Rhizoma pinellinae praeparata loquat leaf extract as well as preparation method and application thereof

Abstract

The invention belongs to the technical field of traditional Chinese medicine paste, and particularly relates to rhizoma pinellinae praeparata and loquat paste as well as a preparation method and application thereof. 10-15 parts of rhizoma pinellinae praeparata, 3-5 parts of folium eriobotryae, 18-20 parts of bulbus fritillariae cirrhosae, 5-7 parts of fructus momordicae, 25-30 parts of exocarpium citri rubrum, 5-6 parts of radix asteris, 10-12 parts of radix glycyrrhizae and 1-2 parts of menthol are specifically combined, release of beneficial functions of all the raw materials is promoted, and the synergistic interaction effect is achieved; in addition, on the basis, different treatment modes are adopted for different raw materials, and precipitation and retention of effective components in the raw materials are greatly promoted. Therefore, the rhizoma pinellinae praeparata loquat leaf extract provided by the invention has the characteristics of few types of raw materials and high nutritional value, and can be used for relieving cough.

Description

Technical Field

[0001] The invention belongs to the technical field of traditional Chinese medicine ointments, and particularly relates to a French pinellia and loquat ointment and a preparation method and application thereof. Background Art

[0002] French Pinellia belongs to the Araceae family. It is made from raw Pinellia through a specific processing process and can be used to treat symptoms such as damp phlegm, cold phlegm, and stomach qi reflux. Loquat leaves are the leaves of the Rosaceae plant loquat, also known as Baye and Lujuye. They have the effects of clearing the lungs and relieving coughs, harmonizing the stomach and promoting urination, and quenching thirst. Therefore, after mixing the two to make an ointment, it has a high application value.

[0003] There are reports on French Banxia Loquat Paste in the prior art, but the raw materials and preparation methods are mostly different. For example, in Chinese patent CN 116650589 A, French Banxia, ​​Momordica grosvenori, Chuanbeimu, Loquat Leaf, Platycodon grandiflorum, Bitter Almond, Tangerine Peel, Aster, Coltsfoot Flos, Fructus Trichosanthis, Polygala and Licorice are used as raw materials, and paste is made by ultrasound, boiling and other methods. Although there are many kinds of raw materials, the active ingredients of each raw material are not fully released, thus not only causing the waste of raw materials, but also reducing the antitussive effect of the paste. How to improve the efficacy of the paste on the basis of saving costs and reducing the types of raw materials is a technical problem to be solved in this field. Summary of the invention

[0004] The invention aims to provide a French pinellia and loquat paste and a preparation method and application thereof, which has the advantages of simple preparation method and good cough relieving effect.

[0005] The invention provides a French pinellia and loquat paste, which comprises the following raw materials in parts by weight: 10-15 parts of French pinellia, 3-5 parts of loquat leaves, 18-20 parts of Sichuan fritillaria, 5-7 parts of momordica grosvenori, 25-30 parts of tangerine peel, 5-6 parts of aster, 10-12 parts of liquorice and 1-2 parts of menthol.

[0006] The present invention provides a method for preparing the Banxia Loquat Paste described in the above technical solution, comprising the following steps:

[0007] The washed Pinellia ternata, Fritillaria cirrhosa, and loquat leaves are dried, crushed, and sieved in sequence to obtain Pinellia ternata powder, Fritillaria cirrhosa powder, and loquat leaf powder;

[0008] After soaking the monk fruit in a β-glucanase solution, freeze-drying, crushing and sieving are performed in sequence to obtain monk fruit powder;

[0009] Spraying Lactobacillus plantarum liquid on the surfaces of tangerine peel, aster and liquorice, and drying, crushing and sieving them in sequence to obtain tangerine peel powder, aster powder and liquorice powder;

[0010] Mixing the powder of Pinellia ternata, the powder of Fritillaria cirrhosa, the powder of loquat leaves, the powder of Momordica grosvenori, the powder of Citrus aurantium, the powder of Aster tataricus and the powder of Licorice to obtain a raw material powder;

[0011] The raw material powder is soaked in an ethanol solution with a volume concentration of 50%-60%, and after filtering, an extract and a mixed powder are obtained;

[0012] placing the mixed medicinal powder in pure water for ultrasonic extraction and decoction in sequence to obtain a decoction liquid;

[0013] The extract and the decoction are mixed and then juice is collected to obtain a concentrated juice;

[0014] Menthol and honey are added to the concentrated juice, and after collecting the paste and sterilizing, the French Pinellia Loquat Paste is obtained. Preferably, when the monk fruit is soaked in the β-glucanase solution, the soaking time is 35-45 minutes;

[0015] The mass concentration of the β-glucanase solution is 0.1%-0.2%.

[0016] Preferably, the freeze-drying temperature is -4 to 0°C; and the freeze-drying time is 15-25 minutes.

[0017] Preferably, the effective viable count of the Lactobacillus plantarum liquid is ≥ 0.2×10 7 cfu / mL.

[0018] Preferably, when the mixed powder is placed in pure water for ultrasonic extraction and decoction in sequence, the mass ratio of the material to the liquid is 1:25-30;

[0019] The ultrasonic extraction and decoction are performed 2-3 times; the decoction time is 3.5-4.5h / time;

[0020] The parameters of the ultrasonic extraction include: power of 320-350w, temperature of 42-45°C, and time of 1-2h / time.

[0021] Preferably, the French pinellia powder, cirrhosa powder, loquat leaf powder, momordica grosvenori powder, tangerine peel powder, aster powder and liquorice powder are respectively the undersizes obtained after passing through a 100-mesh sieve.

[0022] Preferably, the juice collection is performed by concentrating to a relative density of 1.12-1.15;

[0023] The cream juice is concentrated to a relative density of 1.38-1.42 to collect the cream.

[0024] Preferably, when the raw material powder is immersed in the ethanol solution, the immersion time is 68-72 hours; and the mass volume ratio of the raw material powder to the ethanol solution is 1 g: 15-20 mL.

