Method for characterizing the electro-taxis of plant roots and its applications

Abstract

The present application relates to the technical field of plant electric response, in particular to a method for characterizing the electric dual tropism of plant roots and application thereof, two metal plates are inserted into two sides of a cuboid water tank in parallel, the two metal plates are connected with the positive and negative poles of a direct current power supply respectively, a culture medium is added into the water tank to form a closed loop, seeds are placed on a sponge and covered with double-layer gauze, then the sponge is put into the culture medium in the water tank to culture the seeds; the voltage intensity applied to the two sides of the culture medium and the current intensity flowing through the culture medium are adjusted, so that the roots grown from the seeds grow towards the cathode and anode directions, thereby the electric dual tropism of the plant roots is characterized. The method is simple, easy to operate and convenient to observe, can clearly characterize the electric dual tropism of the plant roots, and can reflect the electric response conditions and electric response characteristics when the electric dual tropism occurs, and has a broad application prospect.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of plant electro-response, and particularly relates to a method for characterizing plant root system electro-biotaxis and application thereof. BACKGROUND

[0002] Electrotaxis is a tropic behavior of organisms to react to an external electric field, resulting in growth or migration. The phenomenon of roots growing along the direction of the electric field was first observed in the late 19th century, and since then, there have only been sporadic reports in the literature. In either case, the electrotaxis reported at present is unidirectional, and cannot reflect the range of electrical response and the characteristics of electrical response of organisms. At the same time, based on the fact that all cellular organisms have an electron transport chain, and all cellular organisms need to obtain electron donors and electron acceptors from the environment to survive, all cellular organisms should have a natural bidirectional electrotaxis to electron donors and electron acceptors. However, there is currently no method for characterizing this behavior of organisms. SUMMARY

[0003] The present application aims to provide a method for characterizing plant root system electro-biotaxis and application thereof.

[0004] The technical scheme is as follows:

[0005] The present application provides a method for characterizing plant root system electro-biotaxis, comprising the following steps:

[0006] S1, two metal plates are inserted into both sides of a cuboid water tank in parallel, the two metal plates are connected to the positive and negative poles of a direct current power supply respectively, a culture medium is added to the water tank to form a closed loop, a seed is placed on a sponge and covered with double-layer gauze, and then the sponge is placed in the culture medium in the water tank to culture the seed;

[0007] S2, the voltage intensity applied to both sides of the culture medium and the current intensity flowing through the culture medium are adjusted, so that the roots grown from the seed grow towards the cathode and anode directions, thereby characterizing the plant root system electro-biotaxis.

[0008] Further, the voltage intensity and current intensity adjusted in step S2 are the voltage intensity and current intensity that can be grown by the plant root system.

[0009] Further, the material of the metal plate is stainless steel.

[0010] Further, the material of the water tank has light transmittance, facilitating observation of the growth state of the plant root system.

[0011] Further, the material of the water tank is transparent organic plate.

[0012] The application also includes the use of any of the above-mentioned methods in the field of biology, including one or more of environmental biology, developmental biology, bioelectrochemistry, bioelectrophysiology, biological evolution.

[0013] Further, (1) by adjusting the voltage intensity applied on both sides of the culture medium, the voltage range of the plant root system growing towards the cathode direction is determined;

[0014] (2) Within the determined voltage range, any voltage is selected as a fixed voltage, and under the fixed voltage, the current intensity is adjusted by changing the conductivity of the culture medium, and the current range when the root system tends to the cathode and anode respectively is determined;

[0015] (3) The proportion of the number of roots of the plant root system in the two different directions of the cathode and the anode under different current intensities of the fixed voltage is counted, and a two-dimensional coordinate system capable of presenting the change of root tropism with the change of current is established based on the obtained data;

[0016] The two-dimensional coordinate system reflects the electrical response characteristics of the corresponding plant root system, and is applied to reflect the differences between the corresponding species of the plant root system and the growth and development status of the species.

[0017] Further, the x-axis of the two-dimensional coordinate system is the growth direction of the root, the positive value represents the root growing towards the anode direction, the negative value represents the root growing towards the cathode direction, and the numerical value of the x-axis represents the percentage of the number of roots of the plant root system in the direction, the y-axis represents the current size, and the values under the fixed voltage condition are connected, and the line reflects the degree of change of the root tropism with the change of the current under the same voltage condition.

[0018] Compared with the prior art, the method is simple, easy to operate and convenient to observe, can clearly characterize the electrical bimodal tropism of the plant root system, and can reflect the electrical response conditions and electrical response characteristics when the electrical bimodal tropism occurs, and has a wide application prospect in the fields of environmental biology, developmental biology, bioelectrochemistry, bioelectrophysiology, biological evolution, etc. BRIEF DESCRIPTION OF DRAWINGS

[0019] Figure 1 It is an experimental device used in an embodiment.

