Rapid breeding method for virus-free healthy sweet potato seedlings and virus-free seedling domesticating and transplanting equipment

Abstract

The invention discloses a sweet potato virus-free healthy seedling rapid breeding method and virus-free seedling domestication and transplanting equipment, and belongs to the field of virus-free seedling domestication and transplanting. A virus-free seedling domestication transplanting device comprises a moving trolley and further comprises a bearing disc arranged on the upper side of the moving trolley; the guide rail is arranged on the upper side of the bearing disc; the sliding surface is arranged on the guide rail; the baffle is fixedly arranged at the lower end of the guide rail; the supporting rods are fixedly arranged between the bearing disc and the guide rail; the plurality of cultivation cups are arranged in the guide rail; the roots of the virus-free seedlings are cleaned through flowing water, so that excessive culture media can be prevented from being attached to the roots of the virus-free seedlings, the situation that leaves of the virus-free seedlings are intertwined due to the fact that the virus-free seedlings are taken out and washed in a unified mode at present can be avoided, and then the virus-free seedling transplanting efficiency can be improved to a certain degree.

Description

Technical Field

[0001] The invention relates to the technical field of virus-free seedling transplanting, in particular to a method for rapidly breeding virus-free healthy sweet potato seedlings and virus-free seedling acclimation and transplanting equipment. Background Art

[0002] In recent years, the number of newly emerged dangerous sweet potato viruses has increased year by year, causing the phenomenon of "seed degeneration", resulting in reduced production or even total loss of yield, which has a serious impact on the quality and yield of sweet potatoes. Therefore, the sweet potato seedlings need to be detoxified and domesticated. In the process of detoxification and domestication of sweet potato seedlings, the healthy seedlings after detoxification need to be transplanted into the substrate for domestication and propagation.

[0003] In the process of transplanting the virus-free healthy seedlings, the culture medium at the roots of the virus-free test tube seedlings needs to be cleaned first, and then they are transplanted uniformly.

[0004] At present, when transplanting virus-free seedlings, it is necessary to first take out the virus-free seedlings from the culture bottles, then clean them uniformly, and finally transport them to the insect-proof net room for planting. In the process of transplanting, on the one hand, there is no professional transplanting equipment for transplanting, and it depends on manual transportation. When transplanting, the virus-free seedlings need to be taken out from the culture bottles one by one for transplanting, which may affect the transplanting efficiency. On the other hand, the virus-free seedlings need to be taken out and cleaned manually. The cleaned virus-free seedlings may be crossed together, and they need to be separated when planting, which may also affect the efficiency of domestication and transplanting of the virus-free seedlings. Summary of the invention

[0005] The technical problem to be solved by the present invention is to overcome the deficiencies of the prior art and provide a method for rapidly breeding virus-free healthy sweet potato seedlings and virus-free seedling acclimatization and transplanting equipment that can overcome the above problems or at least partially solve the above problems.

[0006] In order to achieve the above object, the present invention adopts the following technical solutions:

[0007] A virus-free seedling acclimation and transplanting device comprises: a mobile trolley, and also comprises: a carrying plate, which is arranged on the upper side of the mobile trolley; a guide track, which is arranged on the upper side of the carrying plate; a sliding surface, which is arranged on the guide track; a baffle, which is fixedly arranged at the lower end of the guide track; a plurality of support rods, which are fixedly arranged between the carrying plate and the guide track; a plurality of cultivation cups, which are arranged in the guide track; a plurality of groups of communication ports, which are symmetrically opened at the lower side of the cultivation cup; a sealing component, which is arranged in the cultivation cup, and the sealing component is used to block the communication port. When the cultivation cup is placed When the mobile trolley is placed on the guide track, the closing component opens to release the blockage of the connecting port; the transfer barrel is arranged on the lower side of the carrying plate, and the open end of the transfer barrel corresponds to the lower end of the guide track; the limiting plate is fixedly arranged on one side of the baffle, and the limiting plate is arranged directly above the transfer barrel; the water supply component is arranged on the mobile trolley, and the water supply component is used to supply water to the upper end of the guide track; when clean water flows from the guide track, the clean water enters the culture cup along the connecting port to rinse the culture medium; the controller is arranged on the mobile trolley.