[0025] The present invention also provides the use of the Banxia Pipa paste described in the above technical solution in the preparation of cough suppressant products.

[0026] Beneficial effects:

[0027] The present invention provides a French Banxia Loquat Paste, comprising the following raw materials in parts by weight: 10-15 parts of French Banxia, ​​3-5 parts of loquat leaves, 18-20 parts of Fritillaria cirrhosa, 5-7 parts of Momordica grosvenori, 25-30 parts of Citrus aurantium, 5-6 parts of Aster, 10-12 parts of Licorice and 1-2 parts of Menthol. The present invention promotes the release of functions between the raw materials and synergizes the synergy by specifically combining the above substances, which is beneficial to improving the antitussive effect of the French Banxia Loquat Paste.

[0028] The present invention also provides a method for preparing a French Banxia Loquat Paste, which is beneficial to the release and retention of active ingredients in each raw material by performing different treatments on different raw materials; soaking the monk fruit in a β-glucanase solution improves the purity of the effective active substances in the monk fruit; spraying the surface of tangerine peel, aster and licorice with plant lactobacillus liquid is beneficial to the improvement of the taste and flavor of subsequent products, and also increases its nutritional value. Therefore, the French Banxia Loquat Paste provided by the present invention has the characteristics of few raw material types and high nutritional value, and can be used for cough relief. DETAILED DESCRIPTION

[0029] In the present invention, unless otherwise specified, the methods and raw materials used are conventionally selected.

[0030] Arisaema erubescens (Wall.) Schott, a plant of the genus Pinellia in the family Araceae, has the effects of treating drying dampness and resolving phlegm, vertigo and palpitations caused by phlegm and fluid, dizziness caused by wind and phlegm, and headache caused by phlegm and syncope.

[0031] Loquat leaf (Folium Eriobotryae) is the leaf of the loquat plant of the Rosaceae family, also known as Ba leaf and Luju leaf. It has the effects of clearing the lungs and relieving cough, harmonizing the stomach and promoting diuresis, and quenching thirst.

[0032] Fritillaria cirrhosa D.Don is a perennial herb of the Liliaceae family and the genus Fritillaria. It has the effects of clearing away heat and moistening the lungs, resolving phlegm and relieving cough, and dispersing nodules and reducing swelling.

[0033] Momordica grosvenori has the effects of clearing heat and moistening the lungs, lubricating the intestines and relieving constipation, relieving sore throat and opening voice, moistening the intestines and relieving constipation, resolving phlegm and relieving cough, entering the lung meridian, and moistening the dry lungs.

[0034] Tangerine peel (Citrus maxima (Burm.) Merr. cv. Tomentosa) has the effects of drying dampness and resolving phlegm, regulating qi and promoting digestion.

[0035] Aster (Radix Asteris) has the effect of moistening the lungs and relieving cough.

[0036] Licorice (Glycyrrhiza uralensis Fisch.) has the effects of harmonizing the stomach, relieving urgency, moistening the lungs and detoxifying.

[0037] Menthol (Mentha canadaensis L.) has the effects of dispelling wind, clearing heat and detoxifying.

[0038] In order to further illustrate the present invention, the scheme provided by the present invention is described in detail below in conjunction with embodiments, but they should not be understood as limiting the protection scope of the present invention.

[0039] Example 1

[0040] A method for preparing Pinellia and Loquat Paste, comprising the following steps:

[0041] (1) The French Banxia Loquat Paste comprises the following raw materials by weight: 10 parts of French Banxia, ​​5 parts of loquat leaves, 18 parts of Chuanbei, 5 parts of Momordica grosvenori, 25 parts of Citrus aurantium, 5 parts of Aster, 10 parts of Licorice and 1 part of Menthol; the above raw materials are cleaned and set aside;

[0042] (2) Preparation method:

[0043] Washing, drying and crushing the pinellia tuber, fritillaria cirrhosa and loquat leaf respectively, and passing through a 100-mesh sieve to obtain pinellia tuber powder, fritillaria cirrhosa powder and loquat leaf powder;

[0044] Soaking the monk fruit in a β-glucanase solution (the mass concentration of the β-glucanase solution is 0.15%) for 40 minutes to obtain soaked monk fruit; drying the soaked monk fruit in a 0° C. environment for 20 minutes, and then crushing and passing through a 100-mesh sieve to obtain monk fruit powder;

[0045] The effective viable count was 0.2×10 7 cfu / mL of Lactobacillus plantarum bacterial liquid are evenly sprayed on the surfaces of tangerine peel, aster and licorice respectively, until the bacterial liquid does not drip on the surfaces of the medicinal materials, and then the tangerine peel, aster and licorice are dried and crushed at room temperature, and after passing through a 100-mesh sieve, tangerine peel powder, aster powder and licorice powder are obtained;

[0046] Mixing the powder of Pinellia ternata, the powder of Fritillaria cirrhosa, the powder of loquat leaves, the powder of Momordica grosvenori, the powder of Citrus aurantium, the powder of Aster tataricus and the powder of Licorice to obtain a raw material powder;

[0047] The raw material powder is immersed in an ethanol solution with a volume concentration of 50% for 72 hours (the mass volume ratio of the raw material powder to the ethanol solution is 1 g: 20 mL), and after filtering, an extract and a mixed powder are obtained;

[0048] The mixed powder was placed in pure water (the mass ratio of the mixed powder to water was 1:25) for ultrasonic extraction (ultrasonic extraction parameters: power 350w, temperature 42°C, time 1.5h); and then the mixed solution after ultrasonic extraction was decocted for 4h to obtain a first decoction and a first filter residue;

[0049] The first filter residue was placed in pure water (the mass ratio of the first filter residue to water was 1:25) for ultrasonic extraction (ultrasonic extraction parameters: power 350w, temperature 42°C, time 1.5h); and then the mixed solution after ultrasonic extraction was decocted for 4h to obtain a second decoction liquid and a second filter residue;

[0050] The second filter residue was placed in pure water (the mass ratio of the second filter residue to water was 1:25) for ultrasonic extraction (ultrasonic extraction parameters: power 350w, temperature 42°C, time 1.5h); and then the mixed solution after ultrasonic extraction was decocted for 4h to obtain a third decoction and a third filter residue;

[0051] The first decoction, the second decoction, the third decoction and the extract are combined and concentrated to a relative density of 1.14 to obtain a concentrated juice;

[0052] Menthol and honey in an amount 3 times the total mass of the raw materials of the French Pinellia and Loquat Paste are added to the concentrated juice to obtain a paste juice, and the paste juice is concentrated to a relative density of 1.4, and then the paste is collected and sterilized in sequence to obtain the French Pinellia and Loquat Paste.

