Desert area drilling water-based solid waste biological treatment resource utilization method

Abstract

The invention discloses a desert area water-based drilling solid waste biological treatment resource utilization method, and belongs to the technical field of petroleum and natural gas exploration drilling pollution abatement, and the desert area water-based drilling solid waste biological treatment resource utilization method comprises the following steps: preparing a desert area water-based drilling solid waste biological treatment resource preparation; uniformly mixing the biological agent and the desert area water-based drilling solid waste according to a weight ratio of 0.1-1.0% wt to obtain a first mixture; adding a nutritional agent which accounts for 1-5 wt% of the weight of the first mixture, and uniformly mixing to obtain a second mixture; the second mixture is transferred to a treatment place, the water content is controlled to be 22%-30%, treatment is conducted for 90-120 days at the temperature of 10-45 DEG C, and green planting soil is obtained; wherein the biological agent is a mixture of a bacterial inoculant and a fungus solid inoculant with the same mass. The method can adapt to sandy water-based drilling solid waste treatment, is wide in treatment temperature range, and is suitable for a low-temperature field environment in a desert area.

Description

Technical Field

[0001] The invention belongs to the technical field of pollution control in oil and gas exploration drilling, and specifically relates to a method for resource utilization of water-based drilling solid waste biological treatment in desert areas. Background Art

[0002] Water-based drilling solid waste is an inevitable product of conventional drilling operations in oil and gas exploration. The amount produced in desert areas (Changqing and Xinjiang) is generally 1,300m3 / well. Since drilling in desert areas is relatively scattered, the method of centralized transportation and treatment has high treatment costs and environmental risks. On the other hand, the structural system of water-based solid waste in desert areas is sandy, with a good nutrient base such as nitrogen, phosphorus, potassium and organic matter, and has the potential to be converted into soil for resource utilization through biological treatment.

[0003] The two patents with publication numbers CN112715319B (polymer system water-based drilling solid waste bio-enhanced treatment process and greening planting soil) and CN112775157B (sulfonation system water-based drilling solid waste bio-enhanced treatment process and greening planting soil) both disclose the use of microbial technology to treat water-based drilling solid waste and transform it into greening planting soil. However, in actual application, the above methods treat loamy water-based drilling solid waste in Sichuan and Chongqing. CN112775157B requires high-temperature treatment stages and equipment, and the applicable temperature range for treatment by CN112715319B is 15 to 35°C, which is a relatively narrow range. In view of the above problems, it is necessary to provide a method for resource utilization of water-based drilling solid waste that can adapt to sandy water-based drilling solid waste in desert areas, has a wide treatment temperature range, and is suitable for medium and low temperatures. Summary of the invention

[0004] The purpose of the present invention is to solve the above-mentioned deficiencies in the prior art. The purpose of the present invention is to provide a method for biological treatment and resource utilization of water-based drilling solid waste in desert areas that is suitable for medium and low temperatures.

[0005] The objective of the present invention is achieved through the following technical solutions: A method for biological treatment and resource utilization of water-based drilling solid waste in desert areas, comprising the following steps: Preparation of biological agents for biological treatment and resource utilization of water-based drilling solid waste in desert areas; The biological agent and the water-based drilling solid waste in the desert area are mixed uniformly at a weight ratio of 0.1 to 1.0% wt to obtain a first mixture; Adding 1 to 5% wt of the nutrient agent to the first mixture and mixing evenly to obtain a second mixture; The second mixture is transferred to a treatment site, the water content is controlled to be 22% to 30%, and the mixture is treated at a temperature of 10 to 45° C. for 90 to 120 days to obtain greening planting soil; the greening planting soil leachate has a COD of less than 100 mg / L, a germination index of more than 80%, an organic matter content of more than 20 g / kg, available nitrogen of more than 40 mg / kg, available phosphorus of more than 30 mg / kg, available potassium of more than 1000 mg / kg, and loamy clay; Wherein, the biological agent is a mixture of bacterial agents and fungal solid agents of equal mass.

[0006] Preferably, the treatment site is a treatment pool, a treatment tank or a treatment box.

[0007] Preferably, the nutrient may include a mixture of at least one of organic fertilizer, straw, bran and rice bran and 0.1-0.3% nitrogen.

