Composition for preventing hair loss and strengthening hair as well as preparation method and application of composition

Abstract

The invention discloses an anti-hair-loss and hair-strengthening composition as well as a preparation method and application thereof, and particularly belongs to the field of preparations for dressing and makeup. The hair loss preventing and hair strengthening composition comprises a compound fermentation product filtrate and a silk glue protein concentrated solution, the preparation method of the composite fermentation product filtrate comprises the following steps: mixing mulberry leaves, safflower, cacumen biotae, agilawood and selfheal, crushing, and sieving to obtain composite powder; extracting the composite powder by adopting an ethanol solution to obtain a crude extracting solution; carrying out domestication culture on the leuconostoc citrinum by adopting the crude extracting solution to obtain a domesticated compound bacterium solution; and carrying out fermentation culture on the domesticated compound bacteria liquid, centrifuging, taking supernate, filtering and sterilizing to obtain a compound fermentation product filtrate. The hair loss preventing and fixing composition provided by the invention has good hair loss preventing and fixing effects, and the hair follicle density improvement rate reaches 70% or above.

Description

Technical Field

[0001] The invention belongs to the field of cosmetic preparations, and in particular relates to a hair loss prevention and hair solidification composition and a preparation method and application thereof. Background Art

[0002] Hair loss, as a common physiological phenomenon, has a complex and diverse mechanism of occurrence. Generally speaking, it can be divided into two categories: physiological hair loss and non-physiological hair loss. Hair growth follows a natural cyclical process, covering the growth phase, regression phase and resting phase. In a healthy state, after the hair has fully grown during the growth phase, it naturally enters the regression and resting phases, resulting in a certain amount of physiological shedding. During this process, the replacement of old and new hair maintains a dynamic balance and ensures the relative stability of hair volume. Usually, the number of hairs that an individual loses naturally every day is between 50 and 100, which is considered a normal physiological phenomenon.

[0003] However, when the amount of hair loss exceeds this range, especially when the average daily hair loss exceeds 100 hairs, the balance between hair growth and loss is broken, causing the hair to gradually become sparse, and even leading to baldness. This abnormal, excessive hair loss not only seriously affects personal appearance, but may also bring heavy psychological burdens and mental stress to patients. This type of hair loss caused by a variety of non-physiological factors, such as thinning hair and receding hairline, which significantly affects appearance, is classified as non-physiological hair loss, also known as pathological hair loss.

[0004] Pathological hair loss can be divided into five categories according to its main causes: androgenic alopecia, immunodeficiency alopecia, injury-induced alopecia, nutritional alopecia and drug-induced alopecia. As the problem of pathological hair loss becomes increasingly serious, many anti-hair loss products have emerged on the market to solve this problem. These products are mainly divided into two camps: one is based on the theory of traditional Chinese medicine, using a variety of herbal compound extracts to try to slow down hair loss through nourishing or oil control mechanisms, but consumer feedback shows that most products have limited effects; the other is the integration of Western medicine ingredients, such as sildenafil, minoxidil, etc. as active substances. Although they can significantly inhibit hair loss and promote hair growth in the early stage, the side effects associated with long-term use (such as sexual dysfunction, abnormally low blood pressure, etc.) cannot be ignored, which limits the feasibility of its long-term application.

[0005] Therefore, in view of the shortcomings of existing anti-hair loss products in terms of effectiveness and safety, there is an urgent need to develop a new anti-hair loss technology or product that can not only effectively prevent hair loss but also have good safety, so as to meet market demand and improve the quality of life of hair loss patients. Summary of the invention

[0006] In view of the defects of the prior art anti-hair loss and hair solidifying products that are not effective or have significant side effects, the purpose of the present invention is to provide an anti-hair loss and hair solidifying composition, which promotes hair follicle cell proliferation and hair growth by regulating the growth metabolism of hair follicle cells, inhibiting the release of androgens and inflammatory factors, and regulating the scalp microecology, thereby exerting the effect of anti-hair loss and hair solidifying, and is safe to the human body and has no side effects.

