Calcium nutritional supplement composition and bionic preparation method thereof

Abstract

The invention relates to the technical field of calcium nutritional supplements, in particular to a calcium nutritional supplement composition and a bionic preparation method thereof. The calcium nutritional supplement composition comprises the following components in percentage: 20-30% of lysine, 10-20% of calcium carbonate, 20-30% of whey protein, 20-30% of branched chain amino acid, 10-20% of glutamine, 0.3-0.4% of mineral elements and the balance of water. According to the technical scheme, the adopted materials are common raw materials for dietary supplements, whey protein, branched chain amino acid and calcium carbonate, are applied to formulas of milk powder, nutritional supplements and the like at high frequency, and are different from raw material sources which are required by other formulas and are difficult to widely apply, so that the purpose of promoting calcium absorption is achieved; and the material combination adopted by the technical scheme solves the application defects caused by single use of the combined material or combination of the combined material and other materials, converts low-cost inorganic calcium into organic calcium, significantly promotes absorption and utilization of calcium, also improves the sensory experience of eating, and significantly reduces the overall sensitization of the formula.

Description

Technical Field

[0001] The present invention relates to the technical field of calcium nutritional supplements, and in particular to a calcium nutritional supplement composition and a bionic preparation method thereof. Background Art

[0002] At present, the raw materials of calcium supplement products on the market mainly include: organic calcium: such as calcium gluconate, calcium lactate, calcium acetate, calcium citrate, calcium fructose, etc. Organic calcium has high solubility and less irritation to the gastrointestinal tract, but the calcium content is low, and the absorption rate is about 10-15%; inorganic calcium: such as calcium carbonate, calcium phosphate, calcium oxide, calcium hydrogen phosphate, etc., the absorption rate is 30%-40%. Inorganic calcium has low solubility and greater irritation to the gastrointestinal tract, but the calcium content is relatively high; active calcium: active calcium is made from animal bones and shells, and is converted by high-temperature calcination and electrolysis, mainly calcium hydroxide and calcium chloride. Although active calcium has a high degree of ionization and high solubility, its bioavailability is not high and the calcium content is small. Its small-fold solution is strongly alkaline (pH>12), with high acute toxicity and strong gastrointestinal irritation, which can easily cause nausea and vomiting. Calcium products contain high levels of heavy metals (lead, arsenic, cadmium, etc.); Biocalcium: Natural biocalcium currently mainly comes from oyster shells and shells, which are derived from lakes and offshore waters. Due to the pollution of oceans and lakes, there are reports that this type of calcium preparation is contaminated with elements such as lead and cadmium. Oyster shells often contain various heavy metal elements. Long-term use will cause these heavy metals to deposit in the body and cause chronic poisoning.

[0003] However, there have always been technical bottlenecks in the raw materials of safer calcium supplement products on the market, making it difficult to support consumer demand. Summary of the invention

[0004] The purpose of the present invention is to provide a calcium nutritional supplement composition and a bionic preparation method thereof, so as to solve the problem that there has always been a technical bottleneck in the raw material end of the existing safer calcium supplement products on the market, which is difficult to meet consumer demand.

[0005] To achieve the above object, the present invention provides a calcium nutritional supplement composition, wherein each component of the calcium nutritional supplement composition comprises, by percentage, 20-30% of lysine, 10-20% of calcium carbonate, 20-30% of whey protein, 20-30% of branched-chain amino acids, 10-20% of glutamine and 0.3-0.4% of mineral elements 0.3-0.4%, the branched-chain amino acids are one or more of leucine, valine and isoleucine, and the mineral elements are one or more of iron, zinc and magnesium.