[0008] Preferably, the method for preparing the biological agent comprises the following steps: Collect water-based drilling solid waste samples from oil and gas drilling sites in desert areas; Adding various types of water-based drilling fluid additives and nitrogen sources to the sample to obtain a mixture with a carbon-nitrogen ratio of 15 to 20:1, adjusting the water content of the mixture to 28% to 30% and culturing the mixture at 10° C. to 20° C., eliminating strains without degradation ability and strains not suitable for low-temperature growth, gradually increasing the concentration of each water-based drilling fluid additive, and obtaining a mixture containing efficient degradation bacteria after acclimation; Separating and purifying the mixture containing the efficient degradation bacteria, and storing the separated and purified strains for later use; Prepare culture solutions containing a single water-based drilling fluid additive from the plurality of types of water-based drilling fluid additives respectively, inoculate the strain stored for standby into each culture solution, measure the degradation amount of the water-based drilling fluid additive contained in each culture solution, select strains with a degradation rate of the water-based drilling fluid additive in each culture solution greater than 7%, and obtain Zanthoxylum bungeanum; The bacterial preparation obtained by expanding the culture of Bacillus zanthoxylum is mixed with the fungal preparations prepared from Pseudomonas ovata WNF15 and Scopulariopsis brevicaulis WNF22 in equal amounts to obtain a biological preparation.

[0009] Preferably, the various types of water-based drilling fluid additives include carboxymethyl cellulose, polyacrylamide, sulfonated asphalt powder and sulfonated phenolic resin.

[0010] Preferably, the separation and purification of the mixture containing the high-efficiency degradation bacteria is carried out by using a dilution plate coating method and a plate streaking method.

[0011] Preferably, the separation and purification of the mixture containing the high-efficiency degrading bacteria includes: oscillating and mixing the mixture containing the high-efficiency degrading bacteria, and diluting it in different proportions to obtain diluted solutions of different concentrations; respectively spreading the diluted solutions of different concentrations on different beef extract peptone plates, and culturing them at a constant temperature of 10°C to 20°C for 24 to 36 hours; inoculating the colonies formed on different beef extract peptone plates onto new beef extract peptone plates and continuously streaking and separating them until colonies with consistent bacterial characteristics are obtained, thereby completing the separation and purification.

[0012] Preferably, the components of the culture fluid containing a single water-based drilling fluid additive include: a single water-based drilling fluid additive 0.4g / L~0.6g / L, NaHPO4 0.1g / L~0.3g / L, KH2PO4 0.9g / L~1.1g / L, ammonium acetate 0.2g / L~0.4g / L, MgSO4•7H2O0.4g / L~0.6g / L, MnSO4•H2O0.03g / L~0.05g / L, CaCl2 0.003g / L~0.005g / L, and the pH value of the culture fluid containing a single water-based drilling fluid additive is 7.0~7.2.

[0013] Preferably, the greening planting soil includes 20-50 g / kg organic matter, 30-60 mg / kg available phosphorus, 1000-1500 mg / kg available potassium, 40-80 mg / kg available nitrogen and loamy clay.

[0014] A method for biological treatment and resource utilization of water-based drilling solid waste in desert areas is suitable for water-based drilling solid waste with a pollutant content of 1.0-3.0%wt, an organic polymer content of 50%-60%wt in the pollutants, COD≤500mg / L, and sandy quality.

[0015] The beneficial effects of this technical solution are as follows: 1. The present invention provides a method for biological treatment and resource utilization of water-based drilling solid waste in desert areas. The process is simple and does not require pretreatment of the drilling waste mud such as coagulation, aeration and oxygenation, pH adjustment and temperature control, which is convenient for on-site operation and management.

[0016] 2. The method for biological treatment and resource utilization of water-based drilling solid waste in desert areas provided by the present invention can adapt to the treatment of sandy water-based drilling solid waste compared with the existing polymer and sulfonation solid waste biological enhancement treatment processes, and has a wide treatment temperature range, which is suitable for the medium and low temperature field environment in desert areas. DETAILED DESCRIPTION

[0017] The present invention is further described in detail below in conjunction with examples, but the embodiments of the present invention are not limited thereto.

