Application of baicalein in preparation of feed for preventing and treating porcine reproductive and respiratory syndrome

Abstract

The invention discloses application of baicalein in preparation of feed for preventing and treating porcine reproductive and respiratory syndrome, and belongs to the technical field of feed. The baicalein is applied to preparation of feed for preventing and treating the porcine reproductive and respiratory syndrome. The baicalein is applied to the feed, and the baicalein in the feed can inhibit the replication function of PRRSV causing the porcine reproductive and respiratory syndrome, so that the incidence rate of the porcine reproductive and respiratory syndrome is reduced, the infectious disease of pigs suffering from the porcine reproductive and respiratory syndrome is relieved, and the disease course is shortened.

Description

Technical Field

[0001] The invention relates to the technical field of feed, and in particular to application of baicalein in preparing feed for preventing and treating blue ear disease. Background Art

[0002] Porcine reproductive and respiratory syndrome virus (PRRSV) is a highly contagious viral disease whose target cells are mainly alveolar macrophages. PRRSV infection can cause reproductive disorders (abortion), respiratory symptoms, secondary infection, immunosuppression, lung damage and other symptoms in pigs, commonly known as "blue ear disease". In addition to causing death and health damage to pigs during the acute outbreak period, blue ear disease positivity (latent period) can also cause slow growth of pigs and a significant increase in feed-to-meat ratio, causing huge economic losses to the pig industry.

[0003] Existing technologies usually use veterinary methods such as purification and vaccination to prevent and control blue ear disease, but there is currently a lack of specific antiviral drugs for PRRSV in clinical practice (commonly used antibiotics only control secondary infections). In addition, due to the rapid mutation of PRRSV, immune escape often occurs after vaccination, resulting in a low protection rate of existing vaccines, which is only about 50-70%. Summary of the invention

[0004] In view of this, the purpose of the present invention is to provide the use of baicalein in the preparation of feed for preventing and treating blue ear disease, so as to overcome the technical problem that the prior art has poor prevention and treatment effect on blue ear disease in pigs.

[0005] In a first aspect, the present invention provides the use of baicalein in preparing feed for preventing and treating blue ear disease.

[0006] Compared with the prior art, the present invention uses baicalin to prepare feed. After baicalin is added to the feed, baicalin can inhibit the replication function of PRRSV that causes blue ear disease in pigs, thereby reducing the incidence of blue ear disease and alleviating the infection symptoms of blue ear disease pigs. At the same time, baicalin also has a strong antioxidant capacity, which helps to clear the oxidative stress products generated during the virus infection process and shorten the course of the disease.

[0007] Furthermore, based on the mass of the feed of each kilogram, the effective amount of baicalin for preventing and treating blue ear disease is 0.1 to 0.5 g.

[0008] The above technical scheme further limits the effective amount of baicalin for preventing and treating blue ear disease in feed. Within the above effective amount range, baicalin can better play a preventive and therapeutic role on blue ear disease in pigs, further reduce the incidence of blue ear disease, alleviate symptoms, and shorten the course of the disease.

[0009] Furthermore, baicalein is used in the form of a composition, which contains baicalein and low-protein whey powder in an effective amount for preventing and treating blue ear disease.

[0010] The above technical scheme further limits the addition of baicalein and low-protein whey powder in the form of a composition to feed for preventing and treating blue ear disease. The cooperation between baicalein and low-protein whey powder can improve the intestinal mucosal development and microecological environment of blue ear disease pigs, promote the absorption and transport of nutrients, and thus improve the health level and growth and development of pigs.

[0011] Furthermore, based on the mass of the feed per kilogram, in the composition, the amount of baicalin added in an effective amount for preventing and treating blue ear disease is 0.1 to 0.5 g, and the amount of low-protein whey powder added is 50 to 100 g.

[0012] The above technical scheme further limits the addition amount of baicalein and low-protein whey powder in the composition. Within the above addition amount range, the synergistic effect of baicalein and low-protein whey powder is further improved, the intestinal health of pigs is further improved, and the growth and development of pigs is promoted.

[0013] Furthermore, the composition further comprises choline chloride or bile acid; and the added amount of the choline chloride or bile acid in the composition is 2 to 4 g per kilogram of the mass of the feed.