[0006] The present invention also provides a bionic preparation method of a calcium nutritional supplement composition, which is used to prepare the calcium nutritional supplement composition as described above, comprising the following steps:

[0007] Adding the quantitative whey protein, lysine, branched-chain amino acids and glutamine into the reaction container in sequence;

[0008] Add 5 times the amount of digestive solution A to the reaction vessel, heat in a water bath, add 2% of the phospholipid in the formula, and stir to react for 3 hours;

[0009] Add 5 times the amount of digestive solution B to the reaction vessel, slightly adjust the pH to 2 with 0.1M HCl, heat in a water bath, add 0.5% grape seed polyphenols, and stir to react for 2 hours;

[0010] Add a certain amount of calcium carbonate and mineral elements to the reaction container, adjust the pH to 6, and stir and incubate for 1 hour;

[0011] The reaction container is concentrated under reduced pressure at 60° C. to a solid content of about 25%, and then dried to obtain the calcium nutritional supplement composition dry powder.

[0012] Among them, in the step of "adding 5 times the amount of digestive fluid A to the reaction container, heating in a water bath, adding 2% of the phospholipid in the formula, and reacting with stirring and keeping warm for 3 hours", the temperature to be reached for water bath heating is 37°C, and the temperature condition for the reaction with stirring and keeping warm is 37°C.

[0013] Among them, in the step of "add 5 times the amount of digestive solution B to the reaction container, slightly adjust the pH to 2 with 0.1M HCL, heat in a water bath, add 0.5% grape seed polyphenols in the formula, and stir the reaction for 2 hours", the temperature that needs to be reached for water bath heating is 37°C.

[0014] Among them, in the step of "adding 5 times the amount of digestive solution A to the reaction container, heating in a water bath, adding 2% of the phospholipid in the formula, and stirring and reacting for 3 hours at a temperature of 3 hours", the specific preparation method of digestive solution A is:

[0015] Potassium dihydrogen phosphate was dissolved in water to prepare a 1% solution, the pH value was adjusted to 7 with 0.1 mol / L sodium hydroxide solution, and 1% trypsin was added to prepare digestive solution A.

[0016] Among them, in the step of "adding 5 times the amount of digestive solution A to the reaction container, heating in a water bath, adding 2% of the lecithin in the formula, and stirring and reacting for 3 hours under heat preservation", the lecithin is in liquid state, and the preparation method of the lecithin liquid is specifically as follows:

[0017] Add 5 times the amount of 95% ethanol to soybean lecithin, dissolve, clarify and disperse by ultrasonication, add 2 times the amount of water, mix well, recover the ethanol under reduced pressure at 60°C, and obtain a semi-fluid aqueous extract of lecithin for use.

[0018] Among them, in the step of "adding 5 times the amount of digestive solution B to the reaction container, slightly adjusting the pH to 2 with 0.1M HCL, heating in a water bath, adding 0.5% grape seed polyphenols in the formula, and stirring the reaction for 2 hours", the specific preparation method of digestive solution B is: adding pepsin to a hydrochloric acid aqueous solution with a pH of 1.8 to prepare a 1% pepsin solution.

[0019] The present invention discloses a calcium nutritional supplement composition and a bionic preparation method thereof, wherein each component comprises, by percentage, 20-30% of lysine, 10-20% of calcium carbonate, 20-30% of whey protein, 20-30% of branched-chain amino acids, 10-20% of glutamine and 0.3-0.4% of mineral elements, and the materials adopted in the technical scheme are commonly used raw materials for dietary supplements, such as whey protein, branched-chain amino acids and calcium carbonate, which are frequently used in formulas such as milk powder and nutritional supplements, and are different from other formulas that require raw material sources that are difficult to be widely used to achieve the purpose of promoting calcium absorption. In addition, the material combination adopted in the technical scheme solves the application drawbacks and defects of the combined materials when used alone or in combination with other materials, converts low-cost inorganic calcium into organic calcium, significantly promotes the absorption and utilization of calcium, and also improves the sensory experience of eating, and the allergenicity of the overall formula is significantly reduced. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0021] Figure 1 The present invention is a flowchart of the steps of the bionic preparation method of the calcium nutritional supplement composition provided by the present invention.