[0018] Example 1 A method for biological treatment and resource utilization of water-based drilling solid waste in desert areas, which is suitable for water-based drilling solid waste with a pollutant content of 1.0-3.0%wt, an organic polymer content of 50%-60%wt in the pollutants, COD≤500mg / L, and sandy quality; specifically, water-based drilling solid waste in desert areas has a low pollutant content, and COD is generally less than 500mg / L, mainly organic polymers such as carboxymethyl cellulose, polyacrylamide, sulfonated asphalt powder and sulfonated phenolic resin. Here, the pollutant content is based on the mass of the water-based drilling solid waste, and the organic polymer content is based on the mass of the pollutants.

[0019] A method for biological treatment and resource utilization of water-based drilling solid waste in desert areas comprises the following steps: Preparation of biological agents for biological treatment and resource utilization of water-based drilling solid waste in desert areas; The biological agent and the water-based drilling solid waste in the desert area are mixed uniformly at a weight ratio of 0.1 to 1.0% wt to obtain a first mixture; Adding 1 to 5% wt of the nutrient agent to the first mixture and mixing evenly to obtain a second mixture; The second mixture is transferred to a treatment site, the water content is controlled to be 22% to 30%, and the mixture is treated at a temperature of 10 to 45° C. for 90 to 120 days to obtain greening planting soil; the greening planting soil leachate has a COD of less than 100 mg / L, a germination index of more than 80%, an organic matter content of more than 20 g / kg, available nitrogen of more than 40 mg / kg, available phosphorus of more than 30 mg / kg, available potassium of more than 1000 mg / kg, and loamy clay; The method for preparing the biological agent comprises the following steps: Collect water-based drilling solid waste samples from oil and gas drilling sites in desert areas; Adding various types of water-based drilling fluid additives and nitrogen sources to the sample to obtain a mixture with a carbon-nitrogen ratio of 15 to 20:1, adjusting the water content of the mixture to 28% to 30% and culturing the mixture at 10° C. to 20° C., eliminating strains without degradation ability and strains not suitable for low-temperature growth, gradually increasing the concentration of each water-based drilling fluid additive, and obtaining a mixture containing efficient degradation bacteria after acclimation; Separating and purifying the mixture containing the efficient degradation bacteria, and storing the separated and purified strains for later use; Prepare culture solutions containing a single water-based drilling fluid additive from the plurality of types of water-based drilling fluid additives respectively, inoculate the strain stored for standby into each culture solution, measure the degradation amount of the water-based drilling fluid additive contained in each culture solution, select strains with a degradation rate of the water-based drilling fluid additive in each culture solution greater than 7%, and obtain Zanthoxylum bungeanum; The bacterial preparation obtained by expanding the culture of Bacillus zanthoxylum is mixed with the fungal preparations prepared from Pseudomonas ovata WNF15 and Scopulariopsis brevicaulis WNF22 in equal amounts to obtain a biological preparation.

[0020] Example 2 A method for biological treatment and resource utilization of water-based drilling solid waste in desert areas comprises the following steps: The biological agent for treating water-based drilling solid waste in desert areas is prepared, wherein the biological agent is a mixture of Bacillus zanthoxylum YDB-10 or its bacterial suspension or culture fluid or fermentation product, and a fungal solid agent (Escherichia coli WNF15 and Scopulariopsis brevicaulis WNF22).

[0021] In this exemplary embodiment, the preparation of the biological agent may include the steps of: S01, collect samples. Collect water-based drilling solid waste samples from oil and gas drilling sites in desert areas. For example, sterile sampling bags can be used to collect water-based drilling solid waste samples from oil and gas drilling sites in desert areas. The water-based drilling solid waste samples can be old or fresh water-based drilling solid waste, water-based drilling solid waste-contaminated soil or water, and water-based drilling fluid-contaminated soil or water samples. The sample is the source of Bacillus zanthoxyli YDB-10.

[0022] S02, domestication of Bacillus zanthoxyli YDB-10.

