Method for preparing high-efficiency organic liquid fertilizer by using pig and cattle breeding manure sewage

Abstract

The invention discloses a method for preparing a high-efficiency organic liquid fertilizer by using pig and cattle breeding fecal sewage, which comprises the following steps: by taking pig and cattle breeding fecal sewage as a raw material, adding a microbial agent for fermentation, then pumping the fermented to-be-treated fecal sewage into a static mixer by using a centrifugal pump, adding part of an organic particle trapping reagent for premixing, and feeding the mixture into fecal sewage treatment equipment, adding the residual organic particle trapping reagent, trapping insoluble particles which are not fermented and decomposed, and separating to remove the insoluble particles, thereby obtaining the high-efficiency organic liquid fertilizer. Compared with biogas slurry and biogas residue, the organic liquid fertilizer has high fertilizer efficiency, is convenient to store, transport and use, and can be applied to any crops in various modes such as drip irrigation, spray irrigation, spray irrigation and the like at various time periods. In a word, the process is simple, the effect is good, the method can be used as a new technology for manure sewage resource utilization, and the obtained organic liquid fertilizer is high in fertilizer efficiency and wide in application.

Description

Technical Field

[0001] The invention belongs to the technical field of organic liquid fertilizer preparation, and in particular relates to a method for preparing high-efficiency organic liquid fertilizer by utilizing pig and cattle breeding manure sewage. Background Art

[0002] Intensive animal husbandry produces a large amount of animal manure, which contains a large amount of organic matter, viruses and bacteria. Improper handling of this manure will have direct and indirect impacts on the ecological environment such as water bodies, atmosphere, and soil, including water pollution, air pollution, soil pollution, and microbial pollution. Therefore, a large amount of breeding waste has not been effectively treated and utilized, which has become a major problem in rural environmental governance. According to the 2020 "Second National Pollution Source Census Bulletin", pollution emissions from livestock and poultry breeding have become one of the most important agricultural non-point source pollution sources in my country.

[0003] At present, there are many methods for treating livestock manure, but there are very few methods suitable for effectively treating manure and sewage. The resource utilization of manure and sewage is one of the main methods vigorously promoted by the country. This method is to produce biogas through anaerobic fermentation of manure and sewage, and the generated biogas slurry and biogas residue are used for returning to the field. This mixture of biogas slurry and biogas residue has many problems in storage, transportation and application due to the sedimentation of biogas residue. It is deposited at the bottom of the container, which is not convenient for discharge or cleaning. It is deposited in the pipeline, causing pipeline blockage. When applied, it adheres to the surface of crops, causing crop damage, and accumulates on the soil surface. With the erosion of rainwater, it pollutes the water body. Therefore, the crop varieties, application time, application method and application range of biogas slurry and biogas residue are all limited. It is basically only suitable for use in the rice transplanting link that can be transported by pumping pipelines nearby. At the same time, in the process of anaerobic fermentation to produce biogas, most of the easily fermentable organic matter is converted into biogas, and the remaining biogas residue is only some lignin and cellulose that are difficult to ferment and decompose, as well as some sediments that are difficult to dissolve in water, so the fertilizer effect of biogas slurry and biogas residue has been greatly reduced. Summary of the invention

[0004] The technical problem to be solved by the present invention is to provide a method for preparing high-efficiency organic liquid fertilizer by utilizing pig and cattle breeding manure and sewage with simple process and good effect. The obtained organic liquid fertilizer has high fertilizer efficiency and wide application.

[0005] In order to solve the above technical problems, the present invention adopts the following technical solutions:

[0006] The invention discloses a method for preparing high-efficiency organic liquid fertilizer by using pig and cattle breeding manure and sewage. The pig and cattle breeding manure and sewage are used as raw materials, microbial agents are added for fermentation to produce various water-soluble small molecular organic matters, humic acid, fulvic acid, polypeptides, amino acids and biological enzymes, and then a centrifugal pump is used to pump the fermented manure and sewage to be treated to a static mixer, and part of the organic particle capturing agent is added for premixing, and then the manure and sewage are sent to a manure and sewage treatment device, and the remaining organic particle capturing agent is added to capture insoluble particles that have not been fermented and decomposed, and then the insoluble particles are separated and removed to obtain high-efficiency organic liquid fertilizer.