[0053] Example 2

[0054] A method for preparing Pinellia and Loquat Paste, comprising the following steps:

[0055] (1) The French Banxia Loquat Paste comprises the following raw materials in parts by weight: 15 parts of French Banxia, ​​3 parts of loquat leaves, 20 parts of Chuanbei, 5 parts of Momordica grosvenori, 25 parts of Citrus aurantium, 5 parts of Aster, 10 parts of Licorice and 1 part of Menthol; the above raw materials are cleaned and set aside;

[0056] (2) Preparation method:

[0057] Washing, drying and crushing the pinellia tuber, fritillaria cirrhosa and loquat leaf respectively, and passing through a 100-mesh sieve to obtain pinellia tuber powder, fritillaria cirrhosa powder and loquat leaf powder;

[0058] Soaking the monk fruit in a β-glucanase solution (the mass concentration of the β-glucanase solution is 0.15%) for 40 minutes to obtain soaked monk fruit; drying the soaked monk fruit in a 0° C. environment for 20 minutes, and then crushing and passing through a 100-mesh sieve to obtain monk fruit powder;

[0059] The effective viable count was 0.2×10 7 cfu / mL of Lactobacillus plantarum bacterial liquid are evenly sprayed on the surfaces of tangerine peel, aster and licorice respectively, until the bacterial liquid does not drip on the surfaces of the medicinal materials, and then the tangerine peel, aster and licorice are dried and crushed at room temperature, and after passing through a 100-mesh sieve, tangerine peel powder, aster powder and licorice powder are obtained;

[0060] Mixing the powder of Pinellia ternata, the powder of Fritillaria cirrhosa, the powder of loquat leaves, the powder of Momordica grosvenori, the powder of Citrus aurantium, the powder of Aster tataricus and the powder of Licorice to obtain a raw material powder;

[0061] The raw material powder is immersed in an ethanol solution with a volume concentration of 50% for 72 hours (the mass volume ratio of the raw material powder to the ethanol solution is 1 g: 20 mL), and after filtering, an extract and a mixed powder are obtained;

[0062] The mixed powder was placed in pure water (the mass ratio of the mixed powder to water was 1:25) for ultrasonic extraction (ultrasonic extraction parameters: power 350w, temperature 45°C, time 2h); and then the mixed solution after ultrasonic extraction was decocted for 4h to obtain a first decoction and a first filter residue;

[0063] The first filter residue was placed in pure water (the mass ratio of the first filter residue to water was 1:25) for ultrasonic extraction (ultrasonic extraction parameters: power 350w, temperature 45°C, time 2h); and then the mixed solution after ultrasonic extraction was decocted for 4h to obtain a second decoction liquid and a second filter residue;

[0064] The second filter residue is placed in pure water (the mass ratio of the second filter residue to water is 1:25) for ultrasonic extraction (ultrasonic extraction parameters: power 350w, temperature 45°C, time 2h); then the mixed solution after ultrasonic extraction is decocted for 4h to obtain a third decoction and a third filter residue;

[0065] The first decoction, the second decoction, the third decoction and the extract are combined and concentrated to a relative density of 1.14 to obtain a concentrated juice;

[0066] Menthol and honey in an amount 3 times the total mass of the raw materials of the French Pinellia and Loquat Paste are added to the concentrated juice to obtain a paste juice, and the paste juice is concentrated to a relative density of 1.4, and then the paste is collected and sterilized in sequence to obtain the French Pinellia and Loquat Paste.

[0067] Comparative Example 1

[0068] (1) The French Banxia Loquat Paste comprises the following raw materials in parts by weight: 10 parts of French Banxia, ​​5 parts of loquat, 18 parts of Chuanbei, 5 parts of Momordica grosvenori, 25 parts of Citrus aurantium, 5 parts of Aster, 10 parts of Licorice and 1 part of Menthol; the above raw materials are cleaned and set aside;

[0069] (2) Preparation method:

[0070] Washing, drying and crushing the pinellia tuber, fritillaria cirrhosa and loquat fruit respectively, and then passing through a 100-mesh sieve to obtain pinellia tuber powder, fritillaria cirrhosa powder and loquat fruit powder;

[0071] Soaking the monk fruit in a β-glucanase solution (the mass concentration of the β-glucanase solution is 0.15%) for 40 minutes to obtain soaked monk fruit; drying the soaked monk fruit in a 0° C. environment for 20 minutes, and then crushing and passing through a 100-mesh sieve to obtain monk fruit powder;

[0072] The effective viable count was 0.2×10 7 cfu / mL of Lactobacillus plantarum bacterial liquid are evenly sprayed on the surfaces of tangerine peel, aster and licorice respectively, until the bacterial liquid does not drip on the surfaces of the medicinal materials, and then the tangerine peel, aster and licorice are dried and crushed at room temperature, and after passing through a 100-mesh sieve, tangerine peel powder, aster powder and licorice powder are obtained;

[0073] Mixing the powder of Pinellia ternata, the powder of Fritillaria cirrhosa, the powder of loquat, the powder of Momordica grosvenori, the powder of Citrus aurantium, the powder of Aster tataricus and the powder of Licorice to obtain a raw material powder;