[0020] Figure 2 It is a plant root system electrical tropism result display graph under different currents in an embodiment.

[0021] Figure 3 It is the electrical response characteristics of the wheat root system. DETAILED DESCRIPTION

[0022] The technical solutions of the present application are further illustrated below in combination with specific examples. It should be understood that the following examples are only illustrative and explanatory of the present application and should not be interpreted as limiting the scope of protection of the present application. Any technology realized based on the above description of the present application is encompassed within the scope intended to be protected by the present application. Example 1

[0023] A method for characterizing the electro-phototropism of plant roots, comprising the following steps:

[0024] S1, two stainless steel plates are inserted into both sides of a cuboid transparent organic plate water tank in parallel, the two stainless steel plates are connected to the positive and negative poles of a direct current power supply respectively, distilled water is added into the water tank to form a closed loop, the seeds are placed on a sponge and covered with double-layer gauze, and then the sponge is placed into the distilled water in the water tank to culture the seeds;

[0025] S2, the voltage range in which the roots grown by the seeds exhibit cathodic tropism is determined by adjusting the voltage intensity applied to both sides of the culture medium;

[0026] S3, any voltage value in the above voltage range is selected, the conductivity of the culture medium is changed to adjust the current intensity at the voltage value, the current range A1 in which the roots grown by the seeds exhibit anodic tropism and the current range A2 in which the roots exhibit cathodic tropism are determined, and the current range between A1 and A2 is the current range in which the roots exhibit bi-tropism at the voltage value. Example 2

[0027] Voltage conditions for characterizing the cathodic tropism of wheat roots

[0028] The voltage conditions for characterizing the cathodic tropism of wheat roots are performed in the following steps:

[0029] (1) Refer to Figure 1 Wheat (Yannong 19) seeds are uniformly placed on 6 sponges with a thickness of 2 cm, and the adjacent seeds are spaced 1 cm apart. Double-layer gauze is used for covering, and the sponges are placed in 6 water tanks (30×10×10 cm). The culture medium of the 6 water tanks is 1L of distilled water.

[0030] (2) Two stainless steel plates (10×10×0.1 cm) are inserted into both ends of each water tank in parallel, and then connected to the anode and cathode of an electrophoresis apparatus respectively. The voltage of the water tank is controlled by the electrophoresis apparatus, and the set voltages of 5 water tanks are 1V, 5V, 10V, 20V and 30V respectively, and the remaining one treatment group is not electrified, serving as a control.

[0031] (3) After 6 days of dark culture at 20±0.1℃, the tropism of the roots is recorded:

[0032] The control group grew in the same number in the cathode direction and in the anode direction, and when the applied voltage was 1 V, the root growth direction was not affected; when the applied voltage was 5 V, 80% of the roots were observed to grow in the cathode direction; when the applied voltage was 10 V, all the roots were observed to grow in the cathode direction; when the applied voltage was 20 V, all the roots were observed to grow in the cathode direction, but the root growth was significantly inhibited; when the applied voltage was 30 V, the roots were severely inhibited and could not grow normally. Therefore, the voltage condition for the wheat root system to exhibit cathodic tropism behavior is 5-20 V. Example 3

[0033] Current range for characterizing the wheat root system to exhibit cathodic tropism and anodic tropism

[0034] The current range for characterizing the wheat root system to exhibit cathodic tropism and anodic tropism was carried out in the following steps:

[0035] (1) Place the wheat (Yannong 19) seeds evenly on 6 pieces of 2 cm thick sponge, with a 1 cm gap between adjacent seeds. Cover with double-layer gauze and place in 6 water tanks (30x10x10 cm). Prepare mixed solutions with different conductivity by changing the ratio of distilled water and tap water. The culture medium of the 6 water tanks is 1 L of distilled water, distilled water, mixed solution 1 of distilled water and tap water, mixed solution 2 of distilled water and tap water, mixed solution 3 of distilled water and tap water, and tap water, respectively.

[0036] (2) Insert two stainless steel plates (10x10x0.1 cm) parallel to the ends of each water tank, and then connect them to the anode and cathode of the electrophoresis apparatus, respectively. One treatment group with only distilled water is not electrified, serving as a control, and the other 5 groups are applied with a voltage of 10 V, with current intensities of 0.005 mA, 0.3 mA, 0.5 mA, 1 mA, and 2 mA, respectively.

[0037] (3) After 6 days of dark culture at 20±0.1℃, take photos and record the tropism of the roots.