[0007] The fecal sewage treatment equipment is the Chinese patented "Organic Sewage Treatment System", and its patent number is 2019201762284.

[0008] Part of the organic particle trapping agent is 70-80% of the required amount, and the remaining amount is the remaining required amount. The organic particle trapping agent is polyacrylamide.

[0009] The dosage of the microbial agent is 0.2kg / cubic meter of the fecal wastewater to be treated; the fermentation is carried out at room temperature and the fermentation time is 3 to 7 days (the specific time depends on the ambient temperature and target requirements).

[0010] The microbial agent is obtained by co-culturing two strains of Clostridium cellulovorans and Syntrophomonas wolfei, and then acclimating, culturing and proliferating them with fecal sewage.

[0011] The co-cultivation was performed as follows: Cellulomonas and psychrophilic synthetic bacteria were activated separately, then mixed at a ratio of 1:1, 50 mL was inoculated into 1000 mL of anaerobic culture medium, and co-cultivation was performed under anaerobic conditions at a temperature of 25°C, a culture time of 72 h, and a stirring rate of 50 rpm;

[0012] The acclimation culture was carried out as follows: the co-cultured bacterial solution was mixed with the aquaculture wastewater at a ratio of 5:1, and placed in an anaerobic tank for acclimation culture at a temperature of 25°C, a culturing time of 72 hours, and a stirring rate of 50 rpm;

[0013] The proliferation and expansion operation is carried out as follows: the domesticated and cultured bacterial solution is mixed with aquaculture manure and sewage at a ratio of 1:5, and placed in an anaerobic tank for proliferation and expansion, with a culture temperature of 25°C, a culture time of 72h, and a stirring rate of 50rpm; the bacterial solution obtained from the proliferation and expansion is the microbial agent.

[0014] The anaerobic culture medium was prepared as follows: one liter of the culture medium contained 5 g of glucose, 10 g of cellulose powder, 2 g of sodium butyrate, 0.5 g of NH4Cl, 0.5 g of KH2PO4, 0.2 g of MgSO4·7H2O, 0.2 g of CaSO4·2H2O, 2.0 g of FeCl2·4H2O, 1.0 g of CoCl2·6H2O, 0.5 g of MnCl2·4H2O, 0.8 g of NiCl2·6H2O, 0.3 g of ZnCl2, 0.05 g of CuCl2·2H2O, 0.3 g of H3BO3, and 0.5 g of (NH4)6Mo7O 24 ·4H2O 0.8g, EDTA (disodium) 1.0g, adjust pH to 6.8-7.2 with NaHCO3.

[0015] The high-efficiency organic liquid fertilizer prepared by the above method.

[0016] The liquid fertilizer contains suspended solids of particles larger than 0.45 μm not exceeding 100 mg / L, organic matter content of 1.5-5.5 g / L, total nitrogen content of 0.5-2.5 g / L, total phosphorus pentoxide of 0.05-1.0 g / L, total potassium oxide of 0.8-7.5 g / L, and total oxygen content of 2.0-10.0 g / L.

[0017] The high-efficiency organic liquid fertilizer is used for planting crops.

[0018] In response to the current problems in the treatment of livestock manure and sewage, the inventor has established a method for preparing high-efficiency organic liquid fertilizer using pig and cattle manure and sewage. Pig and cattle manure and sewage are used as raw materials, microbial agents are added for fermentation, and then a centrifugal pump is used to pump the fermented manure and sewage to be treated to a static mixer, and some organic particle capture agents are added for pre-mixing, and then the manure and sewage are sent to the manure and sewage treatment equipment, and the remaining organic particle capture agents are added to capture insoluble particles that have not been fermented and decomposed, and then the insoluble particles are separated and removed to obtain high-efficiency organic liquid fertilizer.

