Traditional Chinese medicine composition for treating mycoplasma diseases of sheep as well as preparation method and application of traditional Chinese medicine composition

Abstract

The invention discloses a traditional Chinese medicine composition for treating sheep mycoplasma diseases as well as a preparation method and application thereof, and belongs to the technical field of traditional Chinese medicines. According to the traditional Chinese medicine composition, sheep mycoplasmal pneumonia infection is analyzed and dialectically analyzed in cooperation with the thought of traditional Chinese veterinary medicine in clinical treatment, a traditional Chinese medicine prescription is selected, and honeysuckle, ephedra, scutellaria baicalensis, astragalus membranaceus, fritillaria, pinellia ternate and liquorice are compounded according to the traditional Chinese veterinary medicine dialectical theory and a monarch, minister, assistant and guide compatibility method, so that the traditional Chinese medicine composition is prepared. The invention provides a traditional Chinese medicine composition for treating mycoplasma pneumonia infection of sheep, and the traditional Chinese medicine composition can effectively treat mycoplasma pneumonia infection of sheep.

Description

Technical Field

[0001] The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a traditional Chinese medicine composition for treating sheep mycoplasma disease, and a preparation method and application thereof. Background Art

[0002] Mycoplasma pneumonia in sheep, also known as contagious pleuropneumonia in sheep, is caused by Mycoplasma filamentosa, which causes sheep to have a respiratory infectious disease characterized by coughing, high fever, and pleurisy. It is highly contagious and spreads rapidly in the sheep flock through direct contact and respiratory droplets, causing serious damage to the sheep farming industry. In previous treatments, various antibacterial drugs were often used for administration, but Western medicine treatments are prone to drug resistance in sheep and have the disadvantage of large toxic side effects. In the long run, it is not conducive to the prevention and treatment of the disease.

[0003] Traditional Chinese veterinary medicine originated in ancient China and has been formed after thousands of years of development. The "monarch, minister, assistant and envoy" of traditional Chinese medicine is a method of combining traditional Chinese medicine prescriptions. The main ingredient in the medicine is the monarch, the auxiliary ingredient is the minister, the auxiliary ingredient is the assistant ingredient, and the envoy ingredient is the one that helps the main medicine treat incidental diseases and inhibits the adverse effects of the main medicine. Therefore, in clinical treatment, the disease can be analyzed and dialectically analyzed with the thinking of traditional Chinese veterinary medicine, combined with the method of combining traditional Chinese medicine prescriptions, to screen low-toxic, efficient and safe anti-mycoplasma traditional Chinese medicine or traditional Chinese medicine combination, to prevent or treat sheep mycoplasma pneumonia, thereby reducing the occurrence of pathogen resistance, improving the health of sick sheep, and reducing the losses of farmers. Summary of the invention

[0004] In view of the above-mentioned prior art, the purpose of the present invention is to provide a traditional Chinese medicine composition for treating sheep mycoplasma disease and its preparation method and application.

[0005] To achieve the above object, the present invention adopts the following technical solutions:

[0006] In a first aspect, the present invention provides a traditional Chinese medicine composition for treating mycoplasma diseases in livestock, wherein the traditional Chinese medicine composition is composed of the following raw materials in parts by weight: 30-50 parts of honeysuckle, 30-50 parts of ephedra, 20-35 parts of scutellaria, 20-35 parts of astragalus, 15-30 parts of fritillaria, 15-30 parts of pinellia, and 10-30 parts of liquorice.

[0007] The honeysuckle and ephedra are compounded as the main medicine, the scutellaria and astragalus are compounded as the assistant medicine, the fritillaria and pinellia are compounded as the adjuvant medicine, and the liquorice is the guiding medicine.

[0008] Preferably, the Chinese medicine composition is composed of the following raw materials in parts by weight: 30 parts of honeysuckle, 30 parts of ephedra, 20 parts of scutellaria, 20 parts of astragalus, 15 parts of fritillaria, 15 parts of pinellia, and 20 parts of liquorice.

[0009] The livestock is sheep.

[0010] The mycoplasma disease is ovine mycoplasma pneumonia.

[0011] The second aspect of the present invention provides a method for preparing the above-mentioned Chinese medicine composition, comprising the following steps:

[0012] Honeysuckle, ephedra, scutellaria, astragalus, fritillaria, pinellia and liquorice are dried, crushed and sieved respectively, compounded according to weight proportions, and boiled and concentrated with water until the concentration of the medicinal solution reaches 1g / mL.