[0008] In order to facilitate water supply to the guide track, the water supply assembly further includes a circulation pump and a water tank fixedly mounted on the mobile trolley, a liquid extraction pipe is arranged between the circulation pump and the water tank, and a liquid supply pipe is arranged between the output end of the circulation pump and the upper end of the guide track.

[0009] In order to facilitate the collection of culture medium, further, a storage box is fixedly connected to the mobile cart, a first drain pipe is arranged between the storage box and the bottom of the transfer barrel, a second drain pipe is fixedly connected between the water storage tank and the bottom of the transfer barrel, and solenoid valves are arranged in the first drain pipe and the second drain pipe.

[0010] Furthermore, inclined surfaces are symmetrically arranged on both sides of the guide track, and a flow cavity is arranged on the lower side of the inclined surfaces.

[0011] Preferably, a three-way pipe is fixedly connected to the upper end of the guide track, the liquid inlet end of the three-way pipe is connected to the liquid supply pipe, and the liquid discharge end of the three-way pipe matches the flow chamber.

[0012] In order to facilitate the limiting of the cultivation cup, further, a limiting track is fixedly connected inside the guide track, recessed areas are symmetrically arranged on the cultivation cup, a protruding area is arranged between two of the recessed areas, the connecting port is connected to the recessed areas, and connecting grooves matching the protruding areas are symmetrically opened on the cultivation cup, and multiple guide angles are arranged on the outer side of the connecting grooves.

[0013] In order to improve the impact effect on the root system of the virus-free seedlings, further, a culture net barrel is fixedly connected to the culture cup, and the culture net barrel is arranged in the protruding area.

[0014] In order to facilitate the closure of the connecting port and the connecting groove, the closing component further includes a limiting ring threadedly connected in the cultivation cup, a limiting cavity is arranged between the limiting ring and the cultivation cup, a magnetic sealing ring is sealingly and slidingly connected in the limiting cavity, the magnetic sealing ring is used to seal the connecting port and the connecting groove, the upper end of the magnetic sealing ring is fixedly connected to a spring, and the lower side of the guide rail is fixedly connected to an electromagnetic block.

[0015] In order to improve the stability of the magnetic sealing ring and improve the sealing performance of the cultivation cup, preferably, a magnetic sealing plate is symmetrically fixedly connected to the magnetic sealing ring, a retaining ring matching the cultivation net bucket is fixedly connected to the magnetic sealing plate, sealing grooves are symmetrically opened on the protruding area, a sealing block matching the sealing groove is fixedly connected to the magnetic sealing plate, a sealing ring is arranged on the upper edge of the sealing block, and a cleaning groove is symmetrically opened on the sealing block.

[0016] A method for rapidly breeding virus-free healthy sweet potato seedlings mainly comprises the following steps:

[0017] Step 1: Select healthy potato tubers for germination, and then detoxify the stem tips;

[0018] Step 2: Inducing seedlings, peeling off the stem tip meristem combination for induction culture;

[0019] Step 3: Virus detection: molecular detection of viruses on sweet potato seedlings;

[0020] Step 4: Organize rapid propagation, cut the virus-free test tube seedlings that have passed the virus test into segments and propagate them in the culture room;

[0021] Step 5: Planting and domestication: Move the virus-free test tube seedlings to natural conditions for 3 days, and place the culture cup containing the virus-free test tube seedlings on the guide track;

[0022] Step 6: The sealing component is opened, and the culture medium in the culture cup flows out from the multiple connecting ports;

[0023] Step 7: supply water to the upper end of the guide track through the water supply assembly, the water flows from top to bottom along the guide track, and the water flows into the culture cup through the connecting port to clean the roots of the virus-free test tube seedlings;

[0024] Step 8: The staff pushes the mobile cart to the transplanting area, takes out the cultivation cup at the bottom and the virus-free test tube seedlings in the cultivation cup, and plants the virus-free test tube seedlings in the vermiculite matrix after disinfection and sterilization;

[0025] Step 9: When the seedlings reach 20 cm in length, start cutting them for propagation.

