Fungicide for treating benzalkonium chloride-containing wastewater and application thereof

Abstract

The invention belongs to the technical field of wastewater treatment, and particularly relates to a fungicide for treating wastewater containing benzalkonium chloride and application of the fungicide. Wherein the mass concentration percentage of each strain is as follows: 32.37% of pseudomonas, 21.94% of rhizobium, 14.69% of achromobacter, 4.65% of stenotrophomonas, 3.79% of tisteuria mobilis, 2.41% of flavobacterium, 2.26% of alcaligenes, 1.88% of acetogenic proteophilic bacteria, 1.59% of bacillus and 0.53% of balloonium, and the degradation rate of COD (Chemical Oxygen Demand) in the benzalkonium chloride-containing wastewater can be improved.

Description

Technical Field

[0001] The invention belongs to the technical field of wastewater treatment, and particularly relates to a bacterial agent for treating wastewater containing benzalkonium chloride and application thereof. Background Art

[0002] In recent years, people have become more stringent in their requirements for personal hygiene and living environment. The popularity of medicines and personal care products has become an indispensable daily necessity for people, such as human or veterinary medicines including anti-inflammatory drugs, analgesics and antibiotics, as well as disinfectants or fragrances used for personal care. The wastewater discharged by enterprises producing the above-mentioned daily necessities is often rich in benzalkonium chloride. Benzalkonium chloride is the most common active ingredient in disinfectants. It has a broad spectrum and high efficiency in killing bacteria and algae, and can effectively control the growth of bacteria, algae and slime in water bodies. This type of disinfection wastewater not only contains suspended matter, but also contains some disinfectant residues that are difficult to biodegrade. This wastewater inevitably harms the water body and the aquatic ecological environment.

[0003] Compared with physical and chemical methods, biological methods for treating wastewater containing benzalkonium chloride, such as mixed bed aerated biological filter, have the advantages of low cost, simple operation and no secondary pollution. However, biological methods have the problems of low treatment efficiency, greater environmental influences and unstable treatment effects. To address the above problems, the key is to maximize the performance of microorganisms and use their metabolism to degrade organic pollutants in wastewater. Studies have shown that although the introduction of a single strain of bacteria has a certain treatment effect, there are still problems such as long reaction time and unstable treatment effect.

[0004] Therefore, in order to improve the efficiency of wastewater biological treatment and achieve stable operation, it is crucial to obtain a composite bacterial agent with high efficiency in degrading organic pollutants. Summary of the invention

[0005] In order to solve the problems of the background technology, the present invention provides a bacterial agent for treating wastewater containing benzalkonium chloride and application thereof.

[0006] The technical solution of the present invention is as follows:

[0007] The present invention provides a bacterial agent for treating wastewater containing benzalkonium chloride, comprising the following components:

[0008] Pseudomonas, Achromobacter, Rhizobium, Stenotrophomonas, Tistrella motilium, Flavobacterium, Alcaligenes, Proteophilus acetogenus, Bacillus and Aeromonas.

[0009] The mass percentage of each component is 32.37%-43.7% for Pseudomonas, 14.69%-44.33% for Achromobacter, 1.2%-28.53% for Rhizobium, 4.65%-7.81% for Stenotrophomonas, 0.02%-3.79% for Tistrina mobilis, 2.21%-2.41% for Flavobacterium, 0.01%-2.26% for Alcaligenes, 0.19%-1.88% for Acetaminophen, 0.22%-1.59% for Bacillus and 0.53%-0.83% for Aeromonas.

[0010] Preferably, the mass percentages of Pseudomonas, Achromobacter, Stenotrophomonas and Aeromonas are 32.37%, 14.69%, 4.65% and 0.53% respectively, and the mass percentages of Rhizobium, Tistrina mobilis, Flavobacterium, Alcaligenes, Acetogenic Proteophilus and Bacillus are 21.94%, 3.79%, 2.41%, 2.26%, 1.88% and 1.59% respectively.

