A chicory tea and a method for producing the same

Abstract

The present application belongs to the field of substitute tea quality and plant physiology, and particularly relates to a chicory tea and a production method thereof. The method is as follows: a chicory growth composition is applied to soil where chicory grows, and earthworm seedlings are put into the soil, and then chicory leaves are harvested to make chicory tea; the chicory growth composition is made of the following raw materials in parts by weight: 1-50 parts of nitrogen-fixing bacteria and 1-100 parts of natural plant fiber material with water absorption. The method provided by the present application effectively increases the content of chicory acid, and provides a new perspective for improving the quality of chicory substitute tea and increasing the content of chicory acid.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of environmental protection materials, soil improvement and functional tea, and particularly relates to a chicory tea and a production method thereof. BACKGROUND

[0002] Chicory (Cichorium intybus L.) is a perennial herbaceous plant of the genus Cichorium in the Asteraceae family, which has strong adaptability and can grow in various climatic conditions and ecological environments, and has important value in ecological restoration, soil and water conservation, etc. It has high nutritional value, rich in crude protein, crude fiber and crude fat, and is a good animal feed. At the same time, chicory has great significance in the medical field, and has the effects of clearing heat and detoxifying, promoting bile secretion, diuresis, swelling, stomachic and digestion, etc., and also has certain help for improving body resistance. Studies have shown that chicory granules can treat hyperuricemia, with a clinical total effective rate of 83.33% and an average reduction of serum uric acid by 23.41% after a treatment course of 1 month. It also has the effects of regulating blood lipids and protecting liver. However, the clinical efficacy and safety are not clear, and clinical research needs to be carried out for evaluation, and the mechanism of action needs to be further studied.

[0003] Chicoric acid is a kind of caffeic acid derivative mainly isolated from plants in the Asteraceae family, and is an important active ingredient in chicory. The relationship between chicoric acid and the quality improvement of chicory tea is as follows: (1) enhancing health care function: chicoric acid is an important active ingredient in chicory plants, and has various pharmacological activities such as improving body immunity, anti-tumor, anti-virus, anti-oxidation, reducing sugar and cholesterol, etc. These effects make chicory tea containing rich chicoric acid have significant advantages in promoting human health. (2) promoting the absorption of nutritional ingredients: chicoric acid, as an organic acid, helps to promote the absorption and utilization of other nutritional ingredients such as minerals and vitamins in tea. This is of great significance to improve the overall nutritional value of chicory tea. (3) increasing the stability and shelf life of tea: chicoric acid has certain antioxidant capacity, which can help slow down the oxidation process of other ingredients in tea, thereby maintaining the freshness and quality of tea. In addition, it also has certain inhibitory effect on the growth of microorganisms, which helps to prolong the shelf life of chicory tea.

[0004] At present, it is difficult to improve the content of chicoric acid in chicory tea during the production process of chicory tea. SUMMARY

[0005] To solve the above problems, the present application provides a chicory tea and a production method thereof to improve the content of chicoric acid.

[0006] The present application is realized by the following technical solutions:

[0007] A method for producing chicory tea comprises applying a chicory growth composition to soil where chicory is grown, placing earthworm seedlings in the soil, and harvesting chicory leaves after they grow to make chicory tea; the application amount of the chicory growth composition is 79.62 g / m 2 ~477.71g / m 2 The amount of earthworm seedlings released is 1 / m 2 ~10 pieces / m 2 .

[0008] The chicory growth composition is prepared from the following raw materials in parts by weight: 1 to 50 parts of nitrogen-fixing bacteria and 1 to 100 parts of natural plant fiber materials with water absorption performance.

[0009] Preferably, the chicory growth composition is made of the following raw materials in parts by weight: 1 to 15 parts of nitrogen-fixing bacteria and 5 to 60 parts of natural plant fiber materials with water absorption properties.

[0010] Preferably, the earthworm seedlings are at least one of Pheretima aspergillum, Pheretima carnosa and Shanghai Pheretima.

[0011] Preferably, the natural plant fiber material with water absorption performance is a water-absorbing bamboo fiber material.

[0012] Preferably, the preparation method of the water-absorbing bamboo fiber material comprises the following steps:

[0013] Use bamboo stems of 250-350 days old as materials, wash them, and grind them into bamboo powder of 75-85 mesh.

[0014] use 60 The water-absorbing bamboo fiber material is obtained by irradiating with a Co irradiation source and an irradiation dose of 15 kGy to 20 kGy at a temperature of 60 to 65 degrees Celsius for 5 to 6 minutes to change the primary molecular structure of the bamboo fiber.

[0015] Preferably, the nitrogen-fixing bacteria is rhizobium powder, and the concentration of live bacteria in the powder is 100 million CFU / g to 200 million CFU / g.

[0016] Preferably, the soil should maintain the following conditions before the earthworm seedlings are placed: the soil moisture content is maintained at 40% to 50%; the soil pH value is maintained at 6 to 8; and the soil temperature is maintained at 20°C to 25°C.

[0017] Preferably, before the earthworm seedlings are placed in the soil, they are first placed on the soil surface at a density of 1 / m 2 ~2 pieces / m 2The earthworm seedlings are put into the ditch with a depth of 8cm-12cm, and the number of the earthworm seedlings put into each ditch is 1-10.

[0018] Preferably, the production process of the chicory leaf for making chicory tea comprises the following steps:

[0019] The chicory fresh leaves are spread, the air temperature is 15-26℃, the humidity is 65%-85%, the leaves are continuously grabbed, and the moisture content is 55%-60% when the leaves are soft and moist.

[0020] In the rolling stage, the temperature is 20-26℃, the humidity is 85%-90%, the rolling time is 40-55min, and the strip is tight; after being taken out, the fermentation is carried out at a temperature of 25-27℃ and a humidity of 90%-100% for 2-2.5h, the leaves are dried, the temperature is increased to 90-100℃, and the fermentation is terminated when the temperature of the fermented leaves reaches more than 75℃; the chicory tea is obtained by drying at 85-95℃.

[0021] Preferably, the content of the chicory acid in the chicory tea is 0.30-0.55mg / mL.

[0022] Compared with the prior art, the present application has the following beneficial effects:

[0023] The present application provides a production method of chicory tea, wherein a chicory growth composition is applied to the soil where chicory grows, and earthworm seedlings are put into the soil, and the chicory leaves are harvested to make chicory tea; the application amount of the chicory growth composition is 79.62-477.71g / m 2 , the amount of the earthworm seedlings put into the soil is 1-10 per square meter, and the chicory growth composition is made of the following raw materials by weight fraction: 1-50 parts of nitrogen-fixing bacteria and 1-100 parts of natural plant fiber material with water absorption. 2 2 2 The present application considers the new material level, the microbial level and the field biological relationship level, and for the first time uses the combination of nitrogen-fixing bacteria, water absorption bamboo fiber material and earthworms to improve the content of chicory acid, and the three components are used in the above-mentioned proportion to produce chicory tea, which has a good effect on improving the content of chicory acid and significantly improves the content of chicory acid.