[0023] A water-based drilling fluid additive and a nitrogen source are added to the collected water-based drilling solid waste sample to obtain a mixture. The water content of the mixture is adjusted to 28% to 30% with distilled water and cultured at 10°C to 20°C. Under the above conditions, bacteria that can degrade water-based drilling solid waste additives can be multiplied in large quantities. Then, microorganisms (strains) with no degradation ability and unsuitable for medium and low temperatures are eliminated, and the concentration of water-based drilling fluid additives is gradually increased to domesticate efficient degradation bacteria with strong degradation ability and tolerance to additives. The nitrogen source can be a nitrogen fertilizer, and the nitrogen fertilizer can be ammonium bicarbonate, ammonium sulfate, ammonium chloride, etc. The above nitrogen fertilizer is added to keep the carbon-nitrogen ratio in the mixture between 15 and 20:1 to facilitate the growth of the strain, for example, the carbon-nitrogen ratio can be 18:1. The above water content is controlled at 28% to 30%, and the temperature is controlled at 10°C to 20°C to provide a good strain screening environment. Too low a water content will cause the growth environment to be too dry, resulting in anoxia of the strain. The culture temperature is to screen strains suitable for medium and low temperature growth. Furthermore, the water content can be controlled at 29% and the culture temperature can be 15°C.

[0024] Furthermore, the water-based drilling fluid additive includes carboxymethyl cellulose, polyacrylamide, sulfonated asphalt powder and sulfonated phenolic resin.

[0025] S03, isolation and purification of Bacillus zanthoxyli YDB-10.

[0026] The mixture containing the high-efficiency degradation bacteria is separated and purified, and the separated and purified strains are stored for later use. Further, the separated and purified strains are inoculated on a slant culture medium and stored for later use. The vessels and water used in the separation and purification process are 100% sterile.

[0027] S04, rescreening Bacillus zanthoxyli YDB-10.

[0028] Prepare culture solutions containing a single water-based drilling fluid additive among multiple types of water-based drilling fluid additives (i.e., prepare a culture solution corresponding to each water-based drilling fluid additive, and each culture solution contains a single type of water-based drilling fluid additive), inoculate the isolated and purified strain in each culture solution, measure the reduction amount of the target substrate of the culture solution (the degradation amount of the water-based drilling fluid additive), and select the strain with a high degradation rate for various water-based drilling fluid additives, which is the Bacillus zanthoxyli YDB-10 of the present invention. The obtained Bacillus zanthoxyli YDB-10 is inoculated into a slant culture medium and stored at 4°C. In the process of selecting each water-based drilling fluid additive, a strain with a degradation rate greater than 7% can be selected, for example, a strain with a degradation rate greater than 10% can be selected.

[0029] In the above, when the water-based drilling fluid additive is carboxymethyl cellulose, polyacrylamide, sulfonated asphalt powder and sulfonated phenolic resin, the water-based drilling fluid additive culture solution can be a culture solution containing carboxymethyl cellulose, a culture solution containing polyacrylamide, a culture solution containing sulfonated asphalt powder and a culture solution containing sulfonated phenolic resin, respectively. The Bacillus zanthoxyli YDB-10 of the present invention can be obtained by selecting the strains with high degradation rates for the four additives.

[0030] In this embodiment, the separation and purification method can be separated and purified by dilution plate coating method and plate streaking method. Further, the separation and purification method can include: S100, the mixture containing the high-efficiency degradation bacteria is shaken and mixed, and diluted in different proportions to obtain dilution solutions of different concentrations. For example, the mixture containing the high-efficiency degradation bacteria is placed in a triangular flask with sterile water and shaken and mixed. The concentration range of the dilution solutions of different concentrations can be selected between a mass concentration range of 0.1% and 4%.

[0031] S200, coating the diluted solutions of different concentrations on different beef extract peptone plates respectively, and culturing at a constant temperature of 10°C to 20°C, for example, at 15°C for 24 to 36 hours.

[0032] S300, the colonies formed on different beef extract peptone plates are inoculated onto new beef extract peptone plates and continuously streaked and separated until colonies with consistent bacterial characteristics are obtained, thus completing separation and purification.

[0033] In an exemplary embodiment of the present invention, the culture fluid containing a single water-based drilling fluid additive may include: The culture medium containing carboxymethyl cellulose includes: carboxymethyl cellulose 0.4g / L~0.6g / L, NaHPO4 0.1g / L~0.3g / L, KH2PO4 0.9g / L~1.1g / L, ammonium acetate 0.2g / L~0.4g / L, MgSO4•7H2O 0.4g / L~0.6g / L, MnSO4•H2O 0.03g / L~0.05g / L, CaCl2 0.003g / L~0.005g / L. Further, carboxymethyl cellulose 0.5g / L, NaHPO4 0.2g / L, KH2PO4 1.0g / L, ammonium acetate 0.3g / L, MgSO4•7H2O 0.5g / L, MnSO4•H2O 0.04g / L, CaCL2 0.004g / L.

