Compound missible oil insecticide for pest control and preparation method thereof

Abstract

The invention provides a compound missible oil insecticide for pest control and a preparation method thereof, and relates to the field of agricultural pest control, and the compound missible oil insecticide comprises at least two insecticide active compounds with different action mechanisms, pea albumin, a pichia pastoris fermentation product and a missible oil matrix. The preparation method of the compound missible oil insecticide comprises the following steps: culturing pichia pastoris, preparing a pea albumin solution, and mixing the insecticide active compound, the pea albumin solution, the pichia pastoris fermentation product and the solvent and the emulsifier in the missible oil matrix to prepare the insecticide. According to the invention, the pest control effect can be improved, the drug resistance development of a single insecticide is reduced, and the efficient dispersion and stability of the insecticide are ensured.

Description

Technical Field

[0001] The present invention relates to the field of agricultural pest control, and more specifically, to a compound emulsifiable concentrate insecticide for pest control and a preparation method thereof. Background Art

[0002] In agricultural planting, pest control is an important link. Traditional pest control methods often rely on chemical pesticides with a single mechanism of action. Although these pesticides can effectively control pests in the short term, long-term use will cause pests to develop resistance, reduce the control effect, and have a negative impact on the environment and non-target organisms. In addition, a single pesticide can often only target specific types of pests, and has limited control effects on multiple types of pests. In order to improve the efficiency and effectiveness of pest control and reduce the use of chemical pesticides, the development of new compound pesticides has become a research focus. This compound pesticide can combine pesticide original drugs with different mechanisms of action and biologically active ingredients in order to achieve the purpose of increasing efficiency, reducing dosage, and reducing the development of resistance.

[0003] In the process of implementing the embodiments of the present invention, the inventors found that there are at least the following problems or defects in the prior art: the existing compound pesticides often lack the scientific ratio of pesticides with different action mechanisms and the effective use of biologically active ingredients, resulting in poor control effects or excessive costs. In addition, the imperfection of the preparation method also affects the stability and dispersibility of the pesticide, limiting its effectiveness in practical applications. Summary of the invention

[0004] The invention provides a compound emulsifiable concentrate insecticide for pest control and a preparation method thereof.

[0005] In a first aspect of the present invention, a compounded emulsifiable concentrate insecticide for pest control is provided, the compounded emulsifiable concentrate insecticide comprising an active ingredient, pea albumin, a Pichia yeast fermentation product and an emulsifiable concentrate base, wherein the active ingredient comprises at least two insecticide original drugs with different action mechanisms; Based on the total mass of the compound emulsifiable concentrate insecticide, the mass fraction of pea albumin is 1%-5%, and the mass fraction of Pichia yeast fermentation product is 3%-8%; The two pesticide technicals with different action mechanisms are pyrethroid technical and organophosphorus technical, and the mass ratio of pyrethroid technical to organophosphorus technical is 1:2; The emulsifiable concentrate base comprises a solvent and an emulsifier, wherein the solvent is selected from at least one of xylene, toluene and cyclohexanone, and the emulsifier is a mixture of a nonionic emulsifier and an anionic emulsifier in a mass ratio of 3:1.

[0006] Furthermore, based on the total mass of the compound emulsifiable concentrate insecticide, the mass fraction of the solvent is 50%-70%, and the mass fraction of the emulsifier is 8%-15%.

[0007] In a second aspect of the present invention, a method for preparing a compound emulsifiable concentrate insecticide for pest control is provided, which specifically comprises the following steps: Cultivating Pichia pastoris: inoculating Pichia pastoris into a culture medium for fermentation to obtain a Pichia pastoris fermentation broth, performing solid-liquid separation on the fermentation broth, and collecting a fermentation product; preparing a pea albumin solution: dissolving pea albumin in a buffer solution to prepare a pea albumin solution; Mixing: adding the original insecticide, pea albumin solution, Pichia yeast fermentation product, and the solvent and emulsifier in the emulsifiable concentrate base into the reaction kettle in proportion, stirring and mixing evenly to obtain the composite emulsifiable concentrate insecticide.

[0008] Furthermore, in the step of culturing Pichia pastoris, the nutrients of the culture medium include a carbon source, a nitrogen source, inorganic salts and trace elements, the fermentation culture temperature is 28° C.-32° C., the pH is 5.0-6.0, and the fermentation time is 36-48 hours.

[0009] Furthermore, in the step of preparing the pea albumin solution, the buffer solution is a phosphate buffer solution with a pH of 6.5-7.5, and the concentration of the pea albumin solution is 5%-10% w / v.

[0010] Furthermore, in the mixing step, the stirring speed is 300-500 rpm, and the stirring time is 30-60 minutes.

[0011] Furthermore, before the mixing step, the original pesticide is pretreated and ground to a particle size of less than 10 microns to improve its dispersibility in the compound emulsifiable concentrate pesticide.

[0012] The present invention has at least the following beneficial effects: the compounded emulsifiable concentrate insecticide provided by the present invention can effectively target a variety of pests by combining at least two insecticide original drugs with different mechanisms of action, thereby reducing the development of single insecticide resistance. At the same time, the addition of pea albumin and Pichia yeast fermentation products, these two biologically active ingredients can enhance the biocompatibility and environmental friendliness of the insecticide, and reduce the impact on non-target organisms and the environment. The optimized formula of the emulsifiable concentrate matrix and the improvement of the preparation method can improve the dispersibility and stability of the insecticide, ensuring that the insecticide can exert the best effect under different environmental conditions.