[0024] ​​In the present application, the provided chicory growth composition can be used in combination with earthworms to promote the growth of chicory and improve the quality of chicory. The fresh leaves are harvested to make chicory tea, and the selected chicory leaves are subjected to air and water walking, rolling and handling, which significantly reduces the bitterness and green taste of chicory itself, changes the taste of chicory tea, and makes chicory tea more palatable. The fermentation process increases aromatic substances, increases aroma, enhances the flavor and functionality of chicory tea. While increasing the content of chicory acid, the bitterness is reduced, and the taste is significantly improved. And has the advantages of low cost, no environmental pollution, and is suitable for wide application. It provides a new perspective for improving the quality of chicory tea and increasing the content of chicory acid. BRIEF DESCRIPTION OF DRAWINGS

[0025] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the drawings needed to be used in the embodiments or prior art description will be briefly introduced below. Obviously, the drawings in the following description are only some embodiments of the present application, and those skilled in the art can also obtain other drawings according to these drawings without creative labor.

[0026] Figure 1 The field map after the chicory growth composition and earthworm seedlings of the present application are put into the field. DETAILED DESCRIPTION

[0027] In order to facilitate the understanding of the present application, the present application will be described more fully below, and the preferred embodiments of the present application are given. However, the present application can be realized in many different forms, and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the disclosure of the present application more thorough and comprehensive.

[0028] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which the present application belongs. The terms used in the specification of the present application herein are only for the purpose of describing the specific embodiments, and are not intended to limit the present application.

[0029] The inventive concept of the present application is as follows:

[0030] Chicory acid is a kind of coffee acid derivative mainly isolated from plants in the family Asteraceae, and is an important active ingredient in chicory, which can enhance the health function, promote the absorption of nutritional ingredients, increase the stability and shelf life of tea. At present, how to use new materials, microbial technology and field production environment to improve and increase the content of chicory acid in chicory tea is a difficulty in the production process of chicory tea.

[0031] Based on this, the present invention provides a method for producing chicory tea, which comprises applying a chicory growth composition to soil where chicory grows, placing earthworm seedlings in the soil, and harvesting chicory leaves after they grow to make chicory tea; the application amount of the chicory growth composition is 79.62 g / m 2 ~477.71g / m 2 The amount of earthworm seedlings released is 1 / m 2 ~10 pieces / m 2 ; The chicory growth composition is made of the following raw materials in parts by weight: 1 to 50 parts of nitrogen-fixing bacteria and 1 to 100 parts of natural plant fiber materials with water absorption properties. The nitrogen-fixing bacteria and the natural plant fiber materials with water absorption properties are applied to the soil where chicory grows in the said proportion to increase and promote the population of soil earthworms, create a good environment for chicory growth, increase the content of chicoric acid in chicory, and harvest fresh leaves to make chicory tea. The selected chicory leaves are subjected to a process of drying, dewatering and rolling to significantly reduce the bitterness and green taste of the chicory itself, change the taste of the chicory tea, and make the chicory tea more palatable. The fermentation process increases aromatic substances, increases fragrance, and enhances the flavor and functionality of the chicory tea.

[0032] The technical solution of the present invention will be further described below with reference to specific embodiments.

[0033] Example 1: A method for producing chicory tea

[0034] Raw materials preparation:

[0035] (1) Nitrogen-fixing bacteria

[0036] Sinorhizobium meliloti powder, with a content of 100 million CFU / g, was provided by the Sichuan Key Laboratory of Bamboo Pest and Disease Control and Resource Development.

[0037] (2) Preparation of water-absorbent bamboo fiber

[0038] The bamboo stems grown for 250 days at the Bamboo Sea Base in Yongfu Town, Muchuan County were used as the raw materials. After being washed with tap water, they were ground into 75 mesh fine bamboo powder. 60 Co irradiation source, irradiation dose of 15kGy, temperature at 60℃, irradiation treatment for 5min, achieved directional modification of bamboo fiber, and obtained water-absorbent bamboo fiber material with 100 times higher water absorption.

[0039] (3) Earthworms

[0040] The earthworms Pheretima aspergillum were selected, each weighing 5 g.

[0041] The soil of the test land is located in Angu Town, Leshan City, Sichuan Province. The area of ​​the test land is 120m 2The test land is divided into 6 areas, wherein the test treatment area is 20m 2 , repeated 3 times; the control area is 20m 2 , repeated 3 times.

[0042] The soil surface of the test land is evenly spread with the chicory growth composition, which is made of 1 part of nitrogen-fixing bacteria and 1 part of water-absorbing bamboo fiber material by weight fraction. The application amount of the chicory growth composition in each area is 79.62g / m 2 , each area is watered with 100kg to keep the soil moist to ensure the suitable living environment for earthworms, i.e. to maintain the following conditions: the soil moisture content is kept at 40%, the soil pH value is 6, and the soil temperature is kept at 20℃. An 8cm deep ditch is opened on the soil surface every 1m 2 , and 1 earthworm seedling is put into each ditch and covered with soil. In the control area, only 60g / m 2 of nitrogen-fixing bacteria is used in each area without using other materials, each area is watered with 100kg, and the field map after the chicory growth composition and earthworm seedlings are put in is shown in Figure 1 . The chicory leaves are harvested 15 days after the leaves grow.

[0043] The harvested chicory fresh leaves are placed in a wilting room with constant temperature of 15℃ and humidity of 65%, and are turned over every hour for 5h, and are continuously grabbed for 48h until the leaves are soft and the moisture content is 55%, completing the wilting and water removal;

[0044] The wilting and water removal stage is followed by the rolling stage, and the rolling room is kept at constant temperature of 20℃ and humidity of 85%, and the rolling is performed for 40min according to the light-heavy-light principle, and the strips are tightly knitted. The rolled leaves are taken out and placed in a bamboo basket and moved into a fermentation room, which is kept at constant temperature of 25℃ and humidity of 90%, and the fermentation is performed for 2h, during which the chicory has a sweet smell and the green smell disappears, and the leaf color is dark green. The fermentation is completed, and the fermented leaves are dried by rapidly increasing the temperature to 90℃ with a rough fire for 10min to make the temperature of the fermented leaves reach above 75℃ to terminate the fermentation. Then the fermented leaves are poured into a winnowing basket to cool down and the thick and old leaves are picked out, and the leaves are put into a drying cabinet after 1h to be dried at 85℃ to obtain the chicory tea.