[0074] The raw material powder is immersed in an ethanol solution with a volume concentration of 50% for 72 hours (the mass volume ratio of the raw material powder to the ethanol solution is 1 g: 20 mL), and after filtering, an extract and a mixed powder are obtained;

[0075] The mixed powder was placed in pure water (the mass ratio of the mixed powder to water was 1:25) for ultrasonic extraction (ultrasonic extraction parameters: power 350w, temperature 42°C, time 1.5h); and then the mixed solution after ultrasonic extraction was decocted for 4h to obtain a first decoction and a first filter residue;

[0076] The first filter residue was placed in pure water (the mass ratio of the first filter residue to water was 1:25) for ultrasonic extraction (ultrasonic extraction parameters: power 350w, temperature 42°C, time 1.5h); and then the mixed solution after ultrasonic extraction was decocted for 4h to obtain a second decoction liquid and a second filter residue;

[0077] The second filter residue was placed in pure water (the mass ratio of the second filter residue to water was 1:25) for ultrasonic extraction (ultrasonic extraction parameters: power 350w, temperature 42°C, time 1.5h); and then the mixed solution after ultrasonic extraction was decocted for 4h to obtain a third decoction and a third filter residue;

[0078] The first decoction, the second decoction, the third decoction and the extract are combined and concentrated to a relative density of 1.14 to obtain a concentrated juice;

[0079] Menthol and honey in an amount 3 times the total mass of the raw materials of the French Pinellia and Loquat Paste are added to the concentrated juice to obtain a paste juice, and the paste juice is concentrated to a relative density of 1.4, and then the paste is collected and sterilized in sequence to obtain the French Pinellia and Loquat Paste.

[0080] Comparative Example 2

[0081] (1) The French Banxia Loquat Paste comprises the following raw materials by weight: 10 parts of French Banxia, ​​5 parts of loquat leaves, 18 parts of Chuanbei, 5 parts of Momordica grosvenori, 25 parts of Citrus aurantium, 5 parts of Aster, 10 parts of Licorice and 1 part of Menthol; the above raw materials are cleaned and set aside;

[0082] (2) Preparation method:

[0083] Wash, dry and crush the Chinese Pinellia tuber, Fritillaria cirrhosa, loquat leaf, dried tangerine peel, aster and liquorice respectively, and pass through a 100-mesh sieve to obtain Chinese Pinellia tuber powder, Fritillaria cirrhosa powder, loquat leaf powder, dried tangerine peel powder, aster powder and liquorice powder;

[0084] Soaking the monk fruit in a β-glucanase solution (the mass concentration of the β-glucanase solution is 0.15%) for 40 minutes to obtain soaked monk fruit; drying the soaked monk fruit in a 0° C. environment for 20 minutes, and then crushing and passing through a 100-mesh sieve to obtain monk fruit powder;

[0085] Mixing the powder of Pinellia ternata, the powder of Fritillaria cirrhosa, the powder of loquat leaves, the powder of Momordica grosvenori, the powder of Citrus aurantium, the powder of Aster tataricus and the powder of Licorice to obtain a raw material powder;

[0086] The raw material powder is immersed in an ethanol solution with a volume concentration of 50% for 72 hours (the mass volume ratio of the raw material powder to the ethanol solution is 1 g: 20 mL), and after filtering, an extract and a mixed powder are obtained;

[0087] The mixed powder was placed in pure water (the mass ratio of the mixed powder to water was 1:25) for ultrasonic extraction (ultrasonic extraction parameters: power 350w, temperature 42°C, time 1.5h); and then the mixed solution after ultrasonic extraction was decocted for 4h to obtain a first decoction and a first filter residue;

[0088] The first filter residue was placed in pure water (the mass ratio of the first filter residue to water was 1:25) for ultrasonic extraction (ultrasonic extraction parameters: power 350w, temperature 42°C, time 1.5h); and then the mixed solution after ultrasonic extraction was decocted for 4h to obtain a second decoction liquid and a second filter residue;

[0089] The second filter residue was placed in pure water (the mass ratio of the second filter residue to water was 1:25) for ultrasonic extraction (ultrasonic extraction parameters: power 350w, temperature 42°C, time 1.5h); and then the mixed solution after ultrasonic extraction was decocted for 4h to obtain a third decoction and a third filter residue;

[0090] The first decoction, the second decoction, the third decoction and the extract are combined and concentrated to a relative density of 1.14 to obtain a concentrated juice;

[0091] Menthol and honey in an amount 3 times the total mass of the raw materials of the French Pinellia and Loquat Paste are added to the concentrated juice to obtain a paste juice, and the paste juice is concentrated to a relative density of 1.4, and then the paste is collected and sterilized in sequence to obtain the French Pinellia and Loquat Paste.

[0092] Comparative Example 3

[0093] A method for preparing Pinellia and Loquat Paste, comprising the following steps:

[0094] (1) The French Banxia Loquat Paste comprises the following raw materials in parts by weight: 20 parts of French Banxia, ​​50 parts of loquat leaves, 18 parts of Chuanbei, 7 parts of Momordica grosvenori, 25 parts of Citrus aurantium, 5 parts of Aster, 10 parts of Licorice, 3 parts of Citrus aurantium, 4 parts of Citrus aurantium, 6 parts of Radish Seed and 1 part of Menthol. The above raw materials are disinfected with 75% alcohol by volume, washed, dried and set aside;

[0095] (2) Preparation method:

[0096] Wash, dry and crush the pinellia tuber, fritillaria cirrhosa, loquat leaf, tangerine peel, fructus aurantii and radish seed respectively, and pass through a 100-mesh sieve to obtain pinellia tuber powder, fritillaria cirrhosa powder, loquat leaf powder, tangerine peel powder, fructus aurantii powder and radish seed powder;

[0097] Soaking the monk fruit in a β-glucanase solution (the mass concentration of the β-glucanase solution is 0.15%) for 40 minutes to obtain soaked monk fruit; drying the soaked monk fruit in a 0° C. environment for 20 minutes, and then crushing and passing through a 100-mesh sieve to obtain monk fruit powder;