[0013] In addition, the preparation method of the present invention can ensure that the particle size of the pesticide original drug is less than 10 microns by accurately controlling the fermentation conditions of Pichia yeast and the preparation of the pea albumin solution, as well as the stirring speed and time during the mixing process, thereby improving its dispersibility in the emulsifiable concentrate matrix and enhancing the penetration and adhesion of the pesticide. This optimized preparation process can not only improve the efficacy of the pesticide, but also reduce the use of chemical pesticides and reduce agricultural production costs, which is of great significance to environmental protection and sustainable development. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] The above and other objects, features and advantages of the exemplary embodiments of the present invention will become readily understood by reading the detailed description below with reference to the accompanying drawings. In the accompanying drawings, several embodiments of the present invention are shown in an exemplary and non-limiting manner, in which: Figure 1 A schematic flow chart of a method for preparing a compound emulsifiable concentrate insecticide for pest control provided in one embodiment of the present invention. DETAILED DESCRIPTION

[0015] The principles and spirit of the present invention will be described below with reference to several exemplary embodiments. It should be understood that these embodiments are provided only to enable those skilled in the art to better understand and implement the present invention, and are not intended to limit the scope of the present invention in any way. On the contrary, these embodiments are provided to make the present invention more thorough and complete, and to fully convey the scope of the present invention to those skilled in the art.

[0016] In order to make the purpose, technical scheme and advantages of the present invention clearer, the present invention is further described in detail below in conjunction with the embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention. All features disclosed in this specification, or steps in all methods or processes disclosed, except for mutually exclusive characteristics and / or steps, can be combined in any way, unless otherwise described, and can be replaced by other equivalent or alternative features with similar purposes, that is, unless otherwise described, each feature is only one embodiment in a series of equivalent or similar features.

[0017] In some embodiments, a compounded emulsifiable concentrate insecticide for pest control comprises an active ingredient, pea albumin, a Pichia yeast fermentation product, and an emulsifiable concentrate base, wherein the active ingredient comprises at least two insecticide technicals with different mechanisms of action.

[0018] It should be noted that the present invention relates to a compound emulsifiable concentrate insecticide, which comprises an active ingredient, pea albumin, a Pichia fermentation product and an emulsifiable concentrate base. The active ingredient refers to at least two insecticide original drugs with different mechanisms of action, and this design aims to improve the pest control effect through the synergistic effect of different mechanisms. Pea albumin is a plant-derived protein, and the Pichia fermentation product is a biological fermentation product, both of which can enhance the biocompatibility and environmental friendliness of the insecticide. The emulsifiable concentrate base is a carrier of the insecticide, including a solvent and an emulsifier, which is used to dissolve and disperse the original insecticide.

[0019] Specifically, the active ingredients contain at least two pesticide technicals with different mechanisms of action, such as pyrethroid technicals and organophosphorus technicals, which can be mixed in a mass ratio of 1:2. The mass fraction of pea albumin is controlled at 1%-5%, and the mass fraction of Pichia yeast fermentation products is controlled at 3%-8%. The solvent in the emulsifiable concentrate matrix can be at least one of xylene, toluene or cyclohexanone, and the emulsifier is a mixture of non-ionic emulsifier and anionic emulsifier in a mass ratio of 3:1. Such a ratio and ingredient selection can ensure the effectiveness and environmental safety of the pesticide.

[0020] Preferably, we can further refine the preparation method. For example, the mass fraction of the solvent can be controlled at 50%-70%, and the mass fraction of the emulsifier can be controlled at 8%-15%. In the mixing step, the stirring speed can be set at 300-500 rpm, and the stirring time can be controlled at 30-60 minutes. Such operating conditions can ensure that the pesticide original drug is evenly dispersed in the emulsifiable concentrate matrix, thereby improving the stability and effect of the pesticide.

[0021] Furthermore, the pesticide technical can be pre-treated before mixing and ground to a particle size of less than 10 microns to further improve its dispersibility in the emulsifiable concentrate matrix.

[0022] In some embodiments, based on the total mass of the compound emulsifiable concentrate insecticide, the mass fraction of pea albumin is 1%-5%, and the mass fraction of Pichia pastoris fermentation product is 3%-8%.

[0023] It should be noted that in the compound emulsifiable concentrate insecticide for pest control of the present embodiment, the mass fractions of pea albumin and Pichia yeast fermentation product have a specific ratio. Pea albumin is a protein extracted from peas, which has biological activity and can enhance the adhesion and permeability of the insecticide. Pichia yeast fermentation product is a biologically active substance obtained through the Pichia yeast fermentation process, which can improve the biocompatibility and environmental safety of the insecticide.

[0024] Specifically, the mass fraction of pea albumin in the total mass of the pesticide is controlled between 1% and 5%. This ratio can ensure that the pesticide has good adhesion and permeability without causing too much burden on the environment. The mass fraction of Pichia yeast fermentation products is controlled between 3% and 8%. This ratio can ensure the biological activity and environmental friendliness of the pesticide. This ratio setting is based on experimental data and effect evaluation, and aims to balance the insecticide effect and environmental impact.

[0025] Preferably, we can further refine the specific settings of these ratios. For example, the mass fraction of pea albumin can be preferably set between 2% and 4%, and the mass fraction of Pichia yeast fermentation product can be preferably set between 4% and 7%. The adjustment of these ratios can be optimized according to the specific pest types and control requirements.

[0026] Furthermore, it is also possible to consider using proteins from other sources or biofermentation products as alternatives to adapt to different application scenarios and environmental conditions. Through these refined operating steps and parameter selections, it is possible to ensure that the pesticide can achieve the best effect under different conditions.

[0027] In some embodiments, the two insecticide technicals with different action mechanisms are pyrethroid technical and organophosphorus technical, and the mass ratio of pyrethroid technical to organophosphorus technical is 1:2.

[0028] It should be noted that in the compound emulsifiable concentrate insecticide involved in this embodiment, the two insecticides with different mechanisms of action are specifically designated as pyrethroids and organophosphorus. Pyrethroids are insecticides synthesized by simulating the chemical structure of pyrethrins in nature, while organophosphorus is a class of organic compounds containing phosphorus, which have toxic effects on pests through different mechanisms. This combination is intended to improve the insecticidal effect and reduce the development of drug resistance.

