Saline-alkali tolerant growth-promoting bacterium composition and application thereof
By optimizing the proportion of active ingredients and additive formula of saline-alkali-resistant bacterial promoters, a salt-alkali-resistant bacterial promoters is formed, which solves the problem of insufficient stability and efficacy of bacterial promoters in the saline-alkali land environment in the prior art, and achieves long-term stabilization effects and significantly improves crop growth performance.
Patent Information
- Application Number
- CN202510273313.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-03-10
- Publication Date
- 2025-04-15
- Estimated Expiration
- Not applicable · inactive patent
AI Technical Summary
The existing salt-alkali-resistant bacterial promoters have insufficient stability and efficacy in complex environments of saline-alkali land, mainly due to the imbalance in the proportion of active ingredients and poor adaptability of additives.
A bacterial promoter consisting of Z. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C. C
It significantly extends the effectiveness of bacterial agents, ensures that they play a stable role in saline-alkali land for a long time, improves the soil environment, and improves the germination rate, stress resistance and yield of crops.
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Figure CN119823920A_ABST
Abstract
Description
Technical Field
[0001] The invention relates to the technical field of agricultural microorganisms, and in particular to a salt-alkali tolerant growth-promoting bacteria composition and application thereof. Background Art
[0002] As a major obstacle to agricultural development, the improvement and utilization of saline-alkali land has always been a global research hotspot. Although salt-alkali-tolerant growth-promoting agents have potential, existing formulas have defects, such as unbalanced active ingredient ratios and poor adjuvant adaptability, which greatly reduce their stability and growth-promoting effectiveness in the complex environment of saline-alkali land. This study accurately locates these problems and explores formulation optimization strategies with innovative thinking, which has irreplaceable significance for promoting the development of saline-alkali land agriculture. Summary of the invention
[0003] In view of the above problems, the present invention provides a salt-alkali tolerant growth-promoting bacteria composition and application thereof.
[0004] The present invention provides a salt-alkali resistant growth-promoting bacteria composition, which is composed of a growth-promoting bacteria agent and an auxiliary agent; The growth-promoting bacteria agent is composed of Cynomolgus sinensis ( Planomicrobium chinense )、Glacier Zoomicrobacteria( Planomicrobium ice )、Lebito Springs Zoococcus( Planococcus rifietoensis ) composition; according to the number of viable bacteria, Sinococcus: Glacier Micrococcus: Lebito Springs Micrococcus = 3:2:1; The auxiliary agent consists of a protective agent, a binder and a carrier; the amount of the protective agent is 1.1% of the total mass of the growth-promoting bacteria agent, the amount of the binder is 4% of the total mass of the growth-promoting bacteria agent, and the amount of the carrier is 40% of the total mass of the growth-promoting bacteria agent.
[0005] Furthermore, the protective agent is ascorbic acid and thiourea; the amount of ascorbic acid used is 0.8% of the total mass of the growth-promoting bacteria agent, and the amount of thiourea used is 0.3% of the total mass of the growth-promoting bacteria agent.
[0006] This combination can effectively protect the bacterial strains from damage caused by the saline-alkali environment throughout the entire process of storage and use, significantly extend the effective period of the bacterial agent, and ensure its long-term and stable effect in saline-alkali land.
[0007] Furthermore, the binder is sodium carboxymethyl cellulose, which can promote the close attachment of the bacterial agent to the surface of the crop root system.
[0008] Furthermore, the carrier is vermiculite and diatomaceous earth, and the mass ratio of vermiculite to diatomaceous earth is 1:1.
[0009] Vermiculite and diatomaceous earth can improve the looseness and air permeability of the bacterial agent, creating ideal conditions for the bacterial strain to survive, spread in the soil and interact with the crop root system. Furthermore, in the culture medium of the three salt- and alkali-tolerant strains of the growth-promoting bacteria, namely, Sinococcus sinensis, Zoologica glaciosa, and Zoologica lebitovici, sucrose and mannitol are mixed in a ratio of 1:1 as a carbon source, potassium nitrate and urea are mixed in a ratio of 2:1 as a nitrogen source, potassium dihydrogen phosphate is used as a phosphorus source, and chelates of indole-3-acetic acid and trace elements are added.
[0010] Breaking through the limitation of using a single carbon source, it was discovered for the first time that when sucrose and mannitol were mixed in a 1:1 ratio as a carbon source, it could significantly accelerate strain growth, promote biomass accumulation, and greatly enhance strain metabolic activity under high saline-alkali conditions, providing strong energy support for its growth-promoting effect.