[0011] The present invention also provides an application of the bacterial agent for treating wastewater containing benzalkonium chloride, comprising: adding 5 g / L or 10 g / L of the bacterial agent to the wastewater containing benzalkonium chloride to be treated, performing anaerobically treatment at 28-34° C. for 5 days, and then performing aerobic treatment.

[0012] The method also includes: setting the temperature during the treatment process, using untreated benzalkonium chloride-containing wastewater to replace the treated benzalkonium chloride-containing wastewater mixture or its filtered supernatant, or adding nutrient salts or adsorption carriers during the treatment process.

[0013] During the treatment process, the temperature was set at 32°C, and the untreated benzalkonium chloride-containing wastewater was used to replace the supernatant of the treated benzalkonium chloride-containing wastewater mixture every day, and 0.25-0.5 g / L of potassium dihydrogen phosphate was added every three days.

[0014] During the treatment process, the temperature was set at 34°C, and the untreated benzalkonium chloride-containing wastewater was used to replace the supernatant of the treated benzalkonium chloride-containing wastewater mixture every day, and 0.25-0.5 g / L of potassium dihydrogen phosphate was added every three days.

[0015] During the treatment process, the temperature was set at 34°C, and the supernatant of the treated benzalkonium chloride-containing wastewater mixture was replaced with untreated benzalkonium chloride-containing wastewater every three days, and 0.25-0.5 g / L of potassium dihydrogen phosphate was added every three days.

[0016] During the treatment process, the temperature was set at 34°C, and the treated benzalkonium chloride-containing wastewater mixture was replaced with untreated benzalkonium chloride-containing wastewater every three days. 0.5 g / L of potassium dihydrogen phosphate and 5 g / L of activated carbon were added every three days.

[0017] The COD removal rate reached 93.51%.

[0018] Beneficial Effects

[0019] The invention provides a bacterial agent for treating wastewater containing benzalkonium chloride. The degradation efficiency of the substrate is enhanced by regulating experimental environmental parameters, and the operation is simple and the pertinence is strong. The prepared composite bacterial agent has high removal efficiency for pollutants and can realize stable operation of wastewater.

[0020] The main purpose of the bacterial agent provided by the present invention is to deal with the COD degradation problem in disinfection wastewater containing benzalkonium chloride, maintain stable water quality operation, integrate dominant bacteria in the wastewater, and increase the degradation rate of COD in such wastewater, thereby realizing green discharge of wastewater. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 This is the change diagram of COD concentration in wastewater under different dosage of bacterial agent in Example 1;

[0022] Figure 2 This is the change chart of COD concentration and removal rate of inlet and outlet water under different parameter conditions of adding bacterial agent in Example 2;

[0023] Figure 3 Schematic diagram of the percentage of bacterial species composition at the genus level in each system under the optimal parameter conditions of Example 3. DETAILED DESCRIPTION

[0024] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings in the embodiments of the present invention.

[0025] A bacterial agent for treating wastewater containing benzalkonium chloride, comprising the following components:

[0026] Pseudomonas (microorganisms of the genus Pseudomonas), Achromobacter (microorganisms of the genus Achromobacter), Rhizobium (microorganisms of the genus Rhizobiaceae), Stenotrophomonas (microorganisms of the genus Stenotrophomonas), Tistrella (microorganisms of the genus Tistrella), Flavobacterium (microorganisms of the genus Flavobacterium), Alcaligens (microorganisms of the genus Alcaligenaceae), Proteiniphilum (microorganisms of the genus Proteiniphilum), Bacillus (microorganisms of the genus Bacillus) and Aerococcus (microorganisms of the genus Aerococcus).

[0027] The mass percentage of each component is 32.37%-43.7% for Pseudomonas, 14.69%-44.33% for Achromobacter, 1.2%-28.53% for Rhizobium, 4.65%-7.81% for Stenotrophomonas, 0.02%-3.79% for Tistrina mobilis, 2.21%-2.41% for Flavobacterium, 0.01%-2.26% for Alcaligenes, 0.19%-1.88% for Acetaminophen, 0.22%-1.59% for Bacillus and 0.53%-0.83% for Aeromonas.