[0022] Figure 2 It is a comparison chart of the cell viability test provided by the present invention.

[0023] Figure 3 It is a comparison chart of the calcium transport amount of Caco-2 monolayer cells provided by the present invention. DETAILED DESCRIPTION

[0024] Embodiments of the present invention are described in detail below, examples of which are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are intended to be used to explain the present invention, and should not be construed as limiting the present invention.

[0025] See also Figures 1 to 3 The present invention provides a calcium nutritional supplement composition, wherein each component of the calcium nutritional supplement composition comprises, by percentage, 20-30% of lysine, 10-20% of calcium carbonate, 20-30% of whey protein, 20-30% of branched-chain amino acids, 10-20% of glutamine and 0.3-0.4% of mineral elements, wherein the branched-chain amino acids are one or more of leucine, valine and isoleucine, and the mineral elements are one or more of iron, zinc and magnesium.

[0026] In this embodiment, the materials used in this technical solution are commonly used raw materials for dietary supplements, whey protein, branched-chain amino acids, calcium carbonate, which are frequently used in formulas such as milk powder and nutritional supplements. They are different from other formulations that require raw materials that are difficult to use widely to achieve the purpose of promoting calcium absorption. In addition, the material combination used in this technical solution solves the disadvantages of using the combined materials alone or in combination with other materials, converts low-cost inorganic calcium into organic calcium, significantly promotes the absorption and utilization of calcium, and also improves the sensory experience of eating it, and the overall allergenicity of the formula is significantly reduced.

[0027] See also Figure 1 The present invention also provides a bionic preparation method of a calcium nutritional supplement composition, which is used to prepare the calcium nutritional supplement composition as described above, comprising the following steps:

[0028] S1: adding the quantified whey protein, lysine, branched-chain amino acids and glutamine into a reaction container in sequence;

[0029] S2: Add 5 times the amount of digestive solution A to the reaction vessel, heat it in a water bath to 37°C, add 2% of the phospholipid in the formula, and stir and react at 37°C for 3 hours;

[0030] S3: Add 5 times the amount of digestive solution B to the reaction vessel, slightly adjust the pH to 2 with 0.1M HCl, heat in a water bath to 37°C, add 0.5% grape seed polyphenols, and stir to react for 2 hours;

[0031] S4: adding a certain amount of calcium carbonate and mineral elements to the reaction container, adjusting the pH to 6, and incubating with stirring for 1 hour;

[0032] S5: The reaction container is concentrated under reduced pressure at 60° C. to a solid content of about 25%, and then dried to obtain the calcium nutritional supplement composition dry powder.

[0033] Furthermore, the specific preparation method of digestive solution A is as follows: potassium dihydrogen phosphate is added into water to dissolve it to prepare a 1% solution, the pH value is adjusted to 7 with 0.1 mol / L sodium hydroxide solution, and 1% trypsin is added to prepare digestive solution A.

[0034] Furthermore, the phospholipid is in liquid state, and the preparation method of the phospholipid liquid is specifically as follows: add 5 times the amount of 95 ethanol to soybean lecithin, dissolve and clarify it by ultrasonic dissolution, add 2 times the amount of water, mix well, and recover the ethanol under reduced pressure at 60° C. to obtain a semi-fluid aqueous extract of phospholipid for use.

[0035] Furthermore, the specific preparation method of digestive solution B is: adding pepsin to a hydrochloric acid aqueous solution with a pH of 1.8 to prepare a 1% pepsin solution.

[0036] In this embodiment, calcium carbonate has low cost and high calcium content, but it is criticized for consuming gastric acid during use, causing bloating and constipation, and having low calcium absorption and utilization rate. Whey protein is well absorbed and utilized, and is an important protein source for product formulations such as nutritional supplements, but it is easy to cause allergies in some groups. Therefore, the present technical solution is processed by a preparation method of bionic gastrointestinal digestion simulation to simulate the natural digestive environment of the human body, which is closer to the digestion process after human ingestion. The conditions are mild and safe, and there are fewer uncertain risk factors introduced. The absorption performance and defects of the formula materials are significantly improved. In addition, the present technical solution does not require complex separation and purification steps, and industrial production is more feasible and commercially valuable. At the same time, the present technical solution can enable the materials to cooperate with each other to achieve high bioavailability absorption and low allergenicity, and the sensory experience is also improved.