[0011] Potassium nitrate and urea are mixed in a ratio of 2:1 as a nitrogen source, and combined with potassium dihydrogen phosphate as a phosphorus source, which can perfectly meet the nutritional needs of the strain in a saline-alkali environment and effectively stimulate the strain to synthesize growth-related metabolites.
[0012] Plant growth regulators act directly on key growth nodes of crops, while trace elements enhance the strain's stress resistance and growth-promoting ability. The two work synergistically to create a good internal environment for crops to grow in saline-alkali land.
[0013] Furthermore, the concentration of indole-3-acetic acid is 0.3 mg / L, and the concentration of the trace element chelate is 0.03%; the trace elements include zinc, boron, and iron.
[0014] The present invention also provides an application of a salt-alkali tolerant growth-promoting bacteria composition in improving the soil environment of saline-alkali land and promoting the growth and development of crops in saline-alkali land.
[0015] Furthermore, the growth-promoting bacteria composition is applied in the following manner: spreading the growth-promoting bacteria composition into saline-alkali land and mixing seeds with the growth-promoting bacteria composition before sowing.
[0016] Furthermore, the spreading amount of the growth-promoting bacteria composition is 80 grams per square meter; the seed mixing ratio is a mass ratio of the growth-promoting bacteria composition to seeds of 1:8.
[0017] Compared with the prior art, the present invention has the following beneficial effects: In the present invention, a salt-alkali-tolerant bacterial strain with strong adaptability and growth-promoting potential in a high-salt-alkali environment is selected, the components of the bacterial strain culture medium are optimized, and then an adjuvant with good adaptability is added to obtain a salt-alkali-tolerant growth-promoting bacterial composition. On the one hand, the salt-alkali-tolerant growth-promoting bacterial composition of the present invention can improve the soil environment, including reducing soil salinity and improving soil structure, by reducing soil pH and salinity by producing organic acids through the bacterial strains in the bacterial agent, and by the interaction between the bacterial strains and soil particles and decomposition of organic matter; on the other hand, it can promote crop growth and development, including increasing seed germination rate, enhancing crop stress resistance, and increasing crop yield and quality, by providing a suitable microenvironment for seeds through the bacterial agent, inducing crops to produce stress resistance physiological reactions, and promoting root growth and development. BRIEF DESCRIPTION OF THE DRAWINGS
[0018] Figure 1 The effect of different ratios of the strain combinations of the present invention on the stem diameter; Figure 2 The effect of different ratios of bacterial strain combinations on root length of the present invention; Figure 3 The effect of different ratios of strain combinations of the present invention on plant height; Figure 4 The mixed bacterial solution OD of the present invention is 600 value; Figure 5 is the OD of the nitrogen source and phosphorus source ratio of the present invention 600 value; Figure 6 It is the effect of the combination of special ingredients in different ratios of the present invention on the stem diameter; Figure 7 It is the effect of the combination of special ingredients in different ratios of the present invention on root length; Figure 8 It is the influence of the combination of special ingredients in different ratios of the present invention on the plant height. DETAILED DESCRIPTION
[0019] In order to make the purpose and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.
[0020] The instruments, reagents, materials, etc. involved in the following examples, unless otherwise specified, are all conventional instruments, reagents, materials, etc. in the prior art and can be obtained through regular commercial channels. The experimental methods, detection methods, etc. involved in the following examples, unless otherwise specified, are all conventional experimental methods, detection methods, etc. in the prior art.
[0021] Example 1 This embodiment provides a salt-alkali tolerant growth-promoting bacteria composition, which is composed of a growth-promoting bacteria agent and an auxiliary agent; The growth-promoting agent is composed of Cynomolgus sinensis ( Planomicrobium chinense )、Glacier Zoomicrobacteria( Planomicrobium ice )、Lebito Springs Zoococcus( Planococcus rifietoensis ) composition; according to the number of viable bacteria, Sinococcus: Glacier Micrococcus: Lebito Springs Micrococcus = 3:2:1; The additives are composed of a protective agent, a binder, and a carrier; the protective agent is ascorbic acid and thiourea, and the dosage of ascorbic acid and thiourea is 0.8% and 0.3% of the total mass of the growth-promoting agent respectively; the binder is sodium carboxymethyl cellulose, and the dosage is 4% of the total mass of the growth-promoting agent; the carrier is vermiculite and diatomaceous earth with a mass ratio of 1:1, and the dosage is 40% of the total mass of the growth-promoting agent.