[0028] Preferably, the mass percentages of Pseudomonas, Achromobacter, Oligotrophomonas and Aeromonas are 32.37%, 14.69%, 4.65% and 0.53% respectively, and the mass percentages of Rhizobium, Tistrina mobilis, Flavobacterium, Alcaligenes, Acetogenic Proteinophilic Bacteria and Bacillus are 21.94%, 3.79%, 2.41%, 2.26%, 1.88% and 1.59% respectively. The bacterial agent is used to treat wastewater containing benzalkonium chloride and has the highest COD removal rate, which is as high as 93.51%.

[0029] The present invention also provides an application of the bacterial agent for treating wastewater containing benzalkonium chloride, comprising: adding 5 g / L or 10 g / L of the bacterial agent to the wastewater containing benzalkonium chloride to be treated, performing anaerobically treatment at 28-34° C. for 5 days, and then performing aerobic treatment.

[0030] The method also includes: setting the temperature during the treatment process, using untreated benzalkonium chloride-containing wastewater to replace the treated benzalkonium chloride-containing wastewater mixture or its filtered supernatant, or adding nutrient salts or adsorption carriers during the treatment process.

[0031] During the treatment process, the temperature was set at 32°C, and the untreated benzalkonium chloride-containing wastewater was used to replace the supernatant of the treated benzalkonium chloride-containing wastewater mixture every day, and 0.25-0.5 g / L of potassium dihydrogen phosphate was added every three days.

[0032] During the treatment process, the temperature was set at 34°C, and the untreated benzalkonium chloride-containing wastewater was used to replace the supernatant of the treated benzalkonium chloride-containing wastewater mixture every day, and 0.25-0.5 g / L of potassium dihydrogen phosphate was added every three days.

[0033] During the treatment process, the temperature was set at 34°C, and the supernatant of the treated benzalkonium chloride-containing wastewater mixture was replaced with untreated benzalkonium chloride-containing wastewater every three days, and 0.25-0.5 g / L of potassium dihydrogen phosphate was added every three days.

[0034] During the treatment process, the temperature was set at 34°C, and the treated benzalkonium chloride-containing wastewater mixture was replaced with untreated benzalkonium chloride-containing wastewater every three days. 0.5 g / L of potassium dihydrogen phosphate and 5 g / L of activated carbon were added every three days.

[0035] The invention enhances the degradation efficiency of the substrate by regulating the experimental environment parameters, has simple operation and strong pertinence, and the prepared bacterial agent has high removal efficiency of pollutants in benzalkonium chloride wastewater and can achieve stable operation of water quality.

[0036] The main purpose of the bacterial agent of the present invention is to deal with the COD degradation problem in disinfection wastewater containing benzalkonium chloride, maintain stable water quality operation, integrate superior bacterial species, and increase the degradation rate of COD in such wastewater, thereby realizing green discharge of wastewater.

[0037] Example 1

[0038] The wastewater in this example was taken from the benzalkonium chloride disinfection wastewater of a daily necessities company in Suzhou, and its COD concentration was 2964 mg / L. Two parallel control groups D1, D2 and D3, D4 were set up, and 200 mL of wastewater was taken from each control group. The dosage of bacterial agents in D1 and D2 was 5 g / L, and the dosage of bacterial agents in D3 and D4 was 10 g / L. From June 28 to July 12, 2024, the system did not change water during this operation period, the set temperature was 32 ° C, and 0.1 g of potassium dihydrogen phosphate was added every three days to explore the effect of different dosages of bacterial agents on the COD degradation effect in wastewater.