[0029] Specifically, the mass ratio of pyrethroid technical to organophosphorus technical is set at 1:2, that is, in the insecticide formula, the amount of organophosphorus technical is twice that of pyrethroid technical. This ratio is based on experimental research and field tests, and is intended to ensure the synergistic effect of the two insecticides to achieve the best pest control effect. Through this specific ratio, the efficiency of the insecticide can be improved, while reducing the use of each individual insecticide and reducing the potential impact on the environment.

[0030] Preferably, we can further refine the specific selection and use of these two pesticide technicals. For example, cypermethrin or flucythrin can be selected as the pyrethroid technical, and malathion can be selected as the organophosphorus technical. In practical applications, the specific types and proportions of these two pesticides can be adjusted according to the types of pests and their resistance to pesticides to achieve the best control effect.

[0031] Furthermore, other types of pesticides or synergists can be added to further improve the effect and adaptability of the pesticide. Through these detailed operation steps and parameter selection, it can be ensured that the pesticide can play the best effect under different conditions.

[0032] In some embodiments, the emulsifiable concentrate base includes a solvent and an emulsifier, the solvent is selected from at least one of xylene, toluene, and cyclohexanone, and the emulsifier is a mixture of a nonionic emulsifier and an anionic emulsifier in a mass ratio of 3:1.

[0033] It should be noted that this embodiment describes the composition of the emulsifiable concentrate matrix in the compound emulsifiable concentrate insecticide, and the matrix includes a solvent and an emulsifier. The solvent is a liquid used to dissolve the original pesticide, while the emulsifier is used to help the pesticide disperse in water to form a uniform emulsion. Nonionic emulsifiers and anionic emulsifiers are two common types of emulsifiers, which stabilize the emulsion based on different chemical structures and surface active properties.

[0034] Specifically, the solvent in the emulsifiable concentrate base can be selected from at least one of xylene, toluene or cyclohexanone. These solvents have different boiling points and solubility characteristics and can be selected according to the properties of the pesticide original and environmental requirements. The emulsifier is a mixture of a nonionic emulsifier and an anionic emulsifier in a mass ratio of 3:1, which can provide good emulsion stability and dispersibility. Nonionic emulsifiers generally have good compatibility and low foaming tendency, while anionic emulsifiers provide stronger emulsification ability.

[0035] Preferably, we can further refine the composition and preparation process of the emulsifiable concentrate matrix. For example, the mass fraction of the solvent can be controlled at 50%-70%, which can ensure the effective dissolution of the pesticide original drug while maintaining the stability of the emulsion. The mass fraction of the emulsifier can be controlled at 8%-15% to ensure uniform dispersion of the emulsion. In the actual preparation process, the types and proportions of the solvent and emulsifier can be adjusted according to the specific application requirements and environmental conditions.

[0036] Furthermore, other types of emulsifiers, such as cationic emulsifiers or zwitterionic emulsifiers, can also be considered to meet different formulation requirements. Through these detailed operating steps and parameter selection, it can be ensured that the emulsifiable concentrate base can provide the best dispersion and stability performance under different conditions.

[0037] In some embodiments, based on the total mass of the compound emulsifiable concentrate insecticide, the mass fraction of the solvent is 50%-70%, and the mass fraction of the emulsifier is 8%-15%.

[0038] It should be noted that this embodiment describes in detail the mass fraction range of the solvent and emulsifier in the compound emulsifiable concentrate insecticide. The solvent is the liquid used to dissolve the original pesticide in the emulsifiable concentrate matrix, while the emulsifier is a substance that helps the pesticide disperse in water to form an emulsion. The mass fraction refers to the ratio of the mass of a certain component to the total mass of the mixture, and is an important parameter for measuring the proportion of the components.

[0039] Specifically, the mass fraction of the solvent in the total mass of the compound emulsifiable concentrate insecticide is controlled between 50% and 70%, and the mass fraction of the emulsifier is controlled between 8% and 15%. This ratio is set to ensure the effective dissolution of the original insecticide and the stability and dispersibility of the emulsion. A higher proportion of the solvent helps to improve the penetration of the insecticide, while an appropriate proportion of the emulsifier ensures the uniformity and durability of the emulsion.

[0040] Preferably, we can further refine the specific settings of these ratios. For example, the mass fraction of the solvent can be preferably set between 55% and 65%, which can provide good solubility and emulsion stability. The mass fraction of the emulsifier can be preferably set between 10% and 14% to ensure uniform dispersion and long-term stability of the emulsion. In practical applications, the specific ratio of the solvent and the emulsifier can be adjusted according to the specific use environment of the pesticide and the type of target pest to achieve the best control effect.

[0041] Furthermore, it is also possible to consider using different combinations of solvents and emulsifiers, or adjusting their mass ratios, to adapt to different formulation requirements and environmental conditions. Through these detailed operating steps and parameter selections, it is possible to ensure that the compound emulsifiable concentrate insecticide can achieve the best effect under different conditions.

[0042] In some embodiments, the preparation method of the compound emulsifiable concentrate insecticide for pest control is as follows: Figure 1 As shown, the following steps are included: Step 101, culturing Pichia pastoris: inoculating Pichia pastoris into a culture medium containing specific nutrients, fermenting and culturing under suitable temperature and pH conditions to obtain Pichia pastoris fermentation broth, performing solid-liquid separation on the fermentation broth, and collecting fermentation products; Step 102, preparing a pea albumin solution: dissolving pea albumin in an appropriate amount of buffer solution to prepare a pea albumin solution of a certain concentration; Step 103, mixing: adding the original pesticide, pea albumin solution, Pichia yeast fermentation product, and the solvent and emulsifier in the emulsifiable concentrate base into the reactor in proportion, stirring and mixing at a certain speed to obtain the composite emulsifiable concentrate pesticide.

[0043] It should be noted that this embodiment describes in detail the preparation method of the compound emulsifiable concentrate insecticide, including the steps of culturing Pichia pastoris, preparing a pea albumin solution, and mixing the components. Pichia pastoris is a commonly used fermentation microorganism that can produce a variety of biologically active substances. The pea albumin solution is a liquid obtained by dissolving pea albumin in a buffer solution.