[0019] Studies have shown that the organic liquid fertilizer obtained by this method contains a variety of water-soluble small molecular organic matter, humic acid, fulvic acid, polypeptides, amino acids, biological enzymes, a variety of active microorganisms, soluble nitrogen, phosphorus, potassium and other nutrients, and the product contains almost no insoluble particles. Compared with biogas slurry and biogas residue, this organic liquid fertilizer has high fertilizer efficiency and is easy to store, transport and use. It can be applied to any crop at various times using drip irrigation, drench irrigation, spraying and other methods.

[0020] In conclusion, the process of the present invention is simple and has good effects, and it can be used as a new technology for resource utilization of feces and sewage. The obtained organic liquid fertilizer has high fertilizer efficiency and wide application. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1The present invention is a process flow chart of a method for preparing high-efficiency organic liquid fertilizer by utilizing pig and cattle breeding manure sewage. DETAILED DESCRIPTION

[0022] Embodiment 1

[0023] 1. Preparation of special microbial agents

[0024] The two strains of Cellulomonas and Psychrophilic Synthetic Bacteria were first co-cultured, and then domesticated and cultured with fecal sewage, and then multiplied and expanded. The specific operation is as follows:

[0025] Co-cultivation: Cellulomonas and psychrophilic synthetic bacteria were activated separately, then mixed at a ratio of 1:1, 50 mL was inoculated into 1000 mL of anaerobic culture medium, and co-cultivated under anaerobic conditions at a temperature of 25°C, a culture time of 72 h, and a stirring rate of 50 rpm;

[0026] The anaerobic culture medium was prepared as follows: one liter of the culture medium contained 5 g of glucose, 10 g of cellulose powder, 2 g of sodium butyrate, 0.5 g of NH4Cl, 0.5 g of KH2PO4, 0.2 g of MgSO4·7H2O, 0.2 g of CaSO4·2H2O, 2.0 g of FeCl2·4H2O, 1.0 g of CoCl2·6H2O, 0.5 g of MnCl2·4H2O, 0.8 g of NiCl2·6H2O, 0.3 g of ZnCl2, 0.05 g of CuCl2·2H2O, 0.3 g of H3BO3, and 0.5 g of (NH4)6Mo7O 24 · 4H2O 0.8g, EDTA (disodium) 1.0g, use NaHCO3 to adjust pH to 6.8-7.2. During the preparation process, heat to drive out dissolved oxygen, and purge with nitrogen to completely deoxygenate and ensure an anaerobic environment.

[0027] Acclimation culture: Mix the co-cultured bacterial liquid with aquaculture wastewater (filtered through a 100-mesh screen) at a ratio of 5:1 and place in an anaerobic tank for acclimation culture at a temperature of 25°C, a culture time of 72 hours, and a stirring rate of 50 rpm;

[0028] Propagation and expansion: Mix the domesticated and cultured bacterial liquid with aquaculture wastewater (filtered through a 100-mesh sieve) at a ratio of 1:5, and put it into an anaerobic tank for proliferation and expansion. The culture temperature is 25°C, the culture time is 72h, and the stirring rate is 50rpm. The bacterial liquid obtained from the proliferation and expansion is the special microbial agent.

[0029] 2. Fermentation

[0030] The sewage discharged from the pig farm is collected into a fermentation tank, and the special microbial agent is added to the sewage to be treated at a ratio of 0.2 kg per cubic meter of sewage to be treated. The mixture is stirred evenly and fermented at room temperature for 7 days.

[0031] 3. Capture and separation

[0032] The fermented fecal sewage to be treated is pumped to a static mixer using a centrifugal pump, and the required amount of 75% organic particle capture agent (i.e., polyacrylamide, the same in the following cases) is added for premixing; the mixture premixed by the static mixer is sent to a dedicated fecal sewage treatment equipment (Chinese patent "Organic Sewage Treatment System", whose patent number is 2019201762284), the remaining organic particle capture agent is added, and the fecal sewage treatment equipment is started for treatment. After the treatment of the fecal sewage treatment equipment, the insoluble particles in the fecal sewage that have not been fermented and decomposed are separated from the aqueous solution, and the separated aqueous solution is the organic liquid fertilizer product. The separated solids are treated separately and used to produce organic solid fertilizer products.