[0037] Embodiment 1:

[0038] The present invention also provides a bionic preparation method of a calcium nutritional supplement composition, comprising the following steps:

[0039] 25g whey protein, 25g lysine, 8g leucine, 8g valine, 8g isoleucine, 15g glutamine were added to the reaction container in sequence, 500g digestive solution A was added, heated to 37°C in a water bath, 2g phospholipids (based on dry matter) were added, and the mixture was stirred and reacted at 37°C for 3h, 500g digestive solution B was added, the pH was slightly adjusted to 2 with 0.1M HCL, heated to 37°C in a water bath, 0.5g grape seed polyphenol was added, and the mixture was stirred and reacted for 2h, 15g calcium carbonate, 0.6g ferrous gluconate, 1.6g zinc gluconate, and 2g magnesium citrate were added, the pH was adjusted to 6, and the mixture was stirred and incubated for 1h. The mixture was concentrated under reduced pressure at 60°C to a solid content of about 25%, and spray dried to obtain dry powder.

[0040] Embodiment 2:

[0041] The present invention also provides a bionic preparation method of a calcium nutritional supplement composition, comprising the following steps:

[0042] 25g whey protein, 25g lysine, 8g leucine, 8g valine, 8g isoleucine, 15g glutamine were added to the reaction container in sequence, 800g digestive solution A was added, heated to 37°C in a water bath, 1g phospholipid (based on dry matter) was added, and the mixture was stirred and reacted at 37°C for 3h, 500g digestive solution B was added, the pH was slightly adjusted to 2 with 0.1M HCL, heated to 37°C in a water bath, 0.5g grape seed polyphenol was added, and the mixture was stirred and reacted for 2h, 15g calcium carbonate, 0.6g ferrous gluconate, 1.6g zinc gluconate, and 2g magnesium citrate were added, the pH was adjusted to 6, and the mixture was stirred and incubated for 1h. The mixture was concentrated under reduced pressure at 60°C to a solid content of about 40%, and freeze-dried to obtain a dry powder.

[0043] Embodiment 3:

[0044] The present invention also provides a bionic preparation method of a calcium nutritional supplement composition, comprising the following steps:

[0045] 30g whey protein, 15g lysine, 8g leucine, 8g valine, 8g isoleucine, 25g glutamine were added to the reaction container in sequence, 500g digestive solution A was added, heated to 37°C in a water bath, 2g phospholipids (based on dry matter) were added, and the mixture was stirred and reacted at 37°C for 3h, 500g digestive solution B was added, the pH was slightly adjusted to 2 with 0.1M HCL, heated to 37°C in a water bath, 0.5g grape seed polyphenol was added, and the mixture was stirred and reacted for 2h, 10g calcium carbonate, 0.6g ferrous gluconate, 1.6g zinc gluconate, and 2g magnesium citrate were added, the pH was adjusted to 6, and the mixture was stirred and incubated for 1h. The mixture was concentrated under reduced pressure at 60°C to a solid content of about 25%, and spray dried to obtain dry powder.

[0046] Comparative Example 1 (Formula Adjusted Protein) 25g of lysine, 13g of leucine, 13g of valine, 13g of isoleucine, and 25g of glutamine were added to the reaction container in sequence, 500g of digestive solution A was added, heated to 37°C in a water bath, 2g of phospholipids (based on dry matter) were added, and the mixture was stirred and reacted at 37°C for 3h, 500g of digestive solution B was added, the pH was slightly adjusted to 2 with 0.1M HCL, heated to 37°C in a water bath, 0.5g of grape seed polyphenol was added, and the mixture was stirred and reacted for 2h, 15g of calcium carbonate, 0.6g of ferrous gluconate, 1.6g of zinc gluconate, and 2g of magnesium citrate were added, the pH was adjusted to 6, and the mixture was stirred and incubated for 1h. The mixture was concentrated under reduced pressure at 60°C to a solid content of about 25%, and spray-dried to obtain a dry powder.