[0044] Specifically, the preparation method first includes culturing Pichia pastoris, that is, inoculating Pichia pastoris into a culture medium containing specific nutrients, and fermenting and culturing under suitable temperature and pH conditions to obtain Pichia pastoris fermentation broth, and then performing solid-liquid separation on the fermentation broth to collect the fermentation product. Next, a pea albumin solution is prepared, and pea albumin is dissolved in an appropriate amount of buffer solution to prepare a pea albumin solution of a certain concentration. Finally, the insecticide original drug, pea albumin solution, Pichia pastoris fermentation product, and the solvent and emulsifier in the emulsifiable concentrate matrix are sequentially added to a reactor in proportion, and stirred and mixed evenly at a certain speed to obtain a composite emulsifiable concentrate insecticide.

[0045] Preferably, we can further refine the specific operations of these steps. For example, in the step of culturing Pichia pastoris, the specific nutrients may include a carbon source, a nitrogen source, an inorganic salt and trace elements, the suitable temperature may be controlled at 28°C-32°C, the pH may be controlled at 5.0-6.0, and the fermentation time may be controlled at 36-48 hours. In the step of preparing the pea albumin solution, the buffer solution may be a phosphate buffer solution, the pH may be controlled at 6.5-7.5, and the concentration of the pea albumin solution may be controlled at 5%-10% w / v. In the mixing step, the stirring speed may be controlled at 300-500 rpm, and the stirring time may be controlled at 30-60 minutes. The setting of these parameters can ensure the effective fermentation of Pichia pastoris and the proper preparation of the pea albumin solution, as well as the uniform mixing of the final pesticide.

[0046] Furthermore, it is also possible to consider using other types of buffer solutions or adjusting the stirring speed and time to adapt to different production conditions and improve efficiency. Through these refined operating steps, the preparation process of the pesticide can be ensured to be both efficient and controllable.

[0047] In some embodiments, in the step of culturing Pichia pastoris, the specific nutrients include a carbon source, a nitrogen source, an inorganic salt and trace elements, the suitable temperature is 28° C.-32° C., the pH is 5.0-6.0, and the fermentation time is 36-48 hours.

[0048] It should be noted that the present embodiment describes in detail the specific conditions for culturing Pichia pastoris, including nutrients, temperature, pH value and fermentation time. Pichia pastoris is a yeast commonly used in industrial fermentation that can produce a variety of useful metabolites. Specific nutrients refer to those substances that support the growth and metabolism of microorganisms, including carbon sources, nitrogen sources, inorganic salts and trace elements.

[0049] Specifically, when culturing Pichia pastoris, the yeast needs to be inoculated into a culture medium containing a carbon source, a nitrogen source, inorganic salts and trace elements. These nutrients are necessary for the growth of microorganisms. The carbon source provides energy, the nitrogen source provides the nitrogen required for the synthesis of proteins and other biological molecules, and the inorganic salts and trace elements participate in the activity and metabolic processes of various enzymes. The suitable temperature range is 28°C-32°C, which is conducive to the growth and metabolic activities of Pichia pastoris. The pH value is controlled at 5.0-6.0, and this pH range can ensure the optimal activity of the enzyme system of Pichia pastoris. The fermentation time is controlled at 36-48 hours. This period of time can ensure that Pichia pastoris is fully fermented and produces sufficient metabolites.

[0050] Preferably, we can further refine the conditions for culturing Pichia pastoris. For example, the carbon source can be glucose or other fermentable sugars, and the nitrogen source can be yeast extract or peptone. Inorganic salts can include phosphates, magnesium sulfate, etc., and trace elements can include iron, zinc, copper, etc. The temperature can be preferably controlled at 30°C, which is the optimal temperature for the growth of Pichia pastoris. The pH value can be preferably controlled at 5.5, at which the metabolic activity of Pichia pastoris is most active. The fermentation time can be preferably controlled at 40 hours, which can ensure that Pichia pastoris is fully fermented while avoiding the decomposition of metabolites caused by excessive fermentation.

[0051] Furthermore, it is also possible to consider using other types of culture media or adjusting the culture conditions to adapt to different production requirements and increase the yield of metabolites. Through these refined operating steps, the culture process of Pichia pastoris can be ensured to be both efficient and controllable.

[0052] In some embodiments, in the step of preparing the pea albumin solution, the buffer solution is a phosphate buffer solution with a pH of 6.5-7.5, and the concentration of the pea albumin solution is 5%-10% w / v.

[0053] It should be noted that this embodiment describes in detail the specific steps of preparing the pea albumin solution, including the selection of the buffer solution and the concentration of the pea albumin solution. A buffer solution is a solution that can resist pH changes, usually composed of a weak acid and its salt or a weak base and its salt, and is used to maintain the pH stability of the solution. Phosphate buffer solution is a commonly used buffer solution, which is widely used in biochemical experiments and industrial production due to its stability and biocompatibility.

[0054] Specifically, in the process of preparing the pea albumin solution, the buffer solution used is a phosphate buffer solution, and its pH value is controlled between 6.5-7.5. This pH range is close to neutral, which is conducive to maintaining the biological activity and stability of pea albumin. The concentration of the pea albumin solution is controlled at 5%-10% w / v, that is, 5 to 10 grams of pea albumin are contained in every 100 ml of solution. This concentration range can ensure that pea albumin is fully dissolved in the solution while maintaining its biological activity.

[0055] Preferably, we can further refine the preparation steps of the pea albumin solution. For example, the pH value of the phosphate buffer solution can be preferably controlled at 7.0, which is the most stable pH value of pea albumin. The concentration of the pea albumin solution can be preferably controlled at 7.5% w / v, which can ensure the solubility of pea albumin and maintain its biological activity. In the actual preparation process, the concentration of the solution can be adjusted according to the source and quality of pea albumin and the desired application purpose.