[0033] 4. Quality Inspection

[0034] Tests show that the liquid fertilizer contains 87 mg / L suspended matter above 0.45 μm, 3.1 g / L organic matter, 1.4 g / L total nitrogen, 0.5 g / L total phosphorus pentoxide, 4.5 g / L total potassium oxide and 6.4 g / L total nutrients.

[0035] 5. Product Application

[0036] Two plots of land with the same soil quality were selected from a vegetable planting base and divided into an experimental group and a control group. Both groups planted the same variety of screw peppers. The experimental group only applied the organic liquid fertilizer obtained in this embodiment, and the control group only applied the commonly used commercial water-soluble fertilizer (N: 100g / L, P2O5: 200g / L, K2O: 200g / L, total nutrients: 500g / L). Both the experimental group and the control group followed the traditional planting method and carried out normal field management until the end of the harvest. The results are shown in Table 1.

[0037] Table 1

[0038] serial number Area, mu Variety name Number of plants Total output, kg Yield per mu, kg Fertilizer cost, yuan / mu Experimental Group 0.29 Screw pepper 37-94 400 2569 8859 1650 Control group 0.29 Screw pepper 37-94 400 2568 8855 7500

[0039] It can be seen from Table 1 that, when the output of screw pepper is roughly the same, the application of the organic liquid fertilizer provided by the present invention can reduce the fertilizer cost by 78.0% compared with the application of the commonly used commercial water-soluble fertilizer.

[0040] Embodiment 2

[0041] 1. Preparation of special microbial agents

[0042] Same as Example 1.

[0043] 2. Fermentation

[0044] The method is basically the same as Example 1, except that the raw material is sewage discharged from a cattle farm, and the fermentation time is 5 days.

[0045] 3. Capture and separation

[0046] Same as Example 1.

[0047] 4. Quality Inspection

[0048] Tests show that the liquid fertilizer contains 73 mg / L suspended matter above 0.45 μm, 1.98 g / L organic matter, 0.8 g / L total nitrogen, 0.8 g / L total phosphorus pentoxide, 7.3 g / L total potassium oxide and 8.9 g / L total nutrients.

[0049] 5. Product Application

[0050] Two plots of land with the same soil quality were selected from a vegetable planting base and divided into an experimental group and a control group. The same variety of cucumbers were planted in both groups. The experimental group only applied the organic liquid fertilizer obtained in this embodiment, and the control group only applied the commonly used commercial water-soluble fertilizer (same as in Example 1). Both the experimental group and the control group were managed normally in the field according to the traditional planting method until the harvest was completed. The results are shown in Table 2.

[0051] Table 2

[0052] serial number Area, mu Variety name Number of plants Total output, kg Yield per mu, kg Fertilizer cost, yuan / mu Experimental Group 3.49 cucumber 8377 28118 8057 1650 Control group 1.92 cucumber 4608 14174 7382 7500

[0053] As shown in Table 2, the use of the organic liquid fertilizer provided by the present invention to grow cucumbers can increase the per-acre yield of cucumbers by 9.1% while reducing the fertilizer cost by 78.0%, compared with the use of commonly used commercial water-soluble fertilizers.

[0054] Embodiment 3

[0055] 1. Preparation of special microbial agents

[0056] Same as Example 1.

[0057] 2. Fermentation

[0058] The method is basically the same as that of Example 1, except that the fermentation time is 4 days.

[0059] 3. Capture and separation

[0060] Same as Example 1.

[0061] 4. Quality Inspection

[0062] Tests show that the liquid fertilizer contains 84 mg / L suspended matter above 0.45 μm, 5.0 g / L organic matter, 2.0 g / L total nitrogen, 0.05 g / L total phosphorus pentoxide, 1.0 g / L total potassium oxide and 3.0 g / L total nutrients.

[0063] 5. Product Application

[0064] In a sugar cane planting base, in the 2022-2023 crushing season, the traditional compound fertilizer (nutrient content: 17-6-13, total nutrients: 36%) was used for planting, and the average yield per mu was 4.1 tons / mu. In the 2023-2024 crushing season, the organic liquid fertilizer provided by the present invention was used for planting, and the fertilizer cost remained the same as the previous crushing season, both at 600 yuan / mu, and the average yield per mu was 6.4 tons / mu.