[0013] The third aspect of the present invention provides the use of the above-mentioned traditional Chinese medicine composition in the preparation of a drug for treating mycoplasma diseases in livestock.

[0014] In a fourth aspect, the present invention provides a drug for treating mycoplasma diseases in livestock, wherein the drug comprises the above-mentioned traditional Chinese medicine composition as an active ingredient.

[0015] The medicament may further include pharmaceutically acceptable excipients.

[0016] The pharmaceutically acceptable excipients are disintegrants, lubricants, sweeteners or binders.

[0017] The dosage form of the medicine is solution, soluble powder or injection.

[0018] Beneficial effects of the present invention:

[0019] 1. The present invention provides a Chinese medicine composition for treating sheep mycoplasma pneumonia infection according to the syndrome differentiation theory of traditional Chinese veterinary medicine and the compatibility method of "monarch, minister, assistant and messenger". The composition comprises honeysuckle, which is sweet and cold, enters the lung and stomach meridian, dispels wind-heat, and is good at clearing blood poison; ephedra is bitter and warm, enters the lung and bladder meridian, sweats and dispels cold, dispels lung heat and relieves asthma, promotes diuresis and reduces swelling, and ephedra helps honeysuckle to clear lung heat, and the two are monarch medicines. Scutellaria baicalensis is bitter and cold, clears heat and dries dampness, purges fire and detoxifies, and treats high fever and thirst, lung heat cough, damp-heat diarrhea, and hot stranguria; Astragalus: sweet and warm, internally tonifies the spleen and lung qi, externally can consolidate the exterior and stop sweating, and helps the monarch medicine to clear the lung and clear heat with Scutellaria baicalensis. Fritillaria thunbergii is bitter and cold, enters the heart and lung meridian, clears heat and resolves phlegm, and Pinellia ternata is pungent and warm, enters the spleen and stomach meridian, dries dampness and resolves phlegm, and the two are adjuvants, clearing the lung and resolving phlegm. Licorice is sweet and mild, belongs to the twelve meridians, clears away heat and detoxifies, moistens the lungs and relieves cough, nourishes the spleen and replenishes qi, and harmonizes the properties of various medicines. The seven herbs are used together to play the role of relieving the exterior, promoting lung function, detoxifying and relieving asthma, and can effectively relieve the fever, cough, asthma, fatigue and other symptoms caused by sheep mycoplasma pneumonia.

[0020] 2. In clinical treatment, the present invention cooperates with the idea of ​​traditional Chinese veterinary medicine to analyze and dialectically treat sheep mycoplasma pneumonia infection, selects traditional Chinese medicine prescriptions for treatment and prevention, and uses the combination of "monarch, minister, assistant and messenger" four medicines to exert a synergistic effect and effectively treat sheep mycoplasma disease infection. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 : Distribution of drug properties in effective prescriptions for the treatment of mycoplasma infection in sheep.

[0022] Figure 2 : Distribution of medicinal flavors of drugs in effective prescriptions for the treatment of mycoplasma infection in sheep.

[0023] Figure 3 : The meridian distribution of drugs in effective prescriptions for the treatment of sheep mycoplasma infection.

[0024] Figure 4 : Cluster diagram of traditional Chinese medicine variables.

[0025] Figure 5 : Venny diagram of active ingredient targets of traditional Chinese medicine composition and mycoplasma pneumonia infection targets.

[0026] Figure 6 : PPI network of potential targets of Chinese herbal medicine composition for the treatment of sheep Mycoplasma infection; among them, the redder the color, the higher the ranking of the target and the more important the target.

[0027] Figure 7 : Key targets of Chinese herbal medicine composition in treating sheep Mycoplasma infection.

[0028] Figure 8 : GO functional analysis; the green part represents the biological process, the orange part represents the cellular component, and the purple part represents the molecular function.

[0029] Fig. 9 : KEGG enrichment analysis; the larger the bubble area, the more enriched genes there are; the redder the bubble color, the more significant the enrichment.

[0030] Fig.10 : Molecular docking binding energy diagram.

[0031] Fig.11 : Molecular docking diagram; the red part in the diagram is the small molecule ligand, and the purple part is the macromolecular receptor residue; the redder the color, the greater the binding energy and the stronger the docking activity. DETAILED DESCRIPTION

[0032] It should be noted that the following detailed descriptions are illustrative and are intended to provide further explanation of the present application. Unless otherwise specified, all technical and scientific terms used herein have the same meanings as those commonly understood by those skilled in the art to which the present application belongs.