[0056] Furthermore, other types of buffer solutions, such as Tris-HCl buffer solution or acetate buffer solution, can also be considered to adapt to different experimental conditions or production requirements. Through these refined operating steps, it can be ensured that the preparation of pea albumin solution is both efficient and meets specific application requirements.

[0057] In some embodiments, in the mixing step, the stirring speed is 300-500 rpm, and the stirring time is 30-60 minutes.

[0058] It should be noted that the present embodiment describes in detail the specific operating conditions in the mixing step, including the stirring speed and stirring time. The stirring speed refers to the number of rotations per minute of the stirrer, which is an important parameter in the mixing process and affects the uniformity and efficiency of the mixing. The stirring time refers to the duration of the mixing operation, which also affects the uniformity of the mixing and the quality of the final product.

[0059] Specifically, in the mixing step, the stirring speed is controlled at 300-500 rpm. This speed range can ensure that the various components are fully mixed in the emulsifiable concentrate base to form a uniform emulsion. The stirring time is controlled at 30-60 minutes, which can ensure that all components are fully dispersed and achieve the desired mixing effect. The setting of these parameters is based on experimental and production experience, and is intended to ensure that the preparation of the insecticide is both efficient and uniform.

[0060] Preferably, we can further refine these operating conditions. For example, the stirring speed can be preferably set at 400 rpm, which has been proven to provide good mixing effects while avoiding emulsion damage caused by excessive shearing. The stirring time can be preferably set at 45 minutes, which can ensure sufficient mixing of the components and improve production efficiency. In actual operation, the stirring speed and time can be adjusted according to the specific production equipment and the required emulsion properties.

[0061] Furthermore, different agitator types, such as paddle agitators or turbine agitators, can be considered to meet different mixing requirements. Through these refined operating steps, the optimization of the mixing process and the high quality of the final product can be ensured.

[0062] In some embodiments, before the mixing step, the pesticide technical is pretreated and ground to a particle size of less than 10 microns to improve its dispersibility in the compounded emulsifiable concentrate pesticide.

[0063] It should be noted that this embodiment describes the process of pre-treating the original pesticide before the mixing step, the purpose of which is to improve the dispersibility of the original pesticide in the compound emulsifiable concentrate pesticide. Particle size refers to the diameter of the particles and is an important factor affecting dispersibility and uniformity.

[0064] Specifically, before the mixing step, the pesticide technical needs to be pre-treated and ground to a particle size of less than 10 microns. This step is to ensure that the pesticide technical can be evenly dispersed in the emulsifiable concentrate matrix, improve the efficacy of the pesticide and reduce the damage to crops. Particle size control is achieved by using appropriate grinding equipment and process parameters to ensure that the particles of the pesticide technical are small enough to improve its dispersibility in the emulsifiable concentrate matrix.

[0065] Preferably, a ball mill or air flow mill can be used for grinding, which can effectively reduce the particle size of the particles. In actual operation, the particle size of the pesticide original drug can be controlled by adjusting the operating parameters of the equipment, such as the speed and time of the ball mill or the pressure and flow of the air flow mill. Screening equipment can also be used to further grade the particle size of the ground pesticide original drug to ensure that all particles meet the required particle size requirements.

[0066] Example 1 A method for preparing a compound emulsifiable concentrate insecticide is prepared from the following components: 10 parts of pyrethroid technical, 20 parts of organophosphorus technical, 3 parts of pea albumin, 6 parts of Pichia pastoris fermentation product, 50 parts of xylene, 2.4 parts of nonionic emulsifier, and 0.8 parts of anionic emulsifier.

[0067] The preparation method of this embodiment comprises the following steps: S1. inoculating Pichia pastoris into a medium containing a carbon source, a nitrogen source, inorganic salts and trace elements, fermenting and culturing at 30°C and pH 5.5 for 40 hours, then performing solid-liquid separation and collecting the fermentation product; S2. dissolving pea albumin in phosphate buffer solution to prepare a 7% w / v pea albumin solution; S3. Grind the pyrethroid original drug and the organophosphorus original drug to a particle size of less than 10 μm and mix them evenly; S4. The xylene, nonionic emulsifier and anionic emulsifier are mixed in a mass ratio of 3:1 to form an emulsifiable concentrate base; S5. The mixed pesticide original drug in step S3, the pea albumin solution in step S2, the Pichia fermentation product in step S1 and the emulsifiable concentrate base in step S4 are sequentially added to the reactor and stirred at a speed of 400 rpm for 45 minutes to obtain a composite emulsifiable concentrate insecticide; S6. Finally, the obtained emulsifiable concentrate insecticide is cooled to room temperature.

[0068] In this example, the xylene used is of industrial purity, the nonionic emulsifier and the anionic emulsifier are both commercially available products, and the collection of the Pichia yeast fermentation product is carried out under sterile conditions to ensure the biological safety of the product. Experimental data show that the compound emulsifiable concentrate insecticide prepared in this example has a good control effect on a variety of pests.

[0069] Experimental data: Synthesis method: Follow the above steps S1 to S6.

[0070] Reaction conditions: Pichia yeast fermentation culture for 40 hours, pea albumin solution prepared with a pH of 7.0, the particle size of the pesticide original drug is less than 10 microns, the stirring speed is 400 rpm, and the stirring time is 45 minutes.

[0071] Yield: The theoretical yield is 100%, and the actual yield is 97%.

[0072] Purity: The purity of pyrethroid technical and organophosphorus technical is greater than 98% as analyzed by high performance liquid chromatography (HPLC).

[0073] Uses and effects: This compound emulsifiable concentrate insecticide is suitable for controlling a variety of agricultural pests. Experiments have shown that the use of this insecticide significantly reduces the number of farmland pests, reduces crop loss rates, and has less impact on the environment.