[0045] Determination of the content of chicory acid:

[0046] (1) Determination method

[0047] The content of chicory acid is determined by high performance liquid chromatography (HPLC).

[0048] Preparation of the reference solution: an appropriate amount of chicory acid reference substance is taken and 80% methanol is added to prepare a solution containing 0.2mg of chicory acid per 1mL.

[0049] Preparation of test solution: take 0.5 g of sample powder (pass through No. 4 sieve), put into a conical flask with plug, add 20 mL of 80% methanol, weigh, ultrasonic treat (power 400 W, frequency 40 kHz) for 20 min, cool, re-weigh, make up the weight loss with 80% methanol, shake well, filter, take the filtrate, and obtain the test solution.

[0050] Determination of chlorogenic acid content: take 10 μL of the control solution and the test solution respectively, inject into the liquid chromatograph, record the chromatogram, and calculate the content by peak area according to the external standard method.

[0051] (2) Determination results

[0052] Randomly sample the produced chicory tea leaves in each area three times, determine the content of chlorogenic acid by HPLC method, and repeat each sample three times. The research results show that the content of chlorogenic acid is 0.55 mg / mL. Compared with the control, the content of chlorogenic acid in the area where the growth composition of chicory and earthworms are applied is obviously increased by 31.5%.

[0053] Example 2, a production method of chicory tea.

[0054] Raw material preparation:

[0055] (1) nitrogen-fixing bacteria

[0056] The Sinorhizobium meliloti bacterial powder has Latin name Sinorhizobium meliloti and a content of 0.2 billion CFU / g, which is provided by Sichuan Key Laboratory of Bamboo Pest and Disease Control and Resource Development.

[0057] (2) Preparation of water-absorbing Bambusa emeiensis fiber

[0058] Take the 300-day-old Bambusa emeiensis stems grown in the bamboo base of Yongfu Town in Muchuan as the material, wash with tap water, grind into fine bamboo powder with fineness of 80 meshes, and use 60 Co irradiation source and irradiation dose of 20 kGy, irradiate for 6 min at a temperature of 65℃, realize directional modification of the bamboo fiber, and obtain water-absorbing Bambusa emeiensis fiber material with water absorption of 100 times.

[0059] (3) Earthworms

[0060] Pheretima carnosa is selected, and each has a weight of 5 g.

[0061] The soil of the test land is in An Valley Town, Leshan City, Sichuan Province, and the area of the test land is 120 m 2 . The test land is divided into six areas, wherein the test treatment area is 20 m 2 , repeated three times; the control area is 20 m 2 , repeated three times.

[0062] The chicory growth composition was evenly spread on the soil surface of the test land. The chicory growth composition was made of the following raw materials by weight: 1 part of nitrogen-fixing bacteria and 5 parts of water-absorbing bamboo fiber material. The application rate of the chicory growth composition in each area was 79.62g / m 2 , water each area with 100kg of water to keep the soil moist to ensure a suitable living environment for earthworms, that is, maintain the following conditions: soil moisture content is maintained at 45%, soil pH is 7, and soil temperature is maintained at 23℃. 2 A 10cm deep trench was dug, and two earthworm seedlings were placed in each trench and covered with soil. In the control area, only nitrogen-fixing bacteria 60g / m 2 , without using other materials, water each area with 100kg of water. Harvest and make tea 15 days after the chicory leaves grow.

[0063] The harvested chicory leaves are placed in a thin layer in the withering room. The temperature is kept constant at 15°C and the humidity is 65%. After 5 hours, they are gently turned over every hour. After 48 hours, the leaves are constantly picked, soft, dark in color, and have a moisture content of 55%, which means they are completely withered.

[0064] Entering the rolling stage, the temperature of the rolling room is constant at 20℃ and the humidity is 85%. According to the light-heavy-light principle, roll for 40 minutes until the strips are tight; take out, put in a bamboo basket and move to the fermentation room. The temperature of the fermentation room is constant at 25℃ and the humidity is 90%. Ferment for 2 hours. The chicory has a sweet aroma, the green smell dissipates, the leaves are dark green, and the fermentation is in place. Dry it and quickly heat it to 90℃ with a rough fire for 10 minutes to let the temperature of the fermented leaves reach above 75℃ to terminate the fermentation; then pour it into a dustpan to cool and pick out the coarse old leaves. After 1 hour, put it into the drying cabinet and dry it at 85℃ to obtain chicory tea.

[0065] Determination of chicoric acid content:

[0066] The chicoric acid content was determined using the chicoric acid determination method described in Example 1. Three random samples of chicoric acid tea leaves from each region were collected and assayed using HPLC, with each sample replicated three times. The results showed a chicoric acid content of 0.40 mg / mL. Compared to the control, the chicoric acid content in the region treated with the chicoric acid growth composition and earthworms increased significantly, by 18.3%.

[0067] Example 3: A method for producing chicory tea.

[0068] Raw materials preparation:

[0069] (1) Nitrogen-fixing bacteria

[0070] Sinorhizobium meliloti, Latin name, content of 0.1 billion CFU / g, provided by Sichuan Provincial Key Laboratory of Bamboo Pest Control and Resource Development.

[0071] (2) Preparation of water-absorbing Dendrocalamopsis hedyschoides fiber

[0072] The 350-day-old Dendrocalamopsis hedyschoides stem grown in the bamboo base of Yongfu Town in Muchuan County was used as the material. After being washed with tap water, the bamboo was ground into fine bamboo powder with a fineness of 85 mesh. The fine bamboo powder was treated with 60 Co irradiation source, irradiation dose of 20 kGy, irradiation treatment for 6 min at a temperature of 65°C, realizing the directional modification of bamboo fiber, and obtaining water-absorbing Dendrocalamopsis hedyschoides fiber material with 100 times water absorption.

[0073] (3) Earthworms

[0074] Shanghai Pheretima was selected, and each had a weight of 5 g.

[0075] The soil of the test land was in An Valley Town, Leshan City, Sichuan Province, and the area of the test land was 120 m 2 . The test land was divided into six areas, of which the test treatment area was 20 m 2 , repeated three times; the control area was 20 m 2 , repeated three times.

