Composition with weight gaining effect as well as preparation method and application thereof

Abstract

The invention relates to the technical field of composition preparation, and provides a composition with a weight gaining effect and a preparation method and application thereof.Chinese yam, fructus lycii, lotus seeds, fructus crataegi, hericium erinaceus, protein powder, peptide powder, compound probiotic powder and vitamins serve as raw materials, the extraction efficiency of active ingredients in plant raw materials can be improved by optimizing the extraction process of the plant raw materials, and the weight gaining effect is achieved; the formed composition is safe in components and good in stability and has a good weight gaining effect.

Description

Technical Field

[0001] The invention relates to the technical field of composition preparation, and in particular to a composition with a fattening effect, a preparation method and application thereof. Background Art

[0002] Thin people generally suffer from long-term malnutrition and low immunity, and are a high-risk group for autoimmune diseases. Moreover, because thin people have reduced gastrointestinal function and reduced secretion of digestive juices, they eat less, which forms a vicious cycle in the long run. In severe cases, they will develop anemia, hypoproteinemia and other symptoms. Some fattening products and preparation methods thereof are disclosed in the prior art. For example, patent CN103859118A discloses a fattening tea, which is composed of sweet tea, wolfberry, polygonatum, lotus seed, lily, salvia miltiorrhiza, coix seed, adenophora, jujube, malt, hawthorn and spiny jujube kernel, and can consolidate and improve sleep, promote digestion, and achieve healthy and rapid fattening in a short period of time. However, the product is taken in the form of tea, and it is difficult to give full play to the effects of various ingredients; patent CN105343685A discloses a fattening capsule, which is composed of lotus seed, mulberry, Panax notoginseng, salvia miltiorrhiza, Millettia reticulata, Poria cocos, coix seed, black sesame, persimmon calyx, malt, cypress seed, polygala, lily, long-spiked fishtail palm root, white grass flower, wet worm, water hyacinth, Chinese peach leaf, and sword flower stem. Through the compatibility of various medicines, it can nourish the liver and kidney, eliminate symptoms of weakness, and has a good therapeutic effect on emaciation. However, the formula components contain multiple medicinal ingredients and are not suitable for long-term use. Summary of the invention

[0003] In order to solve the above technical problems, the present invention provides a composition with a fattening effect, a preparation method and an application thereof. The composition takes food raw materials as the main ingredients, and the extraction efficiency of active ingredients in the plant raw materials can be improved by optimizing the extraction process of the plant raw materials. The plant extract is then compounded with raw materials such as protein powder, peptide powder and composite probiotic powder. The obtained composition has safe ingredients, good stability, and good fattening effect.

[0004] To achieve the above objectives, the first aspect of the present invention provides a composition with a fattening effect, comprising yam, wolfberry, lotus seeds, hawthorn, hericium erinaceus, protein powder, peptide powder, composite probiotic powder and vitamins, wherein the protein powder is at least one of concentrated whey protein powder, isolated whey protein powder, casein powder and soy protein powder, the peptide powder is at least one of bovine spleen peptide powder, yak bone oligopeptide powder, bone collagen peptide powder, corn peptide powder and cod collagen peptide powder, the composite probiotic powder is at least one of plant lactobacillus, Lactococcus lactis lactic acid subsp. lactis, Streptococcus thermophilus, Lactobacillus rhamnosus and Bifidobacterium animalis lactic acid subsp. lactis, and the vitamins are at least two of vitamin A, vitamin B1, vitamin B2, vitamin B6, vitamin D, vitamin E and vitamin K.

[0005] As a further improvement of the above scheme, the composition further comprises an excipient, a sweetener and a flavoring agent, wherein the excipient is at least one of lactose, maltodextrin, cyclodextrin and microcrystalline cellulose, the sweetener is at least one of xylitol, mannitol, maltitol, oligomaltose, sorbitol and sucrose, and the flavoring agent is at least one of citric acid, malic acid, tartaric acid and taurine.

[0006] The second aspect of the present invention provides a method for preparing the composition with fattening effect according to the first aspect, comprising the following steps:

[0007] S1: crushing and drying yam, wolfberry, lotus seeds, hawthorn, and hericium erinaceus to obtain plant raw material powder;

[0008] S2: using water as an extractant, mixing the plant raw material powder with the extractant and performing ultrasonic-microwave synergistic extraction to obtain a plant extract;

[0009] S3: using the plant extract as an extractant, adding the plant raw material powder in step S1 to perform repeated extraction according to step S2 to obtain a plant multiple extraction solution;

[0010] S4: mixing the plant complex extract with excipients, sweeteners and flavoring agents, stirring and drying to obtain a spray-dried powder;

[0011] S5: stirring and blending the spray-dried powder with protein powder, peptide powder, composite probiotic powder and vitamins to obtain a composition.

[0012] As a further improvement of the above scheme, in step S1, by weight, yam is 20-50 parts, wolfberry is 10-30 parts, lotus seeds are 10-30 parts, hawthorn is 5-15 parts, and Hericium erinaceus is 10-30 parts.

[0013] As a further improvement of the above scheme, in step S4, by weight, the plant complex extract is 70-90 parts, the excipient is 5-20 parts, the sweetener is 1-5 parts, and the flavoring agent is 1-5 parts.