[0026] Compared with the prior art, the present invention provides a virus-free seedling acclimatization and transplanting device, which has the following beneficial effects:

[0027] 1. The virus-free seedling acclimation and transplanting equipment places multiple cultivation cups in a guide track. When the cultivation cups are placed on the guide track, the internal sealing components are opened to release the blocking of the connecting port at the lower side of the cultivation cups. The circulating pump is turned on by the controller, and the circulating pump supplies water in the water storage tank to the upper end of the guide track. The water flows from top to bottom. At this time, the water flowing from top to bottom enters the cultivation cups through the connecting port to rinse the roots of the virus-free seedlings in the cultivation cups. The water after rinsing the roots of the virus-free seedlings will be discharged from the connecting port on the other side and flow downward along the guide track. Finally, the water enters the water storage tank again through the transfer barrel and the second drain pipe, thereby preventing the roots of the virus-free seedlings from adhering to too much culture medium.

[0028] 2. With the virus-free seedling acclimation and transplanting equipment, the staff can perform the same operation on other virus-free seedlings, thereby improving the cleaning efficiency of the roots of the virus-free seedlings and avoiding the entanglement of the leaves of the virus-free seedlings caused by uniformly taking out and washing the virus-free seedlings, thereby improving the efficiency of transplanting the virus-free seedlings to a certain extent.

[0029] 3. The virus-free seedling acclimatization and transplanting equipment can continuously supply water to the roots of the virus-free seedlings through the continuous circulation of water flow, thereby preventing the seedlings from wilting during large-scale transplanting.

[0030] 4. The virus-free seedling acclimation and transplanting equipment arranges a culture net barrel in the protruding area and places the virus-free seedlings in the culture net barrel. The culture net barrel can limit the roots of the virus-free seedlings to prevent the virus-free seedlings from tipping and shaking in the cultivation cup, and the roots of the virus-free seedlings are lifted to a certain height through the protruding area so that the roots of the virus-free seedlings contact with the upper surface of the water flow. At this time, the water flow will splash after colliding with the roots of the virus-free seedlings, thereby effectively improving the cleaning effect of the virus-free seedlings.

[0031] The parts not involved in the device are the same as the existing technology or can be implemented by the existing technology. The present invention cleans the roots of the virus-free seedlings with flowing water, thereby preventing too much culture medium from adhering to the roots of the virus-free seedlings, and avoiding the current situation of taking out the virus-free seedlings and washing them uniformly, which causes the leaves of the virus-free seedlings to be entangled with each other, thereby improving the efficiency of transplanting the virus-free seedlings to a certain extent. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] Figure 1 The structure of the present invention is schematically shown Figure 1 ;

[0033] Figure 2 The structure of the present invention is schematically shown Figure 2 ;

[0034] Figure 3 The structure of the present invention is schematically shown Figure 3 ;

[0035] Figure 4 The structure of the guide rail in the present invention is shown in FIG. Figure 1 ;

[0036] Figure 5 The structure of the guide rail in the present invention is shown in FIG. Figure 2 ;

[0037] Figure 6 The structure of the guide rail in the present invention is shown in FIG. Figure 3 ;

[0038] Figure 7 For the present invention Figure 6 The enlarged schematic diagram of point A in the middle;

[0039] Figure 8 It is a schematic diagram of the structure of the cultivation cup in the present invention;

[0040] Fig. 9 This is a schematic diagram of the expanded structure of the cultivation cup of the present invention;

[0041] Fig.10 It is a cross-sectional schematic diagram of the cultivation cup of the present invention.

[0042] In the figure: 1. Mobile trolley; 101. Carrying plate; 102. Support rod; 103. Transfer barrel; 104. First liquid discharge pipe; 105. Storage box; 106. Second liquid discharge pipe; 107. Water storage tank; 108. Circulation pump; 109. Liquid extraction pipe; 110. Liquid supply pipe; 111. Three-way pipe; 112. Connecting pipe; 113. Controller; 2. Guide rail; 201. Sliding surface; 202. Inclined surface; 203. Flow chamber; 204. Electromagnetic block; 205. Limiting rail; 206. Water spray pipe; 207, water spray port; 208, baffle; 209, flow limiting plate; 3, culture cup; 301, limiting ring; 302, limiting cavity; 303, recessed area; 304, connecting port; 305, connecting groove; 306, guide angle; 307, protruding area; 308, magnetic sealing ring; 309, spring; 4, culture net bucket; 401, magnetic sealing plate; 402, baffle ring; 403, sealing block; 404, cleaning tank; 405, collecting cavity; 406, guide tube; 407, sealing ring. DETAILED DESCRIPTION