[0047] Comparative Example 2 (Formula Adjustment of Amino Acids) 55g of whey protein, 15g of lysine, 3g of leucine, 3g of valine, 8g of isoleucine, and 5g of glutamine were added to the reaction container in sequence, 500g of digestive solution A was added, heated to 37°C in a water bath, 2g of phospholipids (based on dry matter) were added, and the mixture was stirred and reacted at 37°C for 3h, 500g of digestive solution B was added, the pH was slightly adjusted to 2 with 0.1M HCL, heated to 37°C in a water bath, 0.5g of grape seed polyphenols was added, and the mixture was stirred and reacted for 2h, 15g of calcium carbonate, 0.6g of ferrous gluconate, 1.6g of zinc gluconate, and 2g of magnesium citrate were added, the pH was adjusted to 6, and the mixture was stirred and incubated for 1h. The mixture was concentrated under reduced pressure at 60°C to a solid content of about 25%, and spray-dried to obtain a dry powder.

[0048] Comparative Example 3 (Digestion Process Adjustment, Phospholipids) 25g of whey protein, 25g of lysine, 8g of leucine, 8g of valine, 8g of isoleucine, and 15g of glutamine were added to the reaction container in sequence, 500g of digestion solution A was added, the mixture was heated to 37°C in a water bath, and stirred and reacted at 37°C for 3h, 500g of digestion solution B was added, the pH was slightly adjusted to 2 with 0.1M HCL, the mixture was heated to 37°C in a water bath, 0.5g of grape seed polyphenol was added, and the mixture was stirred and reacted for 2h, 15g of calcium carbonate, 0.6g of ferrous gluconate, 1.6g of zinc gluconate, and 2g of magnesium citrate were added, the pH was adjusted to 6, and the mixture was stirred and incubated for 1h. The mixture was concentrated under reduced pressure at 60°C to a solid content of about 25%, and spray-dried to obtain a dry powder.

[0049] Comparative Example 4 (Digestion Process Adjustment, Grape Seed Polyphenols) 25g of whey protein, 25g of lysine, 8g of leucine, 8g of valine, 8g of isoleucine, and 15g of glutamine were added to a reaction container in sequence, 500g of digestion solution A was added, heated to 37°C in a water bath, 2g of phospholipids (based on dry matter) were added, and the mixture was stirred and reacted at 37°C for 3h, 500g of digestion solution B was added, the pH was slightly adjusted to 2 with 0.1M HCL, heated to 37°C in a water bath, stirred and reacted for 2h, 15g of calcium carbonate, 0.6g of ferrous gluconate, 1.6g of zinc gluconate, and 2g of magnesium citrate were added, the pH was adjusted to 6, and stirred and incubated for 1h. The mixture was concentrated under reduced pressure at 60°C to a solid content of about 25%, and spray-dried to obtain a dry powder.

[0050] The present invention also provides the determination and results of calcium binding capacity of the composition:

[0051] Calcium binding rate determination method:

[0052] There are many different methods for testing the binding capacity of amino acids, peptides and proteins to calcium ions, and the testing methods vary depending on the material. For example, patent CN 106855785 A uses o-cresolphthalein colorimetric method to test the chelating capacity of schizont meal chelating peptide to calcium, and the calcium chelating activity is 88ug / mg, and the chelating activity of the purified specific peptide SPH-1A is 176.34ug / mg; patent CN 111528480A uses gel chromatography separation method to determine the calcium holding capacity of silk raw silk collagen peptide to be 0.0136g / g; patent CN112094881 A uses centrifugal separation method to determine the chelating activity of black bean protein peptide to be 77.54ug / mg, and after separation and purification, the chelating activity of pure protein peptide is 101.45ug / mg; other documents also report different binding amounts. The calcium chelating capacity is determined by comparing with the o-cresolphthalein colorimetric method of patent CN 106855785 A.