[0007] To achieve the above object, the present invention discloses the following technical solutions:

[0008] In a first aspect, the present invention provides a composition for preventing hair loss and fixing hair, comprising the following components in parts by mass:

[0009] 15-18 parts of composite fermentation product filtrate;

[0010] 1 part of silk fibroin concentrate;

[0011] The method for preparing the composite fermentation product filtrate comprises the following steps:

[0012] (1) Mix mulberry leaves, safflower, arborvitae leaves, agarwood, and selfheal, crush them, and sieve them to obtain a composite powder;

[0013] (2) extracting the composite powder with an ethanol aqueous solution, removing the ethanol, and obtaining a crude extract;

[0014] (3) using the crude extract to perform acclimatization and culture of Leuconostoc citrifolia to obtain an acclimatized composite bacterial solution;

[0015] (4) inoculating the domesticated composite bacterial liquid into a fermentation medium containing the crude extract for fermentation culture, then centrifuging to obtain the supernatant, filtering and sterilizing to obtain a composite fermentation product filtrate.

[0016] Preferably, in step (1), the mulberry leaves, safflower, Platycladus orientalis leaves, agarwood and selfheal are mixed in a mass ratio of (4.5-5.5):(0.09-0.11):(6-8):(1.8-2.2):(1.8-2.2).

[0017] Preferably, in step (2), the ethanol aqueous solution is a 50% v / v ethanol aqueous solution.

[0018] Preferably, the step (2) is to extract the composite powder with a 50% v / v ethanol aqueous solution at room temperature for 15 days, remove the ethanol after filtering, and obtain a crude extract.

[0019] Preferably, the step (3) is to inoculate the lemon-colored Leuconostoc into the acclimation culture medium containing the crude extract, and then culture it in a shaking incubator at a speed of 150-200 r / min and a temperature of 25-30° C. for 1-2 days to obtain an acclimated composite bacterial solution;

[0020] The step (4) is to dilute the acclimated composite bacterial solution with sterile water to a viable bacterial count of 1×10 7 -1×10 9 CFU / mL of diluted bacterial solution, and then inoculated the diluted bacterial solution into the fermentation medium at an inoculation rate of 9-12% v / v, fermented and cultured for 48-60 hours at a temperature of 25-28°C and a stirring speed of 150-200 r / min, and then centrifuged to obtain the supernatant, filtered and sterilized to obtain a composite fermentation product filtrate.

[0021] Further preferably, the acclimation medium comprises the following components: 10 wt% crude extract, carbon source, nitrogen source, vitamins, inorganic salts, deionized water, and a pH of 6.5±0.2;

[0022] The fermentation medium comprises the following components: 10 wt% of crude extract, a carbon source, a nitrogen source, vitamins, inorganic salts, deionized water, and a pH value of 6.5±0.2.

[0023] In a second aspect, the present invention provides the use of the anti-hair loss and hair solidifying composition described in the first aspect in the preparation of a hair care product having the efficacy of anti-hair loss and hair solidifying.

[0024] In a third aspect, the present invention provides an anti-hair loss and hair strengthening essence, comprising the anti-hair loss and hair strengthening composition of the first aspect;

[0025] The amount of the anti-hair loss and hair solidifying composition added to the anti-hair loss and hair solidifying essence is 10-50wt%.

[0026] Preferably, the invention further comprises a fibroin concentrate, a moisturizer, a preservative, a thickener, an emulsifier, a pH regulator and a solvent.

[0027] In a fourth aspect, the present invention provides a method for preparing the anti-hair loss and hair strengthening essence according to the third aspect, comprising the following steps:

[0028] The silk fibroin concentrate, the anti-hair loss and hair strengthening composition, moisturizer, thickener, emulsifier, preservative and solvent are mixed and stirred evenly, and then a pH regulator is added to adjust the pH to 6.5-7 to obtain an anti-hair loss and hair strengthening essence.

[0029] In the present invention:

[0030] Mulberry leaves can activate luciferase, prevent the occurrence of atopic dermatitis, have strong antioxidant properties, accelerate the biosynthesis of hyaluronic acid in the cortex, reduce the irritation of fruit acid, inhibit and regulate the activity of 5a-reductase, and promote hair growth; Prunella vulgaris can stimulate hair growth, activate enzyme tyrosinase, and have anti-inflammatory, antibacterial, and analgesic effects. It also has an inhibitory effect on 5a-reductase, prevents and treats hair loss caused by high androgen levels, activates tyrosinase activity, increases the production of melanin, inhibits methioninase, reduces the production of methyl mercaptan and reduces body odor; Agarwood has the function of inhibiting hyaluronic acid. The decomposition of hyaluronic acid enhances the skin's moisturizing effect and promotes hair growth. The resin components in agarwood also have the function of deep cleansing and absorbing dirt and oil, which can keep the skin fresh and clean; Platycladus orientalis leaves have the effects of cooling blood and stopping bleeding, resolving phlegm and relieving cough, and can also promote hair growth and black hair. It has a certain auxiliary therapeutic effect on blood-heat hair loss and premature graying of hair, and has the function of controlling oil and cleaning the scalp. Platycladus orientalis leaves can stimulate scalp blood circulation and play an antibacterial and anti-inflammatory role; safflower can be used to improve microcirculation, promote scalp blood circulation, thereby helping hair growth and reducing hair loss.