[0074] Example 2 A method for preparing a compound emulsifiable concentrate insecticide is prepared from the following components: 8 parts of pyrethroid technical, 16 parts of organophosphorus technical, 4 parts of pea albumin, 7 parts of Pichia pastoris fermentation product, 60 parts of xylene, 2.7 parts of nonionic emulsifier, and 0.9 parts of anionic emulsifier.

[0075] The preparation method of this embodiment comprises the following steps: S1. inoculating Pichia pastoris into a medium containing a carbon source, a nitrogen source, inorganic salts and trace elements, fermenting and culturing at 31°C and pH 5.8 for 48 hours, then performing solid-liquid separation and collecting the fermentation product; S2. The pea albumin was dissolved in a phosphate buffer solution having a pH of 7.2 to prepare an 8% w / v pea albumin solution; S3. Grind the pyrethroid original drug and the organophosphorus original drug to a particle size of less than 10 μm and mix them evenly; S4. The xylene, nonionic emulsifier and anionic emulsifier are mixed in a mass ratio of 3:1 to form an emulsifiable concentrate base; S5. The insecticide original drug mixed evenly in step S3, the pea albumin solution in step S2, the Pichia yeast fermentation product in step S1 and the emulsifiable concentrate base in step S4 are sequentially added to the reactor and stirred at a speed of 450 rpm for 50 minutes to obtain a composite emulsifiable concentrate insecticide; S6. Finally, the obtained emulsifiable concentrate insecticide is cooled to room temperature.

[0076] In this example, the xylene used is of industrial purity, the nonionic emulsifier and the anionic emulsifier are both commercially available products, and the collection of the Pichia fermentation product is carried out under sterile conditions to ensure the biosafety of the product. Experimental data show that the compound emulsifiable concentrate insecticide prepared in this example has a good control effect on a variety of pests, and the ratio of pea albumin to the Pichia fermentation product meets the specific mass fraction requirements.

[0077] Experimental data: Synthesis method: Follow the above steps S1 to S6.

[0078] Reaction conditions: Pichia yeast fermentation culture for 48 hours, pea albumin solution prepared with a pH of 7.2, the particle size of the pesticide original drug is less than 10 microns, the stirring speed is 450 rpm, and the stirring time is 50 minutes.

[0079] Yield: The theoretical yield is 100%, and the actual yield is 96%.

[0080] Purity: The purity of pyrethroid technical and organophosphorus technical is greater than 98% as analyzed by high performance liquid chromatography (HPLC).

[0081] Uses and effects: This compound emulsifiable concentrate insecticide is suitable for controlling a variety of agricultural pests. Experiments have shown that the use of this insecticide significantly reduces the number of farmland pests, reduces crop loss rates, and has less impact on the environment.

[0082] Example 3 A method for preparing a compound emulsifiable concentrate insecticide is prepared from the following components: 12 parts of pyrethroid technical, 24 parts of organophosphorus technical, 2 parts of pea albumin, 5 parts of Pichia pastoris fermentation product, 55 parts of cyclohexanone, 2.1 parts of nonionic emulsifier, and 0.7 parts of anionic emulsifier.

[0083] The preparation method of this embodiment comprises the following steps: S1. inoculating Pichia pastoris into a medium containing a carbon source, a nitrogen source, inorganic salts and trace elements, fermenting and culturing at 29°C and pH 5.2 for 36 hours, then performing solid-liquid separation and collecting the fermentation product; S2. The pea albumin was dissolved in a phosphate buffer solution having a pH of 6.8 to prepare a 6% w / v pea albumin solution; S3. Grind the pyrethroid original drug and the organophosphorus original drug to a particle size of less than 10 μm and mix them evenly; S4. Cyclohexanone, a nonionic emulsifier and an anionic emulsifier are mixed in a mass ratio of 3:1 to form an emulsifiable concentrate base; S5. The mixed insecticide original drug in step S3, the pea albumin solution in step S2, the Pichia fermentation product in step S1 and the emulsifiable concentrate base in step S4 are sequentially added to the reactor and stirred at a speed of 350 rpm for 30 minutes to obtain a composite emulsifiable concentrate insecticide; S6. Finally, the obtained emulsifiable concentrate insecticide is cooled to room temperature.

[0084] In this embodiment, the cyclohexanone used is of industrial purity, the nonionic emulsifier and the anionic emulsifier are both commercially available products, and the collection of the Pichia yeast fermentation product is carried out under sterile conditions to ensure the biological safety of the product. Experimental data show that the compound emulsifiable concentrate insecticide prepared in this embodiment has a good control effect on a variety of pests, and the mass ratio of pyrethroid original drug to organophosphorus original drug is 1:2.

[0085] Experimental data: Synthesis method: Follow the above steps S1 to S6.

[0086] Reaction conditions: Pichia yeast fermentation culture for 36 hours, pea albumin solution prepared with a pH of 6.8, the particle size of the pesticide original drug is less than 10 microns, the stirring speed is 350 rpm, and the stirring time is 30 minutes.

[0087] Yield: The theoretical yield is 100%, and the actual yield is 95%.

[0088] Purity: The purity of pyrethroid technical and organophosphorus technical is greater than 98% as analyzed by high performance liquid chromatography (HPLC).

[0089] Uses and effects: This compound emulsifiable concentrate insecticide is suitable for controlling a variety of agricultural pests. Experiments have shown that the use of this insecticide significantly reduces the number of farmland pests, reduces crop loss rates, and has less impact on the environment.

[0090] Example 4 A method for preparing a compound emulsifiable concentrate insecticide is prepared from the following components: 10 parts of pyrethroid technical, 20 parts of organophosphorus technical, 3 parts of pea albumin, 7 parts of Pichia pastoris fermentation product, 50 parts of toluene, 2.5 parts of nonionic emulsifier, and 0.8 parts of anionic emulsifier.