[0043] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0044] Example 1: Reference Figure 1-Figure 7, a virus-free seedling domestication and transplanting device, comprising: a mobile trolley 1, and also comprising: a carrier plate 101, which is arranged on the upper side of the mobile trolley 1; a guide track 2, which is arranged on the upper side of the carrier plate 101; a sliding surface 201, which is arranged on the guide track 2; a baffle 208, which is fixedly arranged at the lower end of the guide track 2; a plurality of support rods 102, which are fixedly arranged between the carrier plate 101 and the guide track 2; a plurality of culture cups 3, which are arranged in the guide track 2; a plurality of groups of communication ports 304, which are symmetrically opened on the lower side of the culture cup 3; a sealing component, which is arranged in the culture cup 3, and the sealing component is used to block the communication port 304, when the culture cup 3 is placed When it is placed on the guide track 2, the closing component opens to release the blockage of the connecting port 304; the transfer barrel 103 is arranged on the lower side of the carrier plate 101, and the open end of the transfer barrel 103 corresponds to the lower end of the guide track 2; the limiting plate 209 is fixedly arranged on one side of the baffle 208, and the limiting plate 209 is arranged directly above the transfer barrel 103; the water supply component is arranged on the mobile cart 1, and the water supply component is used to supply water to the upper end of the guide track 2; when the clean water flows from the guide track 2, the clean water enters the culture cup 3 along the connecting port 304 to rinse the culture medium; the controller 113 is arranged on the mobile cart 1.

[0045] The water supply assembly includes a circulation pump 108 and a water tank 107 fixedly mounted on the mobile vehicle 1 , a liquid extraction pipe 109 is disposed between the circulation pump 108 and the water tank 107 , and a liquid supply pipe 110 is disposed between the output end of the circulation pump 108 and the upper end of the guide track 2 .

[0046] A storage box 105 is fixedly connected to the mobile cart 1, a first drain pipe 104 is arranged between the storage box 105 and the bottom of the transfer barrel 103, a second drain pipe 106 is fixedly connected between the water storage tank 107 and the bottom of the transfer barrel 103, and solenoid valves are arranged in the first drain pipe 104 and the second drain pipe 106.

[0047] During the process of acclimating and transplanting virus-free seedlings, a plurality of cultivation cups 3 are first placed in the guide track 2. When the cultivation cups 3 are placed on the guide track 2, the sealing components inside the cultivation cups 3 are opened to release the blockage of the connecting port 304 on the lower side of the cultivation cups 3. The circulating pump 108 is turned on by the controller 113. The circulating pump 108 supplies water in the water storage tank 107 to the upper end of the guide track 2. The water flows from top to bottom. At this time, the water flowing from top to bottom enters the cultivation cups 3 through the connecting port 304 to rinse the roots of the virus-free seedlings in the cultivation cups 3. The water after rinsing the roots of the virus-free seedlings will be discharged from the connecting port 304 on the other side and flow downward along the guide track 2. Finally, the water enters the water storage tank 107 again through the transfer barrel 103 and the second drainage pipe 106, thereby preventing the roots of the virus-free seedlings from adhering to too much culture medium.

[0048] During this cleaning process, the staff can perform the same operation on other virus-free seedlings, thereby improving the cleaning efficiency of the roots of the virus-free seedlings and avoiding the current situation of taking out the virus-free seedlings for unified washing, which causes the leaves of the virus-free seedlings to be entangled with each other, thereby improving the efficiency of transplanting the virus-free seedlings to a certain extent.

[0049] During the specific transplanting process, the mobile cart 1 is pushed to the transplanting area, wherein this transplanting step is to transplant the virus-free seedlings into the insect-proof net room. The staff can take out the virus-free seedlings and the cultivation cups 3 at the same position by standing at the lower end of the guide track 2, thereby effectively improving the efficiency of transplanting the virus-free seedlings.

[0050] Through the continuous circulation of water, the roots of the virus-free seedlings can be continuously supplied with water, preventing the phenomenon of wilting seedlings during large-scale transplanting.

[0051] After the cultivation cup 3 at the bottom is taken out, the cultivation cup 3 can be moved downward to the baffle 208 under the impact of the water flow and the action of the guide track 2 to prevent the cup from getting stuck.