[0014] As a further improvement of the above scheme, in step S4, the excipients consist of maltodextrin and microcrystalline cellulose, the sweetener consists of xylitol, and the flavoring agent consists of citric acid and taurine.

[0015] As a further improvement of the above scheme, in step S5, by weight, the spray-dried powder is 30-60 parts, the protein powder is 10-30 parts, the peptide powder is 10-20 parts, the composite probiotic powder is 5-10 parts, and the vitamins are 0.1-3 parts.

[0016] As a further improvement of the above scheme, the protein powder in step S5 consists of concentrated whey protein powder and isolated whey protein powder, the peptide powder consists of bone collagen peptide powder and cod collagen peptide powder, the composite probiotic powder consists of Lactobacillus rhamnosus and Bifidobacterium animalis subspecies lactis, and the vitamins consist of vitamin B2, vitamin B6 and vitamin D.

[0017] As a further improvement of the above scheme, the preparation method of the composition comprises the following steps:

[0018] S1: According to weight, 40 parts of yam, 15 parts of wolfberry, 10 parts of lotus seeds, 10 parts of hawthorn and 15 parts of Hericium erinaceus were crushed and dried to obtain plant raw material powder;

[0019] S2: Using ultrapure water as an extractant, the plant raw material powder and the extractant are mixed at a solid-liquid ratio of 1 g:5-80 mL, and then subjected to ultrasonic-microwave synergistic extraction, with an ultrasonic power of 100-600 W, a microwave power of 50-800 W, and an extraction time of 1-60 min to obtain a plant extract;

[0020] S3: using the plant extract obtained in step S2 as an extractant, adding the plant raw material powder in step S1 to perform repeated extraction according to step S2 to obtain a plant multiple extraction solution;

[0021] S4: Mix 78 parts of the plant extract with 5 parts of maltodextrin, 10 parts of microcrystalline cellulose, 3 parts of xylitol, 2 parts of citric acid and 2 parts of taurine by weight, stir and dry to obtain a spray-dried powder;

[0022] S5: By weight, 50 parts of spray-dried powder, 10 parts of concentrated whey protein powder, 15 parts of isolated whey protein powder, 10 parts of bone collagen peptide powder, 5 parts of cod collagen peptide powder, 5 parts of Lactobacillus rhamnosus, 4 parts of Bifidobacterium animalis subsp. lactis, 0.2 parts of vitamin B2, 0.3 parts of vitamin B6, and 0.5 parts of vitamin D are stirred and blended to obtain a composition.

[0023] As a further improvement of the above scheme, in step S1, the yam, wolfberry, lotus seeds, hawthorn and hericium erinaceus are crushed and passed through a 30-80 mesh sieve, and dried at a temperature of 30-60° C. for 8-16 hours to obtain plant raw material powder.

[0024] As a further improvement of the above scheme, the solid-liquid ratio of the plant raw material powder to the extractant in step S2 is 1g:10-40mL, the ultrasonic power is 200-400W, the microwave power is 50-400W, and the extraction time is 5-10min; after the extraction, centrifugal filtration is performed to obtain the plant extract, the centrifugal speed is 8000r / min-12000r / min, the centrifugal time is 10min-20min, and a 0.45um organic phase filter membrane is selected for filtration.

[0025] As a further improvement of the above scheme, the plant re-extraction solution in step S3 can be a single re-extraction solution or a multiple re-extraction solution, wherein the preparation method of the multiple re-extraction solution is: using the previous plant re-extraction solution as an extractant, adding the plant raw material powder in step S1 multiple times to perform repeated extraction according to step S2 to obtain a plant re-extraction solution extracted multiple times.

[0026] As a further improvement of the above scheme, in step S4, the stirring time is 30-90 min, the temperature is 30-60° C., the stirring frequency is 300-1000 r / min, and the drying is performed using a spray dryer with a spray pressure of 5-30 kPa and an air volume of 0.2-1 m 3 / min, solution flow rate is 50-300mL / h, inlet temperature is 90-115℃, and outlet temperature is 80-95℃.

[0027] As a further improvement of the above scheme, in step S4, the stirring time is 45 min, the temperature is 50° C., the stirring frequency is 500 r / min, and the drying is performed using a spray dryer with a spray pressure of 15 kPa and an air volume of 0.6 m 3 / min, the solution flow rate is 150mL / h, the inlet temperature is 98℃, and the outlet temperature is 85℃.

[0028] As a further improvement of the above scheme, in step S5, the stirring frequency is 300-1000 r / min, and the stirring time is 10-60 min; preferably, the stirring frequency is 800 r / min, and the stirring time is 60 min.

[0029] The third aspect of the present invention provides an application of the composition as described in the first aspect or the composition obtained by the preparation method as described in the second aspect, including preparing an oral preparation for weight gain using the composition as a raw material.

[0030] As a further improvement of the above solution, the oral preparation is an oral powder.