[0031] The present invention has found that lemon-colored Leuconostoc can enrich the effective ingredients in the crude extracts of mulberry leaves, safflower, arborvitae leaves, agarwood, and selfheal. At the same time, the fermentation of Leuconostoc itself can produce bacteriocins, organic acids, extracellular polysaccharides and other active substances, which have the effects of regulating scalp barrier function damage, hormone metabolism disorders, activating the physiological functions of hair follicles and hair bulbs, and promoting scalp blood circulation. In addition, it is also compounded with silk colloid protein, which has the effects of strong moisturizing and precise oil control, can effectively regulate the oil-water balance of the scalp, improve the scalp barrier function, and provide a good material basis for the healthy growth of hair.

[0032] Beneficial effects of the present invention:

[0033] 1. The anti-hair loss and hair-fixing composition provided by the present invention has the effect of promoting the proliferation of hair papilla cells and thus promoting hair growth;

[0034] 2. After the anti-hair loss and hair-fixing composition provided by the present invention acts on the scalp, it can increase the production of cytokines related to hair growth, resist the production of inhibitory factors, and has the effect of hair growth and hair fixing;

[0035] 3. The anti-hair loss and hair-fixing composition provided by the present invention containing the above-mentioned composition has a good effect of hair-fixing and hair-loss prevention, and the hair follicle density improvement rate reaches more than 70%. DETAILED DESCRIPTION

[0036] In order to make the purpose, technical scheme and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with specific embodiments. However, the following embodiments are only preferred embodiments of the present invention, not all. For the sake of clarity, all features of the actual embodiments are not described.

[0037] Based on the embodiments in the implementation manner, other embodiments obtained by those skilled in the art without making any creative work shall all fall within the protection scope of the present invention.

[0038] In the present invention

[0039] Mulberry leaves: Morus alba L. of the Moraceae family were purchased from Guangzhou Qingping Chinese Herbal Medicine Market;

[0040] Safflower: Carthamus tinctorius L., Asteraceae, purchased from Guangzhou Qingping Chinese Herbal Medicine Market;

[0041] Platycladus orientalis (L.) Franco, Cupressaceae, purchased from Guangzhou Qingping Chinese Medicine Market;

[0042] Agarwood: Agarwood from Aquilaria sinensis (Lour.) Spreng. of the Thymelaeaceae family, purchased from Guangzhou Qingping Chinese Herbal Medicine Market;

[0043] Prunella vulgaris: Prunella vulgaris L., Lamiaceae, purchased from Guangzhou Qingping Chinese Herbal Medicine Market;

[0044] Leuconostoc citreum: scientific name Leuconostoc citreum, strain number GDMCC NO.1.919, purchased from Guangdong Microbiological Culture Collection Center;

[0045] Sericin concentrate: a sericin concentrate prepared according to the preparation method of Example 1 in Chinese patent CN116083510B;

[0046] Yeast extract: purchased from Beijing Hongrun Baoshun Technology Co., Ltd.;

[0047] Silk fibroin concentrate: purchased from Suzhou Suhao Biomaterial Technology Co., Ltd.;

[0048] Other raw materials are commercially available.

[0049] Preparation of anti-hair loss and hair solidifying composition

[0050] Step (1), mixing dried mulberry leaves, safflower, arborvitae leaves, agarwood, and selfheal in a mass ratio of (4.5-5.5):(0.09-0.11):(6-8):(1.8-2.2):(1.8-2.2), and grinding through a 60-mesh sieve to obtain a composite powder;

[0051] Step (2), extracting the composite powder with a 50% v / v ethanol aqueous solution at room temperature for 15 days, removing the ethanol after filtering, and obtaining a crude extract;

[0052] Step (3), preparation of acclimation medium: crude extract 10wt%, glucose 2.5wt%, peptone 0.1wt%, beef extract 0.2wt%, KH2PO4 0.1wt%, MgSO4 0.15wt%, vitamin B1 0.01wt%, balance deionized water, pH = 6.5 ± 0.2;