[0033] As mentioned above, sheep mycoplasma pneumonia is a common infectious disease in sheep breeding. Once the sheep are infected with the disease, it will not only affect the health of the sheep, but also cause death, causing serious losses to the farmers. Western medicine treatment can easily make sheep resistant and has the disadvantage of large toxic and side effects. In the long run, it is not conducive to the prevention and treatment of the disease. Therefore, it is of great significance to analyze and dialectically analyze the disease with the thinking of traditional Chinese veterinary medicine, combine the compatibility method of traditional Chinese medicine prescriptions, screen low-toxic, efficient and safe anti-mycoplasma Chinese medicine or Chinese medicine composition, and prevent or treat sheep mycoplasma pneumonia.

[0034] Based on this, the present invention provides a Chinese medicine composition for treating sheep mycoplasma disease, and its preparation method and application. The present invention first conducts preliminary data research, sorts out the academic journals, dissertations and other documents currently published, and screens the relevant prescriptions to obtain 141 Chinese medicine prescriptions, including 169 Chinese medicines. The frequency of use of these 169 medicines was counted, and it was found that the top 10 medicines in terms of frequency of use were licorice (89 times), scutellaria (75 times), apricot (63 times), platycodon (61 times), honeysuckle (59 times), forsythia (46 times), ephedra (36 times), houttuynia cordata (34 times), Fritillaria thunbergii (31 times), and astragalus (31 times).

[0035] Table 1 Distribution of effective prescriptions for the treatment of sheep mycoplasma infection with a frequency greater than 10 for traditional Chinese medicine

[0036]

[0037]

[0038] The medicinal properties of traditional Chinese medicine include cold, warm, cool, and hot. In addition, there are medicines with neutral properties. Each medicine corresponds to only one medicinal property. Through statistical analysis of the distribution of medicinal properties of 169 traditional Chinese medicines, it was found that cold, warm, and neutral are the three most frequently used medicinal properties ( Figure 1 ). Among them, the frequency of use of cold drugs was 49% (82 times), the frequency of use of warm drugs was 28% (47 times), the frequency of use of neutral drugs was 16% (27 times), the frequency of use of cool drugs was 6% (11 times), and the frequency of use of hot drugs was 1% (2 times).

[0039] The taste of Chinese medicine includes five types: pungent, sweet, bitter, sour, and salty. Each medicine can have multiple tastes. Through statistical analysis of the distribution of the taste of 169 Chinese medicines, it was found that bitter, sweet, and pungent are the three most frequently occurring tastes ( Figure 2 Among them, bitter drugs accounted for 40% (95 times), sweet drugs accounted for 30% (70 times), pungent drugs accounted for 25% (60 times), sour drugs accounted for 3% (8 times), and salty drugs accounted for 2% (5 times).

[0040] Screening of Chinese herbal compound prescriptions based on data mining and network pharmacology and its therapeutic effect on sheep mycoplasma infection Each Chinese herbal medicine has one or more meridians, and the distribution of meridians of 169 Chinese herbal medicines was statistically analyzed ( Figure 3 ). It was found that the drugs belonging to the lung meridian were used most frequently, with a frequency of 87 times (accounting for 22%); followed by drugs belonging to the liver meridian, with a frequency of 69 (accounting for 17%); the frequency of drugs belonging to the stomach meridian was 61 (accounting for 15%), the frequency of drugs belonging to the spleen meridian was 54 (accounting for 14%), the frequency of drugs belonging to the heart meridian was 37 (accounting for 9%), the frequency of drugs belonging to the kidney meridian was 32 (accounting for 8%), the frequency of drugs belonging to the large intestine meridian was 25 (accounting for 6%), the frequency of drugs belonging to the bladder meridian was 17 (accounting for 4%), the frequency of drugs belonging to the gallbladder meridian was 12 (accounting for 3%), the frequency of drugs belonging to the small intestine meridian was 3 (accounting for 1%), and the frequency of drugs belonging to the triple burner was 2 (accounting for 1%).

[0041] SPSS Modeler was used to analyze the association rules of 141 prescriptions, and the top 20 drugs were analyzed according to the association rules to obtain the medication patterns and associated medication combinations. Among them, the number of supports refers to the frequency of drug combinations in prescriptions, and the confidence is the correlation between drugs. The minimum conditional support is set to 10 and the confidence is set to 0.8. The results are as follows. Medication combination pattern: According to the above parameter settings, 34 commonly used Chinese medicine combination patterns were obtained. Among them, there are 9 commonly used combination patterns of two Chinese medicines; 20 combination patterns of three Chinese medicines; and 13 combination patterns of four Chinese medicines. The results are shown in Tables 2, 3, and 4. Among them, 17 groups of drugs contain honeysuckle.