[0039] The experimental results show that the COD concentration has dropped rapidly since the system started anaerobic operation for 5 days since June 28. On July 3, the COD concentrations of D1, D2, D3 and D4 dropped to 2498 mg / L, 2574 mg / L, 2242 mg / L and 1881 mg / L, respectively, and the degradation rates of D3 and D4 were significantly greater than those of D1 and D2. After switching to aerobic operation on July 5, COD continued to be degraded. On July 6, the COD concentrations of D1 and D2 dropped to 2358 mg / L and 2016 mg / L, respectively, while those of D3 and D4 dropped to 1790 mg / L and 1246 mg / L, respectively. After a week of aerobic operation without water replacement, the COD of the wastewater containing benzalkonium chloride continued to decrease, and the COD concentrations of D1, D2, D3, and D4 were stabilized at 1520 mg / L, 1760 mg / L, 812.7 mg / L, and 857.8 mg / L, respectively. The COD removal rates of D1, D2, D3, and D4 were 48.72%, 40.62%, 72.58%, and 71.06%, respectively.

[0040] The comparison shows that the COD removal efficiency of 10g / L bacterial agent dosage in wastewater containing benzalkonium chloride is better than that of 5g / L bacterial agent dosage.

[0041] Example 2

[0042] The wastewater in this example was also taken from the benzalkonium chloride disinfection wastewater of a daily necessities company in Suzhou, and its COD concentration was 2964 mg / L. The original wastewater concentration L0 was set, and two parallel control groups L1, L2 and L3, L4 were used. 200 mL of wastewater was taken from each control group, among which L1 and L2 were the treatment conditions of D1 and D2 in stable operation of Example 1, and L3 and L4 were the treatment conditions of D3 and D4 in stable operation of Example 1. The COD removal rates of L1, L2, L3, and L4 were Q1, Q2, Q3, and Q4, respectively. From July 12 to November 6, the degradation capacity of COD in the wastewater was further improved by changing parameters including temperature, water change frequency, water change type, nutrient salt and adsorption carrier addition.

[0043] Among them, the temperature is 32℃ or 34℃, the water change frequency is every day / time or every three days / time, the water change type is supernatant (replacing 200mL of filtered supernatant with original wastewater) or muddy water mixture (replacing 200mL of unfiltered mixture with original wastewater), and the nutrient salt and adsorption carrier are potassium dihydrogen phosphate and activated carbon respectively.

[0044] Since July 12, the frequency of water change for L1, L2, L3, and L4 has been every day / time, and the water change type is all supernatant. 0.1g potassium dihydrogen phosphate is added every three days / time, and no adsorption carrier is added. When the temperature rises from 32°C to 34°C (July 26), the degradation rate of COD increases significantly. The COD concentrations of L1 and L2 dropped from 1896mg / L and 1711mg / L to 1384mg / L and 1038mg / L, respectively. Q1 and Q2 increased by 17.28% and 22.71%, respectively. The COD concentrations of L3 and L4 showed a continuous downward trend after the temperature was raised. With the increase in temperature, the cell metabolism of microorganisms is accelerated, the growth of microorganisms is promoted, which is beneficial to the COD degradation of the wastewater and improves the removal efficiency of COD.

[0045] At a temperature of 34°C, the water change types of L1, L2, L3, and L4 were all supernatant, and 0.1g potassium dihydrogen phosphate was added every three days. Without adding adsorption carriers, the water change frequency was changed during the operation from July 26 to August 27. L1 and L3 were changed every day, and L2 and L4 were changed every three days. During this test phase, the COD concentrations of L1, L2, L3, and L4 fluctuated up and down, and the degradation rate slowed down. The COD concentrations eventually reached 1023mg / L, 797.6mg / L, 993.3mg / L, and 737.4mg / L, respectively. Q1, Q2, Q3, and Q4 were 65.49%, 73.09%, 66.49%, and 75.12%, respectively. The COD removal rates of L2 and L4 were significantly higher than those of L1 and L3. It can be seen that extending the frequency of water changes and increasing the reaction time have a certain effect on the COD degradation of microorganisms using organic matter in wastewater.