[0031] Compared with the prior art, the present invention has the following beneficial effects:

[0032] (1) Safe ingredients and synergistic effects: Chinese yam, wolfberry, lotus seeds, hawthorn and hericium erinaceus are selected as raw materials that are both medicinal and edible. Chinese yam strengthens the spleen and stomach, wolfberry nourishes the liver and kidneys, lotus seeds nourish the spleen and stop diarrhea, hawthorn helps digestion and eliminates accumulation, and hericium erinaceus strengthens the spleen and nourishes the stomach. The various ingredients work together to help improve gastrointestinal function and promote nutrient absorption; protein powder and peptide powder can provide the body with a sufficient source of protein, which plays a key role in increasing muscle mass for thin people; compound probiotics help regulate the balance of intestinal flora, further improve the digestion and absorption capacity of the intestine, and create a more favorable intestinal environment for the absorption of nutrients; vitamins participate in the regulation of various physiological activities of the body, help maintain normal physiological functions of the body, and enhance immunity; the composition of the present invention improves the physical condition of thin people in all aspects through the synergistic effect of multiple safe ingredients, achieving the purpose of weight gain and improving physical fitness;

[0033] (2) High-efficiency and high-quality raw material extraction process: The ultrasonic-microwave synergistic extraction process is used to extract the active ingredients in the plant raw materials. The synergistic effect of the ultrasonic cavitation effect and the high-frequency electromagnetic field of the microwave greatly increases the contact area and mass transfer rate between the extractant and the plant raw materials, which can accelerate the dissolution of the target ingredients, with high extraction efficiency and low energy consumption;

[0034] (3) Obvious fattening effect: The fattening composite powder prepared with the composition of the present invention as raw material has good stability and antioxidant properties. Its excellent fattening effect has been proven by mouse experiments and can be used to solve the health problems of thin people. DETAILED DESCRIPTION

[0035] In order to make the technical problems, technical solutions and beneficial effects to be solved by the present invention more clearly understood, the present invention is further described in detail below in conjunction with the embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0036] The weight of the relevant components mentioned in the embodiments of the present invention description can not only refer to the specific content of each component, but also represent the proportional relationship of the weights of the components. Therefore, as long as the content of the relevant components in the embodiments of the present invention description is proportionally enlarged or reduced, it is within the scope disclosed in the embodiments of the present invention description. Specifically, the mass described in the embodiments of the present invention description can be a mass unit known in the chemical industry such as μg, mg, g, kg, etc.

[0037] In order to facilitate the understanding of the present invention, the present invention will be described more comprehensively and carefully in combination with the embodiments below, but the protection scope of the present invention is not limited to the following specific embodiments.

[0038] Unless otherwise defined, all professional terms used below have the same meanings as those generally understood by those skilled in the art. The professional terms used herein are only for the purpose of describing specific embodiments and are not intended to limit the scope of protection of the present invention.

[0039] Unless otherwise specified, various raw materials, reagents, instruments and equipment used in the present invention can be purchased from the market or prepared by existing methods.

[0040] Example 1

[0041] S1: According to weight, 40 parts of yam, 15 parts of wolfberry, 10 parts of lotus seeds, 10 parts of hawthorn, and 15 parts of Hericium erinaceus were washed with clean water, crushed, sieved through 60 mesh, and dried at 50°C for 12h to obtain plant raw material powder;

[0042] S2: Using ultrapure water as the extractant, the plant raw material powder obtained in step S1 was mixed with the extractant at a solid-liquid ratio of 1g:30mL, and then extracted in a microwave-ultrasonic combined extractor with an ultrasonic power of 300W, a microwave power of 100W, and an extraction time of 10min. After the extraction, the mixture was allowed to stand and cool to room temperature, centrifuged at a speed of 10000r / min for 12min, and filtered with a 0.45um organic phase filter membrane to obtain plant extract L1.

[0043] Example 2

[0044] The ultrasonic power in step S2 of Example 1 was adjusted to 200 W, and the parameters of the other steps were the same as those of Example 1 to obtain plant extract L2.

[0045] Example 3

[0046] The ultrasonic power in step S2 of Example 1 was adjusted to 400 W, and the parameters of the other steps were the same as those of Example 1 to obtain plant extract L3.

[0047] Example 4

[0048] The microwave power in step S2 of Example 1 was adjusted to 50 W, and the parameters of the other steps were the same as those of Example 1 to obtain plant extract L4.

[0049] Example 5

[0050] The microwave power in step S2 of Example 1 was adjusted to 200 W, and the parameters of the other steps were the same as those of Example 1 to obtain plant extract L5.

[0051] Example 6

[0052] The microwave power in step S2 of Example 1 was adjusted to 300 W, and the parameters of the other steps were the same as those of Example 1 to obtain plant extract L6.

[0053] Example 7

[0054] The extraction time in step S2 of Example 1 was adjusted to 2.5 min, and the parameters of other steps were the same as those of Example 1, to obtain plant extract L7.

[0055] Example 8

[0056] The extraction time in step S2 of Example 1 was adjusted to 5 min, and the parameters of the other steps were the same as those of Example 1, to obtain plant extract L8.

[0057] Example 9

[0058] The extraction time in step S2 of Example 1 was adjusted to 15 min, and the parameters of the other steps were the same as those of Example 1, to obtain plant extract L9.

