Method for analyzing mixing proportion of secondary mixing DNA by using SLSQP

Through the SLSQP analysis method, combined with PCR amplification and electrophoresis separation, the mixing ratio of DNA samples was optimized, which solved the inaccuracy and complexity of multi-donor sample analysis in traditional methods, and achieved efficient and automated genotype combination screening and result reliability.

CN119832990AActive Publication Date: 2025-04-15THE FIRST RES INST OF MIN OF PUBLIC SECURITY
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Patent Information

Application Number
CN202411876840.4
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-12-19
Publication Date
2025-04-15
Estimated Expiration
2044-12-19

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Abstract

The invention discloses a method for analyzing the mixing proportion of double-mixing DNA by using SLSQP. The method comprises the steps of data preparation, data preprocessing, genotype combination generation, genotype frequency after mixing, objective function definition and optimization. According to the method, the optimization method for minimizing the objective function is introduced, so that the proportion of each donor can be accurately estimated under the complex conditions of multiple genotypes and multiple donors, and the method does not need to depend on manual interpretation or a traditional comparison method. Besides, all possible genotype combinations are created and combinations meeting conditions are screened, so that the high efficiency of calculation is ensured, and the influence of invalid combinations on an analysis result is reduced. The method is not only suitable for simple diallele samples, but also can effectively process complex samples containing multiple alleles, and is wider in application range. According to the method, through automatic calculation and optimization, errors possibly caused by manual operation in a traditional method are avoided, and the repeatability and credibility of results are improved.
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