[0022] In the culture medium of the growth-promoting bacteria agent, sucrose and mannitol are mixed in a ratio of 1:1 as the carbon source, potassium nitrate and urea are mixed in a ratio of 2:1 as the nitrogen source, potassium dihydrogen phosphate is used as the phosphorus source, and indole-3-acetic acid at a concentration of 0.3 mg / L and chelates of trace elements such as zinc, boron, and iron at a concentration of 0.03% are added.
[0023] The present embodiment also provides an application of a salt-alkali tolerant growth-promoting bacteria composition in improving the soil environment of saline-alkali land and promoting the growth and development of crops in saline-alkali land. The application method is: spreading the growth-promoting bacteria composition into the saline-alkali land and mixing the seeds with the growth-promoting bacteria composition before sowing, with the spreading amount being 80 grams per square meter; the seed mixing ratio is a mass ratio of the growth-promoting bacteria composition to seeds of 1:8.
[0024] Experimental Example 1 Selection and combination of salt-alkali tolerant strains 1. Selection of salt-alkali tolerant strains Will purchase salt-alkali tolerant strains from the Chinese Academy of Sciences Institute of Microbiology Planomicrobium in Chinese Sinococcus, Planomicrobium ice Glacier swimming microbes, Planococcus from Rivieto Lebito Springs Mosinococcus, these strains show strong adaptability and growth-promoting potential in high-salt and alkaline environments.
[0025] 2. Determine the ratio of strain combination An innovative orthogonal experimental scheme was used (see Table 1) to set the strain Planomicrobium chinense Sinococcus, Planomicrobium ice Glacier swimming microbes, Planococcus rifietoensis Multiple combinations of different proportions of Lebito Springs Mosinococcus (see Table 1) were inoculated into culture media simulating saline-alkali soil environments (NaCl 12%, Na2CO3 1.5%), and corn seeds were added simultaneously. After 21 days of careful cultivation, the germination rate of corn seeds and key indicators such as stem diameter, root length, and plant height of seedlings were accurately measured (see Figure 1-3). Through in-depth data analysis, the strain was finally determined Planomicrobium chinense Sinococcus, Planomicrobium ice Glacier swimming microbes, Planococcus rifietoensis The optimal combination ratio of Lebito Springs Zoococcus is 3:2:1, which shows excellent effect in promoting corn seed germination and seedling growth.
[0026] Table 1 Orthogonal experimental plan
[0027] Example 2 Optimization of culture medium composition 1. Carbon source optimization The strains were cultured in a specific medium containing 10% NaCl and 1% Na2CO3 using glucose, sucrose, mannitol and their various combinations as carbon sources (see Table 2). Planomicrobium chinense Sinococcus, Planomicrobium ice Glacier swimming microbes, Planococcus rifietoensis Mixed bacterial solution of Lebito Springs Mosinococcus. With the help of advanced instruments and equipment, the OD of the bacterial solution cultured for 20 hours was accurately measured. 600 Value (see Figure 4 ) and biomass (see Table 2). The experimental results clearly showed that when sucrose and mannitol were mixed in a ratio of 1:1 as carbon sources, the strain had the best growth trend, the largest biomass accumulation, and the strongest metabolic activity.
[0028] Table 2 Carbon source optimization table
[0029] 2. Optimization of nitrogen and phosphorus sources Using potassium nitrate, urea, and ammonium sulfate as nitrogen sources, and potassium dihydrogen phosphate and diammonium hydrogen phosphate as phosphorus sources, a variety of different combinations were carefully designed for comparative experiments (see Table 3). Figure 5 ), potassium nitrate and urea are mixed in a ratio of 2:1 as a nitrogen source, and when combined with potassium dihydrogen phosphate as a phosphorus source, it can perfectly meet the nutritional needs of the strain in a saline-alkali environment. The strain grows the fastest in a saline-alkali environment and has the most significant growth-promoting effect, which can effectively promote the strain to synthesize a variety of key metabolites related to growth promotion.
[0030] Table 3 Ratio of nitrogen source and phosphorus source ,
[0031] 3. Add special ingredients: Different concentrations of plant growth regulator indole-3-acetic acid (IAA) and chelates of trace elements such as zinc, boron, and iron were added to the culture medium (see Table 4), and the strain's promotion effect on corn seed germination and seedling growth was carefully observed. After repeated experiments and optimization, it was finally determined that when the concentration of indole-3-acetic acid was 0.3 mg / L and the concentration of trace element chelates was 0.03%, the strain's promotion effect on corn seed germination (see Table 4) and seedling growth reached its peak (see Figure 6-8 ).