[0076] The chicory growth composition was evenly spread on the surface of the soil of the test land, and the chicory growth composition was made from the following raw materials in parts by weight: nitrogen-fixing bacteria 15 parts, water-absorbing Dendrocalamopsis hedyschoides fiber material 60 parts. The application amount of chicory growth composition in each area was 79.62 g / m 2 , each area was watered with 100 kg to keep the soil moist, so as to ensure the suitable living environment for earthworms, i.e. to maintain the following conditions: soil moisture content of 50%, soil pH value of 8, and soil temperature of 25°C. A 12 cm deep trench was opened on the surface of the soil every 1 m 2 , and 3 earthworm seedlings were put into each trench and covered with soil. In the control area, only 60 g / m 2 of nitrogen-fixing bacteria was used in each area without using other materials, and each area was watered with 100 kg. The harvested chicory leaves were placed in the wilting room after 15 days of leaf growth.

[0077] The harvested chicory leaves were placed in the wilting room, the air temperature was kept at 15°C, and the humidity was 65%. After 5 hours, the leaves were turned over every hour, and after 48 hours, the leaves were continuously grabbed, soft and dark, with a moisture content of 55%, completing the wilting and water removal;

[0078] Into the rubbing stage, the rubbing room temperature is constant at 20℃, humidity 85%, according to light-heavy-light principle, rubbing 40 minutes, strip tight knot; take out and put in the bamboo basket into the fermentation room, the fermentation room temperature is constant at 25℃, humidity is 90%, fermentation 2h, the chichorium intybus appears sweet smell, green smell away, leaf color dark green, fermentation to place, drying, to the hair fire rapid heating to 90℃, 10min, let the fermentation leaf temperature reaches more than 75℃, end fermentation; then pour into the winnowing basket and pick up the old leaves, 1h after put into the drying cabinet, 85℃ drying to get chichorium intybus tea.

[0079] Determination of chichorium intybus acid content:

[0080] According to the determination method of chichorium intybus acid content described in example 1, the chichorium intybus tea leaves prepared in each area are randomly sampled three times, and the chichorium intybus acid content is determined by HPLC method, and each sample is repeated three times. The research results show that the chichorium intybus acid content is 0.49mg / mL, compared with the control, the chichorium intybus acid in the area where the chichorium intybus growth composition and earthworms are applied is obviously increased, increased by 20.5%.

[0081] Example 4, a production method of chichorium intybus tea.

[0082] Raw material preparation:

[0083] (1) nitrogen-fixing bacteria

[0084] The Sinorhizobium meliloti, Latin name, contains 0.2 billion CFU / g, which is provided by Sichuan Provincial Key Laboratory of Bamboo Pest and Disease Control and Resource Development.

[0085] (2) preparation of water-absorbing bambusa emeiensis fiber

[0086] The 300d-old b. emeiensis stems grown in the bamboo base of Yongfu Town, Muchuan County, are washed with tap water, ground into 80 mesh fine bamboo powder, and then treated with 60 Co irradiation source, irradiation dose 15kGy, irradiation treatment 5min at temperature 60℃, realizing the directional modification of bamboo fiber, obtaining water-absorbing b. emeiensis fiber material with 100 times water absorption.

[0087] (3) earthworms

[0088] Pheretima aspergillum is selected, each weighing 6g.

[0089] The soil of the test land is in An Valley Town, Leshan City, Sichuan Province, and the area of the test land is 120m 2 . The test land is divided into 6 areas, of which the test treatment area is 20m 2 , repeated 3 times; the control area is 20m 2 , repeated 3 times.

[0090] The soil surface of the test land is evenly spread with the chicory growth composition, which is made of 50 parts of nitrogen-fixing bacteria and 100 parts of water-absorbing Neosinocalamus Goscinskii fiber material by weight fraction. The application amount of the chicory growth composition in each area is 300 g / m 2 Each area is watered with 100 kg of water to keep the soil moist, so as to ensure the suitable living environment for earthworms, i.e. to keep the following conditions: the soil moisture content is kept at 40%, the soil pH value is 6, and the soil temperature is kept at 20°C. Every 2 / m 2 An 8-cm-deep ditch is dug on the soil surface, and 4 earthworm seedlings are put into each ditch before covering the soil. In the control area, only 60 g / m 2 of nitrogen-fixing bacteria is used in each area without using other materials, and each area is watered with 100 kg of water. The chicory leaves are harvested 15 days after the leaves grow.

[0091] The harvested chicory fresh leaves are thinly spread in the wilting room, the air temperature is kept at 20°C, and the humidity is 75%. After 7.5 hours, the leaves are turned over every hour, and after 48 hours, the leaves are continuously grabbed, soft and dark, and the water content is 58%, completing the wilting and water removal.

[0092] The rolling stage is entered, the rolling room temperature is kept at 24°C, and the humidity is 87%. According to the light-heavy-light principle, the rolling time is 50 minutes, and the strip is tight. After being taken out and placed in a bamboo basket, the fermentation room temperature is kept at 26°C, and the humidity is 95%. After 2.3 hours of fermentation, the chicory has a sweet smell, the green smell disappears, and the leaf color is dark green. The fermentation is in place, and the fermented leaves are dried by quickly increasing the temperature to 95°C for 12 minutes to stop the fermentation. Then, the leaves are poured into a winnowing basket to cool down and the thick and old leaves are picked out. After 1 hour, the leaves are placed in an oven cabinet and dried at 90°C to obtain chicory tea.

[0093] Determination of chicoric acid content:

[0094] According to the determination method of chicoric acid content described in Example 1, the chicory tea leaves prepared in each area are randomly sampled three times, the chicoric acid content is determined by HPLC method, and each sample is repeated three times. The research results show that the chicoric acid content is 0.40 mg / mL. Compared with the control, the chicoric acid in the area where the chicory growth composition and earthworms are applied is obviously increased by 12.5%.

[0095] Example 5, a production method of chicory tea.

[0096] Raw material preparation:

[0097] (1) Nitrogen-fixing bacteria

[0098] Sinorhizobium meliloti, Latin name, content of 0.2 billion CFU / g, provided by Sichuan Provincial Key Laboratory of Bamboo Pest Control and Resource Development.

[0099] (2) Preparation of water-absorbing Dendrocalamopsis hedyschoides fiber

[0100] The 300-day-old Dendrocalamopsis hedyschoides stem grown in the bamboo base of Yongfu Town in Muchuan County was washed with tap water and ground into fine bamboo powder with a fineness of 80 mesh. The fine bamboo powder was mixed with 60 Co irradiation source, irradiation dose of 15 kGy, irradiation treatment for 5 min at a temperature of 60°C, realizing the directional modification of bamboo fiber, and obtaining water-absorbing Dendrocalamopsis hedyschoides fiber material with 100 times water absorption.