[0059] Comparative Example 1

[0060] S1: According to weight proportions, 40 parts of yam, 15 parts of wolfberry, 10 parts of lotus seeds, 10 parts of hawthorn, and 15 parts of Hericium erinaceus were washed with clean water, crushed, sieved through 60 mesh, and dried at 50° C. for 12 h to obtain plant raw material powder;

[0061] S2: The plant raw material powder obtained in step S1 is mixed with ultrapure water at a solid-liquid ratio of 1 g:30 mL, and then extracted in a microwave-ultrasonic combined extraction instrument, with an ultrasonic power of 300 W, a microwave power of 0 W, and an extraction time of 10 min. After the extraction is completed, the mixture is allowed to stand and cool to room temperature to obtain a mixed solution;

[0062] S3: Add a complex enzyme formed by papain and cellulase in a ratio of 1:1 to the mixed solution obtained in step S2, wherein the complex enzyme is added at a rate of 0.03 times the mass of the plant raw material powder, the enzymolysis temperature is 50°C, the enzymolysis time is 1 hour, and the enzyme is inactivated after the enzymolysis is completed. The enzyme inactivation temperature is 85°C and the enzyme inactivation time is 60 minutes. After the enzyme inactivation is completed, centrifuge at a speed of 10,000 r / min for 12 minutes, and filter with a 0.45 um organic phase filter membrane to obtain plant extract D1.

[0063] Comparative Example 2

[0064] The ultrasonic power in step S2 of comparative example 1 was adjusted to 0 W, the microwave power was adjusted to 100 W, and the parameters of the remaining steps were the same as those of comparative example 1, to obtain plant extract D2.

[0065] Test Example 1: Determination of polysaccharide yield

[0066] The plant extracts prepared in the above Examples 1-9 and Comparative Examples 1-2 were tested for polysaccharide yields according to the following test methods.

[0067] Make a standard curve: First, prepare a series of standard glucose solutions of known concentrations. Accurately weigh 50 mg of standard glucose into a 500 mL volumetric flask and add water to the mark. Then aspirate different volumes of standard solution and make up to 2.0 mL with distilled water. Then add 1.0 mL of 6 vol% phenol aqueous solution and 5.0 mL of 98% concentrated sulfuric acid to each sample tube, shake well and let stand for 30 minutes. Measure the absorbance using a UV spectrophotometer at a wavelength of 490 nm. Use distilled water as a blank control using the same color development operation. Draw a standard curve based on the concentration of standard glucose and the corresponding absorbance.

[0068] Sample determination: Take 1.0 mL of each plant extract sample, dilute it 100 times with water, take 1 mL of each diluted sample, add 1.0 mL of distilled water, then add 1.0 mL of 6 vol% phenol aqueous solution, and quickly add 5.0 mL of 98% concentrated sulfuric acid. Oscillate on a vortex to mix thoroughly, let it stand for 30 minutes, and then measure the absorbance at a wavelength of 490 nm.

[0069] Calculation of results: Substitute the measured sample absorbance into the standard curve equation: y = mx + b, where: y is the sample absorbance (A value), x is the polysaccharide concentration, m is the slope of the linear regression, representing the relationship between polysaccharide concentration and absorbance, and b is the intercept. Calculate the concentration of polysaccharides in the diluted sample, and convert the actual concentration C after the extraction according to the dilution multiple. Calculate the polysaccharide yield according to the volume V of the extractant and the mass M of the plant raw material: Y = C*V / M. The test results are shown in Table 1.

[0070] Table 1 Extraction effect of different extraction methods on plant polysaccharides

[0071]

[0072]

[0073] The results show that changes in ultrasound and microwave power will significantly affect the extraction of polysaccharides from plants. Ultrasound can produce cavitation, increase the contact area between plant raw materials and extractants, and is beneficial to the extraction of active ingredients. If the ultrasound power is too large, the molecular vibration amplitude will increase, which is not conducive to the escape of active ingredients from the cavity structure, resulting in a decrease in the extraction rate; microwaves have a thermal effect, which is beneficial to molecular thermal motion and can accelerate the infiltration of active ingredients from the structure. If the microwave power is too large, the temperature will be too high, which may destroy the structure of polysaccharides and lead to a decrease in the extraction rate. By giving full play to the synergistic effect of ultrasound and microwaves, the contact area and mass transfer rate between the extractant and the plant raw materials can be greatly increased, the dissolution of the target ingredients can be accelerated, the extraction efficiency can be improved, and energy consumption can be reduced.

[0074] Example 10

[0075] S1: According to weight proportions, 40 parts of yam, 15 parts of wolfberry, 10 parts of lotus seeds, 10 parts of hawthorn, and 15 parts of Hericium erinaceus were washed with clean water, crushed, sieved through 60 mesh, and dried at 50° C. for 12 h to obtain plant raw material powder;

[0076] S2: Using ultrapure water as an extractant, the plant raw material powder obtained in step S1 is mixed with the extractant at a solid-liquid ratio of 1 g:30 mL, and then subjected to ultrasonic-microwave synergistic extraction in a microwave-ultrasonic combined extraction instrument. The ultrasonic power is 300 W, the microwave power is 100 W, and the extraction time is 10 min. After the extraction is completed, the mixture is allowed to stand and cool to room temperature, and then centrifuged and filtered to obtain a plant extract;