[0052] Example 2: Reference Figure 1-Figure 10 , a virus-free seedling acclimatization and transplanting device, which is basically the same as Example 1, further, inclined surfaces 202 are symmetrically arranged on both sides of the guide track 2, and a flow cavity 203 is arranged on the lower side of the inclined surface 202.

[0053] The upper end of the guide track 2 is fixedly connected with a three-way pipe 111 , the liquid inlet end of the three-way pipe 111 is connected with the liquid supply pipe 110 , and the liquid discharge end of the three-way pipe 111 matches with the flow chamber 203 .

[0054] By setting two inclined surfaces 202 and a flow cavity 203 on the guide track 2, splashing of water can be avoided under certain water flow velocity and liquid level height. At this time, the water on both sides encounters less resistance and flows faster, so it will maintain a certain impact force when impacting the connecting port 304.

[0055] The guide track 2 is fixedly connected to the limited position track 205, the cultivation cup 3 is symmetrically provided with recessed areas 303, a protruding area 307 is provided between the two recessed areas 303, the connecting port 304 is connected with the recessed areas 303, the cultivation cup 3 is symmetrically provided with connecting grooves 305 matching the protruding areas 307, and a plurality of guide angles 306 are provided on the outer side of the connecting grooves 305.

[0056] By setting the limiting track 205, the cultivation cup 3 can be limited so that the connecting port 304 and the connecting groove 305 on the cultivation cup 3 always face the impact direction of the water flow, thereby preventing the cultivation cup 3 from rotating and affecting the cleaning effect on the roots of the virus-free seedlings.

[0057] The diversion angle 306 can guide the water so that the water flows through the connecting groove 305 to a greater extent to impact the roots of the virus-free seedlings.

[0058] Example 3: Reference Figure 1-Figure 10 , a virus-free seedling acclimatization and transplanting device, is basically the same as Example 1, and further, a culture net barrel 4 is fixedly connected to the culture cup 3, and the culture net barrel 4 is arranged in the protruding area 307.

[0059] A culture net barrel 4 is set in the protruding area 307, and the virus-free seedlings are placed in the culture net barrel 4. The culture net barrel 4 can limit the roots of the virus-free seedlings to prevent the virus-free seedlings from tipping over and shaking in the culture cup 3, and the roots of the virus-free seedlings are lifted to a certain height through the protruding area 307 so that the roots of the virus-free seedlings contact with the upper surface of the water flow. At this time, the water flow will splash after colliding with the roots of the virus-free seedlings, thereby effectively improving the cleaning effect of the virus-free seedlings.

[0060] The closing component includes a limiting ring 301 threadedly connected in the cultivation cup 3, a limiting cavity 302 is arranged between the limiting ring 301 and the cultivation cup 3, a magnetic sealing ring 308 is sealingly and slidingly connected in the limiting cavity 302, the magnetic sealing ring 308 is used to seal the connecting port 304 and the connecting groove 305, the upper end of the magnetic sealing ring 308 is fixedly connected to a spring 309, and the lower side of the guide track 2 is fixedly connected to an electromagnetic block 204.

[0061] When the electromagnetic block 204 is energized through the controller 113, the electromagnetic block 204 generates a magnetic force that repels the magnetic pole at the lower end of the magnetic sealing ring 308, so that the magnetic sealing ring 308 slides upward under the action of the magnetic repulsion, thereby releasing the limit on the connecting port 304 and the connecting groove 305. The operation is simple and effectively improves the cleaning effect of the virus-free seedlings and the transplanting efficiency of the virus-free seedlings.

[0062] A magnetic sealing plate 401 is symmetrically fixedly connected to the magnetic sealing ring 308, a retaining ring 402 matching the culture net bucket 4 is fixedly connected to the magnetic sealing plate 401, sealing grooves are symmetrically opened on the protruding area 307, a sealing block 403 matching the sealing groove is fixedly connected to the magnetic sealing plate 401, a sealing ring 407 is arranged on the upper edge of the sealing block 403, and a cleaning groove 404 is symmetrically opened on the sealing block 403.