[0014] The above technical solution further limits that the composition can also contain choline chloride or bile acid, and baicalein cooperates with choline chloride or bile acid to promote fat transport and improve liver health.

[0015] In a second aspect, the present invention provides a feed for preventing and treating blue ear disease, comprising a basic feed and baicalin for preventing and treating blue ear disease.

[0016] Compared with the prior art, the present invention adds baicalin which has a preventive and therapeutic effect on blue ear disease in pigs to the feed, which can improve the immunity of pigs and prevent pigs from being infected with PRRSV. For pigs that have been infected with blue ear disease of PRRSV, the invention can alleviate the infection symptoms, shorten the course of the disease and promote the recovery of sick pigs.

[0017] Furthermore, baicalein is used in the form of a composition comprising baicalein and low-protein whey powder.

[0018] The above technical scheme adds baicalein and low-protein whey powder in the form of a composition to feed for preventing and treating blue ear disease. Baicalein and low-protein whey powder work synergistically with each other, which can significantly improve the intestinal health of blue ear disease piglets, promote the digestion and absorption of various nutrients in the feed, and significantly improve the growth performance of weaned piglets.

[0019] Furthermore, the composition further comprises choline chloride or bile acid.

[0020] The above technical scheme further stipulates that the feed for preventing and treating blue ear disease also contains choline chloride or bile acid, which, when combined with baicalein, can promote fat transport and improve liver health.

[0021] Furthermore, the basic feed includes the following components: corn, protein substances, oils, sugars, complex amino acids, feed additives, enzyme preparations and premixes.

[0022] The above technical solution defines the composition of the basic feed, which can provide rich nutrients for pigs and promote the growth and development of pigs.

[0023] Furthermore, the feed contains the following components, measured by mass per kilogram of feed: 500-680g of corn, 250-350g of protein, 15-35g of oil, 20-50g of sugar, 5-15g of complex amino acids, 10-30g of feed additives, 0.1-0.5g of enzyme preparation, 10-30g of premix, 0.1-0.5g of baicalein and 50-100g of low-protein whey powder.

[0024] The above technical solution further limits the content range of each component of the feed. Within the above content range, each nutrient is reasonably proportioned and evenly distributed, which can enable the healthy growth of pigs. In addition, the above specific proportion of baicalein and low-protein whey powder can significantly improve the intestinal health of blue ear disease piglets.

[0025] Furthermore, the feed further comprises 2-4 g of choline chloride or 2-4 g of bile acid per kilogram of the feed.

[0026] The above technical solution further limits the feed to also contain a certain amount of choline chloride or bile acid, which acts synergistically with baicalein to promote fat transport and nutrient absorption and improve liver health. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 This is a diagram showing lung damage in piglets infected with blue ear virus in Test Example 2.

[0028] Figure 2 This is the apparent pathological score of the lung in Test Example 2.

[0029] Figure 3 is the expression level of viral mRNA in the lungs in Test Example 2.

[0030] Figure 4 It is the content of immunoglobulin G (IgG), interferon β (IFN-β), and interferon γ (IFN-γ) in the blood of piglets infected with blue ear virus in test example 3.

[0031] Figure 5 It is the serum catalase (CAT) content in piglets infected with blue ear virus in test example 4.

[0032] Figure 6It is the content of liver glutathione peroxidase (GSH-PX) in piglets infected with blue ear virus in test example 4.

[0033] Figure 7 This is a diagram of the development of the small intestinal mucosa of piglets infected with blue ear virus in Test Example 5.

[0034] Figure 8 This is the microecological environment of the small intestine of piglets infected with blue ear virus in Test Example 5.

[0035] Fig. 9 It is the content of jejunal lipase and α-amylase in piglets infected with blue ear virus in test example 6.

[0036] Fig.10 This is a graph showing the inhibitory effect of baicalin on PRRSV under different treatments in Test Example 7.

[0037] Fig.11 The killing effect of baicalein at different concentrations on PRRSV in Test Example 7 was tested.

[0038] Fig.12 This is a graph showing the binding ability of baicalein to PRRSV envelope protein GP5 / M in Test Example 7. DETAILED DESCRIPTION

[0039] In order to make the technical problems, technical solutions and beneficial effects to be solved by the present invention more clearly understood, the present invention is further described in detail below in conjunction with specific embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0040] In a first aspect, an embodiment of the present invention provides the use of baicalein in preparing feed for preventing and treating blue ear disease.