[0098] The effective viable count was 0.2×10 7cfu / mL of Lactobacillus plantarum bacterial liquid are evenly sprayed on the surfaces of tangerine peel, aster and licorice respectively, until the bacterial liquid does not drip on the surfaces of the medicinal materials, and then the tangerine peel, aster and licorice are dried and crushed at room temperature, and after passing through a 100-mesh sieve, tangerine peel powder, aster powder and licorice powder are obtained;

[0099] Mixing the powder of Pinellia ternata, the powder of Fritillaria cirrhosa, the powder of loquat leaf, the powder of Citrus aurantium, the powder of Fructus Aurantii Immaturus, the powder of Semen Raphani, the powder of Momordica grosvenori, the powder of Citrus aurantium, the powder of Aster tataricus and the powder of Licorice to obtain a raw material powder;

[0100] The raw material powder is immersed in an ethanol solution with a volume concentration of 50% for 72 hours (the mass volume ratio of the raw material powder to the ethanol solution is 1 g: 20 mL), and after filtering, an extract and a mixed powder are obtained;

[0101] The mixed powder was placed in pure water (the mass ratio of the mixed powder to water was 1:25) for ultrasonic extraction (ultrasonic extraction parameters: power 350w, temperature 42°C, time 1.5h); and then the mixed solution after ultrasonic extraction was decocted for 4h to obtain a first decoction and a first filter residue;

[0102] The first filter residue was placed in pure water (the mass ratio of the first filter residue to water was 1:25) for ultrasonic extraction (ultrasonic extraction parameters: power 350w, temperature 42°C, time 1.5h); and then the mixed solution after ultrasonic extraction was decocted for 4h to obtain a second decoction liquid and a second filter residue;

[0103] The second filter residue was placed in pure water (the mass ratio of the second filter residue to water was 1:25) for ultrasonic extraction (ultrasonic extraction parameters: power 350w, temperature 42°C, time 1.5h); and then the mixed solution after ultrasonic extraction was decocted for 4h to obtain a third decoction and a third filter residue;

[0104] The first decoction, the second decoction, the third decoction and the extract are combined and concentrated to a relative density of 1.14 to obtain a concentrated juice;

[0105] Menthol and honey in an amount 3 times the total mass of the raw materials of the French Pinellia and Loquat Paste are added to the concentrated juice to obtain a paste juice, and the paste juice is concentrated to a relative density of 1.4, and then the paste is collected and sterilized in sequence to obtain the French Pinellia and Loquat Paste.

[0106] Comparative Example 4

[0107] (1) The French Banxia Loquat Paste comprises the following raw materials by weight: 10 parts of French Banxia, ​​5 parts of loquat leaves, 18 parts of Chuanbei, 5 parts of Momordica grosvenori, 25 parts of Citrus aurantium, 5 parts of Aster, 10 parts of Licorice and 1 part of Menthol; the above raw materials are cleaned and set aside;

[0108] (2) Preparation method:

[0109] Washing, drying and crushing the pinellia tuber, fritillaria cirrhosa and loquat leaf respectively, and passing through a 100-mesh sieve to obtain pinellia tuber powder, fritillaria cirrhosa powder and loquat leaf powder;

[0110] Soaking the monk fruit in a β-glucanase solution (the mass concentration of the β-glucanase solution is 0.15%) for 40 minutes to obtain soaked monk fruit; drying the soaked monk fruit in a 0° C. environment for 20 minutes, and then crushing and passing through a 100-mesh sieve to obtain monk fruit powder;

[0111] The effective viable count was 0.2×10 7 cfu / mL of Bacillus subtilis bacterial solution are evenly sprayed on the surface of tangerine peel, aster and licorice, respectively, until the bacterial solution does not drip on the surface of the medicinal materials, and then tangerine peel, aster and licorice are dried and crushed at room temperature, and passed through a 100-mesh sieve to obtain tangerine peel powder, aster powder and licorice powder;

[0112] Mixing the powder of Pinellia ternata, the powder of Fritillaria cirrhosa, the powder of loquat leaves, the powder of Momordica grosvenori, the powder of Citrus aurantium, the powder of Aster tataricus and the powder of Licorice to obtain a raw material powder;

[0113] The raw material powder is immersed in an ethanol solution with a volume concentration of 50% for 72 hours (the mass volume ratio of the raw material powder to the ethanol solution is 1 g: 20 mL), and after filtering, an extract and a mixed powder are obtained;

[0114] The mixed powder was placed in pure water (the mass ratio of the mixed powder to water was 1:25) for ultrasonic extraction (ultrasonic extraction parameters: power 350w, temperature 42°C, time 1.5h); and then the mixed solution after ultrasonic extraction was decocted for 4h to obtain a first decoction and a first filter residue;

[0115] The first filter residue was placed in pure water (the mass ratio of the first filter residue to water was 1:25) for ultrasonic extraction (ultrasonic extraction parameters: power 350w, temperature 42°C, time 1.5h); and then the mixed solution after ultrasonic extraction was decocted for 4h to obtain a second decoction liquid and a second filter residue;

[0116] The second filter residue was placed in pure water (the mass ratio of the second filter residue to water was 1:25) for ultrasonic extraction (ultrasonic extraction parameters: power 350w, temperature 42°C, time 1.5h); and then the mixed solution after ultrasonic extraction was decocted for 4h to obtain a third decoction and a third filter residue;

[0117] The first decoction, the second decoction, the third decoction and the extract are combined and concentrated to a relative density of 1.14 to obtain a concentrated juice;

[0118] Menthol and honey in an amount 3 times the total mass of the raw materials of the French Pinellia and Loquat Paste are added to the concentrated juice to obtain a paste juice, and the paste juice is concentrated to a relative density of 1.4, and then the paste is collected and sterilized in sequence to obtain the French Pinellia and Loquat Paste.