[0065] Compared with using traditional compound fertilizers, the use of the organic liquid fertilizer provided by the present invention to plant sugar cane can increase the yield per mu by 56.1%, thereby significantly increasing the yield of sugar cane.

Claims

1. A method for preparing high-efficiency organic liquid fertilizer using pig and cattle breeding manure sewage, characterized in that: Using pig and cattle manure and sewage as raw materials, add microbial agents for fermentation, and then use a centrifugal pump to pump the fermented manure and sewage to be treated to a static mixer, add some organic particle capture agents for premixing, and then send it to the manure and sewage treatment equipment, add the remaining organic particle capture agents, capture the insoluble particles that have not been fermented and decomposed, and then separate and remove the insoluble particles to obtain high-efficiency organic liquid fertilizer.

2. The method according to claim 1, characterized in that: The fecal sewage treatment equipment is the Chinese patent "organic sewage treatment system", and its patent number is 2019201762284.

3. The method according to claim 1, characterized in that: The part of the organic particle capturing agent is 70-80% of the required amount, and the remaining amount of the organic particle capturing agent is the remaining required amount; the organic particle capturing agent is polyacrylamide.

4. The method according to claim 1, characterized in that: The dosage of the microbial agent is 0.2 kg / m3 of the fecal wastewater to be treated; the fermentation is performed at room temperature and the fermentation time is 3 to 7 days.

5. The method according to claim 4, characterized in that: The microbial agent is obtained by first co-culturing two strains of Cellulomonas and psychrophilic synthetic bacteria, and then acclimating, culturing, and proliferating and expanding the culture with fecal sewage.

6. The method according to claim 5, characterized in that: The co-cultivation is performed as follows: Cellulomonas and psychrophilic synthetic bacteria are activated separately, then mixed at a ratio of 1:1, 50 mL is inoculated into 1000 mL of anaerobic culture medium, and co-cultivated under anaerobic conditions at a temperature of 25° C., a culture time of 72 h, and a stirring rate of 50 rpm; The acclimation culture is performed as follows: the co-cultured bacterial solution is mixed with the aquaculture wastewater in a ratio of 5:1, and placed in an anaerobic tank for acclimation culture at a temperature of 25°C, a culturing time of 72 hours, and a stirring rate of 50 rpm; The proliferation and expansion is carried out as follows: the domesticated and cultured bacterial solution is mixed with aquaculture manure and sewage at a ratio of 1:5, and placed in an anaerobic tank for proliferation and expansion, with a culture temperature of 25° C., a culture time of 72 h, and a stirring rate of 50 rpm; the bacterial solution obtained by proliferation and expansion is the microbial agent.

7. The method according to claim 6, characterized in that The anaerobic culture medium was prepared as follows: one liter of the culture medium contained 5 g of glucose, 10 g of cellulose powder, 2 g of sodium butyrate, 0.5 g of NH4Cl, 0.5 g of KH2PO4, 0.2 g of MgSO4·7H2O, 0.2 g of CaSO4·2H2O, 2.0 g of FeCl2·4H2O, 1.0 g of CoCl2·6H2O, 0.5 g of MnCl2·4H2O, 0.8 g of NiCl2·6H2O, 0.3 g of ZnCl2, 0.05 g of CuCl2·2H2O, 0.3 g of H3BO3, and (NH4)6Mo7O 24 ·4H2O 0.8g, EDTA (disodium) 1.0g, adjust pH to 6.8-7.2 with NaHCO3.

8. the high-efficiency organic liquid fertilizer that the arbitrary described method of claim 1 to 7 makes.

9. The high-efficiency organic liquid fertilizer according to claim 8, characterized in that: The liquid fertilizer has a suspended solid content of particles larger than 0.45 μm not exceeding 100 mg / L, an organic matter content of 1.5 to 5.5 g / L, a total nitrogen content of 0.5 to 2.5 g / L, a total phosphorus pentoxide content of 0.05 to 1.0 g / L, a total potassium oxide content of 0.8 to 7.5 g / L, and a total nutrient content of 2.0 to 10.0 g / L.

10. The high-efficiency organic liquid fertilizer according to any one of claims 8 to 9 is used for crop planting.

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