[0034] For the culture medium containing polyacrylamide: polyacrylamide 0.4g / L~0.6g / L, NaHPO4 0.1g / L~0.3g / L, KH2PO4 0.9g / L~1.1g / L, ammonium acetate 0.2g / L~0.4g / L, MgSO4•7H2O 0.4g / L~0.6g / L, MnSO4•H2O 0.03g / L~0.05g / L, CaCl2 0.003g / L~0.005g / L. Further, polyacrylamide 0.5g / L, NaHPO4 0.2g / L, KH2PO4 1.0g / L, ammonium acetate 0.3g / L, MgSO4•7H2O 0.5g / L, MnSO4•H2O 0.04g / L, CaCl2 0.004g / L.

[0035] The culture medium containing sulfonated asphalt powder includes: sulfonated asphalt powder 0.4g / L~0.6g / L, NaHPO4 0.1g / L~0.3g / L, KH2PO4 0.9g / L~1.1g / L, ammonium acetate 0.2g / L~0.4g / L, MgSO4•7H2O 0.4g / L~0.6g / L, MnSO4•H2O 0.03g / L~0.05g / L, and CaCl2 0.003g / L~0.005g / L. Further, sulfonated asphalt powder 0.5 g / L, NaHPO4 0.2 g / L, KH2PO4 1.0 g / L, ammonium acetate 0.3 g / L, MgSO4•7H2O 0.5 g / L, MnSO4•H2O 0.04 g / L, CaCL2 0.004 g / L.

[0036] The culture medium containing sulfonated phenolic resin includes: sulfonated phenolic resin 0.4g / L~0.6g / L, NaHPO4 0.1g / L~0.3g / L, KH2PO4 0.9g / L~1.1g / L, ammonium acetate 0.2g / L~0.4g / L, MgSO4•7H2O 0.4g / L~0.6g / L, MnSO4•H2O 0.03g / L~0.05g / L, CaCl2 0.003g / L~0.005g / L. Further, sulfonated phenolic resin 0.5 g / L, NaHPO4 0.2 g / L, KH2PO4 1.0 g / L, ammonium acetate 0.3 g / L, MgSO4•7H2O 0.5 g / L, MnSO4•H2O 0.04 g / L, and CaCL2 0.004 g / L.

[0037] As mentioned above, for the culture solution containing each additive, the pH value may be 7.0 to 7.2. Each culture medium is prepared by sterilizing at 121° C. for 20 to 30 minutes.

[0038] In this embodiment, the beef extract peptone medium and the slant medium may include: peptone 10g / L, sodium chloride 5g / L, beef extract 5g / L. The pH value of the slant medium may be 7.0-7.2, and the medium may be prepared after sterilization at 121°C for 30min. Of course, other types of separation medium may also be selected to replace the beef extract peptone medium during the separation and purification process of the strain of the invention.

[0039] In this embodiment, the Bacillus zanthoxyli YDB-10 is an aerobic type, and further a strictly aerobic type, and can be used only under aerobic conditions.

[0040] In this embodiment, the Bacillus zanthoxyli YDB-10 can grow at a pH of 4.0 to 10.0, a salt concentration of 0% to 4%, and a temperature of 10 to 45° C. Further, it can grow at a pH of 6.5 to 7.5, a temperature of 15 to 20° C., and a salt concentration of 0.5% to 1%.

[0041] In this embodiment, the colonies formed by culturing the Bacillus zanthoxyli YDB-10 on the beef extract peptone medium for 24 hours are round or irregular, and the colonies formed after 48 hours are round, white, with a diameter of 1.5 mm to 2.0 mm, neat edges, and flat and moist.