[0119] Comparative Example 5

[0120] (1) The French Banxia Loquat Paste comprises the following raw materials by weight: 10 parts of French Banxia, ​​5 parts of loquat leaves, 18 parts of Chuanbei, 5 parts of Momordica grosvenori, 25 parts of Citrus aurantium, 5 parts of Aster, 10 parts of Licorice and 1 part of Menthol; the above raw materials are cleaned and set aside;

[0121] (2) Preparation method:

[0122] Washing, drying and crushing the pinellia tuber, fritillaria cirrhosa and loquat leaf respectively, and passing through a 100-mesh sieve to obtain pinellia tuber powder, fritillaria cirrhosa powder and loquat leaf powder;

[0123] The monk fruit is soaked in a β-glucanase solution (the mass concentration of the β-glucanase solution is 0.30%) for 40 minutes to obtain the soaked monk fruit; the soaked monk fruit is dried in an environment of 0° C. for 20 minutes, and then crushed and passed through a 100-mesh sieve to obtain the monk fruit powder;

[0124] The effective viable count was 0.1×10 7 cfu / mL of Lactobacillus plantarum bacterial liquid are evenly sprayed on the surfaces of tangerine peel, aster and licorice respectively, until the bacterial liquid does not drip on the surfaces of the medicinal materials, and then the tangerine peel, aster and licorice are dried and crushed at room temperature, and after passing through a 100-mesh sieve, tangerine peel powder, aster powder and licorice powder are obtained;

[0125] Mixing the powder of Pinellia ternata, the powder of Fritillaria cirrhosa, the powder of loquat leaves, the powder of Momordica grosvenori, the powder of Citrus aurantium, the powder of Aster tataricus and the powder of Licorice to obtain a raw material powder;

[0126] The raw material powder is immersed in an ethanol solution with a volume concentration of 50% for 72 hours (the mass volume ratio of the raw material powder to the ethanol solution is 1 g: 20 mL), and after filtering, an extract and a mixed powder are obtained;

[0127] The mixed powder was placed in pure water (the mass ratio of the mixed powder to water was 1:25) for ultrasonic extraction (ultrasonic extraction parameters: power 350w, temperature 42°C, time 1.5h); and then the mixed solution after ultrasonic extraction was decocted for 4h to obtain a first decoction and a first filter residue;

[0128] The first filter residue was placed in pure water (the mass ratio of the first filter residue to water was 1:25) for ultrasonic extraction (ultrasonic extraction parameters: power 350w, temperature 42°C, time 1.5h); and then the mixed solution after ultrasonic extraction was decocted for 4h to obtain a second decoction liquid and a second filter residue;

[0129] The second filter residue was placed in pure water (the mass ratio of the second filter residue to water was 1:25) for ultrasonic extraction (ultrasonic extraction parameters: power 350w, temperature 42°C, time 1.5h); and then the mixed solution after ultrasonic extraction was decocted for 4h to obtain a third decoction and a third filter residue;

[0130] The first decoction, the second decoction, the third decoction and the extract are combined and concentrated to a relative density of 1.14 to obtain a concentrated juice;

[0131] Menthol and honey in an amount 3 times the total mass of the raw materials of the French Pinellia and Loquat Paste are added to the concentrated juice to obtain a paste juice, and the paste juice is concentrated to a relative density of 1.4, and then the paste is collected and sterilized in sequence to obtain the French Pinellia and Loquat Paste.

[0132] Comparative Example 6

[0133] (1) The French Banxia Loquat Paste comprises the following raw materials by weight: 10 parts of French Banxia, ​​5 parts of loquat leaves, 18 parts of Chuanbei, 5 parts of Momordica grosvenori, 25 parts of Citrus aurantium, 5 parts of Aster, 10 parts of Licorice and 1 part of Menthol; the above raw materials are cleaned and set aside;

[0134] (2) Preparation method:

[0135] Washing, drying and crushing the pinellia tuber, fritillaria cirrhosa, loquat leaf and momordica grosvenori respectively, and passing through a 100-mesh sieve to obtain pinellia tuber powder, fritillaria cirrhosa powder, loquat leaf powder and momordica grosvenori powder;

[0136] The effective viable count was 0.2×10 7 cfu / mL of Lactobacillus plantarum bacterial liquid are evenly sprayed on the surfaces of tangerine peel, aster and licorice respectively, until the bacterial liquid does not drip on the surfaces of the medicinal materials, and then the tangerine peel, aster and licorice are dried and crushed at room temperature, and after passing through a 100-mesh sieve, tangerine peel powder, aster powder and licorice powder are obtained;

[0137] Mixing the powder of Pinellia ternata, the powder of Fritillaria cirrhosa, the powder of loquat leaves, the powder of Momordica grosvenori, the powder of Citrus aurantium, the powder of Aster tataricus and the powder of Licorice to obtain a raw material powder;

[0138] The raw material powder is immersed in an ethanol solution with a volume concentration of 50% for 72 hours (the mass volume ratio of the raw material powder to the ethanol solution is 1 g: 20 mL), and after filtering, an extract and a mixed powder are obtained;

[0139] The mixed powder was placed in pure water (the mass ratio of the mixed powder to water was 1:25) for ultrasonic extraction (ultrasonic extraction parameters: power 350w, temperature 42°C, time 1.5h); and then the mixed solution after ultrasonic extraction was decocted for 4h to obtain a first decoction and a first filter residue;

[0140] The first filter residue was placed in pure water (the mass ratio of the first filter residue to water was 1:25) for ultrasonic extraction (ultrasonic extraction parameters: power 350w, temperature 42°C, time 1.5h); and then the mixed solution after ultrasonic extraction was decocted for 4h to obtain a second decoction liquid and a second filter residue;

[0141] The second filter residue was placed in pure water (the mass ratio of the second filter residue to water was 1:25) for ultrasonic extraction (ultrasonic extraction parameters: power 350w, temperature 42°C, time 1.5h); and then the mixed solution after ultrasonic extraction was decocted for 4h to obtain a third decoction and a third filter residue;

[0142] The first decoction, the second decoction, the third decoction and the extract are combined and concentrated to a relative density of 1.14 to obtain a concentrated juice;

[0143] Menthol and honey in an amount 3 times the total mass of the raw materials of the French Pinellia and Loquat Paste are added to the concentrated juice to obtain a paste juice, and the paste juice is concentrated to a relative density of 1.4, and then the paste is collected and sterilized in sequence to obtain the French Pinellia and Loquat Paste.