[0101] (3) Earthworms

[0102] Pheretima aspergillum was selected, and each worm weighed 6 g.

[0103] The soil of the test land was in An Valley Town, Leshan City, Sichuan Province, and the area of the test land was 120 m 2 . The test land was divided into 6 areas, of which the test treatment area was 20 m 2 , repeated 3 times; the control area was 20 m 2 , repeated 3 times.

[0104] The chicory growth composition was evenly spread on the surface of the soil of the test land, and the chicory growth composition was made from the following raw materials in parts by weight: nitrogen-fixing bacteria 1 part, water-absorbing Dendrocalamopsis hedyschoides fiber material 5 parts. The application amount of chicory growth composition in each area was 300 g / m 2 , and each area was watered with 100 kg to keep the soil moist to ensure the suitable living environment for earthworms, i.e. to maintain the following conditions: soil moisture content of 40%, soil pH value of 6, and soil temperature of 20°C. A 10 cm deep ditch was opened on the surface of the soil every 2 m 2 , and 6 earthworm seedlings were put into each ditch and covered with soil. In the control area, only 60 g / m 2 of nitrogen-fixing bacteria was used in each area without using other materials, and each area was watered with 100 kg. The chicory leaves were harvested 15 days after the leaves grew to make tea.

[0105] The harvested chicory fresh leaves were placed in the wilting room, the air temperature was kept at 20°C, and the humidity was 75%. After 7.5 h, the leaves were turned over every hour, and after 48 h, the leaves were continuously grabbed, soft and pale, with a water content of 58%, completing the wilting and water removal;

[0106] Into the rubbing stage, the rubbing room temperature is constant at 24℃, humidity 87%, according to light-heavy-light principle, rubbing 50 min, strip tight knot; take out and move into fermentation room in bamboo basket, fermentation room temperature is constant at 26℃, humidity is 95%, fermentation 2.3h, chichorium intybus appears sweet smell, green smell disappears, leaf color dark green, fermentation in place, drying, to hair fire fast heating to 95℃, lasting 12 min, let fermentation leaf temperature reach above 75℃, end fermentation; then pour into winnowing basket, spread and pick coarse old leaves, 1h later, put into drying cabinet, 90℃ drying, chichorium intybus tea is obtained.

[0107] Determination of chichorium intybus acid content:

[0108] According to the determination method of chichorium intybus acid content described in example 1, chichorium intybus tea leaves prepared in each area are randomly sampled three times, chichorium intybus acid content is determined by HPLC method, each sample is repeated three times. The research results show that: chichorium intybus acid content is 0.38mg / mL, compared with the control, chichorium intybus acid in the area where earthworms and chichorium intybus growth composition are applied is obviously increased, increased by 10.3%.

[0109] Example 6, a production method of chichorium intybus tea.

[0110] Raw material preparation:

[0111] (1) nitrogen-fixing bacteria

[0112] Alfalfa Sinorhizobium meliloti powder, Latin name is Sinorhizobium meliloti, content is 0.1 billion CFU / g, provided by Sichuan Provincial Key Laboratory of Bamboo Pest and Disease Control and Resource Development.

[0113] (2) preparation of water-absorbing bambusa emeiensis fiber

[0114] Take the bambusa emeiensis stem grown in the bamboo base of Yongfu Town, Muchuan for 300d as material, wash with tap water, grind into 80 mesh fine bamboo powder, use 60 Co irradiation source, irradiation dose 15kGy, irradiation treatment 5min at temperature 60℃, realize directional modification of bamboo fiber, obtain water-absorbing 100 times water-absorbing bambusa emeiensis fiber material.

[0115] (3) earthworm

[0116] Select Pheretima aspergillum, each weight 6g.

[0117] The soil of the test land is in An Valley Town, Leshan City, Sichuan Province, the area of the test land is 120m 2 . The test land is divided into 6 areas, among them, the test treatment area is 20m 2 , repeated 3 times; the control area is 20m 2 , repeated 3 times.

[0118] The soil surface of the test land is evenly spread with the chicory growth composition, which is made of 1 part of nitrogen-fixing bacteria and 5 parts of water-absorbing Neosinocalamus Goidackeri fiber material by weight fraction. The application amount of the chicory growth composition in each area is 300 g / m 2 Each area is watered with 100 kg of water to keep the soil moist to ensure that the suitable living environment for earthworms is reached, i.e. the following conditions are maintained: the soil moisture content is kept at 40%, the soil pH value is 6, and the soil temperature is kept at 20°C. On the soil surface, 8 earthworm seedlings are placed in each 1 / m 2 Deep 12 cm trenches are dug, and 8 earthworm seedlings are placed in each trench before covering the soil. In the control area, only 60 g / m 2 of nitrogen-fixing bacteria is used in each area without using other materials, and each area is watered with 100 kg of water. The chicory leaves are harvested 15 days after the leaves grow.

[0119] The harvested chicory fresh leaves are thinly spread in the wilting room, the air temperature is kept constant at 20°C, and the humidity is 75%. After 7.5 hours, the leaves are turned over every hour, and after 48 hours, the leaves are continuously grabbed, soft and dark, and the water content is 58%, completing the wilting and water removal;

[0120] The rolling stage is entered, the rolling room temperature is kept constant at 24°C, and the humidity is 87%. According to the light-heavy-light principle, the rolling time is 50 minutes, and the strip is tight. After being taken out and placed in a bamboo basket, it is moved into the fermentation room, the fermentation room temperature is kept constant at 26°C, and the humidity is 95%. After 2.3 hours of fermentation, the chicory has a sweet aroma and the green smell disappears, the leaf color is dark green, and the fermentation is in place. Drying is performed by quickly raising the temperature to 95°C with a rough fire for 12 minutes to allow the fermentation leaf temperature to reach above 75°C to terminate fermentation. Then, it is poured into a winnowing basket to cool and pick out thick and old leaves. After 1 hour, it is placed in an oven cabinet and dried at 90°C to obtain chicory tea.

[0121] Determination of chicory acid content:

[0122] According to the determination method of chicory acid content described in Example 1, the chicory tea leaves prepared in each area are randomly sampled three times, and the chicory acid content is determined by HPLC method, with each sample repeated three times. The research results show that the chicory acid content is 0.36 mg / mL. Compared with the control, the chicory acid in the area where the chicory growth composition and earthworms are applied is significantly increased by 7%.

[0123] Example 7, a production method of chicory tea.