[0063] In the specific implementation, in order to ensure the sealing of the cultivation cup 3, the sealing is achieved by the sealing ring 407 on the upper edge of the sealing block 403, and the magnetic sealing ring 308 can be limited at the same time. When the cultivation cup 3 needs to be placed on the guide track 2, the cultivation cup 3 is manually placed on the guide track 2, and the cultivation cup 3 is pressed downward. The limiting track 205 squeezes the sealing block 403 and moves it upward, so that the resistance of the magnetic sealing ring 308 is reduced. At this time, the magnetic sealing ring 308 can be driven upward by the magnetic force of the electromagnetic block 204, and the water entering the cultivation cup 3 through the connecting groove 305 flushes the roots of the virus-free seedlings through the cleaning groove 404.

[0064] The magnetic poles of the magnetic sealing plate 401 and the magnetic sealing ring 308 are the same.

[0065] Example 4: Reference Figure 1-Figure 10 , a virus-free seedling acclimation and transplanting device is basically the same as Example 1, and further, a collecting chamber 405 is opened on the lower side of the sealing block 403, and the collecting chamber 405 is in contact with the upper surface of the limiting track 205, a guide tube 406 matching the culture net bucket 4 is arranged in the sealing block 403, a water spray pipe 206 is arranged in the limiting track 205, and a plurality of water spray ports 207 connected with the water spray pipe 206 are arranged on the upper side of the limiting track 205, and the water spray ports 207 match the collecting chamber 405, a connecting pipe 112 is arranged between the water spray pipe 206 and the three-way pipe 111, and electronic flow valves are arranged at the water outlet ends of the connecting pipe 112 and the three-way pipe 111.

[0066] Part of the water can be guided into the water spray pipe 206 through the connecting pipe 112, and then sprayed out through multiple water spray ports 207. The water sprayed out from the water spray ports 207 enters the collecting chamber 405, and then sprayed out through the guide pipe 406. The sprayed water flushes the virus-free seedlings from top to bottom, thereby effectively improving the flushing effect of the virus-free seedlings, and further effectively improving the survival rate of the virus-free seedlings after transplanting.

[0067] Embodiment 5: A method for rapid breeding of virus-free healthy sweet potato seedlings, mainly comprising the following steps:

[0068] Step 1: Select healthy tubers for germination, and then detoxify the stem tips. In the specific operation, select healthy tubers that fully meet the characteristics of the variety and germinate them at 30-35 degrees Celsius. Take a 0.5 cm bud tip, first wash it with detergent and appropriate amount of clean water for 15-20 minutes, then rinse it with running water, soak it in 70% alcohol for 30 seconds in a clean bench, then treat it with 1.5% sodium hypochlorite for 15-20 minutes, and then wash it with sterile water;

[0069] Step 2, inducing seedlings, peeling the stem apex meristem combination for induction culture. In the specific operation, the cleaned and sterilized material is peeled under a biological microscope to obtain a 0.1-0.3 mm stem apex meristem combination for induction culture. The stem apex culture medium is based on MS culture medium, supplemented with 2.0 mg / L 6-BA and 0.2 mg / L NAA, the culture room temperature is controlled between 25-27 degrees Celsius, the light intensity is 2000Lx-2500Lx, the light time is 12-14 hours, and after 20-30 days of culture, it is transferred to MS culture medium. After 60-90 days of culture, seedlings with two or three leaves are obtained;

[0070] Step 3, virus detection, molecular detection of viruses on sweet potatoes, wherein the test tube seedlings of each strain are subjected to molecular detection of six major sweet potato viruses, including sweet potato feathery mottle virus SPFMV, sweet potato chlorotic stunt virus SPCSV, sweet potato leaf curl virus SPLCV, sweet potato G virus SPVG, sweet potato C virus SPVC, and sweet potato latent virus SPLV;

[0071] Step 4, tissue rapid propagation, the virus-free test tube seedlings that have passed the virus test are cut into segments and propagated in the culture room. When the test tube seedlings grow to 5 leaves, the virus-free test tube seedlings that have passed the virus test are cut into segments and propagated in the culture room using MS culture medium. When cutting, two axillary buds are left in each segment, one of which is inserted into the culture medium, and the subculture is performed once every 40-50 days, and the number of subcultures does not exceed 10 generations. During subculture and propagation, the temperature of the tissue culture room is slightly higher than the temperature for inducing seedlings;