[0144] Comparative Example 7

[0145] A method for preparing Pinellia and Loquat Paste, comprising the following steps:

[0146] (1) The French Banxia Loquat Paste comprises the following raw materials by weight: 10 parts of French Banxia, ​​5 parts of loquat leaves, 18 parts of Chuanbei, 5 parts of Momordica grosvenori, 25 parts of Citrus aurantium, 5 parts of Aster, 10 parts of Licorice and 1 part of Menthol; the above raw materials are cleaned and set aside;

[0147] (2) Preparation method:

[0148] Soaking the French pinellia, loquat leaves, Fritillaria cirrhosa, Momordica grosvenori, tangerine peel, Aster tataricus and liquorice in water for 10 hours (the water completely covers each raw material) to obtain soaked raw materials;

[0149] The soaked raw material was mixed with water in a mass ratio of 1:15 and then subjected to ultrasonic extraction (ultrasonic extraction parameters: power 350w, temperature 42°C, time 1.5h), and after filtration, the filter residue and ultrasonic extraction solution were obtained for use;

[0150] The filtrate was mixed with water in a mass ratio of 1:15 and decocted twice, each time for 4 hours, and the filtrates obtained after each decoction were mixed to obtain a medicinal juice;

[0151] The ultrasonic extract and the medicinal juice are mixed and concentrated to a relative density of 1.14 to obtain a concentrated juice;

[0152] Menthol and honey in an amount 3 times the total mass of the raw materials of the French Pinellia and Loquat Paste are added to the concentrated juice to obtain a paste juice, and the paste juice is concentrated to a relative density of 1.4, and then the paste is collected and sterilized in sequence to obtain the French Pinellia and Loquat Paste.

[0153] Comparative Example 8

[0154] A method for preparing Pinellia and Loquat Paste, comprising the following steps:

[0155] (1) The French Banxia Loquat Paste comprises the following raw materials by weight: 10 parts of French Banxia, ​​5 parts of loquat leaves, 18 parts of Chuanbei, 5 parts of Momordica grosvenori, 25 parts of Citrus aurantium, 5 parts of Aster, 10 parts of Licorice and 1 part of Menthol; the above raw materials are cleaned and set aside;

[0156] (2) Preparation method:

[0157] Washing, drying and crushing the pinellia tuber, fritillaria cirrhosa and loquat leaf respectively, and passing through a 100-mesh sieve to obtain pinellia tuber powder, fritillaria cirrhosa powder and loquat leaf powder;

[0158] The monk fruit is soaked in an amylase solution (the mass concentration of the amylase solution is 0.15%) for 40 minutes to obtain the soaked monk fruit; the soaked monk fruit is dried in an environment of 0° C. for 20 minutes, and then crushed and passed through a 100-mesh sieve to obtain the monk fruit powder;

[0159] The effective viable count was 0.2×10 7 cfu / mL of Lactobacillus plantarum bacterial liquid are evenly sprayed on the surfaces of tangerine peel, aster and licorice respectively, until the bacterial liquid does not drip on the surfaces of the medicinal materials, and then the tangerine peel, aster and licorice are dried and crushed at room temperature, and after passing through a 100-mesh sieve, tangerine peel powder, aster powder and licorice powder are obtained;

[0160] Mixing the powder of Pinellia ternata, the powder of Fritillaria cirrhosa, the powder of loquat leaves, the powder of Momordica grosvenori, the powder of Citrus aurantium, the powder of Aster tataricus and the powder of Licorice to obtain a raw material powder;

[0161] The raw material powder is immersed in an ethanol solution with a volume concentration of 80% for 72 hours (the mass volume ratio of the raw material powder to the ethanol solution is 1 g: 20 mL), and after filtering, an extract and a mixed powder are obtained;

[0162] The mixed powder was placed in pure water (the mass ratio of the mixed powder to water was 1:25) for ultrasonic extraction (ultrasonic extraction parameters: power 350w, temperature 42°C, time 1.5h); and then the mixed solution after ultrasonic extraction was decocted for 4h to obtain a first decoction and a first filter residue;

[0163] The first filter residue was placed in pure water (the mass ratio of the first filter residue to water was 1:25) for ultrasonic extraction (ultrasonic extraction parameters: power 350w, temperature 42°C, time 1.5h); and then the mixed solution after ultrasonic extraction was decocted for 4h to obtain a second decoction liquid and a second filter residue;

[0164] The second filter residue was placed in pure water (the mass ratio of the second filter residue to water was 1:25) for ultrasonic extraction (ultrasonic extraction parameters: power 350w, temperature 42°C, time 1.5h); and then the mixed solution after ultrasonic extraction was decocted for 4h to obtain a third decoction and a third filter residue;

[0165] The first decoction, the second decoction, the third decoction and the extract are combined and concentrated to a relative density of 1.20 to obtain a concentrated juice;

[0166] Menthol and honey in an amount 3 times the total mass of the raw materials of the French Pinellia and Loquat Paste are added to the concentrated juice to obtain a paste juice, and the paste juice is concentrated to a relative density of 1.50, and then the paste is collected and sterilized in sequence to obtain the French Pinellia and Loquat Paste.

[0167] Comparative Example 9

[0168] Regularly purchase compound loquat paste.