[0053] Step (4), acclimatization and culture: scrape 0.5 cm 2 The activated slant strain of Leuconostoc citraceus is inoculated into the prepared acclimation medium, and then cultured in a shaker at a speed of 150-200 r / min and a temperature of 25-30° C. for 1-2 days to obtain an acclimated composite bacterial solution;

[0054] Step (5), preparation of fermentation medium: crude extract 10wt%, glucose 1.5wt%, peptone 0.1wt%, yeast extract 0.5wt%, KH2PO4 0.1wt%, MgSO4 0.15wt%, vitamin B1 0.01wt%, balance deionized water, pH = 6.5 ± 0.2;

[0055] Step (6), dilute the acclimated composite bacterial solution with sterile water to a viable bacterial count of 1×10 7 -1×10 9 CFU / mL of diluted bacterial solution, and then inoculating the diluted bacterial solution into the fermentation medium at an inoculation rate of 9-12% v / v, fermenting and culturing for 48-60 hours at a temperature of 25-28°C and a stirring speed of 150-200 r / min, then centrifuging to obtain the supernatant, filtering and sterilizing through a 0.22 μm polyethersulfone filter element to obtain a composite fermentation product filtrate;

[0056] Step (7), taking the composite fermentation product filtrate obtained in step (6) and the sericin protein concentrate in a mass ratio of (15-18):1 and mixing them to obtain an anti-hair loss and hair solidification composition.

[0057] Table 1 Preparation parameters of examples and comparative examples

[0058]

[0059] Table 1 Preparation parameters of examples and comparative examples (continued)

[0060]

[0061] Note: “-” in the table means not added.

[0062] Comparative Example 1:

[0063] The raw materials lack mulberry leaves, and the remaining steps are consistent with Example 2.

[0064] Comparative Example 2:

[0065] The raw materials lack safflower, and the remaining steps are consistent with Example 2.

[0066] Comparative Example 3:

[0067] The raw materials lack Platycladus orientalis leaves, and the remaining steps are consistent with Example 2.

[0068] Comparative Example 4:

[0069] There is no agarwood in the raw materials, and the remaining steps are consistent with Example 2.

[0070] Comparative Example 5:

[0071] The raw materials lack Prunella vulgaris, and the remaining steps are consistent with Example 2.

[0072] Comparative Example 6:

[0073] Without performing steps (3) to (6), the crude extract and the sericin concentrate are directly mixed to obtain an anti-hair loss and hair solidifying composition. The remaining steps are the same as those in Example 2.

[0074] Comparative Example 7:

[0075] The mass ratio of mulberry leaves, safflower, arborvitae leaves, agarwood, and selfheal was adjusted to 1:1:1:1:1, and the remaining steps were consistent with Example 2.

[0076] Performance Testing

[0077] Proliferation promotion experiment of dermal papilla cells

[0078] Cell culture: HFDPCs were cultured in Dulbecco's modified Eagle's medium (DMEM) containing 10% (v / v) fetal bovine serum and 1% (v / v) antibiotics at 37°C and 5% CO2.

[0079] Sample preparation: Examples 1-3 and Comparative Examples 1-7 were diluted with DMEM culture medium to a concentration of 10 wt % of the test sample.

[0080] CCK-8 assay: Dermal papilla cells were cultured at 5×10 4 / well were inoculated in a 96-well plate and incubated for 24 hours. 10 wt% of the sample to be tested was added respectively. Serum-free DMEM medium and serum-containing DMEM medium were used as blank control and negative control respectively. Three parallel treatments were performed in each group, and the results were averaged. After 24 hours of treatment, 10% CCK-8 solution was added to each well and incubated for another 1 hour. The absorbance OD was measured at a detection wavelength of 450 nm.

[0081] Results were calculated as follows: Cell viability (%) = (OD sample - OD blank) / (OD negative control - OD blank) × 100%. The results are shown in Table 2.

[0082] Table 2 Relative cell viability (%)

[0083]

[0084]

[0085] Result analysis:

[0086] Under co-incubation with the anti-hair loss and hair fixing composition, the cell viability was significantly improved compared with the negative control group without the anti-hair loss and hair fixing composition, up to 156.22%, proving that the anti-hair loss and hair fixing composition can promote the proliferation of hair papilla cells, thereby promoting hair growth.

[0087] Experimental study on regulation of cytokines related to hair growth in dermal papilla cells

[0088] Cell culture: HFDPCs were cultured in Dulbecco's modified Eagle's medium (DMEM) containing 10% (v / v) fetal bovine serum and 1% (v / v) antibiotics at 37°C and 5% CO2.