[0091] The preparation method of this embodiment comprises the following steps: S1. inoculating Pichia pastoris into a medium containing a carbon source, a nitrogen source, inorganic salts and trace elements, fermenting and culturing at 30°C and pH 5.5 for 40 hours, then performing solid-liquid separation and collecting the fermentation product; S2. The pea albumin was dissolved in a phosphate buffer solution having a pH of 7.0 to prepare an 8% w / v pea albumin solution; S3. Grind the pyrethroid original drug and the organophosphorus original drug to a particle size of less than 10 μm and mix them evenly; S4. The toluene, nonionic emulsifier and anionic emulsifier are mixed in a mass ratio of 3:1 to form an emulsifiable concentrate base; S5. The mixed pesticide original drug in step S3, the pea albumin solution in step S2, the Pichia fermentation product in step S1 and the emulsifiable concentrate base in step S4 are sequentially added to the reactor and stirred at a speed of 400 rpm for 45 minutes to obtain a composite emulsifiable concentrate insecticide; S6. Finally, the obtained emulsifiable concentrate insecticide is cooled to room temperature.

[0092] In this embodiment, the toluene used is industrial purity, the nonionic emulsifier and the anionic emulsifier are both commercially available products, and the collection of the Pichia yeast fermentation product is carried out under sterile conditions to ensure the biosafety of the product. Experimental data show that the compound emulsifiable concentrate insecticide prepared in this embodiment has a good control effect on a variety of pests, and the ratio of the solvent to the emulsifier in the emulsifiable concentrate base meets the specific mass ratio requirements.

[0093] Experimental data: Synthesis method: Follow the above steps S1 to S6.

[0094] Reaction conditions: Pichia yeast fermentation culture for 40 hours, pea albumin solution prepared with a pH of 7.0, the particle size of the pesticide original drug is less than 10 microns, the stirring speed is 400 rpm, and the stirring time is 45 minutes.

[0095] Yield: The theoretical yield is 100%, and the actual yield is 97%.

[0096] Purity: The purity of pyrethroid technical and organophosphorus technical is greater than 98% as analyzed by high performance liquid chromatography (HPLC).

[0097] Uses and effects: This compound emulsifiable concentrate insecticide is suitable for controlling a variety of agricultural pests. Experiments have shown that the use of this insecticide significantly reduces the number of farmland pests, reduces crop loss rates, and has less impact on the environment.

[0098] Example 5 A method for preparing a compound emulsifiable concentrate insecticide is prepared from the following components: 8 parts of pyrethroid technical, 16 parts of organophosphorus technical, 4 parts of pea albumin, 8 parts of Pichia pastoris fermentation product, 60 parts of xylene, 3 parts of nonionic emulsifier and 1 part of anionic emulsifier.

[0099] The preparation method of this embodiment comprises the following steps: S1. inoculating Pichia pastoris into a medium containing a carbon source, a nitrogen source, inorganic salts and trace elements, fermenting and culturing at 29°C and pH 5.5 for 40 hours, then performing solid-liquid separation and collecting the fermentation product; S2. The pea albumin was dissolved in a phosphate buffer solution having a pH of 7.0 to prepare a 9% w / v pea albumin solution; S3. Grind the pyrethroid original drug and the organophosphorus original drug to a particle size of less than 10 μm and mix them evenly; S4. The xylene, nonionic emulsifier and anionic emulsifier are mixed in a mass ratio of 3:1 to form an emulsifiable concentrate base; S5. The insecticide original drug mixed evenly in step S3, the pea albumin solution in step S2, the Pichia yeast fermentation product in step S1 and the emulsifiable concentrate base in step S4 are sequentially added to the reactor and stirred at a speed of 450 rpm for 50 minutes to obtain a composite emulsifiable concentrate insecticide; S6. Finally, the obtained emulsifiable concentrate insecticide is cooled to room temperature.

[0100] In this embodiment, the xylene used is of industrial purity, the nonionic emulsifier and the anionic emulsifier are both commercially available products, and the collection of the Pichia yeast fermentation product is carried out under sterile conditions to ensure the biosafety of the product. Experimental data show that the compound emulsifiable concentrate insecticide prepared in this embodiment has a good control effect on a variety of pests, and the ratio of the solvent and the emulsifier in the emulsifiable concentrate matrix meets the specific mass fraction requirements.

[0101] Experimental data: Synthesis method: Follow the above steps S1 to S6.

[0102] Reaction conditions: Pichia yeast fermentation culture for 40 hours, pea albumin solution prepared with a pH of 7.0, the particle size of the pesticide original drug is less than 10 microns, the stirring speed is 450 rpm, and the stirring time is 50 minutes.

[0103] Yield: The theoretical yield is 100%, and the actual yield is 96%.

[0104] Purity: The purity of pyrethroid technical and organophosphorus technical is greater than 98% as analyzed by high performance liquid chromatography (HPLC).

[0105] Uses and effects: This compound emulsifiable concentrate insecticide is suitable for controlling a variety of agricultural pests. Experiments have shown that the use of this insecticide significantly reduces the number of farmland pests, reduces crop loss rates, and has less impact on the environment.

[0106] Comparative Example 1 This comparative example is a traditional emulsifiable concentrate insecticide, which is prepared from the following raw materials in parts by weight: 20 parts of organophosphorus technical, 60 parts of xylene, 2.0 parts of nonionic emulsifier, 0.7 parts of anionic emulsifier; The preparation method of the above-mentioned emulsifiable concentrate insecticide comprises the following steps: (a) mixing an organophosphorus technical drug with an appropriate amount of solvent, stirring to fully dissolve the organic phosphorus technical drug, and then filtering to remove insoluble matter to obtain an organic phosphorus technical drug solution for use; (b) mixing xylene, a nonionic emulsifier and an anionic emulsifier and heating them to 80° C., and after they are completely melted, obtaining a mixed matrix; (c) When the mixed matrix is ​​cooled to 60° C., the organophosphorus original drug solution is added and stirred to obtain the emulsifiable concentrate insecticide.