[0124] Raw material preparation:

[0125] (1) Nitrogen-fixing bacteria

[0126] Sinorhizobium meliloti, Latin name, content of 0.2 billion CFU / g, provided by Sichuan Provincial Key Laboratory of Bamboo Pest Control and Resource Development.

[0127] (2) Preparation of water-absorbing Dendrocalamopsis hedyschoides fiber

[0128] The 300-day-old Dendrocalamopsis hedyschoides stem grown in the bamboo base of Yongfu Town, Muchuan County was used as the material. After being washed with tap water, the bamboo was ground into fine bamboo powder with a fineness of 80 mesh. The fine bamboo powder was mixed with 60 Co irradiation source, irradiation dose of 15 kGy, irradiation treatment for 5 min at a temperature of 60°C, the directional modification of bamboo fiber was realized, and the water-absorbing Dendrocalamopsis hedyschoides fiber material with 100 times water absorption was obtained.

[0129] (3) Earthworms

[0130] Pheretima aspergillum was selected, and each worm weighed 7 g.

[0131] The soil of the test land was in An'gu Town, Leshan City, Sichuan Province, and the area of the test land was 120 m 2 . The test land was divided into 6 areas, of which the test treatment area was 20 m 2 , repeated 3 times; the control area was 20 m 2 , repeated 3 times.

[0132] The chicory growth composition was evenly spread on the surface of the soil of the test land, and the chicory growth composition was made from the following raw materials in parts by weight: nitrogen-fixing bacteria 1 part, water-absorbing Dendrocalamopsis hedyschoides fiber material 5 parts. The application amount of the chicory growth composition in each area was 477.71 g / m 2 , each area was watered with 100 kg to keep the soil moist to ensure the suitable living environment for earthworms, i.e. to maintain the following conditions: soil moisture content of 40%, soil pH value of 6, and soil temperature of 20°C. An 8 cm deep ditch was dug on the surface of the soil every 1 m 2 , and 9 earthworm seedlings were put into each ditch and covered with soil. In the control area, only 60 g / m 2 of nitrogen-fixing bacteria was used in each area without using other materials, and each area was watered with 100 kg. The chicory leaves were harvested 15 days after the leaves grew to make tea.

[0133] The harvested chicory fresh leaves were placed in the wilting room, the air temperature was kept at 26°C, and the humidity was 85%. After 8.5 hours, the leaves were turned over every hour, and after 48 hours, the leaves were continuously grabbed, soft and dark, with a water content of 60%, completing the wilting and water removal;

[0134] Into the rubbing stage, the rubbing room temperature is constant at 26℃, humidity 90%, according to light-heavy-light principle, rubbing 55min, strip tight knot; take out and move into fermentation room, fermentation room temperature is constant at 27℃, humidity 100%, fermentation 2.5h, chichorium intybus appears sweet smell, green smell disappears, leaf color dark green, fermentation in place, drying, to hair fire fast heating to 100℃, lasting 15min, let fermentation leaf temperature reach above 75℃, end fermentation; then pour into winnowing basket, spread and pick coarse old leaves, 1h later, put into drying cabinet, 95℃ drying, chichorium intybus tea is obtained.

[0135] Determination of chichorium intybus acid content:

[0136] According to the determination method of chichorium intybus acid content described in example 1, chichorium intybus tea leaves prepared in each area are randomly sampled three times, chichorium intybus acid content is determined by HPLC method, each sample is repeated three times. The research results show that: chichorium intybus acid content is 0.37mg / mL, compared with the control, chichorium intybus acid in the area where the chichorium intybus growth composition and earthworms are applied is obviously increased, increased by 7.3%.

[0137] Example 8, a production method of chichorium intybus tea.

[0138] Raw material preparation:

[0139] (1) nitrogen-fixing bacteria

[0140] Alfalfa Sinorhizobium meliloti powder, Latin name is Sinorhizobium meliloti, content is 0.1 billion CFU / g, provided by Sichuan Provincial Key Laboratory of Bamboo Pest and Disease Control and Resource Development.

[0141] (2) preparation of water-absorbing bambusa emeiensis fiber

[0142] Take the bambusa emeiensis stem grown in the bamboo base of Yongfu Town, Muchuan for 300d as material, wash with tap water, grind into 80 mesh fine bamboo powder, use 60 Co irradiation source, irradiation dose 15kGy, irradiation treatment 5min at temperature 60℃, realize directional modification of bamboo fiber, obtain water-absorbing 100 times of water-absorbing bambusa emeiensis fiber material.

[0143] (3) earthworm

[0144] Select Pheretima aspergillum, each weight 7g.

[0145] The soil of the test land is in An Valley Town, Leshan City, Sichuan Province, the area of the test land is 120m 2 . The test land is divided into 6 areas, among them, the test treatment area is 20m 2 , repeated 3 times; the control area is 20m 2 , repeated 3 times.

[0146] The chicory growth composition was evenly spread on the soil surface of the test land. The chicory growth composition was made of the following raw materials by weight: 1 part of nitrogen-fixing bacteria and 5 parts of water-absorbing bamboo fiber material. The application rate of the chicory growth composition in each area was 477.71g / m 2 , water each area with 100kg of water to keep the soil moist to ensure a suitable living environment for earthworms, that is, maintain the following conditions: soil moisture content is maintained at 40%, soil pH is 6, and soil temperature is maintained at 20℃. 2 8cm deep trenches were dug, 10 earthworm seedlings were placed in each trench and then covered with soil. In the control area, only nitrogen-fixing bacteria 60g / m 2 , without using other materials, water each area with 100kg of water. Harvest and make tea 15 days after the chicory leaves grow.

[0147] The harvested chicory leaves are placed in a withering room and spread thinly. The temperature is kept constant at 26°C and the humidity is 85%. After 8.5 hours, they are gently turned over every hour. After 48 hours, the leaves are constantly picked, soft, dark in color, and have a moisture content of 60%, which means they are completely withered.

[0148] Entering the rolling stage, the temperature in the rolling room is constant at 26°C and the humidity is 90%. According to the light-heavy-light principle, the rolling is carried out for 55 minutes, and the strips are tightly tied; take out, put in a bamboo basket and move into the fermentation room. The temperature in the fermentation room is constant at 27°C and the humidity is 100%. After fermentation for 2.5 hours, the chicory has a sweet aroma, the green smell dissipates, the leaves are dark green, and the fermentation is in place. Dry it, quickly heat it to 100°C with a rough fire, and continue for 15 minutes to let the temperature of the fermented leaves reach above 75°C, and terminate the fermentation; then pour it into a dustpan to cool and pick out the coarse old leaves. After 1 hour, put it into the drying cabinet and dry it at 95°C to obtain chicory tea.