[0053] The results show that the digestion product obtained by the patented method has a calcium binding capacity of about 120-160ug / mg, which improves the binding capacity of the material and calcium compared with existing similar technologies.

[0054] Table 1: Summary of calcium chelating capacity and sensory evaluation of samples in various examples

[0055]

[0056]

[0057] Cytotoxicity test of different samples: The concentration of each sample was adjusted, and the MTT method was used to determine the effect of each sample concentration on the activity of Caco-2 cells.

[0058] Calcium transport and absorption test:

[0059] Calcium transport test of different samples: Test: prepare sample solutions of different concentrations, dissolve in buffer solution HBSS, and heat in a water bath at 37°C. Take the established monolayer model, wash the upper and lower chambers of the 12-well TransWellTM plate, add 1.5mL preheated HBSS (calcium-free) to the lower chamber, add 0.5mL CaCl2 solution to the upper chamber, take samples of the lower chamber solution in the 0-3h period, measure their calcium content and calculate the calcium transport amount. Combined calcium sample transport test: The operation is the same as the calcium ion transport test. After washing, add 1.5mL preheated HBSS to the lower chamber, add 0.5mL of the combined calcium sample to the upper chamber, take the lower chamber solution in 0-3h, and measure its calcium content.

[0060] The results showed that the calcium turnover of the samples after digestion was significantly increased and promoted.

[0061] Advantage 3 Composition Improves Sensory Experience:

[0062] Through a combination of online and offline methods, questionnaires were distributed to the trial group. The questionnaire content included symptoms before the trial and the improvement of symptoms after the trial: the trial sample size was 30 people, covering different ages and genders. Trial dosage: 500mg / capsule, two capsules a day, trial time: 1 week.

[0063] Table 2 Basic information of the food test population

[0064]

[0065] Table 3 Statistical results of online and offline food tasting questionnaires

[0066]

[0067]

[0068] Compared with the initial untreated sample, after digestion, the flatulence, burning sensation, constipation and the like of the group taking the combination were significantly improved.

[0069] In summary, the materials used in this technical solution are commonly used raw materials for dietary supplements, whey protein, branched-chain amino acids, calcium carbonate, which are frequently used in formulas such as milk powder and nutritional supplements. They are different from other formulations that require raw materials that are difficult to widely use to achieve the purpose of promoting calcium absorption.

[0070] The preparation method adopted in this technical solution simulates the natural digestive environment of the human body, which is closer to the digestive process after ingestion by the human body. The conditions are mild and safe, and there are few uncertain risk factors. It significantly solves and improves the absorption performance and defects of the formula materials. It does not require complicated separation and purification steps, and the industrial production is more feasible and has more commercial value.

[0071] The material combination and preparation method adopted in this technical solution solve the application drawbacks of using the combined material alone or in combination with other materials. The low-cost inorganic calcium is converted into organic calcium, which significantly promotes the absorption and utilization of calcium, and also improves the sensory experience of eating it, and the allergenicity of the overall formula is significantly reduced.

[0072] Calcium carbonate is low-cost and high in calcium, but it is criticized for consuming gastric acid during use, causing bloating and constipation, and having a low calcium absorption rate. Whey protein is well absorbed and utilized, and is an important protein source for product formulations such as nutritional supplements, but it is prone to cause allergies in some groups. After being processed by a preparation method that simulates bionic gastrointestinal digestion, the performance of the composition can be significantly improved, and the materials work together to achieve high bioavailability absorption and low allergenicity, and the sensory experience is also improved.