[0089] Sample preparation: Examples 1-3 and Comparative Examples 1-7 were diluted with DMEM medium to a concentration of 10 wt% of the test sample, minoxidil was diluted with DMEM medium to a concentration of 0.0002 wt% as a positive control group, and DMEM medium without any ingredients was added as a blank control group. Three parallel treatments were performed in each group, and the results were averaged.

[0090] Experimental method: The regulatory effects of the growth factors VEGF and TGF-β1 related to hair growth in the culture supernatant obtained after the sample was treated were measured by ELISA kit. The operation was carried out according to the instructions of the kit. The results are shown in Table 3.

[0091] Table 3 VEGF and TGF-β1 content

[0092]

[0093]

[0094] Result analysis:

[0095] After co-incubation with the anti-hair loss and hair-fixing composition, the amount of VEGF produced by human hair papilla cells increased. VEGF plays a role in the growth of hair follicles and is a landmark research indicator for promoting hair growth. On the other hand, minoxidil, as a positive control, significantly increased the amount of VEGF produced by human hair papilla cells compared with the blank control; in addition, both the positive control and the anti-hair loss and hair-fixing composition can significantly reduce the production of TGF-β1, which is produced by androgen after conversion by reductase and binding to androgen receptors, regulating the apoptosis process of hair papilla cells, leading to hair follicle atrophy and finally hair loss. Therefore, the results show that after the anti-hair loss and hair-fixing composition acts on the scalp, it can increase the production of hair growth-related cytokines and resist the production of inhibitory factors, thereby achieving the effect of hair growth and hair fixation.

[0096] Application Example Preparation

[0097] Deionized water, hydroxyethyl acrylate / methoxyethyl acrylate copolymer, 1,3-propylene glycol, butylene glycol, isoprene glycol and ethylhexylglycerol are added into a reactor and mixed and stirred evenly. Then, carbohydrate isomers, fibroin concentrate, capryloyl glycine, arginine, anti-hair loss and hair solidifying composition or composite fermentation product filtrate or sericin concentrate are sequentially added into the reactor and mixed and stirred evenly. Lactic acid is added to adjust the pH to 6.8 to prepare an anti-hair loss and hair solidifying essence.

[0098] Table 4 Raw materials and their mass percentages in the anti-hair loss and hair strengthening essence

[0099]

[0100] Note: “-” in the table means not added.

[0101] Evaluation of anti-hair loss effect

[0102] Experimental protocol:

[0103] 50 volunteers with hair loss, aged 30 to 55 years old, were selected. The subjects were randomly divided into 5 groups, 10 in each group, and applied Application Examples 1-5 respectively, once in the morning and once in the evening, taking 2 mL of sample solution each time and applying it to the scalp where hair loss occurs, and massage for 5 to 10 minutes, and used continuously for 6 months. During the trial, no other cosmetics, drugs or health products that may affect the results were used.

[0104] The hair follicle density of the subjects at the hair loss site (area 4cm×4cm) was detected by a hair follicle analyzer before and after use, and the average value was taken. The results are shown in Table 5.

[0105] At the same time, the subjects evaluated the improvement of hair loss symptoms before and after use. The results are shown in Table 6.

[0106] Table 5 Evaluation results of hair follicle density changes (number / cm 2 )

[0107] Group Before use After use Improvement rate / % Application Example 1 45.7 78.2 71.12％ Application Example 2 44.9 71.1 58.35％ Application Example 3 47.3 69.6 47.15％ Application Example 4 46.1 57.3 24.30％ Application Example 5 44.7 53.7 20.13％

[0108] Table 6 Evaluation results of hair loss changes (based on the number of evaluations)

[0109] Group No change Slight improvement Significant improvement Stop hair loss Application Example 1 0 0 7 3 Application Example 2 0 4 5 1 Application Example 3 0 8 2 0 Application Example 4 3 6 1 0 Application Example 5 6 4 0 0

[0110] Result analysis:

[0111] According to the effect verification of application examples 1-3, it can be seen that as the concentration of the anti-hair loss and hair-fixing composition increases, the anti-hair loss and hair-fixing effect becomes better, showing a certain concentration dependence, proving that the anti-hair loss and hair-fixing composition provided by the present invention has a good effect of hair-fixing and hair-fixing;

[0112] The anti-hair loss effect of Application Example 2 is better than that of Application Examples 4 and 5. The results show that the effect of the anti-hair loss and hair fixing composition of the present invention on improving hair loss symptoms is better than using the composite fermentation product filtrate alone or using the sericin protein concentrate alone. The anti-hair loss and hair fixing composition of the present invention has excellent anti-hair loss effect, and the composite fermentation product filtrate and the sericin protein concentrate have a certain synergistic effect.