[0041] The baicalin in the above technical solution has a direct killing effect on PRRSV, which is achieved through stable binding to the PRRSV envelope protein GP5 / M. Therefore, feed added with baicalin can effectively prevent and treat blue ear disease in pigs, reduce the incidence of blue ear disease, and alleviate the infection symptoms of blue ear disease-sick pigs.

[0042] Furthermore, based on the above embodiment, the embodiment of the present invention further selects that the effective amount of baicalin for preventing and treating blue ear disease is 0.1-0.5g per kilogram of the mass of the feed. For example, the effective amount of baicalin for preventing and treating blue ear disease can be 0.1g, 0.2g, 0.3g, 0.4g or 0.5g, preferably 0.1-0.3g. Baicalin within the above effective amount range has the best inhibitory effect on PRRSV.

[0043] Furthermore, on the basis of the above embodiment, the embodiment of the present invention further selects baicalin to be used in the form of a composition, and the composition comprises baicalin and low-protein whey powder in an effective amount for preventing and treating blue ear disease. In the above composition, the amount of baicalin added in an effective amount for preventing and treating blue ear disease is 0.1-0.5 g, and the amount of low-protein whey powder added is 50-100 g per kilogram of the mass of the feed.

[0044] For example, the amount of baicalin added for the effective prevention and treatment of blue ear disease can be 0.1g, 0.2g, 0.3g, 0.4 or 0.5g, and the amount of low-protein whey powder added can be 50g, 60g, 70g, 80g, 90g or 100g, preferably 50-70g.

[0045] The baicalein and low-protein whey powder in the above technical solution have a synergistic effect, which can significantly improve the intestinal mucosal development and microecological environment of blue ear disease pigs, promote the absorption and transportation of nutrients, and thus improve the health level of piglets.

[0046] Further, based on the above embodiment, the present invention further selects that the composition further comprises choline chloride or bile acid; in the composition, the amount of choline chloride or bile acid added is 2 to 4 g per kilogram of the mass of the feed. For example, the amount of choline chloride or bile acid added can be 2 g, 3 g or 4 g.

[0047] In the above technical solution, choline chloride or bile acid is additionally added to the composition, which cooperates with baicalein to promote fat transport and improve liver health.

[0048] In a second aspect, an embodiment of the present invention provides a feed for preventing and treating blue ear disease, comprising a basic feed and baicalein for preventing and treating blue ear disease.

[0049] Furthermore, baicalein is used in the form of a composition, which comprises baicalein and low-protein whey powder. Furthermore, the composition further comprises choline chloride or bile acid.

[0050] On the basis of the above embodiments, the embodiments of the present invention further select the basic feed to include the following components: corn, protein substances, oils, sugars, complex amino acids, feed additives, enzyme preparations and premixes.

[0051] Furthermore, the feed contains the following components, measured by weight per kilogram of the feed: 500-680 g of corn, 250-350 g of protein, 15-35 g of oil, 20-50 g of sugar, 5-15 g of complex amino acids, 10-30 g of feed additives, 0.1-0.5 g of enzyme preparation, 10-30 g of premix, 0.1-0.5 g of baicalein and 50-100 g of low-protein whey powder.

[0052] For example, corn can be 500g, 550g, 600g, 640g or 680g; protein substances can be 250g, 300g or 350g, oils can be 15g, 20g, 25g, 30g or 35g; sugars can be 20g, 30g, 40g or 50g; complex amino acids can be 5g, 10g or 15g; feed additives can be 10g, 20g or 30g; enzyme preparations can be 0.1g, 0.2g, 0.3g, 0.4g or 0.5g; premix can be 10g, 20g or 30g; baicalein can be 0.1g, 0.2g, 0.3g, 0.4g or 0.5g; low protein whey powder can be 50g, 60g, 70g, 80g, 90g or 100g.

[0053] Furthermore, the feed further comprises 2-4 g of choline chloride or 2-4 g of bile acid per kilogram of the feed. For example, the content of choline chloride or bile acid in the feed may be 2 g, 3 g or 4 g.