[0169] Application Example 1

[0170] Mice weighing 19-21 g and in good health were selected and raised in the same environment for 2 days; the mice were evenly divided into 12 groups, with 10 mice in each group, for subsequent verification;

[0171] Take 1 g of the French Pinellia Loquat Paste (or Compound Loquat Paste) in Examples 1-2 and Comparative Examples 1-9, respectively, dilute with 10 mL of pure water to obtain 0.1 g / mL of the test substance; gavage the above test substances to mice in different treatment groups, each mouse gavage once a day, 0.5 mL each time, and mice gavaged with the same mass of normal saline were used as blank controls;

[0172] The mice were treated with ammonia-induced coughing method 1 hour after the last administration of the drug for 3 consecutive days, that is, a cotton ball was placed in an inverted beaker and concentrated ammonia was injected. The mice were placed in the beaker and taken out after 5 seconds. The average number of coughs of the mice within 3 minutes (i.e., the total number of coughs of 10 mice in the same group within 3 minutes divided by 10) and the average time to the first onset of coughing (i.e., the sum of the time to the first cough of 10 mice in the same group divided by 10) were observed and recorded. The results are shown in Table 1.

[0173] Table 1 Effects of different treatments on coughing in mice

[0174]

[0175]

[0176] Combined with the data in Table 1, it can be seen that compared with the blank control, the scheme in Example 1-2 can significantly reduce the number of coughs in mice and prolong the time to the first onset of coughing; compared with Comparative Examples 1-9, the average number of coughs in mice in Example 1-2 is only 2.5-2.8, while the minimum number of coughs in the comparative example is 3.1 times. It can be seen that after adjusting the raw material components, preparation method or preparation parameters of the Banxia Loquat Paste, the release of the effective functions of each raw material in the paste is reduced, which is not conducive to the paste to exert its cough-relieving effect.

[0177] In summary, the present invention promotes the release of the beneficial functions of each raw material by specifically combining 10-15 parts of French pinellia, 3-5 parts of loquat leaves, 18-20 parts of Fritillaria cirrhosa, 5-7 parts of Momordica grosvenori, 25-30 parts of tangerine peel, 5-6 parts of aster, 10-12 parts of licorice and 1-2 parts of menthol; in addition, on this basis, different raw materials are treated in different ways, which greatly promotes the precipitation and retention of effective ingredients in each raw material, thereby achieving the synergistic effect of each raw material. Therefore, the French pinellia loquat paste provided by the present invention has the characteristics of few raw material types and high nutritional value, and the corresponding product can be used for cough relief.

[0178] Although the above embodiment describes the present invention in detail, it is only a part of the embodiments of the present invention, not all of the embodiments. People can also obtain other embodiments based on this embodiment without creativity, and these embodiments all fall within the protection scope of the present invention.

Claims

1. A French Pinellia and Loquat Paste, characterized in that: The invention comprises the following raw materials in parts by weight: 10-15 parts of French pinellia, 3-5 parts of loquat leaves, 18-20 parts of Sichuan fritillaria, 5-7 parts of momordica grosvenori, 25-30 parts of tangerine peel, 5-6 parts of aster, 10-12 parts of liquorice and 1-2 parts of menthol.

2. The method for preparing the Banxia Pipa paste according to claim 1, characterized in that: The steps include: The washed Pinellia ternata, Fritillaria cirrhosa and loquat leaves are dried, crushed and sieved in sequence to obtain Pinellia ternata powder, Fritillaria cirrhosa powder and loquat leaf powder; After soaking the monk fruit in a β-glucanase solution, freeze-drying, crushing and sieving are performed in sequence to obtain monk fruit powder; Spraying Lactobacillus plantarum liquid on the surfaces of tangerine peel, aster and liquorice, and drying, crushing and sieving them in sequence to obtain tangerine peel powder, aster powder and liquorice powder; Mixing the powder of Pinellia ternata, the powder of Fritillaria cirrhosa, the powder of loquat leaves, the powder of Momordica grosvenori, the powder of Citrus aurantium, the powder of Aster tataricus and the powder of Licorice to obtain a raw material powder; The raw material powder is soaked in an ethanol solution with a volume concentration of 50%-60%, and after filtering, an extract and a mixed powder are obtained; placing the mixed medicinal powder in pure water for ultrasonic extraction and decoction in sequence to obtain a decoction liquid; The extract and the decoction are mixed and then juice is collected to obtain a concentrated juice; Menthol and honey are added to the concentrated juice, and after collecting the paste and sterilizing, the French Pinellia and Loquat paste is obtained.

3. The preparation method according to claim 2, characterized in that: When the monk fruit is soaked in the β-glucanase solution, the soaking time is 35-45 minutes; The mass concentration of the β-glucanase solution is 0.1%-0.2%.

4. The preparation method according to claim 2, characterized in that: The freeze-drying temperature is -4 to 0°C; the freeze-drying time is 15-25 minutes.

5. The preparation method according to claim 2, characterized in that: The effective viable bacteria count of the Lactobacillus plantarum liquid is ≥ 0.2×10 7 cfu / mL.

6. The preparation method according to claim 2, characterized in that: When the mixed powder is placed in pure water for ultrasonic extraction and decoction in sequence, the mass ratio of the material to the liquid is 1:25-30; The ultrasonic extraction and decoction are performed 2-3 times; the decoction time is 3.5-4.5h / time; The parameters of the ultrasonic extraction include: power of 320-350w, temperature of 42-45°C, and time of 1-2h / time.

7. The preparation method according to claim 2, characterized in that: The French pinellia powder, Sichuan fritillaria powder, loquat leaf powder, momordica grosvenori powder, tangerine peel powder, aster powder and liquorice powder are respectively the sieve-downs obtained after passing through a 100-mesh sieve.

8. The preparation method according to claim 2, characterized in that: Concentrating to a relative density of 1.12-1.15 to collect the juice; The cream juice is concentrated to a relative density of 1.38-1.42 to collect the cream.

9. The preparation method according to claim 2, characterized in that: When the raw material powder is immersed in the ethanol solution, the immersion time is 68-72 hours; the mass volume ratio of the raw material powder to the ethanol solution is 1g:15-20mL.

10. Use of the Banxia Pipa paste according to claim 1 in the preparation of cough suppressant products.

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