[0042] Table 2: Combination patterns of two Chinese medicines in prescriptions

[0043]

[0044] Table 3: Combination pattern of three Chinese medicines in prescription

[0045]

[0046] Table 4: Combination pattern of four traditional Chinese medicines in prescription

[0047]

[0048]

[0049] We continued to conduct a systematic cluster analysis on the top 20 drugs in terms of frequency of use. The results are as follows: Figure 4 The top 20 drugs with the highest frequency of use are divided into five categories. The first category includes honeysuckle, forsythia, loquat leaf, platycodon, and schizonepeta; the second category includes apricot kernel, ephedra, and gypsum; the third category includes peucedanum, pinellia, and astragalus; the fourth category includes houttuynia cordata, mulberry bark, scutellaria, anemarrhena, and ophiopogon; the fifth category includes licorice, gardenia, isatis root, and fritillaria.

[0050] The present invention statistically analyzes the medicinal properties, taste, meridians and efficacy of each medicine according to the 2020 edition of the Veterinary Pharmacopoeia of the People's Republic of China, performs association rule analysis and cluster analysis according to the frequency of the medicine, analyzes each result and combines the compatibility principle of "monarch, minister, assistant and messenger" to form a self-made new prescription: monarch medicine-honeysuckle, ephedra; minister medicine-scutellaria, astragalus; adjuvant medicine-fritillaria, pinellia; messenger medicine-licorice. On the basis of the self-made new prescription, it is combined and compounded according to the formula amount to obtain a Chinese medicine composition for treating sheep mycoplasma disease.

[0051] The following detailed description is for illustrative purposes only and is intended to provide further explanation of the present application, rather than to limit the scope of the present invention.

[0052] Example 1: Preparation of Chinese medicine composition

[0053] Dry and crush honeysuckle, ephedra, scutellaria, astragalus, fritillaria, pinellia and licorice respectively, and pass through a 50-mesh sieve; then compound 30 grams of honeysuckle and 30 grams of ephedra as the main medicine, 20 grams of scutellaria and 20 grams of astragalus as the ministerial medicine, 15 grams of fritillaria and 15 grams of pinellia as the adjuvant medicine, and 20 grams of licorice as the guiding medicine; add water, decoct and concentrate, filter, discard the filter residue, and take the supernatant with a drug concentration of 1 g / mL to obtain the product.

[0054] Example 2: Analysis of the active ingredients of Chinese medicine compositions and disease-related targets and pathways

[0055] The Chinese medicine composition prepared in Example 1 was screened with oral bioavailability (OB) ≥ 30%, drug-likeness (DL) ≥ 0.18 in the TCMSP database and ingredients collected in the ETCM database with drug-likeness graded as "Good", and a total of 136 active ingredients were obtained (Table 5).

[0056] Table 5: Active ingredients of Chinese medicine composition

[0057]

[0058]

[0059]

[0060] We continued to predict the targets of the Chinese medicine combination in the SwissTargetPrediction database, and after removing duplicate values, we obtained 872 targets.

[0061] The human gene database (GeneCard, https: / / www.genecards.org / ), the Online Mendelian Inheritance in Man (OMIM, http: / / omim.org / ) and the Comparative Toxicogenomics Database (CTD, https: / / ctdbase.org / ) were searched and summarized using the keyword "Mycoplasma infection of sheep", and 704 sheep Mycoplasma infection-related disease targets were collected.

[0062] The VENNY platform was used to intersect and visualize the targets of the active ingredients of the traditional Chinese medicine composition and the targets of sheep Mycoplasma infection. A total of 61 potential targets related to sheep Mycoplasma infection were obtained for the important composition prepared by the present invention. The results are as follows: Figure 5 .

[0063] For the 136 active ingredients corresponding to the 61 potential targets obtained, a "drug-active ingredient-potential target" network was constructed. There were 204 nodes and 911 edges in the network. The MCC algorithm (Maximum Clique Centrality) was used to identify the central nodes in the cluster. The top-ranked compounds included quercetin, pectolinarigenin, oroxylin A, neobaicalein, licochalcone A, etc. It was speculated that these ingredients were the main active ingredients of the traditional Chinese medicine composition for the treatment of sheep mycoplasma infection.