[0046] Based on the results of the previous tests, during the operation period from August 27 to September 29, the temperature was maintained at 34°C, and the water change frequency of L1, L2, L3, and L4 was changed to every three days / time, and 0.1g of potassium dihydrogen phosphate was added every three days / time. The water change type was changed, L1 and L3 were replaced with mud and water mixture, and L2 and L4 were replaced with supernatant. At the same time, on August 31, 1g of activated carbon was added to L1 and L4, and L2 and L3 were not added. With the addition of carriers, the sludge flocs were gradually adsorbed by activated carbon, and the amount of microorganisms increased. On August 31, the COD concentrations of L1 and L4 dropped significantly, from 1023mg / L and 737.4mg / L to 707.3mg / L and 526.7mg / L, respectively. As the test continued, the COD concentrations of L1 and L4 eventually dropped to 310.8mg / L and 391.8mg / L, respectively, while those of Q1 and Q4 were as high as 89.51% and 86.78%, respectively. After achieving quantitative sludge discharge, the COD concentration in the wastewater was continuously degraded, and the COD concentration of L2 was reduced to 594.5mg / L, and the COD concentration of L3 was reduced to 418.9mg / L. Q2 and Q3 were as high as 79.94% and 85.87%, respectively. Compared with L2, the COD degradation effect of L3 is slightly better. The operation results show that under the dual effects of adding adsorption carriers and sludge discharge, the COD degradation efficiency of the wastewater is higher.

[0047] From October 8 to November 6, L1, L2, L3, and L4 were combined with the above-mentioned optimal parameter conditions, that is, the temperature was 34°C, the water change frequency was every three days / time, the water change type was muddy water mixture, and potassium dihydrogen phosphate was supplemented with 0.5g / L every three days / time, and activated carbon was added with 5g / L. During this period, L0 was increased to 5500mg / L. Under the condition of high load organic matter concentration, the system of this embodiment can still operate stably, and the COD removal rate is maintained at a high level. Q1, Q2, Q3, and Q4 reached 92%, 91.94%, 91.65%, and 93.51%, respectively.

[0048] The above test results show that the system L4, which is operated under the optimal parameter conditions with the addition of 10g / L bacterial agent, has the best removal effect on COD in wastewater containing benzalkonium chloride.

[0049] Example 3

[0050] Depend on Figure 3 It can be seen that the bacterial species composition analysis of each system L1, L2, L3, and L4 under the optimal parameter conditions corresponds to Figure 3 Among T1, T2, T3, and T4, it was found that the components of the bacterial agents with high COD degradation ability include Pseudomonas (microorganisms of the genus Pseudomonas), Achromobacter (microorganisms of the genus Achromobacter), Rhizobium (microorganisms of the genus Rhizobiaceae), Stenotrophomonas (microorganisms of the genus Stenotrophomonas), Tistrella (microorganisms of the genus Tistrella), Flavobacterium (microorganisms of the genus Flavobacterium), Alcaligenaceae (microorganisms of the genus Alcaligenaceae), Proteiniphilum (microorganisms of the genus Proteiniphilum), Bacillus (microorganisms of the genus Bacillus) and Aerococcus (microorganisms of the genus Aerococcus).

[0051] Measured on the basis of pure bacteria, the mass percentage of bacterial cells in each component is 32.37%-43.7% for Pseudomonas, 14.69%-44.33% for Achromobacter, 1.2%-28.53% for Rhizobium, 4.65%-7.81% for Stenotrophomonas, 0.02%-3.79% for Tistrina mobilis, 2.21%-2.41% for Flavobacterium, 0.01%-2.26% for Alcaligenes, 0.19%-1.88% for acetic acid-producing protein bacteria, 0.22%-1.59% for Bacillus and 0.53%-0.83% for Aeromonas.

[0052] Under the optimal parameter conditions, i.e., adding 10g / L bacterial agent, temperature of 34℃, water change frequency of every three days, water change type of mud water mixture, supplementing potassium dihydrogen phosphate 0.5g / L and adding activated carbon 5g / L every three days, compared with T1, T2, and T3 systems, the mass percentage of Achromobacter, Pseudomonas, Oligotrophomonas, and Aeromonas in T4 system was the lowest, which were 14.69%, 32.37%, 4.65%, and 0.53%, respectively. The mass percentage of Rhizobium, Tistrina motilium, Flavobacterium, Alcaligenes, Acetogenic Proteinophilic Bacteria, and Bacillus was the highest in T4 system, which were 21.94%, 3.79%, 2.41%, 2.26%, 1.88%, and 1.59%, respectively. The synergistic effect of microorganisms promoted the efficient degradation of high COD concentration in wastewater containing benzalkonium chloride, and achieved stable operation of the system.