[0054] In some embodiments, the protein material can be selected from soybean meal, whey protein concentrate and fish meal, and the mass ratio of the three is (8-9): 1: 1. The oil can be selected from soybean oil. The sugar can be selected from sucrose.

[0055] In some embodiments, the composite amino acids can be selected from lysine, methionine and threonine, and the mass ratio of the three is (3.2-3.8):(1-1.5):(0.8-1.2); among which lysine is preferably lysine hydrochloride, methionine is preferably DL-methionine, and threonine is preferably L-threonine.

[0056] In some embodiments, the feed additives can be selected from calcium hydrogen phosphate, calcium citrate, stone powder, sodium chloride, zinc oxide and titanium dioxide, and their mass ratio is (7-9): (4-6): (4-6): (3-4): (1.5-2): (3.5-4.5).

[0057] In some embodiments, the enzyme preparation may be selected as phytase.

[0058] In some embodiments, the premix provides the following components per kilogram of feed, based on the mass of per kilogram of feed: 9000 IU of vitamin A, 900 IU of vitamin D, 150 mg of vitamin E, 1.15 mg of thiamine, 3.48 mg of riboflavin, 31.4 mg of niacin, 5.3 mg of pyridoxine, 18 g of vitamin B12, 0.06 mg of biotin, 10.4 mg of pantothenic acid, 0.31 mg of folic acid, 95 mg of zinc, 105 mg of iron, 5.8 mg of copper, 3.69 mg of manganese, 0.15 mg of iodine, and 0.29 mg of selenium.

[0059] It should be understood that the raw materials used in the following examples are all commercially available raw materials unless otherwise specified.

[0060] Example 1

[0061] An embodiment of the present invention provides a feed for preventing and treating blue ear disease, comprising the following components: 546.6 g corn, 280 g soybean meal, 35 g whey protein concentrate, 35 g fish meal, 25 g soybean oil, 35 g sucrose, 3.5 g lysine hydrochloride, 1.3 g DL-methionine, 1 g L-threonine, 8 g calcium hydrogen phosphate, 5 g calcium citrate, 5 g stone powder, 3.5 g sodium chloride, 1.8 g zinc oxide, 4 g titanium dioxide, 0.2 g phytase, 10 g premix, and 0.1 g baicalein.

[0062] Example 2

[0063] The embodiment of the present invention provides a feed for preventing and treating blue ear disease, comprising the following components: 538.6g corn, 251g soybean meal, 30g whey protein concentrate, 30g fish meal, 25g soybean oil, 20g sucrose, 3.5g lysine hydrochloride, 1.3g DL-methionine, 1g L-threonine, 8g calcium hydrogen phosphate, 5g calcium citrate, 5g stone powder, 3.5g sodium chloride, 1.8g zinc oxide, 4g titanium dioxide, 0.1g phytase, 10g premix, 0.2g baicalein, 60g low-protein whey powder, and 2g choline chloride.

[0064] Example 3

[0065] An embodiment of the present invention provides a feed for preventing and treating blue ear disease, comprising the following components: 520.3g corn, 251g soybean meal, 30g whey protein concentrate, 30g fish meal, 35g soybean oil, 20g sucrose, 3.5g lysine hydrochloride, 1.3g DL-methionine, 1g L-threonine, 8g calcium hydrogen phosphate, 5g calcium citrate, 5g stone powder, 3.5g sodium chloride, 1.8g zinc oxide, 4g titanium dioxide, 0.3g phytase, 10g premix, 0.3g baicalein and 70g low-protein whey powder.

[0066] Example 4

[0067] The embodiment of the present invention provides a feed for preventing and treating blue ear disease, comprising the following components: 502.1g corn, 280g soybean meal, 35g whey protein concentrate, 35g fish meal, 30g soybean oil, 20g sucrose, 3.5g lysine hydrochloride, 1.3g DL-methionine, 1g L-threonine, 8g calcium hydrogen phosphate, 5g calcium citrate, 5g stone powder, 3.5g sodium chloride, 1.8g zinc oxide, 4g titanium dioxide, 0.4g phytase, 10g premix, 0.4g baicalein, 50g low-protein whey powder, and 4g choline chloride.