[0042] As described above, the Bacillus zanthoxyli YDB-10 of the present invention can simultaneously degrade a variety of drilling fluid additives such as carboxymethyl cellulose, polyacrylamide, sulfonated asphalt powder and sulfonated phenolic resin, and the isolated strain has strong alkali and salt resistance, can play a role in degrading drilling solid waste additives under pH 5.0-10.0 and salt concentration 0%-4%, and has a wide growth temperature range (10°C-45°C). In addition, the Bacillus zanthoxyli can repair the medium contaminated by water-based drilling fluid solid waste, and the medium is soil, water and air. The Bacillus zanthoxyli YDB-10 can degrade the total organic carbon (TOC) of water-based drilling solid waste. The strain obtained by taming and separation is cultured and expanded as a biological agent for the biological treatment and resource utilization of water-based drilling solid waste in desert areas. Here, the culture expansion includes the steps of: inoculating the obtained strain into beef extract peptone liquid culture medium for activation for 16 hours, inoculating into expansion culture medium (5g peptone, 10g sucrose, 1g beef extract, 10g sodium chloride, 1L tap water, pH 7.0, sterilized at 121°C for 30min) at a 5% inoculation amount, 10°C to 20°C, ventilation volume 1.2L•min-1, when the bacterial liquid OD600 reaches 1.0, it is liquid biological preparation 1; mixing the liquid biological preparation 1 into organic fertilizer at 25% can prepare solid biological preparation 1. Mixing biological preparation 1 with fungal solid bacterial agents (Escherichia coli WNF15, Scopulariopsis brevicaulis WNF22) of equal mass, a water-based drilling solid waste treatment biological preparation for desert areas is obtained.

[0043] Add 1 to 5% wt of the nutrients in the first mixture and mix evenly to obtain a second mixture.

[0044] The second mixture is transferred to a treatment site, the water content is controlled to be 22% to 30%, and the mixture is treated at a temperature of 10 to 45°C for 90 to 120 days to obtain greening soil. Here, compared with the existing polymer and sulfonated solid waste bio-enhanced treatment processes, it can adapt to the treatment of sandy water-based drilling solid waste, and the treatment temperature range is wide, which is suitable for the low-temperature field environment in desert areas. The treatment site can be a treatment pool, a treatment tank or a treatment box. The nutrient agent may include at least one of organic fertilizer, straw, bran and rice bran and a mixture of 0.1 to 0.3% nitrogen. The greening soil leachate has a COD of less than 100 mg / L, a germination index of more than 80%, an organic matter content of more than 20g / kg, available nitrogen of more than 40mg / kg, available phosphorus of more than 30mg / kg, available potassium of more than 1000mg / kg, and a loamy clay texture. The above ranges all meet the requirements of the CJ / T340-2016 Class III standard for greening soil.

[0045] In summary, the beneficial effects of the present invention may include at least one of the following: (1) The method for biological treatment of water-based drilling solid waste in desert areas of the present invention is simple in process and does not require pretreatment of the drilling waste mud such as coagulation, aeration and oxygenation, pH adjustment and temperature adjustment, which is convenient for on-site operation and management; (2) Compared with the existing polymer and sulfonation solid waste bio-enhanced treatment processes, the method for biological treatment and resource utilization of water-based drilling solid waste in desert areas of the present invention can adapt to the treatment of sandy water-based drilling solid waste and has a wide treatment temperature range, which is suitable for the low and medium temperature field environment in desert areas.

[0046] Finally, it should be noted that the above embodiments are only used to illustrate rather than limit the technical solutions of the present invention. Although the present invention has been described in detail with reference to the above embodiments, those skilled in the art should understand that the present invention can still be modified or replaced by equivalents. Any modification or partial replacement that does not depart from the spirit and scope of the present invention should be included in the scope of the claims of the present invention.

Claims

1. A method for biological treatment and resource utilization of water-based drilling solid waste in desert areas, characterized in that: The following steps are involved: Preparation of biological agents for biological treatment and resource utilization of water-based drilling solid waste in desert areas; The biological agent and the water-based drilling solid waste in the desert area are mixed uniformly at a weight ratio of 0.1 to 1.0% wt to obtain a first mixture; Adding 1 to 5% wt of the nutrient agent to the first mixture and mixing evenly to obtain a second mixture; The second mixture is transferred to a treatment site, the water content is controlled to be 22% to 30%, and the mixture is treated at a temperature of 10 to 45° C. for 90 to 120 days to obtain greening planting soil; the greening planting soil leachate has a COD of less than 100 mg / L, a germination index of more than 80%, an organic matter content of more than 20 g / kg, available nitrogen of more than 40 mg / kg, available phosphorus of more than 30 mg / kg, available potassium of more than 1000 mg / kg, and loamy clay; Wherein, the biological agent is a mixture of bacterial agents and fungal solid agents of equal mass.