[0113] Although the specific embodiments of the present invention are described above, it should be understood by those skilled in the art that this is only for illustration and the protection scope of the present invention is defined by the appended claims. Those skilled in the art may make various changes or modifications to these embodiments without departing from the principles and essence of the present invention, but these changes and modifications all fall within the protection scope of the present invention.

Claims

1. A composition for preventing hair loss and strengthening hair, characterized in that: By mass, it includes the following components: 15-18 parts of composite fermentation product filtrate; 1 part of silk fibroin concentrate; The method for preparing the composite fermentation product filtrate comprises the following steps: (1) Mix mulberry leaves, safflower, arborvitae leaves, agarwood, and selfheal, crush them, and sieve them to obtain a composite powder; (2) extracting the composite powder with an ethanol aqueous solution, removing the ethanol, and obtaining a crude extract; (3) using the crude extract to perform acclimatization and culture of Leuconostoc citrifolia to obtain an acclimatized composite bacterial solution; (4) inoculating the domesticated composite bacterial liquid into a fermentation medium containing the crude extract for fermentation culture, then centrifuging to obtain the supernatant, filtering and sterilizing to obtain a composite fermentation product filtrate.

2. The anti-hair loss and hair solidifying composition according to claim 1, characterized in that: In step (1), the mulberry leaves, safflower, Platycladus orientalis leaves, agarwood and selfheal are mixed according to the mass ratio of (4.5-5.5):(0.09-0.11):(6-8):(1.8-2.2):(1.8-2.2).

3. The anti-hair loss and hair solidifying composition according to claim 1, characterized in that: In step (2), the ethanol aqueous solution is a 50% v / v ethanol aqueous solution.

4. The anti-hair loss and hair solidifying composition according to claim 1, characterized in that: The step (2) is to use 50% v / v ethanol aqueous solution to extract the composite powder at room temperature for 15 days, remove the ethanol after filtering, and obtain a crude extract.

5. The anti-hair loss and hair solidifying composition according to claim 1, characterized in that: The step (3) is to inoculate the lemon-colored Leuconostoc into the acclimation culture medium containing the crude extract, and then place it in a shaking incubator at a speed of 150-200 r / min and a temperature of 25-30° C. for 1-2 days to obtain an acclimated composite bacterial solution; The step (4) is to dilute the tamed composite bacterial solution with sterile water to a viable bacterial count of 1×10 7 -1×10 9 CFU / mL of diluted bacterial solution, and then inoculated the diluted bacterial solution into the fermentation medium at an inoculation rate of 9-12% v / v, fermented and cultured for 48-60 hours at a temperature of 25-28°C and a stirring speed of 150-200 r / min, and then centrifuged to obtain the supernatant, filtered and sterilized to obtain a composite fermentation product filtrate.

6. The anti-hair loss and hair solidifying composition according to claim 5, characterized in that: The acclimation medium comprises the following components: 10 wt% crude extract, carbon source, nitrogen source, vitamins, inorganic salts, deionized water, and a pH of 6.5±0.2; The fermentation medium comprises the following components: 10 wt% of crude extract, a carbon source, a nitrogen source, vitamins, inorganic salts, deionized water, and a pH value of 6.5±0.

2.

7. Use of the anti-hair loss and hair solidifying composition according to any one of claims 1 to 6 in the preparation of a hair care product having the efficacy of preventing hair loss and hair solidifying.

8. An anti-hair loss and hair strengthening essence, characterized in that: A composition for preventing hair loss and fixing hair comprising any one of claims 1 to 6; The amount of the anti-hair loss and hair solidifying composition added to the anti-hair loss and hair solidifying essence is 10-50wt%.

9. The anti-hair loss and hair strengthening essence according to claim 8, characterized in that: The invention also comprises a fibroin concentrate, a moisturizing agent, a preservative, a thickener, an emulsifier, a pH regulator and a solvent.

10. The method for preparing the anti-hair loss and hair strengthening essence according to claim 9, characterized in that: The following steps are involved: The silk fibroin concentrate, the anti-hair loss and hair strengthening composition, moisturizer, thickener, emulsifier, preservative and solvent are mixed and stirred evenly, and then a pH regulator is added to adjust the pH to 6.5-7 to obtain an anti-hair loss and hair strengthening essence.

Images