40-55 parts of yak milk casein, 10-20 parts of lotus leaf extract and 3-7 parts of lutein.

2. The yak cheese protein powder according to claim 1, characterized in that The yak cheese protein powder comprises the following raw materials in parts by weight: 48 parts of yak milk casein, 16 parts of lotus leaf extract and 5 parts of lutein.

3. The yak cheese protein powder according to claim 1 or 2, characterized in that The lotus leaf extract is a lotus leaf water extract.

4. The yak cheese protein powder according to claim 3, characterized in that The lotus leaf water extract is obtained by taking lotus leaf as raw material and water as extraction solvent through reduced pressure reflux extraction.

5. The yak cheese protein powder according to claim 4, characterized in that The working condition parameters of the reduced pressure reflux extraction include: pressure of 10-20 kPa, temperature of 50-65° C., extraction times of 1-3 times, and a weight ratio of the extraction solvent to the lotus leaf of (5-10):

1.

6. The yak cheese protein powder according to claim 4, characterized in that The working condition parameters of the reduced pressure reflux extraction include: pressure of 15 kPa, temperature of 60° C., time of 60 to 90 minutes, number of extractions of 1 to 3 times, and a weight ratio of the extraction solvent to the lotus leaf of (5 to 10):

1.

7. A method for preparing yak cheese protein powder according to any one of claims 1 to 6, characterized in that: The preparation method comprises the following steps: obtaining a lotus leaf extract; Adding the lotus leaf extract, yak milk casein and lutein into water and stirring and mixing to obtain a first mixture; Adding auxiliary materials to the first mixed material and stirring and mixing them to obtain a second mixed material; The second mixed material is homogenized and then spray-dried to obtain the yak cheese protein powder.

8. The method for preparing yak cheese protein powder according to claim 7, characterized in that: Calculated in weight percentage, the concentration of yak casein in the first mixture is 4-7%.

9. The method for preparing yak cheese protein powder according to claim 7, characterized in that: The auxiliary material comprises beta-cyclodextrin, and the added weight of the beta-cyclodextrin accounts for 0.8 to 1.1 times the total weight of the lotus leaf extract, the yak milk casein and the lutein.

10. Use of the yak casein powder according to any one of claims 1 to 6 or the yak casein powder prepared by the method for preparing the yak casein powder according to any one of claims 7 to 9 in preparing a product for improving the symptoms of type I osteoporosis, characterized in that: The product includes at least one of a medicine, a functional food and a health product.

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