[0073] What is disclosed above is only a preferred embodiment of the present invention, and it certainly cannot be used to limit the scope of rights of the present invention. Ordinary technicians in this field can understand that all or part of the processes of the above embodiment and equivalent changes made according to the claims of the present invention still fall within the scope of the invention.

Claims

1. A calcium nutritional supplement composition, characterized in that: Each component includes, by percentage, 20-30% lysine, 10-20% calcium carbonate, 20-30% whey protein, 20-30% branched-chain amino acids, 10-20% glutamine and 0.3-0.4% mineral elements, wherein the branched-chain amino acids are one or more of leucine, valine and isoleucine, and the mineral elements are one or more of iron, zinc and magnesium.

2. A bionic preparation method of a calcium nutritional supplement composition, for preparing the calcium nutritional supplement composition as claimed in claim 1, characterized in that: The steps include: Adding the quantitative whey protein, lysine, branched-chain amino acids and glutamine into the reaction container in sequence; Add 5 times the amount of digestive solution A to the reaction vessel, heat in a water bath, add 2% of the phospholipid in the formula, and stir to react for 3 hours; Add 5 times the amount of digestive solution B to the reaction vessel, slightly adjust the pH to 2 with 0.1M HCl, heat in a water bath, add 0.5% grape seed polyphenols, and stir to react for 2 hours; Add a certain amount of calcium carbonate and mineral elements to the reaction container, adjust the pH to 6, and stir and incubate for 1 hour; The reaction container is concentrated under reduced pressure at 60° C. to a solid content of about 25%, and then dried to obtain the calcium nutritional supplement composition dry powder.

3. The bionic preparation method of the calcium nutritional supplement composition according to claim 2, characterized in that: In the step "add 5 times the amount of digestive fluid A to the reaction container, heat in a water bath, add 2% of the phospholipid in the formula, and react with stirring and insulation for 3 hours", the temperature to be reached for water bath heating is 37°C, and the temperature condition for the insulation and stirring reaction is 37°C.

4. The bionic preparation method of the calcium nutritional supplement composition according to claim 3, characterized in that: In the step "add 5 times the amount of digestive solution B to the reaction vessel, slightly adjust the pH to 2 with 0.1 M HCL, heat in a water bath, add 0.5% grape seed polyphenols in the formula, and stir to react for 2 hours", the temperature to be reached by heating in the water bath is 37°C.

5. The biomimetic preparation method of the calcium nutritional supplement composition according to claim 4, characterized in that: In the step "adding 5 times the amount of digestive solution A to the reaction vessel, heating in a water bath, adding 2% of the phospholipid in the formula, and stirring and reacting for 3 hours at a temperature-insulated state", the specific preparation method of digestive solution A is: Potassium dihydrogen phosphate was dissolved in water to prepare a 1% solution, the pH value was adjusted to 7 with 0.1 mol / L sodium hydroxide solution, and 1% trypsin was added to prepare digestive solution A.

6. The biomimetic preparation method of the calcium nutritional supplement composition according to claim 5, characterized in that: In the step of "adding 5 times the amount of digestive solution A to the reaction container, heating in a water bath, adding 2% of the lecithin in the formula, and stirring and reacting for 3 hours under heat preservation", the lecithin is in liquid state, and the preparation method of the lecithin liquid is specifically as follows: Add 5 times the amount of 95% ethanol to soybean lecithin, dissolve, clarify and disperse by ultrasonication, add 2 times the amount of water, mix well, recover the ethanol under reduced pressure at 60°C, and obtain a semi-fluid aqueous extract of lecithin for use.

7. The calcium nutritional supplement composition and the biomimetic preparation method thereof according to claim 6, characterized in that: In the step "add 5 times the amount of digestive solution B as the component to the reaction container, slightly adjust the pH to 2 with 0.1M HCL, heat in a water bath, add 0.5% grape seed polyphenol as the component, and stir to react for 2 hours", the specific preparation method of digestive solution B is: add pepsin to a hydrochloric acid aqueous solution with a pH of 1.8 to prepare a 1% pepsin solution.

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