[0149] Determination of chicoric acid content:

[0150] The chicoric acid content was determined using the method described in Example 1. Three random samples of chicoric acid tea leaves from each region were collected and assayed using HPLC, with each sample replicated three times. The results showed a chicoric acid content of 0.35 mg / mL. Compared to the control, the chicoric acid content in the region treated with the chicory growth composition and earthworms increased significantly, by 5.6%.

[0151] Example 9: A method for producing chicory tea.

[0152] Raw materials preparation:

[0153] (1) Nitrogen-fixing bacteria

[0154] Sinorhizobium meliloti, Latin name, content of 0.1 billion CFU / g, provided by Sichuan Provincial Key Laboratory of Bamboo Pest Control and Resource Development.

[0155] (2) Preparation of water-absorbing Dendrocalamopsis hedyschoides fiber

[0156] The 300-day-old Dendrocalamopsis hedyschoides stems grown in the bamboo base of Yongfu Town in Muchuan County were washed with tap water and ground into fine bamboo powder with a fineness of 80 mesh. 60 Co irradiation source, irradiation dose 15 kGy, irradiation treatment for 5 min at a temperature of 60°C, realizing the directional modification of bamboo fiber, and obtaining water-absorbing Dendrocalamopsis hedyschoides fiber material with 100 times water absorption.

[0157] (3) Earthworms

[0158] Pheretima aspergillum was selected, and each worm weighed 7 g.

[0159] The soil of the test land was in An Valley Town, Leshan City, Sichuan Province, and the area of the test land was 120 m 2 . The test land was divided into 6 areas, of which the test treatment area was 20 m 2 , repeated 3 times; the control area was 20 m 2 , repeated 3 times.

[0160] The chicory growth composition was evenly spread on the surface of the soil of the test land, and the chicory growth composition was made from the following raw materials in parts by weight: nitrogen-fixing bacteria 1 part, water-absorbing Dendrocalamopsis hedyschoides fiber material 5 parts. The application amount of chicory growth composition in each area was 477.71 g / m 2 , each area was watered with 100 kg to keep the soil moist, so as to ensure the suitable living environment for earthworms, i.e. to maintain the following conditions: soil moisture content of 40%, soil pH value of 6, and soil temperature of 20°C. An 8 cm deep ditch was opened on the surface of the soil every 1 m 2 , and 1 earthworm seedling was put into each ditch and covered with soil. In the control area, only 60 g / m 2 of nitrogen-fixing bacteria was used in each area without using other materials, and each area was watered with 100 kg. The chicory leaves were harvested for tea making after 15 days of leaf growth.

[0161] The harvested chicory fresh leaves were placed in the wilting room, the air temperature was kept at 26°C, and the humidity was 85%. After 8.5 hours, the leaves were turned over every hour, and after 48 hours, the leaves were continuously grabbed, soft and pale, with a water content of 60%, completing the wilting and water removal;

[0162] Into the rubbing stage, the rubbing room temperature is constant at 26℃, humidity 90%, according to light-heavy-light principle, rubbing 55 min, strip tight knot; take out and move into the fermentation room in the bamboo basket, the fermentation room temperature is constant at 27℃, humidity 100%, fermentation 2.5h, chrysanthemum appears sweet smell, green smell disappears, leaf color dark green, fermentation is in place, dry, with hair fire to quickly heat to 100℃, last 15 min, let the fermentation leaf temperature reach above 75℃, end fermentation; then pour into the winnowing basket and spread cool and pick coarse old leaves, 1h later put into the drying cabinet, 95℃ dry to obtain chrysanthemum tea.

[0163] Determination of chrysanthemum acid content:

[0164] According to the determination method of chrysanthemum acid content described in example 1, the chrysanthemum tea leaves prepared in each area are randomly sampled three times, and the chrysanthemum acid content is determined by HPLC method, and each sample is repeated three times. The research results show that the chrysanthemum acid content is 0.40 mg / mL, compared with the control, the chrysanthemum acid in the area where the chrysanthemum growth composition and earthworms are applied is obviously increased by 15.5%.

[0165] From the above results, it can be seen that the method for improving the quality of chrysanthemum substitute tea and increasing the content of chrysanthemum acid in the present test has achieved remarkable results, and the content of chrysanthemum acid in the test treatment area has been successfully increased. Compared with the control area, the chrysanthemum acid content in the different treatment areas is increased by more than 5%, which shows that the production method of chrysanthemum tea and the method for increasing the content of chrysanthemum acid are effective and feasible.

[0166] Experimental example 10, effect study

[0167] In order to improve the quality of chrysanthemum substitute tea and promote the increase of chrysanthemum acid content, the chrysanthemum growth composition and earthworms are taken as the object, and a comparative test is conducted. The specific research is as follows:

[0168] The effect study test is carried out in an artificial climate room in the Sichuan Leshan High-tech Zone laboratory, and the temperature is set and maintained at 23℃, the humidity is 70%, and the simulated day and night light is simulated.

[0169] Chrysanthemum is planted in a cylindrical plastic barrel with a diameter of 40 cm and a height of 30 cm. The planting conditions are as follows: the soil moisture content is maintained at 40%-50%; the pH value of the soil is 6-8; and the temperature of the soil is maintained at 20℃-25℃.

[0170] A one-way regression orthogonal design is used, and the design and implementation scheme is as shown in table 1 and table 2. According to the test research scheme, the nitrogen-fixing bacteria and water-absorbing bambusa emeiensis fiber material are weighed and placed on the surface of the barrel, 10 cm of holes are opened in each barrel, earthworms are put in, and the soil is covered 5 cm.

[0171] It should be noted that the top of the barrel is sealed with a gauze to prevent the earthworms from escaping.

[0172] The soil was watered with 30 ml of purified water every 10 days, and soil moisture was measured every 10 days to maintain a constant humidity of 50%. Chicory leaves were harvested 15 days after growth for tea production. The chicoric acid content was measured, and the chicoric acid increase rate was calculated. The results are shown in Table 2. SPSS 20.0 data analysis software was used for statistical analysis and significance testing.

[0173] Table 1 Orthogonal factor level design table L 9 (3 3 )

[0174]

[0175] Table 2 Orthogonal test design and results

[0176]

[0177]

[0178] It can be seen from Table 2 that the increasing rate of chicoric acid content at different experimental factor levels is different, among which the increasing rate of chicoric acid content at level factor 1, i.e. Z1 nitrogen-fixing bacteria 10 g / m 2 , Z2 water-absorbent bamboo fiber material 50g / m 2 , Z3 earthworm seedlings 8 pieces / m 2 The increasing rate of chicoric acid content was the highest.