[0077] S3: using the plant extract obtained in step S2 as an extractant, mixing it with the plant raw material powder shown in the new step S1, extracting the new plant raw material according to the operation of step S2 to obtain a plant extract obtained after 1 extraction; using the plant extract obtained after 1 extraction as an extractant, mixing it with the plant raw material powder shown in the new step S1, and then extracting according to the process of step S2 to obtain a plant extract obtained after 2 extractions; using the plant extract obtained after 2 extractions as an extractant, mixing it with the plant raw material powder shown in the new step S1, and then extracting according to the process of step S2 to obtain a plant extract obtained after 3 extractions, named plant extract L10;

[0078] S4: According to the weight ratio, 78 parts of plant extract L10 were mixed with 5 parts of maltodextrin, 10 parts of microcrystalline cellulose, 3 parts of xylitol, 2 parts of citric acid and 2 parts of taurine, stirred at 50°C and 500 r / min for 45 min, and then dried by spray dryer with a spray pressure of 15 kPa and an air volume of 0.6 m 3 / min, the solution flow rate was 150mL / h, the inlet temperature was 98°C, and the outlet temperature was 85°C to form a spray-dried powder;

[0079] S5: Stir and blend 50 parts of the spray-dried powder with 10 parts of concentrated whey protein powder, 15 parts of isolated whey protein powder, 10 parts of bone collagen peptide powder, 5 parts of cod collagen peptide powder, 5 parts of Lactobacillus rhamnosus, 4 parts of Bifidobacterium animalis subsp. lactis, 0.2 parts of vitamin B2, 0.3 parts of vitamin B6, and 0.5 parts of vitamin D at a stirring frequency of 800 r / min and a stirring time of 60 min to obtain a composition, which is packaged and sealed to obtain a fattening composite powder L10.

[0080] Comparative Example 3

[0081] S1: According to weight proportions, 40 parts of yam, 15 parts of wolfberry, 10 parts of lotus seeds, 10 parts of hawthorn, and 15 parts of Hericium erinaceus were washed with clean water, crushed, sieved through 60 mesh, and dried at 50° C. for 12 h to obtain plant raw material powder;

[0082] S2: Using ultrapure water as an extractant, the plant raw material powder obtained in step S1 is mixed with the extractant at a solid-liquid ratio of 1 g:30 mL, and then extracted in a microwave-ultrasonic combined extraction instrument, with an ultrasonic power of 300 W, a microwave power of 0 W, and an extraction time of 10 min. After the extraction is completed, the mixture is allowed to stand and cool to room temperature, and then centrifuged to obtain a mixed solution;

[0083] S3: adding a composite enzyme formed by papain and cellulase in a ratio of 1:1 to the mixed solution obtained in step S2, wherein the composite enzyme is added in an amount of 0.03 times the mass of the plant raw material powder, the enzymolysis temperature is 50°C, the enzymolysis time is 1h, and after the enzymolysis is completed, the enzyme is inactivated at a temperature of 85°C and a time of 60min. After the enzyme inactivation is completed, the mixture is centrifuged at a speed of 10000r / min for 12min, and filtered with a 0.45um organic phase filter membrane to obtain a plant extract;

[0084] S4: using the plant extract obtained in step S3 as an extractant, extracting it with the plant raw material powder shown in the new step S1 according to the same process as steps S2 and S3 to obtain a plant extract solution extracted once; then using the plant extract solution extracted once as an extractant, extracting it with the plant raw material powder shown in the new step S1 according to the same process as steps S2 and S3 to obtain a plant extract solution extracted twice; using the plant extract solution extracted twice as an extractant, extracting it with the plant raw material powder shown in the new step S1 according to the same process as steps S2 and S3 to obtain a plant extract solution extracted three times, named plant extract solution D3;

[0085] S5: According to weight, 78 parts of plant extract D3 were mixed with 5 parts of maltodextrin, 10 parts of microcrystalline cellulose, 3 parts of xylitol, 2 parts of citric acid and 2 parts of taurine, stirred at 50°C and 500 r / min for 45 min, and then passed through a spray dryer with a spray pressure of 15 kPa and an air volume of 0.6 m 3 / min, the solution flow rate was 150mL / h, the inlet temperature was 98°C, and the outlet temperature was 85°C to form a spray-dried powder;

[0086] S6: By weight, 50 parts of the spray-dried powder obtained in step S5 are mixed with 10 parts of concentrated whey protein powder, 15 parts of isolated whey protein powder, 10 parts of bone collagen peptide powder, 5 parts of cod collagen peptide powder, 5 parts of Lactobacillus rhamnosus, 4 parts of Bifidobacterium animalis subsp. lactis, 0.2 parts of vitamin B2, 0.3 parts of vitamin B6 and 0.5 parts of vitamin D at a stirring frequency of 800 r / min for 60 min to obtain a composition, which is then packaged and sealed to obtain a fattening composite powder D3.