[0068] Example 5

[0069] The embodiment of the present invention provides a feed for preventing and treating blue ear disease, comprising the following components: 524.4g corn, 200g soybean meal, 25g whey protein concentrate, 25g fish meal, 15g soybean oil, 50g sucrose, 9g lysine hydrochloride, 3.5g DL-methionine, 2.5g L-threonine, 3.5g calcium hydrogen phosphate, 2g calcium citrate, 2g stone powder, 1.5g sodium chloride, 0.8g zinc oxide, 1.8g titanium dioxide, 0.5g phytase, 30g premix, 0.5g baicalein, 100g low-protein whey powder, and 3g bile acid.

[0070] Comparative Example 1

[0071] The only difference from the feed for preventing and treating blue ear disease provided in Example 1 is that an equal amount of baicalin is replaced by ribavirin.

[0072] Comparative Example 2

[0073] The only difference from the feed for preventing and treating blue ear disease provided in Example 1 is that the content of baicalin in the feed is set to 1 g.

[0074] Comparative Example 3

[0075] The only difference from the feed for preventing and treating blue ear disease provided in Example 3 is that an equal amount of baicalein is replaced by low-protein whey powder.

[0076] Comparative Example 4

[0077] The only difference from the feed for preventing and treating blue ear disease provided in Example 3 is that an equal amount of low-protein whey powder is replaced by baicalein.

[0078] Comparative Example 5

[0079] The only difference from the feed for preventing and treating blue ear disease provided in Example 2 is that an equal amount of baicalein is replaced by choline chloride.

[0080] Comparative Example 6

[0081] The only difference from the feed for preventing and treating blue ear disease provided in Example 5 is that an equal amount of baicalein is replaced by bile acid.

[0082] Test Example 1

[0083] Detection of the effect of baicalin in weaned pig feed

[0084] 110 weaned piglets born 21 days ago were selected and infected with porcine reproductive and respiratory syndrome virus (PRRSV) and then divided into 11 groups. They were fed with the feeds in Examples 1 to 5 and Comparative Examples 1 to 6, 4 times a day, and 2.4 mg / kg each time. After 21 days of feeding, the growth performance of the blue ear virus infected piglets in each group was tested, and the results are shown in Table 1.

[0085] Table 1

[0086] Average daily weight gain (g / d) Average daily feed intake (g / d) Material weight ratio Example 1 461.11 669.57 1.45 Example 2 482.24 689.53 1.43 Example 3 470.87 678.42 1.44 Example 4 484.39 690.36 1.43 Example 5 485.65 691.68 1.42 Comparative Example 1 411.17 604.97 1.47 Comparative Example 2 415.96 619.47 1.49 Comparative Example 3 415.51 610.03 1.47 Comparative Example 4 408.94 604.75 1.48 Comparative Example 5 379.31 563.45 1.49 Comparative Example 6 406.78 615.43 1.51

[0087] It can be seen from Table 1 that in Comparative Example 1, after an equal amount of baicalin was replaced with the antibiotic ribavirin, the average daily weight gain and the average daily feed intake both decreased, and the feed-to-weight ratio increased, indicating that the baicalin in the feed of the present invention has the effect of inhibiting the replication of PRRSV, thereby improving the growth performance of piglets infected with blue ear virus.

[0088] In Comparative Example 2, the amount of baicalein added was increased, and after the baicalein content in the feed was set to 1 g, the average daily weight gain and the average daily feed intake both decreased, and the feed-to-weight ratio increased, indicating that baicalein within a specific content range of the present invention has the best inhibitory effect on PRRSV, thereby improving the growth performance of piglets infected with blue ear virus.

[0089] After replacing an equal amount of baicalein with low-protein whey powder in Comparative Example 3 and replacing an equal amount of low-protein whey powder with baicalein in Comparative Example 4, the average daily weight gain and the average daily feed intake both decreased, and the feed-to-weight ratio increased, indicating that baicalein and low-protein whey powder in the present invention have a synergistic effect, and the cooperation between the two can promote the absorption of nutrients and further improve the growth performance of piglets infected with blue ear virus.

[0090] After replacing an equal amount of baicalein with choline chloride in Comparative Example 5 and replacing an equal amount of baicalein with bile acid in Comparative Example 6, the average daily weight gain and the average daily feed intake both decreased, and the feed-to-weight ratio increased, indicating that the baicalein in the present invention cooperates with choline chloride or bile acid to promote fat transport, improve liver health, and thus further improve the growth performance of piglets infected with blue ear virus.