[0032] Table 4 Ratio of IAA and trace elements
[0033] Experimental Example 3 Selection of additives and determination of dosage 1. Protective agent In strains containing Planomicrobium chinense Sinococcus, Planomicrobium ice Glacier swimming microbes, Planococcus rifietoensis Different proportions of ascorbic acid and thiourea were added to the inoculum of the Lebito Springs Mosquito Coccus (see Table 5). After the inoculum was stored in a simulated saline-alkali environment for a period of time, the survival rate of the strains in the inoculum was accurately detected using advanced microbial detection technology. The experimental results showed that when the amount of ascorbic acid was 0.8% of the total mass of the inoculum and the amount of thiourea was 0.3%, the survival rate of the strain in the saline-alkali environment was the highest (see Table 5), which could effectively resist the damage of the saline-alkali environment to the strain and significantly extend the validity period of the inoculum.
[0034] Table 5 Protective agent ratio
[0035] 2. Binder and carrier Different proportions of sodium carboxymethyl cellulose were added to the microbial agent as a binder (see Table 6), and different mixing ratios of vermiculite and diatomaceous earth were used as carriers (see Table 6). The adhesion of the microbial agent on the surface of the crop root system was observed by a high-resolution microscope, and the diffusion of the microbial agent in the soil was simulated using a soil diffusion model. After a large number of experiments and data analysis, it was determined that when the amount of sodium carboxymethyl cellulose was 4% of the total mass of the microbial agent, and when vermiculite and diatomaceous earth were mixed in a ratio of 1:1 and the amount was 40% of the total mass of the microbial agent, the microbial agent had the best adhesion effect on the surface of the crop root system and the best diffusion performance in the soil (see Table 6), which could provide ideal physical support for the survival and diffusion of the strain in the soil and its interaction with the crop root system.
[0036] Table 6 Binder and carrier ratio
[0037] Experimental Example 4 Effect of salt-alkali tolerant growth-promoting bacteria composition on the growth of crops in saline-alkali land 1. Improve soil environment A typical saline-alkali land with an area of 500 square meters was carefully selected as the experimental site. The optimized salt-alkali tolerant growth-promoting bacteria composition was evenly spread on the surface of the experimental land at a precise dosage of 80 grams per square meter. Then, professional agricultural machinery was used to plow the land to a depth of 15-20 cm to ensure that the bacterial agent and the soil were fully and evenly mixed.
[0038] Soil samples were collected before, 3 months after, and 6 months after the application of the growth-promoting bacterial composition, and key indicators such as soil pH, salinity, and aggregate structure were measured using high-precision soil testing instruments. Experimental data showed that 6 months after the application of the bacterial agent, the soil pH value dropped significantly from the original 8.5-9.0 to 7.5-8.0, the salinity dropped significantly from 2.0%-2.5% to 1.5%-2.0%, the average particle size of soil aggregates increased from 0.2-0.25mm to 0.28-0.32mm, and the soil bulk density decreased by 0.1-0.2g / cm 3 , the overall soil quality has been significantly improved.
[0039] It can be seen that after applying the salt-alkali tolerant growth-promoting bacteria composition to saline-alkali land, the strain produces organic acids (citric acid, oxalic acid, etc.) through a unique metabolic pathway, and reacts chemically with the saline-alkali components of the soil to reduce the soil pH value and salinity, thereby improving the chemical properties of the soil from the source and creating a suitable acid-base environment for crop growth. In addition, during the growth and reproduction process of the strain, its mycelium forms a new interaction mode with secretions and soil particles, enhancing the stability of soil aggregates. At the same time, the strain efficiently decomposes soil organic matter, increases the humus content, significantly improves soil aeration and water permeability, reduces soil bulk density, builds a good soil physical structure, and promotes the free extension of crop roots in saline-alkali land.
[0040] 2. Promote crop growth and development Seed dressing treatment: Mix the corn seeds with the optimized salt-alkali-tolerant growth-promoting bacteria composition in a precise ratio of 8:1 (mass ratio), stir them thoroughly in professional mixing equipment, and then dry them for later use.