2. The method for biological treatment and resource utilization of water-based drilling solid waste in desert areas according to claim 1 is characterized by: The treatment place is a treatment pool, a treatment tank or a treatment box.

3. The method for biological treatment and resource utilization of water-based drilling solid waste in desert areas according to claim 1 is characterized by: The nutrient may include a mixture of at least one of organic fertilizer, straw, bran and rice bran and 0.1-0.3% nitrogen.

4. The method for biological treatment and resource utilization of water-based drilling solid waste in desert areas according to claim 1 is characterized by: The preparation method of the biological agent comprises the following steps: Collect water-based drilling solid waste samples from oil and gas drilling sites in desert areas; Adding various types of water-based drilling fluid additives and nitrogen sources to the sample to obtain a mixture with a carbon-nitrogen ratio of 15 to 20:1, adjusting the water content of the mixture to 28% to 30% and culturing the mixture at 10° C. to 20° C., eliminating strains without degradation ability and strains not suitable for low-temperature growth, gradually increasing the concentration of each water-based drilling fluid additive, and obtaining a mixture containing efficient degradation bacteria after acclimation; Separating and purifying the mixture containing the efficient degradation bacteria, and storing the separated and purified strains for later use; Prepare culture solutions containing a single water-based drilling fluid additive from the plurality of types of water-based drilling fluid additives respectively, inoculate the strain stored for standby into each culture solution, measure the degradation amount of the water-based drilling fluid additive contained in each culture solution, select strains with a degradation rate of the water-based drilling fluid additive in each culture solution greater than 7%, and obtain Zanthoxylum bungeanum; The bacterial preparation obtained by expanding the culture of Bacillus zanthoxylum is mixed with the fungal preparations prepared from Pseudomonas ovata WNF15 and Scopulariopsis brevicaulis WNF22 in equal amounts to obtain a biological preparation.

5. The method for biological treatment and resource utilization of water-based drilling solid waste in desert areas according to claim 1 is characterized by: The various types of water-based drilling fluid additives include carboxymethyl cellulose, polyacrylamide, sulfonated asphalt powder and sulfonated phenolic resin.

6. The method for biological treatment and resource utilization of water-based drilling solid waste in desert areas according to claim 1 is characterized by: The separation and purification of the mixture containing the high-efficiency degradation bacteria adopts the dilution plate coating method and the plate streaking method.

7. The method for biological treatment and resource utilization of water-based drilling solid waste in desert areas according to claim 1 is characterized by: The separation and purification of the mixture containing the high-efficiency degradation bacteria comprises: oscillating and mixing the mixture containing the high-efficiency degradation bacteria, and obtaining dilution solutions of different concentrations after diluting them in different proportions; respectively applying the dilution solutions of different concentrations on different beef extract peptone plates, and culturing them at a constant temperature of 10°C to 20°C for 24 to 36 hours; inoculating the colonies formed on the different beef extract peptone plates onto new beef extract peptone plates and continuously streaking and separating them until colonies with consistent bacterial characteristics are obtained, thereby completing the separation and purification.

8. The method for biological treatment and resource utilization of water-based drilling solid waste in desert areas according to claim 1 is characterized by: The components of the culture fluid containing a single water-based drilling fluid additive include: a single water-based drilling fluid additive 0.4g / L~0.6g / L, NaHPO4 0.1g / L~0.3g / L, KH2PO4 0.9g / L~1.1g / L, ammonium acetate 0.2g / L~0.4g / L, MgSO4•7H2O0.4g / L~0.6g / L, MnSO4•H2O0.03g / L~0.05g / L, CaCl2 0.003g / L~0.005g / L, and the pH value of the culture fluid containing a single water-based drilling fluid additive is 7.0~7.

2.

9. The method for biological treatment and resource utilization of water-based drilling solid waste in desert areas according to claim 1 is characterized by: The greening planting soil comprises 20-50 g / kg organic matter, 30-60 mg / kg effective phosphorus, 1000-1500 mg / kg quick-acting potassium, 40-80 mg / kg quick-acting nitrogen and loamy clay.

10. A method for biological treatment and resource utilization of water-based drilling solid waste in desert areas is suitable for water-based drilling solid waste with a pollutant content of 1.0-3.0%wt, an organic polymer content of 50%-60%wt in the pollutants, COD≤500mg / L, and sandy quality.