[0087] Comparative Example 4

[0088] S1: According to weight, 40 parts of yam, 15 parts of wolfberry, 10 parts of lotus seeds, 10 parts of hawthorn, and 15 parts of Hericium erinaceus were washed with water, crushed, sieved through 60 mesh, and dried at 50°C for 12h to obtain plant raw material powder;

[0089] S2: Using ultrapure water as an extractant, the plant raw material powder obtained in step S1 and the extractant were mixed at a solid-liquid ratio of 1 g:30 mL, and then subjected to microwave extraction in a microwave-ultrasonic combined extraction instrument, with an ultrasonic power of 0 W, a microwave power of 100 W, and an extraction time of 10 min. After the extraction was completed, the mixture was allowed to stand and cool to room temperature to obtain a mixed solution;

[0090] S3: adding compound enzyme (the ratio of papain to cellulase is 1:1) to the mixed solution obtained in step S2, the compound enzyme to plant raw material powder feed ratio is 0.03 g / g, the enzymatic hydrolysis temperature is set to 50°C, the enzymatic hydrolysis time is 1 hour, and after the enzymatic hydrolysis is completed, the enzyme is inactivated at a temperature of 85°C and a time of 60 minutes. After the enzyme inactivation is completed, centrifugal filtration is performed to obtain a plant extract;

[0091] S4: using the plant extract obtained in step S3 as an extractant, extracting it with the plant raw material powder shown in the new step S1 according to the same process as steps S2 and S3 to obtain a plant extract obtained after one extraction; using the plant extract obtained after one extraction as an extractant, extracting it with the plant raw material powder shown in the new step S1 according to the same process as steps S2 and S3 to obtain a plant extract obtained after two extractions; using the plant extract obtained after two extractions as an extractant, extracting it with the plant raw material powder shown in the new step S1 according to the same process as steps S2 and S3 to obtain a plant extract obtained after three extractions, named plant extract D4;

[0092] S5-S6: Replace the plant extract D3 in step S5 of comparative example 3 with the plant extract D4, and the remaining operations are the same as steps S5-S6 in comparative example 3 to obtain a composition, which is packaged and sealed to obtain a fattening composite powder D4.

[0093] Test Example 2: Effect of repeated extraction times on polysaccharides in plant extracts

[0094] The polysaccharide contents of the plant extracts obtained by re-extraction 1, 2 and 3 times in Example 10, Comparative Example 3 and Comparative Example 4 were measured respectively. The test results are shown in Table 2.

[0095] The results showed that with the increase of the number of re-extractions, the polysaccharide concentrations in the plant re-extraction solutions obtained in Example 10, Comparative Example 3, and Comparative Example 4 with different extraction times gradually increased, but the relative increments of the polysaccharide content in the plant re-extraction solutions obtained in Comparative Example 3 and Comparative Example 4 gradually decreased with the increase of the number of re-extractions; while the relative increments of the polysaccharide content in the plant extract obtained in Example 10 remained at a high level with the increase of the number of extractions, indicating that the ultrasonic-microwave synergistic extraction has high extraction efficiency and good repeatability for the extraction of active substances such as polysaccharides in plant raw materials.

[0096] Table 2 Effect of repeated extraction times on polysaccharides in plant extracts

[0097]

[0098] Test Example 3: Effect of natural light storage time at room temperature on the polysaccharide content in the fattening compound powder

[0099] The fattening composite powders prepared in Example 10 and Comparative Examples 3 and 4 were placed at room temperature under natural light. After a certain period of time, the polysaccharide content in the samples was detected. The results are shown in Table 3.

[0100] The results showed that as the storage time increased, the polysaccharide content in the three groups of fattening compound powders decreased to a certain extent, but until 8 months of storage, the polysaccharide content in each group of fattening compound powders could still be maintained at more than 95%, which shows that making the composition of the present invention into an oral powder can improve the stability of the product, reduce the decomposition of active substances such as polysaccharides, and facilitate long-term storage.

[0101] Table 3 Effect of different room temperature storage time on polysaccharide content in fattening compound powder

[0102]

[0103] Test Example 4: DPPH free radical scavenging test

[0104] The fattening composite powders L10, D3 and D4 prepared in Example 10, Comparative Example 3 and Comparative Example 4 were respectively subjected to DPPH free radical scavenging test according to the following method:

[0105] Using water as the solvent, prepare fertilizer compound powder solutions of different concentrations (0.0625 mg / mL, 0.125 mg / mL, 0.25 mg / mL, 0.5 mg / mL, 1 mg / mL), set up sample tubes (T), sample background (T0), DPPH tubes (C) and solvent background (C0), and 3 parallel tubes are required for the sample tube (T) of each fertilizer compound powder solution.

[0106] Exemplarily, taking a 1 mg / mL fattening composite powder solution as an example, the following sample tube (T), sample background (T0), DPPH tube (C) and solvent background (C0) are set:

[0107] Sample tube (T): 1 mL of fertilizer compound powder solution (concentration of 1 mg / mL) + 2 mL of water + 1 mL of DPPH ethanol solution (concentration of 0.04 mg / mL);

[0108] Sample background tube (T0): 1 mL of fertilizer compound powder solution (concentration 1 mg / mL) + 2 mL of water + 1 mL of 95 vol% ethanol solution;

[0109] DPPH tube (C): 3 mL of water + 1 mL of DPPH ethanol solution (concentration 0.04 mg / mL);

[0110] Solvent background tube (C0): 3 mL water + 1 mL 95% vol ethanol solution;

[0111] The solutions in the above reaction tubes were transferred into cuvettes, the absorbance was measured at 517 nm, and the DPPH radical scavenging rates of different fattening composite powder solutions were calculated by formula I. The specific results are shown in Table 4.