[0091] Test Example 2

[0092] The lung lesions, lung pathological scores and lung virus mRNA expression levels of the above-mentioned Group 1 (PRRS+SF group, fed with the feed in Example 1) and Group 6 (PRRS group, fed with the feed in Comparative Example 1) blue ear virus-infected piglets were detected. The test results are as follows: Figure 1 , Figure 2 , Figure 3 shown.

[0093] Depend on Figure 1 It can be seen that compared with the PRRS group without baicalin, the degree of lung damage in the PRRS+SF group containing baicalin was lower, indicating that feeding feed containing baicalin can alleviate the lung damage caused by PRRSV.

[0094] Depend on Figure 2It can be seen that compared with the PRRS group without baicalin, the PRRS+SF group containing baicalin reduced the apparent pathological score of the lung.

[0095] Depend on Figure 3 It can be seen that compared with the PRRS group without baicalin, the expression level of viral mRNA in the lungs of the PRRS+SF group containing baicalin was lower, indicating that feeding feed containing baicalin can reduce the expression of porcine blue ear virus mRNA.

[0096] Test Example 3

[0097] The levels of immunoglobulin G (IgG), interferon β (IFN-β), and interferon γ (IFN-γ) in the blood of the above-mentioned group 1 (fed with the feed in Example 1) and group 6 (fed with the feed in Comparative Example 1) infected with blue ear virus were detected. The results are as follows Figure 4 shown.

[0098] Depend on Figure 4 It can be seen that compared with the PRRS group without baicalin, the levels of immunoglobulin G (IgG), interferon β (IFN-β) and interferon γ (IFN-γ) in the PRRS+SF group containing baicalin were higher, indicating that feeding feed containing baicalin can enhance the immunity of piglets infected with blue ear virus and enhance their antiviral ability.

[0099] Test Example 4

[0100] The serum catalase (CAT) content and liver glutathione peroxidase (GSH-PX) content in the blue ear virus infected piglets of the above group 1 (fed with the feed in Example 1) and group 6 (fed with the feed in Comparative Example 1) were detected. The results are as follows: Figure 5 , Figure 6 shown.

[0101] Depend on Figure 5 , Figure 6 It can be seen that compared with the PRRS group without baicalin, the PRRS+SF group containing baicalin had increased catalase content and liver glutathione peroxidase content, indicating that feeding feed containing baicalin can improve the antioxidant capacity of piglets infected with blue ear virus.

[0102] Test Example 5

[0103] The development of the small intestinal mucosa and the microecological environment of the blue ear virus infected piglets in Group 1 (fed with the feed in Example 1) and Group 6 (fed with the feed in Comparative Example 1) were tested. The results are as follows: Figure 7 , Figure 8 shown.

[0104] Depend on Figure 7It can be seen that compared with the PRRS group without baicalin, the small intestinal mucosa of the PRRS+SF group containing baicalin was better developed, indicating that feeding feed containing baicalin can improve the morphology of small intestinal mucosa, enhance resistance to pathogens, and promote the absorption and transportation of nutrients.

[0105] Depend on Figure 8 It can be seen that compared with the PRRS group without baicalin, the abundance of Lactobacillus in the PRRS+SF group containing baicalin was significantly increased, and the abundance of Escherichia was significantly decreased, indicating that feeding feed containing baicalin can improve the microecological environment of the small intestine.

[0106] Test Example 6

[0107] The contents of lipase and α-amylase in the jejunum of piglets infected with blue ear virus in Group 1 (fed with the feed in Example 1) and Group 6 (fed with the feed in Comparative Example 1) were measured. The results are as follows: Fig. 9 shown.

[0108] Depend on Fig. 9 It can be seen that compared with the PRRS group without baicalin, the PRRS+SF group containing baicalin had increased levels of jejunal lipase and α-amylase, indicating that feeding feed containing baicalin can improve the efficiency of nutrient absorption and transportation.

[0109] Test Example 7

[0110] Testing the antiviral mechanism of baicalin in vitro

[0111] 1. MARC-145 cells were pretreated, co-treated and post-treated with baicalin in vitro as follows:

[0112] Pretreatment: The cells were pre-incubated with baicalein at a concentration of 40ug / ml at 37°C for 6h, 12h, and 24h, then rinsed with PBS buffer for 3 times, and then infected with PRRSV for 2h, and the virus titer was detected. The results are as follows: Fig.10 (Preprocessing) as shown.