[0179] Based on the data in Table 2, we calculated the regression coefficients using SPSS 20.0 and established a regression equation. Assuming that the rate of increase in chicoric acid content due to chicory tea is Y, and the influencing factors are Z1, Z2, and Z3, respectively, the regression equation is: Y = 14.0 + 6.6Z1 + 2.7Z2 + 3.3Z3.

[0180] According to the above regression equation, all regression coefficients are positive, that is, the effects of Z1 nitrogen-fixing bacteria, Z2 water-absorbing bamboo fiber, and Z3 earthworm seedlings on the increase rate and decrease rate of chicoric acid content Y are positively correlated, and the increase rate of chicoric acid content Y is the result of the joint action of the three factors. After the significance test, the effects of the three factors on Y: Z1 reached the extremely significant level of 0.01 with a P value of 0.000, and Z2 and Z3 both reached the significant level of 0.05, with P values ​​of 0.031 and 0.012 respectively; the significance test of the regression equation was carried out, and the multivariate correlation coefficient between Y and Z1, Z2, and Z3 was 0.938, and the regression equation reached the extremely significant level of 0.01 with a P value of 0.000.

[0181] Therefore, it can be seen that the nitrogen-fixing bacteria Z1, the water-absorbing bamboo fiber Z2 and the earthworm seedling Z3 have a significant correlation with the reduction rate of the chicoric acid content increase rate, and the regression equation is true and reasonable.

[0182] From the above results, it can be seen that the three materials each play a unique and significant role in increasing the chicoric acid content of the chicory tea, and the chicoric acid content is related to the nitrogen-fixing bacteria, water-absorbing bamboo fiber and earthworm seedlings. The combined use of the three materials can further improve the chicoric acid content of the chicory tea, and provides a new idea and method for improving the function of the chicory tea.

[0183] From the above results, it can be seen that the production method of the chicory tea and the method for improving the chicoric acid content provided by the present application use the nitrogen-fixing bacteria, the water-absorbing bamboo fiber material and the earthworm, and through repeated laboratory research and field tests, it is found that the three components have the advantages of good effect, low cost, no environmental pollution and wide application.

[0184] The present application provides a production method of chicory tea and a method for improving the chicoric acid content, which uses nitrogen-fixing bacteria, water-absorbing bamboo fiber material and earthworm, and provides a new perspective for improving the quality of chicory tea and promoting the growth of probiotic chicoric acid. The method provided by the present application can make the chicory growth composition and the earthworm better combined, and promote the growth of chicory. Moreover, the method provided by the present application can effectively improve the content of chicoric acid, and provides a new perspective for improving the quality of chicory tea and promoting the increase of chicoric acid content, and provides strong support for agricultural production.

[0185] The technical features of the above-described embodiments can be combined in any manner. To make the description concise, all possible combinations of the technical features in the above-described embodiments are not described, but as long as the combinations of the technical features do not contradict, they should be considered within the scope of the present disclosure.

[0186] The above-described embodiments only express several embodiments of the present application, and the description is more specific and detailed, but it should not be understood as limiting the scope of the patent. It should be noted that for ordinary skilled persons in the art, without departing from the concept of the present application, a number of modifications and improvements can be made, which are within the scope of the present application. Therefore, the protection scope of the present patent should be subject to the appended claims.

Claims

1. A method for producing chicory tea, characterized in that: The chicory growth composition is applied to the soil where chicory grows, and earthworm seedlings are placed in the soil. When chicory leaves grow out, they are harvested to make chicory tea. The application rate of the chicory growth composition is 79.62 g / m 2 ~477.71g / m 2 The amount of earthworm seedlings released is 1 / m 2 ~10 pieces / m 2 ; The chicory growth composition is made of the following raw materials in parts by weight: 1 to 15 parts of nitrogen-fixing bacteria and 5 to 60 parts of natural plant fiber materials with water absorption properties; The earthworm seedlings are Pheretima annuli ( Pheretima aspergillum )、Phea serrata ( Pheretima carnosa ) and Shanghai Dragon ( Shanghai Pheretima ) at least one of; The natural plant fiber material with water absorption performance is a water-absorbing bamboo fiber material; The preparation method of the water-absorbing bamboo fiber material comprises the following steps: Use bamboo stems of 250-350 days old as materials, clean them, and grind them into 75-85 mesh bamboo powder; use 60 The irradiation is carried out at a Co irradiation source and a irradiation dose of 15 kGy to 20 kGy at a temperature of 60° C. to 65° C. for 5 to 6 minutes to change the primary molecular structure of the bamboo fiber and obtain the water-absorbing bamboo fiber material.

2. The method for producing chicory tea according to claim 1, wherein: The nitrogen-fixing bacteria is rhizobium powder, and the concentration of live bacteria in the powder is 100 million CFU / g to 200 million CFU / g.

3. The method for producing chicory tea according to claim 1, wherein: Before the earthworm seedlings are placed in the soil, the following conditions must be maintained: the soil moisture content is maintained at 40% to 50%; the soil pH value is maintained at 6 to 8; and the soil temperature is maintained at 20° C. to 25° C.

4. The method for producing chicory tea according to claim 1, wherein: Before the earthworm seedlings are placed in the soil, they are firstly sprayed on the soil surface at a density of 1 earthworm / m 2 ~2 pieces / m 2 , dig trenches with a depth of 8cm~12cm, and then place earthworm seedlings into the trenches; the number of earthworm seedlings placed in each trench is 1~10.

5. The method for producing chicory tea according to claim 1, wherein: The production process of harvesting chicory leaves to make chicory tea includes the following steps: Spread out the fresh chicory leaves, the temperature is 15℃~26℃, the humidity is 65%~85%, grab the leaves continuously, they are soft and fluffy, and the moisture content is 55%~60%, the leaves are ready for greening and water removal; Entering the rolling stage, the temperature is 20℃~26℃, the humidity is 85%~90%, and the rolling is carried out for 40min~55min, and the strips are tightly tied; after taking out, ferment at a fermentation temperature of 25℃~27℃ and a humidity of 90%~100% for 2h~2.5h, dry, raise the temperature to 90℃~100℃, and continue for 10min~15min, so that the temperature of the fermented leaves reaches above 75℃, and the fermentation is terminated; dry at 85℃~95℃ to obtain chicory tea.

6. The chicory tea obtained by the method for producing chicory tea according to any one of claims 1 to 5, characterized in that: The chicoric acid content in the chicory tea is 0.30 mg / mL to 0.55 mg / mL.

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