[0112] Formula I:

[0113] The results show that the fattening composite powder prepared in the present invention has a good scavenging effect on DPPH free radicals, and the fattening composite powder L10 prepared in Example 10 has better scavenging ability on DPPH free radicals than the fattening composite powders D3 and D4 prepared in Comparative Examples 3 and 4.

[0114] Table 4 DPPH radical scavenging rate of different fertilizer composite powder solutions

[0115] concentration 0.0625mg / mL 0.125mg / mL 0.25mg / mL 0.5mg / mL 1mg / mL Fertilizer compound powder L10 solution 24.63％ 37.42％ 54.75％ 75.27％ 93.45％ Fertilizer compound powder D3 solution 13.57％ 20.49％ 40.34％ 59.54％ 82.37％ Fertilizer compound powder D4 solution 18.91％ 36.57％ 47.88％ 68.57％ 89.30％

[0116] Test Example 5: Effects of fattening compound powder on growth performance and immune function of mice

[0117] 1. Experimental Methods

[0118] (1) Mouse culture: 40 male mice with a body weight of 18-22 g were selected and fed with adaptive basic feed for one week before the experiment. Ten mice were randomly selected as the normal diet group and fed with basic feed. The remaining 30 mice were randomly divided into three groups of 10 mice each as L10 group, D3 group and D4 group, and fed with 1 g / kg of fattening compound powder L10, fattening compound powder D3 and fattening compound powder D4 added to the basic feed respectively. After 7 days of adaptive feeding, the formal experiment began, and the formal experiment lasted for 28 days. The breeding environment was 2-3 rats per cage, the breeding temperature was 22±2℃, the humidity was 60-70%, and the fluorescent lighting and dark environment were alternated every 12 hours. The basic feed formula was 55% nitrogen-free extract, 18% crude protein, 10% moisture, 8% crude ash, 5% crude fiber, and 4% crude fat; the calcium addition was 1.5% and the phosphorus addition was 1%. The animals and materials required for the experiment were all from the College of Animal Science and Technology of Hunan Agricultural University.

[0119] (2) Determination of growth performance indexes: The mice were weighed at 08:00 on the first day of the experiment as the initial body weight, and at 08:00 on the day the experiment ended as the final body weight. The remaining feed amount and the newly added feed amount of the previous day were weighed at 08:00 every day. The average daily weight gain (ADG), average daily feed intake (ADFI) and feed-to-weight ratio (F / G) of each group of experimental mice were calculated:

[0120] ADG (g) = total weight gain (g) / test days (d);

[0121] ADFI (g) = total feed intake (g) / experimental days (d);

[0122] F / G = total feed intake (g) / total weight gain (g).

[0123] (3) Serum immunoglobulin detection: After the test, blood was collected through the eye socket and centrifuged at 3000 r / min for 10 min to collect serum. The levels of immunoglobulin A (IgA), immunoglobulin M (IgM), and immunoglobulin G (IgG) in the serum were determined using ELISA enzyme-linked immunosorbent assay kit.

[0124] (3) Determination of organ index: Mice were killed by cervical dislocation, spleen and thymus were collected, surrounding connective tissue and fat were stripped and dried with filter paper, organs were weighed and recorded, and the organ index was calculated. Organ index (mg / g) = organ fresh weight (mg) / live weight (g).

[0125] 2. Experimental results

[0126] (1) Effects of fattening compound powder on growth performance of mice

[0127] The results of the growth performance indexes of mice in each group are shown in Table 5. The results showed that compared with the mice in the normal diet group, the average daily weight gain of mice in the L10, D3, and D4 groups increased, among which the increase in the L10 group was the largest. At the same time, the average daily feed intake and feed-to-weight ratio of mice in the L10, D3, and D4 groups were also lower than those in the normal diet group, among which the L10 group had the lowest.

[0128] Table 5 Effect of fattening compound powder on growth performance of mice

[0129]

[0130]

[0131] (2) Effects of fattening compound powder on serum immune indexes of mice

[0132] The results of serum immunoglobulin test of each group of mice are shown in Table 6. The results showed that the IgA and IgG levels of mice in the L10, D3 and D4 groups were higher than those in the normal diet group, among which the IgA and IgG levels of mice in the L10 group were the highest; there was no significant difference in IgM between the three groups of mice and the normal diet group.

[0133] Table 6 Effects of fattening compound powder on serum immune indexes of mice

[0134] Group IgA (μg / mL) IgG (μg / mL) IgM (μg / mL) Normal diet group 153.45 16.88 1.48 L10 Group 190.38 21.35 1.49 D3 Group 177.21 19.21 1.49 D4 Group 185.65 20.04 1.48

[0135] (3) Effects of fattening compound powder on organ indexes of mice

[0136] The results of organ index determination of mice in each group are shown in Table 7. The results showed that compared with the mice in the normal diet group, the thymus index and spleen index of mice in the L10, D3, and D4 groups were all increased, among which the organ index of mice in the L10 group was the most significantly increased.

[0137] Table 7 Effects of fattening compound powder on organ indexes of mice

[0138] Group Thymus index (mg / g) Spleen index (mg / g) Normal diet group 2.73 5.15 L10 Group 2.95 5.73 D3 Group 2.78 5.32 D4 Group 2.88 5.55

[0139] The above description is only a preferred embodiment of the present application and is not intended to limit the present application. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present application should be included in the protection scope of the present application.