[0113] Co-treatment: Incubate cells with PRRSV and baicalein at a concentration of 40ug / ml at 37°C for 2h. After removing the PRRSV-baicalein mixture, wash the cells with PBS buffer and detect the virus titer. The results are as follows: Fig.10 (Total Processing) shown.

[0114] Post-treatment: The cells were infected with PRRSV at 37°C for 2 h, and then divided into 3 groups and incubated with 40ug / ml baicalein for 6 h, 12 h, and 24 h, respectively. Samples were collected and the virus titer was detected. The results are as follows: Fig.10 (Post-processing) shown.

[0115] Depend on Fig.10 It can be seen that baicalin pretreatment (left), co-treatment (middle) or post-treatment (right) of MARC-145 cells can effectively inhibit the replication of porcine reproductive and respiratory syndrome virus (PRRSV).

[0116] 2. PRRSV at a dose of 100TCID50 was mixed with different concentrations of baicalin (20, 40, 80ug / ml) and cultured at 37°C for 1 hour. Ultrafiltration centrifugation was used, using an ultrafiltration centrifuge tube containing an ultrafiltration membrane with a pore size of 0.22um, and centrifuged at 7500×g and 4°C for 10 minutes to separate baicalin and PRRSV. The supernatant obtained after ultrafiltration centrifugation was repeatedly ultrafiltered and centrifuged and washed. The supernatant was used to infect MARC-145 cells and the virus titer was detected. The results are as follows: Fig.11 shown.

[0117] Depend on Fig.11 It can be seen that different concentrations of baicalin have a direct killing effect on PRRSV.

[0118] 3. Detect the root mean square deviation and hydrogen bond number of baicalin-GP5 / M complex to determine the binding ability of baicalin to GP5 / M. The results are as follows: Fig.12 shown.

[0119] Depend on Fig.12 It can be seen that baicalein can stably bind to the PRRSV envelope protein GP5 / M, thereby achieving the effect of extracellular killing of PRRSV.

[0120] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention is described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or replace some of the technical features therein by equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. Application of baicalin in the preparation of feed for preventing and treating blue ear disease.

2. The use according to claim 1, characterized in that: Calculated per kilogram of the mass of the feed, the effective amount of baicalin for preventing and treating blue ear disease is 0.1 to 0.5 g.

3. The use according to claim 1 or 2, characterized in that: The baicalein is used in the form of a composition, which contains baicalein and low-protein whey powder in an effective amount for preventing and treating blue ear disease.

4. The use according to claim 3, characterized in that: Calculated per kilogram of the mass of the feed, in the composition, the amount of baicalin added as an effective amount for preventing and treating blue ear disease is 0.1 to 0.5 g, and the amount of low-protein whey powder added is 50 to 100 g.

5. The use according to claim 3, characterized in that: The composition further comprises choline chloride or bile acid; based on the mass of the feed, the added amount of the choline chloride or bile acid in the composition is 2-4 g.

6. A feed for preventing and treating blue ear disease, characterized in that: Includes basic feed and baicalin for preventing and treating blue ear disease.

7. The feed according to claim 6, characterized in that The baicalein is used in the form of a composition, which comprises baicalein and low-protein whey powder.

8. The feed according to claim 7, characterized in that The composition further comprises choline chloride or bile acid.

9. The feed according to any one of claims 6 to 8, characterized in that: The basic feed comprises the following components: corn, protein substances, oils, sugars, complex amino acids, feed additives, enzyme preparations and premixes.

10. The feed according to claim 8, characterized in that The feed comprises the following components per kilogram of the feed: 500-680 g of corn, 250-350 g of protein, 15-35 g of oil, 20-50 g of sugar, 5-15 g of complex amino acid, 10-30 g of feed additives, 0.1-0.5 g of enzyme preparation, 10-30 g of premix, 0.1-0.5 g of baicalein and 50-100 g of low-protein whey powder.

11. The feed according to claim 10, characterized in that The feed further comprises 2-4 g of choline chloride or 2-4 g of bile acid per kilogram of the feed.

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