[0072] Step 5: Planting and domestication: The virus-free test tube seedlings are cultivated for about 50 days. When the seedlings are more than 5 cm tall, the virus-free test tube seedlings are moved to natural conditions for 3 days, and the cultivation cup 3 containing the virus-free test tube seedlings is placed on the guide track 2;

[0073] Step 6: The sealing component is opened, and the culture medium in the culture cup 3 flows out from the multiple communication ports 304;

[0074] Step 7: water is supplied to the upper end of the guide track 2 through the water supply assembly, and the water flows from top to bottom along the guide track 2, and the water flows into the culture cup 3 through the connecting port 304 to clean the roots of the virus-free test tube seedlings;

[0075] Step 8, the staff pushes the mobile trolley 1 to the transplanting area, takes out the bottom cultivation cup 3 and the virus-free test tube seedlings in the cultivation cup 3, plants the virus-free test tube seedlings in the vermiculite matrix after disinfection and sterilization, and domesticates them in a 60-mesh insect-proof net room, hangs a yellow sticky insect board for every 5 square meters in the net room, waters them after planting, shades them with a sunshade net for a week, and then grows them under normal light;

[0076] Step nine, when the seedlings reach 20 cm in length, start cutting the seedlings for propagation. Pay attention to the disinfection and cleaning of scissors and other tools before each cutting. The seedlings are 15-20 cm long. Depending on the growth of the potato seedlings, cut the seedlings and propagate once every 10-15 days. Supplement water and fertilizer the next day after each cutting. The fertilizer is mainly nitrogen fertilizer. The domestication of the virus-free test tube seedlings is generally carried out in May-June and September-October. Pay attention to taking cooling measures during the high temperature stage in summer, and control the temperature within 30 degrees Celsius. The virus-free seedlings that have completed the domestication and propagation in May and June can be planted in the open field net room for propagation in July, produce virus-free seed potatoes, and supply field seedlings to the southern market in autumn. The virus-free test tube seedlings that have completed the domestication and propagation in September-October are planted in the warm greenhouse for overwintering and propagation to ensure the seedlings for the cold greenhouse and spring potatoes in March and April of the following year. The planting in the warm greenhouse is generally completed before December to improve the potato seedlings' ability to resist the cold over the winter.

[0077] The above are only preferred specific implementation modes of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical solutions and inventive concepts of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A virus-free seedling acclimatization and transplanting device, comprising: The mobile vehicle (1) is characterized by further comprising: A carrying plate (101) is arranged on the upper side of the moving vehicle (1); A guide track (2) arranged on the upper side of the carrying plate (101); A sliding surface (201) is arranged on the guide track (2); A baffle (208) fixedly arranged at the lower end of the guide track (2); A plurality of support rods (102) fixedly arranged between the carrying plate (101) and the guide track (2); A plurality of culture cups (3) are arranged in the guide track (2); A plurality of communication ports (304) are symmetrically arranged on the lower side of the culture cup (3); a sealing component, which is arranged in the cultivation cup (3) and is used to seal the communication port (304); when the cultivation cup (3) is placed on the guide track (2), the sealing component is opened to release the sealing of the communication port (304); A transfer barrel (103) is arranged on the lower side of the carrying plate (101), and the opening end of the transfer barrel (103) corresponds to the lower end of the guide track (2); A flow limiting plate (209) is fixedly arranged on one side of the baffle (208), and the flow limiting plate (209) is arranged directly above the transfer barrel (103); A water supply component, arranged on the mobile vehicle (1), the water supply component being used to supply water to the upper end of the guide track (2); When the clean water flows from the guide track (2), the clean water enters the culture cup (3) along the connecting port (304) to rinse the culture medium; A controller (113) is arranged on the mobile vehicle (1).

2. The virus-free seedling acclimatization and transplanting equipment according to claim 1 is characterized in that: The water supply assembly comprises a circulation pump (108) and a water storage tank (107) fixedly mounted on the mobile vehicle (1), a liquid extraction pipe (109) being arranged between the circulation pump (108) and the water storage tank (107), and a liquid supply pipe (110) being arranged between the output end of the circulation pump (108) and the upper end of the guide track (2).