[0064] 61 potential targets were imported into the STRING (https: / / string-db.org / ) database to construct a protein-protein interaction network. The species "Gallus gallus" was selected with a minimum interaction score (combined score) > 0.4 as the standard for screening. Other parameters were selected as default and free targets were eliminated. Then the TSV format file was downloaded and imported into Cystscope3.9.1 for visualization. The results are shown in the figure. Figure 6 The network involves 45 nodes and 131 edges. The MCC of each node is calculated by the plug-in cytohubba in Cytoscape. 20 core targets are screened out. According to the comprehensive ranking, the top 7 targets are CASP3, STAT3, STAT1, TGFB1, HIF1A, GAPDH, and PTGS2, which are the core targets for the treatment of sheep mycoplasma infection ( Figure 7 ).

[0065] 61 potential targets were imported into the DAVID database and screened with P < 0.01 and FDR < 0.05, and a total of 119 GO enrichment items were obtained, including 78 biological processes (BP), mainly including the positive regulation of transcription by RNA polymerase II, apoptosis process, negative regulation of transcription by RNA polymerase II, positive regulation of gene expression, negative regulation of apoptosis process, positive regulation of DNA template transcription, signal transduction, inflammatory response, negative regulation of cell population proliferation, phosphorylation, etc.; 12 cell components (CC), mainly involving cytoplasm, cell surface cytoplasm, protein-containing complex, extracellular region, transcriptional regulatory complex, RNA polymerase II transcriptional regulatory complex, extracellular space, nucleus, outer side of plasma membrane, nucleoplasm, chromatin; 19 molecular functions (Molecular Function, MF), mainly involving enzyme binding, same protein binding, protein binding, protein homodimerization activity, heme binding, nuclear receptor activity, protease binding, serine endopeptidase activity, DNA binding transcription factor activity, protein phosphatase binding and other aspects. The top GO items were selected to draw a bar graph. The results showed that the traditional Chinese medicine composition provided by the present invention may play a therapeutic role in sheep mycoplasma infection through positive regulation of gene expression, signal transduction, inflammatory response, negative regulation of cell population proliferation and protein phosphorylation ( Figure 8 ).

[0066] The signal pathways screened in the KEGG pathway enrichment analysis (P < 0.01) were used to draw a bubble chart of the top 10 entries. The pathways related to mycoplasma ovis infection mainly included MAPK signaling pathway, apoptosis, T cell receptor signaling pathway, phosphatidylinositol 3-kinase-protein kinase pathway, proteoglycans in cancer, etc. ( Fig. 9 ).

[0067] In order to verify the accuracy of the network pharmacology prediction results, the predicted core targets CASP3, STAT3, STAT1 and the main active ingredients in NCMC, Quercetin, Pectolinarigenin, Oroxylin A, Neobaicalein, and Licochalcone A, were selected for molecular docking. It is generally believed that the redder the color, the lower the docking score and the stronger the docking activity. The results are shown in Figure 2. Fig.10 The molecular docking results showed that hydrogen bonds were formed between the main active ingredients and the core targets, indicating that they had good binding activity. Fig.11, Table 6. The compound with the strongest protein binding ability was selected for visualization. The test results show that the active ingredients in the Chinese medicine composition provided by the present invention have a good theoretical basis for the treatment of sheep mycoplasma pneumonia infection.

[0068] Table 6: Molecular docking table

[0069]

[0070] Comparative Example 1: Preparation of Chinese medicine composition

[0071] The difference between this comparative example 1 and example 1 is that the Chinese medicine composition only includes the monarch drug, which is as follows:

[0072] Dry honeysuckle and ephedra separately, crush them, and pass them through a 50-mesh sieve. Then, add 30 grams of honeysuckle and 30 grams of ephedra to make the main medicine, add water to boil and concentrate, filter, discard the filter residue, and take the supernatant with a drug concentration of 1g / mL.

[0073] Comparative Example 2: Preparation of Chinese medicine composition

[0074] The difference between this comparative example 2 and example 1 is that the Chinese medicine composition only includes the auxiliary medicine, which is as follows:

[0075] Dried scutellaria baicalensis and astragalus separately, crushed, and passed through a 50-mesh sieve, 20 grams of scutellaria baicalensis and 20 grams of astragalus were mixed with the adjuvant, boiled and concentrated with water, filtered, the residue was discarded, and the supernatant with a drug concentration of 1g / mL was obtained.