Claims

1. A composition having a fattening effect, characterized in that: The invention comprises yam, wolfberry, lotus seed, hawthorn, hericium erinaceus, protein powder, peptide powder, compound probiotic powder and vitamins, wherein the protein powder is at least one of concentrated whey protein powder, isolated whey protein powder, casein powder and soy protein powder; the peptide powder is at least one of bovine spleen peptide powder, yak bone oligopeptide powder, bone collagen peptide powder, corn peptide powder and cod collagen peptide powder; the compound probiotic powder is at least one of Lactobacillus plantarum, Lactococcus lactis lactis subsp. lactis, Streptococcus thermophilus, Lactobacillus rhamnosus and Bifidobacterium animalis subsp. lactis; and the vitamins are at least two of vitamin A, vitamin B1, vitamin B2, vitamin B6, vitamin D, vitamin E and vitamin K.

2. The composition according to claim 1, characterized in that The composition further comprises excipients, sweeteners and flavoring agents, wherein the excipient is at least one of lactose, maltodextrin, cyclodextrin and microcrystalline cellulose, the sweetener is at least one of xylitol, mannitol, maltitol, isomalt oligosaccharide, sorbitol and sucrose, and the flavoring agent is at least one of citric acid, malic acid, tartaric acid and taurine.

3. A method for preparing the composition according to any one of claims 1 to 2, characterized in that: The following steps are involved: S1: crushing and drying yam, wolfberry, lotus seeds, hawthorn, and hericium erinaceus to obtain plant raw material powder; S2: using water as an extractant, mixing the plant raw material powder with the extractant and performing ultrasonic-microwave synergistic extraction to obtain a plant extract; S3: using the plant extract as an extractant, adding the plant raw material powder in step S1 to perform repeated extraction according to step S2 to obtain a plant multiple extraction solution; S4: mixing the plant extract with excipients, sweeteners and flavoring agents, stirring and drying to obtain a spray-dried powder; S5: stirring and blending the spray-dried powder with protein powder, peptide powder, composite probiotic powder and vitamins to obtain a composition.

4. The preparation method according to claim 3, characterized in that: In step S1, by weight, yam is 20-50 parts, wolfberry is 10-30 parts, lotus seeds is 10-30 parts, hawthorn is 5-15 parts, and Hericium erinaceus is 10-30 parts.

5. The preparation method according to claim 3, characterized in that: In step S4, the plant extract is 70-90 parts by weight, the excipient is 5-20 parts by weight, the sweetener is 1-5 parts by weight, and the flavoring agent is 1-5 parts by weight; The excipient consists of maltodextrin and microcrystalline cellulose, the sweetener consists of xylitol, and the flavoring agent consists of citric acid and taurine.

6. The preparation method according to claim 3, characterized in that: In the step S5, the spray-dried powder is 30-60 parts, the protein powder is 10-30 parts, the peptide powder is 10-20 parts, the composite probiotic powder is 5-10 parts, and the vitamins are 0.1-3 parts in parts by weight; the protein powder is composed of concentrated whey protein powder and isolated whey protein powder, the peptide powder is composed of bone collagen peptide powder and cod collagen peptide powder, the composite probiotic powder is composed of rhamnosus Lactobacillus and animal Bifidobacterium lactis subsp. lactis, and the vitamins are composed of vitamin B2, vitamin B6 and vitamin D.

7. The preparation method according to any one of claims 3 to 6, characterized in that: The following steps are involved: S1: According to weight, 40 parts of yam, 15 parts of wolfberry, 10 parts of lotus seeds, 10 parts of hawthorn and 15 parts of Hericium erinaceus were crushed and dried to obtain plant raw material powder; S2: Using ultrapure water as an extractant, the plant raw material powder and the extractant are mixed at a solid-liquid ratio of 1 g:5-80 mL, and then subjected to ultrasonic-microwave synergistic extraction, with an ultrasonic power of 100-600 W, a microwave power of 50-800 W, and an extraction time of 1-60 min to obtain a plant extract; S3: using the plant extract obtained in step S2 as an extractant, adding the plant raw material powder in step S1 to perform repeated extraction according to step S2 to obtain a plant multiple extraction solution; S4: Mix 78 parts of the plant extract with 5 parts of maltodextrin, 10 parts of microcrystalline cellulose, 3 parts of xylitol, 2 parts of citric acid and 2 parts of taurine by weight, stir and dry to obtain a spray-dried powder; S5: By weight, 50 parts of spray-dried powder, 10 parts of concentrated whey protein powder, 15 parts of isolated whey protein powder, 10 parts of bone collagen peptide powder, 5 parts of cod collagen peptide powder, 5 parts of Lactobacillus rhamnosus, 4 parts of Bifidobacterium animalis subsp. lactis, 0.2 parts of vitamin B2, 0.3 parts of vitamin B6 and 0.5 parts of vitamin D are stirred and blended to obtain a composition.

8. Use of the composition according to any one of claims 1 to 2 or the composition obtained by the preparation method according to any one of claims 3 to 7, characterized in that: The invention comprises using the composition as a raw material to prepare an oral preparation for weight gain.

9. The use according to claim 8, characterized in that The oral preparation is an oral powder.

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