3. The virus-free seedling acclimatization and transplanting equipment according to claim 2 is characterized in that: A storage box (105) is fixedly connected to the mobile trolley (1), a first liquid discharge pipe (104) is arranged between the storage box (105) and the bottom of the transfer barrel (103), a second liquid discharge pipe (106) is fixedly connected between the water storage tank (107) and the bottom of the transfer barrel (103), and electromagnetic valves are arranged in the first liquid discharge pipe (104) and the second liquid discharge pipe (106).

4. The virus-free seedling acclimatization and transplanting equipment according to claim 2, characterized in that: Inclined surfaces (202) are symmetrically arranged on both sides of the guide track (2), and a flow cavity (203) is arranged on the lower side of the inclined surfaces (202).

5. The virus-free seedling acclimatization and transplanting equipment according to claim 4 is characterized in that: The upper end of the guide track (2) is fixedly connected to a three-way pipe (111), the liquid inlet end of the three-way pipe (111) is connected to the liquid supply pipe (110), and the liquid discharge end of the three-way pipe (111) matches the flow chamber (203).

6. The virus-free seedling acclimatization and transplanting equipment according to claim 4 is characterized in that: The guide track (2) is fixedly connected to a limited track (205), the cultivation cup (3) is symmetrically provided with recessed areas (303), a protruding area (307) is provided between the two recessed areas (303), the connecting port (304) is connected to the recessed areas (303), the cultivation cup (3) is symmetrically provided with connecting grooves (305) matching the protruding areas (307), and a plurality of guide angles (306) are provided on the outer side of the connecting grooves (305).

7. The virus-free seedling acclimatization and transplanting equipment according to claim 6 is characterized in that: A culture net barrel (4) is fixedly connected to the culture cup (3), and the culture net barrel (4) is arranged in the protruding area (307).

8. The virus-free seedling acclimatization and transplanting equipment according to claim 7, characterized in that: The sealing component comprises a limiting ring (301) threadedly connected in the cultivation cup (3); a limiting cavity (302) is arranged between the limiting ring (301) and the cultivation cup (3); a magnetic sealing ring (308) is sealingly and slidably connected in the limiting cavity (302); the magnetic sealing ring (308) is used to seal the communication port (304) and the communication groove (305); a spring (309) is fixedly connected to the upper end of the magnetic sealing ring (308); and an electromagnetic block (204) is fixedly connected to the lower side of the guide track (2).

9. The virus-free seedling acclimatization and transplanting equipment according to claim 8, characterized in that: The magnetic sealing ring (308) is symmetrically fixedly connected to a magnetic sealing plate (401), the magnetic sealing plate (401) is fixedly connected to a retaining ring (402) matching the culture net bucket (4), the protruding area (307) is symmetrically provided with sealing grooves, the magnetic sealing plate (401) is fixedly connected to a sealing block (403) matching the sealing groove, a sealing ring (407) is arranged on the upper edge of the sealing block (403), and a cleaning groove (404) is symmetrically provided on the sealing block (403).

10. A method for rapid propagation of virus-free healthy sweet potato seedlings, using the virus-free seedling acclimatization and transplanting equipment according to claim 1, characterized in that: The main steps include: Step 1: Select healthy potato tubers for germination, and then detoxify the stem tips; Step 2: Inducing seedlings, peeling off the stem tip meristem combination for induction culture; Step 3: Virus detection: molecular detection of viruses on sweet potato seedlings; Step 4: Organize rapid propagation, cut the virus-free test tube seedlings that have passed the virus test into segments and propagate them in the culture room; Step 5: Planting and domestication: move the virus-free test tube seedlings to natural conditions for 3 days, and place the culture cup (3) containing the virus-free test tube seedlings on the guide track (2); Step 6: The sealing component is opened, and the culture medium in the culture cup (3) flows out from the multiple communication ports (304); Step 7: water is supplied to the upper end of the guide track (2) through the water supply assembly, the water flows from top to bottom along the guide track (2), and the water flows into the culture cup (3) through the connecting port (304) to clean the roots of the virus-free test tube seedlings; Step 8: The staff pushes the mobile trolley (1) to the transplanting area, takes out the cultivation cup (3) at the bottom and the virus-free test tube seedlings in the cultivation cup (3), and plants the virus-free test tube seedlings in the vermiculite matrix after disinfection and sterilization; Step 9: When the seedlings reach 20 cm in length, start cutting them for propagation.

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