[0041] Sowing and growth monitoring: Sow the corn seeds after seed dressing in the above-mentioned improved saline-alkali land, and set up a control treatment area without seed dressing for comparative research. During the whole process of corn growth, professional measuring tools are used regularly to measure key growth indicators of corn such as plant height, leaf area, and root activity, and the data are recorded in detail. At the harvest time, advanced yield statistics equipment is used to count corn yields, and high-precision detection instruments are used to measure corn quality-related indicators. The experimental results show that the germination rate of corn seeds treated with seed dressing reached 78% (the control was 45%), the corn plant height was 22% higher than the control in the late growth period, the leaf area increased by 30%, and the root activity increased by 45%. The corn yield increased by 30%, the diameter of corn kernels increased by an average of 0.8 mm, and the yield per plant increased by 1.2%, which fully proved the significant promotion effect of the optimized microbial agent on the growth and development of corn.
[0042] It can be seen that by mixing the salt-alkali-tolerant growth-promoting bacteria composition with seeds before sowing, the strains create a unique microenvironment around the seeds, enhance the water absorption capacity of the seeds and the internal enzyme activity, and greatly increase the germination rate of seeds in saline-alkali land, laying a solid foundation for crop growth. In addition, throughout the growth of crops, the salt-alkali-tolerant growth-promoting bacteria composition establishes a new symbiotic relationship with the crop root system or efficiently colonizes in the rhizosphere. By activating the activity of crop antioxidant enzymes (superoxide dismutase, peroxidase, etc.), strengthening the stability of crop cell membranes, reducing intracellular electrolyte extravasation, and promoting root growth and development (increasing length, surface area and vitality), the tolerance of crops to salt-alkali stress is comprehensively improved, allowing crops to thrive in harsh saline-alkali environments.
[0043] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims
1. A salt-alkali tolerant growth-promoting bacteria composition, characterized in that: The growth-promoting bacteria composition is composed of a growth-promoting bacteria agent and an auxiliary agent; The growth-promoting bacteria agent is composed of Cynomolgus sinensis ( Planomicrobium chinense )、Glacier Zoomicrobes( Planomicrobium glaciei )、Lebito Springs Zoococcus( Planococcus rifietoensis ) composition; according to the number of viable bacteria, Sinococcus: Glacier Micrococcus: Lebito Springs Micrococcus = 3:2:1; The auxiliary agent consists of a protective agent, a binder and a carrier; the amount of the protective agent is 1.1% of the total mass of the growth-promoting bacteria agent, the amount of the binder is 4% of the total mass of the growth-promoting bacteria agent, and the amount of the carrier is 40% of the total mass of the growth-promoting bacteria agent.
2. The salt-alkali-tolerant growth-promoting bacteria composition according to claim 1, characterized in that: The protective agents are ascorbic acid and thiourea; the amount of ascorbic acid used is 0.8% of the total mass of the growth-promoting bacteria agent, and the amount of thiourea used is 0.3% of the total mass of the growth-promoting bacteria agent.
3. The salt-alkali-tolerant growth-promoting bacteria composition according to claim 1, characterized in that: The binder is sodium carboxymethyl cellulose.
4. The salt-alkali-tolerant growth-promoting bacteria composition according to claim 1, characterized in that: The carriers are vermiculite and diatomaceous earth, and the mass ratio of vermiculite to diatomaceous earth is 1:
1.
5. The salt-alkali-tolerant growth-promoting bacteria composition according to claim 1, characterized in that: In the culture medium of the three salt-alkali resistant strains of the growth-promoting bacteria, namely, Sino-Motile Coccus, Glacier Motile Coccus, and Lebito Spring Motile Coccus, sucrose and mannitol are mixed in a ratio of 1:1 as a carbon source, potassium nitrate and urea are mixed in a ratio of 2:1 as a nitrogen source, potassium dihydrogen phosphate is used as a phosphorus source, and indole-3-acetic acid and a chelate of trace elements are added.
6. The salt-alkali-tolerant growth-promoting bacteria composition according to claim 5, characterized in that: The concentration of indole-3-acetic acid is 0.3 mg / L, and the concentration of the trace element chelate is 0.03%; the trace elements include zinc, boron and iron.
7. Use of a salt-tolerant and growth-promoting bacteria composition as described in any one of claims 1 to 6 in improving the soil environment of saline-alkali land and promoting the growth and development of crops in saline-alkali land.
8. The use according to claim 7, characterized in that: The application method of the growth-promoting bacteria composition is: spreading the growth-promoting bacteria composition into saline-alkali land and mixing seeds with the growth-promoting bacteria composition before sowing.
9. The use according to claim 8, characterized in that: The spreading amount of the growth-promoting bacteria composition is 80 grams per square meter; the seed mixing ratio is a mass ratio of the growth-promoting bacteria composition to seeds of 1:8.