[0107] Comparative Example 2 This comparative example is a traditional emulsifiable concentrate insecticide, which is prepared from the following raw materials in parts by weight: 25 parts of organophosphorus technical, 65 parts of xylene, 2.2 parts of nonionic emulsifier, 0.8 parts of anionic emulsifier; The preparation method of the above-mentioned emulsifiable concentrate insecticide comprises the following steps: (a) mixing an organophosphorus technical drug with an appropriate amount of solvent, stirring to fully dissolve the organic phosphorus technical drug, and then filtering to remove insoluble matter to obtain an organic phosphorus technical drug solution for use; (b) mixing xylene, a nonionic emulsifier and an anionic emulsifier and heating them to 85° C., and after they are completely melted, obtaining a mixed matrix; (c) When the mixed matrix is ​​cooled to 70° C., the organophosphorus original drug solution is added and stirred to obtain the emulsifiable concentrate insecticide.

[0108] Experimental example The emulsifiable concentrate insecticides prepared in Example 6 and Comparative Examples 1-2 were obtained respectively; The above-mentioned emulsifiable concentrate insecticide is used to control pests in farmland, and the pest types include aphids, cotton bollworms, etc.; wherein, the pesticide of Example 6 has a cumulative farmland area of ​​63 mu, the pesticide of Comparative Example 1 has a cumulative farmland area of ​​41 mu, and the pesticide of Comparative Example 2 has a cumulative farmland area of ​​36 mu; Prevention and treatment methods: Use a sprayer to evenly spray the pesticide on the farmland once every three days; continue spraying for three times, and then observe the changes in the number of pests; Criteria for judging control effects: Advantages: Pest population reduced by more than 90%; Good: Pest population reduced by 60%-89%; Can: reduce the number of pests by 30%-59%; Poor: Pest population reduction of 0-29%.

[0109] The statistical control effects are shown in Tables 1 to 3: Table 1 Statistics of aphid control effects

[0110] Table 2 Statistics on the control effect of cotton bollworm

[0111] Table 3 Statistics of comprehensive control effects

[0112] From Tables 1 to 3, we can see that: The emulsifiable concentrate insecticide prepared by the technical solution of the present application and the comparative example has a certain control effect on different types of pests. Compared with the comparative example, the emulsifiable concentrate insecticide prepared by the technical solution of the present application has a significant control effect on pests such as aphids and cotton bollworms.

[0113] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or replace some or all of the technical features therein by equivalents. These modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of the technical solutions of the embodiments of the present invention, and they should all be included in the scope of the claims and specification of the present invention.

[0114] The above descriptions are only some preferred embodiments of the present invention and an explanation of the technical principles used. Those skilled in the art should understand that the scope of the invention involved in the embodiments of the present invention is not limited to the technical solutions formed by a specific combination of the above technical features, but should also cover other technical solutions formed by any combination of the above technical features or their equivalent features without departing from the above inventive concept. For example, the above features are replaced with (but not limited to) technical features with similar functions disclosed in the embodiments of the present invention.

Claims

1. A compound emulsifiable concentrate insecticide for pest control, characterized in that: The compound emulsifiable concentrate insecticide comprises active ingredients, pea albumin, a Pichia yeast fermentation product and an emulsifiable concentrate base, wherein the active ingredients comprise at least two insecticide original drugs with different action mechanisms; Based on the total mass of the compound emulsifiable concentrate insecticide, the mass fraction of pea albumin is 1%-5%, and the mass fraction of Pichia yeast fermentation product is 3%-8%; The two pesticide technicals with different action mechanisms are pyrethroid technical and organophosphorus technical, and the mass ratio of pyrethroid technical to organophosphorus technical is 1:2; The emulsifiable concentrate base comprises a solvent and an emulsifier, wherein the solvent is selected from at least one of xylene, toluene and cyclohexanone, and the emulsifier is a mixture of a nonionic emulsifier and an anionic emulsifier in a mass ratio of 3:

1.

2. A composite emulsifiable concentrate insecticide for pest control according to claim 1, characterized in that, Based on the total mass of the compound emulsifiable concentrate insecticide, the mass fraction of the solvent is 50%-70%, and the mass fraction of the emulsifier is 8%-15%.

3. A method for preparing a composite emulsifiable concentrate insecticide for pest control according to any one of claims 1 to 2, characterized in that: The preparation method comprises: Cultivating Pichia pastoris: inoculating Pichia pastoris into a culture medium for fermentation to obtain a Pichia pastoris fermentation broth, performing solid-liquid separation on the fermentation broth, and collecting a fermentation product; preparing a pea albumin solution: dissolving pea albumin in a buffer solution to prepare a pea albumin solution; Mixing: adding the original insecticide, pea albumin solution, Pichia yeast fermentation product, and the solvent and emulsifier in the emulsifiable concentrate base into the reaction kettle in proportion, stirring and mixing evenly to obtain the composite emulsifiable concentrate insecticide.

4. A method for preparing a composite emulsifiable concentrate insecticide for pest control according to claim 3, characterized in that, In the step of culturing Pichia pastoris, the nutrients of the culture medium include a carbon source, a nitrogen source, inorganic salts and trace elements, the fermentation temperature is 28° C.-32° C., the pH is 5.0-6.0, and the fermentation time is 36-48 hours.

5. A method for preparing a composite emulsifiable concentrate insecticide for pest control according to claim 4, characterized in that, In the step of preparing the pea albumin solution, the buffer solution is a phosphate buffer solution with a pH of 6.5-7.5, and the concentration of the pea albumin solution is 5%-10% w / v.

6. A method for preparing a composite emulsifiable concentrate insecticide for pest control according to claim 5, characterized in that: In the mixing step, the stirring speed is 300-500 rpm, and the stirring time is 30-60 minutes.

7. A method for preparing a composite emulsifiable concentrate insecticide for pest control according to claim 6, characterized in that: Prior to the mixing step, the pesticide technical is pre-treated and ground to a particle size of less than 10 microns.

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