Claims

1. A bacterial agent for treating wastewater containing benzalkonium chloride, characterized in that: Includes the following components: Pseudomonas, Achromobacter, Rhizobium, Stenotrophomonas, Tistrella motilium, Flavobacterium, Alcaligenes, Proteophilus acetogenus, Bacillus and Aeromonas.

2. The bacterial agent for treating wastewater containing benzalkonium chloride according to claim 1, characterized in that: The mass percentage of each component is 32.37%-43.7% for Pseudomonas, 14.69%-44.33% for Achromobacter, 1.2%-28.53% for Rhizobium, 4.65%-7.81% for Stenotrophomonas, 0.02%-3.79% for Tistrina mobilis, 2.21%-2.41% for Flavobacterium, 0.01%-2.26% for Alcaligenes, 0.19%-1.88% for Acetaminophen, 0.22%-1.59% for Bacillus and 0.53%-0.83% for Aeromonas.

3. The bacterial agent for treating wastewater containing benzalkonium chloride according to claim 2, characterized in that: The mass percentages of Pseudomonas, Achromobacter, Stenotrophomonas and Aeromonas were 32.37%, 14.69%, 4.65% and 0.53% respectively; the mass percentages of Rhizobium, Tistrina motilium, Flavobacterium, Alcaligenes, Acetogenic Proteophilus and Bacillus were 21.94%, 3.79%, 2.41%, 2.26%, 1.88% and 1.59% respectively.

4. An application of a bacterial agent for treating wastewater containing benzalkonium chloride according to claim 2, characterized in that: include: 5 g / L or 10 g / L of the bacterial agent is added to the wastewater containing benzalkonium chloride to be treated, and after anaerobic treatment at 28-34° C. for 5 days, aerobic treatment is performed.

5. The use of a bacterial agent for treating wastewater containing benzalkonium chloride according to claim 4, characterized in that: Also includes: The temperature is set during the treatment process, untreated benzalkonium chloride-containing wastewater is used to replace the treated benzalkonium chloride-containing wastewater mixture or its filtered supernatant, or nutrient salts or adsorption carriers are added during the treatment process.

6. The use of a bacterial agent for treating wastewater containing benzalkonium chloride according to claim 5, characterized in that: During the treatment process, the temperature was set at 32°C, and the untreated benzalkonium chloride-containing wastewater was used to replace the supernatant of the treated benzalkonium chloride-containing wastewater mixture every day, and 0.25-0.5 g / L of potassium dihydrogen phosphate was added every three days.

7. The use of a bacterial agent for treating wastewater containing benzalkonium chloride according to claim 5, characterized in that: During the treatment process, the temperature was set at 34°C, and the untreated benzalkonium chloride-containing wastewater was used to replace the supernatant of the treated benzalkonium chloride-containing wastewater mixture every day, and 0.25-0.5 g / L of potassium dihydrogen phosphate was added every three days.

8. The use of a bacterial agent for treating wastewater containing benzalkonium chloride according to claim 5, characterized in that: During the treatment process, the temperature was set at 34°C, and the supernatant of the treated benzalkonium chloride-containing wastewater mixture was replaced with untreated benzalkonium chloride-containing wastewater every three days, and 0.25-0.5 g / L of potassium dihydrogen phosphate was added every three days.

9. The use of a bacterial agent for treating wastewater containing benzalkonium chloride according to claim 5, characterized in that: During the treatment process, the temperature was set at 34°C, and the treated benzalkonium chloride-containing wastewater mixture was replaced with untreated benzalkonium chloride-containing wastewater every three days. 0.5 g / L of potassium dihydrogen phosphate and 5 g / L of activated carbon were added every three days.

10. The use of the bacterial agent for treating wastewater containing benzalkonium chloride according to claim 9, characterized in that: The COD removal rate reached 93.51%.

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