[0038] The results show that the control group grew in the same number in the cathode direction and in the anode direction; see Figure 2In the treatment group with a current intensity of 0.005 mA, all roots were observed to grow towards the cathode; in the treatment group with a current intensity of 0.3 mA, 60% of the roots were observed to grow towards the cathode; in the treatment group with a current intensity of 0.5 mA, 80% of the roots were observed to grow towards the anode; in the treatment group with a current intensity of 1 mA, all roots were observed to grow towards the anode; and in the treatment group with a current intensity of 2 mA, all roots were observed to grow towards the anode, and root growth was significantly inhibited. Therefore, the current conditions for wheat roots to exhibit cathodic tendency behavior are greater than 0–0.3 mA (excluding 0), and the current conditions for exhibiting anodic tendency behavior are 0.5–2 mA. Under a voltage of 10 V, the electrical equilibrium point of wheat roots is between 0.3 and 0.5 mA. Example 4

[0039] Plotting the electrical response characteristics of wheat roots under 10V voltage conditions using a coordinate system

[0040] The following steps were taken to plot the coordinate system of the electrical response characteristics of wheat roots under a 10V voltage condition:

[0041] (1) Under the condition of 10V voltage, the proportion of wheat roots in different growth directions in different current cultivation environments is taken as the corresponding x-axis coordinate. The proportion of roots growing towards the anode direction is represented by positive values, and the proportion of roots growing towards the cathode direction is represented by negative values.

[0042] (2) The y-axis represents the corresponding current magnitude. Using Excel to plot the graph, add trend lines and fitting formulas. The trend curve of wheat roots changing with current under 10V voltage conditions is shown in the attached figure. Figure 3 As shown. The cathode direction fitting formula is: y = 4E-06x 3 + 0.0006x 2 + 0.036x + 1; The fitting formula for the anode direction is: y = 4E-06x 3 - 0.0005x 2 +0.0202x + 0.005.

[0043] This invention uses wheat as an example, but it is not limited to wheat and can also be applied to bean sprouts, rice, flowers, herbaceous plants, etc.

[0044] While specific embodiments of the present invention have been described above, those skilled in the art should understand that the specific embodiments described are merely illustrative and not intended to limit the scope of the invention. Modifications and variations made by those skilled in the art in accordance with the spirit of the invention should be covered within the scope of protection of the claims of the present invention.

Claims

1. A method for characterizing the electrobipotency of plant roots, characterized in that: Includes the following steps: S1, two metal plates are inserted parallel to each other on both sides of the rectangular water tank. The two metal plates are connected to the positive and negative terminals of the DC power supply, respectively. A culture medium is added to the water tank to form a closed loop. The seeds are placed on a sponge and covered with double gauze. Then the sponge is placed in the culture medium in the water tank to cultivate the seeds. S2, by adjusting the voltage intensity applied to both sides of the culture medium and the current intensity flowing through the culture medium, causes the roots of the seed to grow towards the cathode and anode, thereby characterizing the electrobipotency of plant roots.

2. The method for characterizing the electrobipotency of plant roots according to claim 1, characterized in that: The voltage and current intensity adjusted in step S2 are the voltage and current intensity that the plant roots can grow at.

3. The method for characterizing the electrobipotency of plant roots according to claim 1, characterized in that: The metal plate is made of stainless steel.

4. The method for characterizing the electrobipotency of plant roots according to claim 1, characterized in that: The water tank is made of a translucent material, which makes it easy to observe the growth status of plant roots.

5. The method for characterizing the electrobipotency of plant roots according to claim 4, characterized in that: The water tank is made of transparent organic board.

6. The application of the method according to any one of claims 1-5, characterized in that: Applications in the biological field, which includes one or more of environmental biology, developmental biology, bioelectrochemistry, bioelectrophysiology, and biological evolution.

7. The application according to claim 6, characterized in that: By adjusting the voltage intensity applied to both sides of the culture medium, the voltage range for plant roots to grow towards the cathode can be determined. Within the defined voltage range, any voltage is selected as a fixed voltage. Under the fixed voltage, the current intensity is adjusted by changing the conductivity of the culture medium to determine the current range when the roots tend towards the cathode and anode, respectively. The proportion of plant roots in two different directions (positive and negative) under different current intensities at a fixed voltage was statistically analyzed, and a two-dimensional coordinate system was established based on the obtained data to show the change of root directional tendency with current. The two-dimensional coordinate system reflects the electrical response characteristics of the corresponding plant root system and is used to reflect the differences between species corresponding to plant root systems and the growth and development status of species.

8. The application according to claim 7, characterized in that: The x-axis of the two-dimensional coordinate system represents the growth direction of the roots. Positive values ​​indicate that the roots grow towards the anode, while negative values ​​indicate that the roots grow towards the cathode. The values ​​on the x-axis represent the percentage of roots in that direction. The y-axis represents the magnitude of the current. Connecting the values ​​under a fixed voltage condition into a line reflects the degree of directional change in the roots as the current changes under the same voltage condition.

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