[0076] Comparative Example 3:

[0077] The difference between this comparative example 3 and example 1 is that the Chinese medicine composition only includes adjuvant drugs and adjuvant drugs, which are as follows:

[0078] Dry and crush Fritillaria, Pinellia and Licorice separately, pass through a 50-mesh sieve, then mix 15 grams of Fritillaria and 15 grams of Pinellia with adjuvants and 20 grams of Licorice as a guiding drug, boil and concentrate with water, filter, discard the residue, and take the supernatant with a drug concentration of 1g / mL.

[0079] Test Example 1: Clinical Trial

[0080] In order to verify that the Chinese medicine composition of the present invention has a good therapeutic effect on sheep Mycoplasma pneumonia infection, adult sheep infected with sheep Mycoplasma pneumonia were selected for experiment.

[0081] 40 sheep infected with sheep mycoplasma pneumonia (symptoms: high fever, dyspnea, runny nose, cough) were randomly divided into test group 1, test group 2, test group 3 and control group, wherein test group 1 took 50 ml of the Chinese medicine composition prepared in Example 1, test group 2 took 50 ml of the Chinese medicine composition prepared in Comparative Example 1, test group 3 took 50 ml of the Chinese medicine composition prepared in Comparative Example 2, and control group took 50 ml of the Chinese medicine composition prepared in Comparative Example 3; each group of sheep took it once in the morning and evening. Take it for three consecutive days, observe the therapeutic effect, and calculate the therapeutic effectiveness by the formula = (effective number of sheep) / total number of sample sheep × 100%. The judgment criteria for the therapeutic effect are:

[0082] Effective: High fever symptoms no longer occur, and symptoms of dyspnea, runny nose and cough are relieved.

[0083] Ineffective: There is no significant improvement in the above symptoms, and some even get worse.

[0084] Table 7: Therapeutic effect on sheep mycoplasma pneumonia infection

[0085] Group efficient invalid Treatment effectiveness Test Group 1 10 0 100％ Test Group 2 7 3 70％ Experiment 3 groups 6 4 60％ Control group 6 4 60％

[0086] The experimental results show that the Chinese medicine composition provided by the present invention achieves the purpose of synergistic enhancement through the main drug, the auxiliary drug, the adjuvant drug and the guiding drug, and can effectively treat sheep mycoplasma disease infection.

[0087] The above description is only the preferred embodiment of the present application and is not intended to limit the present application. For those skilled in the art, the present application may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A Chinese medicine composition for treating mycoplasma diseases in livestock, characterized in that: The Chinese medicine composition is composed of the following raw materials in parts by weight: 30-50 parts of honeysuckle, 30-50 parts of ephedra, 20-35 parts of scutellaria, 20-35 parts of astragalus, 15-30 parts of fritillaria, 15-30 parts of pinellia, and 10-30 parts of liquorice.

2. The Chinese medicine composition according to claim 1, characterized in that: The Chinese medicine composition is composed of the following raw materials in parts by weight: 30 parts of honeysuckle, 30 parts of ephedra, 20 parts of scutellaria, 20 parts of astragalus, 15 parts of fritillaria, 15 parts of pinellia, and 20 parts of liquorice.

3. The Chinese medicine composition according to claim 1, characterized in that The mycoplasma disease is ovine mycoplasma pneumonia.

4. The method for preparing the Chinese medicine composition according to claim 1 or 2, characterized in that: The following steps are involved: Honeysuckle, ephedra, scutellaria, astragalus, fritillaria, pinellia and liquorice are dried, crushed and sieved respectively, mixed according to weight proportions, and boiled and concentrated with water to obtain a medicinal solution with a concentration of 1 g / mL.

5. Use of the Chinese medicine composition according to claim 1 or 2 in the preparation of a medicament for treating mycoplasma diseases in livestock.

6. A drug for treating mycoplasma disease in livestock, characterized in that: The medicine uses the Chinese medicine composition according to claim 1 or 2 as an active ingredient.

7. The drug for treating mycoplasma disease in livestock according to claim 6, characterized in that: The medicament may further include pharmaceutically acceptable excipients.

8. The drug for treating mycoplasma disease in livestock according to claim 7, characterized in that: The pharmaceutically acceptable excipients are disintegrants, lubricants, sweeteners or binders.

9. The drug for treating mycoplasma disease in livestock according to claim 8, characterized in that: The dosage form of the medicine is solution, soluble powder or injection.

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